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Id: biblio-876987
Autor: Araújo, Paula Gonçalves de; Abreu, Camila Siriero Melo; Donato, Ludmila; Almeida, Juliano; Crippa, Mauro; Dumont, Alexandra; Corsi, Érica; Santos, Ronaldo Carneiro dos.
Título: Efeito de uma associação de cepas probióticas contendo lactobacillus e bifidobacterium na modulação da microbiota intestinal em pacientes constipados / Effects of association of probiotic strains containing lactobacillus and bifidobacterium on modulation of the intestinal microbiota in constipated patients
Fonte: GED gastroenterol. endosc. dig;36(3):89-98, Jul.-Set. 2017. ilus, tab.
Idioma: pt.
Resumo: Introdução: a constipação é uma doença crônica que afeta a população mundial, podendo ser consequência do desequilíbrio da microbiota intestinal, conhecida como disbiose. Nesse contexto, os probióticos vêm sendo utilizados com o objetivo de promover o seu equilíbrio, a fim de mantê-la saudável (eubiose). Objetivo: avaliar, em um estudo duplo-cego, randomizado, placebo-controlado, se o uso de uma associação de cepas probióticas contendo lactobacillus e bifidobacterium por 28 dias pode modular a microbiota intestinal em pacientes constipados. Método: a atividade da associação de cepas probióticas foi comparada com placebo (maltodextrina) após a suplementação por 28 dias. Os pacientes foram avaliados por meio da análise de metagenômica e da melhora dos sintomas relacionados à constipação. Os dados do perfil da população de microrganismos presentes no intestino dos pacientes foram correlacionados com os resultados da avaliação dos sintomas abdominais e da percepção de bem-estar geral. O aumento do número de evacuações e a melhora do trânsito intestinal foram avaliados durante o estudo. Resultados: as proporções do gênero Bifidobacterium no grupo teste e controle foram 0.45% vs 0.24% no final do estudo, respectivamente (p<0,05). Adicionalmente, a diferença entre os grupos que receberam associação de cepas probióticas contendo lactobacillus e bifidobacterium e placebo permaneceu também significativamente alta com relação às espécies de Lactobacillus (1,21% vs 0,12%) após 28 dias de tratamento (p<0,05). Além disso, os participantes que receberam o probiótico apresentaram uma tendência de melhora sintomática baseada na comparação da sua microbiota e as respostas oriundas da avaliação dos sintomas abdominais e bem-estar geral. Nenhum evento adverso grave foi relatado. Conclusão: a formulação probiótica modulou a microbiota intestinal de forma diferente do placebo nos participantes do estudo. O consumo dos probióticos aumentou significativamente as bactérias benéficas e reduziu as potencialmente maléficas, contribuindo para o equilíbrio da microbiota intestinal.

Introduction: constipation is a chronic disease that affects the world population, being a consequence of the imbalance of the intestinal microbiota, known as dysbiosis. In light of this context, the probiotics have been used to trigger microbiota intestinal balance, in order to keep it healthy (eubiose) Aim: to evaluate in a double-blind, randomized, placebo-controlled study whether the use of association of probiotic strains containing lactobacillus and bifidobacterium for 28 days can modulate the intestinal microbiota of the constipated participants. Methods: the activity of association of probiotic strains was compared to placebo (maltodextrin) after 28 days of consumption. The patients were evaluated by using metagenomics analyses and improved constipation symptoms. The dates of microorganisms population profile into patients gut were correlated with results of abdominal symptoms evaluation and well-being perception. The increase in the number of evacuations and the intestinal transit were evaluated during the study. Results: the proportions of the genus Bifidobacterium in the test groups and the control group were 0.45% vs 0.24% at the end of the study, respectively (p<0.05). Additionally, the difference between the groups that received association of probiotic strains containing lactobacillus and bifidobacterium and the placebo remained higher for species of the genus Lactobacillus (1.21% vs 0.12%) after 28 days of use (p<0.05). Furthermore, the participants who received the probiotic had a tendency to improve symptomatic based on comparisons of their microbiota and the responses provided patients' abdominal symptoms and well-being. There were no reports of serious adverse events during the study. Conclusion: the probiotic formulation modulated the intestinal microbiota differently from the placebo in constipated participants included in this study. The use of this probiotic significantly increased beneficial bacteria and decreased potentially harmful microbes, which contributed to the maintenance of a healthy intestinal microbiota.
Descritores: Bifidobacterium
Constipação Intestinal
Probióticos
Probióticos/farmacologia
Microbioma Gastrointestinal
Microbioma Gastrointestinal/efeitos dos fármacos
Lactobacillus
-Método Duplo-Cego
Metagenômica
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Tipo de Publ: Ensaio Clínico Controlado Aleatório
Responsável: BR9.1 - Biblioteca de Ciências da Saúde Profa. Susana Schimidt


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Id: biblio-1087172
Autor: Salam, Menaka; Varma, Ajit.
Título: Bacterial community structure in soils contaminated with electronic waste pollutants from Delhi NCR, India
Fonte: Electron. j. biotechnol;41:72-80, sept. 2019. ilus, tab, graf.
Idioma: en.
Projeto: DST-SERB, Government of India, Young Scientist Scheme.
Resumo: Background: Microbial community analysis of electronic waste (e-waste)-polluted environments is of interest to understand the effect of toxic e-waste pollutants on the soil microbial community and to evaluate novel microorganisms resisting the toxic environment. The present study aims to investigate the bacterial community structure in soils contaminated with e-waste from various sites of Loni and Mandoli (National Capital Region (NCR), India) where e-waste dumping and recycling activities are being carried out for many years. Results: Interferences to soil metagenomic DNA extraction and PCR amplification were observed because of the presence of inhibiting components derived from circuit boards. Whole-metagenome sequencing on the Illumina MiSeq platform showed that the most abundant phyla were Proteobacteria and Firmicutes. Deltaproteobacteria and Betaproteobacteria were the most common classes under Proteobacteria. Denaturing gradient gel electrophoresis (DGGE) analysis of the bacterial 16S rRNA gene showed that e-waste contamination altered the soil bacterial composition and diversity. There was a decrease in the number of predominant bacterial groups like Proteobacteria and Firmicutes but emergence of Actinobacteria in the contaminated soil samples. Conclusions: This is the first report describing the bacterial community structure of composite soil samples of ewaste-contaminated sites of Loni and Mandoli, Delhi NCR, India. The findings indicate that novel bacteria with potential bioremediating properties may be present in the e-waste-contaminated sites and hence need to be evaluated further.
Descritores: Microbiologia do Solo
Bactérias/isolamento & purificação
Bactérias/genética
Resíduo Eletrônico/análise
-Poluentes do Solo
Reação em Cadeia da Polimerase
Metais Pesados
Proteobactérias/isolamento & purificação
Metagenômica
Eletroforese em Gel de Gradiente Desnaturante
Microbiota
Firmicutes/isolamento & purificação
Índia
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1116917
Autor: Uberos, José.
Título: Microbiota perinatal: Revisión de su importancia en la salud del recién nacido / Perinatal microbiota: review of its importance in newborn health
Fonte: Arch. argent. pediatr;118(3):e265-e270, jun. 2020. ilus.
Idioma: en; es.
Resumo: La metagenómica en el estudio de los ecosistemas bacterianos intestinales ha permitido definir un perfil genético funcional estándar en el recién nacido, de forma que un ecosistema bacteriano será tanto más "normal" cuanto más se parezca su perfil genético funcional a un estándar. El desarrollo de determinado enterotipo funcional en los primeros días de vida tras el parto es fundamental para que tenga lugar el cebado del sistema inmunológico con determinados antígenos bacterianos. Independientemente de si las primeras bacterias intestinales se adquieren antes o justo después del parto, la microbiota del recién nacido va a ser el resultado de una situación de simbiosis con la flora microbiana de su entorno, en especial, con la flora bacteriana de su madre. El tipo de parto, la administración de antibióticos perinatales, el entorno y la exposición nutricional, en especial, la lactancia materna, han demostrado relacionarse de forma importante con el microbioma intestinal predominante

The use of metagenomics in the study of gut bacterial ecosystems has helped to define a standard, functional genetic profile in newborn infants, so that a bacterial ecosystem will be deemed more "normal" the more similar its functional genetic profile is to a standard. The development of a specific functional enterotype in the first days of life after birth is critical for the priming of the immune system with certain bacterial antigens.Regardless of whether the first gut bacteria are acquired before or just after birth, the newborn microbiota will result from the symbiosis with the environmental microbial flora, especially with the bacterial flora of the mother. The type of delivery, the administration of perinatal antibiotics, the environment, and nutritional exposure, especially breastfeeding, have demonstrated an important relationship with the prevalent gut microbiome
Descritores: Microbiota
-Bactérias
Aleitamento Materno
Parto
Metagenômica
Microbioma Gastrointestinal
Saúde do Lactente
Limites: Humanos
Masculino
Feminino
Recém-Nascido
Tipo de Publ: Revisão
Responsável: AR94.1 - Centro de Información Pediatrica


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Id: lil-728528
Autor: Gutiérrez-Escobar, Andrés Julián; Bayona-Rojas, Martín; Barragan-Vidal, Carlos; Rojas-Lara, Sebastián; Oliveros, Ricardo.
Título: Metagenomic analysis of the gastric microbiota cultivable from a patient with gastritis concomitant with Barrett´s esophagus / Análisis metagenómico de la microbiota gástrica cultivable a partir de un paciente con gastritis concomitante con esófago de Barrett
Fonte: Rev. gastroenterol. Perú;34(3):229-235, jul. 2014. ilus, tab.
Idioma: en.
Resumo: Barrett’s esophagus is a distal metaplasia characterized by the transformation of squamous mucosa into columnar mucosa. This esophageal phenotype is a product not only of the chronic reflux of gastric acids, but also by microorganisms that colonize the oral cavity and stomach. Two classes of microbiota can be identified in Barrett’s esophagus; microbiota type I is associated with the normal esophagus and type II with an inflamed esophagus. The present study describes the gastric microbiota of a patient with antral gastritis concomitant with Barrett’s esophagus absent infection with Helicobacter pylori. Gastric biopsies were obtained following the protocol of Sydney and following ethical practices. The isolates were cultivated under microaerophilic conditions on Columbia Agar supplemented with IsoVitaleX™ and 7% sterile blood. Extracted DNA was sequenced using 454-GS and the results analyzed on the MG-RAST server. Gram negative isolates were found and bacteria resistant to levofloxacin, amoxicillin, tetracycline, erythromycin, and clarithromycin. The phyla Bacteroidetes, Firmicutes, Fusobacteria and Proteobacteria, the genus Bacteroides and the species group Bacteroides fragilis were most abundant. Functionally, the metabolism of carbohydrates, amino acids, and to a lesser extent, the metabolism of cofactors and vitamins were most dominant, and of which the enzymes β-glucosidase (EC 3.2.1.21), β-galactosidase (EC 3.2.1.23) and β-N-acetylhexosaminidase (EC 3.2.1.52) were most dominant. The findings of this study, because they are of only one case may probably suggest a possible pathogenic role, previously undescribed for Bacteroides fragilis, associated with human gastritis when concomitant esophageal pathology exists.

El esófago de Barrett es una metaplasia distal caracterizada por la transformación de la mucosa escamosa a mucosa columnar. Este fenotipo esofágico es producto no solo de la exposición crónica al reflujo de ácidos gástricos sino también a microbios colonizantes de la cavidad oral y del estómago. El esófago Barrett presenta 2 clases de microbiotas; la microbiota tipo I asociada con esófago normal y la tipo II a fenotipos esofágicos inflamatorios. En el presente estudio se describió la microbiota gástrica de una paciente con gastritis antral concomitante con esófago de Barrett sin infección por Helicobacter pylori y se obtuvieron biopsias gástricas siguiendo el protocolo de Sydney y estándares bioéticos. Los cultivos se hicieron en condiciones microaerofílicas en agar Columbia suplementados con isovitalex y sangre estéril al 7%. El ADN extraído fue sometido a secuenciación empleando 454 GS y las lecturas fueron analizadas en el servidor MG-RAST. Se obtuvieron aislamientos gram-negativos y resistentes a levofloxacina, amoxicilina, tetraciclina, eritromicina y claritromicina. Los Phylum Bacteroidetes, Firmicutes, Fusobacteria y Proteobacteria, el género Bacteroides y las especies de grupo Bacteroides fragilis fueron los más abundantes. Funcionalmente, el metabolismo de carbohidratos, aminoácidos y en menor grado el metabolismo de cofactores y vitaminas fueron los más dominantes; de los cuales las enzimas la β-glicosidasa (EC 3.2.1.21), β-galactosidasa (EC 3.2.1.23) y la β-N-acetilhexosaminidasa (EC 3.2.1.52) fueron las más dominantes. Estos resultados, por ser de un solo caso, solo podrían sugerir un posible papel patogénico no descrito para Bacterioides fragilis asociado con gastritis humana cuando existe patología esofágica concomitante.
Descritores: Esôfago de Barrett/microbiologia
Gastrite/microbiologia
Microbioma Gastrointestinal/genética
Metagenômica
Estômago/microbiologia
-Esôfago de Barrett/complicações
Gastrite/complicações
Limites: Feminino
Humanos
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: PE1.1 - Oficina Universitária de Biblioteca


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Id: biblio-1100762
Autor: Vaccaro, Carlos Alberto; Pavicic, Walter; Gadano, Adrián; Risk, Marcelo; Figari, Marcelo; Llesuy, Susana; Piñero, Tamara; Trinks, Julieta; Burgos, Valeria; Mazzini, Flavia; Arbelbide, Jorge; Basquiera, Ana; Bolontrade, Marcela; Giménez, María Isabel; Jauk, Federico; Argüero, Julieta.
Título: Investigación traslacional en microbioma (InTraMic): un área de interés común y creciente en el IMTIB, Hospital Italiano e Instituto Universitario / Translational research in microbiome (InTraMic): an area of common and growing interest at IMTIB, Hospital Italiano and Instituto Universitario
Fonte: Rev. Hosp. Ital. B. Aires (2004);40(1):17-24, mar. 2020. ilus.
Idioma: es.
Resumo: Se estima que aproximadamente 100 trillones de microorganismos (incluidos bacterias, virus y hongos) residen en el intestino humano adulto y que el total del material genético del microbioma es 100 veces superior al del genoma humano. Esta comunidad, conocida como microbioma se adquiere al momento del nacimiento a través de la flora comensal de la piel, vagina y heces de la madre y se mantiene relativamente estable a partir de los dos años desempeñando un papel crítico tanto en el estado de salud como en la enfermedad. El desarrollo de nuevas tecnologías, como los secuenciadores de próxima generación (NGS), permiten actualmente realizar un estudio mucho más preciso de ella que en décadas pasadas cuando se limitaba a su cultivo. Si bien esto ha llevado a un crecimiento exponencial en las publicaciones, los datos sobre las poblaciones Latinoamérica son casi inexistentes. La investigación traslacional en microbioma (InTraMic) es una de las líneas que se desarrollan en el Instituto de Medicina Traslacional e Ingeniería Biomédica (IMTIB). Esta se inició en 2018 con la línea de cáncer colorrectal (CCR) en una colaboración con el Colorectal Cancer Research Group del Leeds Institute of Medical Research en el proyecto Large bowel microbiome disease network: Creation of a proof of principle exemplar in colorectal cancer across three continents. A fines de 2019 se cumplió el objetivo de comprobar la factibilidad de la recolección, envío y análisis de muestras de MBF en 5 continentes, incluyendo muestras provenientes de la Argentina, Chile, India y Vietnam. Luego de haber participado de capacitaciones en Inglaterra, se ha cumplido con el objetivo de la etapa piloto, logrando efectivizar la recolección, envío y análisis metagenómico a partir de la secuenciación de la región V4 del ARNr 16S. En 2019, la línea de enfermedad de hígado graso no alcohólico se sumó a la InTraMic iniciando una caracterización piloto en el marco de una colaboración con el laboratorio Novartis. Los resultados de ese estudio, así como el de cáncer colorrectal, están siendo enviados a publicación. En 2020, con la incorporación de la línea de trasplante alogénico de células progenitoras hematopoyéticas, fue presentado un proyecto para un subsidio del CONICET que ha superado la primera etapa de evaluación. En el presente artículo se brinda una actualización sobre la caracterización taxonómica de microbioma y se describen las líneas de investigación en curso. (AU)

It is estimated that approximately 100 trillion microorganisms (including bacteria, viruses, and fungi) reside in the adult human intestine, and that the total genetic material of the microbiome is 100 times greater than that of the human genome. This community, known as the microbiome, is acquired at birth through the commensal flora of the mother's skin, vagina, and feces and remains relatively stable after two years, playing a critical role in both the state of health and in disease. The development of new technologies, such as next-generation sequencers (NGS), currently allow for a much more precise study of it than in past decades when it was limited to cultivation. Although this has led to exponential growth in publications, data on Latin American populations is almost non-existent. Translational research in microbiome (InTraMic) is one of the lines developed at the Instituto de Medicina Traslacional e Ingeniería Biomédica (IMTIB). This started in 2018 with the Colorectal Cancer Line (CRC) in a collaboration with the Colorectal Cancer Research Group of the Leeds Institute of Medical Research in the project "Large bowel microbiome disease network: Creation of a proof of principle exemplar in colorectal cancer across three continents". At the end of 2019, the objective of verifying the feasibility of collecting, sending and analyzing MBF samples on 5 continents, including samples from Argentina, Chile, India and Vietnam, was met. After having participated in training in England, the objective of the pilot stage has been met, achieving the collection, delivery and metagenomic analysis from the sequencing of the V4 region of the 16S rRNA. In 2019, the non-alcoholic fatty liver disease line joined InTraMic, initiating a pilot characterization in the framework of a collaboration with the Novartis laboratory. The results of that study, as well as that of colorectal cancer, are being published. In 2020, with the incorporation of the allogeneic hematopoietic stem cell transplantation line, a project was presented for a grant from the CONICET that has passed the first stage of evaluation. This article provides an update on the taxonomic characterization of the microbiome and describes the lines of ongoing research. (AU)
Descritores: Pesquisa Médica Translacional/organização & administração
Microbioma Gastrointestinal/genética
-Transplante Homólogo
Vietnã
Aztreonam/uso terapêutico
RNA Ribossômico 16S/análise
Neoplasias Colorretais/genética
Neoplasias Colorretais/microbiologia
Neoplasias Colorretais/epidemiologia
Classificação/métodos
Transplante de Células-Tronco Hematopoéticas
Metagenômica
Pesquisa Médica Translacional/métodos
Sequenciamento de Nucleotídeos em Larga Escala/tendências
Hepatopatia Gordurosa não Alcoólica/genética
Hepatopatia Gordurosa não Alcoólica/microbiologia
Hepatopatia Gordurosa não Alcoólica/patologia
Hepatopatia Gordurosa não Alcoólica/epidemiologia
Microbioma Gastrointestinal/fisiologia
Índia
América Latina
Sangue Oculto
Limites: Humanos
Tipo de Publ: Revisão
Responsável: AR2.1 - Biblioteca Central


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Id: biblio-974310
Autor: Souza, Renata Carolini; Cantão, Maurício Egídio; Nogueira, Marco Antonio; Vasconcelos, Ana Tereza Ribeiro; Hungria, Mariangela.
Título: Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach
Fonte: Braz. j. microbiol;49(4):723-730, Oct.-Dec. 2018. graf.
Idioma: en.
Projeto: CNPq-Universal; . CNPq; . Embrapa.
Resumo: ABSTRACT The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspot for bioprospection of hydrolases.
Descritores: Solo/química
Proteínas Fúngicas/genética
Archaea/enzimologia
Proteínas Arqueais/genética
Fungos/enzimologia
Hidrolases/genética
-Microbiologia do Solo
Soja/crescimento & desenvolvimento
Triticum/crescimento & desenvolvimento
Brasil
Archaea/isolamento & purificação
Archaea/classificação
Archaea/genética
Zea mays/crescimento & desenvolvimento
Agricultura
Metagenoma
Metagenômica
Fungos/isolamento & purificação
Fungos/classificação
Fungos/genética
Responsável: BR1.1 - BIREME


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Id: biblio-974334
Autor: Hassan, Mariam; Essam, Tamer; Megahed, Salwa.
Título: Illumina sequencing and assessment of new cost-efficient protocol for metagenomic-DNA extraction from environmental water samples
Fonte: Braz. j. microbiol;49(supl.1):1-8, 2018. graf.
Idioma: en.
Resumo: Abstract In this study, the development and assessment of a modified, efficient, and cost-efficient protocol for mDNA (metagenomic DNA) extraction from contaminated water samples was attempted. The efficiency of the developed protocol was investigated in comparison to a well-established commercial kit (Epicentre, Metagenomic DNA Isolation Kit for Water). The comparison was in terms of degree of shearing, yield, purity, duration, suitability for polymerase chain reaction and next-generation sequencing in addition to the quality of next-generation sequencing data. The DNA yield obtained from the developed protocol was 2.6 folds higher than that of the commercial kit. No significant difference in the alpha (Observed species, Chao1, Simpson and PD whole tree) and beta diversity was found between the DNA samples extracted by the commercial kit and the developed protocol. The number of high-quality sequences of the samples extracted by the developed method was 20% higher than those obtained by the samples processed by the kit. The developed economic protocol successfully yielded high-quality pure mDNA compatible with complex molecular applications. Thus we propose the developed protocol as a gold standard for future metagenomic studies investigating a large number of samples.
Descritores: Bactérias/isolamento & purificação
DNA Bacteriano/isolamento & purificação
Métodos Analíticos de Preparação de Amostras/métodos
Metagenômica/economia
Metagenômica/métodos
Água Doce/microbiologia
-Filogenia
Bactérias/classificação
Bactérias/genética
DNA Bacteriano/genética
Análise de Sequência de DNA
Métodos Analíticos de Preparação de Amostras/economia
Água Doce/química
Responsável: BR1.1 - BIREME


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Id: biblio-828196
Autor: Medeiros, Julliane Dutra; Cantão, Maurício Egídio; Cesar, Dionéia Evangelista; Nicolás, Marisa Fabiana; Diniz, Cláudio Galuppo; Silva, Vânia Lúcia; Vasconcelos, Ana Tereza Ribeiro de; Coelho, Cíntia Marques.
Título: Comparative metagenome of a stream impacted by the urbanization phenomenon
Fonte: Braz. j. microbiol;47(4):835-845, Oct.-Dec. 2016. graf.
Idioma: en.
Resumo: Abstract Rivers and streams are important reservoirs of freshwater for human consumption. These ecosystems are threatened by increasing urbanization, because raw sewage discharged into them alters their nutrient content and may affect the composition of their microbial community. In the present study, we investigate the taxonomic and functional profile of the microbial community in an urban lotic environment. Samples of running water were collected at two points in the São Pedro stream: an upstream preserved and non-urbanized area, and a polluted urbanized area with discharged sewage. The metagenomic DNA was sequenced by pyrosequencing. Differences were observed in the community composition at the two sites. The non-urbanized area was overrepresented by genera of ubiquitous microbes that act in the maintenance of environments. In contrast, the urbanized metagenome was rich in genera pathogenic to humans. The functional profile indicated that the microbes act on the metabolism of methane, nitrogen and sulfur, especially in the urbanized area. It was also found that virulence/defense (antibiotic resistance and metal resistance) and stress response-related genes were disseminated in the urbanized environment. The structure of the microbial community was altered by uncontrolled anthropic interference, highlighting the selective pressure imposed by high loads of urban sewage discharged into freshwater environments.
Descritores: Urbanização
Microbiologia da Água
Rios/microbiologia
Metagenoma
Microbiota
-Filogenia
RNA Ribossômico 16S/genética
Ecossistema
Metabolismo Energético
Redes e Vias Metabólicas
Metagenômica
Código de Barras de DNA Taxonômico
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1017057
Autor: Rampadarath, Sillma; Bandhoa, Kushlata; Puchooa, Daneshwar; Jeewon, Rajesh; Bal, Subhasisa.
Título: Early bacterial biofilm colonizers in the coastal waters of Mauritius
Fonte: Electron. j. biotechnol;29:13-21, sept. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Mauritius Research Council for funding the project; . LANDRACE.
Resumo: Background: The past years have witnessed a growing number of researches in biofilm forming communities due to their environmental and maritime industrial implications. To gain a better understanding of the early bacterial biofilm community, microfiber nets were used as artificial substrates and incubated for a period of 24 h in Mauritian coastal waters. Next-generation sequencing technologies were employed as a tool for identification of early bacterial communities. Different genes associated with quorum sensing and cell motility were further investigated. Results: Proteobacteria were identified as the predominant bacterial microorganisms in the biofilm within the 24 h incubation, of which members affiliated to Gammaproteobacteria, Alphaproteobacteria and Betaproteobacteria were among the most abundant classes. The biofilm community patterns were also driven by phyla such as Firmicutes, Bacteroidetes, Chloroflexi, Actinobacteria and Verrucomicrobia. The functional analysis based on KEGG classification indicated high activities in carbohydrate, lipid and amino acids metabolism. Different genes encoding for luxI, lasI, agrC, flhA, cheA and cheB showed the involvement of microbial members in quorum sensing and cell motility. Conclusion: This study provides both an insight on the early bacterial biofilm forming community and the genes involved in quorum sensing and bacterial cell motility.
Descritores: Água do Mar/microbiologia
Bactérias/crescimento & desenvolvimento
Bactérias/genética
Fenômenos Fisiológicos Bacterianos
-Bactérias/isolamento & purificação
Bactérias/classificação
Aderência Bacteriana
Movimento Celular
Biofilmes
Biodiversidade
Percepção de Quorum
Incrustação Biológica
Metagenômica
Sequenciamento de Nucleotídeos em Larga Escala
Maurício
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-889237
Autor: Yasir, Muhammad.
Título: Analysis of bacterial communities and characterization of antimicrobial strains from cave microbiota
Fonte: Braz. j. microbiol;49(2):248-257, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah.
Resumo: Abstract In this study for the first-time microbial communities in the caves located in the mountain range of Hindu Kush were evaluated. The samples were analyzed using culture-independent (16S rRNA gene amplicon sequencing) and culture-dependent methods. The amplicon sequencing results revealed a broad taxonomic diversity, including 21 phyla and 20 candidate phyla. Proteobacteria were dominant in both caves, followed by Bacteroidetes, Actinobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, and the archaeal phylum Euryarchaeota. Representative operational taxonomic units from Koat Maqbari Ghaar and Smasse-Rawo Ghaar were grouped into 235 and 445 different genera, respectively. Comparative analysis of the cultured bacterial isolates revealed distinct bacterial taxonomic profiles in the studied caves dominated by Proteobacteria in Koat Maqbari Ghaar and Firmicutes in Smasse-Rawo Ghaar. Majority of those isolates were associated with the genera Pseudomonas and Bacillus. Thirty strains among the identified isolates from both caves showed antimicrobial activity. Overall, the present study gave insight into the great bacterial taxonomic diversity and antimicrobial potential of the isolates from the previously uncharacterized caves located in the world's highest mountains range in the Indian sub-continent.
Descritores: Bactérias/isolamento & purificação
Bactérias/classificação
Microbiologia Ambiental
Biota
Antibiose
-Paquistão
Filogenia
Bactérias/crescimento & desenvolvimento
Bactérias/genética
DNA Bacteriano/genética
DNA Bacteriano/química
DNA Ribossômico/genética
DNA Ribossômico/química
RNA Ribossômico 16S/genética
Análise por Conglomerados
Análise de Sequência de DNA
Euryarchaeota/isolamento & purificação
Euryarchaeota/classificação
Euryarchaeota/crescimento & desenvolvimento
Euryarchaeota/genética
DNA Arqueal/genética
DNA Arqueal/química
Metagenômica
Responsável: BR1.1 - BIREME



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