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PMID:29175396
Author:Wang F; Liu F; Chen W; Xu R; Wang W
Address:School of Biological Science, Luoyang Normal University, Luoyang, 471022, China; Cold Water Fish Breeding Engineering Technology Research Center of Henan Province, Luoyang, 471022, China. Electronic address: wangfan7677@163.com.
Title:Effects of triclosan (TCS) on hormonal balance and genes of hypothalamus-pituitary- gonad axis of juvenile male Yellow River carp (Cyprinus carpio).
Source:Chemosphere; 193:695-701, 2018 Feb.
ISSN:1879-1298
Country of publication:England
Language:eng
Abstract:Triclosan (TCS) is a broad spectrum antimicrobial agent which has been widely dispersed and determinated in the aquatic environment. However, the effects of TCS on reproductive endocrine in male fish are poorly understood. In this study, male Yellow River carp (Cyprinus carpio) were exposed to 0, 1/5, 1/10 and 1/20 LC (96 h LC of TCS to carp) TCS under semi-static conditions for 42 d. Vitellogenin (Vtg), 17ß-estradiol (E ), testosterone(T), gonadotropin (GtH), and gonadotropin-releasing hormone (GnRH) levels were measured by enzyme-linked immunosorbent assay (ELISA). Meanwhile, we also examined the mRNA expressions of aromatase, GtHs-ß, GnRH, estrogen receptor (Er), and androgen receptor (Ar) by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). TCS induced Vtg levels of hepatopancreas, E levels of serum, and inhibited Ar and Er mRNA levels, suggesting that the induction of Vtg production by TCS was indirectly caused by non-Er pathways. TCS-induced Vtg levels by interfering with the reproductive axis at plenty of latent loci of male carps: (a) TCS exposure increased the aromatase mRNA expression of hypothalamus and gonad aromatase, consequently increasing serum concentrations of E to induce Vtg in hepatopancreas; (b) TCS treatment changed GtH-ß and GnRH mRNA expression and secretion, causing the disturbance of reproductive endocrine; (c) TCS exposure decreased Ar mRNA levels, indicating potential Ar-mediated antiandrogen action. These mechanisms showed that TCS may induce Vtg production in male carp by non-Er-mediated pathways.
Publication type:JOURNAL ARTICLE
Name of substance:0 (Anti-Infective Agents); 0 (Hormones); 0 (RNA, Messenger); 0 (Receptors, Estrogen); 0 (Vitellogenins); 0 (Water Pollutants, Chemical); 33515-09-2 (Gonadotropin-Releasing Hormone); 3XMK78S47O (Testosterone); 4NM5039Y5X (Triclosan); 4TI98Z838E (Estradiol); EC 1.14.14.1 (Aromatase); EC 1.14.14.1 (CYP19A1 protein, human)


  2 / 3060 MEDLINE  
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PMID:28985654
Author:Tolussi CE; Gomes ADO; Kumar A; Ribeiro CS; Nostro FLL; Bain PA; de Souza GB; Cuña RD; Honji RM; Moreira RG
Address:Laboratório de Metabolismo e Reprodução de Organismos Aquáticos, Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo. Rua do Matão, Trav.14, n° 321, 05508-090 São Paulo, SP, Brazil. Electronic address: ctolussi@usp.br.
Title:Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae).
Source:Ecotoxicol Environ Saf; 147:926-934, 2018 Jan.
ISSN:1090-2414
Country of publication:Netherlands
Language:eng
Abstract:Endocrine disrupting compounds (EDCs) have the potential to alter fish reproduction at various levels of organization. The aim of this study was to assess the impact of a natural environment with heavily anthropogenic influence on the physiological processes involved in reproduction in the freshwater fish lambari (Astyanax fasciatus) using different biomarkers. Adult males and females were collected in different seasons from two distinct sites in the same watershed: Ponte Nova Reservoir (PN) considered a pristine or small anthropogenic influence reference point; and Billings Reservoir (Bil), subjected to a large anthropogenic impact. Biological indices, such as hepatosomatic index and gonadosomatic index (GSI), gonadal histomorphology, fecundity, and biomarkers such as plasma levels of estradiol (E2) as well as hepatic gene expression of its alfa nuclear receptor (ERα), were analyzed. Hepatic vitellogenin (VTG) gene expression was evaluated in both sexes, as an indicator of xenoestrogen exposure. Females collected at PN presented a typical annual variation reflected in GSI, whereas for those sampled at Bil the index did not change through the seasons. The higher concentration of E2 in males collected at Bil during spring/2013, together with the detection of VTG gene expression, suggest the presence of EDCs in the water. These EDCs may have also influenced fecundity of females from Bil, which was higher during winter and spring/2013. Gene expression of ERα and ovarian morphology did not differ between fish from both sites. Water conditions from Bil reservoir impacted by anthropic activity clearly interfered mainly with biomarkers of biological effect such as plasma E2 levels and absolute and relative fecundity, but also altered biomarkers of exposure as VTG gene expression. These facts support the notion that waterborne EDCs are capable of causing estrogenic activity in A. fasciatus.
Publication type:JOURNAL ARTICLE; REVIEW
Name of substance:0 (Biomarkers); 0 (Endocrine Disruptors); 0 (Vitellogenins); 0 (Water Pollutants, Chemical); 4TI98Z838E (Estradiol)


  3 / 3060 MEDLINE  
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PMID:29020644
Author:Pradhan A; Olsson PE; Jass J
Address:Biology, the Life Science Center, School of Science and Technology, Örebro University, SE-701 82 Örebro, Sweden. Electronic address: ajay.pradhan@oru.se.
Title:Di(2-ethylhexyl) phthalate and diethyl phthalate disrupt lipid metabolism, reduce fecundity and shortens lifespan of Caenorhabditis elegans.
Source:Chemosphere; 190:375-382, 2018 Jan.
ISSN:1879-1298
Country of publication:England
Language:eng
Abstract:The widespread use of phthalates is of major concern as they have adverse effects on many different physiological functions, including reproduction, metabolism and cell differentiation. The aim of this study was to compare the toxicity of the widely-used di (2-ethydlhexyl) phthalate (DEHP) with its substitute, diethyl phthalate (DEP). We analyzed the toxicity of these two phthalates using Caenorhabditis elegans as a model system. Gene expression analysis following exposure during the L1 to young adult stage showed that DEHP and DEP alter the expression of genes involved in lipid metabolism and stress response. Genes associated with lipid metabolism, including fasn-1, pod-2, fat-5, acs-6 and sbp-1, and vitellogenin were upregulated. Among the stress response genes, ced-1 wah-1, daf-21 and gst-4 were upregulated, while ctl-1, cdf-2 and the heat shock proteins (hsp-16.1, hsp-16.48 and sip-1) were downregulated. Lipid staining revealed that DEHP significantly increased lipid content following 1 µM exposure, however, DEP required 10 µM exposure to elicit an effect. Both DEHP and DEP reduced the fecundity at 1 µM concentration. Lifespan analysis indicated that DEHP and DEP reduced the average lifespan from 14 days in unexposed worms to 13 and 12 days, respectively. Expression of lifespan associated genes showed a correlation to shortened lifespan in the exposed groups. As reported previously, our data also indicates that the banned DEHP is toxic to C. elegans, however its substitute DEP has not been previously tested in this model organism and our data revealed that DEP is equally potent as DEHP in regulating C. elegans physiological functions.
Publication type:COMPARATIVE STUDY; JOURNAL ARTICLE
Name of substance:0 (Heat-Shock Proteins); 0 (Phthalic Acids); 0 (Vitellogenins); 6O7F7IX66E (phthalic acid); C42K0PH13C (Diethylhexyl Phthalate); UF064M00AF (diethyl phthalate)


  4 / 3060 MEDLINE  
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PMID:29112878
Author:Liu G; Ke M; Fan X; Zhang M; Zhu Y; Lu T; Sun L; Qian H
Address:College of Environment, Zhejiang University of Technology, Hangzhou, 310032, PR China; Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310032, PR China.
Title:Reproductive and endocrine-disrupting toxicity of Microcystis aeruginosa in female zebrafish.
Source:Chemosphere; 192:289-296, 2018 Feb.
ISSN:1879-1298
Country of publication:England
Language:eng
Abstract:Microcystis aeruginosa, a primary species in cyanobacterial blooms, is ubiquitously distributed in water. Microcystins (MCs) purified from M. aeruginosa can exert reproductive toxicity in fish. However, the effects of M. aeruginosa at environmentally relevant levels on the reproductive and endocrine systems of zebrafish are still unknown. The present study investigated the reproductive and endocrine-disrupting toxicity of M. aeruginosa on female zebrafish (Danio rerio) by short-term exposure (96 h). After exposure, marked histological lesions in the liver or gonads, such as nuclear pyknosis and deformation, were observed, and the fertilization rate and hatchability of eggs spawned from treated females were both significantly lower than they were in females in the control group, suggesting the possibility of transgenerational effects of M. aeruginosa exposure. Moreover, M. aeruginosa exposure decreased the concentration of 17ß-estradiol (E2) and testosterone (T) in female zebrafish. Interestingly, the vtg1 transcriptional level significantly decreased in the liver, whereas plasma vitellogenin (VTG) protein levels increased. The present findings indicate that M. aeruginosa could modulate endocrine function by disrupting transcription of hypothalamic-pituitary-gonadal-liver (HPGL) axis-related genes, and impair the reproductive capacity of female zebrafish, suggesting that M. aeruginosa causes potential adverse effects on fish reproduction in Microcystis bloom-contaminated aquatic environments.
Publication type:JOURNAL ARTICLE
Name of substance:0 (Bacterial Toxins); 0 (Endocrine Disruptors); 0 (Microcystins); 0 (Vitellogenins); 0 (Water Pollutants, Chemical); 0 (Zebrafish Proteins); 3XMK78S47O (Testosterone); 4TI98Z838E (Estradiol); 77238-39-2 (microcystin)


  5 / 3060 MEDLINE  
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PMID:28986080
Author:Kellock KA; Moore AP; Bringolf RB
Address:Warnell School of Forestry and Natural Resources, Interdisciplinary Toxicology Program, University of Georgia, 180 E. Green St., Athens, GA, USA.
Title:Chronic nitrate exposure alters reproductive physiology in fathead minnows.
Source:Environ Pollut; 232:322-328, 2018 Jan.
ISSN:1873-6424
Country of publication:England
Language:eng
Abstract:Nitrate is a ubiquitous aquatic pollutant that is commonly associated with eutrophication and dead zones in estuaries around the world. At high concentrations nitrate is toxic to aquatic life but at environmental concentrations it has also been purported as an endocrine disruptor in fish. To investigate the potential for nitrate to cause endocrine disruption in fish, we conducted a lifecycle study with fathead minnows (Pimephales promelas) exposed to nitrate (0, 11.3, and 56.5 mg/L (total nitrate-nitrogen (NO -N)) from <24 h post hatch to sexual maturity (209 days). Body mass, condition factor, gonadal somatic index (GSI), incidence of intersex, and vitellogenin induction were determined in mature male and female fish and plasma 11-keto testosterone (11-KT) was measured in males only. In nitrate-exposed males both 11-KT and vitellogenin were significantly induced when compared with controls. No significant differences occurred for body mass, condition factor, or GSI among males and intersex was not observed in any of the nitrate treatments. Nitrate-exposed females also had significant increases in vitellogenin compared to controls but no significant differences for mass, condition factor, or GSI were observed in nitrate exposed groups. Estradiol was used as a positive control for vitellogenin induction. Our findings suggest that environmentally relevant nitrate levels may disrupt steroid hormone synthesis and/or metabolism in male and female fish and may have implications for fish reproduction, watershed management, and regulation of nutrient pollution.
Publication type:JOURNAL ARTICLE
Name of substance:0 (Endocrine Disruptors); 0 (Nitrates); 0 (Vitellogenins); 0 (Water Pollutants, Chemical); 3XMK78S47O (Testosterone); 4TI98Z838E (Estradiol); KF38W1A85U (11-ketotestosterone)


  6 / 3060 MEDLINE  
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PMID:28862540
Author:Petersen K; Hultman MT; Bytingsvik J; Harju M; Evenset A; Tollefsen KE
Address:a Section of Ecotoxicology , Norwegian Institute for Water Research (NIVA) , Oslo , Norway.
Title:Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes.
Source:J Toxicol Environ Health A; 80(16-18):1017-1030, 2017.
ISSN:1528-7394
Country of publication:England
Language:eng
Abstract:Contaminants from various anthropogenic activities are detected in the Arctic due to long-range atmospheric transport, ocean currents, and living organisms such as migrating fish or seabirds. Although levels of persistent organic pollutants (POPs) in Arctic fish are generally low, local hot spots of contamination were found in freshwater systems such as Lake Ellasjøen at Bjørnøya (Bear Island, Norway). Higher concentrations of organic halogenated compounds (OHC), and higher levels of cytochrome P450 and DNA-double strand breaks were reported in Arctic char (Salvelinus alpinus) from this lake compared to fish from other lakes on Bjørnøya. Although several of the measured contaminants are potential endocrine disrupters, few studies have investigated potential endocrine disruptive effects of the contaminant cocktail in this fish population. The aim of this study was to compare acutely toxic and estrogenic potency of the cocktail of pollutants as evidenced by cytotoxic and/or estrogenic effects in vitro using extracts of Arctic char livers from contaminated Lake Ellasjøen with those from less contaminated Lake Laksvatn at Bjørnøya. This was performed by in situ sampling and contaminant extraction from liver tissue, followed by chemical analysis and in vitro testing of the following contaminated tissue extracts: F1-nonpolar OHC, F2-polar pesticides and metabolites of OHC, and F3-polar OHC. Contaminant levels were highest in extracts from Ellasjøen fish. The F2 and F3 extracts from Lake Laksvatn and Lake Ellasjøen fish reduced in vitro cell viability at a concentration ratio of 0.03-1 relative to tissue concentration in Arctic char. Only the F3 liver extract from Ellasjøen fish increased in vitro vitellogenin protein expression. Although compounds such as estrogenic OH-PCBs were quantified in Ellasjøen F3 extracts, it remains to be determined which compounds were inducing estrogenic effects.
Publication type:JOURNAL ARTICLE
Name of substance:0 (Endocrine Disruptors); 0 (Environmental Pollutants); 0 (Organic Chemicals); 0 (Tissue Extracts); 0 (Vitellogenins); 0 (Water Pollutants, Chemical)


  7 / 3060 MEDLINE  
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PMID:28849994
Author:Hultman MT; Petersen K; Tollefsen KE
Address:a Norwegian Institute for Water Research (NIVA) , Oslo , Norway.
Title:Characterizing combined effects of antiestrogenic chemicals on vitellogenin production in rainbow trout (Oncorhynchus mykiss) hepatocytes.
Source:J Toxicol Environ Health A; 80(16-18):987-1001, 2017.
ISSN:1528-7394
Country of publication:England
Language:eng
Abstract:Fish are exposed to a complex mixture of endocrine disrupting compounds (EDC), some of which display antiestrogenic activity leading to suppression of estrogen receptor (ER)- mediated reproductive processes. Although the main mode of action (MoA) of these antiestrogens is to directly interfere with natural ligand binding of the ER, several other MoA have been proposed. The aim of the present study was to characterize single and combined antiestrogenic effects of the aryl hydrocarbon receptor (AhR)-agonist ß-naphthoflavone (BNF) and ER-antagonist 4-hydroxytamoxifen (OHT) on vitellogenin (Vtg) protein using primary rainbow trout (Oncorhynchus mykiss) hepatocytes. Supporting transcriptional analysis of ER-responsive genes (estrogen receptor-α (er-α), vitellogenin-1 (vtg-1), eggshell zona radiata protein (zrp)) and AhR-mediated genes (aryl hydrocarbon receptor-2ß, cytochrome p450-1a (cyp1a)) was performed by qPCR to characterize the antiestrogenic influence on ER- and AhR-mediated responses. Data demonstrated that both BNF and OHT significantly reduced 17ß-estradiol (E2)-induced Vtg protein expression in a concentration responsive manner, whereas exposure to a mixture of these produced an additive antiestrogenic effect. The results observed at the protein level were further supported by transcriptional analysis of ER-responsive genes (er-α, vtg-1, zrp), where only E2-induced vtg-1 gene expression was significantly decreased by OHT and the mixture of OHT and BNF. E2-induced er-α and zrp gene expression was not markedly altered. The significant reduction of E2-induced vtg-1 gene expression by OHT suggested that the antiestrogenic effect of this compound may be associated with ER signaling pathway. Specific genes involved in putative AhR-ER cross-talk were also investigated, however none were directly associated with the compound anti-estrogenic MoA. Although the MoA of the single compounds and mixture were not completely characterized, the present study enhanced our knowledge of the combined toxicity mediated by antiestrogens acting through different MoA.
Publication type:JOURNAL ARTICLE
Name of substance:0 (Egg Proteins); 0 (Endocrine Disruptors); 0 (Estrogen Receptor Modulators); 0 (Estrogen Receptor alpha); 0 (Receptors, Aryl Hydrocarbon); 0 (Receptors, Estrogen); 0 (Vitellogenins); 0 (zona radiata protein, fish); 094ZI81Y45 (Tamoxifen); 17197F0KYM (afimoxifene); 4TI98Z838E (Estradiol); 6051-87-2 (beta-Naphthoflavone); EC 1.14.14.1 (Cytochrome P-450 CYP1A1)


  8 / 3060 MEDLINE  
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PMID:28753743
Author:Driessnack MK; Jamwal A; Niyogi S
Address:Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, Canada.
Title:Effects of chronic exposure to waterborne copper and nickel in binary mixture on tissue-specific metal accumulation and reproduction in fathead minnow (Pimephales promelas).
Source:Chemosphere; 185:964-974, 2017 Oct.
ISSN:1879-1298
Country of publication:England
Language:eng
Abstract:The current study evaluated the interactive effects of chronic waterborne copper (Cu) and nickel (Ni) exposure on tissue-specific metal accumulation and reproductive performance in fathead minnow (Pimephales promelas). Fish trios (1 male: 2 female; n = 5-6) were exposed for 21 days to: (i) control (no added Cu or Ni), (ii) waterborne Cu (45 µg/L), (iii) waterborne Ni (270 µg/L), and (iv) binary mixture of waterborne Cu and Ni (45 and 270 µg/L, respectively). Fish fecundity (cumulative egg production) was found to be the most sensitive reproductive endpoint, and the interaction of Cu and Ni elicited an additive effect on egg production. Tissue-specific accumulation of both metals was not influenced by the interaction of Cu and Ni, except an increased Cu and Ni burden in the carcass and ovary, respectively, were recorded. The expressions of hepatic estrogen receptor genes (ER-α and ER-ß) and the circulating estradiol level in females were also not affected by the metal-mixture treatment. However, co-exposure to waterborne Cu and Ni resulted in a significant downregulation of the hepatic vitellogenin gene in females, which was associated with the maximum upregulation of the hepatic metallothionein gene. In addition, a significant alteration of ovarian histopathology (decreased abundance of post-vitellogenic follicles, and increased follicular atresia) was also observed only in females exposed to Cu and Ni in mixture. Collectively, these observations suggest that chronic waterborne exposure to Cu and Ni in binary mixture may impair fish reproductive capacity by inducing histopathological damage in ovarian tissue, and disrupting of energy homeostasis in fish.
Publication type:JOURNAL ARTICLE
Name of substance:0 (Vitellogenins); 0 (Water Pollutants, Chemical); 789U1901C5 (Copper); 7OV03QG267 (Nickel); 9038-94-2 (Metallothionein)


  9 / 3060 MEDLINE  
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PMID:28722589
Author:Blariza MJ; Grosso CG; García BA
Address:Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Title:Silencing of Two Vitellogenin Genes Inhibits Oviposition in the Chagas Disease Vector (Hemiptera: Reduviidae).
Source:Am J Trop Med Hyg; 97(2):477-480, 2017 Aug.
ISSN:1476-1645
Country of publication:United States
Language:eng
Abstract:RNA interference caused by exogenous double-stranded RNA (dsRNA) is used to downregulate crucial genes to control insects. The reproductive success of all oviparous species depends on vitellogenin (Vg) biosynthesis and its accumulation in the developing oocytes. Adult females of were independently injected with two dsRNAs ( dsRNA or dsRNA) or nuclease-free water (control) 24 hours before feeding, and a group of adult females not injected was also analyzed (control). and messenger RNAs silencing was verified by reverse transcription quantitative polymerase chain reaction. The transcript levels of the and genes were significantly reduced after dsRNA treatment in fat body and ovary of in relation to those detected in individuals injected with nuclease-free water and not injected (controls). Moreover, the present study demonstrated that the silencing of the or genes inhibits oviposition in the Chagas disease vector . These findings may have important implications for the development of novel vector control strategies.
Publication type:COMPARATIVE STUDY; JOURNAL ARTICLE
Name of substance:0 (Vitellogenins)


  10 / 3060 MEDLINE  
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PMID:28690180
Author:Wang J; Wang J; Zhang Z; Zhang X; Ru S; Dong Y
Address:Marine Life Science College, Ocean University of China, Qingdao 266003, China.
Title:Development of an immunosensor for quantifying zebrafish vitellogenin based on the Octet system.
Source:Anal Biochem; 533:60-65, 2017 Sep 15.
ISSN:1096-0309
Country of publication:United States
Language:eng
Abstract:Vitellogenin (Vtg) is a sensitive biomarker for environmental estrogens. In this study, an immunosensor for quantifying zebrafish Vtg was developed using the Octet system. First, Protein A sensors were immobilized with purified anti-lipovitellin (Lv) antibody that demonstrated specificity to Vtg. Then, antibody-coated biosensors were immersed into zebrafish Lv standards and diluted samples. The Octet system measured and recorded kinetic parameters between antigens and captured antibody within 5 min. Sample Vtg concentrations were automatically calculated by interpolating relative binding rates observed with each sample and the immobilized anti-Lv antibody into the developed standard curve. The sensor arrays exhibited a wide linear range from 78 to 5000 ng/mL, and the inter-assay coefficient of variation was 0.66-1.97%. Furthermore, the performance of the immunosensor in detecting Vtg was evaluated by quantifying Vtg induction in juvenile zebrafish exposed to 17ß-estradiol (E ). Compared with conventional immunoassay techniques, the Vtg immunosensor developed based on the Octet system was much simpler and less time-consuming, allowing rapid Vtg quantification within 15 min. Moreover, Protein A sensors could be reused many times to ensure that the assays have high reproducibility. Therefore, we suggest that immunosensors based on the Octet system are an easily operated detection method for ecotoxicological research.
Publication type:JOURNAL ARTICLE
Name of substance:0 (Antibodies, Anti-Idiotypic); 0 (Egg Proteins); 0 (Staphylococcal Protein A); 0 (Vitellogenins); 4TI98Z838E (Estradiol); 9088-43-1 (lipovitellin)



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