Base de dados : MEDLINE
Pesquisa : A02.165.257 [Categoria DeCS]
Referências encontradas : 21 [refinar]
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  1 / 21 MEDLINE  
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[PMID]:27090206
[Au] Autor:Wu W; Lin J; Wang S; Li Y; Liu M; Liu G; Cai J; Chen G; Chen R
[Ad] Endereço:Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education Fujian Normal University, Fuzhou, Fujian, 350007, China.
[Ti] Título:A novel multiphoton microscopy images segmentation method based on superpixel and watershed.
[So] Source:J Biophotonics;10(4):532-541, 2017 Apr.
[Is] ISSN:1864-0648
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Multiphoton microscopy (MPM) imaging technique based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) shows fantastic performance for biological imaging. The automatic segmentation of cellular architectural properties for biomedical diagnosis based on MPM images is still a challenging issue. A novel multiphoton microscopy images segmentation method based on superpixels and watershed (MSW) is presented here to provide good segmentation results for MPM images. The proposed method uses SLIC superpixels instead of pixels to analyze MPM images for the first time. The superpixels segmentation based on a new distance metric combined with spatial, CIE Lab color space and phase congruency features, divides the images into patches which keep the details of the cell boundaries. Then the superpixels are used to reconstruct new images by defining an average value of superpixels as image pixels intensity level. Finally, the marker-controlled watershed is utilized to segment the cell boundaries from the reconstructed images. Experimental results show that cellular boundaries can be extracted from MPM images by MSW with higher accuracy and robustness.
[Mh] Termos MeSH primário: Algoritmos
Processamento de Imagem Assistida por Computador/métodos
Microscopia de Fluorescência por Excitação Multifotônica/métodos
Reconhecimento Automatizado de Padrão/métodos
[Mh] Termos MeSH secundário: Animais
Biópsia
Cartilagem da Orelha/citologia
Cartilagem Elástica/citologia
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160420
[St] Status:MEDLINE
[do] DOI:10.1002/jbio.201600007


  2 / 21 MEDLINE  
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[PMID]:27138327
[Au] Autor:Tangerino Filho EP; Fachi JL; Vasconcelos IC; Dos Santos GM; Mendonça FA; de Aro AA; Pimentel ER; Esquisatto MA
[Ad] Endereço:Programa de Pós-graduação em Ciências Biomédicas, Centro Universitário Hermínio Ometto, Av. Dr. Maximiliano Baruto, 500 Jd. Universitário, 13607-339 Araras, SP, Brazil.
[Ti] Título:Effects of microcurrent therapy on excisional elastic cartilage defects in young rats.
[So] Source:Tissue Cell;48(3):224-34, 2016 Jun.
[Is] ISSN:1532-3072
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:The effects of microcurrent application on the elastic cartilage defects in the outer ear of young animals were analyzed. Sixty male Wistar rats were divided into a control (CG) and a treated group (TG). An excisional lesion was created in the right outer ear of each animal. Daily treatment was started after 24h and consisted of the application of a low-intensity (20µA) continuous electrical current to the site of injury for 5min. The animals were euthanized after 7, 14 and 28 days of injury and the samples were submitted to analyses. In CG, areas of newly formed cartilage and intense basophilia were seen at 28 days, while in TG the same observations were made already at 14 days. The percentage of birefringent collagen fibers was higher in CG at 28 days. The number of connective tissue cells and granulocytes was significantly higher in TG. Ultrastructural analysis revealed the presence of chondrocytes in TG at 14 days, while these cells were observed in CG only at 28 days. Cuprolinic blue staining and the amount of glycosaminoglycans were significantly higher in TG at 14 days and 28 days. The amount of hydroxyproline was significantly higher in TG at all time points studied. The active isoform of MMP-2 was higher activity in TG at 14 days. Immunoblotting for type II collagen and decorin was positive in both groups and at all time points. The treatment stimulated the proliferation and differentiation of connective tissue cells, the deposition of glycosaminoglycans and collagen, and the structural reorganization of these elements during elastic cartilage repair.
[Mh] Termos MeSH primário: Diferenciação Celular/efeitos da radiação
Proliferação Celular/efeitos da radiação
Orelha Externa/efeitos da radiação
Cartilagem Elástica/efeitos da radiação
[Mh] Termos MeSH secundário: Animais
Cartilagem Articular/crescimento & desenvolvimento
Cartilagem Articular/efeitos da radiação
Condrócitos/efeitos da radiação
Colágeno/metabolismo
Orelha Externa/crescimento & desenvolvimento
Orelha Externa/lesões
Cartilagem Elástica/crescimento & desenvolvimento
Radiação Eletromagnética
Masculino
Ratos
Cicatrização/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-34-5 (Collagen)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170324
[Lr] Data última revisão:
170324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160504
[St] Status:MEDLINE


  3 / 21 MEDLINE  
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[PMID]:26903410
[Au] Autor:Ling Y; Li C; Feng K; Duncan R; Eisma R; Huang Z; Nabi G
[Ad] Endereço:Academic Section of Urology, Division of Imaging Technology, School of Medicine, University of Dundee, Dundee DD1 9SY, Scotland, UK; School of Engineering, Physics and Mathematics, University of Dundee, Dundee DD1 4HN, Scotland, UK.
[Ti] Título:Effects of fixation and preservation on tissue elastic properties measured by quantitative optical coherence elastography (OCE).
[So] Source:J Biomech;49(7):1009-1015, 2016 05 03.
[Is] ISSN:1873-2380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fixed and preserved tissues have been massively used in the development of biomedical equipment and instrumentation. Not only the tissue morphology, but also its mechanical properties need to be considered in the fixation and preservation procedures since mechanical properties have significant influence on the design and performance of such instruments. Understanding the effects of storage and preservation conditions on the mechanical properties of soft tissue has both clinical and experimental significance. To this end, we aimed to study the effects of tissue preservation (by 10% formalin and Thiel fluids) on the elastic properties of five different kinds of fresh tissues from pig and chicken; specifically fat, liver, muscle, tendon and cartilage. The tissue elasticity was measured intensively and strictly within a controlled timeline of 6 months by quantitative optical coherence elastography (OCE) system. Our findings suggest that the elasticity change of tissues in the formalin solution has an ascending trend, but that of Thiel remains almost constant, providing a more real texture and properties.
[Mh] Termos MeSH primário: Tecido Elástico
Técnicas de Imagem por Elasticidade/métodos
Preservação de Tecido
[Mh] Termos MeSH secundário: Tecido Adiposo/fisiologia
Animais
Cartilagem/fisiologia
Galinhas
Cartilagem Elástica
Elasticidade
Fígado/fisiologia
Músculos/fisiologia
Suínos
Tendões/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170805
[Lr] Data última revisão:
170805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160224
[St] Status:MEDLINE


  4 / 21 MEDLINE  
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[PMID]:26375221
[Au] Autor:Karabulut YY; Senel E; Karabulut HH; Dölek Y
[Ad] Endereço:Mersin University, Mersin, TR.
[Ti] Título:Three different clinical faces of the same histopathological entity: hair follicle nevus, trichofolliculoma and accessory tragus.
[So] Source:An Bras Dermatol;90(4):519-22, 2015 Jul-Aug.
[Is] ISSN:1806-4841
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hair follicle nevus is a rare, congenital hamartoma with follicular differentiation characterized histologically by numerous, tiny, mature hair follicles. Trichofolliculoma, the histopathological features of which are quite similar to those of hair follicle nevus, is also a hamartoma that differs from hair follicle. Accessory tragus is a relatively common, benign congenital abnormality of the external ear with an incidence rate of 1 to 10 per 1,000 live births. OBJECTIVE: This study seeks to assess the discriminatory value of currently available, histological criteria in the differential diagnosis of hair follicle nevus, accessory tragi and trichofolliculoma. METHODS: Twenty-one patients comprising 9 cases of hair follicle nevus, 8 accessory tragi patients and 4 trichofolliculoma cases, were recruited to perform the study. RESULTS: There were 10 males and 11 females in the study group. No significant difference was observed between the three study groups in terms of age, gender or histopathological parameters such as density of hair follicles, subcutaneous fat score and presence of connective tissue framework. Cartilaginous component was seen in 8 cases that were diagnosed as accessory tragi, while central cyst and radiating hair follicles were seen in 4 cases which were diagnosed as trichofolliculoma. CONCLUSION: The results of our study showed that diagnostic discrimination of these diseases could be made only with the clinicopathologic correlation because of their clinical and histopathological similarities.
[Mh] Termos MeSH primário: Orelha Externa/anormalidades
Orelha Externa/patologia
Cisto Folicular/patologia
Doenças do Cabelo/patologia
Folículo Piloso/patologia
Hamartoma/patologia
Neoplasia de Células Basais/patologia
Neoplasias Cutâneas/patologia
[Mh] Termos MeSH secundário: Tecido Adiposo/patologia
Adolescente
Adulto
Idoso
Criança
Pré-Escolar
Cartilagem Elástica/patologia
Feminino
Seres Humanos
Lactente
Masculino
Meia-Idade
Nevo/patologia
Estudos Retrospectivos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150918
[Lr] Data última revisão:
150918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150917
[St] Status:MEDLINE


  5 / 21 MEDLINE  
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[PMID]:26238630
[Au] Autor:Zhang X; Xue K; Zhou J; Xu P; Huang H; Liu K
[Ad] Endereço:Shanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
[Ti] Título:Chondrogenic differentiation of bone marrow­derived stem cells cultured in the supernatant of elastic cartilage cells.
[So] Source:Mol Med Rep;12(4):5355-60, 2015 Oct.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Repair of cartilage defects remains a challenge for surgeons, owing to its poor self­repairing capacity. Cartilage tissue engineering, particularly marrow stem cell­based cartilage regeneration, provides a promising option for the regeneration of damaged cartilage. Although producing tissue­engineered cartilage from marrow stem cells appeared to be a feasible method, constructing certain sub­types of cartilage, including elastic cartilage, remains difficult. Therefore, the present study explored the feasibility of constructing elastic cartilage by culturing bone marrow­derived stem cells (BMSCs) in the supernatant of elastic cartilage cells to generate elastic cartilage. The elastic cartilage cells were obtained from the auricle cartilage of a newborn pig, and BMSCs were isolated from pig bone marrow aspirate. The supernatant of the chondrocytes was collected and then used to the culture BMSCs. At various time­points, the differentiation of BMSCs was evaluated by gross view, histological examination and quantitative polymerase chain reaction. BMSCs changed from spindle­shaped cells into polygonal cells with increasing culture time. The expression of collagen II and elastin was observed in the cells cultured in the supernatant of elastic chondrocytes, while no expression was observed in the control cells. Furthermore, the expression of collagen I and collagen X was downregulated in the cells cultured in the supernatant of elastic cartilage cells. The supernatant of elastic cartilage cells promoted the differentiation of BMSCs into elastic cartilage cells, which may be a promising method for constructing certain sub­types of tissue­engineered cartilage.
[Mh] Termos MeSH primário: Diferenciação Celular/efeitos dos fármacos
Condrócitos/citologia
Condrócitos/efeitos dos fármacos
Condrogênese/efeitos dos fármacos
Meios de Cultivo Condicionados/farmacologia
Cartilagem Elástica/citologia
Cartilagem Elástica/metabolismo
Células Mesenquimais Estromais/citologia
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Biomarcadores
Diferenciação Celular/genética
Condrogênese/genética
Expressão Gênica
Imunofenotipagem
Proteínas de Membrana
Células Mesenquimais Estromais/metabolismo
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Culture Media, Conditioned); 0 (Membrane Proteins)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150924
[Lr] Data última revisão:
150924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150805
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2015.4113


  6 / 21 MEDLINE  
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[PMID]:25626816
[Au] Autor:Liao HT; Zheng R; Liu W; Zhang WJ; Cao Y; Zhou G
[Ad] Endereço:Shanghai, People's Republic of China; and Taoyuan, Taiwan From the Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China; and the Department of Plastic and Reconstructive Surgery, Craniofacial Research Center, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University.
[Ti] Título:Prefabricated, ear-shaped cartilage tissue engineering by scaffold-free porcine chondrocyte membrane.
[So] Source:Plast Reconstr Surg;135(2):313e-21e, 2015 Feb.
[Is] ISSN:1529-4242
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Ear defects caused by traumatic injury, tumor ablation, and congenital deficiency are still challenging problems for the plastic and reconstructive surgeon. The authors developed a scaffold-free, ear-shaped cartilage by tailoring a multilayered chondrocyte membrane on an ear-shaped titanium alloy model and investigated the possibility of long-term ear-shaped maintenance in nude mice. METHODS: High-density chondrocytes (approximately 30 × 10 cells) were seeded to produce chondrocyte membranes after cultivation under chondrogenic medium for 2 weeks. Then, three-layer chondrocyte membranes were tailored on the ear-shaped titanium mold and fixed by 6-0 nylon. The constructs were implanted onto the dorsal pockets of nude mice for 8 and 24 weeks. The chondrocyte membrane, 8- and 24-week implants were analyzed by safranin O, toluidine blue, elastica van Gieson, and collagen type II immunohistochemistry stains and quantitative measurement of glycosaminoglycan and total collagen compared with native cartilage. Mechanical strength was compared by compressive Young's modulus. RESULTS: Results showed that the chondrocyte membrane was durable and nonfragile and easily manipulated by forceps. The composite of chondrocyte membrane and titanium alloy maintained the stable ear-like shape after 8 and 24 weeks of subcutaneous implantation. Histologic examination verified that the newly formed tissue at the implant construct was elastic cartilage at both 8 and 24 weeks by safranin O, toluidine blue, elastica van Gieson, and collagen type II immunohistochemistry stains. The Young's modulus was only half of and similar to normal cartilage in 8- and 24-week implants, respectively. CONCLUSION: This study demonstrated that an ear-shaped elastic cartilage could be regenerated by a scaffold-free chondrocyte membrane shaped by a prefabricated, three-dimensional, ear-shaped titanium mold.
[Mh] Termos MeSH primário: Membrana Celular/ultraestrutura
Condrócitos/ultraestrutura
Orelha Externa
Cartilagem Elástica/citologia
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Ligas
Animais
Animais Recém-Nascidos
Células Cultivadas
Colágeno/biossíntese
Meios de Cultura Livres de Soro
Módulo de Elasticidade
Glicosaminoglicanos/biossíntese
Seres Humanos
Teste de Materiais
Camundongos
Camundongos Nus
Próteses e Implantes
Stents
Sus scrofa
Suínos
Titânio
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VIDEO-AUDIO MEDIA
[Nm] Nome de substância:
0 (Alloys); 0 (Culture Media, Serum-Free); 0 (Glycosaminoglycans); 9007-34-5 (Collagen); D1JT611TNE (Titanium)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:150624
[Lr] Data última revisão:
150624
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:150129
[St] Status:MEDLINE
[do] DOI:10.1097/PRS.0000000000001105


  7 / 21 MEDLINE  
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[PMID]:24815164
[Au] Autor:Mizuno M; Takebe T; Kobayashi S; Kimura S; Masutani M; Lee S; Jo YH; Lee JI; Taniguchi H
[Ad] Endereço:Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
[Ti] Título:Elastic cartilage reconstruction by transplantation of cultured hyaline cartilage-derived chondrocytes.
[So] Source:Transplant Proc;46(4):1217-21, 2014 May.
[Is] ISSN:1873-2623
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Current surgical intervention of craniofacial defects caused by injuries or abnormalities uses reconstructive materials, such as autologous cartilage grafts. Transplantation of autologous tissues, however, places a significant invasiveness on patients, and many efforts have been made for establishing an alternative graft. Recently, we and others have shown the potential use of reconstructed elastic cartilage from ear-derived chondrocytes or progenitors with the unique elastic properties. Here, we examined the differentiation potential of canine joint cartilage-derived chondrocytes into elastic cartilage for expanding the cell sources, such as hyaline cartilage. Articular chondrocytes are isolated from canine joint, cultivated, and compared regarding characteristic differences with auricular chondrocytes, including proliferation rates, gene expression, extracellular matrix production, and cartilage reconstruction capability after transplantation. Canine articular chondrocytes proliferated less robustly than auricular chondrocytes, but there was no significant difference in the amount of sulfated glycosaminoglycan produced from redifferentiated chondrocytes. Furthermore, in vitro expanded and redifferentiated articular chondrocytes have been shown to reconstruct elastic cartilage on transplantation that has histologic characteristics distinct from hyaline cartilage. Taken together, cultured hyaline cartilage-derived chondrocytes are a possible cell source for elastic cartilage reconstruction.
[Mh] Termos MeSH primário: Condrócitos/transplante
Condrogênese
Cartilagem Elástica/metabolismo
Cartilagem Hialina/transplante
Regeneração
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Animais
Autoenxertos
Diferenciação Celular
Proliferação Celular
Células Cultivadas
Condrócitos/metabolismo
Condrogênese/genética
Cães
Regulação da Expressão Gênica
Glicosaminoglicanos/metabolismo
Cartilagem Hialina/citologia
Cartilagem Hialina/metabolismo
Masculino
Regeneração/genética
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glycosaminoglycans)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:140512
[Lr] Data última revisão:
140512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140513
[St] Status:MEDLINE


  8 / 21 MEDLINE  
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[PMID]:24124666
[Au] Autor:Giardini-Rosa R; Joazeiro PP; Thomas K; Collavino K; Weber J; Waldman SD
[Ad] Endereço:1 Human Mobility Research Centre, Kingston General Hospital and Queen's University , Kingston, Canada .
[Ti] Título:Development of scaffold-free elastic cartilaginous constructs with structural similarities to auricular cartilage.
[So] Source:Tissue Eng Part A;20(5-6):1012-26, 2014 Mar.
[Is] ISSN:1937-335X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:External ear reconstruction with autologous cartilage still remains one of the most difficult problems in the fields of plastic and reconstructive surgery. As the absence of tissue vascularization limits the ability to stimulate new tissue growth, relatively few surgical approaches are currently available (alloplastic implants or sculpted autologous cartilage grafts) to repair or reconstruct the auricle (or pinna) as a result of traumatic loss or congenital absence (e.g., microtia). Alternatively, tissue engineering can offer the potential to grow autogenous cartilage suitable for implantation. While tissue-engineered auricle cartilage constructs can be created, a substantial number of cells are required to generate sufficient quantities of tissue for reconstruction. Similarly, as routine cell expansion can elicit negative effects on chondrocyte function, we have developed an approach to generate large-sized engineered auricle constructs (≥3 cm(2)) directly from a small population of donor cells (20,000-40,000 cells/construct). Using rabbit donor cells, the developed bioreactor-cultivated constructs adopted structural-like characteristics similar to native auricular cartilage, including the development of distinct cartilaginous and perichondrium-like regions. Both alterations in media composition and seeding density had profound effects on the formation of engineered elastic tissue constructs in terms of cellularity, extracellular matrix accumulation, and tissue structure. Higher seeding densities and media containing sodium bicarbonate produced tissue constructs that were closer to the native tissue in terms of structure and composition. Future studies will be aimed at improving the accumulation of specific tissue constituents and determining the clinical effectiveness of this approach using a reconstructive animal model.
[Mh] Termos MeSH primário: Cartilagem da Orelha/anatomia & histologia
Cartilagem da Orelha/fisiologia
Cartilagem Elástica/anatomia & histologia
Cartilagem Elástica/fisiologia
Engenharia Tecidual/métodos
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Animais
Reatores Biológicos
Colágeno/metabolismo
Cartilagem da Orelha/ultraestrutura
Cartilagem Elástica/ultraestrutura
Elastina/metabolismo
Matriz Extracelular/metabolismo
Feminino
Imuno-Histoquímica
Proteoglicanas/metabolismo
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Proteoglycans); 9007-34-5 (Collagen); 9007-58-3 (Elastin)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131016
[St] Status:MEDLINE
[do] DOI:10.1089/ten.TEA.2013.0159


  9 / 21 MEDLINE  
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[PMID]:24038678
[Au] Autor:Mizuno M; Kobayashi S; Takebe T; Kan H; Yabuki Y; Matsuzaki T; Yoshikawa HY; Nakabayashi S; Ik LJ; Maegawa J; Taniguchi H
[Ad] Endereço:Department of Regenerative Medicine, Kanagawa Children's Medical Center, Yokohama, Kanagawa, Japan.
[Ti] Título:Brief report: reconstruction of joint hyaline cartilage by autologous progenitor cells derived from ear elastic cartilage.
[So] Source:Stem Cells;32(3):816-21, 2014 Mar.
[Is] ISSN:1549-4918
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In healthy joints, hyaline cartilage covering the joint surfaces of bones provides cushioning due to its unique mechanical properties. However, because of its limited regenerative capacity, age- and sports-related injuries to this tissue may lead to degenerative arthropathies, prompting researchers to investigate a variety of cell sources. We recently succeeded in isolating human cartilage progenitor cells from ear elastic cartilage. Human cartilage progenitor cells have high chondrogenic and proliferative potential to form elastic cartilage with long-term tissue maintenance. However, it is unknown whether ear-derived cartilage progenitor cells can be used to reconstruct hyaline cartilage, which has different mechanical and histological properties from elastic cartilage. In our efforts to develop foundational technologies for joint hyaline cartilage repair and reconstruction, we conducted this study to obtain an answer to this question. We created an experimental canine model of knee joint cartilage damage, transplanted ear-derived autologous cartilage progenitor cells. The reconstructed cartilage was rich in proteoglycans and showed unique histological characteristics similar to joint hyaline cartilage. In addition, mechanical properties of the reconstructed tissues were higher than those of ear cartilage and equal to those of joint hyaline cartilage. This study suggested that joint hyaline cartilage was reconstructed from ear-derived cartilage progenitor cells. It also demonstrated that ear-derived cartilage progenitor cells, which can be harvested by a minimally invasive method, would be useful for reconstructing joint hyaline cartilage in patients with degenerative arthropathies.
[Mh] Termos MeSH primário: Orelha/anatomia & histologia
Cartilagem Elástica/citologia
Cartilagem Hialina/patologia
Articulações/patologia
Medicina Regenerativa
Transplante de Células-Tronco
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Animais
Separação Celular
Células Clonais
Cães
Seres Humanos
Transplante Autólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1410
[Cu] Atualização por classe:140219
[Lr] Data última revisão:
140219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130917
[St] Status:MEDLINE
[do] DOI:10.1002/stem.1529


  10 / 21 MEDLINE  
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[PMID]:24322024
[Au] Autor:Muhlfay G; Cotoi OS; Marginean C; Lostun G; Mózes H; Horváth KU
[Ad] Endereço:Department of Cell and Molecular Biology, University of Medicine and Pharmacy of Tirgu Mures, Romania; ovidiu.cotoi@umftgm.ro.
[Ti] Título:Pilot preliminary study on the morpho-functional integration level of the auricle elastic cartilage transplanted in different tissue structures.
[So] Source:Rom J Morphol Embryol;54(3 Suppl):763-7, 2013.
[Is] ISSN:1220-0522
[Cp] País de publicação:Romania
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The nose is a complex and defining organ not only for its respiratory, olfactory and phonatory function but also for facial esthetics. It is plastic and reconstructive techniques are at the same time an otorhinolaryngological issue and an interdisciplinary one. MATERIALS AND METHODS: Among the materials used for reconstructive-reparatory surgery of the nose, we can distinguish the elastic ear cartilage transplant from patients own auricle. By analogy, we used in our experiment the rabbit ear as donor site and three radically different types of tissue as integrating structures. The modifications of the cartilages transplanted into abdominal subcutaneous tissue, muscles of the hip and under the skull periosteum were monitored through monthly sacrificing of the experimental animals. RESULTS: No matter of the presence or lack of own perichondrium coverage the cartilage pieces showed radical transformation to total changes in structure from perichondral fibrosis to endochondral ossification and even complete resorption. CONCLUSIONS: We consider that at least a part of the reconstructive and esthetic plastic surgery failure comes from not knowing these significant changes that take place on the insertion site of own transplanted cartilage. The future surgical guidelines should remember the phenomena described for the first time in our research.
[Mh] Termos MeSH primário: Pavilhão Auricular/anatomia & histologia
Pavilhão Auricular/transplante
Cartilagem da Orelha/anatomia & histologia
Cartilagem da Orelha/transplante
Cartilagem Elástica/anatomia & histologia
Cartilagem Elástica/transplante
[Mh] Termos MeSH secundário: Animais
Especificidade de Órgãos
Projetos Piloto
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1407
[Cu] Atualização por classe:131210
[Lr] Data última revisão:
131210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131211
[St] Status:MEDLINE



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