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Id: lil-719504
Autor: Miranda-Choque, Edwin; Candela-Herrera, Jorge; Díaz-Pereda, Javier; Fernández-Vega, Ana; Kolevic-Roca, Lenka; Patiño-Gabriel, Lilian; Chávez-Vereau, Pierre; Díaz-Noche, Maribel.
Título: Bartonella henselae en niños con adenitis regional atendidos en un hospital nacional del Perú, 2012 / Bartonella henselae in children with regional adenitis treated in a Peruvian national hospital, 2012
Fonte: Rev. peru. med. exp. salud publica;31(2):274-277, abr.-jun. 2014. tab.
Idioma: es.
Resumo: Con el objetivo de determinar la frecuencia de casos seropositivos a Bartonella henselae en niños con adenitis regional atendidos en un hospital nacional del Perú, se realizó un estudio trasversal en 106 niños con adenitis regional mayor de 1 cm de diámetro, de aparición aguda, con tiempo de enfermedad mayor de cinco días, atendidos en el Instituto Nacional de Salud del Niño durante el año 2012. Se definió seropositividad para B. henselae mediante el examen de inmunofluorescencia indirecta, siendo positivos 86 niños (81,1%) con una mediana de edad de 7 años, rango de 5 a 11; en el análisis bivariado se encontraron como factores asociados, edad mayor de 5 años, antecedentes de fiebre, adenopatía mayor de 4 cm y reporte de contacto con gato. En conclusión, los niños con adenitis regional atendidos en este hospital de referencia nacional presentaron una frecuencia alta de serología positiva para B. henselae.

In order to determine the frequency of seropositive cases of Bartonella henselae in children with regional adenitis treated in a national hospital in Peru, a cross-sectional study was conducted in 106 children with regional adenitis greater than 1 cm in diameter. The sample was selected from patients aged 5-11 years seen at the National Institute of Child Health for acute onset of regional adentitis, with more than five days of symptoms. B. henselae seropositivity was defined by indirect immunofluorescence test. We found that 86 children (81.1%) were positive for B.henselae. The median age of the patients was 7 years. In the bivariate analysis, the following associated factors were found: aged 5 years, history of fever, lymphadenopathy greater than 4 cm and reported contact with cat. In conclusion, children with regional adenitis treated in this national referral hospital showed a high frequency of positive serology for B. henselae.
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Id: lil-719494
Autor: Alarcón-Villaverde, Jorge O; Romani-Romani, Franco; Tejada, Romina A; Wong-Chero, Paolo; Céspedes-Zambrano, Manuel.
Título: Seroprevalencia de leptospirosis y características asociadas en agricultores de arroz de una región tropical del Perú / Leptospirosis seroprevalence and associated features in rice farmers of tropical region of Peru
Fonte: Rev. peru. med. exp. salud publica;31(2):195-203, abr.-jun. 2014. ilus, tab.
Idioma: es.
Resumo: Objetivos . Estimar la seroprevalencia de leptospirosis en agricultores dedicados al cultivo de arroz del valle del Alto Mayo, región San Martín e identificar anticuerpos para los serovares circulantes y los factores asociados a la infección. Materiales y métodos. Se realizó un estudio transversal entre octubre y noviembre de 2010 en una muestra de 254 agricultores procedentes de las once comisiones de regantes del valle de Alto Mayo. Se recolectó una muestra de sangre y se aplicó un cuestionario que incluyó variables sociodemográficas, y características del hogar y del trabajo. Se determinó la presencia de anticuerpos contra leptospira usando la prueba de microaglutinación y ELISA IgM. Resultados. La seroprevalencia determinada por microaglutinación fue del 64,6% (IC 95%: 58,6-70,6). La prueba ELISA IgM fue reactiva en 15,0% (IC 95%: 10,5-19,5). Los tipos de serovar más frecuentes fueron Icterohaemorrhagiae, Autumnalis, Australis , Panama y Grippotyphosa. Los factores asociados independientemente fueron la manipulación de roedores (OR ajustado [ORa] de 7,9, IC 95%: 1,6-37,9), trabajar descalzo (ORa de 2,9, IC 95%: 1,2-6,8) y el sexo masculino (ORa de 4,5, IC 95%: 1,3-15,3). Conclusiones . El valle del Alto Mayo es un área hiperendémica de leptospirosis. Los factores asociados más importantes fueron la práctica de trabajar descalzo y la de manipular roedores en los campos de cultivo. En consecuencia, se recomienda implementar intervenciones que incidan en estos dos factores a fin de reducir el riesgo de infección en los cultivadores de arroz.

Objectives. To estimate the seroprevalence of leptospirosis in rice farmers of the Alto Mayo Valley in the Peruvian region of San Martín and to identify factors associated with infection. Materials and methods. A cross-sectional study was conducted between October and November of 2010 on a sample of 254 farmers from eleven irrigation committees in the Alto Mayo Valley. A blood sample was collected as well as sociodemographic, household and labor characteristics in the form of a questionnaire. The presence of antibodies against leptospira was determined using the microscopic agglutination test and the IgM ELISA. Results. The seroprevalence by agglutination was 64.6% (95% CI: 58.6-70.6). The IgM ELISA test was reactive in 15,0% (95% CI: 10.5-19.5). The most frequent serovar were Icterohaemorrhagiae, Autumnalis, Australis, Panama and Grippotyphosa. Independently associated factors were handling rodents (adjusted OR (aOR): 7.9; 95% CI: 1.6-37.9), working barefoot (aOR: 2.9; 95% CI: 1.2-6.8) and male sex (aOR: 4.5; 95% CI: 1.3-15.3). Conclusions. The Alto Mayo Valley is a hyperendemic for leptospirosis. The most important factors were associated with working barefoot and handling rodents in crop fields. Consequently, it is recommended to implement interventions that affect these two factors in order to reduce the risk of infection in rice farmers.
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Id: lil-763282
Autor: Soares, Catharina de Paula Oliveira Cavalcanti; Teles, José Andreey Almeida; Santos, Aldenir Feitosa dos; Silva, Stemberg Oliveira Firmino; Cruz, Maria Vilma Rocha Andrade; Silva-Júnior, Francisco Feliciano da.
Título: Prevalence of Brucella spp in humans / Prevalência da Brucella spp em humanos / Prevalencia de la Brucella spp en humanos
Fonte: Rev. latinoam. enferm;23(5):919-926, Sept.-Oct. 2015. tab, graf.
Idioma: en.
Resumo: Objective: to determine the seroprevalence of Brucella spp in humans.Method: this is an observational study, developed with 455 individuals between 18 and 64 years old, who use the Estratégia de Saúde da Família (Brazil's family health strategy). The serum samples of volunteers underwent buffered acid antigen tests, such as screening, agar gel immunodiffusion and slow seroagglutination test in tubes and 2-Mercaptoethanol.Results: among the samples, 1.98% has responded to buffered-acid antigen, 2.85% to agar gel immunodiffusion test and 1.54% to the slow seroagglutination tests on tubes/2-Mercaptoethanol. The prevalence of Brucella spp was 4.4%, represented by the last two tests.Conclusion: the results of this research suggest that the studied population is exposed to Brucella spp infection.

Objetivo: determinar a soroprevalência da Brucella spp em humanos.Método: trata-se de estudo observacional, desenvolvido com 455 indivíduos entre 18 e 64 anos, selecionados, que utilizavam a estratégia de saúde da família. As amostras de soro dos voluntários foram submetidas aos testes de antígeno acidificado tamponado, como triagem, imunodifusão em gel de ágar e aos testes de soroaglutinação lenta em tubos e 2-mercaptoetanol.Resultados: dentre as amostras, 1,98% reagiram ao antígeno acidificado tamponado, 2,85% à imunodifusão em gel ágar e 1,54% aos testes de soroaglutinação lenta em tubos/2-mercaptoetanol. Sendo a prevalência da Brucella spp de 4,4%, representada pelos dois últimos testes.Conclusão: os resultados desta pesquisa sugerem que a população estudada encontra-se exposta à infecção por Brucella spp.

Objetivo: determinar la seroprevalencia de la Brucella spp en humanos.Método: se trata de un estudio observacional, desarrollado con 455 individuos seleccionados con edades entre 18 y 64 años, que utilizaban la Estrategia de Salud de la Familia. Las muestras de suero de los voluntarios fueron sometidas a las pruebas de antígeno acidificado tamponado, como tamizaje, inmunodifusión en gel de agar y a las pruebas de seroaglutinación lenta en tubos y 2-mercaptoetanol.Resultados: entre las muestras; el 1,98% reaccionó al antígeno acidificado tamponado, el 2,85% reaccionó a la inmunodifusión en gel agar; y el 1,54%, a las pruebas de seroaglutinación lenta en tubos/2-mercaptoetanol. La prevalencia de laBrucella spp representada por las dos últimas pruebas fue del 4,4%.Conclusión: los resultados de esta investigación sugieren que la población que ha sido estudiada se encuentra expuesta a la infección por Brucellaspp.
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Id: biblio-889239
Autor: Wang, Jiashun; Li, Yi; Chen, Jia; Hua, Deping; Li, Yi; Deng, Hui; Li, Ying; Liang, Zhixuan; Huang, Jinhai.
Título: Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene
Fonte: Braz. j. microbiol;49(2):320-328, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Tianjin Science and Technology.
Resumo: Abstract Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. The results showed that the PagC polyclonal antiserum is of good specificity and the capture rate of 0.1 mg IMBs for Salmonella tended to be stable at the range of 70-74% corresponding to the concentrations between 101 and 104 CFU/mL. The method developed demonstrated high specificity for the positive Salmonella samples when compared to non-specific DNA samples, such as Escherichia coli, Staphylococcus aureus, Yersinia enterocolitica, and Yersinia pseudotuberculosis. The limit of detection of this assay was 18 CFU/mL. Detection and quantitative enumeration of Salmonella in samples of pork or milk shows good recoveries of 54.34% and 52.07%. In conclusion, the polyclonal antibody of recombinant PagC protein is effective to capture Salmonella from detected samples. The developed pagC antibody IMBs-qPCR method showed efficiency, sensitivity and specificity for 30 Salmonella detection, enabling detection within 10 h, which is a promising rapid method to detect Salmonella in emergency.
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Id: biblio-889161
Autor: Mirhosseini, Seyed Ali; Fooladi, Abbas Ali Imani; Amani, Jafar; Sedighian, Hamid.
Título: Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis
Fonte: Braz. j. microbiol;48(4):774-781, Oct.-Dec. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen. The immunization was conducted in mice with the purified recombinant FliC (rFliC). The mice were subcutaneously immunized with rFliC and elicited significant rFliC specific serum IgG antibodies. An indirect ELISA system was established for the detection of Salmonella Enteritidis. Our results confirmed that the recombinant flagellin can be one of the excellent indicators for the detection of Salmonella Enteritidis.
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Id: biblio-889131
Autor: Oliveira, Marluce Aparecida Assunção; Leal, Élida Aparecida; Correia, Max Assunção; Serufo Filho, José Carlos; Dias, Ricardo Souza; Serufo, José Carlos.
Título: Human leptospirosis: occurrence of serovars of Leptospira spp. in the state of Minas Gerais, Brazil, from 2008 to 2012
Fonte: Braz. j. microbiol;48(3):483-488, July-Sept. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Background: Leptospirosis is an infectious and acute disease caused by Leptospira spp. that have high epidemic potential. This study verified the main Leptospira spp. serovars detected by MAT from serum of patients with suspicion of leptospirosis from 2008 to 2012 in Minas Gerais State. Methods: The laboratory received sera from 4654 patients. All serum were screened by IgM-ELISA according to the manufacturer's instructions. Each sample reactive or indeterminate were tested against twenty-four serovars of Leptospira by MAT. Results: In this study, 597 patients were classified as reactive on MAT. Only 301 patients were confirmed by laboratory test. It was not possible confirmation by laboratory diagnosis of 296 patients. Among the samples classified as reactive on MAT, 273 patients exhibited titers bigger than 800 for one or more serovars; seroconversion was detected in 28 cases. Percentage of 85.1% of the samples reactive on MAT corresponded to males, 39.4% corresponded to patients aged between 20 and 39 years old. The most common serovars found were Icterohaemorrhagiae, Andamana, Patoc, Tarassovi, Copenhageni, Hardjo and Australis. Concerning the samples that exhibited titers bigger than 800, serovar Icterohaemorrhagiae was also the most common, followed by Copenhageni, Andamana, Patoc, Tarassovi, Grippotyphosa and Canicola. In this study, 40% of the cases occurred to the metropolitan area, state capital and 34 neighboring towns. Conclusion: Our results show the possibly spreading serovars in Minas Gerais State and contribute to knowledge of human leptospirosis, aiming at improving the prevention, control of the disease, as well as the treatment of infected patients.
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Id: biblio-889077
Autor: dos Santos, DP; Muniz, IPR; Queiroz, AF; Pereira, IS; Souza, MPA; Lima, LJ; Sousa, LRO; Ribeiro, IS; Galantini, MPL; Marques, LM; Figueiredo, TB; da Silva, RAA.
Título: Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;51(5):e6773, 2018. graf.
Idioma: en.
Resumo: Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted protection in C57BL/6 mice by reducing the bacterial, which was correlated with increased serum concentration of IgG antibodies (IgG1 and IgG2a) against S. aureus. The increase in IgG2a antibody levels was correlated with a decrease of bacterial load in intradermally immunized C57BL/6 mice, along with production of IL-17A at the inflammation site, as well as IgG1consumption. Similar results were not found in the A/j lineage. In conclusion, a vaccine against S. aureus should focus more on the individual characteristics of the host because it is a determinant factor for the success of the immunization.
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Id: biblio-842001
Autor: Ricardo, Paloma; Oliveira, Luís G; Baraldi, Thaís G; Mechler, Marina L; Almeida, Henrique M. S; Silva, Glaucenyra C. P. da; Gatto, Igor R. H; Mathias, Luis A.
Título: Occurrence of anti-Brucella antibodies in intensive pig farming and in non-technified pig herds / Ocorrência de anticorpos anti-Brucella em suínos de granjas comercias e de criações de subsistência
Fonte: Pesqui. vet. bras = Braz. j. vet. res;36(10):930-934, out. 2016. tab, mapas.
Idioma: en.
Resumo: The aim of this study was to determine the occurrence of antibodies anti-Brucella in 1,940 swine blood samples. Out of the 1,940 sera samples, 1,594 were from 30 intensive pig farming from seven different states, collected during the slaughtering of animals, and 346 samples from 56 non-technified (subsistence) pig herds from Jaboticabal region, São Paulo State, Brazil. All samples were tested by Buffered Plate Acidified Antigen (BPAA) and considered positive in case of agglutination. If positive, the samples were tested by Complement Fixation Reaction (CFR) as a confirmatory test. Out of the 1,594, two were positive for BPAA but negative for CFR, so the occurrence was 0%. Among the 346 samples, two were positive for BPAA but only one was positive in the confirmatory test, whith a titer of 1:8. Thus, the occurrence was 0.29%, an important result to demonstrate the improvement of the sanitary status of Brazilian non-technified pig herds, despite the low conditions of production.(AU)

O presente estudo foi realizado com o objetivo de se determinar a ocorrência de anticorpos anti-Brucella spp. em 1.940 amostras de sangue de suínos, das quais 1.594 amostras eram de 30 granjas comerciais, de sete diferentes Estados, coletadas durante o abate dos animais, e 346 amostras de 56 criações de subsistência da região de Jaboticabal, Estado de São Paulo, Brasil. Todas as amostras foram submetidas ao teste do Antígeno Acidificado Tamponado (AAT) e consideradas positivas caso ocorresse aglutinação. Quando positivas, as amostras eram submetidas ao teste de Reação de Fixação de Complemento (RFC) como teste confirmatório. Dentre as 1.594 amostras de suínos de granjas comerciais, duas se mostraram sensíveis ao AAT, porém, quando foram submetidas à RFC, ambas apresentaram reação negativa, levando a uma porcentagem de ocorrência de 0%. Já entre as 346 amostras de criações de subsistência, duas foram positivas ao AAT, sendo que apenas uma apresentou reação positiva no teste confirmatório, cujo título foi de 1:8. Desta forma, a ocorrência foi de 0,29%, resultado importante para demonstrar a melhoria do status sanitário dos rebanhos de subsistência brasileiros, apesar das condições precárias em que vivem.(AU)
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Id: biblio-840027
Autor: Nadaf, Maria Isabel Valdomir; Lima, Laila; Stranieri, Inês; Takano, Olga Akiko; Carneiro-Sampaio, Magda; Palmeira, Patricia.
Título: Passive acquisition of anti-Staphylococcus aureus antibodies by newborns via transplacental transfer and breastfeeding, regardless of maternal colonization
Fonte: Clinics;71(12):687-694, Dec. 2016. tab, graf.
Idioma: en.
Projeto: AUX-PE-DINTER/NF.
Resumo: OBJECTIVE: To investigate the transmission of anti-Staphylococcus aureus (Sa) IgG, IgG1 and IgG2 via placental transfer and the transfer of IgA via the colostrum according to maternal Sa carrier status at delivery. METHODS: We evaluated anti-Sa IgG, IgG1 and IgG2 in maternal and cord sera and IgA in colostrum from a case (n=49, Sa+) and a control group (n=98, Sa-). RESULTS: Of the 250 parturients analyzed for this study, 49 were nasally colonized with S. aureus (prevalence of 19.6%). Ninety-eight non-colonized subjects were selected for the control group. The anti-Sa IgG, IgG1 and IgG2 levels and the IgG avidity indexes in the maternal and cord sera did not differ between the groups, with a low transfer ratio of anti-Sa IgG to the newborns in both groups. The anti-Sa IgG2 titers were significantly higher than the IgG1 titers in the maternal and cord sera. Inversely, the transfer ratios were higher for anti-Sa IgG1 compared with IgG2; however, no differences between the groups were detected. The Sa-specific IgA levels and avidity indexes in the colostrum were equivalent between groups. CONCLUSIONS: Maternal Sa nasal colonization at delivery is not associated with higher antibody levels in the mother or newborns. The high titers of anti-Sa IgG2 found in the cord serum indicate a greater reactivity with non-protein antigens, which may further contribute to the susceptibility to staphylococcal infections at birth. The presence of IgA in the colostrum with avidity to S. aureus reinforces the importance of breastfeeding shortly after birth.
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Id: biblio-839291
Autor: Gioia, CAC; Lemos, APS; Gorla, MCO; Mendoza-Sassi, R; Figueredo, BS; Ballester, T; Von Groll, A; Wedig, B; Ethur, NV; Bragança, L; Silva, PEA; Milagres, LG.
Título: Seroprevalence of bactericidal antibodies against serogroup B and C Meningococci in a University Hospital
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(5):e5590, 2017. tab, graf.
Idioma: en.
Resumo: Meningococcus serogroup B (MenB), clonal complex 32 (cc 32), was the Brazilian epidemic strain of meningococcal disease (MD) in the 1990’s. Currently, meningococcus serogroup C (MenC), cc 103, is responsible for most of the cases of the disease in Brazil. The aim of this study was to investigate the seroprevalence of bactericidal antibody (SBA) against representative epidemic strains of MenC, (N753/00 strain, C:23:P1.22,14-6, cc103) and MenB, (Cu385/83 strain, B:4,7:P1.15,19, cc32) in students and employees of a university hospital in the State of Rio Grande do Sul (RS, Brazil). A second MenC strain (N79/96, C:2b:P1.5-2,10, cc 8) was used as a prototype strain of Rio de Janeiro’s outbreak that occurred in the 1990’s. Our previous study showed a 9% rate of asymptomatic carriers in these same individuals. A second goal was to compare the SBA prevalence in meningococcal carriers and non-carriers. Fifty-nine percent of the studied population showed protective levels of SBA titers (log2≥2) against at least one of the three strains. About 40% of the individuals had protective levels of SBA against N753/00 and Cu385/83 strains. Nonetheless, only 22% of the individuals showed protective levels against N79/96 strain. Significantly higher antibody levels were seen in carriers compared to non-carriers (P≤0.009). This study showed that, similar to other States in Brazil, a MenC (23:P1.22,14-6, cc103) strain with epidemic potential is circulating in this hospital. Close control by the Epidemiological Surveillance Agency of RS of the number of cases of MD caused by MenC strains in the State is recommended to prevent a new disease outbreak.
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