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Pesquisa : A01.923.047.025.600.678 [Categoria DeCS]
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[PMID]:28468593
[Au] Autor:Ikemoto H; Lingasamy P; Anton Willmore AM; Hunt H; Kurm K; Tammik O; Scodeller P; Simón-Gracia L; Kotamraju VR; Lowy AM; Sugahara KN; Teesalu T
[Ad] Endereço:1 Laboratory of Cancer Biology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
[Ti] Título:Hyaluronan-binding peptide for targeting peritoneal carcinomatosis.
[So] Source:Tumour Biol;39(5):1010428317701628, 2017 May.
[Is] ISSN:1423-0380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Peritoneal carcinomatosis results from dissemination of solid tumors in the peritoneal cavity, and is a common site of metastasis in patients with carcinomas of gastrointestinal or gynecological origin. Peritoneal carcinomatosis treatment is challenging as poorly vascularized, disseminated peritoneal micro-tumors are shielded from systemic anticancer drugs and drive tumor regrowth. Here, we describe the identification and validation of a tumor homing peptide CKRDLSRRC (IP3), which upon intraperitoneal administration delivers payloads to peritoneal metastases. IP3 peptide was identified by in vivo phage display on a mouse model of peritoneal carcinomatosis of gastric origin (MKN-45P), using high-throughput sequencing of the peptide-encoding region of phage genome as a readout. The IP3 peptide contains a hyaluronan-binding motif, and fluorescein-labeled IP3 peptide bound to immobilized hyaluronan in vitro. After intraperitoneal administration in mice bearing peritoneal metastases of gastric and colon origin, IP3 peptide homed robustly to macrophage-rich regions in peritoneal tumors, including poorly vascularized micro-tumors. Finally, we show that IP3 functionalization conferred silver nanoparticles the ability to home to peritoneal tumors of gastric and colonic origin, suggesting that it could facilitate targeted delivery of nanoscale payloads to peritoneal tumors. Collectively, our study suggests that the IP3 peptide has potential applications for targeting drugs, nanoparticles, and imaging agents to peritoneal tumors.
[Mh] Termos MeSH primário: Carcinoma/tratamento farmacológico
Receptores de Hialuronatos/administração & dosagem
Peptídeos/administração & dosagem
Neoplasias Peritoneais/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Bacteriófagos/genética
Carcinoma/genética
Carcinoma/patologia
Linhagem Celular Tumoral
Modelos Animais de Doenças
Sistemas de Liberação de Medicamentos
Seres Humanos
Receptores de Hialuronatos/genética
Camundongos
Nanopartículas/administração & dosagem
Nanopartículas/química
Metástase Neoplásica
Peptídeos/genética
Cavidade Peritoneal/patologia
Neoplasias Peritoneais/genética
Neoplasias Peritoneais/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hyaluronan Receptors); 0 (Peptides)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1177/1010428317701628


  2 / 8914 MEDLINE  
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[PMID]:28455434
[Au] Autor:Izumi G; Koga K; Takamura M; Bo W; Nagai M; Miyashita M; Harada M; Hirata T; Hirota Y; Yoshino O; Fujii T; Osuga Y
[Ad] Endereço:Department of Obstetrics and Gynecology, The University of Tokyo 113-8655, Bunkyo, Tokyo, Japan.
[Ti] Título:Oil-Soluble Contrast Medium (OSCM) for Hysterosalpingography Modulates Dendritic Cell and Regulatory T Cell Profiles in the Peritoneal Cavity: A Possible Mechanism by Which OSCM Enhances Fertility.
[So] Source:J Immunol;198(11):4277-4284, 2017 06 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hysterosalpingography (HSG) with oil-soluble contrast medium (OSCM) is known to enhance fertility, although the mechanism is unclear. OSCM remains in the peritoneal cavity for several months after HSG. We hypothesized that OSCM that remains in the peritoneal cavity modulates dendritic cell (DC) and regulatory T cell (Treg) profiles and contributes to enhanced fertility. We characterized the profiles of DCs and Tregs in the peritoneal fluid from women who had undergone HSG. In vitro and in vivo effects of OSCM on monocyte-derived DCs and mouse peritoneal T cells were also evaluated. In comparison with women who have never experienced HSG, samples from women who had undergone HSG contained myeloid DCs with greater complexity and maturation, as well as had a marginally greater proportion of Tregs in their peritoneal fluid. OSCM is incorporated by monocyte-derived DCs, which causes their maturation and contributes to the increase in Treg proportions. Samples from OSCM-injected mice contained greater proportions of Tregs in comparison with controls. These studies demonstrate that OSCM modulates T cell profiles that are compatible with the condition observed in women who have undergone HSG. This study demonstrates that exogenous lipids administered to the peritoneal cavity are incorporated by DCs and that they significantly alter the immune environment in the peritoneal cavity. This immunological impact may contribute to enhanced fertility and the development of alternative therapeutic strategies for managing other pathological conditions associated with immunological abnormalities in the peritoneal cavity.
[Mh] Termos MeSH primário: Meios de Contraste/farmacologia
Células Dendríticas/imunologia
Fertilidade/imunologia
Histerossalpingografia
Cavidade Peritoneal/citologia
Linfócitos T Reguladores/imunologia
[Mh] Termos MeSH secundário: Adulto
Animais
Líquido Ascítico/citologia
Líquido Ascítico/imunologia
Meios de Contraste/administração & dosagem
Células Dendríticas/efeitos dos fármacos
Células Dendríticas/fisiologia
Feminino
Seres Humanos
Camundongos
Óleos
Solubilidade
Linfócitos T Reguladores/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Contrast Media); 0 (Oils)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180127
[Lr] Data última revisão:
180127
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1600498


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[PMID]:29245362
[Au] Autor:Kim YJ; Kim JS; Cho SH; Bae JI; Sohn CH; Lee YS; Lee JH; Lim KS; Kim WY
[Ad] Endereço:Department of Emergency Medicine, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
[Ti] Título:Characteristics of computed tomography in hemodynamically unstable blunt trauma patients: Experience at a tertiary care center.
[So] Source:Medicine (Baltimore);96(49):e9168, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Emergent exploratory laparotomy is recommended for hemodynamically unstable blunt trauma patients suspected of having hemoperitoneum. However, given the unreliability of ultrasonography and rapid scan speed of computed tomography (CT), CT might help clinicians provide accurate information even in hemodynamically unstable trauma patients. This observational study aimed to describe the bleeding site and hospital course of severe blunt trauma patients with hemoperitoneum diagnosed by CT scan.We enrolled all consecutive adult blunt trauma patients (≥18 years old) who underwent whole-body CT before operation between February 2012 and October 2016. Patients with hemoperitoneum on CT images were included and categorized into hemodynamically stable and unstable (persistent hypotension despite fluid resuscitation) groups.Among 1723 severe blunt trauma patients, 136 patients with hemoperitoneum were included. Of these, 98 (72.1%) patients had documented intraperitoneal injury, and the liver (60.2%) was most frequently damaged site, followed by spleen (23.5%) and mesentery (23.5%). The rate of intraperitoneal organ injury did not differ between hemodynamically stable (n = 107) and unstable (n = 29) groups (69.2% vs 82.8%, P = .15), while the documented active internal bleeding was high in the unstable group (29.9% vs 69.0%, P < .001). In the unstable group, 14 (48.3%) patients underwent emergent operation, while 3 patients underwent embolization, and the others were treated in a conservative manner.Even in hemodynamically unstable hemoperitoneum patients, 17.2% had no documented intraperitoneal injury and over half of the patients were treated without emergent operation.
[Mh] Termos MeSH primário: Hemorragia/diagnóstico por imagem
Hemorragia/etiologia
Cavidade Peritoneal/lesões
Ferimentos não Penetrantes/complicações
Ferimentos não Penetrantes/diagnóstico por imagem
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
Fígado/lesões
Masculino
Mesentério/lesões
Meia-Idade
Baço/lesões
Centros de Atenção Terciária
Tomografia Computadorizada por Raios X/métodos
Índices de Gravidade do Trauma
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009168


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[PMID]:28922373
[Au] Autor:King A; Li L; Wong DM; Liu R; Bamford R; Strasser A; Tarlinton DM; Heierhorst J
[Ad] Endereço:Molecular Genetics Unit, St. Vincent's Institute of Medical Research, Fitzroy, Victoria, Australia.
[Ti] Título:Dynein light chain regulates adaptive and innate B cell development by distinctive genetic mechanisms.
[So] Source:PLoS Genet;13(9):e1007010, 2017 Sep.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mechanistic differences in the development and function of adaptive, high-affinity antibody-producing B-2 cells and innate-like, "natural" antibody-producing B-1a cells remain poorly understood. Here we show that the multi-functional dynein light chain (DYNLL1/LC8) plays important roles in the establishment of B-1a cells in the peritoneal cavity and in the ongoing development of B-2 lymphoid cells in the bone marrow of mice. Epistasis analyses indicate that Dynll1 regulates B-1a and early B-2 cell development in a single, linear pathway with its direct transcriptional activator ASCIZ (ATMIN/ZNF822), and that the two genes also have complementary functions during late B-2 cell development. The B-2 cell defects caused by loss of DYNLL1 were associated with lower levels of the anti-apoptotic protein BCL-2, and could be supressed by deletion of pro-apoptotic BIM which is negatively regulated by both DYNLL1 and BCL-2. Defects in B cell development caused by loss of DYNLL1 could also be partially suppressed by a pre-arranged SWHEL Igm-B cell receptor transgene. In contrast to the rescue of B-2 cell numbers, the B-1a cell deficiency in Dynll1-deleted mice could not be suppressed by the loss of Bim, and was further compounded by the SWHEL transgene. Conversely, oncogenic MYC expression, which is synthetic lethal with Dynll1 deletion in B-2 cells, did not further reduce B-1a cell numbers in Dynll1-defcient mice. Finally, we found that the ASCIZ-DYNLL1 axis was also required for the early-juvenile development of aggressive MYC-driven and p53-deficient B cell lymphomas. These results identify ASCIZ and DYNLL1 as the core of a transcriptional circuit that differentially regulates the development of the B-1a and B-2 B lymphoid cell lineages and plays a critical role in lymphomagenesis.
[Mh] Termos MeSH primário: Linfócitos B/metabolismo
Dineínas/genética
Linfoma de Células B/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Animais
Linfócitos B/imunologia
Linhagem da Célula/genética
Dineínas/metabolismo
Regulação da Expressão Gênica
Seres Humanos
Linfócitos/metabolismo
Linfócitos/patologia
Linfoma de Células B/patologia
Camundongos
Cavidade Peritoneal
Proteínas Proto-Oncogênicas c-myc/genética
Fatores de Transcrição/metabolismo
Proteína Supressora de Tumor p53/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATMIN protein, mouse); 0 (Proto-Oncogene Proteins c-myc); 0 (Transcription Factors); 0 (Tumor Suppressor Protein p53); EC 3.6.4.2 (DYNLL1 protein, mouse); EC 3.6.4.2 (Dyneins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007010


  5 / 8914 MEDLINE  
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[PMID]:28739829
[Au] Autor:Witola WH; Kim CY; Zhang X
[Ad] Endereço:Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA whwit35@illinois.edu.
[Ti] Título:Inherent Oxidative Stress in the Lewis Rat Is Associated with Resistance to Toxoplasmosis.
[So] Source:Infect Immun;85(10), 2017 Oct.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The course of infection in rats closely resembles that in humans. However, compared to the Brown Norway (BN) rat, the Lewis (LEW) rat is extremely resistant to infection. Thus, we performed RNA sequencing analysis of the LEW rat versus the BN rat, with or without infection, in order to unravel molecular factors directing robust and rapid early -killing mechanisms in the LEW rat. We found that compared to the uninfected BN rat, the uninfected LEW rat has inherently higher transcript levels of cytochrome enzymes (Cyp2d3, Cyp2d5, and Cybrd1, which catalyze generation of reactive oxygen species [ROS]), with concomitant higher levels of ROS. Interestingly, despite having higher levels of ROS, the LEW rat had lower transcript levels for antioxidant enzymes (lactoperoxidase, microsomal glutathione -transferase 2 and 3, glutathione -transferase peroxidase kappa 1, and glutathione peroxidase) than the BN rat, suggesting that the LEW rat maintains cellular oxidative stress that it tolerates. Corroboratively, we found that scavenging of superoxide anion by Mn(III) tetrakis (4-benzoic acid) porphyrin (MnTBAP) decreased the refractoriness of LEW rat peritoneal cells to infection, resulting in proliferation of parasites in LEW rat peritoneal cells which, in turn, led to augmented cell death in the infected cells. Together, our results indicate that the LEW rat maintains inherent cellular oxidative stress that contributes to resistance to invading , and they thus unveil new avenues for developing therapeutic agents targeting induction of host cell oxidative stress as a mechanism for killing .
[Mh] Termos MeSH primário: Resistência à Doença
Estresse Oxidativo
Toxoplasmose Animal/imunologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Morte Celular
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Citocromos/genética
Glutationa Peroxidase/genética
Glutationa Peroxidase/metabolismo
Glutationa Transferase/genética
Glutationa Transferase/metabolismo
Lactoperoxidase/genética
Lactoperoxidase/metabolismo
Cavidade Peritoneal/parasitologia
Ratos
Ratos Endogâmicos BN
Ratos Endogâmicos Lew
Espécies Reativas de Oxigênio/metabolismo
Análise de Sequência de RNA/métodos
Toxoplasma/imunologia
Toxoplasma/fisiologia
Toxoplasmose Animal/metabolismo
Toxoplasmose Animal/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Cytochromes); 0 (Reactive Oxygen Species); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.- (Cyp2d3 protein, rat); EC 1.11.1.- (Lactoperoxidase); EC 1.11.1.9 (Glutathione Peroxidase); EC 2.5.1.18 (Glutathione Transferase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


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[PMID]:28704709
[Au] Autor:Meng X; Sun W; Ren Y; Xiao Y; Zhao P; Lu W; Hua L; Wang L; Wang L; Yu Y
[Ad] Endereço:Department of Immunology, College of Basic Medical Sciences, Norman Bethune Health Science Center, Jilin University, Changchun, Jilin, China; Department of Endodontics, School and Hospital of Stomatology, Jilin University, Changchun, Jilin, China.
[Ti] Título:Protective role of surface Toll-like receptor 9 expressing neutrophils in local inflammation during systemic inflammatory response syndrome in mice.
[So] Source:Mol Immunol;90:74-86, 2017 Oct.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Clinically, systemic inflammatory response syndrome (SIRS) occurs after serious trauma or sepsis. In sepsis, neutrophils are the major effector cells responsible for eliminating pathogens. However, the role of neutrophils in development of SIRS, especially in local inflammatory area, is controversial. In this study, we established a SIRS mouse model characterized with cytokine-mediated lethal shock by intraperitoneal injection of oligodexynucleotides containing CpG motifs (CpG ODN) in D-galactosamine (D-GalN) sensitized mice based on our previous work and found that abundant neutrophils were rapidly recruited into the peritoneal cavity, where some neutrophils expressed surface TLR9 (sTLR9), defined as sTLR9 neutrophils. Along with the progression of SIRS, the expression of sTLR9 in sTLR9 neutrophils isolated from peritoneal lavage cells (PLCs) was declined in accompany with an increase in the level of the inflammatory cytokine TNFα and a decrease in the level of the anti-inflammatory cytokine IL-10 in Ly6G PLCs. When using CCT ODN, an oligodeoxynucleotide with CCT repeats, which we have previously shown to be capable of rescuing mice from lethal shock, the expression of sTLR9 on neutrophils was significantly elevated. Adoptive therapy using early recruited neutrophil-rich PLCs containing sTLR9 neutrophils that express high levels of sTLR9, could rescue mice from SIRS. Overall, the data reveal that the early recruited sTLR9 neutrophils may, at least in the area of local inflammation, play a protective role during SIRS development and provide a method to rescue the patients with severe SIRS via the up-regulation of sTLR9 levels on the surface of neutrophils or via adoptive therapy with protective sub-populations of neutrophils.
[Mh] Termos MeSH primário: Transferência Adotiva/métodos
Neutrófilos/imunologia
Cavidade Peritoneal/citologia
Síndrome de Resposta Inflamatória Sistêmica/patologia
Receptor Toll-Like 9/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Feminino
Inflamação/imunologia
Interleucina-10/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Neutrófilos/transplante
Oligonucleotídeos
Cavidade Peritoneal/patologia
Síndrome de Resposta Inflamatória Sistêmica/imunologia
Receptor Toll-Like 9/biossíntese
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (IL10 protein, mouse); 0 (Oligonucleotides); 0 (Tlr9 protein, mouse); 0 (Toll-Like Receptor 9); 0 (Tumor Necrosis Factor-alpha); 130068-27-8 (Interleukin-10)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE


  7 / 8914 MEDLINE  
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[PMID]:28682921
[Au] Autor:Tanaka T; Terai Y; Maeda K; Ashihara K; Kogata Y; Maruoka H; Terada S; Yamada T; Ohmichi M
[Ad] Endereço:aDepartment of Obstetrics and Gynecology bDepartment of Pathology, Osaka Medical College, Takatsuki, Osaka, Japan.
[Ti] Título:Intraperitoneal cytology after laparoscopic hysterectomy in patients with endometrial cancer: A retrospective observational study.
[So] Source:Medicine (Baltimore);96(27):e7502, 2017 Jul.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate the dissemination of cancer cells at laparoscopic hysterectomy according to the intraperitoneal cytology.Patients with endometrial cancer underwent total laparoscopic modified radical hysterectomy. Peritoneal wash cytology was performed on entering the peritoneal cavity before surgical preparation and just after hysterectomy.Seventy-eight patients underwent laparoscopic hysterectomy for endometrial cancer. Among the 15 patients who had positive intraperitoneal cytology on entering the peritoneal cavity, 10 converted to negative intraperitoneal cytology after hysterectomy. In contrast, among the 63 patients who had negative intraperitoneal cytology on entering the peritoneal cavity, 2 converted to positive intraperitoneal cytology after hysterectomy.While surgery can reduce the number of cancer cells in the peritoneal cavity, leakage can occur, as seen in some cases of hysterectomy. Careful washing must be performed after hysterectomy.
[Mh] Termos MeSH primário: Neoplasias do Endométrio/cirurgia
Histerectomia
Laparoscopia
Cavidade Peritoneal/citologia
Lavagem Peritoneal
[Mh] Termos MeSH secundário: Índice de Massa Corporal
Neoplasias do Endométrio/patologia
Feminino
Seres Humanos
Meia-Idade
Gradação de Tumores
Inoculação de Neoplasia
Estadiamento de Neoplasias
Cavidade Peritoneal/patologia
Estudos Retrospectivos
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000007502


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[PMID]:28545460
[Au] Autor:Horning M; Haulena M; Rosenberg JF; Nordstrom C
[Ad] Endereço:Alaska SeaLife Center, 301 Railway Avenue, Seward, AK, 99664, USA. markush@alaskasealife.org.
[Ti] Título:Intraperitoneal implantation of life-long telemetry transmitters in three rehabilitated harbor seal pups.
[So] Source:BMC Vet Res;13(1):139, 2017 May 25.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Pinnipeds, including many phocid species of concern, are inaccessible and difficult to monitor for extended periods using conventional, externally attached telemetry devices that are shed during the annual molt. Archival satellite transmitters were implanted intraperitoneally into three stranded Pacific harbor seal pups (Phoca vitulina richardii) that completed rehabilitation, to evaluate the viability of this surgical technique for the deployment of life long telemetry devices in phocids. The life history transmitters record information throughout the life of the host and transmit data to orbiting satellites after extrusion following death. RESULTS: Surgeries were performed under general anesthesia and a single transmitter was inserted into the ventrocaudal abdominal cavity via a 7-8 cm incision along the ventral midline between the umbilicus and pubic symphysis or preputial opening in each animal. Surgeries lasted from 45 to 51 min, and anesthesic times ranged from 55 to 79 min. All animals recovered well, were released into dry holding pens overnight, and were given access to water the following day. All three animals exhibited an expected inflammatory response, with acute phase responses lasting approximately three to four weeks. All three animals were tracked via externally attached satellite transmitters after release at 58 to 78 days following surgery, and minimum post-release survival was confirmed through continued movement data received over 278 to 289 days. CONCLUSION: The initial findings of low morbidity and zero mortality encountered during captive observation and post-release tracking periods support the viability of this surgical technique for the implantation of long-term telemetry devices in phocids.
[Mh] Termos MeSH primário: Cavidade Peritoneal/cirurgia
Phoca/cirurgia
Próteses e Implantes/veterinária
Telemetria/veterinária
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Temperatura Corporal
Feminino
Cuidados Pós-Operatórios/veterinária
Telemetria/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-017-1060-1


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[PMID]:28542390
[Au] Autor:McGuire AL; Mulroney KT; Carson CF; Ram R; Morahan G; Chakera A
[Ad] Endereço:Translational Renal Research Group, Harry Perkins Institute of Medical Research, Nedlands, Western Australia, Australia.
[Ti] Título:Analysis of early mesothelial cell responses to Staphylococcus epidermidis isolated from patients with peritoneal dialysis-associated peritonitis.
[So] Source:PLoS One;12(5):e0178151, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The major complication of peritoneal dialysis (PD) is the development of peritonitis, an infection within the abdominal cavity, primarily caused by bacteria. PD peritonitis is associated with significant morbidity, mortality and health care costs. Staphylococcus epidermidis is the most frequently isolated cause of PD-associated peritonitis. Mesothelial cells are integral to the host response to peritonitis, and subsequent clinical outcomes, yet the effects of infection on mesothelial cells are not well characterised. We systematically investigated the early mesothelial cell response to clinical and reference isolates of S. epidermidis using primary mesothelial cells and the mesothelial cell line Met-5A. Using an unbiased whole genome microarray, followed by a targeted panel of genes known to be involved in the human antibacterial response, we identified 38 differentially regulated genes (adj. p-value < 0.05) representing 35 canonical pathways after 1 hour exposure to S. epidermidis. The top 3 canonical pathways were TNFR2 signaling, IL-17A signaling, and TNFR1 signaling (adj. p-values of 0.0012, 0.0012 and 0.0019, respectively). Subsequent qPCR validation confirmed significant differences in gene expression in a number of genes not previously described in mesothelial cell responses to infection, with heterogeneity observed between clinical isolates of S. epidermidis, and between Met-5A and primary mesothelial cells. Heterogeneity between different S. epidermidis isolates suggests that specific virulence factors may play critical roles in influencing outcomes from peritonitis. This study provides new insights into early mesothelial cell responses to infection with S. epidermidis, and confirms the importance of validating findings in primary mesothelial cells.
[Mh] Termos MeSH primário: Diálise Peritoneal/efeitos adversos
Peritonite/etiologia
Peritonite/microbiologia
Infecções Estafilocócicas/etiologia
Infecções Estafilocócicas/microbiologia
Staphylococcus epidermidis/patogenicidade
[Mh] Termos MeSH secundário: Linhagem Celular
Células Cultivadas
Células Epiteliais/imunologia
Células Epiteliais/microbiologia
Expressão Gênica
Interações Hospedeiro-Patógeno/genética
Interações Hospedeiro-Patógeno/imunologia
Seres Humanos
Interleucina-17/genética
Cavidade Peritoneal/microbiologia
Peritonite/genética
Receptores Tipo I de Fatores de Necrose Tumoral/genética
Receptores Tipo II do Fator de Necrose Tumoral/genética
Transdução de Sinais/genética
Transdução de Sinais/imunologia
Infecções Estafilocócicas/genética
Staphylococcus epidermidis/isolamento & purificação
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (IL17A protein, human); 0 (Interleukin-17); 0 (Receptors, Tumor Necrosis Factor, Type I); 0 (Receptors, Tumor Necrosis Factor, Type II)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178151


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[PMID]:28483060
[Au] Autor:Rebibo L; Ebosse I; Iederan C; Mahjoub Y; Dupont H; Cosse C; Regimbeau JM
[Ad] Endereço:Department of Digestive Surgery, Amiens University Hospital, Amiens, France.
[Ti] Título:Does drainage of the peritoneal cavity have an impact on the postoperative course of community-acquired, secondary, lower gastrointestinal tract peritonitis?
[So] Source:Am J Surg;214(1):29-36, 2017 Jul.
[Is] ISSN:1879-1883
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In the surgical management of lower gastrointestinal tract peritonitis (LGTP), drainage of the peritoneal cavity is often recommended. The objective of the study was to evaluate the impact of drainage of the abdominal cavity during management of LGTP. METHODS: From January 2009 to January 2012, patients undergoing surgery for LGTP were included. The study comprised 3 steps: (1) description of the overall population; (2) comparison of the "no drainage" and "drainage" groups; and (3) a propensity score-matched analysis. The primary end point was the major complications rate; secondary end points were the overall complication, risk factors for postoperative complications, and the length of hospital stay. RESULTS: A total of 205 patients underwent surgery for LGTP. Characteristics of the peritoneum were noted on the surgical report in 141 cases (68%). Abdominal drainage was implemented in 118 patients (83%). After propensity score matching, there was no difference between drainage and no drainage groups in the major postoperative complications (34.7% vs 34.8%; P = .89). CONCLUSIONS: Drainage of the abdominal cavity had no impact on postoperative abscess and reoperation rates. Standardization of drainage in this context is required.
[Mh] Termos MeSH primário: Drenagem/utilização
Cavidade Peritoneal
Peritonite/terapia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Infecções Comunitárias Adquiridas/epidemiologia
Feminino
França/epidemiologia
Seres Humanos
Tempo de Internação/estatística & dados numéricos
Masculino
Meia-Idade
Peritonite/epidemiologia
Complicações Pós-Operatórias
Padrões de Prática Médica
Pontuação de Propensão
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE



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