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Id: biblio-951804
Autor: Bakhshi, Bita; Afshari, Nasim; Fallah, Fatemeh.
Título: Enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis as a reliable evidence for suspected Shigella spp. outbreaks
Fonte: Braz. j. microbiol;49(3):529-533, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Background Shigellosis remains a serious public health problem and an important cause of morbidity and mortality worldwide. The aim of this study was to characterize fliC and the genetic relatedness of Shigella spp. isolated during a one-year period from children in a suspected outbreak in Tehran, Iran. Methods and results Fifty Shigella spp. were isolated from 3779 stool samples of children with diarrhea (prevalence rate: 1.32%). Among the isolates, 92% were characterized as Shigella sonnei, while 6% and 2% were identified as S. flexneri and S. boydii, respectively. S. dysenteriae was not recovered from the patients. All isolates were negative for fliC except for Shigella standard strains. The enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) profiles allowed differentiating the 50 isolates into 5 ERIC types, which were grouped into five clusters (ET1-ET5). Computer-assisted clustering of the strains showed a high degree of similarity among the isolates. Conclusion In conclusion, given the clonal correlation of the Shigella strains isolated in this study and the lack of fliC among them, we propose that probably a single or limited fliC-defected Shigella clone spread and caused the outbreak.
Descritores: Shigella/isolamento & purificação
Surtos de Doenças
DNA Intergênico/genética
Disenteria Bacilar/microbiologia
-Filogenia
Shigella/classificação
Shigella/genética
DNA Bacteriano/genética
Reação em Cadeia da Polimerase
Disenteria Bacilar/epidemiologia
Flagelina/genética
Irã (Geográfico)/epidemiologia
Limites: Seres Humanos
Masculino
Feminino
Pré-Escolar
Criança
Responsável: BR1.1 - BIREME


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Id: biblio-1038308
Autor: Ataei, Mitra; Behfarjam, Farinaz; Jadali, Zohreh.
Título: TIM-3 genetic variants and risk of Behçet disease in the Iranian population
Fonte: An. bras. dermatol;94(4):429-433, July-Aug. 2019. tab.
Idioma: en.
Resumo: Abstract: Background: Behçet disease is a prototypical systemic autoimmune disease, caused by a complex interplay between environmental and genetic factors. The transmembrane immunoglobulin and mucin domain-3 (TIM-3) is a distinct member of the TIM family that is preferentially expressed on Th1 cells and plays a role in Th1-mediated autoimmune or inflammatory diseases, such as Behçet disease. Objective: The aim of this study was to test the potential association between TIM-3 gene polymorphisms and Behçet disease. Methods: Two single-nucleotide polymorphisms of TIM-3 (rs9313439 and rs10515746) were genotyped in 212 patients with Behçet disease and 200 healthy controls. Typing of the polymorphisms was performed using multiplex PCR amplification. Results: There were no significant differences in allele and genotype frequencies between the Behçet disease patients and controls who were successfully genotyped. Similar results were also found after stratification by gender, age, or clinical features. Study limitations: Lack of studies on various racial or ethnic groups and small sample size. Conclusion: This study failed to demonstrate any association between the tested TIM-3 polymorphisms and Behçet disease.
Descritores: Síndrome de Behçet/genética
Polimorfismo de Nucleotídeo Único
Receptor Celular 2 do Vírus da Hepatite A/genética
-Estudos de Casos e Controles
Modelos Logísticos
Fatores de Risco
Medição de Risco
Alelos
Estudos de Associação Genética
Reação em Cadeia da Polimerase Multiplex
Frequência do Gene
Irã (Geográfico)
Limites: Seres Humanos
Masculino
Feminino
Adulto
Responsável: BR1.1 - BIREME


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Id: biblio-828207
Autor: Nouri, Roghayeh; Ahangarzadeh Rezaee, Mohammad; Hasani, Alka; Aghazadeh, Mohammad; Asgharzadeh, Mohammad.
Título: The role of gyrA and parC mutations in fluoroquinolones-resistant Pseudomonas aeruginosa isolates from Iran
Fonte: Braz. j. microbiol;47(4):925-930, Oct.-Dec. 2016. tab.
Idioma: en.
Projeto: Infectious and Tropical Diseases Research Center.
Resumo: Abstract The aim of this study was to examine mutations in the quinolone-resistance-determining region (QRDR) of gyrA and parC genes in Pseudomonas aeruginosa isolates. A total of 100 clinical P. aeruginosa isolates were collected from different university-affiliated hospitals in Tabriz, Iran. Minimum inhibitory concentrations (MICs) of ciprofloxacin and levofloxacin were evaluated by agar dilution assay. DNA sequences of the QRDR of gyrA and parC were determined by the dideoxy chain termination method. Of the total 100 isolates, 64 were resistant to ciprofloxacin. No amino acid alterations were detected in gyrA or parC genes of the ciprofloxacin susceptible or ciprofloxacin intermediate isolates. Thr-83 → Ile substitution in gyrA was found in all 64 ciprofloxacin resistant isolates. Forty-four (68.75%) of them had additional substitution in parC. A correlation was found between the number of the amino acid alterations in the QRDR of gyrA and parC and the level of ciprofloxacin and levofloxacin resistance of the P. aeruginosa isolates. Ala-88 → Pro alteration in parC was generally found in high level ciprofloxacin resistant isolates, which were suggested to be responsible for fluoroquinolone resistance. These findings showed that in P. aeruginosa, gyrA was the primary target for fluoroquinolone and additional mutation in parC led to highly resistant isolates.
Descritores: Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/genética
Infecções por Pseudomonas/microbiologia
Infecções por Pseudomonas/epidemiologia
Fluoroquinolonas/farmacologia
DNA Girase/genética
DNA Topoisomerase IV/genética
Farmacorresistência Bacteriana
Mutação
-Pseudomonas aeruginosa/isolamento & purificação
Testes de Sensibilidade Microbiana
Análise de Sequência de DNA
Irã (Geográfico)/epidemiologia
Antibacterianos/farmacologia
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-788959
Autor: Bialvaei, Abed Zahedi; Kafil, Hossein Samadi; Asgharzadeh, Mohammad; Aghazadeh, Mohammad; Yousefi, Mehdi.
Título: CTX-M extended-spectrum ß-lactamase-producing Klebsiella spp, Salmonella spp, Shigella spp and Escherichia coli isolates in Iranian hospitals
Fonte: Braz. j. microbiol;47(3):706-711, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: Abstract This study was conducted in Iran in order to assess the distribution of CTX-M type ESBLs producing Enterobacteriaceae. From January 2012 to December 2013, totally 198 E. coli, 139 Klebsiella spp, 54 Salmonella spp and 52 Shigella spp from seven hospitals of six provinces in Iran were screened for resistance to extended-spectrum cephalosporins. After identification and susceptibility testing, isolates presenting multiple-drug resistance (MDR) were evaluated for ESBL production by the disk combination method and by Etest using (cefotaxime and cefotaxime plus clavulanic acid). All isolates were also screened for bla CTX-M using conventional PCR. A total of 42.92%, 33.81%, 14.81% and 7.69% of the E. coli, Klebsiella spp, Salmonella spp and Shigella spp isolates were MDR, respectively. The presence of CTX-M enzyme among ESBL-producing isolates was 85.18%, 77.7%, 50%, and 66.7%, in E. coli, Klebsiella spp, Salmonella spp and Shigella spp respectively. The overall presence of CTX-M genes in Enterobacteriaceae was 15.4% and among the resistant isolates was 47.6%. This study indicated that resistance to β-lactams mediated by CTX-M enzymes in Iran had similar pattern as in other parts of the world. In order to control the spread of resistance, comprehensive studies and programs are needed.
Descritores: Salmonella/enzimologia
Shigella/enzimologia
beta-Lactamases/metabolismo
Infecção Hospitalar
Infecções por Enterobacteriaceae/microbiologia
Infecções por Enterobacteriaceae/epidemiologia
Escherichia coli/enzimologia
Klebsiella/enzimologia
-Salmonella/efeitos dos fármacos
Shigella/efeitos dos fármacos
beta-Lactamases/genética
Testes de Sensibilidade Microbiana
Estudos Transversais
Escherichia coli/efeitos dos fármacos
Irã (Geográfico)/epidemiologia
Klebsiella/efeitos dos fármacos
Antibacterianos/farmacologia
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-893673
Autor: Rafighdoost, Houshang; Hashemi, Mohammad; Danesh, Hiva; Bizhani, Fatemeh; Bahari, Gholamreza; Taheri, Mohsen.
Título: Association of single nucleotide polymorphisms in AXIN2, BMP4, and IRF6 with Non-Syndromic Cleft Lip with or without Cleft Palate in a sample of the southeast Iranian population
Fonte: J. appl. oral sci;25(6):650-656, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: Zahedan University of Medical Sciences.
Resumo: Abstract Non-syndromic cleft lip with or without palate (NSCL/P) is a common congenital malformation worldwide, with complex etiology. It has been proposed that interaction of genes and environmental factors play a role in the predisposition to this disease. Objectives: The aim of this study was to examine the association between AXIN2 (axis inhibition protein 2) rs7224837, BMP4 (bone morphogenetic protein 4) rs17563, and IRF6 (interferon regulatory factor 6) rs861019 and 2235371 polymorphisms and NSCL/P in an Iranian population. Material and Methods: This case-control study was carried out on 132 unrelated NSCL/P patients and 156 healthy subjects. The variants were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The findings suggest that BMP4 rs17563 polymorphism significantly decreased the risk of NSCL/P in codominant (OR=0.36, 95%CI=0.17-0.79, p=0.012, CT vs CC and OR=0.11, 95%CI=0.01-0.88, p = 0.019, TT vs CC), dominant (OR=0.30, 95%CI=0.15-0.62, p = 0.0007, CT+TT vs CC), recessive (OR=0.12, 95%CI=0.02-0.99, p = 0.023, TT vs CC+CT), overdominant (OR=0.39, 95%CI = 0.18-0.84, p=0.021, CT vs CC+TT), and allele (OR=0.28, 95%CI=0.15-0.55, p<0.0001, T vs C) inheritance models. Our findings did not support an association between AXIN2 rs7224837 and IRF6 rs861019 polymorphism and risk/protection of NSCL/P. The IRF6 2235371 variant was not polymorphic in our population. Conclusion: The results indicate that the BMP4 rs17563 variant is likely to confer a protective effect against the occurrence of NSCL/P in a sample of the southeast Iranian population.
Descritores: Fenda Labial/genética
Fissura Palatina/genética
Fatores Reguladores de Interferon/genética
Proteína Morfogenética Óssea 4/genética
Proteína Axina/genética
-Polimorfismo de Fragmento de Restrição
Estudos de Casos e Controles
Predisposição Genética para Doença
Polimorfismo de Nucleotídeo Único
Frequência do Gene
Genótipo
Irã (Geográfico)
Limites: Seres Humanos
Masculino
Feminino
Criança
Responsável: BR1.1 - BIREME


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Id: biblio-1012597
Autor: Hosseini, Raheleh Sadat; Mansour-Ghanaei, Fariborz; Shafaghi, Afshin; Hojati, Amineh; Joukar, Farahnaz; Roushan, Zahra Atrkar; Hosseini, Fakhri Alsadat; Mavaddati, Sara.
Título: Exacerbation causes among inflammatory bowel disease patients in Guilan Province north of Iran / Causas de exacerbação entre pacientes com doença inflamatória intestinal na província de Guilan, norte do Irã
Fonte: J. coloproctol. (Rio J., Impr.);39(2):138-144, Apr.-June 2019. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective: Numerous factors may contribute as triggers to the exacerbation of the condition of patients with inflammatory bowel disease. Methods: The medical files of 109 patients with the positive history of inflammatory bowel disease exacerbation between March 2016 and March 2017 were assessed retrospectively. Data were obtained using the inflammatory bowel disease data bank software. The parameters were obtained from the inflammatory bowel disease data bank software. The mentioned parameters were assessed in terms of type and severity of disease using chi-square test in SPSS software. Moreover, binary logistic regression test was used to assess the associations between season of disease onset and inflammatory bowel disease exacerbation as odds ratios with 95% confidence intervals (95% CI). Results: Overall, (88.1%) of cases with inflammatory bowel disease exacerbation, had ulcerative colitis. The mean age of patients was 38.14 ± 14.66 years. The disease duration in all patients (ulcerative colitis and Crohn's disease) was 35.43 and 38.85 months, respectively. About 50% of patients with infection were strongyloides stercoralis positive. The occurrence of mild inflammatory bowel disease exacerbation was significantly higher in spring in comparison to other seasons (OR = 3.58; 95% CI 0.1-1.04). Most patients with ulcerative colitis were prescribed salicylates alone (53.12%). Most patients with Crohn's disease with mild and severe activity were non-smokers (p = 0.058). This difference was marginally significant. Conclusion: It is suggested that in future studies, the evidences of distribution of SS infections among patients with inflammatory bowel disease and the history of exacerbation along with other environmental factors such as enhancing nutritional quality and surface water be taken into consideration.

RESUMO Objetivo: Em pacientes com doença inflamatória intestinal, vários fatores podem servir como gatilhos para a exacerbação do quadro. Métodos: Os prontuários de 109 pacientes com história de exacerbação da doença inflamatória intestinal entre março de 2016 e março de 2017 foram avaliados retrospectivamente. Os dados foram obtidos usando o software do banco de dados sobre doença inflamatória intestinal, que também foi usado para a definição dos parâmetros do estudo. Esses parâmetros foram avaliados quanto ao tipo e severidade da doença usando o teste do qui-quadrado no software SPSS. Além disso, o teste de regressão logística binária foi utilizado para avaliar as associações entre a estação do início da doença e a exacerbação da doença inflamatória intestinal, expressados em razão de probabilidade (odds ratio) com intervalos de confiança de 95% (95% CI). Resultados: No geral, 88,1% dos casos de exacerbação da doença inflamatória intestinal foram observados em pacientes com colite ulcerativa. A média de idade dos pacientes foi de 38,14 ± 14,66 anos. Em todos os pacientes, a duração média da doença (colite ulcerativa e doença de Crohn) foi de 35,43 e 38,85 meses, respectivamente. Cerca de 50% dos casos de infecção apresentaram cultura positiva para Strongyloides stercoralis. A ocorrência de leve exacerbação da doença inflamatória intestinal foi significativamente maior na primavera em comparação com outras estações (OR = 3,58; 95% CI: 0,1-1,04). A maioria dos pacientes com colite ulcerativa foi medicada apenas com salicilatos (53,12%). A maioria dos pacientes com doença de Crohn com atividade classificada como leve ou grave era não fumante (p = 0,058). Essa diferença foi marginalmente significativa. Conclusão: Sugere-se que, em estudos futuros, as evidências de distribuição das infecções por Strongyloides stercoralis em pacientes com doença inflamatória intestinal e história de exacerbação sejam levadas em consideração em conjunto com outros fatores ambientais, como qualidade nutricional e da água de superfície.
Descritores: Doenças Inflamatórias Intestinais/etiologia
Colite Ulcerativa
Doença de Crohn
-Irã (Geográfico)
Limites: Seres Humanos
Masculino
Feminino
Adulto
Responsável: BR545.3 - Biblioteca ICBS


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Id: biblio-998270
Autor: Bahari, Mahmoud; Mohammadi, Narmin; Kimyai, Soodabeh; Kahnamoui, Mehdi Abed; Vahedpour, Hafez; Torkani, Mohammad Ali Mohammadi; Oskoee, Ayda Savadi.
Título: Effect of Different Fiber Reinforcement Strategies on the Fracture Strength of Composite Resin Restored Endodontically Treated Premolars
Fonte: Pesqui. bras. odontopediatria clín. integr;19(1):4465, 01 Fevereiro 2019. ilus, tab.
Idioma: en.
Resumo: Objective: To compare the effect of three different fiber reinforcement strategies on the fracture strength of composite resin restored endodontically treated premolars. Material and Methods: Seventy-two sound human premolars extracted for orthodontic reasons were divided into 6 groups (n=12) after endodontic treatment. Group 1: intact teeth (positive control); Group 2: endodontically treated teeth without restoration (negative control); Group 3: composite resin restoration; Group 4: placement of fibers at occlusal position; Group 5: splinting the buccal and palatal walls with horizontal fiber posts; Group 6: placement of fibers at the occlusal position after splinting the buccal and palatal walls with horizontal fiber posts. Then fracture strength was measured at a crosshead speed of 0.5 mm/min in a universal testing machine. Data were analyzed using one-way ANOVA and post hoc Tukey tests at α=0.05. Results: There were significant differences between the negative and positive control groups (p<0.001) and between the negative control group and all the other study groups (p<0.001). However, there were no statistically significant differences between the positive control group and all the experimental groups and between the experimental groups (p>0.05). Conclusion: Fiber insertion had no additional reinforcing effect on the fracture strength following composite resin restoration.
Descritores: Resistência à Tração
Dente Pré-Molar
Teste de Materiais
Resinas Compostas
Dente não Vital
-Análise de Variância
Irã (Geográfico)
Tipo de Publ: Estudo Comparativo
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Id: biblio-998246
Autor: Naghili, Armaghan; Yousefi, Navid; Zajkani, Elham; Ghasemi, Amir; Torabzadeh, Hassan.
Título: Influence of Cavity Dimensions on Microleakage of Two Bulk-Fill Composite Resins
Fonte: Pesqui. bras. odontopediatria clín. integr;19(1):4628, 01 Fevereiro 2019. tab.
Idioma: en.
Resumo: Objective: To evaluate the effect of cavity dimensions on the amount of microleakage in two different types of bulk-fill composite resins. Material and Methods: Forty class II cavities were prepared in the mesial and distal surfaces of human molars without any carious lesions. The samples were divided into 4 groups (n=10): Group 1: cavities with 3 mm of buccolingual width (known as the smaller cavity), filled with Tetric N-Ceram Bulk Fill composite resin; Group 2: cavities with 6 mm of buccolingual width (larger cavity), filled with Tetric N-Ceram Bulk Fill composite resin; Group 3: cavities with 3 mm of buccolingual width, filled with X-Tra Base composite resin; and Group 4: cavities with 6 mm of buccolingual width, filled with X-Tra Base composite. After the specimens were thermocycled for 500 cycles at 5/55°C, they were immersed in 1% methylene blue for 24 hours, and then cut into sections mesiodistally in the longitudinal axis of each tooth. Then, the samples were scored regarding the amount of dye penetration in two occlusal and gingival areas under a stereomicroscope (x32). Data was submitted to Kruskal- Wallis and Mann-Whitney tests. Results: The highest degrees of microleakage in larger cavities filled with X-Tra Base among the four groups. There was a significant statistic difference (p=0.012) between large and small cavities filled with X-Tra Base (Groups 3 and 4); however, there was no significant difference between the two cavity sizes of Tetric N-Ceram Bulk-filled groups. Conclusion: Microleakage of composite resins depends on the dimension of the cavity and the type of composite resin used.
Descritores: Resinas Compostas/química
Preparo da Cavidade Dentária
Infiltração Dentária/etiologia
-Estatísticas não Paramétricas
Materiais Dentários
Irã (Geográfico)
Tipo de Publ: Estudo Comparativo
Estudos de Avaliação
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Id: biblio-998244
Autor: Hashemipour, Maryam Alsadat; Navabi, Nader; Lotfi, Soudabeh; Sepehri, Gholamreza; Rastgarian, Arman.
Título: Pattern of Logical Drug Prescription Among Iranian General Dental Practitioners
Fonte: Pesqui. bras. odontopediatria clín. integr;19(1):4460, 01 Fevereiro 2019. tab.
Idioma: en.
Resumo: Objective: To determine the prescribing patterns of the general dental practitioners in Kerman province in Iran. Material and Methods: In this cross sectional study 1200 prescriptions, which were prescribed by general dentists in Kerman province during one-year period, were evaluated. Each prescription was assessed for the number of drugs per prescription, drug (category, name, route of administration, frequency) and duration of treatment. Descriptive statistics were used to calculate the absolute and relative frequencies, mean and standard deviation. The Chi-square test, analysis of variance (ANOVA) and t-test were used. The statistical tests were performed at the significance level of 0.05. Results: The mean number of drugs per prescription was 2.59. Antibiotics, Analgesics, corticosteroids and antiseptics were the most common drug category prescribed drugs by general dentists. Oral route was the commonest route of drug prescription (84.1%). Amoxicillin capsule (60.5%) was the commonest drug prescribed by general practitioners followed by ibuprofen derivatives (55.4%). Spelling errors was found in 62.7% of prescriptions. The mean score of prescriptions for logical prescription pattern was 7.36 ± 1.32 out of 9. Conclusion: Dental prescribing patterns should be considered as a potential area for improvement in the treatment process and patient safety. It is suggested to emphasis more on principles of prescription at university and retraining courses for dentists.
Descritores: Prescrições de Medicamentos
Odontalgia/etiologia
Conhecimentos, Atitudes e Prática em Saúde
Odontólogos
Irã (Geográfico)
-Distribuição de Qui-Quadrado
Estudos Transversais/métodos
Análise de Variância
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Id: biblio-998214
Autor: Eslami, Hosein; Faramarzi, Masoumeh; Majidi, Jafar; Bohlouli, Sepideh; Khani, Anahita Javad; Aghebati-Maleki, Leili; Motahari, Paria.
Título: Comparing the Levels of Gingival Crevicular Fluid Prostaglandin E2 in Generalized Chronic Periodontitis Between Healthy and Type 2 Diabetes Patients: A Case-Control Study
Fonte: Pesqui. bras. odontopediatria clín. integr;19(1):4523, 01 Fevereiro 2019. tab, graf.
Idioma: en.
Resumo: Objective: To compare the prostaglandin E2 (PGE2) levels of gingival crevicular fluid in generalized chronic periodontitis between healthy and type 2 diabetic patients. Material and Methods: 56 diabetic and non-diabetic participants with generalized chronic periodontitis were selected randomly. They were divided into two groups (G1: generalized chronic periodontitis patients with normal blood sugar; and G2: generalized chronic periodontitis patients with diabetes). Gingival crevicular fluid samples were obtained from both groups. The average of 2 samples per day were centrifuged in a laboratory at 2500 rpm and temperature of 4°C for 5 minutes and placed in a refrigerator at -20°C. The level of PGE2 was measured using ELISA and Abcam kit. Data were analyzed by Kolmogorov-Smirnov, Mann-Whitney U Test, Pearson and independent T tests. The significant amount was considered 0.05 in this test (α<0.05). Results: The mean level of PGE2 was significantly different in the two groups and the mean level of PGE2 in the control group was lower than the case group. There was no statistically significant relationship between PGE2 with pocket depth, fasting blood sugar (FBS) and HBA1C (p>0.05). Conclusion: PGE2 level of diabetic patient group with chronic generalized periodontitis was significantly more than non-diabetic group with generalized chronic periodontitis.
Descritores: Higiene Bucal
Doenças Periodontais
Estudos de Casos e Controles
Diabetes Mellitus
Periodontite Crônica/diagnóstico
-Estatísticas não Paramétricas
Irã (Geográfico)
Limites: Seres Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos



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