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Id: biblio-838971
Autor: Thunders, Michelle; Cavanagh, Jo; Li, Yinsheng.
Título: De novo transcriptome assembly, functional annotation and differential gene expression analysis of juvenile and adult E. fetida, a model oligochaete used in ecotoxicological studies
Fonte: Biol. Res;50:7, 2017. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Earthworms are sensitive to toxic chemicals present in the soil and so are useful indicator organisms for soil health. Eisenia fetida are commonly used in ecotoxicological studies; therefore the assembly of a baseline transcriptome is important for subsequent analyses exploring the impact of toxin exposure on genome wide gene expression. RESULTS: This paper reports on the de novo transcriptome assembly of E. fetida using Trinity, a freely available software tool. Trinotate was used to carry out functional annotation of the Trinity generated transcriptome file and the transdecoder generated peptide sequence file along with BLASTX, BLASTP and HMMER searches and were loaded into a Sqlite3 database. To identify differentially expressed transcripts; each of the original sequence files were aligned to the de novo assembled transcriptome using Bowtie and then RSEM was used to estimate expression values based on the alignment. EdgeR was used to calculate differential expression between the two conditions, with an FDR corrected P value cut off of 0.001, this returned six significantly differentially expressed genes. Initial BLASTX hits of these putative genes included hits with annelid ferritin and lysozyme proteins, as well as fungal NADH cytochrome b5 reductase and senescence associated proteins. At a cut off of P = 0.01 there were a further 26 differentially expressed genes. CONCLUSION: These data have been made publicly available, and to our knowledge represent the most comprehensive available transcriptome for E. fetida assembled from RNA sequencing data. This provides important groundwork for subsequent ecotoxicogenomic studies exploring the impact of the environment on global gene expression in E. fetida and other earthworm species.
Descritores: Oligoquetos/genética
Perfilação da Expressão Gênica/métodos
Ecotoxicologia
Transcriptoma
-Oligoquetos/efeitos dos fármacos
Poluentes do Solo/toxicidade
Software
Análise de Sequência de RNA/métodos
Toxicogenética/métodos
Exposição Ambiental
Ontologia Genética
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  2 / 96 LILACS  
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Id: biblio-1025893
Autor: Guatemala. (Leyes, etc.).
Título: Creación del Ministerio del Medio Ambiente y Recursos Naturales: decreto 90-2000 / Creation of the Ministry of Environment and Natural Resources: decree 90-2000.
Fonte: Guatemala; Congreso de la República de Guatemala; 11 dic. 2000. 5 p.
Idioma: es.
Resumo: Modifica DCX 114-97 Ley del Organismo Ejecutivo, artículos: - 19, se adiciona el numeral 13, el cual establece "Ministerio de Ambiente y Recursos Naturales; - 29, Ministerio de Agricultura Ganadería y Alimentación - 29, se adiciona artículo 29 bis, el cual establece, Ministerio de Ambiente y Recursos Naturales - 34, literal e), regula el cumplimiento de las normas y especificaciones ambientales que en materia de recursos no renovables establezca el Ministerio de Ambiente y Recursos Naturales; Modifica DCX 68-86 Ley de Protección y Mejoramiento del Medio Ambiente, artículo 2, establece que la aplicación de esta ley y sus reglamentos compete el Organismo Ejecutivo a través del Ministerio de Ambiente y Recursos Naturales cuyas funciones establece la Ley del Organismo Ejecutivo; Deroga art. 20, 21, 22, 23, 24, 25, 26, 27, 28 y 38. Modifica DCX 101-96 Ley Forestal, artículo 10, literales a) y h); a) El Ministerio de Ambiente y Recursos Naturales quien la preside y nombrará a su suplente; h) Un representante titular y su suplente designado por el Ministro de Agricultura, Ganadería y Alimentación; Deroga AGX 35-2000. 12 Artículos
Descritores: Desequilíbrio Ecológico/legislação & jurisprudência
Equilíbrio Ecológico/legislação & jurisprudência
Meio Ambiente
Poluentes Ambientais
Poluição Ambiental/legislação & jurisprudência
-Poluentes do Solo
Poluentes da Água/administração & dosagem
Monitoramento do Solo
Recursos Naturais
Reservas Naturais/legislação & jurisprudência
Gestão dos Recursos Naturais/legislação & jurisprudência
Guatemala
Limites: Humanos
Masculino
Feminino
Responsável: GT1.1 - Biblioteca y Centro de Información


  3 / 96 LILACS  
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Id: biblio-886775
Autor: ZOCCHE, JAIRO J; ROHR, PAULA; DAMIANI, ADRIANI P; LEFFA, DANIELA D; MARTINS, MIRIAM C; ZOCCHE, CAROLINE M; TEIXEIRA, KARINA O; BORGES, GABRIELA D; JESUS, MAIELLEN M DE; SANTOS, CARLA E I DOS; DIAS, JOHNNY F; ANDRADE, VANESSA M DE.
Título: Elemental composition of vegetables cultivated over coal-mining waste
Fonte: An. acad. bras. ciênc;89(3,supl):2383-2398, 2017. tab.
Idioma: en.
Resumo: ABSTRACT We assessed elemental composition of the liver in mice subjected to one-time or chronic consumption of the juice of vegetables cultivated in a vegetable garden built over deposits of coal waste. Lactuca sativa L. (lettuce), Beta vulgaris L. (beet), Brassica oleracea L. var. italica (broccoli) and Brassica oleracea L. var. acephala (kale) were collected from the coal-mining area and from a certified organic farm (control). Elemental composition was analyzed by particle-induced X-ray emission (PIXE) method. Concentrations of Mg, S, and Ca of mice subjected to one-time consumption of broccoli and concentrations of these same elements plus Si of mice receiving kale were higher in the coal-mining area. Concentrations of P, K, and Cu were increase after chronic consumption of lettuce from the coal-mining area, whereas the levels of Si, P, K, Fe, and Zn were higher in the group consuming kale from the coal-mining area. Our data suggests that people consuming vegetables grown over coal wastes may ingest significant amounts of chemical elements that pose a risk to health, since these plants contain both essential and toxic metals in a wide range of concentrations, which can do more harm than good.
Descritores: Esgotos/química
Poluentes do Solo/análise
Verduras/química
Contaminação de Alimentos/análise
Minas de Carvão
Fígado/efeitos dos fármacos
-Poluentes do Solo/toxicidade
Verduras/toxicidade
Eliminação de Resíduos Líquidos
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


  4 / 96 LILACS  
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Id: biblio-962073
Autor: Fontes, André M; Gusson, Vinícius P; Souza, Anelise A. de; Souza, Marco A. de.
Título: Identification of enteroparasites in recreation areas of elementary schools in Northern Espírito Santo, Brazil / Identificación de enteroparásitos en áreas de recreación de escuelas primarias en el norte de Espirito Santo, Brasil
Fonte: Rev. salud pública;19(6):795-799, nov.-dic. 2017. tab.
Idioma: en.
Resumo: ABSTRACT Objective Since sand is an important child means of contagion by enteroparasites, this study aimed to conduct a parasitological evaluation in recreation areas of elementary schools and early childhood education centers, in São Mateus-ES. Material and Methods Sand samples were collected in seven municipal elementary schools and in a municipal kindergarten and nursery center. For the recovery of helminth eggs and protozoan cysts, flotation and spontaneous sedimentation techniques were used. Results It was found that 77 % of the analyzed institutions were positive for helminth eggs and larvae. Among the eggs and larvae identified, the main were Ascaris lumbricoides and Toxocara canis. Pearson's chi-square test identified the best results when using the spontaneous sedimentation method. Conclusions Considering that individuals, especially children living in poor sanitation conditions, are more prone to enteroparasites, studies point to the need of the application of preventive and health education measures for the population enrolled in municipal schools in São Mateus.(AU)

RESUMEN Objetivo Dado que la arena es un medio importante de contagio infantil por enteroparásitos, este estudio tuvo como objetivo realizar una evaluación parasitológica en áreas recreativas de escuelas primarias y centros de educación infantil, en São Mateus-ES. Materiales y Métodos Se tomaron muestras de arena en siete escuelas primarias municipales, en un jardín de infantes y en una guardería municipal. Para la recuperación de huevos de helmintos y quistes de protozoos, se usaron las técnicas de flotación y de sedimentación espontánea. Resultados Se encontró que el 77 % de las instituciones analizadas dieron positivo para huevos y larvas de helmintos. Entre los huevos y larvas identificados los principales fueron Ascaris lumbricoides y Toxocara canis. La prueba de ji-cuadrado de Pearson identifica los mejores resultados cuando se usa el método de sedimentación espontánea. Conclusión Teniendo en cuenta que las personas, especialmente los niños que viven en malas condiciones de saneamiento, son más propensas a enteropatías, los estudios apuntan a la necesidad de la aplicación de medidas preventivas y de educación sanitaria para la población inscrita en las escuelas municipales de São Mateus.(AU)
Descritores: Parasitos
Poluentes do Solo/análise
Saúde da Criança
Helmintos/isolamento & purificação
-Brasil
Sedimentação/métodos
Limites: Humanos
Criança
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  5 / 96 LILACS  
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Id: biblio-1087172
Autor: Salam, Menaka; Varma, Ajit.
Título: Bacterial community structure in soils contaminated with electronic waste pollutants from Delhi NCR, India
Fonte: Electron. j. biotechnol;41:72-80, sept. 2019. ilus, tab, graf.
Idioma: en.
Projeto: DST-SERB, Government of India, Young Scientist Scheme.
Resumo: Background: Microbial community analysis of electronic waste (e-waste)-polluted environments is of interest to understand the effect of toxic e-waste pollutants on the soil microbial community and to evaluate novel microorganisms resisting the toxic environment. The present study aims to investigate the bacterial community structure in soils contaminated with e-waste from various sites of Loni and Mandoli (National Capital Region (NCR), India) where e-waste dumping and recycling activities are being carried out for many years. Results: Interferences to soil metagenomic DNA extraction and PCR amplification were observed because of the presence of inhibiting components derived from circuit boards. Whole-metagenome sequencing on the Illumina MiSeq platform showed that the most abundant phyla were Proteobacteria and Firmicutes. Deltaproteobacteria and Betaproteobacteria were the most common classes under Proteobacteria. Denaturing gradient gel electrophoresis (DGGE) analysis of the bacterial 16S rRNA gene showed that e-waste contamination altered the soil bacterial composition and diversity. There was a decrease in the number of predominant bacterial groups like Proteobacteria and Firmicutes but emergence of Actinobacteria in the contaminated soil samples. Conclusions: This is the first report describing the bacterial community structure of composite soil samples of ewaste-contaminated sites of Loni and Mandoli, Delhi NCR, India. The findings indicate that novel bacteria with potential bioremediating properties may be present in the e-waste-contaminated sites and hence need to be evaluated further.
Descritores: Microbiologia do Solo
Bactérias/isolamento & purificação
Bactérias/genética
Resíduo Eletrônico/análise
-Poluentes do Solo
Reação em Cadeia da Polimerase
Metais Pesados
Proteobactérias/isolamento & purificação
Metagenômica
Eletroforese em Gel de Gradiente Desnaturante
Microbiota
Firmicutes/isolamento & purificação
Índia
Responsável: CL1.1 - Biblioteca Central


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Id: lil-432168
Autor: Capuano, Divani Maria; Rocha, Gutemberg de Melo.
Título: Contaminação de areias em áreas de recreação infantil por ovos e larvas de Ancylostoma sp no município de Ribeirão Preto, SP, Brasil / Contamination of sandpits by Ancylostoma sp. eggs and larvae in children recreational areas of Ribeirão Preto county, SP, Brazil
Fonte: Rev. Inst. Adolfo Lutz;64(1):142-144, jan.-jun. 2005. tab.
Idioma: pt.
Resumo: As areias de áreas de lazer podem representar um importante ecótopo para a transmissão de várias zoonosesparasitárias, dentre elas a Larva Migrans Cutânea (LMC), causada pela penetração na pele humana das larvas de ancilostomídeos de cães e gatos. Neste estudo investigou-se a contaminação dos tanques de areia por ovos e/ou larvas de Ancylostoma sp. das áreas de recreação infantil existentes em 11 praças públicas do município de Ribeirão Preto. Entre maio a dezembro de 2003, foram coletadas cinco amostras de areia de cada área de recreação. A recuperação das larvas foi realizada pelo método de Baermann, enquanto que para a detecção dos ovos utilizou-se a técnica da centrífugo-flutuação com soluçõessaturadas de sulfato de magnésio contendo 5% de iodeto de potássio (d = 1,33 g/cm3) e de sulfato de zinco(d = 1,20 g/cm3), após um lavado prévio com Tween 80. A presença de larvas foi observada em seis (54%) áreas de recreação, sendo que em quatro (36%) delas foram encontrados ovos de Ancylostoma sp. Estes resultados indicam um risco para a ocorrência da LMC nos usuários destas áreas
Descritores: Ancylostoma
Areia
Larva Migrans
Poluentes do Solo
Zonas de Recreação
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-974306
Autor: Dealtry, Simone; Ghizelini, Angela Michelato; Mendonça-Hagler, Leda C. S; Chaloub, Ricardo Moreira; Reinert, Fernanda; Campos, Tácio M. P. de; Gomes, Newton C. M; Smalla, Kornelia.
Título: Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana
Fonte: Braz. j. microbiol;49(4):757-769, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Deutsche Forschungsgemeinschaft; . FAPERJ-Brazil.
Resumo: ABSTRACT Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition. However, while the bacterial community was rather stable, pronounced shifts were observed in the abundance of bacteria carrying plasmids. A PCR-Southern blot hybridization analysis indicated an increase in the abundance of IncP-9 catabolic plasmids. Denaturing gradient gel electrophoresis of naphthalene dioxygenase (ndo) genes amplified from cDNA (RNA) indicated the dominance of a specific ndo gene in the inoculated petroleum amendment treatment. The petroleum hydrocarbon degrading consortium characterization indicated the prevalence of bacteria assigned to Pseudomonas spp., Comamonas spp. and Ochrobactrum spp. IncP-9 plasmids were detected for the first time in Comamonas sp. and Ochrobactrum spp., which is a novelty of this study.
Descritores: Bactérias/isolamento & purificação
Bactérias/metabolismo
Avicennia/microbiologia
Hidrocarbonetos/metabolismo
-Plasmídeos/genética
Plasmídeos/metabolismo
Poluentes do Solo/análise
Poluentes do Solo/metabolismo
Bactérias/classificação
Bactérias/genética
Biodegradação Ambiental
DNA Bacteriano/genética
Petróleo/análise
RNA Ribossômico 16S/genética
Poluição por Petróleo/análise
Avicennia/metabolismo
Rizosfera
Responsável: BR1.1 - BIREME


  8 / 96 LILACS  
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Id: biblio-839370
Autor: Leal, Aline Jaime; Rodrigues, Edmo Montes; Leal, Patrícia Lopes; Júlio, Aline Daniela Lopes; Fernandes, Rita de Cássia Rocha; Borges, Arnaldo Chaer; Tótola, Marcos Rogério.
Título: Changes in the microbial community during bioremediation of gasoline-contaminated soil
Fonte: Braz. j. microbiol;48(2):342-351, April.-June 2017. tab, graf.
Idioma: en.
Resumo: Abstract We aimed to verify the changes in the microbial community during bioremediation of gasoline-contaminated soil. Microbial inoculants were produced from successive additions of gasoline to municipal solid waste compost (MSWC) previously fertilized with nitrogen-phosphorous. To obtain Inoculant A, fertilized MSWC was amended with gasoline every 3 days during 18 days. Inoculant B received the same application, but at every 6 days. Inoculant C included MSWC fertilized with N–P, but no gasoline. The inoculants were applied to gasoline-contaminated soil at 10, 30, or 50 g/kg. Mineralization of gasoline hydrocarbons in soil was evaluated by respirometric analysis. The viability of the inoculants was evaluated after 103 days of storage under refrigeration or room temperature. The relative proportions of microbial groups in the inoculants and soil were evaluated by FAME. The dose of 50 g/kg of inoculants A and B led to the largest CO2 emission from soil. CO2 emissions in treatments with inoculant C were inversely proportional to the dose of inoculant. Heterotrophic bacterial counts were greater in soil treated with inoculants A and B. The application of inoculants decreased the proportion of actinobacteria and increased of Gram-negative bacteria. Decline in the density of heterotrophic bacteria in inoculants occurred after storage. This reduction was bigger in inoculants stored at room temperature. The application of stored inoculants in gasoline-contaminated soil resulted in a CO2 emission twice bigger than that observed in uninoculated soil. We concluded that MSWC is an effective material for the production of microbial inoculants for the bioremediation of gasoline-contaminated soil.
Descritores: Microbiologia do Solo
Poluentes do Solo/metabolismo
Gasolina
Carcinógenos Ambientais/metabolismo
Biota/efeitos dos fármacos
-Biodegradação Ambiental
Dióxido de Carbono/análise
Carga Bacteriana
Hidrocarbonetos/análise
Responsável: BR1.1 - BIREME


  9 / 96 LILACS  
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Id: biblio-828206
Autor: Lopes Leal, Patrícia; Varón-López, Maryeimy; Gonçalves de Oliveira Prado, Isabelle; Valentim dos Santos, Jessé; Fonsêca Sousa Soares, Cláudio Roberto; Siqueira, José Oswaldo; de Souza Moreira, Fatima Maria.
Título: Enrichment of arbuscular mycorrhizal fungi in a contaminated soil after rehabilitation
Fonte: Braz. j. microbiol;47(4):853-862, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Spore counts, species composition and richness of arbuscular mycorrhizal fungi, and soil glomalin contents were evaluated in a soil contaminated with Zn, Cu, Cd and Pb after rehabilitation by partial replacement of the contaminated soil with non-contaminated soil, and by Eucalyptus camaldulensis planting with and without Brachiaria decumbens sowing. These rehabilitation procedures were compared with soils from contaminated non-rehabilitated area and non-contaminated adjacent soils. Arbuscular mycorrhizal fungi communities attributes were assessed by direct field sampling, trap culture technique, and by glomalin contents estimate. Arbuscular mycorrhizal fungi was markedly favored by rehabilitation, and a total of 15 arbuscular mycorrhizal fungi morphotypes were detected in the studied area. Species from the Glomus and Acaulospora genera were the most common mycorrhizal fungi. Number of spores was increased by as much as 300-fold, and species richness almost doubled in areas rehabilitated by planting Eucalyptus in rows and sowing B. decumbens in inter-rows. Contents of heavy metals in the soil were negatively correlated with both species richness and glomalin contents. Introduction of B. decumbens together with Eucalyptus causes enrichment of arbuscular mycorrhizal fungi species and a more balanced community of arbuscular mycorrhizal fungi spores in contaminated soil.
Descritores: Solo/química
Microbiologia do Solo
Brasil
Micorrizas/classificação
Poluição Ambiental
-Poluentes do Solo/química
Esporos Fúngicos
Proteínas Fúngicas
Contagem de Colônia Microbiana
Metais Pesados/química
Responsável: BR1.1 - BIREME


  10 / 96 LILACS  
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Id: lil-780845
Autor: Zhou, Lisha; Li, Hui; Zhang, Ying; Han, Siqin; Xu, Hui.
Título: Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities
Fonte: Braz. j. microbiol;47(2):271-278, Apr.-June 2016. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Strategic Priority Research Program of the Chinese Academy of Sciences.
Resumo: Abstract Members of the Sphingomonas genus are often isolated from petroleum-contaminated soils due to their unique abilities to degrade polycyclic aromatic hydrocarbons (PAHs), which are important for in situ bioremediation. In this study, a combined phenotypic and genotypic approach using streptomycin-containing medium and Sphingomonas -specific PCR was developed to isolate and identify culturable Sphingomonas strains present in petroleum-contaminated soils in the Shenfu wastewater irrigation zone. Of the 15 soil samples examined, 12 soils yielded yellow streptomycin-resistant colonies. The largest number of yellow colony-forming units (CFUs) could reach 105 CFUs g-1 soil. The number of yellow CFUs had a significant positive correlation (p < 0.05) with the ratio of PAHs to total petroleum hydrocarbons (TPH), indicating that Sphingomonas may play a key role in degrading the PAH fraction of the petroleum contaminants at this site. Sixty yellow colonies were selected randomly and analyzed by colony PCR using Sphingomonas -specific primers, out of which 48 isolates had PCR-positive signals. The 48 positive amplicons generated 8 distinct restriction fragment length polymorphism (RFLP) patterns, and 7 out of 8 phylotypes were identified as Sphingomonas by 16S rRNA gene sequencing of the representative strains. Within these 7 Sphingomonas strains, 6 strains were capable of using fluorene as the sole carbon source, while 2 strains were phenanthrene-degrading Sphingomonas. To the best of our knowledge, this is the first report to evaluate the relationship between PAHs contamination levels and culturable Sphingomonas in environmental samples.
Descritores: Hidrocarbonetos Policíclicos Aromáticos/metabolismo
Microbiologia do Solo
Poluentes do Solo/metabolismo
Sphingomonas/isolamento & purificação
Sphingomonas/metabolismo
-Filogenia
Biodegradação Ambiental
Petróleo/análise
China
Sphingomonas/classificação
Sphingomonas/genética
Responsável: BR1.1 - BIREME



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