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Pesquisa : G16.824 [Categoria DeCS]
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Id: biblio-1291632
Autor: Li, Yingnuo; Yanshuang, Wang; Mingcheng, Li; Lihua, Zhang; Guang Xin, Yuan.
Título: Development of a species-specific PCR assay for authentication of Agkistrodon acutus based on mitochondrial cytochrome b gene
Fonte: Electron J Biotechnol;49:29-33, Jan. 2021. tab, ilus.
Idioma: en.
Projeto: High technical industry of development and Reform Commission of Jilin Province; . Beihua University Young Teacher Promotion Program; . Beihua University Graduate Innovation Program.
Resumo: BACKGROUND: Agkistrodon acutus, a traditional Chinese medicine, clinically used in the treatment of rheumatism, tumor, and cardiovascular and cerebrovascular diseases. Due to the unique medicinal value and the difficulty of artificial breeding of Agkistrodon acutus, the supply of Agkistrodon acutus on the market exceeds the demand, and a large number of its adulterants are found on the market. In this study, the cytb gene sequences of Agkistrodon acutus and 9 snakes were compared and analyzed, specific primers were designed, and specific PCR methods were established to detect Agkistrodon acutus medicinal samples on the market. RESULTS: This method was successfully applied to distinguish the snake from other adulterated species, and tested 18 Agkistrodon acutus samples randomly purchased from six cities. Twelve samples were counterfeit and six were genuine. The standard reference material of Agkistrodon acutus was cloned by molecular cloning and sequencing, and the gene sequence difference with other species was significant. It shows that the region could be used as the fingerprint region of the target species. CONCLUSIONS: The proposed method can be used as a species-specific marker and can be highly distinguished from other adulterated snake species, which is helpful to effectively avoid the problem of false sale of Agkistrodon acutus.
Descritores: Reação em Cadeia da Polimerase/métodos
Agkistrodon/genética
Citocromos b/genética
Mitocôndrias/genética
-Serpentes
Especificidade da Espécie
DNA/análise
Clonagem Molecular
Medicina Tradicional Chinesa
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  2 / 879 LILACS  
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Id: biblio-1319419
Autor: Camacho Ocsachoque, Nelly.
Título: Tipologías alimenticias en las especies de animales: ovejas, vacunos y equinos / sss.
Fonte: Cochabamba; s.n; 2005. 65 p. tab.
Idioma: es.
Tese: Apresentada a Universidad Mayor de San Simon. Facultad de Ciencias y Tecnología para obtenção do grau de Especialidad en Estadística.
Resumo: La actividad agricola regional confronta diversas limitaciones de tipo ecologico del sistema de producción y de carácter socio-económico. Sin embargo existen algunas alternativas agrícolas que económicamente pueden ser expentantes para el pequeño productor de la zona altiplanica de Bolivia. Dentro de las alternativas, se tienen la producción de forrajes y de biomasa en las praderas nativas especialmente para la alimentación de equinos, vacunos y ovinos. En este sentido los problemas que deben considerarse son: baja calidad y cantidad de forrajes producidos y bajo rendimientos de biomasa. Dentro del análisis multivariado se han considerado los métodos de análisis discriminante Conónico. Análisis de varianza y el análisis Regresional, para caracterizar y optmizar la producción de forrajes y la selección de pastoreo para cada especie..
Descritores: Especificidade da Espécie
Estatística
Responsável: BO134.1 - Biblioteca
01025 TEC.P3016


  3 / 879 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: biblio-838972
Autor: Espinoza, Rodrigo A; Silva-Valenzuela, Cecilia A; Amaya, Fernando A; Urrutia, Ítalo M; Contreras, Inés; Santiviago, Carlos A.
Título: Differential roles for pathogenicity islands SPI-13 and SPI-8 in the interaction of Salmonella Enteritidis and Salmonella Typhi with murine and human macrophages
Fonte: Biol. Res;50:5, 2017. tab, graf.
Idioma: en.
Projeto: FONDECYT; . FONDECYT; . CONICYT; . CONICYT; . CONICYT.
Resumo: BACKGROUND: Salmonella pathogenicity island (SPI)-13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Typhimurium and S. Gallinarum. However, it is absent in typhoid serovars such as S. Typhi and Paratyphi A, which carry SPI-8 at the same genomic location. Because the interaction with macrophages is a critical step in Salmonella pathogenicity, in this study we investigated the role played by SPI-13 and SPI-8 in the interaction of S. Enteritidis and S. Typhi with cultured murine (RAW264.7) and human (THP-1) macrophages. RESULTS: Our results showed that SPI-13 was required for internalization of S. Enteritidis in murine but not human macrophages. On the other hand, SPI-8 was not required for the interaction of S. Typhi with human or murine macrophages. Of note, the presence of an intact copy of SPI-13 in a S. Typhi mutant carrying a deletion of SPI-8 did not improve its ability to be internalized by, or survive in human or murine macrophages. CONCLUSIONS: Altogether, our results point out to different roles for SPI-13 and SPI-8 during Salmonella infection. While SPI-13 contributes to the interaction of S. Enteritidis with murine macrophages, SPI-8 is not required in the interaction of S. Typhi with murine or human macrophages. We hypothesized that typhoid serovars have lost SPI-13 and maintained SPI-8 to improve their fitness during another phase of human infection.
Descritores: Salmonella enteritidis/genética
Infecções por Salmonella/microbiologia
Salmonella typhi/genética
Ilhas Genômicas/fisiologia
Macrófagos/microbiologia
-Especificidade da Espécie
Sobrevivência Celular
Células Cultivadas
Reação em Cadeia da Polimerase
Análise de Variância
Genoma Bacteriano
Fenômenos Fisiológicos Bacterianos
Ilhas Genômicas/genética
Interações Microbianas/genética
Sorogrupo
Células RAW 264.7
Muridae
Limites: Humanos
Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central


  4 / 879 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: biblio-1019708
Autor: Monteiro, Daniela Cristina Sensato; Souza, Natália Vasconcelos de; Amaral, Jane Cavalcante; Lima, Kaynan Bezerra de; Araújo, Fernanda Montenegro Carvalho de; Ramalho, Izabel Letícia Cavalcante; Martins, Victor Emanuel Pessoa; Colares, Jeová Keny Baima; Cavalcanti, Luciano Pamplona de Góes; Lima, Danielle Malta.
Título: Dengue: 30 years of cases in an endemic area
Fonte: Clinics;74:e675, 2019. tab, graf.
Idioma: en.
Resumo: The present study aimed to review literature on studies of dengue cases conducted over 30 years in the state of Ceará. Between November 2015 and January 2016, articles published in Portuguese and English in 7 databases were searched using keywords and a Boolean operator. A total of 191 articles were identified in the databases; 133 were excluded according to the exclusion criteria, and 58 were included in the study. Of the 58 articles analyzed, 6 reported data from Brazil; including the Northeast region and the state of Ceará; 41 reported data for only the city of Fortaleza; 7 reported data for the state of Ceará; 4 reported data for cities in the interior of the state; and 3 included only children. The studies adopted different approaches and focused on different aspects of the disease. Study outcomes included the identification of serological, epidemiological, clinical, and laboratory characteristics; potential larvicides and biological predators of mosquitoes; potential antiviral agents; vector density characteristics; and educational dengue prevention and control strategies. Additionally, one vaccine trial was included. Although studies on dengue in the state of Ceará are scarce, they are encompassing, including several lines of research, and the number of studies and reports on dengue in the state of Ceará continues to increase.
Descritores: Doenças Endêmicas/estatística & dados numéricos
Aedes/classificação
Dengue/epidemiologia
Mosquitos Vetores/fisiologia
-Comportamento Predatório
Projetos de Pesquisa
Especificidade da Espécie
Brasil/epidemiologia
Doenças Endêmicas/prevenção & controle
Aedes/fisiologia
Dengue/prevenção & controle
Dengue/transmissão
Limites: Humanos
Animais
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Araújo, Silvana Marques de
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Id: biblio-897012
Autor: Dworak, Elaine Schultz; Araújo, Silvana Marques de; Gomes, Mônica Lúcia; Massago, Miyoko; Ferreira, Érika Cristina; Toledo, Max Jean de Ornelas.
Título: Sympatry influence in the interaction of Trypanosoma cruzi with triatomine
Fonte: Rev. Soc. Bras. Med. Trop;50(5):629-637, Sept.-Oct. 2017. tab, graf.
Idioma: en.
Projeto: Araucaria Foundation for Scientific and Technological Development; . National Council for Scientific and Technological Development.
Resumo: Abstract INTRODUCTION: Trypanosoma cruzi, the etiologic agent of Chagas disease, is widely distributed in nature, circulating between triatomine bugs and sylvatic mammals, and has large genetic diversity. Both the vector species and the genetic lineages of T. cruzi present a varied geographical distribution. This study aimed to verify the influence of sympatry in the interaction of T. cruzi with triatomines. Methods: The behavior of the strains PR2256 (T. cruzi II) and AM14 (T. cruzi IV) was studied in Triatoma sordida (TS) and Rhodnius robustus (RR). Eleven fifth-stage nymphs were fed by artificial xenodiagnosis with 5.6 × 103 blood trypomastigotes/0.1mL of each T. cruzi strain. Every 20 days, their excreta were examined for up to 100 days, and every 30 days, the intestinal content was examined for up to 120 days, by parasitological (fresh examination and differential count with Giemsa-stained smears) and molecular (PCR) methods. Rates of infectivity, metacyclogenesis and mortality, and mean number of parasites per insect and of excreted parasites were determined. RESULTS: Sympatric groups RR+AM14 and TS+PR2256 showed higher values of the four parameters, except for mortality rate, which was higher (27.3%) in the TS+AM14 group. General infectivity was 72.7%, which was mainly proven by PCR, showing the following decreasing order: RR+AM14 (100%), TS+PR2256 (81.8%), RR+PR2256 (72.7%) and TS+AM14 (36.4%). CONCLUSIONS: Our working hypothesis was confirmed once higher infectivity and vector capacity (flagellate production and elimination of infective metacyclic forms) were recorded in the groups that contained sympatric T. cruzi lineages and triatomine species.
Descritores: Vetores Artrópodes/fisiologia
Rhodnius/fisiologia
Triatoma/fisiologia
Trypanosoma cruzi/fisiologia
Simpatria
-Vetores Artrópodes/genética
Vetores Artrópodes/patogenicidade
Rhodnius/genética
Rhodnius/patogenicidade
Especificidade da Espécie
Fatores de Tempo
Triatoma/genética
Triatoma/patogenicidade
Trypanosoma cruzi/genética
Trypanosoma cruzi/patogenicidade
Sangue/parasitologia
Brasil
Reação em Cadeia da Polimerase
Doença de Chagas/parasitologia
Doença de Chagas/transmissão
Xenodiagnóstico/métodos
Interações Hospedeiro-Parasita/fisiologia
Intestinos/parasitologia
Camundongos
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Texto completo
Id: biblio-1041406
Autor: Benacer, Douadi; Zain, Siti Nursheena Mohd; Lewis, John W; Khalid, Mohd Khairul Nizam Mohd; Thong, Kwai Lin.
Título: A duplex endpoint PCR assay for rapid detection and differentiation of leptospira strains
Fonte: Rev. Soc. Bras. Med. Trop;50(2):239-242, Mar.-Apr. 2017. tab, graf.
Idioma: en.
Projeto: Malaya High Impact Research Grant; . University of Malaya.
Resumo: Abstract INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. RESULTS: The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water. CONCLUSIONS: Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.
Descritores: DNA Bacteriano/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Primers do DNA
Leptospira/classificação
-Especificidade da Espécie
Sensibilidade e Especificidade
Leptospira/isolamento & purificação
Leptospira/genética
Responsável: BR1.1 - BIREME


  7 / 879 LILACS  
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Lonardoni, Maria Valdrinez Campana
Texto completo
Id: biblio-1041471
Autor: Santos, Amanda Gubert Alves dos; Ferlini, Jéssica de Paula; Vicentino, Suellen Lais; Lonardoni, Maria Valdrinez Campana; Sant'Ana, Debora de Mello Gonçales; Melo, Gessilda de Alcântara Nogueira de.
Título: Alterations induced in the ileum of mice upon inoculation with different species of Leishmania: a preliminary study
Fonte: Rev. Soc. Bras. Med. Trop;51(4):537-541, July-Aug. 2018. tab, graf.
Idioma: en.
Projeto: Universidade Estadual de Maringá.
Resumo: Abstract INTRODUCTION: Leishmania species cause skin, mucosal, and disseminated lesions. We studied the effects of three Leishmania species on ileal morphology in mice. METHODS: BALB/c mice were intraperitoneally inoculated with Leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis, and Leishmania (Leishmania) major (4 animals/group). After 72h, the ilea were collected and histologically processed. RESULTS: Following inoculation, the goblet cell and intraepithelial lymphocyte populations increased, while Paneth cell number and crypt width decreased. In addition, enterocyte size, villi height, and mucosa, submucosa, and muscular tunic thickness increased. CONCLUSIONS: Leishmania modified the quantity of cells in and morphology of mice ilea.
Descritores: Leishmaniose/parasitologia
Leishmaniose/patologia
Íleo/parasitologia
Íleo/patologia
Leishmania/patogenicidade
Camundongos
-Especificidade da Espécie
Modelos Animais de Doenças
Leishmania/classificação
Camundongos Endogâmicos BALB C
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME


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Rosa, Joao Aristeu da
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Id: biblio-1101448
Autor: Falcone, Rossana; Ribeiro, Aline Rimoldi; Oliveira, Jader de; Mendonça, Vagner José; Graminha, Márcia; Rosa, João Aristeu da.
Título: Differentiation of Rhodnius neglectus and Rhodnius prolixus (Hemiptera: Reduviidae: Triatominae) by multiple parameters
Fonte: Rev. Soc. Bras. Med. Trop;53:e20190503, 2020. tab, graf.
Idioma: en.
Projeto: Coordination for the Improvement of Higher Education Personnel.
Resumo: Abstract Introduction: The genus Rhodnius in the subfamily Triatominae comprises 20 species, which can transmit Trypanosoma cruzi and Trypanosoma rangeli. Due to the development of molecular techniques, Triatominae species can now be characterized by mitochondrial and nuclear markers, making it possible to verify and/or correct the existing data on these species. The results achieved in this study provide a more detailed and accurate differentiation of the Rhodnius species, helping the establishment of a more appropriate classification. Methods: Data collection was performed by DNA analysis, morphological and morphometric studies to distinguish four populations of R. neglectus and four of R. prolixus. Phylogenetic data were compared to morphological and morphometric data. Results: The analysis of Cytb fragments suggests that the four colonies designated to Rhodnius neglectus as well as those of R. prolixus were correctly identified. Conclusions: The morphological characters observed in the specimens of the colonies originally identified as R. prolixus and R. neglectus, such as the presence or absence of collar in the eggs, the patterns of the median process of the pygophore, and anterolateral angle, are consistent with the species. Geometric morphometrics also show an intraspecific variability in R. prolixus.
Descritores: Rhodnius/classificação
Insetos Vetores/anatomia & histologia
-Filogenia
Rhodnius/anatomia & histologia
Rhodnius/genética
Especificidade da Espécie
Doença de Chagas/transmissão
Análise de Sequência de DNA
Insetos Vetores/classificação
Insetos Vetores/genética
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


  9 / 879 LILACS  
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Id: biblio-1171793
Autor: Barrera Viviana A; Kageyama Koji; Rojo Rodrigo A; Gasoni Laura; Kobayashi Kiroku.
Título: A species-specific method for detecting pathogenic Streptomyces species from soil and potato tubers in Argentina / A species-specific method for detecting pathogenic Streptomyces species from soil and potato tubers in Argentina.
Fonte: Rev. argent. microbiol;45(4):277-81, dic. 2013.
Idioma: es.
Resumo: Potato common scab is caused by several soil-inhabiting pathogenic Streptomyces species. In the present study, a species-specific PCR method was used to detect Streptomyces species in potato tuber lesions and soils. Total genomic DNA from soil samples from six locations and tuber samples from four potato cultivars (Spunta, Shepody, Innovator and Russet Burbank) were assessed. Streptomyces scabies, Streptomyces acidiscabies, and Streptomyces turgidiscabies were detected in soybean, tobacco and potato soils and in all potato varieties except Russet Burbank. The phylogenetic analysis of the sequences obtained confirmed the identification. The method proposed proved to be time-saving and cost effective for the rapid detection of Streptomyces species. This is the first report of the detection of S. acidiscabies and S. turgidiscabies in soils and potato tubers from Argentina.
Descritores: Doenças das Plantas/microbiologia
Microbiologia do Solo
Solanum tuberosum/microbiologia
Streptomyces/isolamento & purificação
-Argentina
Especificidade da Espécie
Técnicas Bacteriológicas/métodos
Tipo de Publ: Artigo de Revista
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


  10 / 879 LILACS  
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Id: biblio-1171769
Autor: Busquets Natalia P; Baroni María R; Ochoteco María C; Zurbriggen María L; Virgolini Stella; Meneghetti Fernando G.
Título: Aislamientos bacterianos de muestras respiratorias de pacientes pediátricos con fibrosis quística y su distribución por edades / [Bacterial isolates from respiratory samples of pediatric patients with cystic fibrosis and their distribution by ages].
Fonte: Rev. argent. microbiol;45(1):44-9, mar. 2013.
Idioma: es.
Resumo: The bacterial isolates from respiratory samples of 50 pediatric patients with cystic fibrosis, their distribution by ages and antimicrobial resistance pattern as well as the intermittence of isolations and coinfections, were investigated. Staphylococcus aureus was isolated in 72

of patients, followed by Pseudomonas aeruginosa (58

), and the Burkholderia cepacia complex (12

). The frequency of resistance of P. aeruginosa isolates to ß-lactam antibiotics was low (13.8

). Fifty percent of S. aureus isolates was methicillin-resistant, and 57.1

of H. influenza was ampicillin resistant due to ß-lactamase production. In children under 4 years-old, S. aureus was predominant, followed by P. aeruginosa and H. influenzae. This order of predominance was observed in all the groups studied, except in that of children between 10 and 14 years-old. Stenotrophomonas maltophilia and Achromobacter xylosoxidans isolates were intermittent and accompanied by other microorganisms. Finally, we observed a great variety of bacterial species, which imposes stringent performance requirements for microbiological studies in all respiratory samples of these patients.
Descritores: Bactérias Gram-Negativas/isolamento & purificação
Fibrose Cística/complicações
Infecções Respiratórias/microbiologia
Sistema Respiratório/microbiologia
Staphylococcus aureus/isolamento & purificação
-Adolescente
Bactérias Gram-Negativas/efeitos dos fármacos
Coinfecção/epidemiologia
Coinfecção/microbiologia
Criança
Escarro/microbiologia
Especificidade da Espécie
Estudos Retrospectivos
Faringe/microbiologia
Farmacorresistência Bacteriana Múltipla
Fatores Etários
Feminino
Fibrose Cística/microbiologia
Humanos
Infecções Estafilocócicas/epidemiologia
Infecções Estafilocócicas/etiologia
Infecções Estafilocócicas/microbiologia
Infecções Respiratórias/epidemiologia
Infecções Respiratórias/etiologia
Infecções por Bactérias Gram-Negativas/epidemiologia
Infecções por Bactérias Gram-Negativas/etiologia
Infecções por Bactérias Gram-Negativas/microbiologia
Lactente
Masculino
Nasofaringe/microbiologia
Pré-Escolar
Tipo de Publ: Resumo em Inglês
Artigo de Revista
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes



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