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Id: lil-522211
Autor: Rasmussen-Poblete, Susana; Valdes, Jorge; Gamboa, Maria Cecilia; Valenzuela, Pablo D. T; Krauskopf, Erwin.
Título: Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
Fonte: Electron. j. biotechnol;11(2):56-68, Apr. 2008. ilus, graf, tab.
Idioma: en.
Projeto: Universidad Andrés Bello; . MIFAB; . Microsoft Joint Research Program.
Resumo: Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3 percent of the contigs and 88.5 percent of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that provides an initial model for genes and regulatory pathways involved in cell wall biosynthesis at low temperature.
Descritores: Clima Frio
Biblioteca Gênica
Pinus taeda
Populus tremuloides
Indústria de Papel e Celulose
Responsável: CL1.1 - Biblioteca Central

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