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Pesquisa : H01.158.273.540.460 [Categoria DeCS]
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Id: biblio-949378
Autor: Maia, Guilherme Tavares da Silva; Albuquerque, Amanda Vasconcelos de; Martins Filho, Euclides Dias; Lira Neto, Filipe Tenório de; Souza, Veridiana Sales Barbosa de; Silva, Anderson Arnaldo da; Lira, Mariana Montenegro de Melo; Lima, Salvador Vilar Correia.
Título: Bacterial cellulose to reinforce urethrovesical anastomosis. A translational study
Fonte: Acta cir. bras;33(8):673-683, Aug. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate the efficacy of the cellulosic exopolysaccharide membrane (CEM) as a urethral reinforcement for urethrovesical anastomosis. Methods: Twenty eight rabbits were submitted to urethrovesical anastomosis with or without CEM reinforcement. The animals were divided into 4 groups: C7, CEM7, C14 and CEM14: (C= only anastomosis or CEM = anastomosis + CEM), evaluated after 7 weeks, and 14 weeks. The biointegration and biocompatibility of CEM were evaluated according to stenosis, fistula, urethral wall thickness, urethral epithelium, rate of inflammation and vascularization. Results: Between the two experimental groups, the difference in the number of stenosis or urinary fistula was not statistically significant. The morphometric analysis revealed preservation of urethral lumen, well adhered CEM without extrusion, a controlled inflammatory process and implant vascularization. The urothelium height remained constant over time after CEM reinforcement and the membrane wall was thicker, statistically, after 14 weeks. Conclusion: The absence of extrusion, stenosis or urinary fistula after 14 weeks of urethrovesical anastomosis demonstrates cellulosic exopolysaccharide membrane biocompatibility and biointegration with tendency to a thicker wall.
Descritores: Uretra/cirurgia
Materiais Biocompatíveis/uso terapêutico
Bexiga Urinária/cirurgia
Celulose/uso terapêutico
-Polissacarídeos Bacterianos/uso terapêutico
Fatores de Tempo
Uretra/patologia
Bexiga Urinária/patologia
Microbiologia Industrial/métodos
Teste de Materiais
Anastomose Cirúrgica
Celulose/biossíntese
Reprodutibilidade dos Testes
Resultado do Tratamento
Pesquisa Médica Translacional
Neovascularização Patológica
Limites: Animais
Masculino
Coelhos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  2 / 89 LILACS  
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Id: biblio-951792
Autor: Moghannem, Saad A. M; Farag, Mohamed M. S; Shehab, Amr M; Azab, Mohamed S.
Título: Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
Fonte: Braz. j. microbiol;49(3):452-462, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett-Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters. Box-Behnken RSM indicated that; the optimum concentration for each parameter was 12% (w/v) for molasses, 6 g/L yeast extract and 30 °C for incubation temperature. The most potent bacterial isolate was identified as Bacillus velezensis KY498625. After production, EPS was extracted, purified using DEAE-cellulose, identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and gas chromatography-mass spectroscopy (GC-MS). The result indicated that; it has molecular weight 1.14 × 105 D consisting of glucose, mannose and galactose.
Descritores: Polissacarídeos Bacterianos/metabolismo
Bacillus/metabolismo
-Polissacarídeos Bacterianos/isolamento & purificação
Polissacarídeos Bacterianos/química
Bacillus/química
Microbiologia Industrial
Espectroscopia de Infravermelho com Transformada de Fourier
Meios de Cultura/metabolismo
Meios de Cultura/química
Peso Molecular
Responsável: BR1.1 - BIREME


  3 / 89 LILACS  
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Id: biblio-974324
Autor: Revin, Victor; Liyaskina, Elena; Nazarkina, Maria; Bogatyreva, Alena; Shchankin, Mikhail.
Título: Cost-effective production of bacterial cellulose using acidic food industry by-products
Fonte: Braz. j. microbiol;49(supl.1):151-159, 2018. tab, graf.
Idioma: en.
Resumo: Abstract To reduce the cost of obtaining bacterial cellulose, acidic by-products of the alcohol and dairy industries were used without any pretreatment or addition of other nitrogen sources. Studies have shown that the greatest accumulation of bacterial cellulose (6.19 g/L) occurs on wheat thin stillage for 3 days of cultivation under dynamic conditions, which is almost 3 times higher than on standard Hestrin and Schramm medium (2.14 g/L). The use of whey as a nutrient medium makes it possible to obtain 5.45 g/L bacterial cellulose under similar conditions of cultivation. It is established that the pH of the medium during the growth of Gluconacetobacter sucrofermentans B-11267 depends on the feedstock used and its initial value. By culturing the bacterium on thin stillage and whey, there is a decrease in the acidity of the waste. It is shown that the infrared spectra of bacterial cellulose obtained in a variety of environments have a similar character, but we found differences in the micromorphology and crystallinity of the resulting biopolymer.
Descritores: Resíduos/análise
Microbiologia Industrial/métodos
Celulose/biossíntese
Gluconacetobacter/metabolismo
-Resíduos/economia
Triticum/metabolismo
Triticum/microbiologia
Microbiologia Industrial/economia
Indústria Alimentícia
Meios de Cultura/economia
Meios de Cultura/metabolismo
Gluconacetobacter/crescimento & desenvolvimento
Etanol/metabolismo
Responsável: BR1.1 - BIREME


  4 / 89 LILACS  
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Id: biblio-974317
Autor: Zepeda, Andrea B; Pessoa Jr, Adalberto; Farías, Jorge G.
Título: Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast
Fonte: Braz. j. microbiol;49(supl.1):119-127, 2018. tab, graf.
Idioma: en.
Projeto: Comisión Nacional de Investigación Científica y Tecnológica de Chile; . AZP; . FAPESP.
Resumo: Abstract Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others; but to control all of them most of the time is difficult and this depends on the initial selection of each variable. Therefore, this review focuses on the selection of the best promoter in the recombination process, considering different inductors, and the temperature as a culture medium variable in methylotrophic Pichia pastoris yeast. The goal is to understand the effects associated with different factors that influence its cell metabolism and to reach the construction of an expression system that fulfills the requirements of the yeast, presenting an optimal growth and development in batch, fed-batch or continuous cultures, and at the same time improve its yield in heterologous protein production.
Descritores: Pichia/genética
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/genética
Carbono/metabolismo
Regiões Promotoras Genéticas
-Pichia/crescimento & desenvolvimento
Pichia/metabolismo
Temperatura
Microbiologia Industrial
Responsável: BR1.1 - BIREME


  5 / 89 LILACS  
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Id: biblio-974316
Autor: Reis, Cristiane Bianchi Loureiro dos; Morandini, Liziane Maria Barassuol; Bevilacqua, Caroline Borges; Bublitz, Fabricio; Ugalde, Gustavo; Mazutti, Marcio Antonio; Jacques, Rodrigo Josemar Seminoti.
Título: First report of the production of a potent biosurfactant with α, ß-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation
Fonte: Braz. j. microbiol;49(supl.1):185-192, 2018. tab, graf.
Idioma: en.
Resumo: Abstract Biosurfactants have many advantages over synthetic surfactants but have higher production costs. Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost. The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule. The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi. To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used. The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose. The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry. F. fujikuroi reduced surface tension from 72 to 20 mN m−1 under the optimized conditions of pH 5.0, 37 °C and 7 days of incubation with 190 rpm agitation. The partial identification of the structure of the biosurfactant demonstrated the presence of an α,β-trehalose. The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.
Descritores: Tensoativos/metabolismo
Trealose/metabolismo
Microbiologia Industrial/métodos
Fusarium/metabolismo
-Tensoativos/química
Temperatura
Meios de Cultura/metabolismo
Fermentação
Fusarium/crescimento & desenvolvimento
Fusarium/química
Concentração de Íons de Hidrogênio
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  6 / 89 LILACS  
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Id: biblio-974343
Autor: Nuanpeng, Sunan; Thanonkeo, Sudarat; Klanrit, Preekamol; Thanonkeo, Pornthap.
Título: Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices
Fonte: Braz. j. microbiol;49(supl.1):140-150, 2018. tab, graf.
Idioma: en.
Resumo: Abstract Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed. As found in this study, an ALM with dimensions of 20 × 20 × 5 mm3 is effective for cell immobilization due to its compact structure and long-term stability. The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system. By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined. The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54 g/L and 1.36 g/L h, respectively. The use of the ALM-immobilized cells was successful for at least six consecutive batches (360 h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.
Descritores: Saccharomyces cerevisiae/metabolismo
Microbiologia Industrial/métodos
Sorghum/microbiologia
Etanol/metabolismo
-Saccharomyces cerevisiae/química
Células Imobilizadas/metabolismo
Células Imobilizadas/química
Sorghum/metabolismo
Sorghum/química
Etanol/análise
Alginatos/química
Fermentação
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-839329
Autor: Lopes, Mario Lucio; Paulillo, Silene Cristina de Lima; Godoy, Alexandre; Cherubin, Rudimar Antonio; Lorenzi, Marcel Salmeron; Giometti, Fernando Henrique Carvalho; Bernardino, Claudemir Domingos; Amorim Neto, Henrique Berbert de; Amorim, Henrique Vianna de.
Título: Ethanol production in Brazil: a bridge between science and industry
Fonte: Braz. j. microbiol;47(supl.1):64-76, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT In the last 40 years, several scientific and technological advances in microbiology of the fermentation have greatly contributed to evolution of the ethanol industry in Brazil. These contributions have increased our view and comprehension about fermentations in the first and, more recently, second-generation ethanol. Nowadays, new technologies are available to produce ethanol from sugarcane, corn and other feedstocks, reducing the off-season period. Better control of fermentation conditions can reduce the stress conditions for yeast cells and contamination by bacteria and wild yeasts. There are great research opportunities in production processes of the first-generation ethanol regarding high-value added products, cost reduction and selection of new industrial yeast strains that are more robust and customized for each distillery. New technologies have also focused on the reduction of vinasse volumes by increasing the ethanol concentrations in wine during fermentation. Moreover, conversion of sugarcane biomass into fermentable sugars for second-generation ethanol production is a promising alternative to meet future demands of biofuel production in the country. However, building a bridge between science and industry requires investments in research, development and transfer of new technologies to the industry as well as specialized personnel to deal with new technological challenges.
Descritores: Etanol
Fermentação
-Ciência
Tecnologia
Leveduras/metabolismo
Microbiologia Industrial
Brasil
Biocombustíveis
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-839324
Autor: Cachumba, Jorge Javier Muso; Antunes, Felipe Antonio Fernandes; Peres, Guilherme Fernando Dias; Brumano, Larissa Pereira; Santos, Júlio César Dos; Silva, Silvio Silvério Da.
Título: Current applications and different approaches for microbial L-asparaginase production
Fonte: Braz. j. microbiol;47(supl.1):77-85, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: ABSTRACT L-asparaginase (EC 3.5.1.1) is an enzyme that catalysis mainly the asparagine hydrolysis in L-aspartic acid and ammonium. This enzyme is presented in different organisms, such as microorganisms, vegetal, and some animals, including certain rodent's serum, but not unveiled in humans. It can be used as important chemotherapeutic agent for the treatment of a variety of lymphoproliferative disorders and lymphomas (particularly acute lymphoblastic leukemia (ALL) and Hodgkin's lymphoma), and has been a pivotal agent in chemotherapy protocols from around 30 years. Also, other important application is in food industry, by using the properties of this enzyme to reduce acrylamide levels in commercial fried foods, maintaining their characteristics (color, flavor, texture, security, etc.) Actually, L-asparaginase catalyzes the hydrolysis of L-asparagine, not allowing the reaction of reducing sugars with this aminoacid for the generation of acrylamide. Currently, production of L-asparaginase is mainly based in biotechnological production by using some bacteria. However, industrial production also needs research work aiming to obtain better production yields, as well as novel process by applying different microorganisms to increase the range of applications of the produced enzyme. Within this context, this mini-review presents L-asparaginase applications, production by different microorganisms and some limitations, current investigations, as well as some challenges to be achieved for profitable industrial production.
Descritores: Asparaginase/biossíntese
Microbiologia Industrial
Indústria Farmacêutica
Fermentação
Antineoplásicos
-Asparaginase
Indústria Alimentícia
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


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Id: lil-780837
Autor: Colla, Luciane Maria; Primaz, Andreiza Lazzarotto; Benedetti, Silvia; Loss, Raquel Aparecida; Lima, Marieli de; Reinehr, Christian Oliveira; Bertolin, Telma Elita; Costa, Jorge Alberto Vieira.
Título: Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi
Fonte: Braz. j. microbiol;47(2):461-467, Apr.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract A Plackett–Burman Factorial Design of 16 experiments was conducted to assess the influence of nine factors on the production of lipases by filamentous fungi. The factors investigated were bran type (used as the main carbon source), nitrogen source, nitrogen source concentration, inducer, inducer concentration, fungal strain (Aspergillus niger or Aspergillus flavus were selected as good lipase producers via submerged fermentation), pH and agitation. The concentration of the yeast extract and soybean oil and the pH had a significant effect (p < 0.05) on lipase production and were consecutively studied through a Full Factorial Design 23, with the concentration of yeast extract and pH being significant (p < 0.05). These variables were optimized using a central composite design, obtaining maximum lipolytic activities with the use of 45 g/L of yeast extract and pH 7.15. The statistical model showed a 94.12% correlation with the experimental data.
Descritores: Aspergillus flavus/metabolismo
Aspergillus niger/metabolismo
Microbiologia Industrial/métodos
Proteínas Fúngicas/biossíntese
Lipase/biossíntese
-Carbono/metabolismo
Meios de Cultura/metabolismo
Meios de Cultura/química
Fermentação
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  10 / 89 LILACS  
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Id: biblio-889166
Autor: Li, Yun-Cheng; Gou, Zi-Xi; Zhang, Ying; Xia, Zi-Yuan; Tang, Yue-Qin; Kida, Kenji.
Título: Inhibitor tolerance of a recombinant flocculating industrial Saccharomyces cerevisiae strain during glucose and xylose co-fermentation
Fonte: Braz. j. microbiol;48(4):791-800, Oct.-Dec. 2017. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Talent Project for Science and Technology Innovation of Sichuan Province.
Resumo: ABSTRACT Lignocellulose-derived inhibitors have negative effects on the ethanol fermentation capacity of Saccharomyces cerevisiae. In this study, the effects of eight typical inhibitors, including weak acids, furans, and phenols, on glucose and xylose co-fermentation of the recombinant xylose-fermenting flocculating industrial S. cerevisiae strain NAPX37 were evaluated by batch fermentation. Inhibition on glucose fermentation, not that on xylose fermentation, correlated with delayed cell growth. The weak acids and the phenols showed additive effects. The effect of inhibitors on glucose fermentation was as follows (from strongest to weakest): vanillin > phenol > syringaldehyde > 5-HMF > furfural > levulinic acid > acetic acid > formic acid. The effect of inhibitors on xylose fermentation was as follows (from strongest to weakest): phenol > vanillin > syringaldehyde > furfural > 5-HMF > formic acid > levulinic acid > acetic acid. The NAPX37 strain showed substantial tolerance to typical inhibitors and showed good fermentation characteristics, when a medium with inhibitor cocktail or rape straw hydrolysate was used. This research provides important clues for inhibitors tolerance of recombinant industrial xylose-fermenting S. cerevisiae.
Descritores: Saccharomyces cerevisiae/efeitos dos fármacos
Xilose/metabolismo
Glucose/metabolismo
-Fenóis/metabolismo
Fenóis/farmacologia
Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Ácidos/metabolismo
Ácidos/farmacologia
Microbiologia Industrial
Fermentação
Furanos/metabolismo
Furanos/farmacologia
Responsável: BR1.1 - BIREME



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