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  1 / 9654 MEDLINE  
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PMID:29368481
Autor:Yermishin AP; Svitoch OV; Voronkova EV; Gukasian ON; Luksha VI
Título:[Determination of the composition and the allelic state of disease and pest resistance genes in potato parental lines using DNA markers].
Fuente:Genetika; 52(5):569-78, 2016 May.
ISSN:0016-6758
País de publicación:Russia (Federation)
Idioma:rus
Resumen:The allelic dosage of disease and pest resistance genes was determined in 11 prospective potato varieties and hybrids by means of detecting the corresponding PCR DNA markers in their progeny from crosses with specially selected testers. It was revealed that most (65%) resistance genes in the analyzed parental lines were present as a single dominant allele (simplex). Nevertheless, we were able to find some multiplex lines valuable for breeding. The Yanka variety and the clone 52-03-16 had one triplex and one duplex of resistance genes, the Lilea and Charaunik varieties and the clone 106-04-17 had two resistance genes in duplex, and the Uladar and Falvarak varieties and the clone 45-04-24 were duplex for some single genes. The highest number of multiplex lines was detected for the genes Ry sto , H1, and Sen1. Only simplex genotypes were revealed for the Gro-1-4 and PLRV1 genes.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Genetic Markers)


  2 / 9654 MEDLINE  
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PMID:29350615
Autor:Santos JC; Nascimento ALT
Dirección:Laboratório Especial de Desenvolvimento de Vacinas-Centro de Biotecnologia, Instituto Butantan, São Paulo, Brazil.
Título:Chimeras could help in the fight against leptospirosis.
Fuente:Elife; 7, 2018 01 19.
ISSN:2050-084X
País de publicación:England
Idioma:eng
Resumen:Understanding the structure of an antigen can guide the design of improved antigen-based vaccines.
Tipo de publicación:JOURNAL ARTICLE; COMMENT
Nombre de substancia:0 (Antibodies, Bacterial); 0 (Antigens, Bacterial)


  3 / 9654 MEDLINE  
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PMID:28743038
Autor:Durkovic J; Husárová H; Javoríková L; Canová I; Suleková M; Kardosová M; Lukácik I; Mamonová M; Lagana R
Dirección:Department of Phytology, Technical University, T.G. Masaryka 24, 960 53 Zvolen, Slovak Republic. Electronic address: jaroslav.durkovic@tuzvo.sk.
Título:Physiological, vascular and nanomechanical assessment of hybrid poplar leaf traits in micropropagated plants and plants propagated from root cuttings: A contribution to breeding programs.
Fuente:Plant Physiol Biochem; 118:449-459, 2017 Sep.
ISSN:1873-2690
País de publicación:France
Idioma:eng
Resumen:Micropropagated plants experience significant stress from rapid water loss when they are transferred from an in vitro culture to either greenhouse or field conditions. This is caused both by inefficient stomatal control of transpiration and the change to a higher light intensity and lower humidity. Understanding the physiological, vascular and biomechanical processes that allow micropropagated plants to modify their phenotype in response to environmental conditions can help to improve both field performance and plant survival. To identify changes between the hybrid poplar [Populus tremula × (Populus × canescens)] plants propagated from in vitro tissue culture and those from root cuttings, we assessed leaf performance for any differences in leaf growth, photosynthetic and vascular traits, and also nanomechanical properties of the tracheary element cell walls. The micropropagated plants showed significantly higher values for leaf area, leaf length, leaf width and leaf dry mass. The greater leaf area and leaf size dimensions resulted from the higher transpiration rate recorded for this stock type. Also, the micropropagated plants reached higher values for chlorophyll a fluorescence parameters and for the nanomechanical dissipation energy of tracheary element cell walls which may indicate a higher damping capacity within the primary xylem tissue under abiotic stress conditions. The performance of the plants propagated from root cuttings was superior for instantaneous water-use efficiency which signifies a higher acclimation capacity to stressful conditions during a severe drought particularly for this stock type. Similarities were found among the majority of the examined leaf traits for both vegetative plant origins including leaf mass per area, stomatal conductance, net photosynthetic rate, hydraulic axial conductivity, indicators of leaf midrib vascular architecture, as well as for the majority of cell wall nanomechanical traits. This research revealed that there were no drawbacks in the leaf physiological performance which could be attributed to the micropropagated plants of fast growing hybrid poplar.
Tipo de publicación:JOURNAL ARTICLE


  4 / 9654 MEDLINE  
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PMID:29189772
Autor:Ukai H; Kiyonari H; Ueda HR
Dirección:Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center, Osaka, Japan.
Título:Production of knock-in mice in a single generation from embryonic stem cells.
Fuente:Nat Protoc; 12(12):2513-2530, 2017 Dec.
ISSN:1750-2799
País de publicación:England
Idioma:eng
Resumen:The system-level identification and analysis of molecular networks in mammals can be accelerated by 'next-generation' genetics, defined as genetics that does not require crossing of multiple generations of animals in order to achieve the desired genetic makeup. We have established a highly efficient procedure for producing knock-in (KI) mice within a single generation, by optimizing the genome-editing protocol for KI embryonic stem (ES) cells and the protocol for the generation of fully ES-cell-derived mice (ES mice). Using this protocol, the production of chimeric mice is eliminated, and, therefore, there is no requirement for the crossing of chimeric mice to produce mice that carry the KI gene in all cells of the body. Our procedure thus shortens the time required to produce KI ES mice from about a year to ∼3 months. Various kinds of KI ES mice can be produced with a minimized amount of work, facilitating the elucidation of organism-level phenomena using a systems biology approach. In this report, we describe the basic technologies and protocols for this procedure, and discuss the current challenges for next-generation mammalian genetics in organism-level systems biology studies.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:EC 3.1.- (Transcription Activator-Like Effector Nucleases)


  5 / 9654 MEDLINE  
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PMID:28902570
Autor:Rosenbaum L
Dirección:Dr. Rosenbaum is a national correspondent for the Journal.
Título:Tragedy, Perseverance, and Chance - The Story of CAR-T Therapy.
Fuente:N Engl J Med; 377(14):1313-1315, 2017 Oct 05.
ISSN:1533-4406
País de publicación:United States
Idioma:eng
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Antigens, CD19); 0 (Cytokines); 0 (Interleukin-6); 0 (Receptors, Antigen, T-Cell)


  6 / 9654 MEDLINE  
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PMID:28854178
Autor:Vujic A; Bassaneze V; Lee RT
Dirección:Department of Stem Cell and Regenerative Biology and the Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA.
Título:Genetic insights into mammalian heart regeneration.
Fuente:Nat Genet; 49(9):1292-1293, 2017 Aug 30.
ISSN:1546-1718
País de publicación:United States
Idioma:eng
Resumen:Genetic and functional analyses of 120 mouse strains have identified a heart regeneration candidate gene that modulates the contractile sarcomeric apparatus. This gene, Tnni3k, controls the frequency of the mononuclear, diploid cardiomyocyte population, which affects cardiomyocyte proliferative potential after injury.
Tipo de publicación:NEWS


  7 / 9654 MEDLINE  
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PMID:28841417
Autor:Degn SE; van der Poel CE; Firl DJ; Ayoglu B; Al Qureshah FA; Bajic G; Mesin L; Reynaud CA; Weill JC; Utz PJ; Victora GD; Carroll MC
Dirección:Program in Cellular and Molecular Medicine, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Biomedicine, Aarhus University, 8000 Aarhus C, Denmark. Electronic address: sdegn@biomed.au.dk.
Título:Clonal Evolution of Autoreactive Germinal Centers.
Fuente:Cell; 170(5):913-926.e19, 2017 Aug 24.
ISSN:1097-4172
País de publicación:United States
Idioma:eng
Resumen:Germinal centers (GCs) are the primary sites of clonal B cell expansion and affinity maturation, directing the production of high-affinity antibodies. This response is a central driver of pathogenesis in autoimmune diseases, such as systemic lupus erythematosus (SLE), but the natural history of autoreactive GCs remains unclear. Here, we present a novel mouse model where the presence of a single autoreactive B cell clone drives the TLR7-dependent activation, expansion, and differentiation of other autoreactive B cells in spontaneous GCs. Once tolerance was broken for one self-antigen, autoreactive GCs generated B cells targeting other self-antigens. GCs became independent of the initial clone and evolved toward dominance of individual clonal lineages, indicating affinity maturation. This process produced serum autoantibodies to a breadth of self-antigens, leading to antibody deposition in the kidneys. Our data provide insight into the maturation of the self-reactive B cell response, contextualizing the epitope spreading observed in autoimmune disease.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Autoantibodies); 0 (Autoantigens); 0 (Epitopes)


  8 / 9654 MEDLINE  
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Carvalho, Antônio Carlos Campos de
Texto completo SciELO Brasil
PMID:28767980
Autor:Irion CI; Paredes BD; Brasil GV; Cunha STD; Paula LF; Carvalho AR; Carvalho ACC; Carvalho AB; Goldenberg RCDS
Dirección:Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
Título:Bone marrow cell migration to the heart in a chimeric mouse model of acute chagasic disease.
Fuente:Mem Inst Oswaldo Cruz; 112(8):551-560, 2017 Aug.
ISSN:1678-8060
País de publicación:Brazil
Idioma:eng
Resumen:BACKGROUND: Chagas disease is a public health problem caused by infection with the protozoan Trypanosoma cruzi. There is currently no effective therapy for Chagas disease. Although there is some evidence for the beneficial effect of bone marrow-derived cells in chagasic disease, the mechanisms underlying their effects in the heart are unknown. Reports have suggested that bone marrow cells are recruited to the chagasic heart; however, studies using chimeric mouse models of chagasic cardiomyopathy are rare. OBJECTIVES: The aim of this study was to investigate the migration of bone marrow cells to the heart after T. cruzi infection in a model of chagasic disease in chimeric mice. METHODS: To obtain chimerical mice, wild-type (WT) C57BL6 mice were exposed to full body irradiation (7 Gy), causing bone marrow ablation. Then, bone marrow cells from green fluorescent protein (GFP)-transgenic mice were infused into the mice. Graft effectiveness was confirmed by flow cytometry. Experimental mice were divided into four groups: (i) infected chimeric (iChim) mice; (ii) infected WT (iWT) mice, both of which received 3 × 104 trypomastigotes of the Brazil strain; (iii) non-infected chimeric (Chim) mice; and (iv) non-infected WT mice. FINDINGS: At one-month post-infection, iChim and iWT mice showed first degree atrioventricular block with decreased heart rate and treadmill exercise parameters compared to those in the non-infected groups. MAIN CONCLUSIONS: iChim mice showed an increase in parasitaemia, myocarditis, and the presence of amastigote nests in the heart tissue compared to iWT mice. Flow cytometry analysis did not detect haematopoietic progenitor cells in the hearts of infected mice. Furthermore, GFP+ cardiomyocytes were not detected in the tissues of chimeric mice.
Tipo de publicación:JOURNAL ARTICLE


  9 / 9654 MEDLINE  
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PMID:28733170
Autor:Dondorp W; Johnson MH
Título:Human-animal chimeras: circumventing rather than discussing ethical concerns comes at a price.
Fuente:Reprod Biomed Online; 35(4):341-342, 2017 10.
ISSN:1472-6491
País de publicación:Netherlands
Idioma:eng
Tipo de publicación:EDITORIAL; COMMENT


  10 / 9654 MEDLINE  
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PMID:28659616
Autor:Barzi M; Pankowicz FP; Zorman B; Liu X; Legras X; Yang D; Borowiak M; Bissig-Choisat B; Sumazin P; Li F; Bissig KD
Dirección:Center for Cell and Gene Therapy, Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, TX-77030, USA.
Título:A novel humanized mouse lacking murine P450 oxidoreductase for studying human drug metabolism.
Fuente:Nat Commun; 8(1):39, 2017 06 28.
ISSN:2041-1723
País de publicación:England
Idioma:eng
Resumen:Only one out of 10 drugs in development passes clinical trials. Many fail because experimental animal models poorly predict human xenobiotic metabolism. Human liver chimeric mice are a step forward in this regard, as the human hepatocytes in chimeric livers generate human metabolites, but the remaining murine hepatocytes contain an expanded set of P450 cytochromes that form the major class of drug-metabolizing enzymes. We therefore generated a conditional knock-out of the NADPH-P450 oxidoreductase (Por) gene combined with Il2rg /Rag2 /Fah (PIRF) mice. Here we show that homozygous PIRF mouse livers are readily repopulated with human hepatocytes, and when the murine Por gene is deleted (<5%), they predominantly use human cytochrome metabolism. When given the anticancer drug gefitinib or the retroviral drug atazanavir, the Por-deleted humanized PIRF mice develop higher levels of the major human metabolites than current models. Humanized, murine Por-deficient PIRF mice can thus predict human drug metabolism and should be useful for preclinical drug development.Human liver chimeric mice are increasingly used for drug testing in preclinical development, but express residual murine p450 cytochromes. Here the authors generate mice lacking the Por gene in the liver, and show that human cytochrome metabolism is used following repopulation with human hepatocytes.
Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
Nombre de substancia:0 (Antineoplastic Agents); 0 (Cytochromes); 0 (HIV Protease Inhibitors); 0 (Quinazolines); 4MT4VIE29P (Atazanavir Sulfate); 9035-51-2 (Cytochrome P-450 Enzyme System); S65743JHBS (gefitinib)



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