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Pesquisa : H01.158.273.343.350.261 [Categoria DeCS]
Referências encontradas : 27 [refinar]
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  1 / 27 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-1040578
Autor: Laboratório de Genética Molecular de MicrorganismosConteville, Liliane C; Laboratório de FlavivírusFilippis, Ana Maria B de; Laboratório de FlavivírusNogueira, Rita Maria R; Laboratório de FlavivírusMendonça, Marcos César L de; Laboratório de Genética Molecular de MicrorganismosVicente, Ana Carolina P.
Título: Metagenomic analysis reveals Hepatitis A virus in suspected yellow fever cases in Brazil
Fonte: Mem. Inst. Oswaldo Cruz;113(1):66-67, Jan. 2018. graf.
Idioma: en.
Projeto: CNPq; . FAPERJ.
Resumo: Using a metagenomic approach, we identified hepatitis A virus among cases of acute febrile illnesses that occurred in 2008-2012 in Brazil suspected as yellow fever. These findings reinforce the challenge facing routine clinical diagnosis in complex epidemiological scenarios.
Descritores: Febre Amarela/diagnóstico
Hepatite A/diagnóstico
-Febre Amarela/epidemiologia
Vírus da Febre Amarela/genética
Brasil/epidemiologia
Metagenômica
Genótipo
Hepatite A/epidemiologia
Vírus de Hepatite/genética
Limites: Humanos
Responsável: BR1.1 - BIREME


  2 / 27 LILACS  
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Texto completo SciELO Uruguai
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Id: lil-758740
Autor: Papone, Virginia; Verolo, Carolina; Zaffaroni, Lourdes; Batlle, Alicia; Capó, Claudia; Bueno, Luis; Gamonal, Jorge; Silva, Nora; Soria, Sandra.
Título: Detección y prevalencia de patógenos periodontales de una población con periodontitis crónica en Uruguay mediante metodología convencional y metagenómica / Detection and prevalence of periodontal pathogens in a Uruguayan population with chronic periodontitis using conventional methodology and metagenomics
Fonte: Odontoestomatol;17(25):23-33, mayo.2015.
Idioma: en; es.
Resumo: Las enfermedades periodontales son un significativo problema mundial a nivel de salud humana. Décadas de investigaciones, evidencian que en la mayoría de los casos la periodontitis crónica es la más común, caracterizada por ser de evolución lenta, con formación de bolsas periodontales, posterior reabsorción del hueso alveolar, pérdida y destrucción de piezas dentarias y tejido óseo. Si bien se reconoce el origen multifactorial en el desarrollo de la periodontitis, es relevante la participación de la microbiota subgingival en la etiología de la enfermedad periodontal. Algunas de las especies bacterianas patógenas que han sido asociadas con el desarrollo de la enfermedad periodontal son Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum, entre otras. En este estudio, nos propusimos investigar cuáles de éstas cinco especies estaban presentes en las bolsas periodontales de 51 pacientes uruguayos con periodontitis crónica. Para alcanzar éste objetivo se utilizó una técnica convencional microbiológica y metagenómica (multiplex-PCR). Los resultados de la técnica convencional microbiológica evidenciaron la presencia de A. actinomycetemcomitans (33%) y de bacterias negras pigmentadas anaerobias (100%) en las muestras. De los resultados obtenidos en la multiplex-PCR, se demostró que las especies de mayor prevalencia fueron F. nucleatum (100%), T. forsythia (92%) y P. gingivalis (88%). Por el contrario, las especies de menor prevalencia fueron P. intermedia (39%) y A. actinomycetemcomitans (33%)...

Periodontal diseases are a major problem in human health. Decades of research have shown that the most common disease is chronic periodontitis, characterized by a slow evolution with the formation of periodontal pockets, subsequent alveolar bone resorption, loss and destruction of teeth and bone tissue. While we know the multifactorial origin of the development of periodontitis, the participation of subgingival microbiota is relevant in the etiology of periodontal disease. Some pathogenic bacteria species that have been associated with the development of periodontal disease are Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum, among others. In this work we studied which of these five species were present in the periodontal pockets of 51 Uruguayan patients with chronic periodontitis. To achieve the results a conventional microbiological technique and metagenomics (multiplex-PCR) were used. The results of the microbiological conventional technique showed the presence of A. actinomycetemcomitans (33%) and black pigmented anaerobic bacteria (100%) in the samples. From the results obtained in the multiplex-PCR we saw that the most prevalent species were F. nucleatum (100%), T. forsythia (92%) and P. gingivalis (88%). In contrast, lower prevalence species were P. intermedia (39%) and A. actinomycetecomitans...(33%)
Descritores: Aggregatibacter actinomycetemcomitans
Fusobacterium nucleatum
Metagenômica
Periodontite Crônica/patologia
Porphyromonas gingivalis
Limites: Humanos
Responsável: UY20.1 - Departamento de Documentación y Biblioteca


  3 / 27 LILACS  
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Id: biblio-1292313
Autor: Bai, Ding-Ping; Lin, Xin-Yu; Hu, Yu-Qiong; Chen, Zhen-Zhen; Chen, Lu; Huang, Yi-Fan; Huang, Xiao-Hong; Li, Jian.
Título: Metagenomics approach to identify lignocellulose-degrading enzymes in the gut microbiota of the Chinese bamboo rat cecum
Fonte: Electron. j. biotechnol;50:29-36, Mar. 2021. tab, graf.
Idioma: en.
Projeto: Natural Science Foundation of Fujian Province of China; . Discipline Development Grant from the College of Animal Sciences FAFU.
Resumo: BACKGROUND: Lignocellulose is considered a renewable organic material, but the industrial production of biofuel from lignocellulose is challenging because of the lack of highly active hydrolytic enzymes. The guts of herbivores contain many symbiotic microorganisms that have evolved to hydrolyze plant lignocellulose. Chinese bamboo rats mainly consume high-fiber foods, indicating that some members of the intestinal tract microbiota digest lignocellulose, providing these rats with the energy required for growth. RESULTS: Here, we used metagenomics to analyze the diversity and functions of the gut microbiota in Chinese bamboo rats. We identified abundant populations of lignocellulose-degrading bacteria, whose main functions involved carbohydrate, amino acid, and nucleic acid metabolism. We also found 587 carbohydrate-active enzyme genes belonging to different families, including 7 carbohydrate esterase families and 21 glycoside hydrolase families. The glycoside hydrolase 3, glycoside hydrolase 1, glycoside hydrolase 43, carbohydrate esterase 4, carbohydrate esterase 1, and carbohydrate esterase 3 families demonstrated outstanding performance. CONCLUSIONS: The microbes and enzymes identified in our study expand the existing arsenal of proficient degraders and enzymes for lignocellulosic biofuel production. This study also describes a powerful approach for targeting gut microbes and enzymes in numerous industries.
Descritores: Ceco/enzimologia
Enzimas/metabolismo
Lignina/metabolismo
-Ceco/microbiologia
Celulose/metabolismo
Bacteroidetes
Biocombustíveis
Metagenômica
Firmicutes
Microbioma Gastrointestinal
Limites: Animais
Ratos
Responsável: CL1.1 - Biblioteca Central


  4 / 27 LILACS  
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Id: biblio-1022816
Autor: Infante, Claudia D; Castillo, Francisca; Pérez, Vilma; Riquelme, Carlos.
Título: Inhibition of Nitzschia ovalis biofilm settlement by a bacterial bioactive compound through alteration of EPS and epiphytic bacteria
Fonte: Electron. j. biotechnol;33:1-10, May. 2018. graf, tab, ilus.
Idioma: en.
Projeto: CONICYT; . Fondef IDeA; . University of Antofagasta.
Resumo: Background: Marine ecosystems contain benthic microalgae and bacterial species that are capable of secreting extracellular polymeric substances (EPS), suggesting that settlement of these microorganisms can occur on submerged surfaces, a key part of the first stage of biofouling. Currently, anti-fouling treatments that help control this phenomenon involve the use of biocides or antifouling paints that contain heavy metals, which over a long period of exposure can spread to the environment. The bacterium Alteromonas sp. Ni1-LEM has an inhibitory effect on the adhesion of Nitzschia ovalis, an abundant diatom found on submerged surfaces. Results: We evaluated the effect of the bioactive compound secreted by this bacterium on the EPS of biofilms and associated epiphytic bacteria. Three methods of EPS extraction were evaluated to determine the most appropriate and efficient methodology based on the presence of soluble EPS and the total protein and carbohydrate concentrations. Microalgae were cultured with the bacterial compound to evaluate its effect on EPS secretion and variations in its protein and carbohydrate concentrations. An effect of the bacterial supernatant on EPS was observed by assessing biofilm formation and changes in the concentration of proteins and carbohydrates present in the biofilm. Conclusions: These results indicate that a possible mechanism for regulating biofouling could be through alteration of biofilm EPS and alteration of the epiphytic bacterial community associated with the microalga.
Descritores: Diatomáceas
Biofilmes
Microalgas
Matriz Extracelular de Substâncias Poliméricas
-Ambiente Marinho
Incrustação Biológica
Metagenômica
Microbiota
Responsável: CL1.1 - Biblioteca Central


  5 / 27 LILACS  
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Id: biblio-1025039
Autor: Zeaiter, Zahraa; Mapelli, Francesca; Crotti, Elena; Borin, Sara.
Título: Methods for the genetic manipulation of marine bacteria
Fonte: Electron. j. biotechnol;33:17-28, May. 2018. ilus, tab.
Idioma: en.
Projeto: European Community's Seventh Framework Programme; . (MACUMBA); . EU Horizon 2020 Project.
Resumo: Genetic manipulation of bacteria is a procedure necessary to obtain new strains that express peculiar and defined genetic determinants or to introduce genetic variants responsible for phenotypic modifications. This procedure can be applied to explore the biotechnological potential associated with environmental bacteria and to utilize the functional properties of specific genes when inserted into an appropriate host. In the past years, marine bacteria have received increasing attention because they represent a fascinating reservoir of genetic and functional diversity that can be utilized to fuel the bioeconomy sector. However, there is an urgent need for an in-depth investigation and improvement of the genetic manipulation tools applicable to marine strains because of the paucity of knowledge regarding this. This review aims to describe the genetic manipulation methods hitherto used in marine bacteria, thus highlighting the limiting factors of the different techniques available today to increase manipulation efficiency. In particular, we focus on methods of natural and artificial transformations (especially electroporation) and conjugation because they have been successfully applied to several marine strains. Finally, we emphasize that, to avoid failure, future work should be carried out to establish tailored methodologies for marine bacteria.
Descritores: Água do Mar/microbiologia
Bactérias/genética
Engenharia Genética
-Transformação Bacteriana
Genoma
Eletroporação
Conjugação Genética
Metagenômica
Análise de Célula Única
Vetores Genéticos
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


  6 / 27 LILACS  
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Id: biblio-1177412
Autor: Gupta, Verruchi; Singh, Inderpal; Rasool, Shafaq; Verma, Vijeshwar.
Título: Next generation sequencing and microbiome's taxonomical characterization of frozen soil of north western Himalayas of Jammu and Kashmir, India
Fonte: Electron. j. biotechnol;45:30-37, May 15, 2020. ilus, graf.
Idioma: es.
Projeto: Department of Biotechnology, Govt. of India.
Resumo: BACKGROUND: Traditionally, microbial genome sequencing has been restrained to the species grown in pure culture. The development of culture-independent techniques over the last decade allows scientists to sequence microbial communities directly from environmental samples. Metagenomics is the study of complex genome by the isolation of DNA of the whole community. Next generation sequencing (NGS) of metagenomic DNA gives information about the microbial and taxonomical characterization of a particular niche. The objective of the present research is to study the microbial and taxonomical characterization of the metagenomic DNA, isolated from the frozen soil sample of a glacier in the north western Himalayas through NGS. RESULTS: The glacier community comprised of 16 phyla with the representation of members belonging to Proteobacteria and Acidobacteria. The number of genes annotated through the Kyoto Encyclopedia of Genes and Genomes (KEGG), GO, Pfam, Clusters of Orthologous Groups of proteins (COGs), and FIG databases were generated by COGNIZER. The annotation of genes assigned in each group from the metagenomics data through COG database and the number of genes annotated in different pathways through KEGG database were reported. CONCLUSION: Results indicate that the glacier soil taken in the present study, harbors taxonomically and metabolically diverse communities. The major bacterial group present in the niche is Proteobacteria followed by Acidobacteria, and Actinobacteria, etc. Different genes were annotated through COG and KEGG databases that integrate genomic, chemical, and systemic functional information.
Descritores: Microbiologia do Solo
Bactérias/classificação
Sequenciamento de Nucleotídeos em Larga Escala
Microbiota/genética
-Bactérias/isolamento & purificação
Clima Frio
Biologia Computacional
Camada de Gelo
Metagenômica
Genoma Microbiano
Índia
Responsável: CL1.1 - Biblioteca Central


  7 / 27 LILACS  
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Id: biblio-876987
Autor: Araújo, Paula Gonçalves de; Abreu, Camila Siriero Melo; Donato, Ludmila; Almeida, Juliano; Crippa, Mauro; Dumont, Alexandra; Corsi, Érica; Santos, Ronaldo Carneiro dos.
Título: Efeito de uma associação de cepas probióticas contendo lactobacillus e bifidobacterium na modulação da microbiota intestinal em pacientes constipados / Effects of association of probiotic strains containing lactobacillus and bifidobacterium on modulation of the intestinal microbiota in constipated patients
Fonte: GED gastroenterol. endosc. dig;36(3):89-98, Jul.-Set. 2017. ilus, tab.
Idioma: pt.
Resumo: Introdução: a constipação é uma doença crônica que afeta a população mundial, podendo ser consequência do desequilíbrio da microbiota intestinal, conhecida como disbiose. Nesse contexto, os probióticos vêm sendo utilizados com o objetivo de promover o seu equilíbrio, a fim de mantê-la saudável (eubiose). Objetivo: avaliar, em um estudo duplo-cego, randomizado, placebo-controlado, se o uso de uma associação de cepas probióticas contendo lactobacillus e bifidobacterium por 28 dias pode modular a microbiota intestinal em pacientes constipados. Método: a atividade da associação de cepas probióticas foi comparada com placebo (maltodextrina) após a suplementação por 28 dias. Os pacientes foram avaliados por meio da análise de metagenômica e da melhora dos sintomas relacionados à constipação. Os dados do perfil da população de microrganismos presentes no intestino dos pacientes foram correlacionados com os resultados da avaliação dos sintomas abdominais e da percepção de bem-estar geral. O aumento do número de evacuações e a melhora do trânsito intestinal foram avaliados durante o estudo. Resultados: as proporções do gênero Bifidobacterium no grupo teste e controle foram 0.45% vs 0.24% no final do estudo, respectivamente (p<0,05). Adicionalmente, a diferença entre os grupos que receberam associação de cepas probióticas contendo lactobacillus e bifidobacterium e placebo permaneceu também significativamente alta com relação às espécies de Lactobacillus (1,21% vs 0,12%) após 28 dias de tratamento (p<0,05). Além disso, os participantes que receberam o probiótico apresentaram uma tendência de melhora sintomática baseada na comparação da sua microbiota e as respostas oriundas da avaliação dos sintomas abdominais e bem-estar geral. Nenhum evento adverso grave foi relatado. Conclusão: a formulação probiótica modulou a microbiota intestinal de forma diferente do placebo nos participantes do estudo. O consumo dos probióticos aumentou significativamente as bactérias benéficas e reduziu as potencialmente maléficas, contribuindo para o equilíbrio da microbiota intestinal.

Introduction: constipation is a chronic disease that affects the world population, being a consequence of the imbalance of the intestinal microbiota, known as dysbiosis. In light of this context, the probiotics have been used to trigger microbiota intestinal balance, in order to keep it healthy (eubiose) Aim: to evaluate in a double-blind, randomized, placebo-controlled study whether the use of association of probiotic strains containing lactobacillus and bifidobacterium for 28 days can modulate the intestinal microbiota of the constipated participants. Methods: the activity of association of probiotic strains was compared to placebo (maltodextrin) after 28 days of consumption. The patients were evaluated by using metagenomics analyses and improved constipation symptoms. The dates of microorganisms population profile into patients gut were correlated with results of abdominal symptoms evaluation and well-being perception. The increase in the number of evacuations and the intestinal transit were evaluated during the study. Results: the proportions of the genus Bifidobacterium in the test groups and the control group were 0.45% vs 0.24% at the end of the study, respectively (p<0.05). Additionally, the difference between the groups that received association of probiotic strains containing lactobacillus and bifidobacterium and the placebo remained higher for species of the genus Lactobacillus (1.21% vs 0.12%) after 28 days of use (p<0.05). Furthermore, the participants who received the probiotic had a tendency to improve symptomatic based on comparisons of their microbiota and the responses provided patients' abdominal symptoms and well-being. There were no reports of serious adverse events during the study. Conclusion: the probiotic formulation modulated the intestinal microbiota differently from the placebo in constipated participants included in this study. The use of this probiotic significantly increased beneficial bacteria and decreased potentially harmful microbes, which contributed to the maintenance of a healthy intestinal microbiota.
Descritores: Bifidobacterium
Constipação Intestinal
Probióticos
Probióticos/farmacologia
Microbioma Gastrointestinal
Microbioma Gastrointestinal/efeitos dos fármacos
Lactobacillus
-Método Duplo-Cego
Metagenômica
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Tipo de Publ: Ensaio Clínico Controlado Aleatório
Responsável: BR9.1 - Biblioteca de Ciências da Saúde Profa. Susana Schimidt


  8 / 27 LILACS  
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Id: biblio-1087172
Autor: Salam, Menaka; Varma, Ajit.
Título: Bacterial community structure in soils contaminated with electronic waste pollutants from Delhi NCR, India
Fonte: Electron. j. biotechnol;41:72-80, sept. 2019. ilus, tab, graf.
Idioma: en.
Projeto: DST-SERB, Government of India, Young Scientist Scheme.
Resumo: Background: Microbial community analysis of electronic waste (e-waste)-polluted environments is of interest to understand the effect of toxic e-waste pollutants on the soil microbial community and to evaluate novel microorganisms resisting the toxic environment. The present study aims to investigate the bacterial community structure in soils contaminated with e-waste from various sites of Loni and Mandoli (National Capital Region (NCR), India) where e-waste dumping and recycling activities are being carried out for many years. Results: Interferences to soil metagenomic DNA extraction and PCR amplification were observed because of the presence of inhibiting components derived from circuit boards. Whole-metagenome sequencing on the Illumina MiSeq platform showed that the most abundant phyla were Proteobacteria and Firmicutes. Deltaproteobacteria and Betaproteobacteria were the most common classes under Proteobacteria. Denaturing gradient gel electrophoresis (DGGE) analysis of the bacterial 16S rRNA gene showed that e-waste contamination altered the soil bacterial composition and diversity. There was a decrease in the number of predominant bacterial groups like Proteobacteria and Firmicutes but emergence of Actinobacteria in the contaminated soil samples. Conclusions: This is the first report describing the bacterial community structure of composite soil samples of ewaste-contaminated sites of Loni and Mandoli, Delhi NCR, India. The findings indicate that novel bacteria with potential bioremediating properties may be present in the e-waste-contaminated sites and hence need to be evaluated further.
Descritores: Microbiologia do Solo
Bactérias/isolamento & purificação
Bactérias/genética
Resíduo Eletrônico/análise
-Poluentes do Solo
Reação em Cadeia da Polimerase
Metais Pesados
Proteobactérias/isolamento & purificação
Metagenômica
Eletroforese em Gel de Gradiente Desnaturante
Microbiota
Firmicutes/isolamento & purificação
Índia
Responsável: CL1.1 - Biblioteca Central


  9 / 27 LILACS  
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Id: biblio-1116917
Autor: Uberos, José.
Título: Microbiota perinatal: Revisión de su importancia en la salud del recién nacido / Perinatal microbiota: review of its importance in newborn health
Fonte: Arch. argent. pediatr;118(3):e265-e270, jun. 2020. ilus.
Idioma: en; es.
Resumo: La metagenómica en el estudio de los ecosistemas bacterianos intestinales ha permitido definir un perfil genético funcional estándar en el recién nacido, de forma que un ecosistema bacteriano será tanto más "normal" cuanto más se parezca su perfil genético funcional a un estándar. El desarrollo de determinado enterotipo funcional en los primeros días de vida tras el parto es fundamental para que tenga lugar el cebado del sistema inmunológico con determinados antígenos bacterianos. Independientemente de si las primeras bacterias intestinales se adquieren antes o justo después del parto, la microbiota del recién nacido va a ser el resultado de una situación de simbiosis con la flora microbiana de su entorno, en especial, con la flora bacteriana de su madre. El tipo de parto, la administración de antibióticos perinatales, el entorno y la exposición nutricional, en especial, la lactancia materna, han demostrado relacionarse de forma importante con el microbioma intestinal predominante

The use of metagenomics in the study of gut bacterial ecosystems has helped to define a standard, functional genetic profile in newborn infants, so that a bacterial ecosystem will be deemed more "normal" the more similar its functional genetic profile is to a standard. The development of a specific functional enterotype in the first days of life after birth is critical for the priming of the immune system with certain bacterial antigens.Regardless of whether the first gut bacteria are acquired before or just after birth, the newborn microbiota will result from the symbiosis with the environmental microbial flora, especially with the bacterial flora of the mother. The type of delivery, the administration of perinatal antibiotics, the environment, and nutritional exposure, especially breastfeeding, have demonstrated an important relationship with the prevalent gut microbiome
Descritores: Microbiota
-Bactérias
Aleitamento Materno
Parto
Metagenômica
Microbioma Gastrointestinal
Saúde do Lactente
Limites: Humanos
Masculino
Feminino
Recém-Nascido
Tipo de Publ: Revisão
Responsável: AR94.1 - Centro de Información Pediatrica


  10 / 27 LILACS  
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Id: lil-728528
Autor: Gutiérrez-Escobar, Andrés Julián; Bayona-Rojas, Martín; Barragan-Vidal, Carlos; Rojas-Lara, Sebastián; Oliveros, Ricardo.
Título: Metagenomic analysis of the gastric microbiota cultivable from a patient with gastritis concomitant with Barrett´s esophagus / Análisis metagenómico de la microbiota gástrica cultivable a partir de un paciente con gastritis concomitante con esófago de Barrett
Fonte: Rev. gastroenterol. Perú;34(3):229-235, jul. 2014. ilus, tab.
Idioma: en.
Resumo: Barrett’s esophagus is a distal metaplasia characterized by the transformation of squamous mucosa into columnar mucosa. This esophageal phenotype is a product not only of the chronic reflux of gastric acids, but also by microorganisms that colonize the oral cavity and stomach. Two classes of microbiota can be identified in Barrett’s esophagus; microbiota type I is associated with the normal esophagus and type II with an inflamed esophagus. The present study describes the gastric microbiota of a patient with antral gastritis concomitant with Barrett’s esophagus absent infection with Helicobacter pylori. Gastric biopsies were obtained following the protocol of Sydney and following ethical practices. The isolates were cultivated under microaerophilic conditions on Columbia Agar supplemented with IsoVitaleX™ and 7% sterile blood. Extracted DNA was sequenced using 454-GS and the results analyzed on the MG-RAST server. Gram negative isolates were found and bacteria resistant to levofloxacin, amoxicillin, tetracycline, erythromycin, and clarithromycin. The phyla Bacteroidetes, Firmicutes, Fusobacteria and Proteobacteria, the genus Bacteroides and the species group Bacteroides fragilis were most abundant. Functionally, the metabolism of carbohydrates, amino acids, and to a lesser extent, the metabolism of cofactors and vitamins were most dominant, and of which the enzymes β-glucosidase (EC 3.2.1.21), β-galactosidase (EC 3.2.1.23) and β-N-acetylhexosaminidase (EC 3.2.1.52) were most dominant. The findings of this study, because they are of only one case may probably suggest a possible pathogenic role, previously undescribed for Bacteroides fragilis, associated with human gastritis when concomitant esophageal pathology exists.

El esófago de Barrett es una metaplasia distal caracterizada por la transformación de la mucosa escamosa a mucosa columnar. Este fenotipo esofágico es producto no solo de la exposición crónica al reflujo de ácidos gástricos sino también a microbios colonizantes de la cavidad oral y del estómago. El esófago Barrett presenta 2 clases de microbiotas; la microbiota tipo I asociada con esófago normal y la tipo II a fenotipos esofágicos inflamatorios. En el presente estudio se describió la microbiota gástrica de una paciente con gastritis antral concomitante con esófago de Barrett sin infección por Helicobacter pylori y se obtuvieron biopsias gástricas siguiendo el protocolo de Sydney y estándares bioéticos. Los cultivos se hicieron en condiciones microaerofílicas en agar Columbia suplementados con isovitalex y sangre estéril al 7%. El ADN extraído fue sometido a secuenciación empleando 454 GS y las lecturas fueron analizadas en el servidor MG-RAST. Se obtuvieron aislamientos gram-negativos y resistentes a levofloxacina, amoxicilina, tetraciclina, eritromicina y claritromicina. Los Phylum Bacteroidetes, Firmicutes, Fusobacteria y Proteobacteria, el género Bacteroides y las especies de grupo Bacteroides fragilis fueron los más abundantes. Funcionalmente, el metabolismo de carbohidratos, aminoácidos y en menor grado el metabolismo de cofactores y vitaminas fueron los más dominantes; de los cuales las enzimas la β-glicosidasa (EC 3.2.1.21), β-galactosidasa (EC 3.2.1.23) y la β-N-acetilhexosaminidasa (EC 3.2.1.52) fueron las más dominantes. Estos resultados, por ser de un solo caso, solo podrían sugerir un posible papel patogénico no descrito para Bacterioides fragilis asociado con gastritis humana cuando existe patología esofágica concomitante.
Descritores: Esôfago de Barrett/microbiologia
Gastrite/microbiologia
Microbioma Gastrointestinal/genética
Metagenômica
Estômago/microbiologia
-Esôfago de Barrett/complicações
Gastrite/complicações
Limites: Feminino
Humanos
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: PE1.1 - Oficina Universitária de Biblioteca



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