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Id: biblio-951821
Autor: Azam, Mudsser; Jan, Arif Tasleem; Kumar, Ashutosh; Siddiqui, Kehkashan; Mondal, Aftab Hossain; Haq, Qazi Mohd. Rizwanul.
Título: Study of pandrug and heavy metal resistance among E. coli from anthropogenically influenced Delhi stretch of river Yamuna
Fonte: Braz. j. microbiol;49(3):471-480, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: Council of Scientific & Industrial Research.
Resumo: Abstract Escalating burden of antibiotic resistance that has reached new heights present a grave concern to mankind. As the problem is no longer confined to clinics, we hereby report identification of a pandrug resistant Escherichia coli isolate from heavily polluted Delhi stretch of river Yamuna, India. E. coli MRC11 was found sensitive only to tobramycin against 21 antibiotics tested, with minimum inhibitory concentration values >256 µg/mL for amoxicillin, carbenicillin, aztreonam, ceftazidime and cefotaxime. Addition of certain heavy metals at higher concentrations were ineffective in increasing susceptibility of E. coli MRC11 to antibiotics. Withstanding sub-optimal concentration of cefotaxime (10 µg/mL) and mercuric chloride (2 µg/mL), and also resistance to their combinatorial use, indicates better adaptability in heavily polluted environment through clustering and expression of resistance genes. Interestingly, E. coli MRC11 harbours two different variants of blaTEM (blaTEM-116 and blaTEM-1 with and without extended-spectrum activity, respectively), in addition to mer operon (merB, merP and merT) genes. Studies employing conjugation, confirmed localization of blaTEM-116, merP and merT genes on the conjugative plasmid. Understanding potentialities of such isolates will help in determining risk factors attributing pandrug resistance and strengthening strategic development of new and effective antimicrobial agents.
Descritores: Metais Pesados/farmacologia
Farmacorresistência Bacteriana Múltipla
Rios/microbiologia
Escherichia coli/efeitos dos fármacos
Antibacterianos/farmacologia
-Óperon
beta-Lactamases/genética
beta-Lactamases/metabolismo
Testes de Sensibilidade Microbiana
Proteínas de Escherichia coli/genética
Proteínas de Escherichia coli/metabolismo
Escherichia coli/isolamento & purificação
Escherichia coli/enzimologia
Escherichia coli/genética
Índia
Responsável: BR1.1 - BIREME


  2 / 298 LILACS  
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Id: lil-788968
Autor: Chand, Karam; Biswas, Sanchay Kumar; Sharma, Gaurav; Saxena, Arpit; Tewari, Neha; Mahajan, Sonalika; Pandey, Awadh Bihari.
Título: Full genome sequencing of the bluetongue virus-1 isolate MKD20/08/Ind from goat in India
Fonte: Braz. j. microbiol;47(3):527-528, July-Sept. 2016.
Idioma: en.
Resumo: ABSTRACT This communication reports full genome sequencing of the bluetongue virus-1 (BTV-1) isolate MKD20/08/Ind from goat in northern India. The total BTV-1 genome size was found to be 19,190 bp. A comparison study between the Indian isolate and other global isolates revealed that it belongs to the 'Eastern' BTV topotype. The full genome sequence of BTV-1 will provide vital information on its geographical origin and it will also be proved useful for comparing the Indian isolate with global isolates from other host species.
Descritores: Cabras/virologia
Genoma Viral
Análise de Sequência de DNA
Vírus Bluetongue/genética
-Filogenia
Vírus Bluetongue/isolamento & purificação
Vírus Bluetongue/classificação
Genômica
Sequenciamento de Nucleotídeos em Larga Escala
Sorogrupo
Índia
Limites: Animais
Responsável: BR1.1 - BIREME


  3 / 298 LILACS  
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Id: lil-780820
Autor: Ramya, Shanivarsanthe Leelesh; Venkatesan, Thiruvengadam; Srinivasa Murthy, Kottilingam; Jalali, Sushil Kumar; Verghese, Abraham.
Título: Detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from diamondback moth, Plutella xylostella (Linnaeus), from India and its possible role in indoxacarb degradation
Fonte: Braz. j. microbiol;47(2):327-336, Apr.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract Diamondback moth (DBM), Plutella xylostella (Linnaeus), is a notorious pest of brassica crops worldwide and is resistant to all groups of insecticides. The insect system harbors diverse groups of microbiota, which in turn helps in enzymatic degradation of xenobiotic-like insecticides. The present study aimed to determine the diversity of gut microflora in DBM, quantify esterase activity and elucidate their possible role in degradation of indoxacarb. We screened 11 geographic populations of DBM in India and analyzed them for bacterial diversity. The culturable gut bacterial flora underwent molecular characterization with 16S rRNA. We obtained 25 bacterial isolates from larvae (n = 13) and adults (n = 12) of DBM. In larval gut isolates, gammaproteobacteria was the most abundant (76%), followed by bacilli (15.4%). Molecular characterization placed adult gut bacterial strains into three major classes based on abundance: gammaproteobacteria (66%), bacilli (16.7%) and flavobacteria (16.7%). Esterase activity from 19 gut bacterial isolates ranged from 0.072 to 2.32 µmol/min/mg protein. Esterase bands were observed in 15 bacterial strains and the banding pattern differed in Bacillus cereus – KC985225 and Pantoea agglomerans – KC985229. The bands were characterized as carboxylesterase with profenofos used as an inhibitor. Minimal media study showed that B. cereus degraded indoxacarb up to 20%, so it could use indoxacarb for metabolism and growth. Furthermore, esterase activity was greater with minimal media than control media: 1.87 versus 0.26 µmol/min/mg protein. Apart from the insect esterases, bacterial carboxylesterase may aid in the degradation of insecticides in DBM.
Descritores: Oxazinas/metabolismo
Bactérias/enzimologia
Carboxilesterase/metabolismo
Esterases/metabolismo
Microbioma Gastrointestinal
Inseticidas/metabolismo
Mariposas/microbiologia
-Filogenia
Bactérias/isolamento & purificação
Bactérias/classificação
Bactérias/genética
Trato Gastrointestinal/microbiologia
Carboxilesterase/genética
Esterases/genética
Índia
Limites: Animais
Masculino
Feminino
Responsável: BR1.1 - BIREME


  4 / 298 LILACS  
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Id: biblio-839391
Autor: Patil, Virupaksh U; Girimalla, Vanishree; Sagar, Vinay; Chauhan, Rajinder Singh; Chakrabarti, Swarup Kumar.
Título: Genome sequencing of four strains of Phylotype I, II and IV of Ralstonia solanacearum that cause potato bacterial wilt in India
Fonte: Braz. j. microbiol;48(2):193-195, April.-June 2017. tab.
Idioma: en.
Resumo: Abstract Ralstonia solanacearum is a heterogeneous species complex causing bacterial wilts in more than 450 plant species distributed in 54 families. The complexity of the genome and the wide diversity existing within the species has led to the concept of R. solanacearum species complex (RsSC). Here we report the genome sequence of the four strains (RS2, RS25, RS48 and RS75) belonging to three of the four phylotypes of R. solanacearum that cause potato bacterial wilt in India. The genome sequence data would be a valuable resource for the evolutionary, epidemiological studies and quarantine of this phytopathogen.
Descritores: Doenças das Plantas/microbiologia
Solanum tuberosum/microbiologia
DNA Bacteriano/química
Genoma Bacteriano
Análise de Sequência de DNA
Ralstonia solanacearum/genética
Genótipo
-DNA Bacteriano/genética
Ralstonia solanacearum/isolamento & purificação
Ralstonia solanacearum/classificação
Índia
Responsável: BR1.1 - BIREME


  5 / 298 LILACS  
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Id: lil-767063
Autor: Phadke, Vandana; Makhija, Meena; Singh, Harpreet.
Título: The use of evidence-based practices for the management of shoulder impingement syndrome among Indian physical therapists: a cross-sectional survey
Fonte: Braz. j. phys. ther. (Impr.) = Rev. bras. fisioter;19(6):473-481, Nov.-Dec. 2015. tab, graf.
Idioma: en.
Resumo: ABSTRACT Background: The understanding of the pathomechanics of shoulder impingement has evolved over the years. Likewise, assessment techniques and effective treatment strategies have also been developed. Physical therapists should keep up-to-date on the current evidence. Objective: This study explored the practices currently used by Indian physical therapists for the assessment and management of shoulder impingement syndrome (SIS). Method: Using an online questionnaire, therapists were asked to declare the causes, methods of assessment and their choices of physical therapy techniques for the management of SIS. The proportions of therapists using different techniques were analyzed descriptively, and comparisons across gender, experience level, and training were made. Data were analyzed to see if the choices of respondents compared with their responses for etiology. Results: A total of 211 responses were analyzed. Most respondents (>75%) believed that overuse and abnormal motion/posture are the most significant causes of SIS. However, fewer respondents reported assessing posture (60.2%) and dyskinesis, especially in women (24.2%). Ninety-four percent of the respondents reported using exercises, but exercise prescription was rather generic. Therapists additionally trained in the techniques of joint mobilization or taping declared using these techniques more frequently. The use of interferential therapy and ultrasound was reported by 89.5% and 98.4% of respondents, respectively Conclusion: Most therapists declared awareness of current recommended practices, but patient assessment, exercise prescription, and use of electrotherapy modalities were only partially based on current evidence. The study helps to identify gaps in current physical therapy approaches to SIS in India.
Descritores: Modalidades de Fisioterapia
Síndrome de Colisão do Ombro
Índia
-Resultado do Tratamento
Fisioterapeutas
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-975617
Autor: Chitguppi, Chandala; Brar, Tripti.
Título: Do Otolaryngology Patients Show Gender Preference When Choosing a Surgeon? — A Quantitative and Qualitative Analysis
Fonte: Int. arch. otorhinolaryngol. (Impr.);22(4):404-407, Oct.-Dec. 2018. tab.
Idioma: en.
Resumo: Abstract Introduction Otorhinolaryngology has always been considered a gender-neutral specialty, whereas in several other specialties, such as obstetrics, gynecology and urology, gender preference has been consistently shown by patients when choosing their treating surgeon. To date, no study has been performed to analyze whether this practice of gender preference is prevalent in otorhinolaryngology patients too. Objectives To identify if gender preference exists in the field of otorhinolaryngology, specifically in its four subspecialties, namely otology, pediatric otolaryngology, laryngology and head and neck surgery. Methods Patients attending our outpatient department were asked to complete a preformed proforma. The pro forma consisted of two parts, demographic details of the subjects and gender preference in the following subspecialties: otology, pediatric otolaryngology, laryngology and head and neck oncosurgery. Results A total of 1,112 subjects took part in the study, out of which 1,089 subjects were included in the final analysis. Female gender preference was highest in the field of pediatric otolaryngology, while male preference was highest for head and neck oncosurgery. Conclusion Though otorhinolaryngology and head and neck surgery has been considered a gender-neutral field, subspecialties of this field show considerable gender preference.
Descritores: Procedimentos Cirúrgicos Otorrinolaringológicos
Preferência do Paciente/estatística & dados numéricos
Sexismo
-Otolaringologia
Otorrinolaringopatias/cirurgia
Assistência Centrada no Paciente
Cirurgiões
Índia
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Idoso
Responsável: BR66.1 - Divisão de Biblioteca e Documentação


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Id: biblio-898909
Autor: Nongbri, Sandor Rudy L; Verma, Henu Kumar; Lakkakula, Bhaskar V. K. S; Patra, Pradeep Kumar.
Título: Presence of atypical beta globin (HBB) gene cluster haplotypes in sickle cell anemia patients of India
Fonte: Rev. bras. hematol. hemoter;39(2):180-182, Apr.-June 2017. tab.
Idioma: en.
Projeto: Chhattisgarh Council of Science & Technology.
Descritores: Haplótipos
Família Multigênica
Globinas beta
Índia
Anemia Falciforme
Limites: Seres Humanos
Tipo de Publ: Carta
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: biblio-889185
Autor: Muthukamalam, Santhakumar; Sivagangavathi, Sivalingam; Dhrishya, Dharmapal; Sudha Rani, Sadras.
Título: Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria
Fonte: Braz. j. microbiol;48(4):637-647, Oct.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: UGC.
Resumo: ABSTRACT Role of microbes in bioremediation of oil spills has become inevitable owing to their eco friendly nature. This study focused on the isolation and characterization of bacterial strains with superior oil degrading potential from crude-oil contaminated soil. Three such bacterial strains were selected and subsequently identified by 16S rRNA gene sequence analysis as Corynebacterium aurimucosum, Acinetobacter baumannii and Microbacterium hydrocarbonoxydans respectively. The specific activity of catechol 1,2 dioxygenase (C12O) and catechol 2,3 dioxygenase (C23O) was determined in these three strains wherein the activity of C12O was more than that of C23O. Among the three strains, Microbacterium hydrocarbonoxydans exhibited superior crude oil degrading ability as evidenced by its superior growth rate in crude oil enriched medium and enhanced activity of dioxygenases. Also degradation of total petroleum hydrocarbon (TPH) in crude oil was higher with Microbacterium hydrocarbonoxydans. The three strains also produced biosurfactants of glycolipid nature as indicated d by biochemical, FTIR and GCMS analysis. These findings emphasize that such bacterial strains with superior oil degrading capacity may find their potential application in bioremediation of oil spills and conservation of marine and soil ecosystem.
Descritores: Poluentes do Solo/metabolismo
Tensoativos/metabolismo
Proteínas de Bactérias/metabolismo
Petróleo/microbiologia
Actinobacteria/metabolismo
Corynebacterium/metabolismo
Acinetobacter baumannii/metabolismo
Dioxigenases/metabolismo
-Filogenia
Microbiologia do Solo
Tensoativos/química
Proteínas de Bactérias/genética
Biodegradação Ambiental
Petróleo/análise
Poluição por Petróleo/análise
Actinobacteria/crescimento & desenvolvimento
Actinobacteria/enzimologia
Actinobacteria/genética
Corynebacterium/crescimento & desenvolvimento
Corynebacterium/enzimologia
Corynebacterium/genética
Acinetobacter baumannii/crescimento & desenvolvimento
Acinetobacter baumannii/enzimologia
Acinetobacter baumannii/genética
Dioxigenases/genética
Índia
Responsável: BR1.1 - BIREME


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Id: biblio-1039053
Autor: Mondal, Chiranjit; Chandra, Amar Kumar.
Título: Goitrogenic/antithyroidal potential of moringa leaves (Moringa oleifera) and spinach (Spinacia oleracea) of Indian origin on thyroid status in male albino rats
Fonte: Braz. J. Pharm. Sci. (Online);55:e18005, 2019. tab, graf.
Idioma: en.
Resumo: Moringa leaves and spinach used as vegetables containing cyanogens and polyphenols having suspected antithyroidal activity; however, detailed studies in this aspect found unavailable. Goitrogenic/antithyroid potentiality of those plant foods of Indian origin was evaluated. To explore the goitrogenic/antithyroid effect of those plant foods cyanogenic glucosides, glucosinolates, thiocyanate and polyphenol content were measured. These plant foods were fed with diet regularly to adult male albino rats for 30 and 60 days respectively, followed by evaluation of morphological and functional status of thyroid,as thyroid gland weight, the activity status of thyroid hormone synthesizing enzymes viz. TPO, Na+-K+-ATPase, deiodinase I, thyroid hormone profiles, thyroid histoarchitecture as well as urinary iodine and thiocyanate. In moringa leaves and spinach fed diet, there was a significant increase in urinary excretion of thiocyanate and iodine, enlargement of thyroid gland along with hypertrophy of thyroid follicles and altered in the activities of thyroid hormone synthesizing enzymes with concomitant changes of thyroid hormone profiles. Effects were more severe in moringa leaves treated group than that of spinach. Biochemical analysis moringa leaves and spinach reveal that those are rich sources of goitrogens and their prolonged consumption gradually developed a relative state of biochemical and morphological hypothyroidism.
Descritores: Antitireóideos/análise
Moringa oleifera/efeitos adversos
-Spinacia oleracea/efeitos adversos
Hipotireoidismo
Índia/etnologia
Limites: Animais
Masculino
Ratos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


  10 / 298 LILACS  
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Id: biblio-880175
Autor: Telles, Shirley.
Título: Yoga research and therapy in India
Fonte: Rev. bras. med. fam. comunidade;7(Suplemento 1):8-8, jun. 2012.
Idioma: en.
Resumo: Yoga was originally intended for spiritual growth. However, nowadays there is an increasing trend to use yoga as an add-on-therapy. In India it is believed that all diseases arise as a result of conflict between our instinct and our intellect1. This is also important in using yoga to promote positive health and prevent disease. A number of examples are cited here. Healthy children were given yoga and physical therapy and their physical fitness, cognitive functiones and emotional well being were tested. Yoga improved their emotional well being. Yoga also helped children with Duchenne muscular dystrophy (a degenerative disease) by improving their quality of life and mental status2. Again the benefits were ascribed to the mental as well as the physical effects of yoga. Of course yoga has marked benefits in healthy obese adults to prevent diseases by increasing mental well being, reducing stress and improving sleep3. Yoga also reduced anthropometric indices and brought about changes in leptin and adiponectin levels in otherwise healthy adults4a,b. This change too, was believed to be related to mental changes, along with the physical. Finally yoga can help in various disorders in which a person feels pain and distress5a,b. Many of the effects are believed to be due to changes in the functions at the level of the cortex and thalamus6, as well as the autonomic nervous system7. However the mechanisms underlying the effects of yoga need to be explored more thoroughly.
Descritores: Ioga
Ioga/história
Terapias Complementares
Promoção da Saúde
Índia
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM



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