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  1 / 1517 MEDLINE  
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PMID:29408624
Autor:Wang X; Li W; Xu X; Wang W; He K; Fan H
Dirección:Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; College of Veterinary Medicine, Nanjing
Título:Phylogenetic analysis of two goat-origin PCV2 isolates in China.
Fuente:Gene; 651:57-61, 2018 Apr 20.
ISSN:1879-0038
País de publicación:Netherlands
Idioma:eng
Resumen:Complete genome characterization of non-porcine origin Porcine circovirus type 2 (PCV2) was first described in 2014 in China. In the present study, we first identified PCV2 nucleotides in goat samples and the prevalence of PCV2 in goat was 6.15%. However, only two new strains, Goat2014-4 and Goat2014-5, could be completely sequenced. The genome of the strain Goat2014-4, which collected from the goat infected with PPRV, contains 1766 nt; strain Goat2014-5, which originated from a healthy goat, is comprised of 1767 nt. The results showed that they shared the highest nucleotide identity with BDH and the lowest similarity with DK1980PMWSfree strain and they belonged only to genotype PCV2d. Meanwhile, they shared higher homology with porcine-origin PCV2 strains than others. Moreover, a detailed analysis of the capsid amino acid sequences revealed that there were distinct differences for goat2014-4 (708 bp) and goat2014-5 (705 bp); strain Goat2014-4 showed an elongation of two amino acids, and strains Goat2014-5 showed an elongation of one amino acid compared with other reference strains. This is the first report of the genetic analysis of goat-origin PCV2 isolates. It also provides an additional supported evidence for cross-species transmission of PCV2.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Capsid Proteins)


  2 / 1517 MEDLINE  
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PMID:29192047
Autor:Collins PJ; McKillen J; Allan G
Dirección:Veterinary Sciences Division, Agri-Food and Biosciences Institute, Belfast, Northern Ireland BT4 3SD.
Título:Porcine circovirus type 3 in the UK.
Fuente:Vet Rec; 181(22):599, 2017 12.
ISSN:2042-7670
País de publicación:England
Idioma:eng
Tipo de publicación:LETTER


  3 / 1517 MEDLINE  
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PMID:29374509
Autor:Jin J; Park C; Cho SH; Chung J
Dirección:Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul 00380, Republic of Korea; Department of Biomedical Science, Seoul National University College of Medicine, Seoul 00380, Republic of Korea.
Título:The level of decoy epitope in PCV2 vaccine affects the neutralizing activity of sera in the immunized animals.
Fuente:Biochem Biophys Res Commun; 496(3):846-851, 2018 02 12.
ISSN:1090-2104
País de publicación:United States
Idioma:eng
Resumen:Viral pathogens have evolved a wide range of tactics to evade host immune responses and thus propagate effectively. One efficient tactic is to divert host immune responses toward an immunodominant decoy epitope and to induce non-neutralizing antibodies toward this epitope. Therefore, it is expected that the amount of decoy epitope in a subunit vaccine can affect the level of neutralizing antibody in an immunized animal. In this study, we tested this hypothesis by generating an antibody specific to the decoy epitope on the capsid protein of porcine circovirus type 2 (PCV2). Using this antibody, we found that two commercial vaccines contained statistically different amounts of the decoy epitope. The vaccine with lower levels of decoy epitope induced a significantly higher level of neutralizing antibody after immunization. This antibody can be used as an analytical tool to monitor the quality of a vaccine from batch to batch.
Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nombre de substancia:0 (Adenovirus Vaccines); 0 (Antibodies, Neutralizing); 0 (Epitopes); 0 (Viral Vaccines)


  4 / 1517 MEDLINE  
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PMID:29246996
Autor:Li X; Tian K
Dirección:3 Cuiwei Road, High-tech District, Luoyang, China 471003.
Título:Porcine circovirus type 3: a threat to the pig industry?
Fuente:Vet Rec; 181(24):659-660, 2017 12 16.
ISSN:2042-7670
País de publicación:England
Idioma:eng
Tipo de publicación:LETTER; COMMENT


  5 / 1517 MEDLINE  
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PMID:29079448
Autor:Wang ZJ; Xu CM; Song ZB; Wang M; Liu QY; Jiang P; Li YF; Bai J; Wang XW
Dirección:Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Título:Vimentin modulates infectious porcine circovirus type 2 in PK-15 cells.
Fuente:Virus Res; 243:110-118, 2018 01 02.
ISSN:1872-7492
País de publicación:Netherlands
Idioma:eng
Resumen:Porcine circovirus type 2 (PCV2) is the pathogen that causes postweaning multisystemic wasting syndrome, which leads to significant economic losses for swine farms worldwide. However, the infection mechanism of PCV2 is not completely understood yet. Vimentin is a part of the cytoskeleton network and plays an important role in several virus infections. It is not clear whether vimentin has a role in PCV2 infection nor how it affects PCV2 infection. In this study, the function of vimentin in PK-15 cells infected with PCV2 has been elucidated. We found that vimentin had a restrictive effect on the replication of PCV2 in PK-15 cells. Overexpression of vimentin by transferred pCAGGS-vimentin and down-regulation by the respective scrambled small interfering RNA showed that vimentin restricted the replication and virion production of PCV2. A special interaction between vimentin and PCV2 Cap protein was observed using laser confocal microscopy and immunoprecipitation assay. Moreover, overexpression of vimentin could decrease NF-κB activity and increase PCV2-induced caspase-3 activity in PK-15 cells. These data suggest that vimentin is involved in the replication of PCV2 and has a restrictive effect on it, which is helpful in the study of the replication mechanism of PCV2.
Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nombre de substancia:0 (NF-kappa B); 0 (Vimentin)


  6 / 1517 MEDLINE  
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PMID:29031627
Autor:Chen GH; Tang XY; Sun Y; Zhou L; Li D; Bai Y; Mai KJ; Li YY; Wu QW; Ma JY
Dirección:College of Animal Science, South China Agricultural University, Guangzhou, China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong, China.
Título:Development of a SYBR green-based real-time quantitative PCR assay to detect PCV3 in pigs.
Fuente:J Virol Methods; 251:129-132, 2018 Jan.
ISSN:1879-0984
País de publicación:Netherlands
Idioma:eng
Resumen:Porcine circovirus 3 (PCV3) has been reported in cases of porcine dermatitis and nephropathy syndrome, reproductive failure, cardiac and multi-systemic inflammation. A SYBR green-based real-time quantitative PCR (qPCR) assay was established in this study to detect PCV3 in 203 clinical samples from suckling piglets affected by congenital tremors in China. The limit of detection (LOD) of PCV3 was 1.73×10 copies/µL for gel electrophoresis and 1.73×10 copies/µL for SYBR green-based real-time qPCR. The melt curve analysis showed a single melt peak at 82.5°C.The intra-assay coefficient of variation was up to 1.83% and the inter-assay coefficient of variation was up to 2.27%. The PCV3 positive detection rate of 203 clinical samples for the SYBR green-based real-time qPCR and the conventional PCR was 86.70% (176/203) and 26.60% (54/203), respectively. Each tissue detected in the SYBR green-based real-time qPCR showed a higher positive rate than that detected in the conventional PCR. These results indicated that the SYBR green-based real-time qPCR assay is a powerful method with high sensitivity, specificity and reproducibility for epidemiological investigations of PCV3.
Tipo de publicación:EVALUATION STUDIES; JOURNAL ARTICLE
Nombre de substancia:0 (Organic Chemicals); 163795-75-3 (SYBR Green I)


  7 / 1517 MEDLINE  
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PMID:28463055
Autor:Stenzel T; Koncicki A
Dirección:a Department of Poultry Diseases, Faculty of Veterinary Medicine , University of Warmia and Mazury , Olsztyn , Poland.
Título:The epidemiology, molecular characterization and clinical pathology of circovirus infections in pigeons - current knowledge.
Fuente:Vet Q; 37(1):166-174, 2017 Dec.
ISSN:1875-5941
País de publicación:Netherlands
Idioma:eng
Resumen:The first cases of circovirus infections in pigeons were documented less than 25 years ago. Since then, circovirus infections have been reported on nearly all continents. The specificity of pigeon breeding defies biosecurity principles, which could be the reason for the high prevalence of PiCV infections. PiCV infections in pigeons lead to atrophy of immune system organs and lymphocyte apoptosis. Infected birds could be more susceptible to infections of the respiratory and digestive tract. PiCV has been associated with the young pigeon disease syndrome (YPDS). PiCVs are characterized by high levels of genetic diversity due to frequent point mutations, recombination processes in the PiCV genome and positive selection. Genetic recombinations and positive selection play the key role in the evolution of PiCV. A protocol for culturing PiCV under laboratory conditions has not yet been developed, and traditional vaccines against the infection are not available. Recombinant capsid proteins for detecting anti-PiCV antibodies have been obtained, and these antigens can be used in the production of diagnostic tests and subunit vaccines against PiCV infections. However, YPDS has complex etiology, and it remains unknown whether immunization against PiCV alone will contribute to effective control of YPDS.
Tipo de publicación:JOURNAL ARTICLE; REVIEW


  8 / 1517 MEDLINE  
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PMID:29073194
Autor:Yang N; Qiao J; Liu S; Zou Z; Zhu L; Liu X; Zhou S; Li H
Dirección:College of Animal Science and Technology, Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, P. R., China.
Título:Change in the immune function of porcine iliac artery endothelial cells infected with porcine circovirus type 2 and its inhibition on monocyte derived dendritic cells maturation.
Fuente:PLoS One; 12(10):e0186775, 2017.
ISSN:1932-6203
País de publicación:United States
Idioma:eng
Resumen:Porcine circovirus-associated disease is caused by porcine circovirus type 2 (PCV2) infection, which targets iliac artery endothelial cells (PIECs); it leads to severe immunopathologies and is associated with major economic losses in the porcine industry. Here, we report that in vitro PCV2 infection of PIECs causes cell injury, which affects DC function as well as adaptive immunity. Specifically, PCV2 infection downregulated PIEC antigen-presenting molecule expression, upregulated cytokines involved in the immune and inflammatory response causing cell damage and repair, and altered the migratory capacity of PIECs. In addition, PCV2-infected PIECs inhibited DC maturation, enhanced the endocytic ability of DCs, and weakened the stimulatory effect of DCs on T lymphocytes. Together, these findings indicate that profound functional impairment of DCs in the presence of PCV2-infected PIECs may be a potential pathogenic mechanism associated with PCV2-induced porcine disease.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Cytokines)


  9 / 1517 MEDLINE  
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PMID:28922413
Autor:Fraiberk M; Hájková M; Krulová M; Kojzarová M; Drda Morávková A; Psikal I; Forstová J
Dirección:Charles University, Faculty of Science, Prague, Czech Republic.
Título:Exploitation of stable nanostructures based on the mouse polyomavirus for development of a recombinant vaccine against porcine circovirus 2.
Fuente:PLoS One; 12(9):e0184870, 2017.
ISSN:1932-6203
País de publicación:United States
Idioma:eng
Resumen:The aim of this study was to develop a suitable vaccine antigen against porcine circovirus 2 (PCV2), the causative agent of post-weaning multi-systemic wasting syndrome, which causes significant economic losses in swine breeding. Chimeric antigens containing PCV2b Cap protein sequences based on the mouse polyomavirus (MPyV) nanostructures were developed. First, universal vectors for baculovirus-directed production of chimeric MPyV VLPs or pentamers of the major capsid protein, VP1, were designed for their exploitation as vaccines against other pathogens. Various strategies were employed based on: A) exposure of selected immunogenic epitopes on the surface of MPyV VLPs by insertion into a surface loop of the VP1 protein, B) insertion of foreign protein molecules inside the VLPs, or C) fusion of a foreign protein or its part with the C-terminus of VP1 protein, to form giant pentamers of a chimeric protein. We evaluated these strategies by developing a recombinant vaccine against porcine circovirus 2. All candidate vaccines induced the production of antibodies against the capsid protein of porcine circovirus after immunization of mice. The candidate vaccine, Var C, based on fusion of mouse polyomavirus and porcine circovirus capsid proteins, could induce the production of antibodies with the highest PCV2 neutralizing capacity. Its ability to induce the production of neutralization antibodies was verified after immunization of pigs. The advantage of this vaccine, apart from its efficient production in insect cells and easy purification, is that it represents a DIVA (differentiating infected from vaccinated animals) vaccine, which also induces an immune response against the mouse polyoma VP1 protein and is thus able to distinguish between vaccinated and naturally infected animals.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Capsid Proteins); 0 (Recombinant Fusion Proteins); 0 (VP1 protein, Poliovirus); 0 (Viral Vaccines)


  10 / 1517 MEDLINE  
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PMID:28885140
Autor:Regnard GL; de Moor WRJ; Hitzeroth II; Williamson AL; Rybicki EP
Dirección:1​Biopharming Research Unit, Department of Molecular and Cell Biology, Faculty of Science, University of Cape Town, Rondebosch 7701, Cape Town, South Africa.
Título:Xenogenic rolling-circle replication of a synthetic beak and feather disease virus genomic clone in 293TT mammalian cells and Nicotiana benthamiana.
Fuente:J Gen Virol; 98(9):2329-2338, 2017 Sep.
ISSN:1465-2099
País de publicación:England
Idioma:eng
Resumen:The preparation of infectious beak and feather disease circovirus virions (BFDV) has until now relied on the extraction of virus from whole tissue of deceased or euthanized parrots known to be infected with the virus. Extraction from diseased tissue is necessary, as the virus has yet to be grown in vitro using tissue-cultured cells from any source. While infectious DNA clones have been synthesized for porcine and duck circoviruses, and both replicate in host cells and result in active viral infection in animals, this has not been shown for BFDV. The aim of this study was to prepare an infectious BFDV genomic clone that could be used as challenge material in birds for vaccine testing. A putatively infectious BFDV genomic clone was designed and tested in mammalian cell culture, and in the plant Nicotiana benthamiana in the presence of plant-specific ssDNA geminivirus replication components. Replication was assessed using rolling-circle amplification, qPCR, replication-deficient clones and rescue plasmids. We showed that a synthetic partially dimeric BFDV genomic clone self-replicated when transfected into 293TT mammalian cells, and was also replicated in N. benthamiana in the presence of geminivirus replication elements. This is the first report of a BFDV genome replicating in any cell system, and the first report of a circovirus replicating with the aid of a geminivirus in a plant. Both of these developments could open up possibilities for making reagents and vaccines for BFDV, testing vaccine efficacy and investigating viral replication using rationally designed artificial genomes.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (DNA, Viral)



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