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  1 / 139 MEDLINE  
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PMID:27771067
Autor:Beato MS; Tassoni L; Milani A; Salviato A; Di Martino G; Mion M; Bonfanti L; Monne I; Watson SJ; Fusaro A
Dirección:Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, PD, Italy. Electronic address: msbeato@izsvenezie.it.
Título:Circulation of multiple genotypes of H1N2 viruses in a swine farm in Italy over a two-month period.
Fuente:Vet Microbiol; 195:25-29, 2016 Nov 15.
ISSN:1873-2542
País de publicación:Netherlands
Idioma:eng
Resumen:In August 2012 repeated respiratory outbreaks caused by swine influenza A virus (swIAV) were registered for a whole year in a breeding farm in northeast Italy that supplied piglets for fattening. The virus, initially characterized in the farm, was a reassortant Eurasian avian-like H1N1 (H1 N1) genotype, containing a haemagglutinin segment derived from the pandemic H1N1 (A(H1N1)pdm09) lineage. To control infection, a vaccination program using vaccines against the A(H1N1)pdm09, human-like H1N2 (H1 N2), human-like H3N2 (H3N2), and H1 N1 viruses was implemented in sows in November 2013. Vaccine efficacy was assessed by sampling nasal swabs for two months in 35-75 day-old piglets born from vaccinated sows. Complete genome sequencing of eight swIAV-positive nasal swabs collected longitudinally from piglets after the implementation of the vaccination program was conducted to investigate the virus characteristics. Over the two-month period, two different genotypes involving multiple reassortment events were detected. The unexpected circulation of multiple reassortant genotypes in such a short time highlights the complexity of the genetic diversity of swIAV and the need for a better surveillance plan, based on the combination of clinical signs, epidemiological data and whole genome characterization.
Tipo de publicación:JOURNAL ARTICLE


  2 / 139 MEDLINE  
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PMID:28494344
Autor:Xu G; Zhang X; Liu Q; Bing G; Hu Z; Sun H; Xiong X; Jiang M; He Q; Wang Y; Pu J; Guo X; Yang H; Liu J; Sun Y
Dirección:Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China; China Institute of Veterinary Drug Control, Beijing, China.
Título:PA-X protein contributes to virulence of triple-reassortant H1N2 influenza virus by suppressing early immune responses in swine.
Fuente:Virology; 508:45-53, 2017 Aug.
ISSN:1096-0341
País de publicación:United States
Idioma:eng
Resumen:Previous studies have identified a functional role of PA-X for influenza viruses in mice and avian species; however, its role in swine remains unknown. Toward this, we constructed PA-X deficient virus (Sw-FS) in the background of a Triple-reassortment (TR) H1N2 swine influenza virus (SIV) to assess the impact of PA-X in viral virulence in pigs. Expression of PA-X in TR H1N2 SIV enhanced viral replication and host protein synthesis shutoff, and inhibited the mRNA levels of type I IFNs and proinflammatory cytokines in porcine cells. A delay of proinflammatory responses was observed in lungs of pigs infected by wild type SIV (Sw-WT) compared to Sw-FS. Furthermore, Sw-WT virus replicated and transmitted more efficiently than Sw-FS in pigs. These results highlight the importance of PA-X in the moderation of virulence and immune responses of TR SIV in swine, which indicated that PA-X is a pro-virulence factor in TR SIV in pigs.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (PA-X protein, influenza A virus); 0 (Repressor Proteins); 0 (Viral Nonstructural Proteins); 9008-11-1 (Interferons)


  3 / 139 MEDLINE  
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PMID:28222327
Autor:Chen LJ; Lin XD; Tian JH; Liao Y; Ying XH; Shao JW; Yu B; Guo JJ; Wang MR; Peng Y; Shi M; Holmes EC; Yang ZQ; Zhang YZ
Dirección:State Key Laboratory of Virology/Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China; State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Ins
Título:Diversity, evolution and population dynamics of avian influenza viruses circulating in the live poultry markets in China.
Fuente:Virology; 505:33-41, 2017 May.
ISSN:1096-0341
País de publicación:United States
Idioma:eng
Resumen:Live poultry markets (LPMs) are an important source of novel avian influenza viruses (AIV). During 2015-2016 we surveyed AIV diversity in ten LPMs in Hubei, Zhejiang and Jiangxi provinces, China. A high diversity and prevalence of AIVs (totaling 12 subtypes) was observed in LPMs in these provinces. Strikingly, however, the subtypes discovered during 2015-2016 were markedly different to those reported by us in these same localities one year previously, suggesting a dynamic shift in viral genetic diversity over the course of a single year. Phylogenetic analyses revealed frequent reassortment, including between high and low pathogenic AIV subtypes and among those that circulate in domestic and wild birds. Notably, the novel H5N6 reassortant virus, which contains a set of H9N2-like internal genes, was prevalent in all three regions surveyed. Overall, these data highlight the profound changes in genetic diversity and in patterns of reassortment in those AIVs that circulate in LPMs.
Tipo de publicación:JOURNAL ARTICLE


  4 / 139 MEDLINE  
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PMID:28207684
Autor:Blanton L; Mustaquim D; Alabi N; Kniss K; Kramer N; Budd A; Garg S; Cummings CN; Fry AM; Bresee J; Sessions W; Garten R; Xu X; Elal AI; Gubareva L; Barnes J; Wentworth DE; Burns E; Katz J; Jernigan D; Brammer L
Dirección:Influenza Division, National Center for Immunization and Respiratory Diseases, CDC.
Título:Update: Influenza Activity - United States, October 2, 2016-February 4, 2017.
Fuente:MMWR Morb Mortal Wkly Rep; 66(6):159-166, 2017 Feb 17.
ISSN:1545-861X
País de publicación:United States
Idioma:eng
Resumen:This report summarizes U.S. influenza activity* during October 2, 2016-February 4, 2017, and updates the previous summary (1). Influenza activity in the United States began to increase in mid-December, remained elevated through February 4, 2017, and is expected to continue for several more weeks. To date, influenza A (H3N2) viruses have predominated overall, but influenza A (H1N1)pdm09 and influenza B viruses have also been identified.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Antiviral Agents)


  5 / 139 MEDLINE  
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PMID:28131371
Autor:Cappuccio J; Dibarbora M; Lozada I; Quiroga A; Olivera V; Dángelo M; Pérez E; Barrales H; Perfumo C; Pereda A; Pérez DR
Dirección:Instituto de Virología, CICVyA, INTA, Nicolas Repetto y de los Reseros s/n, 1686, Hurlingham, Buenos Aires, Argentina; CONICET, Av. Rivadavia 1917, C1033AAJ, CABA, Argentina. Electronic address: cappuccio.javier@inta.gob.ar.
Título:Two years of surveillance of influenza a virus infection in a swine herd. Results of virological, serological and pathological studies.
Fuente:Comp Immunol Microbiol Infect Dis; 50:110-115, 2017 Feb.
ISSN:1878-1667
País de publicación:England
Idioma:eng
Resumen:Swine farms provide a dynamic environment for the evolution of influenza A viruses (IAVs). The present report shows the results of a surveillance effort of IAV infection in one commercial swine farm in Argentina. Two cross-sectional serological and virological studies (n=480) were carried out in 2011 and 2012. Virus shedding was detected in nasal samples from pigs from ages 7, 21 and 42-days old. More than 90% of sows and gilts but less than 40% of 21-days old piglets had antibodies against IAV. In addition, IAV was detected in 8/17 nasal swabs and 10/15 lung samples taken from necropsied pigs. A subset of these samples was further processed for virus isolation resulting in 6 viruses of the H1N2 subtype (δ2 cluster). Pathological studies revealed an association between suppurative bronchopneumonia and necrotizing bronchiolitis with IAV positive samples. Statistical analyses showed that the degree of lesions in bronchi, bronchiole, and alveoli was higher in lungs positive to IAV. The results of this study depict the relevance of continuing long-term active surveillance of IAV in swine populations to establish IAV evolution relevant to swine and humans.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Antibodies, Viral)


  6 / 139 MEDLINE  
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PMID:28033315
Autor:Shang M; Blanton L; Kniss K; Mustaquim D; Alabi N; Barnes S; Budd A; Davlin SL; Kramer N; Garg S; Cummings CN; Flannery B; Fry AM; Grohskopf LA; Olsen SJ; Bresee J; Sessions W; Garten R; Xu X; Elal AI; Gubareva L; Barnes J; Wentworth DE; Burns E; Katz J; Jernigan D; Brammer L
Título:Update: Influenza Activity - United States, October 2-December 17, 2016.
Fuente:MMWR Morb Mortal Wkly Rep; 65(5051):1439-1444, 2016 Dec 30.
ISSN:1545-861X
País de publicación:United States
Idioma:eng
Resumen:This report summarizes U.S. influenza activity during October 2-December 17, 2016. Influenza activity in the United States remained low in October and has been slowly increasing since November. Influenza A viruses were identified most frequently, with influenza A (H3N2) viruses predominating. Most influenza viruses characterized during this period were genetically or antigenically similar to the reference viruses representing vaccine components recommended for production in the 2016-17 Northern Hemisphere influenza vaccines.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Antiviral Agents)


  7 / 139 MEDLINE  
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PMID:27795418
Autor:Takemae N; Harada M; Nguyen PT; Nguyen T; Nguyen TN; To TL; Nguyen TD; Pham VP; Le VT; Do HT; Vo HV; Le QV; Tran TM; Nguyen TD; Thai PD; Nguyen DH; Le AQ; Nguyen DT; Uchida Y; Saito T
Dirección:Division of Transboundary Animal Disease, National Institute of Animal Health, NARO, Tsukuba, Japan.
Título:Influenza A Viruses of Swine (IAV-S) in Vietnam from 2010 to 2015: Multiple Introductions of A(H1N1)pdm09 Viruses into the Pig Population and Diversifying Genetic Constellations of Enzootic IAV-S.
Fuente:J Virol; 91(1), 2017 Jan 01.
ISSN:1098-5514
País de publicación:United States
Idioma:eng
Resumen:Active surveillance of influenza A viruses of swine (IAV-S) involving 262 farms and 10 slaughterhouses in seven provinces in northern and southern Vietnam from 2010 to 2015 yielded 388 isolates from 32 farms; these viruses were classified into H1N1, H1N2, and H3N2 subtypes. Whole-genome sequencing followed by phylogenetic analysis revealed that the isolates represented 15 genotypes, according to the genetic constellation of the eight segments. All of the H1N1 viruses were entirely A(H1N1)pdm09 viruses, whereas all of the H1N2 and H3N2 viruses were reassortants among 5 distinct ancestral viruses: H1 and H3 triple-reassortant (TR) IAV-S that originated from North American pre-2009 human seasonal H1, human seasonal H3N2, and A(H1N1)pdm09 viruses. Notably, 93% of the reassortant IAV-S retained M genes that were derived from A(H1N1)pdm09, suggesting some advantage in terms of their host adaptation. Bayesian Markov chain Monte Carlo analysis revealed that multiple introductions of A(H1N1)pdm09 and TR IAV-S into the Vietnamese pig population have driven the genetic diversity of currently circulating Vietnamese IAV-S. In addition, our results indicate that a reassortant IAV-S with human-like H3 and N2 genes and an A(H1N1)pdm09 origin M gene likely caused a human case in Ho Chi Minh City in 2010. Our current findings indicate that human-to-pig transmission as well as cocirculation of different IAV-S have contributed to diversifying the gene constellations of IAV-S in Vietnam. IMPORTANCE: This comprehensive genetic characterization of 388 influenza A viruses of swine (IAV-S) isolated through active surveillance of Vietnamese pig farms from 2010 through 2015 provides molecular epidemiological insight into the genetic diversification of IAV-S in Vietnam after the emergence of A(H1N1)pdm09 viruses. Multiple reassortments among A(H1N1)pdm09 viruses and enzootic IAV-S yielded 14 genotypes, 9 of which carried novel gene combinations. The reassortants that carried M genes derived from A(H1N1)pdm09 viruses became predominant, replacing those of the IAV-S that had been endemic in Vietnam since 2011. Notably, one of the novel reassortants likely caused a human case in Vietnam. Given that Vietnam is the second-largest pig-producing country in Asia, continued monitoring of IAV-S is highly important from the viewpoints of both the swine industry and human public health.
Tipo de publicación:JOURNAL ARTICLE


  8 / 139 MEDLINE  
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PMID:27766828
Título:Zoonotic influenza viruses: antigenic and genetic characteristics and development of candidate vaccine viruses for pandemic preparedness.
Título:Virus grippaux zoonotiques: caractéristiques génétiques et antigéniques et mise au point de virus vaccinaux candidats pour se préparer à une pandémie..
Fuente:Wkly Epidemiol Rec; 91(42):485-99, 2016 Oct 16.
ISSN:0049-8114
País de publicación:Switzerland
Idioma:eng; fre
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Influenza Vaccines)


  9 / 139 MEDLINE  
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PMID:27512056
Autor:Talker SC; Stadler M; Koinig HC; Mair KH; Rodríguez-Gómez IM; Graage R; Zell R; Dürrwald R; Starick E; Harder T; Weissenböck H; Lamp B; Hammer SE; Ladinig A; Saalmüller A; Gerner W
Dirección:Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
Título:Influenza A Virus Infection in Pigs Attracts Multifunctional and Cross-Reactive T Cells to the Lung.
Fuente:J Virol; 90(20):9364-82, 2016 Oct 15.
ISSN:1098-5514
País de publicación:United States
Idioma:eng
Resumen:UNLABELLED: Pigs are natural hosts for influenza A viruses and play a critical role in influenza epidemiology. However, little is known about their influenza-evoked T-cell response. We performed a thorough analysis of both the local and systemic T-cell response in influenza virus-infected pigs, addressing kinetics and phenotype as well as multifunctionality (gamma interferon [IFN-γ], tumor necrosis factor alpha [TNF-α], and interleukin-2 [IL-2]) and cross-reactivity. A total of 31 pigs were intratracheally infected with an H1N2 swine influenza A virus (FLUAVsw) and consecutively euthanized. Lungs, tracheobronchial lymph nodes, and blood were sampled during the first 15 days postinfection (p.i.) and at 6 weeks p.i. Ex vivo flow cytometry of lung lymphocytes revealed an increase in proliferating (Ki-67(+)) CD8(+) T cells with an early effector phenotype (perforin(+) CD27(+)) at day 6 p.i. Low frequencies of influenza virus-specific IFN-γ-producing CD4(+) and CD8(+) T cells could be detected in the lung as early as 4 days p.i. On consecutive days, influenza virus-specific CD4(+) and CD8(+) T cells produced mainly IFN-γ and/or TNF-α, reaching peak frequencies around day 9 p.i., which were up to 30-fold higher in the lung than in tracheobronchial lymph nodes or blood. At 6 weeks p.i., CD4(+) and CD8(+) memory T cells had accumulated in lung tissue. These cells showed diverse cytokine profiles and in vitro reactivity against heterologous influenza virus strains, all of which supports their potential to combat heterologous influenza virus infections in pigs. IMPORTANCE: Pigs not only are a suitable large-animal model for human influenza virus infection and vaccine development but also play a central role in the emergence of new pandemic strains. Although promising candidate universal vaccines are tested in pigs and local T cells are the major correlate of heterologous control, detailed and targeted analyses of T-cell responses at the site of infection are scarce. With the present study, we provide the first detailed characterization of magnitude, kinetics, and phenotype of specific T cells recruited to the lungs of influenza virus-infected pigs, and we could demonstrate multifunctionality, cross-reactivity, and memory formation of these cells. This, and ensuing work in the pig, will strengthen the position of this species as a large-animal model for human influenza virus infection and will immediately benefit vaccine development for improved control of influenza virus infections in pigs.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Influenza Vaccines); 0 (Interleukin-2); 0 (Tumor Necrosis Factor-alpha); 82115-62-6 (Interferon-gamma)


  10 / 139 MEDLINE  
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PMID:27230301
Autor:Liu H; Tao J; Zhang P; Yin X; Ha Z; Zhang C
Dirección:Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, 201106, Shanghai, China; Municipal Key Laboratory of Agri-Genetics and Breedings, Shanghai 201106, China. Electronic address: huilil@163.com.
Título:Pathogenic characteristics of a novel triple-reasserted H1N2 swine influenza virus.
Fuente:Biologicals; 44(4):252-6, 2016 Jul.
ISSN:1095-8320
País de publicación:England
Idioma:eng
Resumen:A novel triple reasserted H1N2 virus A/swine/Shanghai/1/2007 (SH07) was isolated from nasal swabs of weaned pig showing clinical symptoms of coughing and sneezing. To explore the virus characteristics, mice, chickens and pigs were selected for pathogenicity study. Pigs inoculated intranasally with 10(6) TCID50 SH07 showed clinical symptoms with coughing and sneezing, but no death. The virus nuclear acid was detected in many tissues using real-time PCR, which was mainly distributed in respiratory system particularly in the lungs. The virus was low-pathogenic to chickens with 10(6) TCID50 dose inoculation either via intramuscular or intranasal routes. However virus nuclear acid detection and virus isolation confirmed that the virus can also be found in nasal and rectum. When virus was inoculated into mice by intramuscular or intranasal routes we observed 100% and 80% lethality respectively. The third generation of samples passaged on MDCK cell were SIV positive in indirect immunofluorescence assay (IFA) using antiserum against H1N2 SIV. Furthermore, the lungs of mice showed obvious lesion with interstitial pneumonia. Data in our study suggest that SH07 is preferentially pathogenic to mammals rather than birds although it is a reasserting virus with the fragments from swine, human and avian origin.
Tipo de publicación:JOURNAL ARTICLE



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