Database : MEDLINE
Search on : D02.241.081.337.463.703 [DeCS Category]
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  1 / 211 MEDLINE  
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PMID:28358882
Author:Meinert C; Brandt U; Heine V; Beyert J; Schmidl S; Wübbeler JH; Voigt B; Riedel K; Steinbüchel A
Address:Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, Münster, Germany.
Title:Proteomic analysis of organic sulfur compound utilisation in Advenella mimigardefordensis strain DPN7T.
Source:PLoS One; 12(3):e0174256, 2017.
ISSN:1932-6203
Country of publication:United States
Language:eng
Abstract:2-Mercaptosuccinate (MS) and 3,3´-ditiodipropionate (DTDP) were discussed as precursor substance for production of polythioesters (PTE). Therefore, degradation of MS and DTDP was investigated in Advenella mimigardefordensis strain DPN7T, applying differential proteomic analysis, gene deletion and enzyme assays. Protein extracts of cells cultivated with MS, DTDP or 3-sulfinopropionic acid (SP) were compared with those cultivated with propionate (P) and/or succinate (S). The chaperone DnaK (ratio DTDP/P 9.2, 3SP/P 4.0, MS/S 6.1, DTDP/S 6.2) and a Do-like serine protease (DegP) were increased during utilization of all organic sulfur compounds. Furthermore, a putative bacterioferritin (locus tag MIM_c12960) showed high abundance (ratio DTDP/P 5.3, 3SP/P 3.2, MS/S 4.8, DTDP/S 3.9) and is probably involved in a thiol-specific stress response. The deletion of two genes encoding transcriptional regulators (LysR (MIM_c31370) and Xre (MIM_c31360)) in the close proximity of the relevant genes of DTDP catabolism (acdA, mdo and the genes encoding the enzymes of the methylcitric acid cycle; prpC,acnD, prpF and prpB) showed that these two regulators are essential for growth of A. mimigardefordensis strain DPN7T with DTDP and that they most probably regulate transcription of genes mandatory for this catabolic pathway. Furthermore, proteome analysis revealed a high abundance (ratio MS/S 10.9) of a hypothetical cupin-2-domain containing protein (MIM_c37420). This protein shows an amino acid sequence similarity of 60% to a newly identified MS dioxygenase from Variovorax paradoxus strain B4. Deletion of the gene and the adjacently located transcriptional regulator LysR, as well as heterologous expression of MIM_c37420, the putative mercaptosuccinate dioxygenase (Msdo) from A. mimigardefordensis, showed that this protein is the key enzyme of MS degradation in A. mimigardefordensis strain DPN7T (KM 0.2 mM, specific activity 17.1 µmol mg-1 min-1) and is controlled by LysR (MIM_c37410).
Publication type:JOURNAL ARTICLE
Name of substance:0 (Organic Chemicals); 0 (Propionates); 0 (Proteome); 0 (Thiomalates); 52329-65-4 (3-sulfinopropionic acid); 94239W5L4H (2-thiomalic acid)


  2 / 211 MEDLINE  
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PMID:27398533
Author:Chen N; Chen J; Yang JH; Bai LY; Zhang YP
Title:Colorimetric Detection of Cadmium Ions Using DL-Mercaptosuccinic Acid-Modified Gold Nanoparticles.
Source:J Nanosci Nanotechnol; 16(1):840-3, 2016 Jan.
ISSN:1533-4880
Country of publication:United States
Language:eng
Abstract:A colorimetric assay has been developed for detection of Cd²âº utilizing DL-mercaptosuccinic acid-modified gold nanoparticles (MSA-AuNPs). The method showed good selectivity for Cd²âº over other metal ions. As a result, the linear relationships (r > 0.9606) between concentration 0.07 mM and 0.20 mM for cadmium ion were obtained. The detection limit was as low as 0.07 mM by the naked eye. The effect of pH on the aggregation was optimized. The MSA-AuNPs probe could be used to detect Cd²âº in an aqueous solution based on the aggregation-induced color change of MSA-AuNPs.
Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Name of substance:0 (Ions); 0 (Thiomalates); 00BH33GNGH (Cadmium); 7440-57-5 (Gold); 94239W5L4H (2-thiomalic acid)


  3 / 211 MEDLINE  
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PMID:27151673
Author:Bao L; Zhu X; Dai H; Tao Y; Zhou X; Liu W; Kong Y
Address:Advanced Catalysis and Green Manufacturing Collaborative Innovation Center, School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China.
Title:Synthesis of porous starch xerogels modified with mercaptosuccinic acid to remove hazardous gardenia yellow.
Source:Int J Biol Macromol; 89:389-95, 2016 Aug.
ISSN:1879-0003
Country of publication:Netherlands
Language:eng
Abstract:Mercaptosuccinic acid (MSA) molecules were inserted into potato starch, leading to the breaking of intrinsic H-bonds within macromolecular chains of starch and the formation of intermolecular H-bonds between MSA and starch, which could be verified by Fourier transform infrared spectroscopy (FT-TR). MSA modified porous starch xerogels (PSX/MSA) were obtained after freeze-drying the MSA modified starch, and they were characterized by field emission scanning electron microscopy (FESEM), exhibiting the intriguing porous structure due to the separation of starch chains by MSA molecules. The PSX/MSA were then used as the adsorbents to remove gardenia yellow (GY), a natural colorant with genotoxicity. Due to the porous structure of PSX and the introduced carboxyl groups from MSA, the adsorption capacity of the PSX/MSA was much higher than that of the starch xerogels alone (SX). The adsorption behaviors of GY by the PSX/MSA fitted both the Freundlich isotherm model and the pseudo-second-order kinetic model, and the efficient adsorption of GY suggested that the PSX/MSA might be potential adsorbents for the removal of dyes from contaminated aquatic systems.
Publication type:JOURNAL ARTICLE
Name of substance:0 (Coloring Agents); 0 (Plant Extracts); 0 (Thiomalates); 0 (Water Pollutants, Chemical); 9005-25-8 (Starch); 94239W5L4H (2-thiomalic acid); F32BA2H92Z (gardenia yellow)


  4 / 211 MEDLINE  
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PMID:26893560
Author:Yan M; Zhang Y; Qin H; Liu K; Guo M; Ge Y; Xu M; Sun Y; Zheng X
Address:Department of Biomedical Engineering, College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou, People's Republic of China.
Title:Cytotoxicity of CdTe quantum dots in human umbilical vein endothelial cells: the involvement of cellular uptake and induction of pro-apoptotic endoplasmic reticulum stress.
Source:Int J Nanomedicine; 11:529-42, 2016.
ISSN:1178-2013
Country of publication:New Zealand
Language:eng
Abstract:Cadmium telluride quantum dots (CdTe QDs) have been proposed to induce oxidative stress, which plays a crucial role in CdTe QDs-mediated mitochondrial-dependent apoptosis in human umbilical vein endothelial cells (HUVECs). However, the direct interactions of CdTe QDs with HUVECs and their potential impairment of other organelles like endoplasmic reticulum (ER) in HUVECs are poorly understood. In this study, we reported that the negatively charged CdTe QDs (-21.63±0.91 mV), with good dispersity and fluorescence stability, were rapidly internalized via endocytosis by HUVECs, as the notable internalization could be inhibited up to 95.52% by energy depletion (NaN3/deoxyglucose or low temperature). The endocytosis inhibitors (methyl-ß-cyclodextrin, genistein, sucrose, chlorpromazine, and colchicine) dramatically decreased the uptake of CdTe QDs by HUVECs, suggesting that both caveolae/raft- and clathrin-mediated endocytosis were involved in the endothelial uptake of CdTe QDs. Using immunocytochemistry, a striking overlap of the internalized CdTe QDs and ER marker was observed, which indicates that QDs may be transported to ER. The CdTe QDs also caused remarkable ER stress responses in HUVECs, confirmed by significant dilatation of ER cisternae, upregulation of ER stress markers GRP78/GRP94, and activation of protein kinase RNA-like ER kinase-eIF2α-activating transcription factor 4 pathway (including phosphorylation of both protein kinase RNA-like ER kinase and eIF2α and elevated level of activating transcription factor 4). CdTe QDs further promoted an increased C/EBP homologous protein expression, phosphorylation of c-JUN NH2-terminal kinase, and cleavage of ER-resident caspase-4, while the specific inhibitor (SP600125, Z-LEVD-fmk, or salubrinal) significantly attenuated QDs-triggered apoptosis, indicating that all three ER stress-mediated apoptosis pathways were activated and the direct participation of ER in the CdTe QDs-caused apoptotic cell death in HUVECs. Our findings provide important new insights into QDs toxicity and reveal potential cardiovascular risks for the future applications of QDs.
Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Name of substance:0 (Cadmium Compounds); 0 (Heat-Shock Proteins); 0 (Thiomalates); 0 (beta-Cyclodextrins); 0 (methyl-beta-cyclodextrin); 0 (molecular chaperone GRP78); 94239W5L4H (2-thiomalic acid); DH2M523P0H (Genistein); EC 2.7.11.1 (eIF-2 Kinase); NQA0O090ZJ (Tellurium); SML2Y3J35T (Colchicine); STG188WO13 (cadmium telluride)


  5 / 211 MEDLINE  
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PMID:26743581
Author:Liang X; Wang H; Zhu Y; Zhang R; Cogger VC; Liu X; Xu ZP; Grice JE; Roberts MS
Address:Therapeutics Research Centre, School of Medicine, The University of Queensland , Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia.
Title:Short- and Long-Term Tracking of Anionic Ultrasmall Nanoparticles in Kidney.
Source:ACS Nano; 10(1):387-95, 2016 Jan 26.
ISSN:1936-086X
Country of publication:United States
Language:eng
Abstract:While biodistribution of nanoparticles (NPs) has been widely studied at the organ level, relatively little is known about their disposition in organs at the cellular level, especially after long-term exposure. The kidney is regarded as the key organ for the clearance of ultrasmall NPs (<5.5 nm). However, recent studies indicate that NPs in this size range could accumulate in the kidney for extended times without urinary excretion. Using negatively charged quantum dots (QDs) (∼3.7 nm) as a model system, we examined the suborgan disposition of anionic ultrasmall NPs in the kidney at the cellular level after intravenous injection by multiphoton microscopy coupled with fluorescence lifetime imaging. Most of the NPs were initially distributed in the peritubular capillaries or glomerular arterioles after injection, whereas they passed through the fenestrated glomerular endothelium and were gradually taken up by mesangial cells up to 30 days after injection. Only trace amounts of anionic QDs could be detected in the urine, which could be attributed to the barrier of the anionic glomerular basement membrane preventing filtration of anionic QDs. In contrast, cationic QDs of similar size (∼5.67 nm) were found to be readily excreted into urine. This study thus highlights the importance of surface charge in determining renal clearance of ultrasmall NPs. It provides a framework for characterizing and predicting the subcellular disposition in organs and long-term targeting of other NPs, with a physiologically based kinetic model being subsequently developed to describe the suborgan kinetics of anionic ultrasmall NPs.
Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Name of substance:0 (Anions); 0 (Cations); 0 (Thiomalates); 27432CM55Q (Serum Albumin, Bovine); 9002-98-6 (Polyethyleneimine); 94239W5L4H (2-thiomalic acid)


  6 / 211 MEDLINE  
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PMID:26301311
Author:Rekha MR; Sharma CP
Title:Simultaneous Effect of Thiolation and Carboxylation of Chitosan Particles Towards Mucoadhesive Oral Insulin Delivery Applications: An In Vitro and In Vivo Evaluation.
Source:J Biomed Nanotechnol; 11(1):165-76, 2015 Jan.
ISSN:1550-7033
Country of publication:United States
Language:eng
Abstract:Thiomalyl chitosan (TCS), a pH sensitive thiolated chitosan derivative, was developed and investigated towards oral protein delivery application. Particles of z-average 364 ± 5.6 nm with a negative zeta potential of 14.4 mV was obtained by tripolyphosphate cross linking of TCS. The release of insulin from TCS particles was significantly restricted at pH 1.2 minimizing up to about < 10% in 3 hours. The permeation enhancement ratio was found to 13 times higher than the FD4 alone and was 1.6 times higher than the unmodified chitosan particles. The protein protective properties of the matrix were established in presence of pepsin and pancreatic enzymes. Confocal microscopy studies proved the tight junction opening of Caco-2 cells by these thiolated chitosan particles and the in vivo studies on diabetic rats established its potential towards oral peptide delivery with pharmacological availability (PA) of 1.5%. The significance of this work is to establish that, the presence of multiple functional groups having similar property in the same matrix can improve its suitability as a promising candidate for oral peptide delivery with improved release characteristics, mucoadhesion as well as protecting the insulin activity and enhancing the permeability across the intestinal wall.
Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Name of substance:0 (Blood Glucose); 0 (Delayed-Action Preparations); 0 (Gastric Mucins); 0 (Insulin); 0 (Nanocapsules); 0 (Thiomalates); 5W494URQ81 (Streptozocin); 9012-76-4 (Chitosan)


  7 / 211 MEDLINE  
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PMID:25936377
Author:Brandt U; Deters A; Steinbüchel A
Address:Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität, Corrensstraße 3, D-48149, Münster, Germany.
Title:A jack-of-all-trades: 2-mercaptosuccinic acid.
Source:Appl Microbiol Biotechnol; 99(11):4545-57, 2015 Jun.
ISSN:1432-0614
Country of publication:Germany
Language:eng
Abstract:2-Mercaptosuccinic acid (MS) is an important and versatile substance for diverse fields of applications of which the most significant are surveyed in this article. Biological, chemical, and physical properties of MS as well as the knowledge of its synthesis and microbial degradation are illustrated. In addition, exemplary structural analogs of the organic sulfur compound are commented. The key application of MS in nanotechnology is discussed in detail with particular emphasis on quantum dots (nanocrystals) and self-assembled monolayers in combination with gold or silver. Furthermore, some medical and pharmaceutical applications are given, inter alia in bioimaging, as a nanocarrier, and with regard to the antimicrobial activity of MS-silver and MS-gold nanoparticles. Moreover, biological and chemical applications of MS are exemplified: the thiol compound can serve as an inhibitor for glutathione peroxidase, or the toxicity of substances can be increased due to the presence of MS in the respective cells or tissues. In the field of cosmetics, MS is widely utilized as a reducing agent for numerous products as explained in this article. Additionally, the microbial utilization of MS as a carbon and energy source for growth is elucidated in-depth, providing insight into different catabolic mechanisms.
Publication type:JOURNAL ARTICLE; REVIEW
Name of substance:0 (Thiomalates); 94239W5L4H (2-thiomalic acid)


  8 / 211 MEDLINE  
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PMID:25907598
Author:Schanuel FS; Raggio Santos KS; Monte-Alto-Costa A; de Oliveira MG
Address:Histology and Embryology Department, State University of Rio de Janeiro, UERJ, Rio de Janeiro 20950-003, Brazil.
Title:Combined nitric oxide-releasing poly(vinyl alcohol) film/F127 hydrogel for accelerating wound healing.
Source:Colloids Surf B Biointerfaces; 130:182-91, 2015 Jun 01.
ISSN:1873-4367
Country of publication:Netherlands
Language:eng
Abstract:Nitric oxide (NO) releasing biomaterials represent a potential strategy for use as active wound dressings capable of accelerating wound healing. Topical NO-releasing poly(vinyl alcohol) (PVA) films and Pluronic F127 hydrogels (F127) have already exhibited effective skin vasodilation and wound healing actions. In this study, we functionalized PVA films with SNO groups via esterification with a mixture of mercaptosucinic acid (MSA) and thiolactic acid (TLA) followed by S-nitrosation of the SH moieties. These films were combined with an underlying layer of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide), i.e., PEO-PPO-PEO (Pluronic F127) hydrogel and used for the topical treatment of skin lesions in an animal model. The mixed esterification of PVA with MSA and TLA led to chemically crosslinked PVA-SNO films with a high swelling capacity capable of spontaneously releasing NO. Real time NO-release measurements revealed that the hydrogel layer reduces the initial NO burst from the PVA-SNO films. We demonstrate that the combination of PVA-SNO films with F127 hydrogel accelerates wound contraction, decreases wound gap and cellular density and accelerates the inflammatory phase of the lesion. These results were reflected in an increase in myofibroblastic differentiation and collagen type III expression in the cicatricial tissue. Therefore, PVA-SNO films combined with F127 hydrogel may represent a new approach for active wound dressings capable of accelerating wound healing.
Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Name of substance:0 (Actins); 0 (Antigens, Differentiation); 0 (Hydrogels); 0 (PEO-PPO-PEO); 0 (Propylene Glycols); 0 (Sulfhydryl Compounds); 0 (Thiomalates); 0 (monocyte-macrophage differentiation antigen); 106392-12-5 (Poloxamer); 30IQX730WE (Polyethylene Glycols); 31C4KY9ESH (Nitric Oxide); 57564-91-7 (S-Nitrosoglutathione); 9002-89-5 (Polyvinyl Alcohol); 94239W5L4H (2-thiomalic acid); O5U6967KGF (thiolactic acid)


  9 / 211 MEDLINE  
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PMID:25609948
Author:Lin G; Wang X; Yin F; Yong KT
Address:The Engineering Lab of Synthetic Biology and Research Institute of Uropoiesis and Reproduction, School of Medicine, Shenzhen University, Shenzhen, People's Republic of China.
Title:Passive tumor targeting and imaging by using mercaptosuccinic acid-coated near-infrared quantum dots.
Source:Int J Nanomedicine; 10:335-45, 2015.
ISSN:1178-2013
Country of publication:New Zealand
Language:eng
Abstract:In this paper, we demonstrate the preparation of monodispersed quantum dots (QDs) as near-infrared (NIR) optical probes for in vivo pancreatic cancer targeting and imaging. The design of these luminescent probes involves functionalizing NIR QDs with ligand mercaptosuccinic acid (MSA), which targets the tumor site by enhanced permeability and retention effect. The colloidal and optical stability of the QDs can be maintained for >1 week. In vivo optical imaging studies in nude mice bearing pancreatic tumor show that the probes accumulate at tumor sites for >2.5 hours following intravenous injection of the functionalized NIR QDs. Tumor-labeling studies showed no evidence of harmful effects on the treated animals, even at a dose as high a ~50 mg/kg. These results demonstrate that the engineered MSA-functionalized QDs can serve as a diagnostic platform for early detection of cancer, as well as in image-guided precise surgical resection of tumors.
Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Name of substance:0 (Luminescent Agents); 0 (Thiomalates); 94239W5L4H (2-thiomalic acid)


  10 / 211 MEDLINE  
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PMID:25559489
Author:Tenório DP; Andrade CG; Cabral Filho PE; Sabino CP; Kato IT; Carvalho LB; Alves S; Ribeiro MS; Fontes A; Santos BS
Address:Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, PE, Brazil.
Title:CdTe quantum dots conjugated to concanavalin A as potential fluorescent molecular probes for saccharides detection in Candida albicans.
Source:J Photochem Photobiol B; 142:237-43, 2015 Jan.
ISSN:1873-2682
Country of publication:Switzerland
Language:eng
Abstract:Semiconductor colloidal quantum dots (QDs) have been applied in biological analysis due to their unique optical properties and their versatility to be conjugated to biomolecules, such as lectins and antibodies, acquiring specificity to label a variety of targets. Concanavalin A (Con A) lectin binds specifically to α-d-mannose and α-d-glucose regions of saccharides that are usually expressed on membranes of mammalian cells and on cell walls of microbials. Candida albicans is the most common fungal opportunistic pathogen present in humans. Therefore, in this work, this fungus was chosen as a model for understanding cells and biofilm-forming organisms. Here, we report an efficient bioconjugation process to bind CdTe (Cadmium Telluride) QDs to Con A, and applied the bioconjugates to label saccharide structures on the cellular surface of C. albicans suspensions and biofilms. By accomplishing hemagglutination experiments and circular dichroism, we observed that the Con A structure and biochemical properties were preserved after the bioconjugation. Fluorescence microscopy images of yeasts and hyphae cells, as well as biofilms, incubated with QDs-(Con A) showed a bright orange fluorescence profile, indicating that the cell walls were specifically labeled. Furthermore, flow cytometry measurements confirmed that over 93% of the yeast cells were successfully labeled by QD-(Con A) complex. In contrast, non-conjugated QDs or QDs-(inhibited Con A) do not label any kind of biological system tested, indicating that the bioconjugation was specific and efficient. The staining pattern of the cells and biofilms demonstrate that QDs were effectively bioconjugated to Con A with specific labeling of saccharide-rich structures on C. albicans. Consequently, this work opens new possibilities to monitor glucose and mannose molecules through fluorescence techniques, which can help to optimize phototherapy protocols for this kind of fungus.
Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
Name of substance:0 (Cadmium Compounds); 0 (Fluorescent Dyes); 0 (Thiomalates); 11028-71-0 (Concanavalin A); 94239W5L4H (2-thiomalic acid); IY9XDZ35W2 (Glucose); NQA0O090ZJ (Tellurium); PHA4727WTP (Mannose); STG188WO13 (cadmium telluride)



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