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Id: lil-731975
Autor: Martín Díaz, Ihomer; Fuster Callaba, Carlos; French Pacheco, Leidys; Marquetti Fernández, María del Carmen.
Título: Vigilancia entomológica con énfasis en aedes aegypti (Díptera: Culicidae) en campos de desplazados en Haití, 2010-2011 / Entomological surveillance with an emphasis on aedes aegypti (Díptera: Culicidae) in displaced persons camps of Haiti, 2010-2011
Fonte: Rev. cuba. med. trop;66(2):228-240, Mayo.-ago. 2014. Ilus.
Idioma: es.
Resumo: INTRODUCCIÓN: el terremoto en Haití en enero del 2010, condujo al agravamiento de las condiciones higiénicas, sanitarias y ambientales de un gran número de personas sin viviendas. OBJETIVO: evaluar la vigilancia vectorial con énfasis sobre Aedes aegypti, en un campo de desplazados post terremoto en la comuna de Carrefour en Haití, Junio 2010-Junio 2011. MÉTODOS: se determinaron la presencia y la densidad de Ae. aegypti, Anopheles albimanus y Culex quinquefasciatus por medio de encuestas larvales. Se emplearon también medidas de control adulticidas y uso de larvicidas para Ae. aegypti. RESULTADOS: se identificaron 7 especies de mosquitos. Los valores obtenidos de los índices casa, depósito y Breteau evidenció el mantenimiento de las densidades de Ae. aegypti con un incremento en la segunda mitad del año. Los depósitos misceláneos constituyeron el mayor por ciento de criaderos con 56 % del total, seguido por los neumáticos con 27 %. An. albimanus y Cx. quinquefasciatus mostraron escasa presencia en el área estudiada. Los tratamientos adulticidas contribuyeron a controlar las densidades de mosquitos en las instituciones con presencia cubana y en las áreas del campo de desplazados. CONCLUSIONES: durante el período estudiado se mantuvo una vigilancia y control constante sobre los mosquitos contribuyendo a que no se reportaran enfermedades transmitidas por estos vectores en el campo de desplazados.

INTRODUCTION: as a result of the earthquake occurring in Haiti in 2010, health, sanitation and environmental conditions worsened and a large number of persons lost their homes. OBJECTIVE: evaluate vector surveillance with an emphasis on Aedes Aegypti in a post-earthquake displaced persons camp in the commune of Carrefour, Haiti, from June 2010 to June 2011. METHODS: determination was made of the presence and density of Ae. aegypti, Anopheles albimanus and Culex quinquefasciatus by means of larval surveys. Adult control measures and larvicides against Ae. Aegypti were also used. RESULTS: seven mosquito species were identified.The values obtained for the indices household, container and Breteau showed that Ae. aegypti densities remained stable, with an increase in the second half of the year. Most breeding sites were miscellaneous containers (56 %), followed by pneumatic tires (27 %). An. albimanus and Cx. quinquefasciatus showed scant presence in the study area. Adulticide treatments contributed to the control of mosquito densities at institutions with the presence of Cuban cooperation personnel and in the displaced persons camp. CONCLUSIONS: mosquito surveillance and control were permanent during the study period, as a result of which there were no reports of diseases transmitted by this vector in the displaced persons camp.
Descritores: Controle Biológico de Vetores/métodos
Aedes
Larva/crescimento & desenvolvimento
-Haiti
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: lil-737005
Autor: Calderón-Arguedas, Ólger; Troyo, Adriana.
Título: Perfil de resistencia a insecticidas en una cepa de Aedes aegypti (Linnaeus) de la región Caribe de Costa Rica / Insecticidal resistance profiles of an Aedes aegypti (Linnaeus) strain from the Caribbean Region of Costa Rica
Fonte: Rev. cuba. med. trop;66(3):351-359, sep.-dic. 2014.
Idioma: es.
Resumo: Introducción: el dengue constituye la principal enfermedad de transmisión vectorial en Costa Rica. El control del vector Aedes aegypti consiste en la aplicación de piretrinas y temefós, por lo cual es importante monitorear la aparición de resistencia a estos insecticidas. Materiales y métodos: se efectuaron bioensayos con larvas de Ae. aegypti procedentes del cantón de Guácimo en la Región Caribe de Costa Rica. Grupos de 20 larvas fueron expuestos por 24 horas a concentraciones de insecticidas que provocaran una mortalidad entre el 2 y el 100 por ciento. Las pruebas fueron efectuadas por quintuplicado y se calculó la concentración letal 50 por ciento (CL50). Como control susceptible se empleó la cepa Rockefeller. Un radio de resistencia 50 por ciento (RR50) fue calculado para cada insecticida. En caso de resistencia se repitieron los ensayos exponiendo las larvas a butóxido de piperonilo (PB) y S, S, S, tributilfosforotritioato (DEF) para perfilar el mecanismo enzimático vinculado con dicha resistencia. Resultados: no se observó resistencia a temefós y deltametrina, pero sí se encontró resistencia incipiente a la cipermetrina (CL50 = 0,00845 mg/L, rango: 0,00664-0,01038, RR50 = 6,07). El análisis con sinergistas determinó un radio de singergismo (RS) de 19,2 para el PB y de 0,9 para DEF. Discusión: los resultados demuestran que existe un proceso de desarrollo de resistencia a la cipermetrina en los mosquitos Ae. aegypti en esta localidad, el cual está relacionada con la actividad citocromo P450 monooxigenasa. Esto alerta a las autoridades para sustituir dicho insecticida y así asegurar el adecuado control del vector sin la generación de resistencia(AU)

Introduction: dengue is the main vector-borne disease in Costa Rica. The control of the vector Aedes aegypti covers the application of pyrethrins and temephos. For this reason, it is important to monitor the development of resistance to these insecticides. Material and Methods: bioassays were performed using Ae .aegypti larvae from the county of Guacimo in the Caribbean region of Costa Rica. Twenty-larvae groups were exposed to insecticidadl concentrations for 24 hours, which would generate 2 to 100 percent mortality. The tests were performed five times, and a 50 percent lethal concentration (LC50) was calculated. The Rockefeller strain was used as susceptibility control. A 50 percent resistance ratio (RR50) was calculated for each insecticide. When resistance occurred, tests were repeated by exposing the larvae to piperonyl butoxide (PB) and S, S, S, tributylphosphorotrithioate (DEF) in order to determine the enzymatic mechanism associated with this resistance. Results: no resistance to temephos or deltamethrin was observed, but emerging resistance to cypermethrin was detected (LC50 = 0.00845 mg/L, range from 0.00664 to 0.01038, RR50 = 6.07). The synergistic analysis determined a synergism ratio (SR) of 19.2 for PB and 0.9 for DEF. Conclusions: these results show that there is a process of developing resistance to cypermethrin in Ae. aegypti mosquitoes of this county, which is associated with cytochrome P450 monooxygenase activity. This alerts authorities to the need of replacing this insecticide and ensure the appropriate vector control without generating resistance(AU)
Descritores: Resistência a Inseticidas
Controle Biológico de Vetores/métodos
Aedes/imunologia
-Costa Rica/epidemiologia
Vírus da Dengue/patogenicidade
Limites: Animais
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-1293138
Autor: Moscardi, Maurício Lara; Dutra, Viviane; Fernandes, Pablo Kruger; Roggia, Samuel; Levy, Sheila Michele; Falleiros, Angela Maria Ferreira.
Título: Sequencial passages of the multiple nucleopolyhedrovirus of Anagrapha falcifera (Kirby) (Lepidoptera: Noctuidae) (AfMNPV) in Spodoptera cosmioides (Walker) (Lepidoptera, Noctuidae) / Passagens sequenciais do múltiplo nucleopoliedrovírus de Anagrapha falcifera (Kirby) (Lepidoptera: Noctuidae) (AfMNPV) em Spodoptera cosmioides (Walker) (Lepidoptera, Noctuidae)
Fonte: Semina cienc. biol. saude;42(2):221-234, jun./dez. 2021. Tab, Ilus.
Idioma: en.
Resumo: The aim of this work was to evaluate the effects of Anagrapha falcifera multiple nucleopolyhedrovirus (AfMNPV) passages in the Spodoptera cosmioides caterpillar's biology at different times of infection and histological changes that the virus could cause in the caterpillar midgut, seeking correlate histopathologic effects to the effectiveness of this virus as a potential biological control of this pest. Larvae were infected with seven days of development, by using three different passages of AfMNPV on S. cosmioides (F1, F4 and F7, which is the first, fourth and seventh passages, respectively) and the control treatment. Compared biology assays with the same treatments for analyzing behavior and mortality of caterpillars were performed concomitantly. The midgut morphology was compared between infected and uninfected larvae. The digestive tubes were collected at 24, 72 and 144 hours of infection (20 tubes/treatment/time of infection). After collection, the digestive tubes were fixed in Karnovsky, processed, stained with Hematoxylin-Eosin, and examined under a light microscope. The biology results of F4 and F7treatments, showed a drastic reduction in locomotion and feeding from the fourth day after infection and higher cumulative mortality rate compared to the control and F1. All treatments caused morphological changes in the midgut of S. cosmioides, in the three times of infection, with the greatest changes occurring at the epithelium. The AfMNPV, in the three passages tested in S. cosmioides, caused behavioral and morphological changes in the midgut, indicating that it can be a promising agent for biological control of this pest.

O objetivo deste trabalho foi avaliar os efeitos de passagens do múltiplo nucleopoliedrovírus de Anagrapha falcifera (AfMNPV) na biologia de lagartas de Spodoptera cosmioides, em diferentes momentos de infecção, e as alterações histológicas que o vírus poderia causar no intestino médio da lagarta, buscando correlacionar os efeitos histopatológicos e a eficácia deste vírus, como um potencial controle biológico desta praga. As lagartas foram infectadas com sete dias de desenvolvimento, utilizando três passagens diferentes do AfMNPV em S. cosmioides (F1, F4 e F7, sendo a primeira, quarta e sétima passagens, respectivamente) e o tratamento controle. Foram realizados, concomitantemente, ensaios de biologia, comparados com os mesmos tratamentos, para analisar o comportamento e mortalidade das lagartas. A morfologia do intestino médio foi comparada entre as lagartas infectadas e as não infectadas. Os tubos digestivos foram coletados com 24, 72 e 144 horas de infecção (20 tubos/tratamento/ tempo de infecção). Após a coleta, os tubos foram fixados em Karnovsky, processados, corados com Hematoxilina-Eosina e analisados ao microscópio de luz. Os resultados da biologia dos tratamentos F4 e F7 mostraram uma redução drástica na locomoção e alimentação das lagartas, a partir do quarto dia pós-infecção e maior taxa de mortalidade cumulativa, em relação ao controle e F1. Morfologicamente, todos os tratamentos causaram alterações no intestino médio das lagartas de S. cosmioides, nos três tempos de infecção, sendo que as maiores alterações ocorreram no epitélio. O AfMNPV, nas três passagens testadas em S. cosmioides, provocou alterações comportamentais e morfológicas no intestino médio, indicando que pode ser um promissor agente de controle biológico desta praga
Descritores: Controle Biológico de Vetores
Insetos
Lepidópteros
Limites: Animais
Responsável: BR512.1 - Biblioteca Setorial do Centro de Ciências da Saúde


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Id: biblio-1307758
Autor: Balderrama, Fanor; Havez, Victor.
Título: Guia ilustrada para entrenamiento de promotores.
Fonte: Cochabamba; s. ed.; 1992. 20 p.
Idioma: es.
Projeto: M.P.S.S.P.; . U.M.S.S.; Fac. Medicina; Programa Control de la Enfermedad de Chagas; . USAID. Proyecto de Salud Infantil y Comunitaria.
Descritores: Controle Biológico de Vetores
Tipo de Publ: Guia
Monografia
Monografia
Responsável: BO4.1 - Biblioteca Julio Rodríguez Rivas
BO4.1; BO4.1


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Id: biblio-1307757
Autor: Balderrama, Fanor; Havez, Victor.
Título: Guia ilustrada para entrenamiento de promotores.
Fonte: Cochabamba; s. ed.; 1992. 20 p.
Idioma: es.
Projeto: M.P.S.S.P.; . U.M.S.S.; Fac. Medicina; Programa Control de la Enfermedad de Chagas; . USAID. Proyecto de Salud Infantil y Comunitaria.
Descritores: Controle Biológico de Vetores
Tipo de Publ: Guia
Monografia
Monografia
Responsável: BO4.1 - Biblioteca Julio Rodríguez Rivas
BO4.1; BO4.1


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Id: biblio-1011523
Autor: Araujo, Alyson Silva de; Blum, Luiz Eduardo Bassay; Figueiredo, Cícero Célio de.
Título: Biochar and Trichoderma harzianum for the Control of Macrophomina phaseolina
Fonte: Braz. arch. biol. technol;62:e19180259, 2019. tab, graf.
Idioma: en.
Projeto: SUPPORT RESEARCH FOUNDATION OF THE FEDERAL DISTRICT.
Resumo: Abstract This study is based on the importance of biological control methods and the lack of information on the effect of biochar (BCH) from sewage sludge associated or not with Trichoderma harzianum on the control of Macrophomina phaseolina in the bean crop (Phaseolus vulgaris, cv. BRS Estilo). Biochar from sewage sludge, pyrolyzed at 500 ºC and used in low concentration (0.5%), has a direct effect on the in vitro control of M. phaseolina. However, higher BCH concentrations stimulated the growth of the pathogen. In culture medium with or without BCH, T. harzianum (strain 1306) inhibited the mycelial growth of M. phaseolina. The addition of BCH + T. harzianum reduced the deleterious effects caused by M. phaseolina on bean plants. This study demonstrated that joint application of BCH from sewage sludge + T. harzianum considerably increased the fresh and dry mass of bean plants, inoculated or not with M. phaseolina.
Descritores: Esgotos/microbiologia
Trichoderma
Controle Biológico de Vetores/métodos
Phaseolus/microbiologia
Responsável: BR1.1 - BIREME


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Id: biblio-1093548
Autor: González Rizo, Aileen; Companioni Ibañez, Ariamys; Menéndez Díaz, Zulema; Anaya Martínez, Jorge; García García, Israel; Lorenzo Borjas, Claudia M; Castañet, Camilo Ernesto; Gato Armas, Rene.
Título: Evaluación de la eficacia larvicida de Rapidall NP3 (Bacillus thuringiensis) contra Aedes aegypti (Linnaeus) (Diptera: Culicidae) en condiciones de laboratorio / Evaluation of the larvicidal efficacy of Rapidall NP3 (Bacillus thuringiensis) against Aedes aegypti (Linnaeus) (Diptera: Culicidae) in laboratory conditions
Fonte: Rev. cuba. med. trop;71(1):e355, ene.-abr. 2019. tab.
Idioma: es.
Resumo: Los mosquitos son vectores trasmisores de enfermedades como: dengue, zika y chikunguya. El control biológico es una alternativa viable a tener en cuenta por los programas de control. Bacillus thuringiensis es el microorganismo más usado en salud pública. Su efectividad como larvicida contra culícidos depende, en gran medida, de la eficiencia del proceso productivo. El objetivo es evaluar y comparar la eficacia larvicida contra Aedes aegypti de la formulación en polvo Rapidall NP3 con la del producto Bactivec®, formulación líquida utilizada en Cuba. Se realizaron bioensayos de laboratorio según los procedimientos descritos y sugeridos por la OMS para determinar la eficacia, exponiendo las larvas a las concentraciones recomendadas por los fabricantes de los productos Rapidall NP3 y Bactivec®. A los recipientes utilizados se les realizó recambio de agua medio, total y sin recambio, con una frecuencia semanal. A las 24 h se calculó la mortalidad obtenida y se analizó estadísticamente mediante un ANOVA de una vía; cuando existieron diferencias significativas se aplicó un análisis Post-hoc mediante la prueba de Tukey. El producto evaluado Rapidall NP3, provocó 100 por ciento de mortalidad larvaria durante 11 semanas, independientemente de la proporción de recambio de agua, mientras que el Bactivec® solo provocó 100 por ciento de mortalidad hasta las 6 semanas de iniciado el experimento. El biolarvicida Rapidall NP3 mostró buena eficacia y actividad residual prolongada al compararlo con Bactivec®, por lo que recomendamos su evaluación en el terreno en el control larvario de depósitos que constituyen sitios de cría habituales y que no pueden ser eliminados físicamente(AU)

Mosquitoes are vectors of human diseases such as dengue, zika and chikungunya. Biological control is a viable alternative to be taken into account in control programs. Bacillus thuringiensis is the microorganism most commonly used in public health. Its effectiveness as a larvicide against culicides depends to a great extent to the production process. The objectives of the study were to evaluate and compare the larvicidal efficacy against Aedes aegypti of the Rapidall NP3 powder formulation with Bactivec®, a liquid formulation used in Cuba. Laboratory bioassays were conducted to determine efficacy following WHO protocols. Larvae were exposed to the concentrations recommended by Rapidall NP3 and Bactivec® manufacturers. The water in the containers used in the study was replaced once a week: half, whole and no replacement. At 24 hours mortality was estimated and statistically analysis were done by one-way ANOVA. When significant differences were found, post-hoc analysis was performed with Tukey's test. Rapidall NP3 caused 100 percent larval mortality for 11 weeks, regardless of the water replacement proportion, whereas Bactivec® caused 100 percent mortality for only 6 weeks after the start of the experiment. When compared with Bactivec®, biolarvicide Rapidall NP3 displayed better efficacy and more prolonged residual activity. We therefore recommend its field evaluation for larval control of containers constituting habitual breeding sites which cannot be physically eliminated(AU)
Descritores: Controle Biológico de Vetores/métodos
Aedes
Larvicidas/métodos
-Eficácia/métodos
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-1283636
Autor: Peredo, Santiago; Barrera, Claudia; Martínez, José L; Romo, Javier.
Título: Plantas medicinales y aromáticas como hospederas de enemigos naturales de Saissetia oleaeen arreglos espacio-temporales para el cultivo agroecológico de Olea europea / Medicinal and aromatic plants as hosts of natural enemies of Saissetia oleaein spatio-temporal arrangements for the agroecological cultivation of Olea europea
Fonte: Bol. latinoam. Caribe plantas med. aromát;19(5):482-491, 2020. ilus, tab.
Idioma: es.
Resumo: Medicinal and aromatic plants can be considered as "multifunctional plants" due to the diverse properties and ecosystem services they provide in agroecosystems. Among the latter, they regulate the populations of insect pests in crops, harboring their natural enemies. In order to determine the plant species with the greatest presence of natural enemies of Saissetia oleae in an agro-ecological crop of Olea europea, biological corridors with species of medicinal and aromatic plants were established in three sectors of the farm. The plants were collected together with the farmers of the Maipo Province and sticky pheromone traps, vacuum cleaner and entomological umbrella were used to collect the arthropods. The results indicate that Calendula officinalis, Borago officinalis, Aquilea millefolium, Linun usitatissimun, Chamaemulum nobile, Origanum vulgare, Artemisia agrotanum and Symphytum officinale are the species with the highest presence of S. oleae natural enemies identified as Coccophagussp., Metaphycus helvolus, Metaphycus lounsbury, Metaphycussp., Rhyzobius lophanthae, Scutellista caerulea.

Las plantas medicinales y aromáticas pueden ser consideradas como "plantas multifuncionales" por las diversas propiedades y servicios ecosistémicos que prestan en los agroecosistemas. entre estos últimos, regulan las poblaciones de insectos plagas en los cultivos albergando enemigos naturales de éstas con el objetivo de determinar las especies vegetales con mayor presencia de enemigos naturales de Saissetia oleae en un cultivo agroecológico de Olea europea se establecieron corredores biológicos con especies de plantas medicinales y aromáticas en tres sectores de la finca. Las plantas se colectaron junto con los agricultores de la Provincia del Maipo y para la recolección de los artrópodos se utilizaron trampas pegajosas de feromonas, aspirador y paraguas entomológico los resultados indican que Calendula officinalis, Borago officinalis, Aquilea millefolium, Linun usitatissimun, Chamaemulum nobile, Origanum vulgare, Artemisia agrotanum y Symphytum officinale son las especies con mayor presencia de enemigos naturales de S. oleae identificados como Coccophagus sp., Metaphycus helvolus, Metaphycus lounsbury, Metaphycus sp., Rhyzobius lophanthae, Scutellista caerulea.
Descritores: Plantas Medicinais
Controle Biológico de Vetores
Olea/parasitologia
Agricultura Sustentável
Hemípteros
-Chile
Etnobotânica
Biodiversidade
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1283650
Autor: Passos, Bruno Goulart; Rangel, Leonardo da Silva; Albuquerque, Ricardo Diego Duarte Galhardo de; Caldas, Gabriel Rocha; Santos, Marcelo Guerra; Esteves, Ricardo dos Santos; Milton, Flora Aparecida; Neves, Francisco de Assis Rocha; Ruppelt, Bettina Monika; Santos, José Augusto Albuquerque dos; Faria, Robson Xavier; Rocha, Leandro.
Título: Ocotea pulchellaas an alternative against schistosomiasis: chemical analysis, development of nanoemulsion and biological control activity / Ocotea pulchellacomo una alternativa ante esquistosomiasis: análisis químico, desarrollo de una nanoemulsión y actividad en control biológico
Fonte: Bol. latinoam. Caribe plantas med. aromát;19(5):508-518, 2020. tab, ilus.
Idioma: en.
Resumo: The aim of this work was to evaluate the potential of the essential oil (EO) from Ocotea pulchella leaves as an alternative in the control of schistosomiasis. It was tested O. pulchella EO nanoformulation to assess its activity against adult Biomphalaria glabrata, their spawning and Schistossoma mansoni cercariae. Additionally, the EO chemical composition was investigated by gas-chromatography. Nanoemulsion were elaborated by the low energy method. The adult mollusks, their spawning and cercariae were placed in contact with nanoemulsion to calculate lethal concentrations. Myristicin, bicyclogermacrene and α-Pinene were the main substances in the EO. Nanoemulsion caused mortality of adult B. glabrata, its egg embryos and S. mansoni. These results suggest the use of this nanoemulsion as an alternative in the control of the schistosomiasis cycle.

El objetivo de este trabajo fue evaluar el potencial de los aceites esenciales (AE) de las hojas de Ocotea pulchellacomo una alternativa en el control de esquistosomiasis. Se probó una nanoformulación de AE de O. pulchellapara evaluar su actividad ante adultos de Biomphalaria glabrata, sus huevos y cercarías de Schistossoma mansoni. La nanoemulsión fue elaborada por el método de baja energía. Los moluscos adultos, sus huevos y cercarías se colocaron en contacto con la nanoemulsión para calcular concentraciones letales. Los compuestos mayoritarios en el AE fueron miristicina, biciclogermacreno y α-pineno. La nanoemulsión causó mortalidad en adultos de B. glabrata, sus huevos y a S. mansoni. Los resultados sugieren el uso de esta nanoemulsión como una alternativa en el control del ciclo de esquistosomiasis.
Descritores: Esquistossomose/prevenção & controle
Óleos Voláteis/administração & dosagem
Ocotea/química
Emulsões/administração & dosagem
Moluscos/efeitos dos fármacos
-Schistosoma mansoni/efeitos dos fármacos
Biomphalaria/efeitos dos fármacos
Óleos Voláteis/farmacologia
Óleos Voláteis/química
Controle Biológico de Vetores
Cromatografia Gasosa
Sesquiterpenos de Germacrano/análise
Dioxolanos/análise
Emulsões/farmacologia
Cercárias/efeitos dos fármacos
Interações Hidrofóbicas e Hidrofílicas
Derivados de Alilbenzenos/análise
Monoterpenos Bicíclicos/análise
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1055397
Autor: Cai, Jin; Gao, Yichen; Wang, Mengliang; Zhang, Jing; Zhang, Yongbin; Wang, Qi; Liu, Jiquan.
Título: Antibacterial Activity of Polygonum Orientale Extracts Against Clavibacter Michiganensis subsp. michiganensis, the Agent of Bacterial Canker of Tomato Disease
Fonte: Braz. arch. biol. technol;62:e19180021, 2019. tab, graf.
Idioma: en.
Projeto: NATIONAL NATURAL SCIENCE FOUNDATION OF CHINA; . NATURAL SCIENCE FOUNDATION OF SHANXI PROVINCE; . SCIENTIFIC AND TECHNOLOGICAL INNOVATION PROGRAMS OF HIGHER EDUCATION INSTITUTIONS IN SHANXI (STIP).
Resumo: Abstract Clavibacter michiganensis subsp. michiganensis (Cmm), which is a Gram positive bacterium, causes the bacterial canker of tomato disease. The purpose of the study was to evaluate the antibacterial activity of Polygonum orientale extracts against Cmm. In this study, firstly, effects of three extracting parameters (extractive time, extractive temperature, and solid to liquid ratio) of orthogonal experiment design L27 (313) were conducted. Secondly, survival rate was determined and inhibition zone of Cmm rescued post-stress was monitored. Finally, extracellular OD260nm value, extracellular protein content, conformational structure of membrane protein, extracellular alkaline phosphatase (AKP) activity, and ATPase activity were measured to investigate the antibacterial mechanism. Results of orthogonal experiment revealed that extractive time and extractive temperature had highly significant (P<0.01) effects on the antibacterial activity of P. orientale extracts. The optimum conditions were as follows: 10h of extractive time, 60°C of extractive temperature, and 1:20 (g:mL) of solid to liquid ratio. This study also demonstrated that the living cells of each sample from survival rate test had almost no resistance or adaptability, and rescued Cmm cells were much easier to be inhibited by P. orientale extracts. The results of antibacterial mechanism indicated that cell membrane and cell wall of Cmm were seriously damaged by P. orientale extracts, and P. orientale extracts reduced the intracellular ATPase activity dramatically. All these findings suggested that P. orientale extracts had a strong antibacterial activity to inhibit Cmm, and could be used for the ecological management of the bacterial canker of tomato disease.
Descritores: Doenças das Plantas
Controle Biológico de Vetores
Compostos Fitoquímicos
Antibacterianos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME



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