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Id: biblio-1017082
Autor: Mei, Lanju; Dong, Na; Li, Fosheng; Li, Na; Yao, Min; Chen, Fang; Tang, Lin.
Título: Transcriptome analysis of female and male flower buds of Idesia polycarpa Maxim. var. vestita Diels
Fonte: Electron. j. biotechnol;29:39-46, sept. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Science and Technology support projects of Sichuan Province.
Resumo: Background: Idesia polycarpa Maxim. var. vestita Diels, a dioecious plant, is widely used for biodiesel due to the high oil content of its fruits. However, it is hard to distinguish its sex in the seedling stage, which makes breeding and production problematic as only the female tree can produce fruits, and the mechanisms underlying sex determination and differentiation remain unknown due to the lack of available genomic and transcriptomic information. To begin addressing this issue, we performed the transcriptome analysis of its female and male flower. Results: 28,668,977 and 22,227,992 clean reads were obtained from the female and male cDNA libraries, respectively. After quality checks and de novo assembly, a total of 84,213 unigenes with an average length of 1179 bp were generated and 65,972 unigenes (78.34%) could be matched in at least one of the NR, NT, Swiss-Prot, COG, KEGG and GO databases. Functional annotation of the unigenes uncovered diverse biological functions and processes, including reproduction and developmental process, which may play roles in sex determination and differentiation. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed many unigenes annotated as metabolic pathways, biosynthesis of secondary metabolites pathways, plant­ pathogen interaction, and plant hormone signal transduction. Moreover, 29,953 simple sequence repeats were identified using the microsatellite software. Conclusion: This work provides the first detailed transcriptome analysis of female and male flower of I. polycarpa and lays foundations for future studies on the molecular mechanisms underlying flower bud development of I. polycarpa.
Descritores: Reprodução/genética
Salicaceae/genética
Transcriptoma
-Análise de Sequência de RNA
Genes de Plantas
Repetições de Microssatélites
Salicaceae/crescimento & desenvolvimento
Bases de Dados Genéticas
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Cuba
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Id: lil-739227
Autor: Martínez Ortiz, Carlos M; Sautié Castellanos, Miguel; Cuza Ferrer, Yordanka; Wisdom Viña, Yinette.
Título: Herramienta web para la clasificación de microsatélites polimórficos en genomas bacterianos / Web Tool for classfication of polymorphic microsatellites in bacteria genomes
Fonte: Rev. cuba. inform. méd;5(1):20-29, ene.-jun. 2013.
Idioma: es.
Resumo: Las secuencias repetidas en tándem, específicamente los mini y micro satélites, han demostrado ser muy eficaces en la clasificación de bacterias patogénicas como B. anthracis, M. tuberculosis y P. aeruginosa, entre otras. En humanos es manifiesta su participación estando relacionados con más de ochenta enfermedades, gran parte de ellas de tipo neurodegenerativas, musculares y algunos tipos de cáncer. La herramienta web que presentamos es el resultado de la detección computacional de estas secuencias en genomas bacterianos completos y su correspondiente anotación en la estructura genómica de acuerdo a las diferentes regiones donde estos se localizan. La herramienta tiene como fin primario brindar un sistema relacional que permita al investigador ubicar los microsatélites de diferentes especies bacterianas, con más de un genoma secuenciado para inferir su posible carácter polimórfico, dentro del contexto de la estructura genómica y así proveer un primer acercamiento al rol putativo que los microsatélites desempeñan desde el punto de vista funcional. La herramienta se puede aplicar no solo en estudios taxonómicos y epidemiológicos sino en la detección de posibles relaciones de estas secuencias con las funciones moleculares, procesos biológicos y, en última instancia, las diversas formas de evolución de estas especies. El sitio web brinda el servicio de consultas a la base de datos de microsatélites bacterianos de acuerdo al sistema de tablas relacionales y atributos propios de las mismas. Cuenta además con los servicios típicos de un sitio con estas características como: sistema de autenticación, foro, encuestas, enlaces y documentación sobre la metodología empleada y del tema en cuestión(AU)

The tandem repeat sequences, especially mini and microsatellites, have proven to be very effective in classification of pathogenic bacteria such as B. anthracis, M. tuberculosis and P. aeruginosa, among others. In human beings it is manifest its participation, being related with over eighty diseases, nearly all neurodegenerative and muscular, and some kinds of cancer. The web tool we are offering here is the result of computational detection of these sequences in whole bacteria genomes, and its respective annotation in the genomic structure according to the different regions where they are localized. The primary goal of this tool is to offer a relational system that allows mapping the microsatellites of bacterial species, all of them with more than one genome sequenced to infer their possible polymorphic character, in the context of genomic structure and thus providing a first approach to the putative role they perform from the functional point of view. The tool can be applied not only in taxonomical and epidemiological studies but in the detection of possible relationships of these sequences with the molecular functions, the biological processes and, as a last resort, the different forms of these species evolution. The web site offers the service of queries to the bacterial microsatellites database according to the related tables and its inherent attributes. It also has the typical services of this kind of site like: logging system, forum, polls, links and documentation about the employed methodology and the topic(AU)
Descritores: Bactérias
Repetições de Microssatélites/genética
Sequências de Repetição em Tandem/genética
Bases de Dados Genéticas
Limites: Seres Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-906287
Autor: Bonini, Jéssica Augusti; Stringhini, Rômulo M; Facalde, Laís; Librelotto, Giovani R.
Título: Estendendo o domínio da Ontocancro 3. 0 para abordar o Inflammaging / Extending the field of Ontocancro 3. 0 to approach the Inflammaging
Fonte: J. health inform;8(supl.I):341-351, 2016. ilus, graf.
Idioma: pt.
Conferência: Apresentado em: Congresso Brasileiro de Informática em Saúde, 15, Goiânia, 27-30 nov. 2016.
Resumo: Esse trabalho tem o objetivo de integrar os dados das versões anteriores da Ontocancro, projeto que visa o desenvolvimento de estudos na área da genética, e as especificações do termo Inflammaging, o qual é definido pelo estado inflamatório crônico devido ao envelhecimento. A Ontocancro 3.0 possibilitou a disponibilização de uma maior quantidade de dados, importantes para a compreensão do processo celular do Câncer, Alzheimer e Diabetes tipo 2, doenças com possível origem no estado inflamatório.

That work aims to integrate data from previous versions of Ontocancro, a project that aims to develop studiesin genetics, and the specifications of Inflammaging term, which is defined by chronic inflammatory condition due toaging. The 3.0 Ontocancro allowed the provision of a larger amount of data, important for understanding the cellular process of cancer, Alzheimer's disease and type 2 diabetes, diseases with a possible rise in the inflammatory state.
Descritores: Biologia Computacional
Bases de Dados Genéticas
Ontologia Genética
-Congressos como Assunto
Limites: Seres Humanos
Responsável: BR21.1 - Biblioteca J Baeta Vianna- Campus Saúde UFMG


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Texto completo SciELO Brasil
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Id: biblio-890679
Autor: Yang, Guodong; Chen, Shuping; Ma, Aiqun; Lu, Jun; Wang, Tingzhong.
Título: Identification of the difference in the pathogenesis in heart failure arising from different etiologies using a microarray dataset
Fonte: Clinics;72(10):600-608, Oct. 2017. tab, graf.
Idioma: en.
Projeto: Science and Technology Program.
Resumo: OBJECTIVES: Clinically, patients with chronic heart failure arising from different etiologies receive the same treatment. However, the prognoses of these patients differ. The purpose of this study was to elucidate whether the pathogenesis of heart failure arising from different etiologies differs. METHODS: Heart failure-related dataset GSE1145 was obtained from the Gene Expression Omnibus database. Differentially expressed genes were identified using R. A protein-protein interaction network of the differentially expressed genes was constructed using Search Tool for the Retrieval of Interacting Genes. The modules in each network were analyzed by Molecular Complex Detection of Cytoscape. The Database for Annotation, Visualization and Integrated Discovery was used to obtain the functions of the modules. RESULTS: Samples contained in GSE1145 were myocardial tissues from patients with dilated cardiomyopathy, familial cardiomyopathy, hypertrophic cardiomyopathy, ischemic cardiomyopathy, and post-partum cardiomyopathy. The differentially expressed genes, modules, and functions of the modules associated with different etiologies varied. Abnormal formation of extracellular matrix was overlapping among five etiologies. The change in cytoskeleton organization was specifically detected in dilated cardiomyopathy. The activation of the Wnt receptor signaling pathway was limited to hypertrophic cardiomyopathy. The change in nucleosome and chromatin assembly was associated with only familial cardiomyopathy. Germ cell migration and disrupted cellular calcium ion homeostasis were solely detected in ischemic cardiomyopathy. The change in the metabolic process of glucose and triglyceride was detected in only post-partum cardiomyopathy. CONCLUSION: These results indicate that the pathogenesis of heart failure arising from different etiologies varies, which may provide molecular evidence supporting etiology-based treatment for heart failure patients.
Descritores: Cardiomiopatias/genética
Expressão Gênica
Insuficiência Cardíaca/genética
-Cardiomiopatias/complicações
Estudos de Casos e Controles
Bases de Dados Genéticas
Perfilação da Expressão Gênica
Regulação da Expressão Gênica
Insuficiência Cardíaca/terapia
Análise em Microsséries
Mapas de Interação de Proteínas
Valores de Referência
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-848121
Autor: Souza, Paulo Vinicius Sporleder de.
Título: Biobancos, dados genéticos e proteção jurídico-penal da intimidade / Biobanks, genetic data and legal and criminal protection of privacy
Fonte: Rev. AMRIGS;56(3):68-73, jul.-set. 2012.
Idioma: pt.
Resumo: O artigo trata da questão dos biobancos para pesquisa e dos aspectos jurídicos relacionados à proteção de dados genéticos dos sujeitos de pesquisa. Mais especificamente, além da definição de biobancos e dados genéticos, analisa-se os dados genéticos e testes genéticos e a relação médico-sujeito de pesquisa, bem como a proteção criminal da intimidade genética, sugerindo-se a criação de uma lei que regulamente a coleta, o acesso, a utilização e o armazenamento dos dados genéticos a fim de prevenir testes genéticos para fins médicos arbitrários (AU)

The article deals with the issue of biobanks for research and legal aspects related to the protection of genetic data of study subjects. More specifically, besides the definition of biobanks and genetic data, we analyze genetic data and genetic testing and the physician-subject relationship in research, as well as criminal protection of genetic privacy, suggesting the creation of a law that regulates the collection, access, use and storage of genetic data to prevent genetic testing for arbitrary medical purposes (AU)
Descritores: Bases de Dados Genéticas/legislação & jurisprudência
Privacidade Genética/legislação & jurisprudência
Testes Genéticos/legislação & jurisprudência
Limites: Seres Humanos
Responsável: BR18.1 - Biblioteca FAMED/HCPA


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Id: biblio-843501
Autor: Barajas-Martinez, Hector; Hu, Dan; Baranchuk, Adrian.
Título: Genetics bases of cardiac sodium channel mutations linked to inherited cardiac arrhythmias / Bases genéticas de mutações de canal de sódio cardíaco ligadas a arritmias cardíacas herdadas / Bases genéticas de las mutaciones del canal de sodio cardíaco relacionadas con arritmias cardiacas hereditarias
Fonte: Rev. bras. crescimento desenvolv. hum;26(3):277-279, 2016.
Idioma: en.
Descritores: Arritmias Cardíacas/congênito
Arritmias Cardíacas/diagnóstico
Canalopatias
Bases de Dados Genéticas
Canais de Sódio/genética
-Hereditariedade
Fenótipo
Limites: Seres Humanos
Masculino
Feminino
Responsável: BR85.1 - Biblioteca Dante Moreira Leite


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Texto completo SciELO Chile
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Id: lil-774430
Autor: Jin, Ying; Zhao, Chunzhe; Chen, Lihui; Liu, Xiangyu; Pan, Shuxiao; Ju, Dongsheng; Ma, Jing; Li, Jinying; Wei, Bo.
Título: Identification of novel gene and pathway targets for human epilepsy treatment
Fonte: Biol. Res;49:1-9, 2016. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: The aim of this study was to explore epilepsy-related mechanism so as to figure out the possible targets for epilepsy treatment. METHODS: The gene expression profile dataset GES32534 was downloaded from Gene Expression Omnibus database. We identified the differentially expressed genes (DEGs) by Affy package. Then the DEGs were used to perform gene ontology (GO) and pathway enrichment analyses. Furthermore, a protein-protein interaction (PPI) network was constructed with the DEGs followed by co-expression modules construction and analysis. RESULTS: Total 420 DEGs were screened out, including 214 up-regulated and 206 down-regulated genes. Functional enrichment analysis revealed that down-regulated genes were mainly involved in the process of immunity regulation and biological repairing process while up-regulated genes were closely related to transporter activity. PPI network analysis showed the top ten genes with high degrees were all down-regulated, among which FN1 had the highest degree. The up-regulated and down-regulated DEGs in the PPI network generated two obvious sub-co-expression modules, respectively. In up-co-expression module, SCN3B (sodium channel, voltage gated, type III beta subunit) was enriched in GO:0006814 ~ sodium ion transport. In down-co-expression module, C1QB (complement C1s), CIS (complement component 1, S subcomponent) and CFI (complement factor I) were enriched in GO:0006955 ~ immune response. CONCLUSION: The immune response and complement system play a major role in the pathogenesis of epilepsy. Additionally, C1QB, C1S, CFI, SCN3B and FN1 may be potential therapeutic targets for epilepsy.
Descritores: Epilepsia/genética
Epilepsia/terapia
Perfilação da Expressão Gênica/métodos
Transcriptoma
-Bases de Dados Genéticas
Regulação para Baixo
Ontologia Genética
Redes Reguladoras de Genes
Marcação de Genes
Mapas de Interação de Proteínas
Regulação para Cima
Limites: Seres Humanos
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: lil-769828
Autor: d’Avila-Levy, Claudia Masini; Boucinha, Carolina; Kostygov, Alexei; Santos, Helena Lúcia Carneiro; Morelli, Karina Alessandra; Grybchuk-Ieremenko, Anastasiia; Duval, Linda; Votýpka, Jan; Yurchenko, Vyacheslav; Grellier, Philippe; Lukeš, Julius.
Título: Exploring the environmental diversity of kinetoplastid flagellates in the high-throughput DNA sequencing era
Fonte: Mem. Inst. Oswaldo Cruz;110(8):956-965, Dec. 2015. graf.
Idioma: en.
Projeto: FP7; . Bioglobe; . Moravskoslezský Kraj; . Czech Science Foundation; . Czech Science Foundation.
Resumo: The class Kinetoplastea encompasses both free-living and parasitic species from a wide range of hosts. Several representatives of this group are responsible for severe human diseases and for economic losses in agriculture and livestock. While this group encompasses over 30 genera, most of the available information has been derived from the vertebrate pathogenic genera Leishmaniaand Trypanosoma. Recent studies of the previously neglected groups of Kinetoplastea indicated that the actual diversity is much higher than previously thought. This article discusses the known segment of kinetoplastid diversity and how gene-directed Sanger sequencing and next-generation sequencing methods can help to deepen our knowledge of these interesting protists.
Descritores: Biodiversidade
DNA de Protozoário/genética
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Kinetoplastida/genética
Filogenia
RNA de Protozoário/genética
-Biomarcadores
Biologia Computacional
Bases de Dados Genéticas
Código de Barras de DNA Taxonômico/tendências
Meio Ambiente
Kinetoplastida/classificação
Kinetoplastida/citologia
Metagenômica/tendências
/genética
RNA, RIBOSOMAL, 1ABDOMINAL NEOPLASMSS/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-748310
Autor: Galego, Pedro; Silva, Fernando C.; Pinheiro, Luís Campos.
Título: Analysis of monotherapy prostate brachytherapy in patients with prostate cancer. Initial PSA and Gleason are important for recurrence?
Fonte: Int. braz. j. urol;41(2):353-359, Mar-Apr/2015. tab, graf.
Idioma: en.
Resumo: Purpose To evaluate the clinical outcome of a cohort of localized prostate cancer patients treate with 125-I permanent brachytherapy at the São José Hospital – CHLC, Lisbon. Materials and Methods A retrospective analysis was carried out on 429 patients with low and intermediate-risk of prostate adenocarcinoma, according to the recommendations of the EORTC, who underwent 125I brachytherapies in intraoperative dosimetry “real-time” system between September 2003 and September 2013. Results The mean follow-up was 71.98 months. Biochemical relapse of disease by rising PSA (Phoenix criterion) was observed in 18 patients (4.2%). Through the application of Kaplan-Meier survival curves in this sample, the rate of survival at 6 years without biochemical relapse was higher than 95%. By Iog rank test comparing biochemical relapse with initial PSA (15-10 and <10) and Gleason values (7 and <7), there was no statistical difference (P=0.830) of the initial PSA in the probability of developing biochemical relapse. In relation to Gleason score, it was noted a statistical difference (P<0.05), demonstrating that patients with Gleason 7 are more likely to develop biochemical relapse. Conclusions Brachytherapy as monotherapy is at present an effective choice in the treatment of localized prostate adenocarcinoma. Biochemical relapses are minimal. The initial PSA showed no statistically difference in the rate of relapses, unlike the value Gleason, where it was demonstrated that patients with Gleason 7 have a higher probability of biochemical relapse. Cases with PSA bounce should be controlled before starting a salvage treatment. .
Descritores: Genoma Humano
Estudo de Associação Genômica Ampla
Loci Gênicos/genética
Pneumopatias/genética
Capacidade Vital/genética
-Estudos de Coortes
Bases de Dados Genéticas
Seguimentos
Volume Expiratório Forçado
Predisposição Genética para Doença
Pneumopatias/patologia
Metanálise como Assunto
Prognóstico
Polimorfismo de Nucleotídeo Único/genética
Locos de Características Quantitativas/genética
Testes de Função Respiratória
Espirometria
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-742756
Autor: PINTO, Andressa dos Santos; GRIGOLETTI, Shana Souza; MARCADENTI, Aline.
Título: Fasting abbreviation among patients submitted to oncologic surgery: systematic review / Abreviação do jejum entre pacientes submetidos à cirurgia oncológica: revisão sistemática
Fonte: ABCD arq. bras. cir. dig;28(1):70-73, 2015. tab, graf.
Idioma: en.
Resumo: INTRODUCTION: The abbreviation of perioperative fasting among candidates to elective surgery have been associated with shorter hospital stay and decreased postoperative complications. OBJECTIVE: To conduct a systematic review from randomized controlled trials to detect whether the abbreviation of fasting is beneficial to patients undergoing cancer surgery compared to traditional fasting protocols. METHOD: A literature search was performed in electronic databases: MEDLINE (PubMed), SciELO, EMBASE and Cochrane, without time restriction. Were used the descriptors: "preoperative fasting", "cancer", "diet restriction" and "perioperative period". Randomized trials were included in adults of both sexes, with diagnosis of cancer. Exclusion criteria were: use of parenteral nutrition and publications in duplicate. All analyzes, selections and data extraction were done blinded manner by independent evaluators. RESULTS: Four studies were included, with a total of 150 patients, 128 with colorectal cancer and 22 gastric cancer. The articles were published from 2006 to 2013. The main outcome measures were heterogeneous, which impaired the unification of the results by means of meta-analysis. Compared to traditional protocols, patients undergoing fasting abbreviation with the administration of fluids containing carbohydrates had improvements in glycemic parameters (fasting glucose and insulin resistance), inflammatory markers (interleukin 6 and 10) and indicators of malnutrition (grip strength hand and CRP/albumin ratio), and shorter hospital stay. The methodological quality of the reviewed articles, however, suggests that the results should be interpreted with caution. CONCLUSION: The abbreviation of perioperative fasting in patients with neoplasm appears to be beneficial. .

INTRODUÇÃO: A abreviação do jejum perioperatório em pacientes candidatos à operações eletivas associa-se com menor tempo de internação hospitalar e diminuição de complicações pós-operatórias. OBJETIVO: Conduzir uma revisão sistemática a partir de ensaios clínicos randomizados controlados para detectar se a abreviação do jejum traz benefícios para indivíduos submetidos à cirurgia oncológica comparativamente aos protocolos de jejum tradicionais. MÉTODO: A busca na literatura foi realizada nas bases de dados eletrônicas: MEDLINE (Pubmed), Scielo, EMBASE e Cochrane, sem restrição de período. Utilizaram-se os descritores: "preoperative fasting", "cancer", "diet restriction" e "perioperative period". Foram incluídos ensaios clínicos randomizados, em indivíduos adultos, de ambos os sexos, com diagnóstico de câncer. Consideraram-se critérios de exclusão: uso de nutrição parenteral e publicações em duplicata. Todas as análises, seleções e extração dos dados foram feitas de maneira cega por avaliadores de forma independente. RESULTADOS: Foram incluídos quatro artigos, com total de 150 pacientes, sendo 128 com câncer colorretal e 22 câncer gástrico. Os artigos foram publicados no período de 2006 a 2013. Os desfechos principais foram heterogênios, o que impediu a unificação dos resultados por meio de metanálise. Comparativamente aos protocolos tradicionais, os indivíduos submetidos à abreviação do jejum com a administração de líquidos contendo carboidratos tiveram melhora nos parâmetros glicêmicos (glicemia de jejum e resistência a insulina), inflamatórios (interleucina 6 e 10) e nos marcadores de desnutrição (força do aperto de mão e razão PCR/albumina), assim como menor tempo de internação. A qualidade metodológica dos artigos avaliados, porém, sugere que os resultados sejam interpretados com cautela. CONCLUSÃO: A abreviação do jejum perioperatório em pacientes com neoplasias parece ser benéfica. .
Descritores: Doença de Crohn/genética
/genética
INTERLEUKIN-ABETALIPOPROTEINEMIA/genética
/genética
INTERLEUKIN-ABORTIFACIENT AGENTS, NONSTEROIDAL/genética
-Bases de Dados Genéticas
Predisposição Genética para Doença
Estudo de Associação Genômica Ampla
Polimorfismo de Nucleotídeo Único
Transdução de Sinais
Limites: Seres Humanos
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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