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Battistella, Linamara Rizzo
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Id: lil-704981
Autor: Tuacek, Tatiana Amadeo; Tsukimoto, Gracinda Rodrigues; Figliolia, Carmen Silvia; Cardoso, Maiara Celina de Carvalho; Tsukimoto, Denise Rodrigues; Rosa, Chennyfer Dobbins Paes; Imamura, Marta; Battistella, Linamara Rizzo.
Título: Neuropatias: Síndrome de Guillain-Barré: reabilitação / Neuropathies: Guillain-Barré syndrome: rehabilitation
Fonte: Acta fisiátrica;20(2), jun. 2013.
Idioma: pt.
Resumo: A estratégia de busca utilizada baseou-se em perguntas estruturadas na forma P.I.C.O. (das iniciais "Paciente", "Intervenção", "Controle", "Outcome"). Foram utilizados como descritores: Exercise, Physical OR Physical Exercise OR Physical Exercises OR prevention and control OR preventive therapy OR preventive measures OR prophylaxis OR preventions OR control OR Guillain-Barre Syndrome OR Syndrome, Guillain-Barre OR Guillaine-Barre Syndrome OR Guillaine Barre Syndrome OR Syndrome, Guillain Barre OR Polyneuropathy, Acute Inflammatory OR Acute Autoimmune Neuropathy OR Acute Autoimmune Neuropathies OR Plasmaphereses OR Plasmapheresis OR Plasma Exchange OR Immunoglobulins/therapeutic use OR Immunoglobulins/administration and dosage OR Exercise Therapy OR Physical Therapy OR Physical Therapies OR Exercise therapies OR Resistant Training OR Strength Training OR rehabilitation OR Splints OR Splint OR Orthopedic Fixation Devices OR Orthotic Devices OR device orthotic OR devices orthotic OR Orthoses OR Orthosis OR Upper Extremity OR Upper Extremities OR Upper Limb OR Upper limbs OR Membrum Superius OR Extremities, Upper OR Limb, Upper OR Limbs, Upper OR hand deformities OR Hand Deformities, acquired OR Rehabilitation OR Lower Extremity OR Extremities, Lower OR Lower Extremities OR Lower Limb OR Limb, Lower OR Lower Limbs OR Membrum Inferius OR Walking OR Ambulation OR dependent ambulation OR Ambulation, Dependent OR gait OR gaits OR Functional Electrical Stimulation OR Electrical Stimulation, Functional OR Fes OR Electric Stimulation Therapy OR Stimulation Therapy, Electric OR Therapeutic Electrical Stimulation OR Electrical Stimulation, Therapeutic OR Stimulation, Therapeutic Electrical OR Therapy, Electric Stimulation OR Therapeutic Electric Stimulation OR Electric Stimulation, Therapeutic OR Stimulation, Therapeutic Electric OR Electrical Stimulation Therapy OR Stimulation Therapy, Electrical OR Therapy, Electrical Stimulation OR Self-help devices OR Self-help device OR Device, Self-Help device OR Assistive Technology OR Assistive Technologies OR Technologies, Assistive OR Technology, Assistive OR Assistive Devices OR Assistive Device OR Device, Assistive OR Devices, Assistive OR daily activities OR daily activity OR activity of daily living OR activities of daily living OR activities of self care OR activity of self care OR usual activities OR usual activity OR usual activity of daily living OR usual activities of daily living OR Patient Positioning OR Patient Positionings OR Positioning, Patient OR Positionings, Patient OR pressure ulcer OR pressure ulcer/prevention & control OR Guillain-Barre OR Guillain-Barre Syndrome/therapy* OR Guillain-Barre Syndrome/rehabilitation* OR Nursing care OR Pressure Ulcer OR Pressure Ulcers OR Pressure Sore OR Pressure Sores OR Decubitus ulcer OR Bed sore OR Bedsore OR Weaning Mechanical Ventilation OR Extubation OR Mechanical Ventilation Predictors OR Weaning Mechanical Ventilator Predictors OR Extubation Predictors.

The search strategy used was based on structured questions in the P.I.C.O. format (from the initials: Patient, Intervention, Control and Outcome). The descriptors used were: Exercise, Physical OR Physical Exercise OR Physical Exercises OR prevention and control OR preventive therapy OR preventive measures OR prophylaxis OR preventions OR control OR Guillain-Barre Syndrome OR Syndrome, Guillain-Barre OR Guillaine-Barre Syndrome OR GuillaineBarre Syndrome OR Syndrome, GuillainBarre OR Polyneuropathy, Acute Inflammatory OR Acute Autoimmune Neuropathy OR Acute Autoimmune Neuropathies OR Plasmaphereses OR Plasmapheresis OR Plasma Exchange OR Immunoglobulins/therapeutic use OR Immunoglobulins/administration and dosage OR Exercise Therapy OR Physical Therapy OR Physical Therapies OR Exercise therapies OR Resistant Training OR Strength Training OR rehabilitation OR Splints OR Splint OR Orthopedic Fixation Devices OR Orthotic Devices OR device orthotic OR devices orthotic OR Orthoses OR Orthosis OR Upper Extremity OR Upper Extremities OR Upper Limb OR Upper limbs OR Membrum Superius OR Extremities, Upper OR Limb, Upper OR Limbs, Upper OR hand deformities OR Hand Deformities, acquired OR Rehabilitation OR Lower Extremity OR Extremities, Lower OR Lower Extremities OR Lower Limb OR Limb, Lower OR Lower Limbs OR Membrum Inferius OR Walking OR Ambulation OR dependent ambulation OR Ambulation, Dependent OR gait OR gaits OR Functional Electrical Stimulation OR Electrical Stimulation, Functional OR Fes OR Electric Stimulation Therapy OR Stimulation Therapy, Electric OR Therapeutic Electrical Stimulation OR Electrical Stimulation, Therapeutic OR Stimulation, Therapeutic Electrical OR Therapy, Electric Stimulation OR Therapeutic Electric Stimulation OR Electric Stimulation, Therapeutic OR Stimulation, Therapeutic Electric OR Electrical Stimulation Therapy OR Stimulation Therapy, Electrical OR Therapy, Electrical Stimulation OR Self-help devices OR Self-help device OR Device, Self-Help device OR Assistive Technology OR Assistive Technologies OR Technologies, Assistive OR Technology, Assistive OR Assistive Devices OR Assistive Device OR Device, Assistive OR Devices, Assistive OR daily activities OR daily activity OR activity of daily living OR activities of daily living OR activities of self care OR activity of self care OR usual activities OR usual activity OR usual activity of daily living OR usual activities of daily living OR Patient Positioning OR Patient Positionings OR Positioning, Patient OR Positionings, Patient OR pressure ulcer OR pressure ulcer/prevention & control OR Guillain-Barre OR Guillain-Barre Syndrome/therapy* OR Guillain-Barre Syndrome/rehabilitation* OR Nursing care OR Pressure Ulcer OR Pressure Ulcers OR Pressure Sore OR Pressure Sores OR Decubitus ulcer OR Bed sore OR Bedsore OR Weaning Mechanical Ventilation OR Extubation OR Mechanical Ventilation Predictors OR Weaning Mechanical Ventilator Predictors OR Extubation Predictors.
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: biblio-1012759
Autor: Filippo, Paula A. Di; Lannes, Saulo T; Meireles, Marcos A. D; Nogueira, Andressa F. S; Quirino, Célia R.
Título: Concentrations of acute-phase proteins and immunoglobulins in serum and synovial fluid in clinically healthy heifers and steers / Concentração de proteínas de fase aguda e de imunoglobulinas no soro e no líquido sinovial de bovinos clinicamente saudáveis
Fonte: Pesqui. vet. bras = Braz. j. vet. res;39(6):388-392, June 2019. tab.
Idioma: en.
Resumo: The aim of the study was to determine the concentration pattern of intra-articular acute phase proteins (APPs) and immunoglobulins in healthy crossbred cattle. Synovial fluid (SF) samples were collected from the radiocarpal joint of 25 heifers and 25 steers. Concentrations of APPs were measured by SDS-PAGE. The results were submitted to analysis of variance using the SAS statistical program, and means were compared by the Student-Newman-Keuls test (P<0.05). Thirty-seven proteins with molecular weights ranging from 7 to 37kDa were identified in SF of all animals. Eight were nominally identified with immunoglobulin A (IgA) and G (IgG), ceruloplasmin (Cp), transferrin (Tf), albumin (Ab), α1-antitripsin (AAT), α1-acid glycoprotein (AGP), and haptoglobin (Hp). The α1-antitripsin was only identified in the Sf of the heifers. The SF values of Cp, Hp, AGP and IgA were significantly higher in heifers than in steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, however the α1-antitrypsin was identified only in SF. The serum values Tf, AGP and IgG were significantly higher in heifers compared with steers. In conclusion, the physiological acute-phase proteins concentrations in synovial fluid of healthy ruminants can be useful in the interpretation of samples from animals with joint diseases. The SF electrophoretic profile of healthy ruminants differs depending on gender. Similar proteins were nominally identified in the sera, but only the SF of α1-antitrypsin.(AU)

O objetivo do estudo foi determinar o padrão de concentração de proteínas de fase aguda e de imunoglobulinas intra-articulares (APPs) em bovinos mestiços saudáveis. As amostras de fluido sinovial (SF) foram coletadas da articulação radiocárpica de 25 novilhas e 25 novilhos. As concentrações de APPs foram mensuradas por SDS-PAGE. Os resultados foram submetidos à análise de variância usando o programa estatístico SAS, e os meios foram comparados pelo teste Student-Newman-Keuls (P<0,05). Trinta e sete proteínas com pesos moleculares variando de 7 a 37kDa foram identificadas no SF de todos os animais. Oito foram nominalmente identificadas como imunoglobulina A (IgA) e G (IgG), ceruloplasmina (Cp), transferrina (Tf), albumina (Ab), a1-antitripsina (AAT), glicoproteína a1-ácido (AGP) e haptoglobina (Hp). A α1-antitripsina foi identificada apenas no SF das novilhas. Os valores de Cp, Hp, AGP e IgA no SF foram significativamente maiores em novilhas do que em novilhos. No soro, 34 proteínas com pesos moleculares entre 7 e 244kDa foram identificadas nas novilhas e novilhos. Proteínas similares foram identificadas nos soros, mas apenas o SF das novilhas apresentou a α1-antitripsina. Os valores séricos de Tf, AGP e IgG foram significativamente maiores em novilhas em relação aos novilhos. Conclui-se que a mensuração das concentrações das proteínas da fase aguda no líquido sinovial de animais saudáveis pode ser útil na avaliação de amostras oriundas de bovinos com afecções articulares. O perfil eletroforético do SF de ruminantes saudáveis difere em função do gênero e as diferenças devem ser levadas em consideração na interpretação dos achados.(AU)
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1005300
Autor: Montaño E, Dolly E; Alvarado R, Moisés G; Burgos Q, Maritza J.
Título: Validación del método de formación de coágulo para la determinación de endotoxinas bacterianas en inmunoglobulinas (IgG) nacionales / Method validation clot formation for determining bacterial endotoxins national immunoglobulin (IgG)
Fonte: Rev. Inst. Nac. Hig;47(1-2):41-48, 2016. graf, tab.
Idioma: es.
Resumo: Las endotoxinas bacterianas son lipopolisacáridos (LPS) localizados exclusivamente en la membrana externa de las bacterias gramnegativas, su ingreso al organismo a través de productos parenterales puede causar fiebre, taquicardia, aumento de presión sanguínea y en algunos casos ocasiona la muerte. Existen normativas internacionales acerca del límite de endotoxina para productos farmacéuticos inyectables, tal como las inmunoglobulinas (IgG), que pueden estar expuestas a contaminación durante el proceso de producción y por lo tanto es necesario realizar pruebas para la determinación de endotoxinas bacterianas. El método del lisado de amebocitos de Limulus (LAL) es una de ellas.Este método se fundamenta en la reacción del LAL, el cual es un extracto de células sanguíneas que interaccionan con endotoxinas, activando la cascada del proceso de coagulación y originando la formación de la coagulina. En diversas ocasiones algunas proteínas intervienen en la activación o desactivación de esta cascada, bien sea potenciando o inhibiendo la formación del coágulo. En el caso de la potenciación, el calentamiento es uno de los métodos recomendados por la farmacopea estadounidense (USP) para eliminar interferencias, puesto que desnaturaliza las proteínas que causan la potenciación sin pérdida de endotoxinas. En este trabajo se validó la determinación de endotoxinas bacterianas en IgG mediante el método LAL, el cual es un método rápido y de fácilejecución, por lo que puede implementarse como ensayo de rutina en control de calidad y por ende nos permite agilizar las Liberaciones de Lotes de estos productos.

Bacterial endotoxins are lipopolysaccharides (LPS) located exclusively in the outer membrane of gram-negative bacteria, their entry into the body through parenteral products can cause fever, tachycardia, increased blood pressure and in some cases cause death. There are international standards for endotoxin limit for injectable pharmaceuticals, such as immunoglobulins (IgG), which may be exposed to contamination during the production process and therefore it is necessary to test for determination of bacterial endotoxins. The method of the Limulus amebocyte lysate (LAL) is one of them. This method is based on the LAL reaction, which is an extract of blood cells which interact with endotoxin, triggering the cascade of the coagulation process and causing the formation of coagulin. On several occasions some proteins involved in the activation or deactivation of this waterfall, either by enhancing or inhibiting clot formation. In the case of empowerment, the warming is one of those recommended by the US Pharmacopoeia (USP) to eliminate interference, since denatures proteins that cause endotoxin enhancement lossless methods. In this paper the determination of bacterial endotoxins in IgG was standardized by the LAL method, which is quick and easy to implement method, which can be implemented as a routine test in quality control and thus allows us to streamline releases Lots of these products.
Responsável: VE9.1 - Biblioteca


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Texto completo SciELO Brasil
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Id: lil-623604
Autor: Sadigursky, Moysés; Von Kreuter, Betsy F; Santos-Buch, Charles A.
Título: Development of Chagasic autoimmune myocarditis associated with anti-idiotype reaction
Fonte: Mem. Inst. Oswaldo Cruz;83(supl.1):363-366, Nov. 1988. graf.
Idioma: en.
Conferência: Apresentado em: Annual Meeting on Basic Research in Chagas's disease, 15, Apresentado em: Meeting of the Brazilian Society of Protozoology4, Caxambu, 7-10 Nov. 1988.
Responsável: BR1.1 - BIREME


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Id: biblio-954857
Autor: Squaiella-Baptistão, Carla Cristina; Magnoli, Fábio Carlos; Marcelino, José Roberto; Sant'Anna, Osvaldo Augusto; Tambourgi, Denise V.
Título: Quality of horse F(ab')2 antitoxins and antirabies immunoglobulins: protein content and anticomplementary activity
Fonte: J. venom. anim. toxins incl. trop. dis;24:16, 2018. tab, ilus.
Idioma: en.
Projeto: FAPESP.
Resumo: Among other applications, immunotherapy is used for the post-exposure treatment and/or prophylaxis of important infectious diseases, such as botulism, diphtheria, tetanus and rabies. The effectiveness of serum therapy is widely proven, but improvements on the immunoglobulin purification process and on the quality control are necessary to reduce the amount of protein aggregates. These may trigger adverse reactions in patients by activating the complement system and inducing the generation of anaphylatoxins. Herein, we used immunochemical methods to predict the quality of horse F(ab′)2 anti-botulinum AB, anti-diphtheric, antitetanic and anti-rabies immunoglobulins, in terms of amount of proteins and protein aggregates. Methods Samples were submitted to protein quantification, SDS-PAGE, Western blot analysis and molecular exclusion chromatography. The anticomplementary activity was determined in vitro by detecting the production of C5a/C5a desArg, the most potent anaphylatoxin. Data were analyzed by one-way ANOVA followed by Tukey's post-test, and differences were considered statistically significant when p < 0.05. Results Horse F(ab′)2 antitoxins and anti-rabies immunoglobulin preparations presented different amounts of protein. SDS-PAGE and Western blot analyses revealed the presence of protein aggregates, non-immunoglobulin contaminants and, unexpectedly, IgG whole molecules in the samples, indicating the non-complete digestion of immunoglobulins. The chromatographic profiles of antitoxins and anti-rabies immunoglobulins allowed to estimate the percentage of contaminants and aggregates in the samples. Although protein aggregates were present, the samples were not able to induce the generation of C5a/C5a desArg in vitro, indicating that they probably contain acceptable levels of aggregates. Conclusions Anti-botulinum AB (bivalent), anti-diphtheric, antitetanic and anti-rabies horse F(ab′)2 immunoglobulins probably contain acceptable levels of aggregates, although other improvements on the preparations must be carried out. Protein profile analysis and in vitro anticomplementary activity of F(ab′)2 immunoglobulin preparations should be included as quality control steps, to ensure acceptable levels of aggregates, contaminants and whole IgG molecules on final products, reducing the chances of adverse reactions in patients.(AU)
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: lil-603022
Autor: de Almeida C, Roberto Ronald.
Título: Leche de camello: características y perspectivas para uso en clínica / Camel milk: characteristics and perspectives for use in clinical practice
Fonte: Rev. chil. nutr;38(2):211-218, jun. 2011. tab.
Idioma: es.
Resumo: Camels are good milk producers and the selected animals are as good as the top cows for milk production. The camel milk has features that make it not only good as a supplement in the diet of humans, but in certain conditions such as allergies (does not contain beta-lactoglobulin, the cow's milk most important allergenic protein), lactose intolerance, general infections, diabetes, and even could be considered useful in the diet of patients with autism. Its use is, unfortunately, restricted to some populations where this animal is native. By its chemical composition does not clot in an acidic environment; it is rich in insulin, contains small dimeric immunoglobulins which suggest its use in molecular engineering. In conclusion, for its usefulness, camel farming should be encouraged, since these animals may become endangered animals.

Los camellos son buenos productores de leche y los animales seleccionados para esta finalidad, la producen en cantidad y calidad excelentes. La leche tiene características que hace bien no sólo como complemento alimenticio en la dieta de los seres humanos, sino también en ciertas condiciones, tales como alergias (no contiene beta-lactoglobulina, la proteína más alergénica de la leche de vaca), intolerancia a la lactosa, infecciones en general, diabetes y incluso podría considerarse útil en la alimentación de personas con autismo. Su uso está restringido a algunas poblaciones donde este animal es nativo. Por su composición química no se coagula en un medio ácido. Contiene gran cantidad de insulina y sus proteínas especiales sugieren el uso en la ingeniería molecular. En conclusión, la crianza de camellos debería estimularse, dado que estos animales están en peligro de extinción.
Responsável: CL334.1 - Biblioteca UBO


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Id: biblio-965302
Autor: Negri Filho, Luiz Carlos; Pereira, Célio Eduardo Sargentin; Chineze, Pedro Herique Newbery; Bogado, Alexey Leon Gomel; Bronkhorst, Dalton Evert; Lunardi, Michele; Okano, Werner.
Título: Use of the enzyme gamma-glutamyl transferase (GGT) as an indirect measure of passive transfer of immunity in holstein calves and association with the occurrence of diarrhea after birth / Uso da enzima gama-glutamiltransferase (GGT) como medida indireta de transferência de imunidade passiva em bezerras da raça holandesa e associação com a ocorrência de diarreia após o nascimento
Fonte: Biosci. j. (Online);32(2):455-459, mar./abr. 2016. tab, ilus.
Idioma: en.
Resumo: The objective of this study was to determine the gamma-glutamyl transferase (GGT) levels and its relationship with diarrhea and passive transfer of immunity in Holstein calves within 24 hours and 30 days of life from Leopolis municipality, the north Parana region. Colostrum is rich in immunoglobulins and vital for immunity to newborn calves, since bovine placenta does not allow the passage of immunoglobulin to the fetus. Calves undergo various challenges that can lead to disease and death in the first month of life, including diarrhea. Diarrhea has a multifactorial etiology, and the passive immunity transferred through ingestion of colostrum is able to protect the calf against many of these etiologic agents. GGT measurements indirectly infer the amount of immunoglobulin ingested by the calf. Higher serum GGT levels (381.72 IU / L) were found at 24 hours, and a significant reduction was observed at 30 days (66.22 IU / L). When the presence or absence of diarrhea was associated with GGT levels above and below 200 IU / L, no statistical significance (P> 0.05) was observed, since 80% of animals with diarrhea had serum GGT levels higher than 200 IU / L. Under the conditions of this study, there was no relationship between the GGT concentration and the occurrence of diarrhea, and no mortality was observed despite some animals presented diarrhea.

O colostro é rico em imunoglobulinas e vital para a imunidade de bezerros recém-nascidos, uma vez que placenta bovina não permite a passagem de imunoglobulinas para o feto. Os bezerros são submetidos a diversos desafios que podem levar à doença e morte no primeiro mês de vida, dentre as quais a diarreia. A etiologia da diarreia é multifatorial e a imunidade passiva transferida através da ingestão de colostro é capaz de proteger o bezerro contra muitos desses agentes etiológicos. O objetivo deste estudo foi determinar a dosagem de gama-glutamil transferase (GGT) e sua relação com diarreia e a transferência de imunidade passiva em novilhas da raça Holandesa nos tempos de 24 horas e 30 dias de vida, no município de Leópolis, na região norte do Paraná. As mensurações de GGT infere indiretamente a quantidade de imunoglobulinas ingeridas pelo bezerro. Os níveis séricos de GGT demonstraram valores médios maiores 24 horas após o nascimento (381,72 UI / L) e uma redução significativa foi observada aos 30 dias de vida (66,22 UI/L). Quando a presença ou ausência de diarreia foi associada com níveis de GGT acima e abaixo de 200UI/L, não foi observado significância estatística (p>0,05), uma vez que 80% dos animais com diarreia tinham níveis séricos de GGT superior a 200 UI/L. Sob as condições que foi realizado o presente estudo, não houve relação entre a concentração de GGT e a ocorrência de diarréia, e nenhuma mortalidade foi observada apesar de alguns animais apresentarem diarreia.
Responsável: BR396.1 - Biblioteca Central


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Id: lil-737135
Autor: Salazar Torres, Lay; Ávila Gamboa, Dalton.
Título: Inmunología perinatal / Perinatal immunology
Fonte: Femina;42(4):185-192, jul-ago. 2014.
Idioma: pt.
Resumo: Se ha considerado que el útero gestante es un lugar inmunológicamente privilegiado, donde el feto es protegido del rechazo por el sistema inmune materno, mediante un amplio repertorio de estrategias de evasión que contribuye a la sobrevivencia del feto. La gestación en sí misma constituye un acontecimiento de equilibrio inmunológico y la tolerancia inmunológica permite la progresión del embarazo, donde participan una secuencia sincronizada de eventos que se inicia desde la concepción y fertilización para dar lugar a la implantación y progresa hasta alcanzar un embarazo a término. El sistema inmune es la principal barrera que poseemos para protegernos de las infecciones. Durante la vida intrauterina, el feto está protegido por la madre de las agresiones externas, por lo que no necesita que su sistema inmunológico sea operativo, sin embargo, al nacer, recibe una avalancha de elementos extraños, por lo que necesitará disponer de cierta protección, así como una preparación para ejecutar las defensas necesarias para su protección inmunológica. La inmunidad sérica durante la vida fetal queda limitada a la transferencia a través de la placenta de IgG materna, a pesar de que el feto tiene la facultad de sintetizar inmunoglobulinas desde las primeras etapas de la gestación. Al nacimiento, el niño tiene su sistema inmunológico completo, aunque inmaduro, pero es capaz de responder a los estímulos antigénicos. Tiene múltiples anormalidades en el desarrollo de su sistema inmune, que involucran a los anticuerpos/inmunoglobulinas, complemento y granulocitos pudiendo contribuir a la alta incidencia de sus infecciones. El recién nacido carece de memoria inmunológica debido a que, en condiciones normales, el feto está exento de estímulos producidos por antígenos extraños. Dicha memoria se va adquiriendo a medida que entra en contacto con los diferentes antígenos. Se obtendrá cierta protección a las infecciones entéricas gracias a las IgA que aporta la lactancia materna. La exposición prenatal y postnatal a productos microbianos ambientales que pueden activar la inmunidad innata, puede acelerar el proceso de maduración del sistema inmune.(AU)

It has been considered the pregnant women`s womb as an immunological exceptional place, where fetus is protected against been rejected because of maternal immune system by means of a wide groups of evasive strategies that help in its survival. Pregnancy itself is an immunological equilibrium state and the immunological tolerance allow the progression of this event, where participate a synchronized sequence of biological events started from conception and fertilization to allow the implantation, and progress until to reach the pregnancy end. The immune system is our main barrier against infections. During intrauterine life fetus is protected by the mother against external aggressions, therefore he don`t need an operative immune system, nevertheless, at birth the new organisms receive an avalanche of strange elements needing some kind of protection as well as a preparation to carry out the necessary defense for his immunological protection. Serum immunity during fetal life is limited to the transference of maternal IgG through placenta, despite fetus capability to synthesize immunoglobulins from first stages of gestation. At birth the babe has a complete immunological system although immature but capable to respond to antigenic stimulus. He has multiples abnormalities in the immune system development that take account antibodies/immunoglobulin, complement and granulocytes contributing to his high incidence of infections. Newborn lack immunological memory because in normal conditions fetus is not stimulated by odd antigens. This memory is acquired through the contact with different antigens. It will be obtained some protection against enteric infections because IgA from maternal lactation. The prenatal and postnatal exposition to environmental microbial products that activate the innate immunity can accelerate the immune system maturing process.(AU)
Responsável: BR1365.1 - Biblioteca Biomédica A - CB/A


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Id: biblio-996644
Autor: Gomes, Carlos Henrique Rodrigues.
Título: Desenvolvimento de novos vetores para a produção de bibliotecas de anticorpos pelo sistema do phage display / Development of a antibody display library system targeted against vascular growth factor.
Fonte: São Paulo; s.n; 2018. 71 p. graf, tab.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Instituto de Química para obtenção do grau de Doutor.
Resumo: Anticorpos são moléculas de grande interesse científico e farmacêutico, principalmente, devido a sua alta especificidade contra antígenos determinados. Atualmente, anticorpos monoclonais estão entre os medicamentos (biofármacos) mais vendidos do mundo. São utilizados para o tratamento das mais diversas doenças, como câncer, retinopatias, doenças inflamatórias e do sistema imune, entre outras. Nos últimos 30 anos, as tecnologias para a obtenção de anticorpos monoclonais evoluíram muito, desde a tecnologia do hibridoma, até os processos de humanização de anticorpos murinos. Entre os métodos mais utilizados para a produção de anticorpos humanos, destaca-se a tecnologia do Phage Display. Nesta técnica, os genes que codificam as regiões variáveis de imunoglobulinas são inseridos no genoma de um bacteriófago, resultando na produção de partículas virais híbridas que contém fragmentos de anticorpos em fusão com uma das proteínas do capsídeo viral. Neste trabalho, desenvolvemos novos vetores para a apresentação de fragmentos ScFv em fusão com duas proteínas das proteínas do capsídeo viral, a pIII e pVIII. Os oligonucleotídeos utilizados para amplificar os genes de imunoglobulinas foram redesenhados e para minimizar a perda do repertório durante a produção da biblioteca, avaliamos em bancos de dados enzimas de restrição que não apresentam sítios de restrição nas sequencias gênicas. Esses sítios de restrição foram utilizados para construir as regiões de clonagem do vetor Phagemid. Outra etapa crítica na produção de bibliotecas de anticorpos é a reação do PCR de overlap, que pode restringir a diversidade de anticorpos e resultar na produção de amplicons codificando anticorpos truncados. Por isso, nossos vetores foram desenhados para permitir a clonagem direta das regiões variáveis das imunoglobulinas humanas ou murinas, sem a necessidade do PCR de overlap. Nossa expectativa, é que estes novos reagentes serão mais efetivos para a produção de novas bibliotecas de anticorpos pelo sistema do Phage Display

Antibodies are molecules of great scientific and pharmaceutical interest, mainly because of their high specificity against certain antigens. Currently, monoclonal antibodies are among the best selling drugs (biopharmaceuticals) in the world. They are used for the treatment of the most diverse disorders, such as cancer, retinopathies, inflammatory and immune system diseases, among others. In the past 30 years, technologies for obtaining monoclonal antibodies has greatly evolved from hybridoma technology to the humanization processes of murine antibodies. Among the methods used for the production of human antibodies, the technology of Phage Display stands out. In this technique, the genes encoding the immunoglobulin variable regions are inserted into the genome of a bacteriophage, resulting in the production of hybrid virus particles which contain fragments of antibodies in fusion with one of the viral capsid proteins. In this work, we developed new vectors for the presentation of ScFv fragments in fusion with two proteins of viral capsid proteins, pIII and pVIII. The oligonucleotides used to amplify the immunoglobulin genes were redesigned and to minimize repertory loss during library production, we evaluated restriction enzymes in databases that lack restriction sites in the gene sequences. These restriction sites were used to construct the cloning regions of the Phagemid vector. Another critical step in the production of antibody libraries is the overlap PCR reaction, which may restrict the diversity of antibodies and result in the production of amplicons encoding truncated antibodies. Therefore, our vectors were designed to allow the direct cloning of human or murine Immunoglobulins variable regions without the need for overlap PCR. Our expectation is that these new reagents will be more effective for the production of new antibody libraries by the Phage Display system
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas
BR40.1; T574.88, G633d. 30100026204-Q


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Id: lil-633024
Autor: Blanco, Alicia Noemí; Peirano, Andrea; Silvia, Grosso; Lazzari, María Angela.
Título: Efecto del inhibidor lúpico o anti-factor VIII sobre la actividad del factor VIII-sustrato cromogénico / Lupus anticoagulant or anti-factor VIII effect on factor VIII activity-chromogenic substrate
Fonte: Acta bioquím. clín. latinoam;41(3):399-405, jul.-sep. 2007. graf, tab.
Idioma: es.
Resumo: El objetivo del trabajo fue analizar el efecto de inmunoglobulinas (IgG) purificadas con actividad anti-factor VIII neutralizante o inhibidor lúpico, sobre el factor VIII medido por sustrato cromogénico (factor VIII SC), con el propósito de validar el método para detectar anti-factor VIII en presencia de inhibidor lúpico. Se aisló la fracción IgG (cromatografía de afinidad) a partir de plasmas normales (IgG-N), con anti-factor VIII (IgG-aFVIII) o con inhibidor lúpico (IgG-IL), preparándose un pool normal como aporte de factor VIII en los ensayos de inhibición. Se analizaron las mezclas del pool normal con IgG-N, IgG-aFVIII, IgG-IL o la combinación de IgG-aFVIII+IgG-IL, inmediatamente y luego de 2 h a 37 ºC, determinándose la actividad del factor VIII SC y el índice de inhibición inmediato (Ii) o post-incubación (Ip). La inhibición y la potenciación producida por IgG-aFVIII+IgG-IL (Ii:30%±0,7; Ip:55%±2,7), fueron similares a las observadas con IgG-aFVIII sola (Ii:30%±0,6; Ip:55%±2,7). La IgG-IL no afectó la actividad de factor VIII SC. Semejante a lo observado en plasma, la IgG-IL no interfirió en la inhibición del factor VIII SC mediada por IgG-aFVIII. Se confirmó así la especificidad del método amidolítico, que permite detectar inhibidores anti-factor VIII independientemente de la coexistencia con el inhibidor lúpico, solucionando el problema diagnóstico planteado en hemofilia A.

To analyse the effect of purified immunoglobulins (IgG) with anti-factor VIII or lupus anticoagulant activity on factor VIII measured by chromogenic substrate (factor VIIICS), in order to validate the chromogenic substrate method for detection of anti-factor VIII activity in the presence of lupus anticoagulant. IgG fractions were purified (affinity chromatography) from normal plasmas (IgG-N) and plasmas with anti-factor VIII (IgG-aFVIII) or lupus anticoagulant (IgG-LA). A normal plasma pool was prepared as source of factor VIII in the inhibition tests. Mixtures of this pool with IgG-N, IgG-aFVIII, IgG-LA or IgG-aFVIII+IgG-LA were tested immediately or after 2 h at 37 °C by factor VIIICS method; inhibition index (Ii) and post-incubation index (Ip) were calculated. The inhibitory and progressive inhibitory effects produced by IgG-aFVIII+IgG-LA (Ii:30%±0.7; Ip:55%±2.7) were similar to those observed with IgG-aFVIII (Ii:30%±0.6; Ip:55%±2.7). The IgG-LA did not inhibit the factor VIIIcs activity. As was observed in plasma samples, IgG-aFVIII and the mixture of IgG-aFVIII+IgG-LA inhibited factor VIIICS, whereas IgG-LA did not. These results confirm the specificity of the amidolytic method in detecting anti-factor VIII inhibitors independently of the presence of lupus anticoagulant, thus solving the diagnostic problem in haemophilia A.
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco



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