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  1 / 251 MEDLINE  
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PMID:28715975
Autor:Shulman LM; Davidson I
Dirección:Department of Epidemiology and Preventive Medicine, School of Public Health, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel; email: lester.shulman@bezeqint.net.
Título:Viruses with Circular Single-Stranded DNA Genomes Are Everywhere!
Fuente:Annu Rev Virol; 4(1):159-180, 2017 Sep 29.
ISSN:2327-0578
País de publicación:United States
Idioma:eng
Resumen:Circular single-stranded DNA viruses infect archaea, bacteria, and eukaryotic organisms. The relatively recent emergence of single-stranded DNA viruses, such as chicken anemia virus (CAV) and porcine circovirus 2 (PCV2), as serious pathogens of eukaryotes is due more to growing awareness than to the appearance of new pathogens or alteration of existing pathogens. In the case of the ubiquitous human circular single-stranded DNA virus family Anelloviridae, there is still no convincing direct causal relation to any specific disease. However, infections may play a role in autoimmunity by changing the homeostatic balance of proinflammatory cytokines and the human immune system, indirectly affecting the severity of diseases caused by other pathogens. Infections with CAV (family Anelloviridae, genus Gyrovirus) and PCV2 (family Circoviridae, genus Circovirus) are presented here because they are immunosuppressive and affect health in domesticated animals. CAV shares genomic organization, genomic orientation, and common features of major proteins with human anelloviruses, and PCV2 DNA may be present in human food and vaccines.
Tipo de publicación:JOURNAL ARTICLE; REVIEW
Nombre de substancia:0 (DNA, Circular); 0 (DNA, Single-Stranded); 0 (DNA, Viral)


  2 / 251 MEDLINE  
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PMID:28326326
Autor:Fang L; Li Y; Wang Y; Fu J; Cui S; Li X; Chang S; Zhao P
Dirección:College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, China.
Título:Genetic Analysis of Two Chicken Infectious Anemia Virus Variants-Related in Stray Mice and Dogs: The First Report in China, 2015.
Fuente:Biomed Res Int; 2017:6707868, 2017.
ISSN:2314-6141
País de publicación:United States
Idioma:eng
Resumen:Chicken infectious anemia virus (CIAV) causes acute viral infection in chickens worldwide. It can infect chickens of all ages, but the disease is seen only in young chickens and is characterized by hemorrhagic lesions in the muscles, atrophic changes in the lymphoid organs, aplastic bone marrow, and immunosuppression causing increased mortality. Previous studies have demonstrated that CIAV can be isolated from blood specimens of humans and fecal samples of stray cats. In the present study, two variants of CIAV were isolated from fecal samples of mice (CIAV-Mouse) and stray dogs (CIAV-Dog), respectively. The genome of the two CIAV variants was sequenced and the results of the recombination detection program suggested that the CIAV-Dog strain could be a recombinant viral strain generated from parental CIAV strains, AB119448 and GD-1-12, with high confidence. Particularly, these findings were obtained from the comparison of genetic diversity and the relationship of CIAV between different hosts. This is the first report indicating that there is a significant difference in the number of transcription factor binding sites in CIAV noncoding regions from different hosts. Further studies are required to investigate the large geographic distribution of CIAV and monitor the variants, host range, and associated diseases.
Tipo de publicación:JOURNAL ARTICLE


  3 / 251 MEDLINE  
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PMID:28034642
Autor:Ruiz-Martínez S; Pantoja-Uceda D; Castro J; Vilanova M; Ribó M; Bruix M; Benito A; Laurents DV
Dirección:Laboratori d'Enginyeria de Proteïnes, Departament de Biologia, Facultat de Ciències, Universitat de Girona, and Institut d'Investigació Biomedica de Girona Josep Trueta (IdIBGi), c/ Maria Aurèlia Campmany 40, 17003, Girona, Spain.
Título:Insights into the mechanism of Apoptin's exquisitely selective anti-tumor action from atomic level characterization of its conformation and dynamics.
Fuente:Arch Biochem Biophys; 614:53-64, 2017 Jan 15.
ISSN:1096-0384
País de publicación:United States
Idioma:eng
Resumen:Apoptin is a 121 residue protein which forms large, soluble aggregates and possesses an exceptionally selectively cytotoxic action on cancer cells. In the accompanying paper, we described the design, production and initial characterization of an Apoptin truncated variant called H -ApopΔProΔLeu. Whereas both the variant and wild type protein possess similar selective cytotoxicity against cancer cells following transfection, only the variant is cytotoxic when added externally. Remarkably, as observed by gel filtration chromatography and dynamic light scattering, H -ApopΔProΔLeu lacks the tendency of wild type Apoptin to form large aggregates, which greatly facilitated the study of its biological properties. Here, we characterize the conformation and dynamics of H -ApopΔProΔLeu. Using a battery of 2D, 3D and (4,2)D NMR spectra, the essentially complete H, C and N resonance assignments of H -ApopΔProΔLeu were obtained. The analysis of these data shows that the variant is an intrinsically disordered protein, which lacks a preferred conformation. This conclusion is corroborated by a lack of protection against proteolytic cleavage and hydrogen/deuterium exchange. Moreover, the CD spectra are dominated by random coil contributions. Finally, H- N NOE ratios are low, which indicates flexibility on the ps-ns time scale. Interestingly, H -ApopΔProΔLeu's intrinsically disordered ensemble is not significantly altered by the redox state of its Cys residues or by Thr phosphorylation, which has been proposed to play a key role in Apoptin's selective cytotoxicity. These results serve to better comprehend Apoptin's remarkably selective anticancer action and provide a framework for the future design of improved Apoptin variants.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Antineoplastic Agents); 0 (Capsid Proteins); 0 (VP3 protein, Chicken anemia virus); EC 3.4.21.64 (Endopeptidase K); K848JZ4886 (Cysteine)


  4 / 251 MEDLINE  
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PMID:27933433
Autor:Kaffashi A; Eshratabadi F; Shoushtari A
Dirección:Razi Vaccine and Serum Research Institute, Hesarak, Karaj, Iran. amkaffashi@yahoo.com.
Título:Full-length infectious clone of an Iranian isolate of chicken anemia virus.
Fuente:Virus Genes; 53(2):312-316, 2017 Apr.
ISSN:1572-994X
País de publicación:United States
Idioma:eng
Resumen:An Iranian field strain of chicken anemia virus (CAV), designated IR CAV, was isolated in the Marek's disease virus-transformed lymphoblastoid cell line MDCC-MSB1 (MSB1) culture for the first time. The full-length CAV DNA of this strain was cloned in the bacterial plasmid pTZ57R/T to create the molecular clone pTZ-CAV. The nucleotide and deduced amino acid sequences of viral proteins of IR CAV were compared with those of representative CAV sequences including reference and commercial vaccine strains. IR CAV was not related to vaccine strains and also found to have glutamine at positions 139 and 144 confirming previous studies in which such mutations were associated with a slow rate of virus spread in cell culture. pTZ-CAV was digested with PstI to release IR CAV DNA and then transfected into MSB1 cell by electroporation. The transfected cells showed cytopathic effect similar to virion-initiated infection. One-day old specific pathogen-free chicks were inoculated with the regenerated virus, which had been obtained from transfected MSB1 cells, and compared with the chicks inoculated with IR CAV. Gross lesions in the birds inoculated with the regenerated virus illustrated the infectious nature of the regenerated virus from the cloned IR CAV DNA.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (DNA, Viral); 0 (Viral Proteins)


  5 / 251 MEDLINE  
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PMID:27899306
Autor:Ganar K; Shah M; Kamdi BP; Kurkure NV; Kumar S
Dirección:Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
Título:Molecular characterization of chicken anemia virus outbreaks in Nagpur province, India from 2012 to 2015.
Fuente:Microb Pathog; 102:113-119, 2017 Jan.
ISSN:1096-1208
País de publicación:England
Idioma:eng
Resumen:Chicken anemia virus (CAV) is one of the important poultry pathogen. CAV infection can cause immunosuppression, aggravation of co-infections, vaccination failures and mortality. We are reporting the CAV outbreaks from the Nagpur province of India, between the years 2012-2015. The breeds included cockerel and Black Australorp of age varying from 29 to 50 days. The mortality rate observed among poultry was from 20% to 62.5%. Clinical symptoms like anemia, subcutaneous hemorrhages, growth retardation, abnormal feathers and hind limb paralysis suggested CAV infection. Postmortem analysis showed hemorrhages in thigh muscle and atrophy of the thymus and bone marrow. Seven out of 11 samples showed positive amplification of the CAV genome upon PCR. Phylogenetic analysis of all the seven isolates based on VP1 gene nucleotide sequence suggested circulation of genotype A strains in Maharashtra. The study will help us understand the circulating genotype of CAV in India and formulate its diagnosis and vaccination.
Tipo de publicación:JOURNAL ARTICLE


  6 / 251 MEDLINE  
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PMID:27624642
Autor:Pohjola L; Tammiranta N; Ek-Kommonen C; Soveri T; Hänninen ML; Fredriksson Ahomaa M; Huovilainen A
Dirección:a Department of Production Animal Medicine, Faculty of Veterinary Medicine , University of Helsinki , Saarentaus , Finland.
Título:A survey for selected avian viral pathogens in backyard chicken farms in Finland.
Fuente:Avian Pathol; 46(2):166-172, 2017 Apr.
ISSN:1465-3338
País de publicación:England
Idioma:eng
Resumen:Backyard poultry are regaining popularity in Europe and increased interest in the health and management of non-commercial farms has resulted. Furthermore, commercial poultry farm owners have become concerned about the risk represented by contagious avian diseases that nearby backyard poultry could transmit. Fifty-one voluntary backyard chicken farms were visited between October 2012 and January 2013. Blood samples and individual cloacal swabs were collected from 457 chickens. In 44 farms (86%), one or more of the tested chickens had antibodies against avian encephalomyelitis and chicken infectious anaemia viruses, 24 farms (47%) had chickens seropositive for infectious bronchitis virus, 10 farms (20%) had chickens seropositive for infectious bursal disease virus, six farms (12%) had chickens seropositive for infectious laryngotracheitis virus and two farms (5.4%) had chickens seropositive for avian influenza virus. No farms had chickens seropositive for Newcastle disease virus. Of the 51 farms, five (10%) had chickens positive for coronavirus reverse transcription polymerase chain reaction. A phylogenetic analysis showed that all backyard chicken coronaviruses collected were QX type infectious bronchitis viruses. All chickens tested for avian influenza and Newcastle disease viruses using real time reverse transcription polymerase chain reaction were negative. To our knowledge, there is no evidence to date to suggest that these diseases would have been transmitted between commercial and non-commercial flocks.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Antibodies, Viral)


  7 / 251 MEDLINE  
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PMID:27512067
Autor:Kucharski TJ; Ng TF; Sharon DM; Navid-Azarbaijani P; Tavassoli M; Teodoro JG
Dirección:Goodman Cancer Research Centre, McGill University, Montréal, QC, Canada Department of Biochemistry, McGill University, Montréal, QC, Canada.
Título:Activation of the Chicken Anemia Virus Apoptin Protein by Chk1/2 Phosphorylation Is Required for Apoptotic Activity and Efficient Viral Replication.
Fuente:J Virol; 90(20):9433-45, 2016 Oct 15.
ISSN:1098-5514
País de publicación:United States
Idioma:eng
Resumen:UNLABELLED: Chicken anemia virus (CAV) is a single-stranded circular DNA virus that carries 3 genes, the most studied of which is the gene encoding VP3, also known as apoptin. This protein has been demonstrated to specifically kill transformed cells while leaving normal cells unharmed in a manner that is independent of p53 status. Although the mechanistic basis for this differential activity is unclear, it is evident that the subcellular localization of the protein is important for the difference. In normal cells, apoptin exists in filamentous networks in the cytoplasm, whereas in transformed cells, apoptin is present in the nucleus and appears as distinct foci. We have previously demonstrated that DNA damage signaling through the ataxia telangiectasia mutated (ATM) pathway induces the translocation of apoptin from the cytoplasm to the nucleus, where it induces apoptosis. We found that apoptin contains four checkpoint kinase consensus sites and that mutation of either threonine 56 or 61 to alanine restricts apoptin to the cytoplasm. Furthermore, treatment of tumor cells expressing apoptin with inhibitors of checkpoint kinase 1 (Chk1) and Chk2 causes apoptin to localize to the cytoplasm. Importantly, silencing of Chk2 rescues cancer cells from the cytotoxic effects of apoptin. Finally, treatment of virus-producing cells with Chk inhibitor protects them from virus-mediated toxicity and reduces the titer of progeny virus. Taken together, our results indicate that apoptin is a sensor of DNA damage signaling through the ATM-Chk2 pathway, which induces it to migrate to the nucleus during viral replication. IMPORTANCE: The chicken anemia virus (CAV) protein apoptin is known to induce tumor cell-specific death when expressed. Therefore, understanding its regulation and mechanism of action could provide new insights into tumor cell biology. We have determined that checkpoint kinase 1 and 2 signaling is important for apoptin regulation and is a likely feature of both tumor cells and host cells producing virus progeny. Inhibition of checkpoint signaling prevents apoptin toxicity in tumor cells and attenuates CAV replication, suggesting it may be a future target for antiviral therapy.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Capsid Proteins); 0 (VP3 protein, Chicken anemia virus); EC 2.7.1.11 (Checkpoint Kinase 2); EC 2.7.11.1 (CHEK1 protein, human); EC 2.7.11.1 (CHEK2 protein, human); EC 2.7.11.1 (Checkpoint Kinase 1)


  8 / 251 MEDLINE  
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PMID:27405124
Autor:Tang MB; Chang HM; Wu WC; Chou YC; Yu CP
Título:FIRST DETECTION OF CHICKEN ANEMIA VIRUS AND NOROVIRUS GENOGROUP II IN STOOL OF CHILDREN WITH ACUTE GASTROENTERITIS IN TAIWAN.
Fuente:Southeast Asian J Trop Med Public Health; 47(3):416-23, 2016 May.
ISSN:0125-1562
País de publicación:Thailand
Idioma:eng
Resumen:To date, there has been no report of co-infection of chicken anemia virus (CAV) with enteric virus in patients with acute gastroenteritis (AGE). CAV has been recently detected in various types of human samples including stool, indicating pathogenicity in gastrointestinal tract. Examination by PCR-based methods of CAV and norovivus genogroup II (NV GII) in stool of 110 children with AGE at a hospital in Taiwan revealed for the first time of co-infection in two cases. This is the first description of CAV infection in children with AGE in Taiwan. Systematic surveillance and evidence-based studies are required to determine the transmission pathways and spread of CAV in Taiwan.
Tipo de publicación:JOURNAL ARTICLE


  9 / 251 MEDLINE  
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PMID:27396163
Autor:Fang L; Li X; Ren Z; Li Y; Wang Y; Cui Z; Chang S; Zhao P
Título:[Effect of Low Dose of Chicken Infectious Anemia Virus in Attenuated Vaccine on SPF Chicken Body Weight and Vaccine Immune Antibody].
Fuente:Bing Du Xue Bao; 32(2):190-4, 2016 Mar.
ISSN:1000-8721
País de publicación:China
Idioma:chi
Resumen:In order to observe the effect of the immune and weight of chickens after use the attenuated vaccine with low dose of chicken infectious anemia virus (CIAV). In this study, the effects of low dose of CIAV on the weight of SPF chickens and NDV antibody production were observed by simulated experiments. The results showed that 10 EID50 and 5 EID50 CIAV per plume attenuated NDV vaccines were used to cause the weight loss of SPF chickens. Compared with the use of the non contaminated vaccine group, it has significant difference. And NDV antibody levels compared with the use of the non contaminated groups also decreased after use the vaccine with two doses of CIAV contaminated. It has significant difference. A certain proportion of CIAV antibody positive was detected at the beginning of the second week after use the NDV vaccine with two doses of CIAV contaminated. The detection of a high proportion of CIAV nucleic acid was detected in the first week after the use of a contaminated vaccine. The results of the study demonstrate the effects of CIAV pollution on the production and immune function of SPF chickens, and it is suggested that increasing the detection of viral nucleic acid can help save time and improve the detection rate in the detection of exogenous virus contamination by SPF chicken test method.
Tipo de publicación:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nombre de substancia:0 (Antibodies, Viral); 0 (Vaccines, Attenuated)


  10 / 251 MEDLINE  
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PMID:27298822
Autor:Li Y; Wang Y; Fang L; Fu J; Cui S; Zhao Y; Cui Z; Chang S; Zhao P
Dirección:College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
Título:Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China.
Fuente:Biomed Res Int; 2016:4275718, 2016.
ISSN:2314-6141
País de publicación:United States
Idioma:eng
Resumen:The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV. In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoculating into MSB1 cells and PCR amplification. A CIAV strain (SD1403) was isolated and successfully identified. Three overlapping genomic fragments were obtained by PCR amplification and sequencing. The full genome sequence of the SD1403 strain was obtained by aligning the sequences. The genome of the SD1403 strain was 2293 bp with a nucleotide identity of 94.8% to 98.5% when compared with 30 referred CIAV strains. The viral proteins VP2 and VP3 were highly conserved, but VP1 was not relatively conserved. Both amino acids 139 and 144 of VP1 were glutamine, which was in accord with the low pathogenic characteristics. In this study, we first reported that CIAV exists in Chinese SPF chicken populations and may be an important reason why attenuated vaccine can be contaminated with CIAV.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Antibodies, Viral)



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