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Id: biblio-1055399
Autor: LEBLEBİCİ, Zeliha; DALMIŞ, Esra; ANDEDEN, Enver Ersoy.
Título: Determination of the potential of Pistia stratiotes L. in removing nickel from the environment by utilizing its rhizofiltration capacity
Fonte: Braz. arch. biol. technol;62:e19180487, 2019. tab, graf.
Idioma: en.
Projeto: Nevsehir Hacı Bektaş Veli University Research Fund.
Resumo: Abstract: The aim of this study is to explain the biological response and rhizofiltration capacity of Pistia stratiotes, which is an aquatic macrophyte, in alleviating heavy metal stress. In our study, Pistia species were exposed to Nickel (Ni) at different concentrations over seven days. The potential of the aquatic macrophytes in accumulating heavy metals in the water and in removing them from the environment was evaluated by determining the bio-concentration factors (BCF). Selected biological parameters in the leaves of Pistia stratiotes, including metal accumulation, photosynthetic pigment amount, lipid peroxidation activity, and growth rates were measured at the end of the seven-day period. The highest amount of Ni accumulation in P. stratiotes occurred at a concentration of 20 mg l-1. The amount of chlorophyll a (chl a) in P. stratiotes reached a value of 0.087 mg g-1 at the Ni concentration of 20 mg l-1. The relative growth rate (RGR) of P. stratiotes showed a negative correlation with the increases in the concentration levels of the metal. Malondialdehyde (MDA) levels increased to 8.214 nmol/g at the concentration of 20 mg l-1, depending on Ni concentration and time. In our study, the use of P. stratiotes has been determined to be an appropriate plant as an effective Ni accumulator to be utilized with the purpose of rhizofiltration.
Descritores: Biodegradação Ambiental
Metais Pesados
Araceae
Bioacumulação
Responsável: BR1.1 - BIREME


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Id: biblio-1055419
Autor: Bernardo, Bernardete da Silva; Ramos, Rodrigo Ferraz; Callegaro, Kelly; Daroit, Daniel Joner.
Título: Co-production of Proteases and Bioactive Protein Hydrolysates from Bioprocessing of Feather Meal
Fonte: Braz. arch. biol. technol;62:e19180621, 2019. graf.
Idioma: en.
Projeto: CNPq.
Resumo: Abstract: Feather meal conversion through submerged cultivations with Bacillus strains (CL33A, CL14) yielded proteases and protein hydrolysates. After 4-day (CL33A) and 10-day (CL14) cultivations, protease activities reached 461 U/mL; hydrolysates presented antioxidant (radicals-scavenging, 57-77%; Fe2+-chelation, 14-28%; Fe3+-reduction) and antidiabetic (dipeptidyl peptidase-IV inhibition, 49-52%) potentials. The obtained bioproducts present prospective commercial/industrial applications.
Descritores: Bacillus
Biotecnologia/métodos
Hipoglicemiantes
Antioxidantes
-Biodegradação Ambiental
Responsável: BR1.1 - BIREME


  3 / 383 LILACS  
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Id: biblio-1055421
Autor: Enzyme Biotechnology LaboratoryLugani, Yogita; Kauldhar, Baljinder Singh; Enzyme Biotechnology LaboratoryKaur, Navneet; Enzyme Biotechnology LaboratorySooch, Balwinder Singh.
Título: Phenotypic Characterization and Synthesis of Extracellular Catecholase from a Newly Isolated Bacterium Pseudomonas sp. BSC-6
Fonte: Braz. arch. biol. technol;62:e19180360, 2019. tab, graf.
Idioma: en.
Resumo: Abstract Catecholase (EC 1.10.3.1), an oxidoreductase enzyme is a key member of polyphenol oxidase family which catalyze the degradation of catechol. This enzyme possesses vast applications in diverse areas and is found in bacteria, fungi, mushrooms, higher plants, arthropods, amphibians and mammals. Catechol, a phenolic compound, is used as a starting material in the synthesis of various industrial compounds such as inhibitors, antioxidants, pesticides etc. The release of this phenolic compound in the environment causes toxicity to both flora and fauna. In the present studies, emphasis has been laid on isolation, screening and characterization of catechol degrading bacterium coupled with synthesis of catecholase enzyme. Further, the selected isolated strain was phenotypically characterized and was found to be member of genus Pseudomonas. Among all the isolates, BSC-6 was found as best isolate with maximum extracellular catecholase activity of 152.32 IU/L obtained after scale up studies. The herein synthesized bacterial catecholase may be employed for wide applications particularly in bioremediation of phenol enriched polluted sites.
Descritores: Oxirredutases
Catecóis
Polifenóis
-Pseudomonas
Biodegradação Ambiental
Responsável: BR1.1 - BIREME


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Id: biblio-1285559
Autor: Albertia, María Melisa; Cunha, Matheus Luis Oliveira; Mendes, Danilo Wanderley; Vieira Junior, Wagner Gonçalves; Zied, Diego Cunha.
Título: Tecnologic Development on Pleurotus Cultivation: Specific Practices Used in Brazil
Fonte: Braz. arch. biol. technol;64:e21200198, 2021. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo a Pesquisa do Estado de São Paulo.
Resumo: HIGHLIGHTS P. ostreatus and P. sapidus are the most productive species under the evaluated conditions. Different growing systems are suitable for the production of P. ostreatus var. Florida. Temperature control level affects differently the P. ostreatus var. Florida isolates. Environmental and strain factors affect yield and production parameters of P. ostreatus var. Florida.

Abstract In Brazil, Pleurotus is the most important mushroom produced especially P. ostreatus var. Florida. In this country as in many others, the great potential for mushroom cultivation remains unexplored. Therefore, it is very important to develop new studies that allow optimizing its production. The aims of the manuscript were: i) to evaluate the productivity of six different species of Pleurotus (P. citrinopileatus; P. djamor; P. ostreatus; P. ostreatus var. Florida; P. pulmonarius; P. sapidus); ii) to measure the effect of three different environmental conditions during cultivation of three isolates of P. ostreatus var. Florida. As results, P. ostreatus and P. sapidus were the most productive isolates under the evaluated conditions. Different environments produced variable effects according to the P. ostreatus var. Florida isolates, being possible to observe a highly plastic strain (POF 02/18), a highly sensitive strain (POF 03/18) and a strain with variable responses (POF 01/18).
Descritores: Biodegradação Ambiental
Produção de Alimentos
Pleurotus/isolamento & purificação
-Brasil
Agroindústria/métodos
Responsável: BR1.1 - BIREME


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Id: biblio-1047375
Autor: Li, Shanshan; Qin, Kun; Li, Huaying; Guo, Jin; Li, Dejin; Liu, Fang; Tan, Zhilei; Yan, Wei; Qu, Shuling; Zhao, Huabing.
Título: Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6
Fonte: Electron. j. biotechnol;34:83-90, july. 2018. tab, ilus, graf.
Idioma: en.
Projeto: Tianjin Research Program of Application Foundation and Advanced Technology; . National Natural Science Foundation of China.
Resumo: Background: Although the functional redundancy of catechol 1,2-dioxygenase (C12O) genes has been reported in several microorganisms, limited enzymes were characterised, let alone the advantage of the coexistence of the multiple copies of C12O genes. Results: In this study, four novel C12O genes, designated catA, catAI, catAII and catAIII, in the naphthalene-degrading strain Pseudomonas putida ND6, were cloned and characterised. Phylogenetic analysis of their deduced amino acid sequences revealed that the four C12O isozymes each formed independent subtrees, together with homologues from other organisms. All four enzymes exhibited maximum activity at pH 7.4 and higher activity in alkaline than in acidic conditions. Furthermore, CatA, CatAI and CatAIII were maximally active at a temperature of 45°C, whereas a higher optimum temperature was observed for CatAII at a temperature of 50°C. CatAI exhibited superior temperature stability compared with the other three C12O isozymes, and kinetic analysis indicated similar enzyme activities for CatA, CatAI and CatAII, whereas that of CatAIII was lower. Significantly, among metal ions tested, only Cu2+ substantially inhibited the activity of these C12O isozymes, thus indicating that they have potential to facilitate bioremediation in environments polluted with aromatics in the presence of metals. Moreover, gene expression analysis at the mRNA level and determination of enzyme activity clearly indicated that the redundancy of the catA genes has increased the levels of C12O. Conclusion: The results clearly imply that the redundancy of catA genes increases the available amount of C12O in P. putida ND6, which would be beneficial for survival in challenging environments.
Descritores: Pseudomonas putida/enzimologia
Pseudomonas putida/genética
Catecol 1,2-Dioxigenase/genética
-Temperatura
Biodegradação Ambiental
Clonagem Molecular
Catecol 1,2-Dioxigenase/análise
Catecol 1,2-Dioxigenase/metabolismo
Genes Bacterianos
Concentração de Íons de Hidrogênio
Isoenzimas
Metais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1047766
Autor: Franchi, Oscar; Rosenkranz, Francisca; Chamy, Rolando.
Título: Key microbial populations involved in anaerobic degradation of phenol and p-cresol using different inocula
Fonte: Electron. j. biotechnol;35:33-38, sept. 2018. graf.
Idioma: en.
Projeto: CONICYT; . FONDECYT.
Resumo: Background: Anaerobic digestion is an alternative bioprocess used to treat effluents containing toxic compounds such as phenol and p-cresol. Selection of an adequate sludge as inoculum containing an adapted microbial consortium is a relevant factor to improve the removal of these pollutants. The objective of this study is to identify the key microorganisms involved in the anaerobic digestion of phenol and p-cresol and elucidate the relevance of the bamA gene abundance (a marker gene for aromatic degraders) in the process, in order to establish new strategies for inocula selection and improve the system's performance. Results: Successive batch anaerobic digestion of phenol and p-cresol was performed using granular or suspended sludge. Granular sludge in comparison to suspended sludge showed higher degradation rates both for phenol (11.3 ± 0.7 vs 8.1 ± 1.1 mg l-1 d-1) and p-cresol (7.8 ± 0.4 vs 3.7 ± 1.0 mg l-1 d-1). After three and four re-feedings of phenol and p-cresol, respectively, the microbial structure from both sludges was clearly different from the original sludges. Anaerobic digestion of phenol and p-cresol generated an abundance increase in Syntrophorhabdus genus and bamA gene, together with hydrogenotrophic and aceticlastic archaea. Analysis of results indicates that differences in methanogenic pathways and levels of Syntrophorhabdus and bamA gene in the inocula, could be the causes of dissimilar degradation rates between each sludge. Conclusions: Syntrophorhabdus and bamA gene play relevant roles in anaerobic degradation of phenolics. Estimation of these components could serve as a fast screening tool to find the most acclimatized sludge to efficiently degrade mono-aromatic compounds.
Descritores: Bactérias/metabolismo
Digestão Anaeróbia
Fenol/metabolismo
Cresóis/metabolismo
-Fenóis/metabolismo
Esgotos
Biodegradação Ambiental
Deltaproteobacteria
Consórcios Microbianos
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1051261
Autor: Massoud, Ramona; Hadiani, Mohammad Rasoul; Hamzehlou, Pegah; Khosravi-Darani, Kianoush.
Título: Bioremediation of heavy metals in food industry: application of Saccharomyces cerevisiae
Fonte: Electron. j. biotechnol;37:56-60, Jan. 2019. tab.
Idioma: en.
Projeto: Shahid Beheshti University of Medical Sciences.
Resumo: Heavy metals are natural elements in the Earth's crust that can enter human food through industrial or agricultural processing, in the form of fertilizers and pesticides. These elements are not biodegradable. Some heavy metals are known as pollutants and are toxic, and their bioaccumulation in plant and animal tissues can cause undesirable effects for humans; therefore, their amount in water and food should always be under control. The aim of this study is to investigate the conditions for the bioremediation of heavy metals in foods. Various physical, chemical, and biological methods have been used to reduce the heavy metal content in the environment. During the last decades, bioremediation methods using plants and microorganisms have created interest to researchers for their advantages such as being more specific and environmentally friendly. The main pollutant elements in foods and beverages are lead, cadmium, arsenic, and mercury, which have their own permissible limits. Among the microorganisms that are capable of bioremediation of heavy metals, Saccharomyces cerevisiae is an interesting choice for its special characteristics and being safe for humans, which make it quite common and useful in the food industry. Its mass production as the byproduct of the fermentation industry and the low cost of culture media are the other advantages. The ability of this yeast to remove an individual separated element has also been widely investigated. In countries with high heavy metal pollution in wheat, the use of S. cerevisiae is a native solution for overcoming the problem of solution. This article summarizes the main conditions for heavy metal absorption by S. cerevisiae.
Descritores: Saccharomyces cerevisiae/metabolismo
Biodegradação Ambiental
Indústria Alimentícia
Metais Pesados/metabolismo
-Arsênio
Leveduras
Cádmio
Remoção de Contaminantes
Absorção
Bioacumulação
Chumbo
Mercúrio
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1249212
Autor: Parwani, Laxmi; Bhatt, Medha; Singh, Jaspreet.
Título: Potential Biotechnological Applications of Cyanobacterial Exopolysaccharides
Fonte: Braz. arch. biol. technol;64:e21200401, 2021.
Idioma: en.
Resumo: Abstract The cyanobacterial exopolysaccharides (EPSs) are considered as one of the important group of biopolymers having significant ecological, industrial, and biotechnological importance. Cyanobacteria are regarded as a very abundant source of structurally diverse, high molecular weight polysaccharides having variable composition and roles according to the organisms and the environmental conditions in which they are produced. Due to their structural complexity, versatility and valuable biological properties, they are now emerging as high-value compounds. They are possessing exceptional properties and thus are being widely explored for various applications like in food and pharmaceutical industries, in bioremediation for removal of heavy metals, for soil conditioning, as biopolymers, bioadhesives, and bioflocculants. However, poor understanding of their complex structural properties, lack of concrete information regarding the genes encoding the proteins involved in the EPS biosynthetic pathways, their process of production and about the associated factors controlling their structural stability, strongly limits their commercialization and applications in the various fields of biotechnology. Owing to the above context, the present review is aimed to organize the available information on applications of cyanobacterial EPSs in the field of biotechnology and to identify the research gaps for improved industrial utilization and commercialization of these biomaterials.
Descritores: Biodegradação Ambiental
Biotecnologia/métodos
Cianobactérias
-Polissacarídeos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-1254671
Autor: Ibrahim, Salihu; Mohd Zahri, Khadijah Nabilah; Convey, Peter; Abdul Khalil, Khalilah; Gomez-Fuentes, Claudio; Zulkarnain, Azham; Alias, Siti Aisyah; González-Rocha, Gerardo; Ahmad, Siti Aqlima.
Título: Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica
Fonte: Electron. j. biotechnol;48:1-12, nov. 2020. tab, graf, ilus.
Idioma: en.
Projeto: PUTRA; . PUTRA-IPS; . PUTRA Berimpak.
Resumo: BACKGROUND: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms and communities. The removal of oils such as canola oil from the environment and wastewater using biological approaches is desirable as the thermal process of oil degradation is expensive and ineffective. RESULTS: Rhodococcus sp. AQ5-07 was found to have high canola oil-degrading ability. Physico-cultural conditions influencing its activity were studied using one-factor-at-a-time (OFAT) and statistical optimisation approaches. Considerable degradation (78.60%) of 3% oil was achieved by this bacterium when incubated with 1.0 g/L ammonium sulphate, 0.3 g/L yeast extract, pH 7.5 and 10% inoculum at 10°C over a 72-h incubation period. Optimisation of the medium conditions using response surface methodology (RSM) resulted in a 9.01% increase in oil degradation (87.61%) when supplemented with 3.5% canola oil, 1.05 g/L ammonium sulphate, 0.28g/L yeast extract, pH 7.5 and 10% inoculum at 12.5°C over the same incubation period. The bacterium was able to tolerate an oil concentration of up to 4.0%, after which decreased bacterial growth and oil degradation were observed. CONCLUSIONS: These features make this strain worthy of examination for practical bioremediation of lipid-rich contaminated sites. This is the first report of any waste catering oil degradation by bacteria originating from Antarctica.
Descritores: Rhodococcus/fisiologia
Óleo de Brassica napus/metabolismo
-Resíduos
Biodegradação Ambiental
Adaptação Fisiológica
Temperatura Baixa
Águas Residuárias
Concentração de Íons de Hidrogênio
Regiões Antárticas
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1223238
Autor: Li, Xianjun; Wang, Junhuan; Wu, Wei; Jia, Yang; Fan, Shuanghu; Su Hlaing, Thet; Khokhar, Ibatsam; Yan, Yanchun.
Título: Cometabolic biodegradation of quizalofop-p-ethyl by Methylobacterium populi YC-XJ1 and identification of QPEH1 esterase
Fonte: Electron. j. biotechnol;46:38-49, jul. 2020. ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Basic Research Fund of Chinese Academy of Agricultural Sciences.
Resumo: BACKGROUND: Quizalofop-p-ethyl (QPE), a unitary R configuration aromatic oxyphenoxypropionic acid ester (AOPP) herbicide, was widely used and had led to detrimental environmental effects. For finding the QPEdegrading bacteria and promoting the biodegradation of QPE, a series of studies were carried out. RESULTS: A QPE-degrading bacterial strain YC-XJ1 was isolated from desert soil and identified as Methylobacterium populi, which could degrade QPE with methanol by cometabolism. Ninety-seven percent of QPE (50 mg/L) could be degraded within 72 h under optimum biodegradation condition of 35°C and pH 8.0. The maximum degradation rate of QPE was 1.4 mg/L/h, and the strain YC-XJ1 exhibited some certain salinity tolerance. Two novel metabolites, 2-hydroxy-6-chloroquinoxaline and quinoxaline, were found by high-performance liquid chromatography/mass spectroscopy analysis. The metabolic pathway of QPE was predicted. The catalytic efficiency of strain YC-XJ1 toward different AOPPs herbicides in descending order was as follows: haloxyfop-pmethyl ≈ diclofop-methyl ≈ fluazifop-p-butyl N clodinafop-propargyl N cyhalofop-butyl N quizalofop-p-ethyl N fenoxaprop-p-ethyl N propaquizafop N quizalofop-p-tefuryl. The genome of strain YC-XJ1 was sequenced using a combination of PacBio RS II and Illumina platforms. According to the annotation result, one α/ß hydrolase gene was selected and named qpeh1, for which QPE-degrading function has obtained validation. Based on the phylogenetic analysis and multiple sequence alignment with other QPE-degrading esterases reported previously, the QPEH1 was clustered with esterase family V. CONCLUSION: M. populi YC-XJ1 could degrade QPE with a novel pathway, and the qpeh1 gene was identified as one of QPE-degrading esterase gene.
Descritores: Propionatos/metabolismo
Quinoxalinas/metabolismo
Methylobacterium/metabolismo
-Microbiologia do Solo
Biodegradação Ambiental
Methylobacterium/enzimologia
Methylobacterium/genética
Análise de Sequência de Proteína
Esterases/análise
Esterases/metabolismo
Herbicidas
Hidrolases/análise
Hidrolases/metabolismo
Hidrólise
Responsável: CL1.1 - Biblioteca Central



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