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Pesquisa : H01.158.273.540.274.555 [Categoria DeCS]
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Id: biblio-1177412
Autor: Gupta, Verruchi; Singh, Inderpal; Rasool, Shafaq; Verma, Vijeshwar.
Título: Next generation sequencing and microbiome's taxonomical characterization of frozen soil of north western Himalayas of Jammu and Kashmir, India
Fonte: Electron. j. biotechnol;45:30-37, May 15, 2020. ilus, graf.
Idioma: es.
Projeto: Department of Biotechnology, Govt. of India.
Resumo: BACKGROUND: Traditionally, microbial genome sequencing has been restrained to the species grown in pure culture. The development of culture-independent techniques over the last decade allows scientists to sequence microbial communities directly from environmental samples. Metagenomics is the study of complex genome by the isolation of DNA of the whole community. Next generation sequencing (NGS) of metagenomic DNA gives information about the microbial and taxonomical characterization of a particular niche. The objective of the present research is to study the microbial and taxonomical characterization of the metagenomic DNA, isolated from the frozen soil sample of a glacier in the north western Himalayas through NGS. RESULTS: The glacier community comprised of 16 phyla with the representation of members belonging to Proteobacteria and Acidobacteria. The number of genes annotated through the Kyoto Encyclopedia of Genes and Genomes (KEGG), GO, Pfam, Clusters of Orthologous Groups of proteins (COGs), and FIG databases were generated by COGNIZER. The annotation of genes assigned in each group from the metagenomics data through COG database and the number of genes annotated in different pathways through KEGG database were reported. CONCLUSION: Results indicate that the glacier soil taken in the present study, harbors taxonomically and metabolically diverse communities. The major bacterial group present in the niche is Proteobacteria followed by Acidobacteria, and Actinobacteria, etc. Different genes were annotated through COG and KEGG databases that integrate genomic, chemical, and systemic functional information.
Descritores: Microbiologia do Solo
Bactérias/classificação
Sequenciamento de Nucleotídeos em Larga Escala
Microbiota/genética
-Bactérias/isolamento & purificação
Clima Frio
Biologia Computacional
Camada de Gelo
Metagenômica
Genoma Microbiano
Índia
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1223238
Autor: Li, Xianjun; Wang, Junhuan; Wu, Wei; Jia, Yang; Fan, Shuanghu; Su Hlaing, Thet; Khokhar, Ibatsam; Yan, Yanchun.
Título: Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase
Fonte: Electron. j. biotechnol;46:38-49, jul. 2020. ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Basic Research Fund of Chinese Academy of Agricultural Sciences.
Resumo: BACKGROUND: Quizalofop-p-ethyl (QPE), a unitary R configuration aromatic oxyphenoxypropionic acid ester (AOPP) herbicide, was widely used and had led to detrimental environmental effects. For finding the QPEdegrading bacteria and promoting the biodegradation of QPE, a series of studies were carried out. RESULTS: A QPE-degrading bacterial strain YC-XJ1 was isolated from desert soil and identified as Methylobacterium populi, which could degrade QPE with methanol by cometabolism. Ninety-seven percent of QPE (50 mg/L) could be degraded within 72 h under optimum biodegradation condition of 35°C and pH 8.0. The maximum degradation rate of QPE was 1.4 mg/L/h, and the strain YC-XJ1 exhibited some certain salinity tolerance. Two novel metabolites, 2-hydroxy-6-chloroquinoxaline and quinoxaline, were found by high-performance liquid chromatography/mass spectroscopy analysis. The metabolic pathway of QPE was predicted. The catalytic efficiency of strain YC-XJ1 toward different AOPPs herbicides in descending order was as follows: haloxyfop-pmethyl ≈ diclofop-methyl ≈ fluazifop-p-butyl N clodinafop-propargyl N cyhalofop-butyl N quizalofop-p-ethyl N fenoxaprop-p-ethyl N propaquizafop N quizalofop-p-tefuryl. The genome of strain YC-XJ1 was sequenced using a combination of PacBio RS II and Illumina platforms. According to the annotation result, one α/ß hydrolase gene was selected and named qpeh1, for which QPE-degrading function has obtained validation. Based on the phylogenetic analysis and multiple sequence alignment with other QPE-degrading esterases reported previously, the QPEH1 was clustered with esterase family V. CONCLUSION: M. populi YC-XJ1 could degrade QPE with a novel pathway, and the qpeh1 gene was identified as one of QPE-degrading esterase gene.
Descritores: Propionatos/metabolismo
Quinoxalinas/metabolismo
Methylobacterium/metabolismo
-Microbiologia do Solo
Biodegradação Ambiental
Methylobacterium/enzimologia
Methylobacterium/genética
Análise de Sequência de Proteína
Esterases/análise
Esterases/metabolismo
Herbicidas
Hidrolases/análise
Hidrolases/metabolismo
Hidrólise
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950822
Autor: Aftab, Usman; Zechel, David L; Sajid, Imran.
Título: Antitumor compounds from Streptomyces sp. KML-2, isolated from Khewra salt mines, Pakistan
Fonte: Biol. Res;48:1-10, 2015. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Actinomycetes are gram positive bacteria with high G + C content in their DNA and are capable of producing variety of secondary metabolites. Many of these metabolites possess different biological activities and have the potential to be developed as therapeutic agents. The aim of the present study was to screen actinomycetes inhabiting halophilic environment such as Khewra salt mines present in Pakistan for cytotoxic and antitumor compounds. RESULTS: An actiomycetes strain designated as Streptomyces sp. KML-2 was isolated from a saline soil of Khewra salt mines, Pakistan. The strain Streptomyces sp. KML-2 showed 84 % cytotoxic activity against larvae of Artemiasalina. In the screening phase, the strain exhibited significant antitumor activity with IC50 values of 12, 48 and 56 µg/ml against Hela, MDBK and Vero cell lines, respectively. After that extract from 20 l fermentation was used to purify secondary metabolites by several chromatographic techniques. Structure elucidation of isolated compounds revealed that it is highly stable producer of Chromomycin SA (1) and 1-(1H-indol-3-yl)-propane-1,2,3-triol (2). Both of the isolated compounds showed significant antitumor activity against Hela and MCF-7 cancer cell lines (IC50 values 8.9 and 7.8 µg/ml against Hela; 12.6 and 0.97 µg/ml against MCF-7, respectively). The 16S rRNA gene sequence (1437 bp) of the strain confirm its identity (99 %) with Streptomyces griseus. CONCLUSIONS: From this research work we were successful in isolating two potent antitumor compounds, Chromomycin SA and 1-(1H-indol-3-yl)-propane-1,2,3-triol from Streptomyces KML-2 strain, isolated from Khewra salt mine. As such this is the second report which confirms that S. griseus can produce Chromomycin SA without introducing any mutagenesis in its biosynthesizing gene cluster and isolated indole derivative is being reported first time from any member of actinomycetes group with having novel antitumor activity against Hela and MCF-7 cells Nucleotide sequences: Nucleotide sequence data reported are available in the GenBank database under the accession number: GenBank KJ009562.
Descritores: Microbiologia do Solo
Streptomyces/química
Antineoplásicos/farmacologia
-Paquistão
Filogenia
Artemia/classificação
Artemia/efeitos dos fármacos
Sais
Solo/química
Streptomyces/isolamento & purificação
Streptomyces/ultraestrutura
Streptomyces griseus/classificação
Sais de Tetrazólio
Células Vero
RNA Ribossômico 16S/genética
Cromomicinas/classificação
Cromomicinas/farmacologia
Células HeLa
Microscopia Eletrônica de Varredura
Linhagem Celular
Chlorocebus aethiops
Cromatografia/métodos
Análise de Sequência de RNA
Concentração Inibidora 50
Células MCF-7
Formazans
Glicerol/análogos & derivados
Glicerol/farmacologia
Larva/efeitos dos fármacos
Mineração
Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Antineoplásicos/isolamento & purificação
Limites: Humanos
Animais
Bovinos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1124216
Autor: Vásquez-Dean, Javiera; Maza, Felipe; Morel, Isidora; Pulgar, Rodrigo; González, Mauricio.
Título: Microbial communities from arid environments on a global scale: a systematic review
Fonte: Biol. Res;53:29, 2020. graf.
Idioma: en.
Projeto: FONDAP; . FONDECYT.
Resumo: Abstract Arid environments are defined by the lack of water availability, which is directly related to the mean annual precipitation (MAP), and high values of solar irradiation, which impacts the community composition of animals, plants, and the microbial structure of the soil. Recent advances in NGS technologies have expanded our ability to characterize micro- biomes, allowing environmental microbiologists to explore the complete microbial structure. Intending to identify and describe the state-of-the-art of bacterial communities in arid soils at a global scale, and to address the effect that some environmental features may have on them, we performed a systematic review based on the PRISMA guideline. Using a combination of keywords, we identified a collection of 66 studies, including 327 sampled sites, reporting the arid soil bacterial community composition by 16S rDNA gene high-throughput sequencing. To identify factors that can modulate bacterial communities, we extracted the geographical, environmental, and physicochemical data. The results indicate that even though each sampled site was catalogued as arid, they show wide variability in altitude, mean annual temperature (MAT), soil pH and electric conductivity, within and between arid environments. We show that arid soils display a higher abundance of Actinobacteria and lower abundance of Proteobacteria, Cyanobacteria, and Planctomycetes, compared with non-arid soil microbiomes, revealing that microbial structure seems to be strongly modulated by MAP and MAT and not by pH in arid soils. We observed that environmental and physicochemical features were scarcely described among studies, hence, we propose a reporting guideline for further analysis, which will allow deepening the knowledge of the relationship between the microbiome and abiotic factors in arid soil. Finally, to understand the academic collaborations landscape, we developed an analysis of the author's network, corroborating a low degree of connectivity and collaborations in this research topic. Considering that it is crucial to understand how microbial processes develop and change in arid soils, our analysis emphasizes the need to increase collaborations between research groups worldwide.
Descritores: Microbiologia do Solo
Microbiota/genética
-Plantas
Solo
Proteobactérias
Limites: Animais
Tipo de Publ: Revisão Sistemática
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1132266
Autor: Tančić-Živanov, Sonja; Medić-Pap, Slađana; Danojević, Dario; Prvulović, Dejan.
Título: Effect of Trichoderma spp. on Growth Promotion and Antioxidative Activity of Pepper Seedlings
Fonte: Braz. arch. biol. technol;63:e20180659, 2020. tab, graf.
Idioma: en.
Projeto: Ministry of Education, Science and Technological Development of the Republic of Serbia.
Resumo: Abstract One of the main challenges in pepper production is to enhance seed germination energy and germination, and to grow healthy nursery plants with strong root system. Trichoderma species colonize roots as they grow and provide season-long benefits to plants, which is why Trichoderma species are widely used as plant growth promoter agents and promoters of plant defence mechanisms. This study evaluated the effectiveness of seed biopriming with Trichoderma isolates for growth promotion of pepper plants in early stage and their effects on seedling physiology. Nine out of ten Trichoderma isolates positively affected root weight of pepper seedlings, while three out of ten positively affected shoot weight. Root and shoot lengths were mainly unaffected. Germination energy was positively affected by five isolates with up to 40% increase compared to the control, while germination was significantly enhanced by two isolates with up to 22% increase. Considering seedling physiology, two different strain-dependent modes of actions were expressed. Promising Trichoderma isolates induced formation and accumulation of reactive oxygen species (ROS) which acted as signal molecules that increased germination energy and germination. Positive correlation was found between pyrogallol peroxidase, superoxide dismutase, catalase activity and germination in plants treated with these isolates.
Descritores: Microbiologia do Solo
Trichoderma/metabolismo
Germinação/fisiologia
Gênero Pimenta/crescimento & desenvolvimento
-Espécies Reativas de Oxigênio/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-1142512
Autor: Rosa, Graciele Ferreira da; Casali, Carlos Alberto; Bechara, Fernando Campanha; Viceli, Jéssica Maiara; Moreira, Flavia Lima; Guimarães, Bruna Schneider; Ferreira, André Francisco; Peppe, Isabella Araújo; Acosta, Amanda Cristina Beal; Dôres, Letícia de Alcântara; Feix, Bruna Larissa.
Título: Soil Microbiological Attributes Under Ecological Restoration Technologies in Subtropical Forest
Fonte: Braz. arch. biol. technol;63(spe):e20190651, 2020. tab.
Idioma: en.
Resumo: Abstract To accelerate the recovery of degraded environments, it is necessary to use ecological restoration techniques, which require validation according to the ecosystem conditions where are implemented. This work aimed to evaluate soil microbiological attributes under different ecological restoration technologies in a subtropical forest. The study was conducted at UTFPR-DV, southwest of Paraná, in an ecotone between Mixed Ombrophilous Forest and Semideciduous Seasonal Forest and on an Oxisol. In December 2010, a tillage area of at least 17 years old was isolated and the passive restoration, tree planting and nucleation treatments were installed in 40x54 m plots and four replications. In November 2018 the soil was sampled in these plots and in a native forest area as a reference. There were calculated soil organic carbon content (OCC) and microbiological attributes such as microbial biomass N and C (NMIC and CMIC), basal respiration, fungal spore content and the metabolic (qCO2) and microbial quotient (MICq). It can be concluded that nucleation technology can restore soil microbiological attributes but has not yet reached the conditions of a natural environment. Passive restoration is not a good technology for restoring soil microbiological attributes. The higher contents of CMIC, NMIC, OCC, MICq and fungal spores in the soil under native forest compared to ecological restoration technologies indicate that eight years of adoption of these techniques have not yet been enough to fully recover soil microbiological activity.
Descritores: Microbiologia do Solo
Florestas
Conservação dos Recursos Naturais/métodos
-Esporos Fúngicos
Clima Tropical
Brasil
Carbazóis
Carbono/análise
Nitrogênio/análise
Responsável: BR1.1 - BIREME


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Id: biblio-1142514
Autor: Antunes, Carlos Henrique; Etto, Rafael Mazer; Caires, Eduardo Fávero; Bini, Angelo Rafael; Gonçalves, Daniel Ruiz Potma; Urrea-Valencia, Salomé; Galvão, Carolina Weigert.
Título: Liming and Nitrogen Fertilization Effects on Soil Microbial Community in Long Term No-till
Fonte: Braz. arch. biol. technol;63(spe):e20190492, 2020. tab, graf.
Idioma: en.
Resumo: Abstract Soil management influences organic matter decomposition rates as well soil microbial community functional behavior. No-till (NT) is the most used management system by farmers due to its conservation practices and high productivity. The main objective of this study was to evaluate the impact of surface-applied lime, nitrogen (N) application, and black oat residues on soil microbial community of a Typic Hapludox under continuous NT. Therefore, soil chemical attributes, microbial biomass carbon, basal respiration, metabolic quotient, most probable number of diazotrophs, as well as bacterial functional analysis were performed. The effect of liming and N fertilization amendments inputs were saw in soil respiration and metabolic quotient measurements, showing them to be good indicators of soil quality. Further studies should be carried out in order to molecularly identify microbial communities present in soils with different liming and N fertilization management to evaluate the behavior of specific bacterial taxa under such conditions.
Descritores: Solo/química
Microbiologia do Solo
Qualidade do Solo
Compostos de Cálcio/administração & dosagem
Fertilizantes
Nitrogênio/administração & dosagem
-Análise do Solo
Microbiota
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1171793
Autor: Barrera Viviana A; Kageyama Koji; Rojo Rodrigo A; Gasoni Laura; Kobayashi Kiroku.
Título: A species-specific method for detecting pathogenic Streptomyces species from soil and potato tubers in Argentina / A species-specific method for detecting pathogenic Streptomyces species from soil and potato tubers in Argentina.
Fonte: Rev. argent. microbiol;45(4):277-81, dic. 2013.
Idioma: es.
Resumo: Potato common scab is caused by several soil-inhabiting pathogenic Streptomyces species. In the present study, a species-specific PCR method was used to detect Streptomyces species in potato tuber lesions and soils. Total genomic DNA from soil samples from six locations and tuber samples from four potato cultivars (Spunta, Shepody, Innovator and Russet Burbank) were assessed. Streptomyces scabies, Streptomyces acidiscabies, and Streptomyces turgidiscabies were detected in soybean, tobacco and potato soils and in all potato varieties except Russet Burbank. The phylogenetic analysis of the sequences obtained confirmed the identification. The method proposed proved to be time-saving and cost effective for the rapid detection of Streptomyces species. This is the first report of the detection of S. acidiscabies and S. turgidiscabies in soils and potato tubers from Argentina.
Descritores: Doenças das Plantas/microbiologia
Microbiologia do Solo
Solanum tuberosum/microbiologia
Streptomyces/isolamento & purificação
-Argentina
Especificidade da Espécie
Técnicas Bacteriológicas/métodos
Tipo de Publ: Artigo de Revista
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


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Id: biblio-1147257
Autor: Schapovaloff, María E; Angeli Alves, Luis F; Urrutia, María I; López Lastra, Claudia C.
Título: Ocurrencia natural de hongos entomopatógenos en suelos cultivados con yerba mate (Ilex paraguariensis St. Hil.) en Misiones, Argentina / Natural ocurrence of entomopathogenic fungi in soils cultivated with Paraguay tea (Ilex paraguariensis St. Hil.) in Misiones, Argentina
Fonte: Rev. argent. microbiol;47(2):138-142, June 2015.
Idioma: es.
Resumo: Este trabajo tuvo por objetivo aislar, identificar y caracterizar morfológicamente hongos entomopatógenos presentes en suelos cultivados con yerba mate (Ilex paraguariensis St. Hil.). Se realizó una prospección de hongos entomopatógenos nativos a partir de 40 muestras de suelos cultivados con yerba mate ubicados en la provincia de Misiones, República Argentina, desde mayo de 2008 hasta junio de 2010. Las muestras de suelo se obtuvieron en cercanías de plantas de yerba mate, se aplicó la técnica de dilución seriada en medios de cultivos selectivos. Después de obtenidos los cultivos puros, los hongos fueron caracterizados macroscópica y microscópicamente, y se los identificó mediante el uso de claves taxonómicas específicas. Se aislaron 29 cepas fúngicas pertenecientes a las especies Beauveria bassiana (n = 17), Metarhizium anisopliae (n = 2) y Purpureocillium lilacinum (n = 10)

This study aimed to morphologically isolate, identify and characterize entomopathogenic fungi present in soils cultivated with Paraguay tea (Ilex paraguariensis). A survey of native entomopathogenic fungi was conducted from 40 soil samples grown with Paraguay tea in the province of Misiones, Argentina, from May 2008 to June 2010. The soil dilution plate methodology on selective culture media was used to isolate microorganisms. Taxonomic identification was performed using macroscopic and microscopic characters and specific keys. Twenty nine strains, belonging to the species Beauveria bassiana (n = 17), Metarhizium anisopliae (n = 2) and Purpureocillium lilacinum (n = 10) were isolated and identified
Descritores: Ilex paraguariensis
Ilex paraguariensis/microbiologia
Beauveria/isolamento & purificação
Metarhizium/isolamento & purificação
Fungos/isolamento & purificação
Fungos/classificação
-Microbiologia do Solo
Tipo de Publ: Estudo de Avaliação
Estudo Observacional
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


  10 / 331 LILACS  
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Id: biblio-1122177
Autor: Silva, Jefté Teixeira da; Menezes, Keli Pinheiro; Melchior, Leonardo Augusto Kohara; Nunes, Vânia Lúcia Brandão; Brilhante, Andreia Fernandes.
Título: Occurrence of geohelminths in the soil of public squares in Rio Branco, Acre State, Brazilian Western Amazon / Ocorrência de geohelmintos em solo de praças públicas em Rio Branco, estado do Acre, Amazônia Brasileira
Fonte: Braz. J. Vet. Res. Anim. Sci. (Online);57(2):e163783, mai. 2020. tab.
Idioma: en.
Resumo: Soil contamination by dog and cat feces can become a public health problem due to the transmission of various etiologic agents that cause zoonoses. This study aimed to verify the occurrence of geohelminths in the soil of some public square areas of the municipality of Rio Branco, Acre State, Western Brazilian Amazon. Five public squares were selected, and soil samples collection was performed from April 2014 to March 2015. The samples were processed by using the Baermann-Moraes and centrifugal-flotation methods. Geohelminths positivity was of 25% for Tox o c ara spp., 6.6% for the Ancylostomatoidea Superfamily and 1.6 for Trichuris spp.. Measures should be implemented to prevent the free access of animals to these places, as well as deworming of stray dogs and cats and the implementation of population control and policies for such animals.(AU)

A contaminação do solo por fezes de cães e gatos pode se tornar um problema de saúde pública devido à transmissão de vários agentes etiológicos que causam zoonoses. Este estudo objetivou verificar a ocorrência de geohelmintos no solo de praças públicas do município de Rio Branco, estado do Acre, Brasil. Cinco praças públicas foram selecionadas e amostras de solo foram colhidas entre abril de 2014 e março de 2015. As amostras foram processadas pelos métodos de Baermann-Moraes e centrífugo-flutuação. A positividade observada para geohelmintos foi de 25% para Tox o c ara spp., 6,6% para a Superfamília Ancylostomatoidea e 1,6% para Trichuris spp.. Medidas devem ser implementadas para prevenir a livre circulação de animais nestes locais, bem como a desverminação de cães e gatos de rua e a implementação de políticas de adoção e controle populacional de cães e gatos.(AU)
Descritores: Microbiologia do Solo
Toxocara/isolamento & purificação
Ancylostomatoidea/isolamento & purificação
Poluição Ambiental
-Brasil
Zoonoses
Transmissão de Doença Infecciosa
Ecossistema Amazônico
Jardins
Limites: Animais
Gatos
Cães
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice



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