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  1 / 25340 MEDLINE  
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PMID:29351866
Autor:Konieczna L; Roszkowska A; Stachowicz-Stencel T; Synakiewicz A; Baczek T
Dirección:Department of Pharmaceutical Chemistry, Medical University of Gdansk, Hallera 107 Str., 80-416 Gdansk, Poland.
Título:Bioanalysis of a panel of neurotransmitters and their metabolites in plasma samples obtained from pediatric patients with neuroblastoma and Wilms' tumor.
Fuente:J Chromatogr B Analyt Technol Biomed Life Sci; 1074-1075:99-110, 2018 Feb 01.
ISSN:1873-376X
País de publicación:Netherlands
Idioma:eng
Resumen:This paper details the quantitative analysis of neurotransmitters, including dopamine (DA), norepinephrine (NE), epinephrine (E), and serotonin (5-HT), along with their respective precursors and metabolites in children with solid tumors: Wilms' tumor (WT) and neuroblastoma (NB). A panel of neurotransmitters was determined with the use of dispersive liquid-liquid microextraction (DLLME) technique combined with liquid-chromatography mass spectrometry (LC-MS/MS) in plasma samples obtained from a group of pediatric subjects with solid tumors and a control group of healthy children. Next, statistical univariate analysis (t-test) and multivariate analysis (Principal Component Analysis) were performed using chromatographic data. The levels of tyrosine (Tyr) and tryptophan (Trp) (the precursors of analyzed neurotransmitters) as well as 3,4-dihydroxyphenylacetic acid (DOPAC) (a product of metabolism of DA) were significantly higher in the plasma samples obtained from pediatric patients with WT than in the samples taken from the control group. Moreover, statistically significant differences were observed between the levels of 5-HT and homovanillic acid (HVA) in the plasma samples from pediatric patients with solid tumors and the control group. However, elevated levels of these analytes did not facilitate a clear distinction between pediatric patients with WT and those with NB. Nonetheless, the application of advanced statistical tools allowed the healthy controls to be differentiated from the pediatric oncological patients. The identification and quantification of a panel of neurotransmitters as potential prognostic factors in selected childhood malignancies may provide clinically relevant information about ongoing metabolic alterations, and it could potentially serve as an adjunctive strategy in the effective diagnosis and treatment of solid tumors in children.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Neurotransmitter Agents)


  2 / 25340 MEDLINE  
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PMID:29378209
Autor:Zhu M; Tian Y; Zhang H; Ma X; Shang B; Zhang J; Jiao Y; Zhang Y; Hu J; Wang Y
Dirección:Department of Psychiatry and Psychology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
Título:Methylphenidate ameliorates hypoxia-induced mitochondrial damage in human neuroblastoma SH-SY5Y cells through inhibition of oxidative stress.
Fuente:Life Sci; 197:40-45, 2018 Mar 15.
ISSN:1879-0631
País de publicación:Netherlands
Idioma:eng
Resumen:AIMS: Methylphenidate (MPH) is a dopamine-reuptake inhibitor approved for the treatment of attention-deficit/hyperactivity disorder (ADHD). Nonetheless, the cellular and molecular mechanisms of MPH are still unknown. We attempt to determine whether MPH protect neuron cells against oxidative stress by using human neuroblastoma SH-SY5Y cells. MAIN METHODS: The SH-SY5Y cells were cultured in normoxic and hypoxic conditions in the presence of different doses of MPH. Then, reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and adenosine triphosphate (ATP) production were quantitatively measured by using flow cytometry or spectrophotometry. The mitochondrial ultrastructure of the cells was observed by electron microscope, and the function of mitochondrial was evaluated by measuring mitochondrial membrane potential (MMP) using flow cytometry. The levels of SOD and heme oxygenase-1 (HO-1) proteins were detected by Western blot. KEY FINDINGS: We found that low doses of MPH treatment (50-500 ng/mL) led to decreased ROS and MDA production (P<0.05), increased GSH and SOD as well as ATP concentration (P<0.05) in hypoxic SH-SY5Y cells. Additionally, low doses of MPH significantly inhibited mitochondrial swelling and decreased the percentage of JC-1 monomer positive cells. However, we did not observe the same effects of MPH in normoxia. SIGNIFICANCE: Our results show that low doses of MPH play protective roles in maintaining mitochondrial homeostasis in response to hypoxia-induced oxidative stress. Our findings may provide novel insight into the mechanisms of MPH in the treatment of ADHD, and shed light on the disease mechanisms of ADHD.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Neoplasm Proteins); 0 (Reactive Oxygen Species); 207ZZ9QZ49 (Methylphenidate); 8L70Q75FXE (Adenosine Triphosphate); EC 1.14.14.18 (HMOX1 protein, human); EC 1.14.14.18 (Heme Oxygenase-1); EC 1.15.1.1 (Superoxide Dismutase)


  3 / 25340 MEDLINE  
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PMID:29390274
Autor:Yuan LQ; Wang JH; Zhu K; Yang M; Gu WZ; Lai C; Li HM; Shu Q; Chen X
Dirección:Departments of Central Laboratory, Pathology, Oncology and Radiology, The Children's Hospital of Zhejiang University School of Medicine.
Título:A highly malignant case of neuroblastoma with substantial increase of single-nucleotide variants and normal mismatch repair system: A case report.
Fuente:Medicine (Baltimore); 96(50):e8845, 2017 Dec.
ISSN:1536-5964
País de publicación:United States
Idioma:eng
Resumen:RATIONALE: Neuroblastoma is a common abdominal malignancy in children. The chemoresistant and relapsed cases have poor prognosis. The genetic background and the mechanism of resistance remain unelucidated. Next-generation sequence (NGS) is becoming a popular tool to unravel the genetic background and to guide precision medicine in oncology studies as well as in clinical practice. PATIENT CONCERNS: Here we report a neuroblastoma case of a boy aged 2 years and 8 months when first diagnosed, with multiple metastatic sites found in both lungs. The metastatic tumors were resistant to chemotherapy and the patient suffered from severe bone marrow suppression. NGS of the whole exon revealed somatic mutations including 9666 single-nucleotide variants (SNVs) from 5148 genes, 55 copy number variations (CNVs), and 140 insertion-deletion variations. The high frequency of SNVs makes it distinguished case. However, no mutation of key tumor driver genes with functional significance was identified. No abnormality was found in nucleic acid synthesis enzymes. No amplification of c-Myc and n-Myc was found by fluorescence in situ hybridization (FISH). Both NGS and immunohistochemistry (IHC) analysis indicated that DNA mismatch repair (MMR) system was intact. INTERVENTIONS: After initial diagnosis, the patient received combinational chemotherapy, which includes vindesine, an analogue of adriamycin suggested by NGS data, for 4 months. Radical section of the tumor together with the left kidney and the left adrenal gland was performed 5 months after diagnosis. Postsurgical chemotherapy protocols was similar with the previous. OUTCOMES: The patient died 2 years after initial diagnosis after 8 relapses following combinational chemotherapy. LESSONS: This case of neuroblastoma is with pronounced somatic mutations but unidentified driver gene and therapeutic target. Although NGS is a potentially powerful tool to guide precision medicine, at current stage, its application in the clinic certainly has its limits. The underlying mechanism of the substantially increased SNV number, as well as the malignant behaviors of the tumor, is yet to be revealed.
Tipo de publicación:CASE REPORTS; JOURNAL ARTICLE


  4 / 25340 MEDLINE  
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PMID:29342186
Autor:Hultman I; Haeggblom L; Rognmo I; Jansson Edqvist J; Blomberg E; Ali R; Phillips L; Sandstedt B; Kogner P; Shirazi Fard S; Ährlund-Richter L
Dirección:Department of Women's and Children's Health, Karolinska Institutet, Stockholm. Sweden.
Título:Doxorubicin-provoked increase of mitotic activity and concomitant drain of G0-pool in therapy-resistant BE(2)-C neuroblastoma.
Fuente:PLoS One; 13(1):e0190970, 2018.
ISSN:1932-6203
País de publicación:United States
Idioma:eng
Resumen:In this study chemotherapy response in neuroblastoma (NB) was assessed for the first time in a transplantation model comprising non-malignant human embryonic microenvironment of pluripotent stem cell teratoma (PSCT) derived from diploid bona fide hESC. Two NB cell lines with known high-risk phenotypes; the multi-resistant BE(2)-C and the drug sensitive IMR-32, were transplanted to the PSCT model and the tumour growth was exposed to single or repeated treatments with doxorubicin, and thereafter evaluated for cell death, apoptosis, and proliferation. Dose dependent cytotoxic effects were observed, this way corroborating the experimental platform for this type of analysis. Notably, analysis of doxorubicin-resilient BE(2)-C growth in the PSCT model revealed an unexpected 1,5-fold increase in Ki67-index (p<0.05), indicating that non-cycling (G0) cells entered the cell cycle following the doxorubicin exposure. Support for this notion was obtained also in vitro. A pharmacologically relevant dose (1µM) resulted in a marked accumulation of Ki67 positive BE(2)-C cells (p<0.0001), as well as a >3-fold increase in active cell cycle (i.e. cells positive staining for PH3 together with incorporation of EdU) (p<0.01). Considering the clinical challenge for treating high-risk NB, the discovery of a therapy-provoked growth-stimulating effect in the multi-resistant and p53-mutated BE(2)-C cell line, but not in the drug-sensitive p53wt IMR-32 cell line, warrants further studies concerning generality and clinical significance of this new observation.
Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Nombre de substancia:80168379AG (Doxorubicin)


  5 / 25340 MEDLINE  
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PMID:27776337
Autor:Chlenski A; Dobratic M; Salwen HR; Applebaum M; Guerrero LJ; Miller R; DeWane G; Solomaha E; Marks JD; Cohn SL
Dirección:Department of Pediatrics, University of Chicago, Chicago, IL, USA.
Título:Secreted protein acidic and rich in cysteine (SPARC) induces lipotoxicity in neuroblastoma by regulating transport of albumin complexed with fatty acids.
Fuente:Oncotarget; 7(47):77696-77706, 2016 Nov 22.
ISSN:1949-2553
País de publicación:United States
Idioma:eng
Resumen:SPARC is a matrix protein that mediates interactions between cells and the microenvironment. In cancer, SPARC may either promote or inhibit tumor growth depending upon the tumor type. In neuroblastoma, SPARC is expressed in the stromal Schwannian cells and functions as a tumor suppressor. Here, we developed a novel in vivo model of stroma-rich neuroblastoma using non-tumorigenic SHEP cells with modulated levels of SPARC, mixed with tumorigenic KCNR cells. Tumors with stroma-derived SPARC displayed suppressed growth, inhibited angiogenesis and increased lipid accumulation. Based on the described chaperone function of SPARC, we hypothesized that SPARC binds albumin complexed with fatty acids and transports them to tumors. We show that SPARC binds albumin with Kd=18.9±2.3 uM, and enhances endothelial cell internalization and transendothelial transport of albumin in vitro. We also demonstrate that lipids induce toxicity in neuroblastoma cells and show that lipotoxicity is increased when cells are cultured in hypoxic conditions. Studies investigating the therapeutic potential of SPARC are warranted.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Osteonectin); 0 (SPARC protein, human); 27432CM55Q (Serum Albumin, Bovine); 2V16EO95H1 (Palmitic Acid)


  6 / 25340 MEDLINE  
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PMID:29374712
Autor:Kurita M; Takada T; Wakabayashi N; Asami S; Ono S; Uchiyama T; Suzuki T
Dirección:Department of Clinical Medicine, School of Pharmacy, Nihon University, Funabashi, Japan.
Título:Antitumor Effect of Burchellin Derivatives Against Neuroblastoma.
Fuente:Anticancer Res; 38(2):855-862, 2018 02.
ISSN:1791-7530
País de publicación:Greece
Idioma:eng
Resumen:BACKGROUND: Neuroblastoma is one of the most commonly encountered malignant solid tumors in the pediatric age group. We examined the antitumor effects of five burchellin derivatives against human neuroblastoma cell lines. MATERIALS AND METHODS: We evaluated cytotoxicity by the MTT assay for four human neuroblastoma and two normal cell lines. We also performed analysis of the apoptotic induction effect by flow cytometry, and examined the expression levels of apoptosis- and cell growth-related proteins by western blot analysis. RESULTS: We found that one of the burchellin derivatives (compound ) exerted cytotoxicity against the neuroblastoma cell lines. Compound induced caspase-dependent apoptosis via a mitochondrial pathway. The apoptosis mechanisms induced by compound involved caspase-3, -7 and -9 activation and poly (ADP-ribose) polymerase cleavage. In addition, compound induced cell death through inhibition of the cell growth pathway (via extracellular signal-regulated kinase 1 and 2, AKT8 virus oncogene cellular homolog, and signal transducer and activator of transcription 3). CONCLUSION: Compound exerted cellular cytotoxicity against neuroblastoma cells via induction of caspase-dependent apoptosis, and may offer promise for further development as a useful drug for the treatment of advanced neuroblastoma.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Antineoplastic Agents, Phytogenic); 0 (Benzofurans); 0 (Drugs, Chinese Herbal); 38276-59-4 (burchellin)


  7 / 25340 MEDLINE  
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PMID:29406108
Autor:Xu T; Xie HQ; Li Y; Xia Y; Sha R; Wang L; Chen Y; Xu L; Zhao B
Dirección:State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100085, China. Electronic address: xutuan2012@163.com.
Título:Dioxin induces expression of hsa-miR-146b-5p in human neuroblastoma cells.
Fuente:J Environ Sci (China); 63:260-267, 2018 Jan.
ISSN:1001-0742
País de publicación:Netherlands
Idioma:eng
Resumen:Dioxin can cause a series of neural toxicological effects. MicroRNAs (miRs) play important roles in regulating nervous system function and mediating cellular responses to environmental pollutants, such as dioxin. Hsa-miR-146b-5p appears to be involved in neurodegenerative diseases and brain tumors. However, little is known about effects of dioxin on the expression of hsa-miR-146b-5p. We found that the hsa-miR-146b-5p expression and its promoter activity were significantly increased in dioxin treated SK-N-SH cells, a human-derived neuroblastoma cell line. Potential roles of hsa-miR-146b-5p in mediating neural toxicological effects of dioxin may be due to the regulation of certain target genes. We further confirmed that hsa-miR-146b-5p significantly suppressed acetylcholinesterase (AChE) activity and targeted the 3'-untranslated region of the AChE T subunit, which has been down-regulated in dioxin treated SK-N-SH cells. Functional bioinformatic analysis showed that the known and predicted target genes of hsa-miR-146b-5p were involved in some brain functions or cyto-toxicities related to known dioxin effects, including synapse transmission, in which AChE may serve as a responsive gene for mediating the effect.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Dioxins); 0 (Environmental Pollutants); 0 (MIRN146 microRNA, human); 0 (MicroRNAs); EC 3.1.1.7 (Acetylcholinesterase)


  8 / 25340 MEDLINE  
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PMID:29316911
Autor:Caputi FF; Acquas E; Kasture S; Ruiu S; Candeletti S; Romualdi P
Dirección:Department of Pharmacy and Biotechnology, Alma Mater Studiorum - University of Bologna, Via Irnerio 48, 40126, Bologna, Italy. francesca.caputi3@unibo.it.
Título:The standardized Withania somnifera Dunal root extract alters basal and morphine-induced opioid receptor gene expression changes in neuroblastoma cells.
Fuente:BMC Complement Altern Med; 18(1):9, 2018 Jan 10.
ISSN:1472-6882
País de publicación:England
Idioma:eng
Resumen:BACKGROUND: Behavioral studies demonstrated that the administration of Withania somnifera Dunal roots extract (WSE), prolongs morphine-elicited analgesia and reduces the development of tolerance to the morphine's analgesic effect; however, little is known about the underpinning molecular mechanism(s). In order to shed light on this issue in the present paper we explored whether WSE promotes alterations of µ (MOP) and nociceptin (NOP) opioid receptors gene expression in neuroblastoma SH-SY5Y cells. METHODS: A range of WSE concentrations was preliminarily tested to evaluate their effects on cell viability. Subsequently, the effects of 5 h exposure to WSE (0.25, 0.50 and 1.00 mg/ml), applied alone and in combination with morphine or naloxone, on MOP and NOP mRNA levels were investigated. RESULTS: Data analysis revealed that morphine decreased MOP and NOP receptor gene expression, whereas naloxone elicited their up-regulation. In addition, pre-treatment with naloxone prevented the morphine-elicited gene expression alterations. Interestingly, WSE was able to: a) alter MOP but not NOP gene expression; b) counteract, at its highest concentration, morphine-induced MOP down-regulation, and c) hamper naloxone-induced MOP and NOP up-regulation. CONCLUSION: Present in-vitro data disclose novel evidence about the ability of WSE to influence MOP and NOP opioid receptors gene expression in SH-SY5Y cells. Moreover, our findings suggest that the in-vivo modulation of morphine-mediated analgesia by WSE could be related to the hindering of morphine-elicited opioid receptors down-regulation here observed following WSE pre-treatment at its highest concentration.
Tipo de publicación:JOURNAL ARTICLE
Nombre de substancia:0 (Plant Extracts); 0 (Receptors, Opioid)


  9 / 25340 MEDLINE  
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PMID:28792662
Autor:Ezekian B; Englum BR; Gulack BC; Rialon KL; Kim J; Talbot LJ; Adibe OO; Routh JC; Tracy ET; Rice HE
Dirección:Department of Surgery, Duke University Medical Center, Durham, North Carolina.
Título:Comparing oncologic outcomes after minimally invasive and open surgery for pediatric neuroblastoma and Wilms tumor.
Fuente:Pediatr Blood Cancer; 65(1), 2018 Jan.
ISSN:1545-5017
País de publicación:United States
Idioma:eng
Resumen:BACKGROUND: Minimally invasive surgery (MIS) has been widely adopted for common operations in pediatric surgery; however, its role in childhood tumors is limited by concerns about oncologic outcomes. We compared open and MIS approaches for pediatric neuroblastoma and Wilms tumor (WT) using a national database. METHODS: The National Cancer Data Base from 2010 to 2012 was queried for cases of neuroblastoma and WT in children ≤21 years old. Children were classified as receiving open or MIS surgery for definitive resection, with clinical outcomes compared using a propensity matching methodology (two open:one MIS). RESULTS: For children with neuroblastoma, 17% (98 of 579) underwent MIS, while only 5% of children with WT (35 of 695) had an MIS approach for tumor resection. After propensity matching, there was no difference between open and MIS surgery for either tumor for 30-day mortality, readmissions, surgical margin status, and 1- and 3-year survival. However, in both tumors, open surgery more often evaluated lymph nodes and had larger lymph node harvest. CONCLUSION: Our retrospective review suggests that the use of MIS appears to be a safe method of oncologic resection for select children with neuroblastoma and WT. Further research should clarify which children are the optimal candidates for this approach.
Tipo de publicación:COMPARATIVE STUDY; JOURNAL ARTICLE


  10 / 25340 MEDLINE  
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PMID:29307179
Autor:Morroni F; Sita G; Djemil A; D'Amico M; Pruccoli L; Cantelli-Forti G; Hrelia P; Tarozzi A
Dirección:Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna , Bologna, Italy.
Título:Comparison of Adaptive Neuroprotective Mechanisms of Sulforaphane and its Interconversion Product Erucin in in Vitro and in Vivo Models of Parkinson's Disease.
Fuente:J Agric Food Chem; 66(4):856-865, 2018 Jan 31.
ISSN:1520-5118
País de publicación:United States
Idioma:eng
Resumen:Several studies suggest that an increase of glutathione (GSH) through activation of the transcriptional nuclear factor (erythroid-derived 2)-like 2 (Nrf2) in the dopaminergic neurons may be a promising neuroprotective strategy in Parkinson's disease (PD). Among Nrf2 activators, isothiocyanate sulforaphane (SFN), derived from precursor glucosinolate present in Brassica vegetables, has gained attention as a potential neuroprotective compound. Bioavailability studies also suggest the contribution of SFN metabolites, including erucin (ERN), to the neuroprotective effects of SFN. Therefore, we compared the in vitro neuroprotective effects of SFN and ERN at the same dose level (5 µM) and oxidative treatment with 6-hydroxydopamine (6-OHDA) in SH-SY5Y cells. The pretreatment of SH-SY5Y cells with SFN recorded a higher (p < 0.05) active nuclear Nrf2 protein (12.0 ± 0.4 vs 8.0 ± 0.2 fold increase), mRNA Nrf2 (2.0 ± 0.3 vs 1.4 ± 0.1 fold increase), total GSH (384.0 ± 9.0 vs 256.0 ± 8.0 µM) levels, and resistance to neuronal apoptosis elicited by 6-OHDA compared to ERN. By contrast, the simultaneous treatment of SH-SY5Y cells with either SFN or ERN and 6-OHDA recorded similar neuroprotective effects with both the isothiocyanates (Nrf2 protein 2.2 ± 0.2 vs 2.1 ± 0.1 and mRNA Nrf2 2.1 ± 0.3 vs 1.9 ± 0.2 fold increase; total GSH 384.0 ± 4.8 vs 352.0 ± 6.4 µM). Finally, in vitro finding was confirmed in a 6-OHDA-PD mouse model. The metabolic oxidation of ERN to SFN could account for their similar neuroprotective effects in vivo, raising the possibility of using vegetables containing a precursor of ERN for systemic antioxidant benefits in a similar manner to SFN.
Tipo de publicación:COMPARATIVE STUDY; JOURNAL ARTICLE
Nombre de substancia:0 (Isothiocyanates); 0 (NF-E2-Related Factor 2); 0 (NFE2L2 protein, human); 0 (Neuroprotective Agents); 0 (RNA, Messenger); 0 (Sulfides); 0 (Thiocyanates); 8HW4YBZ748 (Oxidopamine); CTE370DL3U (erucin); GA49J4310U (sulforafan); GAN16C9B8O (Glutathione)



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