Base de dados : MEDLINE
Pesquisa : A03.556.124.526.209 [Categoria DeCS]
Referências encontradas : 10760 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 1076 ir para página                         

  1 / 10760 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29458539
[Au] Autor:Ma ST; Ding GJ; Huang XW; Wang ZW; Wang L; Yu ML; Shi W; Jiang YP; Tang LJ; Xu YG; Li YJ
[Ad] Endereço:College of Veterinary Medicine, Northeast Agricultural University, Mu Cai Street No. 59, Xiang Fang District, Harbin, PR China.
[Ti] Título:Immunogenicity in chickens with orally administered recombinant chicken-borne Lactobacillus saerimneri expressing FimA and OmpC antigen of O78 avian pathogenic Escherichia coli.
[So] Source:J Med Microbiol;67(3):441-451, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Avian colibacillosis is responsible for economic losses to poultry producers worldwide. To combat this, we aimed to develop an effective oral vaccine for chicken against O78 avian pathogenic Escherichia coli (APEC) infection through a Lactobacillus delivery system. METHODOLOGY: Eight Lactobacillus strains isolated from the intestines of broiler chickens were evaluated based on their in vitro adherence ability to assess their potential as a delivery vector. Fimbrial subunit A (FimA) and outer-membrane protein C (OmpC) of APEC with and without fusion to dendritic cell-targeting peptide (DCpep) and microfold cell-targeting peptide (Co1) were displayed on the surface of Lactobacillus saerimneri M-11 and yielded vaccine groups (pPG-ompC-fimA/M-11 and pPG-ompC-fimA-Co1-DCpep/M-11, respectively). The colonization of the recombinant strains in vivo was assessed and the immunogenicity and protective efficacy of orally administered recombinant strains in chickens were evaluated. RESULTS: The colonization of the recombinant strains in vivo revealed no significant differences between the recombinant and wild-type strains. Chickens orally administered with vaccine groups showed significantly higher levels of OmpC/FimA-specific IgG in serum and mucosal IgA in cecum lavage, nasal lavage and stool compared to the pPG/M-11 group. After challenge with APEC CVCC1553, better protective efficacy was observed in chickens orally immunized with pPG-ompC-fimA/M-11 and pPG-ompC-fimA-Co1-DCpep/M-11, but no significant differences were observed between the two groups. CONCLUSIONS: Recombinant chicken-borne L. saerimneri M-11 showed good immunogenicity in chickens, suggesting that it may be a promising vaccine candidate against APEC infections. However, the activity of mammalian DCpep and Co1 was not significant in chickens.
[Mh] Termos MeSH primário: Infecções por Escherichia coli/veterinária
Vacinas contra Escherichia coli/imunologia
Proteínas de Fímbrias/imunologia
Imunogenicidade da Vacina
Lactobacillus/genética
Porinas/imunologia
Doenças das Aves Domésticas/imunologia
[Mh] Termos MeSH secundário: Administração Oral
Animais
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/genética
Antígenos de Bactérias/imunologia
Ceco/imunologia
Galinhas
Escherichia coli/imunologia
Escherichia coli/patogenicidade
Infecções por Escherichia coli/imunologia
Infecções por Escherichia coli/prevenção & controle
Proteínas de Fímbrias/genética
Imunoglobulina A/sangue
Imunoglobulina G/sangue
Intestinos/microbiologia
Lactobacillus/crescimento & desenvolvimento
Lactobacillus/imunologia
Lactobacillus/isolamento & purificação
Porinas/genética
Doenças das Aves Domésticas/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Escherichia coli Vaccines); 0 (Immunoglobulin A); 0 (Immunoglobulin G); 0 (OmpC protein); 0 (Porins); 0 (fimbrillin); 147680-16-8 (Fimbriae Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000679


  2 / 10760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29406051
[Au] Autor:Bansal R; Huang T; Chun S
[Ad] Endereço:Division of Gastroenterology and Hepatology, Icahn School of Medicine at Mount Sinai, Elmhurst Hospital Center, Elmhurst, New York. Electronic address: raghav.bansal@mountsinai.org.
[Ti] Título:Trichuriasis.
[So] Source:Am J Med Sci;355(2):e3, 2018 Feb.
[Is] ISSN:1538-2990
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Ceco
Tricuríase/patologia
Trichuris
[Mh] Termos MeSH secundário: Animais
Ceco/parasitologia
Ceco/patologia
Seres Humanos
Masculino
Meia-Idade
Tricuríase/tratamento farmacológico
[Pt] Tipo de publicação:CASE REPORTS; LETTER
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


  3 / 10760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29355546
[Au] Autor:Shen P; Zhang Z; He Y; Gu C; Zhu K; Li S; Li Y; Lu X; Liu J; Zhang N; Cao Y
[Ad] Endereço:College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
[Ti] Título:Magnolol treatment attenuates dextran sulphate sodium-induced murine experimental colitis by regulating inflammation and mucosal damage.
[So] Source:Life Sci;196:69-76, 2018 Mar 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Magnolol, the main and active ingredient of the Magnolia officinalis, has been widely used in traditional prescription to the human disorders. Magnolol has been proved to have several pharmacological properties including anti-bacterial, anti-oxidant and anti-inflammatory activities. However, the effects of magnolol on ulcerative colitis (UC) have not been reported. The aim of this study was to investigate the protective effects and mechanisms of magnolol on dextran sulphate sodium (DSS)-induced colitis in mice. The results showed that magnolol significantly alleviated DSS-induced body weight loss, disease activities index (DAI), colon length shortening and colonic pathological damage. In addition, magnolol restrained the expression of TNF-α, IL-1ß and IL-12 via the regulation of nuclear factor-κB (NF-κB) and Peroxisome proliferator-activated receptor-γ (PPAR-γ) pathways. Magnolol also enhanced the expression of ZO-1 and occludin in DSS-induced mice colonic tissues. These results showed that magnolol played protective effects on DSS-induced colitis and may be an alternative therapeutic reagent for colitis treatment.
[Mh] Termos MeSH primário: Compostos de Bifenilo/uso terapêutico
Colite Ulcerativa/induzido quimicamente
Colite Ulcerativa/tratamento farmacológico
Sulfato de Dextrana
Fármacos Gastrointestinais/uso terapêutico
Mucosa Intestinal/efeitos dos fármacos
Lignanas/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Ceco/microbiologia
Colite Ulcerativa/patologia
Colo/patologia
Citocinas/biossíntese
Inflamação/fisiopatologia
Inflamação/prevenção & controle
Mediadores da Inflamação
Mucosa Intestinal/patologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Ocludina/antagonistas & inibidores
Ocludina/biossíntese
PPAR gama/efeitos dos fármacos
Perda de Peso/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Cytokines); 0 (Gastrointestinal Agents); 0 (Inflammation Mediators); 0 (Lignans); 0 (Occludin); 0 (PPAR gamma); 001E35HGVF (magnolol); 9042-14-2 (Dextran Sulfate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180123
[St] Status:MEDLINE


  4 / 10760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29330048
[Au] Autor:Watabe T; Nagaishi T; Tsugawa N; Kojima Y; Jose N; Hosoya A; Onizawa M; Nemoto Y; Oshima S; Nakamura T; Karasuyama H; Adachi T; Watanabe M
[Ad] Endereço:Department of Gastroenterology, Graduate School of Medical Science, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
[Ti] Título:B cell activation in the cecal patches during the development of an experimental colitis model.
[So] Source:Biochem Biophys Res Commun;496(2):367-373, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although previous studies have suggested that appendix seems to be involved in the colitis, the role of this in the pathogenesis remains unclear. In this study, we assessed the importance of appendiceal lymphoid follicles, specifically the cecal patches (CP) in mice, using an experimental colitis model. Treatment with oxazolone resulted in ulcerations particularly at CP with follicular expansion as well as colitis. The colitis was attenuated by either appendectomy or the absence of mature B cells. We therefore established an intravital imaging system accompanied by the fluorescence resonance energy transfer technology to analyze the dynamic immune response of CP B cells. Our observation revealed frequent Ca signaling in CP B cells during the early phase of colitis development. These findings suggested that the CP B cells may be involved in the pathogenesis of colitis including inflammatory bowel diseases in humans.
[Mh] Termos MeSH primário: Apêndice/imunologia
Ceco/imunologia
Colite/imunologia
Colo/imunologia
Estruturas Linfoides Terciárias/imunologia
[Mh] Termos MeSH secundário: Animais
Apêndice/diagnóstico por imagem
Apêndice/patologia
Linfócitos B/imunologia
Linfócitos B/patologia
Sinalização do Cálcio
Ceco/diagnóstico por imagem
Ceco/patologia
Colite/induzido quimicamente
Colite/diagnóstico por imagem
Colite/patologia
Colo/diagnóstico por imagem
Colo/patologia
Modelos Animais de Doenças
Seres Humanos
Microscopia Intravital
Ativação Linfocitária
Camundongos
Camundongos Endogâmicos C57BL
Oxazolona
Estruturas Linfoides Terciárias/diagnóstico por imagem
Estruturas Linfoides Terciárias/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
15646-46-5 (Oxazolone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180114
[St] Status:MEDLINE


  5 / 10760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28458349
[Au] Autor:Matsutani T; Tamura K; Kutsukake M; Matsuda A; Tachikawa E; Uchida E
[Ad] Endereço:Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Nippon Medical School.
[Ti] Título:Impact of Pioglitazone on Macrophage Dynamics in Adipose Tissues of Cecal Ligation and Puncture-Treated Mice.
[So] Source:Biol Pharm Bull;40(5):638-644, 2017.
[Is] ISSN:1347-5215
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Pioglitazone improves sepsis-induced organ injury accompanied with anti-inflammatory effects on visceral adipose tissue. However, its action in adipose immune cells remains to be ascertained. We investigated the effects of pioglitazone on visceral adipose macrophage population and polarisation in cecal ligation and puncture (CLP)-induced sepsis mice. Eight-week-old male mice were assigned to 3 groups: 1) sham-operated group, 2) CLP group, or 3) pioglitazone-treated CLP group. Pioglitazone (10 mg/kg) was injected intraperitonally for 7 d and CLP surgery was performed. Visceral adipose tissues were collected 24 h after the surgery. mRNA expression of several macrophage markers (inducible nitric oxide synthase (iNOS) for M1, arginase1 (Arg1) and interleukin (IL)-10 for M2, CD163 and F4/80 for mature macrophages) and inflammatory adipokines (IL-6, monocyte chemoattractant protein-1: MCP-1) was quantified by real-time RT-PCR. Tissue sections were subjected to the immunohistochemical analysis and the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay. CLP significantly enhanced Arg1, IL-10 and iNOS mRNA expressions as compared with the sham group, and pioglitazone significantly increased the mRNA level of CD163 and F4/80 in CLP mice. Expression of IL-6 and MCP-1 stimulated by CLP was reduced by pioglitazone treatment. Increased CD11b/c- and CD163-positive cells as well as apoptotic cells were observed in the CLP group and the pioglitazone-treated group. The data indicate that M1/M2 macrophage activation of visceral adipose tissues is induced in CLP-induced mice, and the function of macrophages recruited from surrounding organs may be modulated by pioglitazone treatment.
[Mh] Termos MeSH primário: Hipoglicemiantes/farmacologia
Gordura Intra-Abdominal/patologia
Macrófagos/efeitos dos fármacos
Sepse/patologia
Tiazolidinedionas/farmacologia
[Mh] Termos MeSH secundário: Adipocinas/metabolismo
Animais
Arginase/análise
Biomarcadores/análise
Ceco
Quimiocina CCL2/metabolismo
Hipoglicemiantes/administração & dosagem
Injeções Intraperitoneais
Interleucina-10/sangue
Ligadura
Masculino
Camundongos
Punções
Tiazolidinedionas/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adipokines); 0 (Biomarkers); 0 (Ccl2 protein, mouse); 0 (Chemokine CCL2); 0 (Hypoglycemic Agents); 0 (IL10 protein, mouse); 0 (Thiazolidinediones); 130068-27-8 (Interleukin-10); EC 3.5.3.1 (Arg1 protein, mouse); EC 3.5.3.1 (Arginase); X4OV71U42S (pioglitazone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1248/bpb.b16-00883


  6 / 10760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29304153
[Au] Autor:Barszcz M; Taciak M; Tusnio A; Skomial J
[Ad] Endereço:Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Jablonna, Poland.
[Ti] Título:Effects of dietary level of tannic acid and protein on internal organ weights and biochemical blood parameters of rats.
[So] Source:PLoS One;13(1):e0190769, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tannic acid (TA) is a polyphenolic compound with a health-promoting potential for humans. It is hypothesised that TA effects on the relative weight of internal organs and biochemical blood indices are modified by dietary protein level in rats. The study involved 72 rats divided into 12 groups fed diets with 10 or 18% of crude protein (CP) and supplemented with 0, 0.25, 0.5, 1, 1.5 or 2% of TA. After 3 weeks of feeding, the relative weight of the caecum was greater in rats fed TA diets, while feeding diets with 10% of CP increased the relative weight of the stomach, small intestine and caecum, but decreased that of kidneys and spleen. Albumin concentration was higher in rats fed 0.25% and 0.5% TA diets than in rats given the 2% TA diets. The 2% TA diets reduced creatine kinase (CK) activity compared to non-supplemented diets and those with 0.5, 1 and 1.5% of TA. Rats fed the 10% CP diets had a higher activity of alkaline phosphatase, amylase, and γ-glutamyltransferase as well as the concentration of iron and cholesterol, but lower that of urea and uric acid. The interaction affected only cholinesterase activity. In conclusion, TA induced caecal hypertrophy and could act as a cardioprotective agent, as demonstrated by reduced CK activity, but these effects were not modified by dietary protein level.
[Mh] Termos MeSH primário: Dieta
Proteínas na Dieta
Taninos
[Mh] Termos MeSH secundário: Animais
Ceco/anatomia & histologia
Colesterol/sangue
Colinesterases/sangue
Creatina Quinase/sangue
Intestino Delgado/anatomia & histologia
Rim/anatomia & histologia
Masculino
Tamanho do Órgão
Ratos Endogâmicos WF
Albumina Sérica
Baço/anatomia & histologia
Estômago/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dietary Proteins); 0 (Serum Albumin); 0 (Tannins); 97C5T2UQ7J (Cholesterol); EC 2.7.3.2 (Creatine Kinase); EC 3.1.1.8 (Cholinesterases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190769


  7 / 10760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28745637
[Au] Autor:Kochall S; Thepkaysone ML; García SA; Betzler AM; Weitz J; Reissfelder C; Schölch S
[Ad] Endereço:Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden.
[Ti] Título:Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer.
[So] Source:J Vis Exp;(125), 2017 Jul 18.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite the advantages of easy applicability and cost-effectiveness, subcutaneous mouse models have severe limitations and do not accurately simulate tumor biology and tumor cell dissemination. Orthotopic mouse models have been introduced to overcome these limitations; however, such models are technically demanding, especially in hollow organs such as the large bowel. In order to produce uniform tumors which reliably grow and metastasize, standardized techniques of tumor cell preparation and injection are critical. We have developed an orthotopic mouse model of colorectal cancer (CRC) which develops highly uniform tumors and can be used for tumor biology studies as well as therapeutic trials. Tumor cells from either primary tumors, 2-dimensional (2D) cell lines or 3-dimensional (3D) organoids are injected into the cecum and, depending on the metastatic potential of the injected tumor cells, form highly metastatic tumors. In addition, CTCs can be found regularly. We here describe the technique of tumor cell preparation from both 2D cell lines and 3D organoids as well as primary tumor tissue, the surgical and injection techniques as well as the isolation of CTCs from the tumor-bearing mice, and present tips for troubleshooting.
[Mh] Termos MeSH primário: Neoplasias Colorretais/patologia
Células Neoplásicas Circulantes/metabolismo
[Mh] Termos MeSH secundário: Animais
Ceco/patologia
Ceco/cirurgia
Neoplasias Colorretais/metabolismo
Modelos Animais de Doenças
Células HCT116
Seres Humanos
Neoplasias Hepáticas/patologia
Neoplasias Hepáticas/secundário
Neoplasias Pulmonares/patologia
Neoplasias Pulmonares/secundário
Camundongos
Camundongos Endogâmicos NOD
Transplante Heterólogo
Gravação em Vídeo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.3791/55357


  8 / 10760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29196049
[Au] Autor:Wu T; Yang L; Jiang J; Ni Y; Zhu J; Zheng X; Wang Q; Lu X; Fu Z
[Ad] Endereço:College of Biotechnology and Bioengineering, Zhejiang University of Technology, China.
[Ti] Título:Chronic glucocorticoid treatment induced circadian clock disorder leads to lipid metabolism and gut microbiota alterations in rats.
[So] Source:Life Sci;192:173-182, 2018 Jan 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIM: Glucocorticoids (GCs), steroid hormones synthetized by the adrenal gland, are regulated by circadian cycles, and dysregulation of GC signaling can lead to the development of metabolic syndrome. The effects and potential mechanism of GCs in physiology were investigated in the present study. MAIN METHODS: Male Wistar rats were orally administered dexamethasone sodium phosphate (DEX, 0.01 and 0.05mg/kg body weight per day) for 7weeks. KEY FINDING: DEX treatment attenuated body weight gain and reduced food intake, whereas it induced the accumulation of fat. Administration of DEX induced dysregulation of the expression of lipogenic genes in both fat and liver. Moreover, the mRNA levels of genes related to mitochondrial biogenesis and function were significantly downregulated in the liver and fat of DEX-treated rats. Furthermore, DEX treatment caused a significant reduction in the richness and diversity of the microbiota in the colon, as assessed using high-throughput sequencing of the 16s rRNA gene V3-V4 region, an increase in inflammatory cell infiltration, and a decrease in mucus secretion in the colon. Additionally, DEX administration induced phase shift or loss of circadian rhythmicity of clock-related genes in peripheral tissues. These results were associated with higher serum corticosterone levels and upregulation of GC receptor (GR) expression in peripheral tissues. SIGNIFICANCE: Our findings indicate that long-term administration of GC caused lipid accumulation, changes in the structure of the intestinal flora, and reduced colonic mucus secretion in vivo. The mechanism of these physiological changes may involve a circadian rhythm disorder and dysregulation of GR expression.
[Mh] Termos MeSH primário: Relógios Circadianos/efeitos dos fármacos
Dexametasona/farmacologia
Microbioma Gastrointestinal/efeitos dos fármacos
Metabolismo dos Lipídeos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Tecido Adiposo/efeitos dos fármacos
Tecido Adiposo/metabolismo
Animais
Ceco/efeitos dos fármacos
Ceco/microbiologia
Colo/efeitos dos fármacos
Colo/microbiologia
Colo/secreção
Corticosterona/sangue
Ingestão de Alimentos/efeitos dos fármacos
Teste de Tolerância a Glucose
Glicolipídeos/metabolismo
Metabolismo dos Lipídeos/genética
Fígado/efeitos dos fármacos
Fígado/metabolismo
Masculino
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Ratos
Ratos Wistar
Receptores de Glucocorticoides/biossíntese
Ganho de Peso/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycolipids); 0 (Receptors, Glucocorticoid); 7S5I7G3JQL (Dexamethasone); W980KJ009P (Corticosterone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE


  9 / 10760 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28470335
[Au] Autor:Han GG; Song AA; Kim EB; Yoon SH; Bok JD; Cho CS; Kil DY; Kang SK; Choi YJ
[Ad] Endereço:Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
[Ti] Título:Improved antimicrobial activity of Pediococcus acidilactici against Salmonella Gallinarum by UV mutagenesis and genome shuffling.
[So] Source:Appl Microbiol Biotechnol;101(13):5353-5363, 2017 Jul.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Pediococcus acidilactici is a widely used probiotic, and Salmonella enterica serovar Gallinarum (SG) is a significant pathogen in the poultry industry. In this study, we improved the antimicrobial activity of P. acidilactici against SG using UV mutation and genome shuffling (GS). To improve antimicrobial activity against SG, UV mutagenesis was performed against wild-type P. acidilactici (WT), and five mutants showed improved antimicrobial activity. To further improve antimicrobial activity, GS was performed on five UV mutants. Following GS, four mutants showed improved antimicrobial activity compared with the UV mutants and WT. The antimicrobial activity of GS1 was highest among the mutants; however, the activity was reduced when the culture supernatant was treated with proteinase K, suggesting that the improved antimicrobial activity is due to a proteinous substance such as bacteriocin. To validate the activity of GS1 in vivo, we designed multi-species probiotics and performed broiler feeding experiments. Groups consisted of no treatment (NC), avilamycin-treated (PC), probiotic group 1 containing WT (T1), and probiotic group 2 containing GS1 (T2). In broiler feeding experiments, coliform bacteria were significantly reduced in T2 compared with NC, PC, and T1. The cecal microbiota was modulated and pathogenic bacteria were reduced by GS1 oral administration. In this study, GS1 showed improved antimicrobial activity against SG in vitro and reduced pathogenic bacteria in a broiler feeding experiment. These results suggest that GS1 can serve as an efficient probiotic, as an alternative to antibiotics in the poultry industry.
[Mh] Termos MeSH primário: Antibiose
Embaralhamento de DNA
Mutagênese
Pediococcus acidilactici/genética
Pediococcus acidilactici/fisiologia
Probióticos
Salmonella/fisiologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Anti-Infecciosos
Bacteriocinas/biossíntese
Bacteriocinas/farmacologia
Ceco/microbiologia
Galinhas/microbiologia
Meios de Cultura/química
Endopeptidase K/metabolismo
Genoma Bacteriano
Pediococcus acidilactici/efeitos dos fármacos
Pediococcus acidilactici/efeitos da radiação
Doenças das Aves Domésticas/microbiologia
Doenças das Aves Domésticas/terapia
Probióticos/química
Salmonella/efeitos dos fármacos
Salmonelose Animal/microbiologia
Salmonelose Animal/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Bacteriocins); 0 (Culture Media); EC 3.4.21.64 (Endopeptidase K)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-017-8293-6


  10 / 10760 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28455617
[Au] Autor:Peng Y; Yu K; Mu C; Hang S; Che L; Zhu W
[Ad] Endereço:Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, People's Republic of China.
[Ti] Título:Progressive response of large intestinal bacterial community and fermentation to the stepwise decrease of dietary crude protein level in growing pigs.
[So] Source:Appl Microbiol Biotechnol;101(13):5415-5426, 2017 Jul.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The study aimed to determine the effects of reduction of dietary crude protein (CP) level with balanced essential amino acids (EAA) on intestinal bacteria and their metabolites of growing pigs. Forty pigs (initial BW 13.50 ± 0.50 kg, 45 ± 2 days of age) were randomly assigned to four dietary treatments containing CP levels at 20.00% (normal crude protein, NP); 17.16% (medium crude protein, MP); 15.30% (low crude protein, LP); and 13.90% (extremely low crude protein, ELP), respectively. Crystalline AAs were added to meet the EAA requirement of pigs. After 4-week feeding, eight pigs per treatment (n = 8) were randomly selected and slaughtered for sampling of ileal, cecal, and colonic digesta and mucosa. Pigs with moderately reduced CP level had increased bacterial diversity, with the Shannon diversity indices for the colon digesta in the LP group and mucosa in the MP and LP groups significantly (P < 0.05) higher than those in the NP and ELP groups. As the CP level reduces, the Bifidobacterium population were linearly decreased (P < 0.05) both in ileum, cecum, and colon, and the ELP group had the lowest Bifidobacterium population in the cecum and colon, with its value significantly lower than NP and MP groups (P < 0.05). However, the ELP group had the highest population of Escherichia coli in the colon, with its value significantly higher than the LP group (P < 0.05). For bacterial metabolites, as CP level decreased, total short-chain fatty acid (T-SCFA), acetate, and butyrate were linearly increased (linear, P < 0.05) in the ileum, while all SCFAs except formate in the cecum and T-SCFA and acetate in the colon, were linearly decreased (P < 0.05). Reducing CP level led to a linear decrease of microbial crude protein (MCP) in the ileum (P < 0.05) and ammonia in all intestine segments (P < 0.05). The spermidine in cecum and total amines, cadaverine, methylamine, and spermidine in colon were shown a quadratic change (P < 0.05) as dietary CP decreases, with the highest concentration in LP group. These findings suggest that moderate reduction of dietary CP level may benefit large intestinal bacterial community and its fermentation, which was negatively affected by extremely low CP diet.
[Mh] Termos MeSH primário: Aminoácidos Essenciais/administração & dosagem
Ração Animal
Ceco/microbiologia
Colo/microbiologia
Proteínas na Dieta/administração & dosagem
Fermentação
Consórcios Microbianos/fisiologia
[Mh] Termos MeSH secundário: Aminas/análise
Aminoácidos Essenciais/análise
Ração Animal/análise
Animais
Bifidobacterium/isolamento & purificação
Proteínas na Dieta/análise
Proteínas na Dieta/química
Digestão
Escherichia coli/isolamento & purificação
Ácidos Graxos Voláteis/metabolismo
Íleo/microbiologia
Distribuição Aleatória
Espermidina/análise
Suínos
Desmame
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amines); 0 (Amino Acids, Essential); 0 (Dietary Proteins); 0 (Fatty Acids, Volatile); U87FK77H25 (Spermidine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-017-8285-6



página 1 de 1076 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde