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Pesquisa : A02.165.407 [Categoria DeCS]
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[PMID]:29443763
[Au] Autor:Zhang Z; Yang Z; Pan Q; Qin X; Deng Y; Cao Y
[Ad] Endereço:Jinhua Eye Hospital, Jinhua, ZheJiang.
[Ti] Título:Epibulbar complex cartilaginous choristoma: A distinctive clinicopathological case series and literature review.
[So] Source:Medicine (Baltimore);97(7):e9902, 2018 Feb.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To newly describe the clinical and histopathological characteristics of epibulbar complex cartilaginous choristoma incidentally observed in a series of pterygium excision patients.Noncomparative case series with chart review of 8 patients.During a 4-year period, we identified 8 cases of conventional unilateral nasal subpterygial cartilaginous choristoma in 1799 pterygium patients and analyzed their clinicopathological features. The incidence rate of this entity is 0.44% in pterygium patients. All of the cartilaginous choristomas were buried deep in the caruncle, covered by the pterygium, and embedded in tenon facia tissue. Its clinicopathological characteristics include hyaline cartilaginous tissue that is surrounded by fibrous connective tissue and smooth muscle bundles. S-100 protein-staining specifically revealed chondrocytes embedded within chondroid matrix.Epibulbar complex cartilaginous choristoma covered by pterygia and predominantly observed in the older population is rare. The lesions were buried deep in the caruncle, covered by the pterygium and embedded in tenon fascia tissue. These findings are inconsistent with those in previous reports.
[Mh] Termos MeSH primário: Coristoma
Túnica Conjuntiva
Procedimentos Cirúrgicos Oftalmológicos
Pterígio
[Mh] Termos MeSH secundário: Fatores Etários
China/epidemiologia
Coristoma/epidemiologia
Coristoma/patologia
Coristoma/cirurgia
Feminino
Seres Humanos
Cartilagem Hialina/patologia
Achados Incidentais
Masculino
Meia-Idade
Procedimentos Cirúrgicos Oftalmológicos/métodos
Procedimentos Cirúrgicos Oftalmológicos/estatística & dados numéricos
Pterígio/epidemiologia
Pterígio/patologia
Pterígio/cirurgia
Fatores de Risco
Proteínas S100/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (S100 Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009902


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[PMID]:28797093
[Au] Autor:Touraine S; Bouhadoun H; Engelke K; Laredo JD; Chappard C
[Ad] Endereço:B2OA, UMR CNRS 7052, University Paris Diderot, Paris, France.
[Ti] Título:Influence of meniscus on cartilage and subchondral bone features of knees from older individuals: A cadaver study.
[So] Source:PLoS One;12(8):e0181956, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Cartilage and subchondral bone form a functional unit. Here, we aimed to examine the effect of meniscus coverage on the characteristics of this unit in knees of older individuals. METHODS: We assessed the hyaline cartilage, subchondral cortical plate (SCP), and subchondral trabecular bone in areas covered or uncovered by the meniscus from normal cadaver knees (without degeneration). Bone cores harvested from the medial tibial plateau at locations uncovered (central), partially covered (posterior), and completely covered (peripheral) by the meniscus were imaged by micro-CT. The following were measured on images: cartilage volume (Cart.Vol, mm3) and thickness (Cart.Th, mm); SCP thickness (SCP.Th, µm) and porosity (SCP.Por, %); bone volume to total volume fraction (BV/TV, %); trabecular thickness (Tb.Th, µm), spacing (Tb.Sp, µm), and number (Tb.N, 1/mm); structure model index (SMI); trabecular pattern factor (Tb.Pf); and degree of anisotropy (DA). RESULTS: Among the 28 specimens studied (18 females) from individuals with mean age 82.8±10.2 years, cartilage and SCP were thicker at the central site uncovered by the meniscus than the posterior and peripheral sites, and Cart.Vol was greater. SCP.Por was highest in posterior samples. In the upper 1-5 mm of subchondral bone, central samples were characterized by higher values for BV/TV, Tb.N, Tb.Th, and connectivity (Tb.Pf), a more plate-like trabecular structure and lower anisotropy than with other samples. Deeper down, at 6-10 mm, the differences were slightly higher for Tb.Th centrally, DA peripherally and SMI posteriorly. CONCLUSIONS: The coverage or not by meniscus in the knee of older individuals is significantly associated with Cart.Th, SCP.Th, SCP.Por and trabecular microarchitectural parameters in the most superficial 5 mm and to a lesser extent the deepest area of subchondral trabecular bone. These results suggest an effect of differences in local loading conditions. In subchondral bone uncovered by the meniscus, the trabecular architecture resembles that of highly loaded areas.
[Mh] Termos MeSH primário: Cartilagem Articular/diagnóstico por imagem
Cartilagem Hialina/diagnóstico por imagem
Articulação do Joelho/diagnóstico por imagem
Menisco/diagnóstico por imagem
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Imagem Tridimensional
Masculino
Osteoartrite do Joelho/diagnóstico por imagem
Suporte de Carga
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181956


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[PMID]:28692374
[Au] Autor:Mirza YH; Oussedik S
[Ad] Endereço:Trauma Fellow, Department of Trauma and Orthopaedics, Royal Gwent Hospital, Newport, NP20 2UB.
[Ti] Título:Is there a role for stem cells in treating articular injury?
[So] Source:Br J Hosp Med (Lond);78(7):372-377, 2017 Jul 02.
[Is] ISSN:1750-8460
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Articular cartilage is a specialized tissue with a high prevalence of injuries. The complex architecture of articular cartilage means that injuries are difficult to treat. The sequelae of such injuries include post-traumatic osteoarthritis. Current treatments include microfracture, microdrilling, osteochondral transplantation and matrix autologous chondral implantation. However, current surgical therapies have a number of disadvantages. Mesenchymal stem cells have been suggested as a potential alternative therapy, with a theoretical ability to regenerate articular cartilage. Research, although positive, is mainly limited to case series, in which the follow up is short to medium term. Stem cells may hold the answer to the age-old problem of articular cartilage injury but more robust evidence is required.
[Mh] Termos MeSH primário: Cartilagem Articular/lesões
Condrócitos/transplante
Lesões do Quadril/terapia
Cartilagem Hialina/lesões
Traumatismos do Joelho/terapia
Transplante de Células-Tronco Mesenquimais
[Mh] Termos MeSH secundário: Tecido Adiposo/citologia
Artroscopia
Células da Medula Óssea
Transplante Ósseo
Fibrocartilagem
Seres Humanos
Cartilagem Hialina/transplante
Procedimentos Ortopédicos
Engenharia Tecidual
Transplante Autólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.12968/hmed.2017.78.7.372


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[PMID]:28666028
[Au] Autor:Zhang X; Wu S; Naccarato T; Prakash-Damani M; Chou Y; Chu CQ; Zhu Y
[Ad] Endereço:Division of Arthritis and Rheumatic Disease, Oregon Health & Science University, Portland, OR, United States of America.
[Ti] Título:Regeneration of hyaline-like cartilage in situ with SOX9 stimulation of bone marrow-derived mesenchymal stem cells.
[So] Source:PLoS One;12(6):e0180138, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microfracture, a common procedure for treatment of cartilage injury, induces fibrocartilage repair by recruiting bone marrow derived mesenchymal stem cells (MSC) to the site of cartilage injury. However, fibrocartilage is inferior biomechanically to hyaline cartilage. SRY-type high-mobility group box-9 (SOX9) is a master regulator of chondrogenesis by promoting proliferation and differentiation of MSC into chondrocytes. In this study we aimed to test the therapeutic potential of cell penetrating recombinant SOX9 protein in regeneration of hyaline cartilage in situ at the site of cartilage injury. We generated a recombinant SOX9 protein which was fused with super positively charged green fluorescence protein (GFP) (scSOX9) to facilitate cell penetration. scSOX9 was able to induce chondrogenesis of bone marrow derived MSC in vitro. In a rabbit cartilage injury model, scSOX9 in combination with microfracture significantly improved quality of repaired cartilage as shown by macroscopic appearance. Histological analysis revealed that the reparative tissue induced by microfracture with scSOX9 had features of hyaline cartilage; and collagen type II to type I ratio was similar to that in normal cartilage. This short term in vivo study demonstrated that when administered at the site of microfracture, scSOX9 was able to induce reparative tissue with features of hyaline cartilage.
[Mh] Termos MeSH primário: Células da Medula Óssea/efeitos dos fármacos
Cartilagem Hialina/efeitos dos fármacos
Células Mesenquimais Estromais/efeitos dos fármacos
Regeneração
Fatores de Transcrição SOX9/farmacologia
[Mh] Termos MeSH secundário: Animais
Células da Medula Óssea/citologia
Linhagem Celular
Proliferação Celular/efeitos dos fármacos
Seres Humanos
Cartilagem Hialina/fisiologia
Células Mesenquimais Estromais/citologia
Coelhos
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (SOX9 Transcription Factor); 0 (SOX9 protein, human)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180138


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[PMID]:28407736
[Au] Autor:Guo J; Gao JZ; Guo LJ; Yin ZX; He EX
[Ad] Endereço:Spine Surgery, The First Affiliated Hospital of Guangzhou Medical University, 151 Yanjiang West Road, Guangzhou, 510000, China.
[Ti] Título:Large enchondroma of the thoracic spine: a rare case report and review of the literature.
[So] Source:BMC Musculoskelet Disord;18(1):155, 2017 Apr 13.
[Is] ISSN:1471-2474
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Enchondroma, a subtype of chondroma, originates from the medullary cavity of the bone and produces an expansile growth pattern. Enchondroma located in the spine is rare and a few cases of large thoracic enchondroma have been reported. The authors document a rare case of large enchondroma in the thoracic spine of a 49-year-old woman, and discuss its clinical, radiological and histopathological characteristics. CASE PRESENTATION: The patient presented with rapidly progressive and severe pain on her upper back. Magnetic resonance imaging revealed an expansile lesion at the posterior elements of T3 that was hypointense on T1-weighted images and mixed iso- to hyperintense on T2-weighted images. Administration of gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA) resulted in heterogeneous enhancement. During surgery, a large tumor of 4.2cm × 4.7cm × 2.1cm was resected along with the lamina and spinous process. Histological examination revealed that the tumor consisted of mature hyaline cartilage with typical chondrocytes, indicating that it was an enchondroma. CONCLUSIONS: Despite its benign-growing nature, enchondroma should be examined closely for signs of enchondromatosis and enchondrosarcoma. Complete surgical resection is the treatment of choice for immediate relief of symptoms and avoidance of recurrence.
[Mh] Termos MeSH primário: Dor nas Costas/cirurgia
Condroma/diagnóstico
Recidiva Local de Neoplasia/prevenção & controle
Neoplasias da Coluna Vertebral/diagnóstico
Vértebras Torácicas/patologia
[Mh] Termos MeSH secundário: Dor nas Costas/etiologia
Condroma/complicações
Condroma/patologia
Condroma/cirurgia
Meios de Contraste
Feminino
Gadolínio DTPA/administração & dosagem
Seres Humanos
Cartilagem Hialina/patologia
Imagem por Ressonância Magnética
Meia-Idade
Procedimentos Ortopédicos
Osteólise/diagnóstico por imagem
Osteólise/etiologia
Radiografia Torácica
Neoplasias da Coluna Vertebral/complicações
Neoplasias da Coluna Vertebral/patologia
Neoplasias da Coluna Vertebral/cirurgia
Vértebras Torácicas/diagnóstico por imagem
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Contrast Media); K2I13DR72L (Gadolinium DTPA)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1186/s12891-017-1519-z


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[PMID]:27842586
[Au] Autor:Hubert J; Hawellek T; Hischke S; Bertrand J; Krause M; Püschel K; Rüther W; Niemeier A
[Ad] Endereço:Department of Orthopaedics, University Medical Center Hamburg-Eppendorf, Martinistraße 52, Hamburg, 20246, Germany. j.hubert@uke.de.
[Ti] Título:Hyaline cartilage calcification of the first metatarsophalangeal joint is associated with osteoarthritis but independent of age and BMI.
[So] Source:BMC Musculoskelet Disord;17(1):474, 2016 Nov 15.
[Is] ISSN:1471-2474
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hyaline cartilage calcification (CC) is associated with osteoarthritis (OA) in hip and knee joints. The first metatarsophalangeal joint (1 MTPJ) is frequently affected by OA, but it is unclear if CC occurs in the 1 MTPJ. The aim of the present study was to analyze the prevalence of CC of the 1 MTPJ in the general population by high-resolution digital contact radiography (DCR) and to determine its association with histological OA severity, age and body mass index (BMI). METHODS: 168 metatarsal heads of 84 donors (n = 47 male, n = 37 female; mean age 62.73 years, SD ±18.8, range 20-93) were analyzed by DCR for the presence of CC. Histological OA grade (hOA) by OARSI was analyzed in the central load-bearing zone of the first metatarsal head (1 MH). Structural equation modeling (SEM) was performed to analyze the interrelationship between CC, hOA, age and BMI. RESULTS: The prevalence of CC of 1 MH was 48.8 % (41/84) (95 %-CI [37.7 %, 60.0 %]), independent of the affected side (p = 0.42), gender (p = 0.41) and BMI (p = 0.51). The mean amount of CC of one MH correlated significantly with that of the contralateral side (r = 0.4, 95 %-CI [0.26, 0.52], p < 0.001). The mean amount of CC (in % of total cartilage area) of the MH correlated significantly with the severity of hOA (r = 0.51, 95 %-CI [0.32, 0.65], p < 0.001). SEM revealed significant associations between CC and hOA (r = 0.74, p < 0.001) and between hOA and age (ß = 0.62, p = 0.001), but not between CC and age (p = 0.15). There was no significant influence of BMI on either CC (p = 0.37) or hOA (p = 0.16). CONCLUSION: The observation that CC of the 1 MH is significantly associated with the severity of OA but independent of age and BMI, suggests an intimate relationship between CC and the pathogenesis of OA, the exact nature of which will have to be explored by future studies.
[Mh] Termos MeSH primário: Calcinose/etiologia
Cartilagem Hialina/patologia
Articulação Metatarsofalângica/patologia
Articulação Metatarsofalângica/fisiologia
Osteoartrite/complicações
[Mh] Termos MeSH secundário: Adulto
Fatores Etários
Idoso
Idoso de 80 Anos ou mais
Índice de Massa Corporal
Calcinose/epidemiologia
Estudos Transversais
Feminino
Seres Humanos
Masculino
Meia-Idade
Osteoartrite/patologia
Prevalência
Radiografia/métodos
Índice de Gravidade de Doença
Suporte de Carga
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161116
[St] Status:MEDLINE


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[PMID]:27833974
[Au] Autor:Radu TG; Ciurea ME; Mogoanta SS; Busuioc CJ; Grosu F; Tenovici M; Petrescu IO; Vladu IM
[Ad] Endereço:Department of Surgery, Faculty of Dentistry, University of Medicine and Pharmacy of Craiova, Romania; ssmogo@yahoo.com.
[Ti] Título:Papillary thyroid cancer stroma - histological and immunohistochemical study.
[So] Source:Rom J Morphol Embryol;57(2 Suppl):801-809, 2016.
[Is] ISSN:1220-0522
[Cp] País de publicação:Romania
[La] Idioma:eng
[Ab] Resumo:Thyroid cancer is the most frequent endocrine neoplasia. The incidence of the disease has been increasing in the past few decades in many regions, especially where the population was subject to some forms of accidental exposure. Among all the histopathological forms, papillary thyroid cancer (PTC) is the most common histological subtype of malignant thyroid tumor, representing about 80-90% of all malignant thyroid tumors. Although it is generally accepted that tumor stroma plays an essential role in the development and metastasis of tumor cells, histopathological studies focused on tumor cells characteristics. In this study, we evaluated the characteristics of tumor stroma histopathologically and immunohistochemically examining a total of 18 cases of papillary thyroid carcinomas, of which 18 cases were classic papillary carcinomas, 11 cases papillary carcinomas, follicular forms, five cases were papillary carcinomas - formed with tall-cells, three cases of papillary carcinomas, solid variants and one case was interpreted as an oncocytic variant. Most papillary carcinomas have been typically characterized by the presence of neoplastic papillae, composed of a central axis of fibro-vascular, branched, and coated by one or more layers of cubic or prismatic epithelial cells. In three typical papillary carcinomas, the stroma was composed of coarse connective axes rich in collagen fibers. The predominantly fibrous stroma, consisting of connective septum was observed in four cases, while in one case of papillary carcinoma, solid variant, we have identified a hyaline stroma; in one case was revealed a myxoid stroma. Diffuse stromal calcifications have been identified in two cases only. In the tumoral stroma, there were identified inflammatory infiltrates in nine cases, formed mostly of lymphocytes, and in one case, there was observed the presence of aggregated lymphoid nodules. The immunostaining with anti-CD34 antibody showed that in papillary thyroid carcinomas there is a well-represented vascularity, mostly made of small vessels (arterioles, venules, capillaries) with diameters between 7 and 50 µm, and immunostaining with anti-vimentin and anti-α-SMA antibody showed an increased number of fibroblasts, respectively myofibroblasts in the tumoral stroma. We believe that in the same thyroid tumor there are several clones of neoplastic cells that reshape the stroma, giving it certain histopathological characteristics.
[Mh] Termos MeSH primário: Carcinoma/patologia
Imuno-Histoquímica
Neoplasias da Glândula Tireoide/patologia
[Mh] Termos MeSH secundário: Carcinoma Papilar
Agregação Celular
Proliferação Celular
Seres Humanos
Cartilagem Hialina/patologia
Inflamação/patologia
Miofibroblastos/patologia
Células Estromais/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161112
[St] Status:MEDLINE


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[PMID]:27571686
[Au] Autor:Armakolas N; Dimakakos A; Armakolas A; Antonopoulos A; Koutsilieris M
[Ad] Endereço:Third Orthopaedic Clinic, KAT General Hospital, 14388 Athens, Greece.
[Ti] Título:Possible role of the Ec peptide of IGF­1Ec in cartilage repair.
[So] Source:Mol Med Rep;14(4):3066-72, 2016 Oct.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:The Ec peptide (PEc) of insulin-like growth factor 1 Ec (IGF-1Ec) induces human mesenchymal stem cell (hMSC) mobilization and activates extracellular signal­regulated kinase 1/2 (ERK 1/2) in various cells. The aim of the present study was to examine the effects of PEc on the mobilization and differentiation of hMSCs, as well as the possibility of its implementation in combination with transforming growth factor ß1 (TGF­ß1) for cartilage repair. The effects of the exogenous administration of PEc and TGF­ß1, alone and in combination, on hMSCs were assessed using a trypan blue assay, reverse transcription-quantitative polymerase chain reaction, western blot analysis, Alcian blue staining, wound healing assays and migration/invasion assays. It was determined that PEc is involved in the differentiation process of hMSCs towards hyaline cartilage. Treatment of hMSCs with either PEc, TGF­ß1 or both, demonstrated comparable cartilage matrix deposition. Furthermore, treatment with PEc in combination with TGF­ß1 was associated with a significant increase in hMSC mobilization when compared with treatment with TGF­ß1 or PEc alone (P<0.05). Thus, PEc appears to facilitate in vitro hMSC mobilization and differentiation towards chondrocytes, enhancing the role of TGF­ß1.
[Mh] Termos MeSH primário: Condrogênese/efeitos dos fármacos
Cartilagem Hialina/efeitos dos fármacos
Fator de Crescimento Insulin-Like I/farmacologia
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/efeitos dos fármacos
Peptídeos/farmacologia
[Mh] Termos MeSH secundário: Adulto
Proliferação Celular/efeitos dos fármacos
Separação Celular
Células Cultivadas
Colágeno/análise
Seres Humanos
Cartilagem Hialina/citologia
Fator de Crescimento Transformador beta1/farmacologia
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptides); 0 (Transforming Growth Factor beta1); 67763-96-6 (Insulin-Like Growth Factor I); 9007-34-5 (Collagen)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5627


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[PMID]:27562858
[Au] Autor:Noorafshan A; Niazi B; Mohamadpour M; Hoseini L; Hoseini N; Owji AA; Rafati A; Sadeghi Y; Karbalay-Doust S
[Ad] Endereço:Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
[Ti] Título:First and second order stereology of hyaline cartilage: Application on mice femoral cartilage.
[So] Source:Ann Anat;208:24-30, 2016 Nov.
[Is] ISSN:1618-0402
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Stereological techniques could be considered in research on cartilage to obtain quantitative data. The present study aimed to explain application of the first- and second-order stereological methods on articular cartilage of mice and the methods applied on the mice exposed to cadmium (Cd). The distal femoral articular cartilage of BALB/c mice (control and Cd-treated) was removed. Then, volume and surface area of the cartilage and number of chondrocytes were estimated using Cavalieri and optical dissector techniques on isotropic uniform random sections. Pair-correlation function [g(r)] and cross-correlation function were calculated to express the spatial arrangement of chondrocytes-chondrocytes and chondrocytes-matrix (chondrocyte clustering/dispersing), respectively. The mean±standard deviation of the cartilage volume, surface area, and thickness were 1.4±0.1mm , 26.2±5.4mm , and 52.8±6.7µm, respectively. Besides, the mean number of chondrocytes was 680±200 (×10 ). The cartilage volume, cartilage surface area, and number of chondrocytes were respectively reduced by 25%, 27%, and 27% in the Cd-treated mice in comparison to the control animals (p<0.03). Estimates of g(r) for the cells and matrix against the dipole distances, r, have been plotted. This plot showed that the chondrocytes and the matrix were neither dispersed nor clustered in the two study groups. Application of design-based stereological methods and also evaluation of spatial arrangement of the cartilage components carried potential advantages for investigating the cartilage in different joint conditions. Chondrocyte clustering/dispersing and cellularity can be evaluated in cartilage assessment in normal or abnormal situations.
[Mh] Termos MeSH primário: Cádmio/toxicidade
Condrócitos/patologia
Fêmur/patologia
Cartilagem Hialina/patologia
Imagem Tridimensional/métodos
[Mh] Termos MeSH secundário: Anatomia Transversal
Animais
Condrócitos/efeitos dos fármacos
Fêmur/efeitos dos fármacos
Cartilagem Hialina/efeitos dos fármacos
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Modelos Anatômicos
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
00BH33GNGH (Cadmium)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160827
[St] Status:MEDLINE


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[PMID]:27465877
[Au] Autor:Lau TT; Zhang F; Tang W; Wang DA
[Ad] Endereço:School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457, Singapore.
[Ti] Título:Release of transgenic progranulin from a living hyaline cartilage graft model: An in vitro evaluation on anti-inflammation.
[So] Source:J Biomed Mater Res A;104(12):2968-2977, 2016 Dec.
[Is] ISSN:1552-4965
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Osteoarthritis (OA) is a prevalent condition that compromises and even jeopardizes the life quality of millions of people. Common symptoms in OA includes joint stiffness and soreness, and they are often associated with inflammations to various extend. Due to the avascular and aneural nature of articular hyaline cartilage, it has limited self-repair capabilities; especially under inflammatory conditions, damages inflicted on cartilage are often irreversible. Hence, treatment approaches focus on anti-inflammation or articular cartilage replacement. In this study, an engineered, dual-functional living hyaline cartilage graft (LhCG), capable of releasing transgenic anti-inflammatory cytokine-progranulin (PGRN) is developed and envisioned to simultaneously fulfil both requirements. The therapeutic functionality of PGRN releasing LhCG is evaluated by co-culturing the constructs with tumor necrosis factor-alpha (TNFα) secreting THP-1 cells to simulate the inflammatory condition in arthritis. Non-transgenic LhCG constructs and non-coculture sample groups were set up as controls. Gene expression and ECM composition changes across samples were assessed to understand the effects of PGRN as well as inflammatory environment on the cartilage graft. Collectively, the results in this study suggest that in situ release of transgenic recombinant PGRN protects LhCG from induced inflammation in vitro; contrastively, in the absence of PGRN, cartilage grafts are at risk of being degraded and mineralized under exposure to TNFα signaling. This shows that cartilage graft itself can be at risk of degradation or calcification when implanted in arthritic microenvironment. Hence, the inflammatory microenvironment has to be considered in cartilage replacement therapy to increase chances of successful joint mobility restoration. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2968-2977, 2016.
[Mh] Termos MeSH primário: Cartilagem Hialina/imunologia
Peptídeos e Proteínas de Sinalização Intercelular/imunologia
Osteoartrite/imunologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Células Cultivadas
Condrócitos/imunologia
Condrócitos/patologia
Técnicas de Cocultura
Seres Humanos
Cartilagem Hialina/patologia
Monócitos/imunologia
Monócitos/patologia
Osteoartrite/patologia
Proteínas Recombinantes/imunologia
Suínos
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (GRN protein, human); 0 (Intercellular Signaling Peptides and Proteins); 0 (Recombinant Proteins); 0 (TNF protein, human); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160729
[St] Status:MEDLINE
[do] DOI:10.1002/jbm.a.35845



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