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[PMID]:28463576
[Au] Autor:Li K; Zhang C; Qiu L; Gao L; Zhang X
[Ad] Endereço:Tianjin Key Laboratory of Design and Intelligent Control of the Advanced Mechatronical System, School of Mechanical Engineering, Tianjin University of Technology , Tianjin, China .
[Ti] Título:Advances in Application of Mechanical Stimuli in Bioreactors for Cartilage Tissue Engineering.
[So] Source:Tissue Eng Part B Rev;23(4):399-411, 2017 Aug.
[Is] ISSN:1937-3376
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Articular cartilage (AC) is the weight-bearing tissue in diarthroses. It lacks the capacity for self-healing once there are injuries or diseases due to its avascularity. With the development of tissue engineering, repairing cartilage defects through transplantation of engineered cartilage that closely matches properties of native cartilage has become a new option for curing cartilage diseases. The main hurdle for clinical application of engineered cartilage is how to develop functional cartilage constructs for mass production in a credible way. Recently, impressive hyaline cartilage that may have the potential to provide capabilities for treating large cartilage lesions in the future has been produced in laboratories. The key to functional cartilage construction in vitro is to identify appropriate mechanical stimuli. First, they should ensure the function of metabolism because mechanical stimuli play the role of blood vessels in the metabolism of AC, for example, acquiring nutrition and removing wastes. Second, they should mimic the movement of synovial joints and produce phenotypically correct tissues to achieve the adaptive development between the micro- and macrostructure and function. In this article, we divide mechanical stimuli into three types according to forces transmitted by different media in bioreactors, namely forces transmitted through the liquid medium, solid medium, or other media, then we review and summarize the research status of bioreactors for cartilage tissue engineering (CTE), mainly focusing on the effects of diverse mechanical stimuli on engineered cartilage. Based on current researches, there are several motion patterns in knee joints; but compression, tension, shear, fluid shear, or hydrostatic pressure each only partially reflects the mechanical condition in vivo. In this study, we propose that rolling-sliding-compression load consists of various stimuli that will represent better mechanical environment in CTE. In addition, engineers often ignore the importance of biochemical factors to the growth and development of engineered cartilage. In our point of view, only by fully considering synergistic effects of mechanical and biochemical factors can we find appropriate culture conditions for functional cartilage constructs. Once again, rolling-sliding-compression load under appropriate biochemical conditions may be conductive to realize the adaptive development between the structure and function of engineered cartilage in vitro.
[Mh] Termos MeSH primário: Reatores Biológicos
[Mh] Termos MeSH secundário: Cartilagem
Cartilagem Articular
Condrócitos
Estresse Mecânico
Engenharia Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1089/ten.TEB.2016.0427


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[PMID]:29367523
[Au] Autor:Umeda N; Matsumoto I; Sumida T
[Ad] Endereço:Department of Rheumatology, Tsuchiura Kyodo General Hospital.
[Ti] Título:[The pathogenic role of ACPA in rheumatoid arthritis].
[So] Source:Nihon Rinsho Meneki Gakkai Kaishi;40(6):391-395, 2017.
[Is] ISSN:1349-7413
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:  In rheumatoid arthritis (RA), ACPA (anti-citrullinated protein/peptide antibody) is elevated with high specificity, and clinically, anti-CCP (cyclic citrullinated peptide) antibody is widely used for diagnosis of RA. It is thought ACPAs are produced with genetic background such as HLA-DR, environmental factors such as periodontal disease and smoking, however, the pathogenic role of ACPA in RA has not been elucidated. These were showed immune complexes including ACPA or ACPA itself promoted inflammatory cytokine production such as TNF. PADs (peptidylarginine deiminases) were expressed and citrullinated proteins existed in RA synovium. ACPAs were deposited on the site of citrulline in CD68 positive cells of RA synovium. The damage of bone and cartilage is observed in RA. It was also suggested that deposition of ACPAs caused osteoclastogenesis and bone loss. We introduce several findings about the pathogenic role of ACPA in RA.
[Mh] Termos MeSH primário: Anticorpos Anti-Proteína Citrulinada/efeitos adversos
Anticorpos Anti-Proteína Citrulinada/imunologia
Artrite Reumatoide/imunologia
[Mh] Termos MeSH secundário: Antígenos CD
Antígenos de Diferenciação Mielomonocítica
Artrite Reumatoide/diagnóstico
Artrite Reumatoide/metabolismo
Artrite Reumatoide/patologia
Reabsorção Óssea/imunologia
Osso e Ossos/patologia
Cartilagem/patologia
Citrulina/imunologia
Citrulina/metabolismo
Antígenos HLA-DR
Seres Humanos
Mediadores da Inflamação/metabolismo
Osteogênese/imunologia
Doenças Periodontais
Desiminases de Arginina em Proteínas/metabolismo
Fumar
Membrana Sinovial/citologia
Membrana Sinovial/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Citrullinated Protein Antibodies); 0 (Antigens, CD); 0 (Antigens, Differentiation, Myelomonocytic); 0 (CD68 antigen, human); 0 (HLA-DR Antigens); 0 (Inflammation Mediators); 0 (Tumor Necrosis Factor-alpha); 29VT07BGDA (Citrulline); EC 3.5.3.15 (Protein-Arginine Deiminases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.2177/jsci.40.391


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[PMID]:28457885
[Au] Autor:Brunger JM; Zutshi A; Willard VP; Gersbach CA; Guilak F
[Ad] Endereço:Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
[Ti] Título:Genome Engineering of Stem Cells for Autonomously Regulated, Closed-Loop Delivery of Biologic Drugs.
[So] Source:Stem Cell Reports;8(5):1202-1213, 2017 May 09.
[Is] ISSN:2213-6711
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic inflammatory diseases such as arthritis are characterized by dysregulated responses to pro-inflammatory cytokines such as interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α). Pharmacologic anti-cytokine therapies are often effective at diminishing this inflammatory response but have significant side effects and are used at high, constant doses that do not reflect the dynamic nature of disease activity. Using the CRISPR/Cas9 genome-engineering system, we created stem cells that antagonize IL-1- or TNF-α-mediated inflammation in an autoregulated, feedback-controlled manner. Our results show that genome engineering can be used successfully to rewire endogenous cell circuits to allow for prescribed input/output relationships between inflammatory mediators and their antagonists, providing a foundation for cell-based drug delivery or cell-based vaccines via a rapidly responsive, autoregulated system. The customization of intrinsic cellular signaling pathways in stem cells, as demonstrated here, opens innovative possibilities for safer and more effective therapeutic approaches for a wide variety of diseases.
[Mh] Termos MeSH primário: Edição de Genes/métodos
Fatores Imunológicos/genética
Células-Tronco Pluripotentes Induzidas/metabolismo
Transplante de Células-Tronco/métodos
[Mh] Termos MeSH secundário: Animais
Artrite/terapia
Sistemas CRISPR-Cas
Cartilagem/fisiologia
Células Cultivadas
Retroalimentação Fisiológica
Fatores Imunológicos/metabolismo
Células-Tronco Pluripotentes Induzidas/citologia
Células-Tronco Pluripotentes Induzidas/transplante
Interleucina-1/genética
Interleucina-1/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Regeneração
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunologic Factors); 0 (Interleukin-1); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:27771937
[Au] Autor:Huang YC; Xiao J; Leung WY; Lu WW; Hu Y; Luk KD
[Ad] Endereço:Department of Orthopaedics and Traumatology, The University of Hong Kong, 5/F Professor Block, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China.hrmoldk@hku.hk.
[Ti] Título:Lumbar intervertebral disc allograft transplantation: healing and remodelling of the bony structure.
[So] Source:Eur Cell Mater;32:216-227, 2016 10 19.
[Is] ISSN:1473-2262
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:Previous human study suggested that fresh-frozen intervertebral disc allograft transplantation can relieve neurological symptoms and restore segmental kinematics. Before wide clinical application, research into the pathophysiology of the postoperative disc allograft is needed. One important question that remains to be answered in disc allografting is the healing process of the host-graft interface and the subsequent change of the endplates. With the goat model for lumbar disc allografting, histology, micro-computed tomography analysis, scanning electron microscopy and energy-dispersive X-ray spectroscopy mapping were applied to evaluate the healing of the host-graft interfaces, the remodelling of subchondral bone, and the changes of the bony and cartilaginous endplates after transplantation. It was found that healing of the host-graft interfaces started at 1.5 months and was completed at 6 months by natural remodelling. This bony remodelling was also noted in the subchondral bone area after 6 months. The bony endplate was well preserved initially, but was gradually replaced by trabecular bone afterwards; on the other hand, the cartilaginous endplate became atrophic at 6 months and nearly disappeared at the final follow-up. Collectively, after intervertebral disc allograft transplantation, bony healing and remodelling were seen which ensured the stability and mobility of the disc-transplanted segment, but the integrity of bony and cartilaginous endplates was gradually lost and nearly disappeared finally.
[Mh] Termos MeSH primário: Aloenxertos/transplante
Disco Intervertebral/transplante
Vértebras Lombares/transplante
Cicatrização
[Mh] Termos MeSH secundário: Animais
Cartilagem/diagnóstico por imagem
Cartilagem/patologia
Cabras
Disco Intervertebral/diagnóstico por imagem
Disco Intervertebral/ultraestrutura
Vértebras Lombares/diagnóstico por imagem
Vértebras Lombares/ultraestrutura
Masculino
Espectrometria por Raios X
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:29252744
[Au] Autor:Zimmerman RM; Allen EA; Higgins JP
[Ad] Endereço:The Curtis National Hand Center, MedStar Union Memorial Hospital, Baltimore, Maryland.
[Ti] Título:Chondrocyte Viability 3.5 Years Following a Vascularized Medial Femoral Trochlea Osteocartilaginous Free Flap.
[So] Source:JBJS Case Connect;6(4):e90, 2016 Oct-Dec.
[Is] ISSN:2160-3251
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:CASE: A 30-year-old woman underwent arthroscopy for wrist pain 3.5 years following a medial femoral trochlea (MFT) osteocartilaginous free flap for Kienböck disease, which provided the opportunity to examine the transferred cartilage in vivo. Arthroscopy revealed no evidence of chondrolysis, and histologic examination revealed uniformly viable chondrocytes within a matrix consistent with fibrocartilage. CONCLUSION: To the best of our knowledge, the long-term viability of chondrocytes following an MFT free flap has not been previously documented. This case provides early evidence that free tissue transfer based on the descending genicular artery can provide a durable solution for osteocartilaginous defects.
[Mh] Termos MeSH primário: Cartilagem/fisiologia
Condrócitos/fisiologia
Osso Semilunar/transplante
Osteonecrose/cirurgia
[Mh] Termos MeSH secundário: Adulto
Artroscopia
Transplante Ósseo
Sobrevivência Celular
Feminino
Retalhos de Tecido Biológico
Seres Humanos
Articulação do Punho/cirurgia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.2106/JBJS.CC.16.00023


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[PMID]:29305868
[Au] Autor:Tominaga A; Sato M; Takahashi T; Toyoda E; Toyoda K; Suzuki T; Takahashi M; Watanabe M; Okazaki K
[Ad] Endereço:Department of Orthopaedic Surgery, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjuku-ku, Tokyo, 162-8666, Japan.
[Ti] Título:Quality assessment of cellular and tissue-based products using liquid chromatography-tandem mass spectrometry.
[So] Source:Biochem Biophys Res Commun;496(2):429-435, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We are currently conducting clinical research on cell sheets for cartilage regeneration. One issue with the future use of chondrocyte sheets as cellular and tissue-based products is quality assessment. Currently, chondrocyte sheets are evaluated using invasive methods that cannot be performed on every sheet produced. We report here on our liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique that allows the noninvasive assessment of every sheet using only 50 µl of culture medium. We found that LC-MS/MS could be used to confirm cell sheet viability through the measurement of glucose and glutamine uptake, to estimate extracellular matrix production by measuring serine consumption, to estimate cell kinetics by measuring cytidine and uracil concentrations, and to estimate melanoma inhibitory activity level by measuring pyridoxal concentration. LC-MS/MS may be useful for the noninvasive assessment of products to be used in regenerative medicine.
[Mh] Termos MeSH primário: Cartilagem/metabolismo
Condrócitos/metabolismo
Cromatografia Líquida/normas
Regeneração/fisiologia
Espectrometria de Massas em Tandem/normas
[Mh] Termos MeSH secundário: Transporte Biológico
Cartilagem/patologia
Cartilagem/cirurgia
Citidina/metabolismo
Matriz Extracelular
Glucose/metabolismo
Glutamina/metabolismo
Seres Humanos
Controle de Qualidade
Medicina Regenerativa/métodos
Serina/metabolismo
Uracila/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0RH81L854J (Glutamine); 452VLY9402 (Serine); 56HH86ZVCT (Uracil); 5CSZ8459RP (Cytidine); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE


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[PMID]:28468151
[Au] Autor:Pickrell BB; Meaike JD; Cañadas KT; Chandy BM; Buchanan EP
[Ad] Endereço:*Division of Plastic Surgery, Michael E. DeBakey Department of Surgery, Baylor College of Medicine †Department of Otolaryngology, Texas Children's Hospital, Houston, TX.
[Ti] Título:Tracheal Cartilaginous Sleeve in Syndromic Craniosynostosis: An Underrecognized Source of Significant Morbidity and Mortality.
[So] Source:J Craniofac Surg;28(3):696-699, 2017 May.
[Is] ISSN:1536-3732
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tracheal cartilaginous sleeve (TCS) is a rare and previously unrecognized source of morbidity and mortality in patients with certain craniosynostosis syndromes. There is a paucity of reporting on this airway anomaly, and the true incidence of TCS is largely unknown. The purpose of this study was to investigate the incidence of TCS among patients with syndromic craniosynostosis at our institution. Patients with syndromic craniosynostosis who underwent direct bronchoscopy and laryngoscopy were evaluated retrospectively by pediatric otolaryngologists for the presence of TCS and associated anomalies. Among patients with a diagnosis of syndromic craniosynostosis in our craniofacial database, 10 (37%) were found to have previous direct bronchoscopy and laryngoscopy reports. Of these 10 patients, 2 had Crouzon syndrome, 3 had Pfeiffer syndrome, 3 had Apert syndrome, 1 had Muenke syndrome, and 1 had Antley-Bixler syndrome. Eighty percent (8/10) of these patients were found to have some evidence of TCS. The most commonly observed associated findings included the following: tracheostomy dependency (7/10; 70%), hearing loss (6/10; 60%), obstructive sleep apnea (5/10; 50%), cervical spine anomalies (5/10; 50%), developmental delay (5/10; 50%), and enlarged cerebral ventricles (4/10; 40%). Larger multicenter studies are required to further characterize this airway anomaly and its impact on this patient population. Our results confirm the importance of thorough airway evaluation at initial presentation and the need for validated screening protocols.
[Mh] Termos MeSH primário: Anormalidades Múltiplas
Cartilagem/anormalidades
Craniossinostoses/diagnóstico
Apneia Obstrutiva do Sono/epidemiologia
Traqueia/anormalidades
Doenças da Traqueia/congênito
[Mh] Termos MeSH secundário: Cartilagem/cirurgia
Criança
Pré-Escolar
Craniossinostoses/epidemiologia
Craniossinostoses/cirurgia
Feminino
Seres Humanos
Masculino
Morbidade/tendências
Estudos Retrospectivos
Apneia Obstrutiva do Sono/etiologia
Apneia Obstrutiva do Sono/cirurgia
Taxa de Sobrevida/tendências
Traqueia/cirurgia
Doenças da Traqueia/diagnóstico
Doenças da Traqueia/epidemiologia
Traqueostomia/métodos
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1097/SCS.0000000000003489


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[PMID]:29025654
[Au] Autor:Cheng Z; Landish B; Chi Z; Nannan C; Jingyu D; Sen L; Xiangjin L
[Ad] Endereço:Department of Orthopaedic Orthopaedics, First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China; The Sport Medicine Center of the First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou
[Ti] Título:3D printing hydrogel with graphene oxide is functional in cartilage protection by influencing the signal pathway of Rank/Rankl/OPG.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:244-252, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:3Dprinting is defined as the use of printing technology to deposit living cells, and biomaterials on a given /a substrate. Graphene oxide nanoparticles (GO-np) have been used as a delivery vehicle for small molecule drugs in order to investigate the state of GO-np within 3D tissue constructs in terms of a composite 3D printing scaffold, which in turn is relevant to the protection of cartilage. We transplanted rats with hydrogel/GO-np and hydrogel, which in turn showed that hydrogel/GO-np protected the tissue of cartilage by the signal pathway of Rank/Rankl/OPG. Those findings indicated that GO-np may be potentially used to control the release of carrier materials and influence the signal pathway of Rank/Rankl/OPG.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Cartilagem/metabolismo
Grafite/química
Hidrogéis/química
Osteoprotegerina/metabolismo
Ligante RANK/metabolismo
Receptor Ativador de Fator Nuclear kappa-B/metabolismo
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/farmacologia
Proteína Morfogenética Óssea 7/química
Proteína Morfogenética Óssea 7/metabolismo
Proteína Morfogenética Óssea 7/farmacologia
Cartilagem/efeitos dos fármacos
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Condrócitos/citologia
Condrócitos/efeitos dos fármacos
Condrócitos/metabolismo
Portadores de Fármacos/química
Seres Humanos
Masculino
Nanopartículas/química
Óxidos/química
Ratos
Ratos Sprague-Dawley
Transdução de Sinais/efeitos dos fármacos
Tecidos Suporte/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Bone Morphogenetic Protein 7); 0 (Drug Carriers); 0 (Hydrogels); 0 (Osteoprotegerin); 0 (Oxides); 0 (RANK Ligand); 0 (Receptor Activator of Nuclear Factor-kappa B); 7782-42-5 (Graphite)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


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[PMID]:28468197
[Au] Autor:Yegin Y; Çelik M; Altintas A; Çolak C; Kayhan FT
[Ad] Endereço:*Department of Otorhinolaryngology, Head and Neck Surgery, Bakirköy Dr Sadi Konuk Training and Research Hospital, Istanbul †Department of Otorhinolaryngology, Head and Neck Surgery, Iskilip Atif Hoca State Hospital, Çorum ‡Department of Radiology, Bakirköy Dr Sadi Konuk Training and Research Hospital, Istanbul, Turkey.
[Ti] Título:Do the Angle and Length of the Eustachian Tube Affect the Success Rate of Pediatric Cartilage Type 1 Tympanoplasty?
[So] Source:J Craniofac Surg;28(3):e227-e231, 2017 May.
[Is] ISSN:1536-3732
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To investigate the relationships between the angle and length of the Eustachian tube (ET) (the ETa and the ETl) and the success rates of pediatric type 1 tympanoplasty. STUDY DESIGN: A retrospective clinical chart review. METHODS: In total, 51 children (31 females and 20 males; average age, 11.92 ±â€Š3.46 years; age range: 7-18 years) who underwent cartilage type 1 tympanoplasty were included. Demographics and anatomical outcomes were recorded. The patients were divided into 2 groups in terms of anatomical success. In group A, all patients had intact grafts without perforation, retraction, or lateralization, and a dry ear, at 6 months postoperatively. In group B, reperforation of the tympanic membrane was evident 6 months postoperatively. The average ETa and ETl values of each group were measured on computed tomography images using a multiplanar reconstruction technique, and compared. RESULTS: The ETa values of diseased ears of males and females were, respectively, 26.60 ±â€Š6.42° and 23.29 ±â€Š6.51°, compared to 27.25 ±â€Š5.23° and 23.32 ±â€Š4.61° for normal male and female ears, respectively. In group A, the ETa was 26.46 ±â€Š6.82° in males and 22.95 ±â€Š7.50° in females. In group B, the ETa was 26.85 ±â€Š6.12° in males and 23.90 ±â€Š4.45° in females. In group A, the mean ETl was 41.0 mm (29.6-45.3 mm) in males and 37.9 mm (32.0-44.5 mm) in females. In group B, the mean ETl was 40.5 mm (30.5-47.1 mm) in males and 38.0 mm (32.8-45.0 mm) in females. In group A, the ETa value of diseased ears did not differ between females and males, but in normal ears, the ETa was higher in males than females (P = 0.020 and P < 0.05, respectively). In group B, no difference was evident between the ETa values of normal and diseased ears (P > 0.05). No difference in the ETl values of diseased and normal ears, in either group, was apparent between females and males (both P > 0.05). CONCLUSIONS: Neither the ETa nor the ETl affected the success rate of pediatric cartilage type 1 tympanoplasty. Further studies with larger numbers of patients are needed to compare anatomical outcomes after placement of various graft types and the effects of anatomical features of the ET on the success rate of pediatric tympanoplasty.
[Mh] Termos MeSH primário: Tuba Auditiva/patologia
Perfuração da Membrana Timpânica/cirurgia
Timpanoplastia/métodos
[Mh] Termos MeSH secundário: Adolescente
Cartilagem/transplante
Criança
Feminino
Seres Humanos
Masculino
Estudos Retrospectivos
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1097/SCS.0000000000003434


  10 / 22062 MEDLINE  
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[PMID]:29197591
[Au] Autor:Mahboudi H; Kazemi B; Soleimani M; Hanaee-Ahvaz H; Ghanbarian H; Bandehpour M; Enderami SE; Kehtari M; Barati G
[Ad] Endereço:Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
[Ti] Título:Enhanced chondrogenesis of human bone marrow mesenchymal Stem Cell (BMSC) on nanofiber-based polyethersulfone (PES) scaffold.
[So] Source:Gene;643:98-106, 2018 Feb 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mesenchymal stem cells (MSC) from bone marrow hold great potential as a cell source for cartilage repair. The objective of our study was differentiation of MSC toward chondrocyte by using Nanofiber-based polyethersulfone (PES) scaffold and also enhanced chondrogenic differentiation of BMSC in vitro. MSCs were harvested from bone marrow of human and PES scaffold was fabricated via Electrospinning. The isolated cells were cultured on the PES scaffold and scaffold free method. After 21days, Real-time PCR was performed to evaluate the cartilage-specific genes in the mRNA levels. Also, in order to confirm our results, we have done immunocytochemistry and SEM imaging. Flowcytometry confirmed the nature of the isolated adherent cells. Immunocytochemistry and SEM imaging confirmed the differentiation of MSC toward chondrocyte. Also, real time PCR showed a significant increased gene expression of collagen type II and aggrecan on the PES scaffold method when compared to the mRNA levels measured in scaffold free method. Down regulation of Collagen type I was observed in PES scaffold compared to scaffold free at day 21. Also, both methods showed a similar pattern of expression of SOX9. Our results showed that PES scaffold maintains BMSC proliferation and differentiation, and can significantly enhance chondrogenic differentiation of BMSC. PES scaffold seeded BMSC showed the highest capacity for differentiation into chondrocyte-like cells.
[Mh] Termos MeSH primário: Condrogênese/fisiologia
Células Mesenquimais Estromais/metabolismo
Polímeros/metabolismo
Sulfonas/metabolismo
[Mh] Termos MeSH secundário: Células da Medula Óssea/citologia
Cartilagem/metabolismo
Diferenciação Celular/genética
Proliferação Celular
Separação Celular
Células Cultivadas
Condrócitos/metabolismo
Seres Humanos
Nanofibras
Polímeros/uso terapêutico
Sulfonas/uso terapêutico
Tecidos Suporte
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polymers); 0 (Sulfones); 25667-42-9 (polyether sulfone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171204
[St] Status:MEDLINE



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