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[PMID]:29352311
[Au] Autor:Bonnet-Serrano F; Diedisheim M; Mallone R; Larger E
[Ad] Endereço:Assistance Publique Hôpitaux de Paris, Hôpital Cochin, UF d'Hormonologie, DHU « AUTHORS ¼, Paris, France.
[Ti] Título:Decreased α-cell mass and early structural alterations of the exocrine pancreas in patients with type 1 diabetes: An analysis based on the nPOD repository.
[So] Source:PLoS One;13(1):e0191528, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: Abnormal glucagon secretion and functional alterations of the exocrine pancreas have been described in patients with type 1 diabetes (T1D), but their respective anatomical substrata have seldom been investigated. Our aim was to develop an automated morphometric analysis process to characterize the anatomy of α-cell and exocrine pancreas in patients with T1D, using the publicly available slides of the Network for Pancreatic Organ Donors (nPOD). MATERIALS AND METHODS: The ratio of ß- and α-cell area to total tissue area were quantified in 75 patients with T1D (thereafter patients) and 66 control subjects (thereafter controls), on 2 insulin-stained and 4 glucagon-stained slides from both the head and the tail of the pancreas. The ß- and α-cell masses were calculated in the 66 patients and the 50 controls for which the pancreas weight was available. Non-exocrine-non-endocrine tissue area (i.e. non-acinar, non-insular tissue) to total tissue area ratio was evaluated on both insulin- and glucagon-stained slides. Results were expressed as mean ±SD. RESULTS: An automated quantification method was set up using the R software and was validated by quantification of ß-cell mass, a well characterized parameter. ß-cell mass was 29.6±112 mg in patients and 628 ±717 mg in controls (p<0.0001). α-cell mass was 181±176 mg in patients and 349 ±241mg in controls (p<0.0001). Non-exocrine-non-endocrine area to total tissue area ratio was 39±9% in patients and 29± 10% in controls (p<0.0001) and increased with age in both groups, with no correlation with diabetes duration in patients. CONCLUSION: The absolute α-cell mass was lower in patients compared to controls, in proportion to the decrease in pancreas weight observed in patients. Non-exocrine-non-endocrine area to total tissue area ratio increased with age in both groups but was higher in patients at all ages.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 1/patologia
Células Secretoras de Glucagon/patologia
Pâncreas Exócrino/patologia
[Mh] Termos MeSH secundário: Tecido Adiposo/patologia
Adolescente
Adulto
Estudos de Casos e Controles
Criança
Diabetes Mellitus Tipo 1/fisiopatologia
Feminino
Fibrose
Seres Humanos
Interpretação de Imagem Assistida por Computador
Células Secretoras de Insulina/patologia
Masculino
Meia-Idade
Tamanho do Órgão
Doadores de Tecidos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180121
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191528


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[PMID]:29095290
[Au] Autor:Wang H; Xiu D; Tao M
[Ad] Endereço:Department of General Surgery, Peking University Third Hospital, Haidian District, Beijing, P.R. China.
[Ti] Título:The pancreatic juice length in the stent tube as the predicting factor of clinical relevant postoperative pancreatic fistula after pancreaticoduodenectomy.
[So] Source:Medicine (Baltimore);96(44):e8451, 2017 Nov.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Several risk factors for pancreatic fistula had been widely reported, but there was no research focusing on the exocrine output of remnant gland.During the study period of January 2015 to September 2016, 82 patients accepted pancreaticoduodenectomy (PD, end-to-end dunking pancreaticojejunostomy with internal stent tube). All the data were collected, including preoperative medical status, operative course, final pathology, gland texture, pancreatic duct diameter, size of the stent, length of pancreatic juice in the stent tube, width of the pancreatic stump, diameter of the jejunum and the status of postoperative pancreatic fistula (POPF). POPF was defined according to International Study Group of Pancreatic Fistula criteria.The diameter of pancreatic duct in the POPF group was significantly smaller than that in the group without POPF (1.99 vs 2.90 mm, P = .000). The length of pancreatic juice in the stent tube in the POPF group was significantly longer than that in the group without POPF (18.04 vs 6.92 cm, P = .014). There were more pancreatic ductal adenocarcinoma cases and hard glands in the group without POPF. The length of pancreatic juice in the clinically relevant postoperative pancreatic fistula (CR-POPF) group was significantly longer than that in the grade A group (32.4 vs 9.21 cm, P = .000). Multivariate analysis identified gland texture and length of pancreatic juice as independent predictors for pancreatic fistula. Multivariate analysis also identified the length of pancreatic juice as an independent predictor for CR-POPF.The length of pancreatic juice in the stent tube might be a useful predictive factor of POPF after PD, especially for CR-POPF.
[Mh] Termos MeSH primário: Fístula Pancreática/etiologia
Suco Pancreático/secreção
Pancreaticoduodenectomia/instrumentação
Complicações Pós-Operatórias/etiologia
Stents/efeitos adversos
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Carcinoma Ductal Pancreático/cirurgia
Feminino
Seres Humanos
Jejuno/patologia
Jejuno/cirurgia
Masculino
Meia-Idade
Análise Multivariada
Pâncreas/patologia
Pâncreas/cirurgia
Pâncreas Exócrino/secreção
Pâncreas Exócrino/cirurgia
Neoplasias Pancreáticas/cirurgia
Pancreaticoduodenectomia/efeitos adversos
Pancreaticoduodenectomia/métodos
Pancreaticojejunostomia/efeitos adversos
Pancreaticojejunostomia/instrumentação
Pancreaticojejunostomia/métodos
Período Pós-Operatório
Estudos Retrospectivos
Fatores de Risco
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171103
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008451


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[PMID]:28693787
[Au] Autor:Trout AT; Wallihan DB; Serai S; Abu-El-Haija M
[Ad] Endereço:Department of Radiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH. Electronic address: andrew.trout@cchmc.org.
[Ti] Título:Secretin-Enhanced Magnetic Resonance Cholangiopancreatography for Assessing Pancreatic Secretory Function in Children.
[So] Source:J Pediatr;188:186-191, 2017 Sep.
[Is] ISSN:1097-6833
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To assess the accuracy and interrater reproducibility of measurements of pancreatic secretory function by magnetic resonance cholangiopancreatography in response to secretin administration and to describe our experience using the technique to noninvasively assess pancreatic secretory function in a pediatric population. STUDY DESIGN: In the accuracy study, phantoms with varying fluid volume (47-206 mL) were imaged using the clinical quantification sequence. Fluid volume was measured by image segmentation (ImageJ). Measurement accuracy was expressed in terms of error (absolute and percent) relative to known fluid volume. In the reproducibility study and clinical experience, 31 patients with suspected pancreatic disease underwent 33 secretin-enhanced magnetic resonance cholangiopancreatography exams. Two-dimensional T2-weighted, fat-saturated single shot fast spin echo sequences were acquired before and after secretin injection (0.2 µg/kg, max 16 µg). Secreted fluid volume (postsecretin minus presecretin) was independently measured by 2 blinded reviewers. Between reviewer measurement reproducibility was assessed based on correlation (Spearman) and bias (Bland-Altman analysis). RESULTS: For the accuracy study, fluid volumes were measured with mean volume errors of -0.3 to +12.5 mL (percent error -0.03% to +9.0%). For the reproducibility study, the mean secreted fluid volumes measured by reviewer 1 and reviewer 2 were 79.1 ± 54.3 mL (range 5.5-215.4) and 77.2 ± 47.1 mL (range 6.7-198.1 mL), respectively. Measured secreted fluid volumes were very strongly correlated (r = 0.922) between reviewers with a bias of only 1.9 mL (95% limits of agreement -40.5 to 44.2). CONCLUSIONS: Measurement of fluid volume by magnetic resonance imaging is highly accurate with <10% (<13 mL) error in measured volume. Measurements of pancreatic secreted fluid volume in response to secretin by magnetic resonance cholangiopancreatography are highly reproducible with a bias of <2 mL between reviewers.
[Mh] Termos MeSH primário: Colangiopancreatografia por Ressonância Magnética
Insuficiência Pancreática Exócrina/diagnóstico
Testes de Função Pancreática
Secretina/farmacocinética
[Mh] Termos MeSH secundário: Adolescente
Biomarcadores/análise
Criança
Seres Humanos
Pâncreas Exócrino/metabolismo
Pancreatite Crônica/etiologia
Imagens de Fantasmas
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 1393-25-5 (Secretin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE


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[PMID]:28639695
[Au] Autor:Ding L; Liou GY; Schmitt DM; Storz P; Zhang JS; Billadeau DD
[Ad] Endereço:Division of Oncology Research and Schulze Center for Novel Therapeutics, Mayo Clinic, Rochester, MN, USA.
[Ti] Título:Glycogen synthase kinase-3ß ablation limits pancreatitis-induced acinar-to-ductal metaplasia.
[So] Source:J Pathol;243(1):65-77, 2017 Sep.
[Is] ISSN:1096-9896
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Acinar-to-ductal metaplasia (ADM) is a reversible epithelial transdifferentiation process that occurs in the pancreas in response to acute inflammation. ADM can rapidly progress towards pre-malignant pancreatic intraepithelial neoplasia (PanIN) lesions in the presence of mutant KRas and ultimately pancreatic adenocarcinoma (PDAC). In the present work, we elucidate the role and related mechanism of glycogen synthase kinase-3beta (GSK-3ß) in ADM development using in vitro 3D cultures and genetically engineered mouse models. We show that GSK-3ß promotes TGF-α-induced ADM in 3D cultured primary acinar cells, whereas deletion of GSK-3ß attenuates caerulein-induced ADM formation and PanIN progression in Kras transgenic mice. Furthermore, we demonstrate that GSK-3ß ablation influences ADM formation and PanIN progression by suppressing oncogenic KRas-driven cell proliferation. Mechanistically, we show that GSK-3ß regulates proliferation by increasing the activation of S6 kinase. Taken together, these results indicate that GSK-3ß participates in early pancreatitis-induced ADM and thus could be a target for the treatment of chronic pancreatitis and the prevention of PDAC progression. Copyright © 2017 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Células Acinares/enzimologia
Carcinoma in Situ/prevenção & controle
Transdiferenciação Celular
Glicogênio Sintase Quinase 3 beta/deficiência
Pâncreas Exócrino/enzimologia
Ductos Pancreáticos/enzimologia
Neoplasias Pancreáticas/prevenção & controle
Pancreatite/enzimologia
[Mh] Termos MeSH secundário: Células Acinares/efeitos dos fármacos
Células Acinares/patologia
Animais
Carcinoma in Situ/enzimologia
Carcinoma in Situ/genética
Carcinoma in Situ/patologia
Proliferação Celular
Transdiferenciação Celular/efeitos dos fármacos
Transformação Celular Neoplásica/genética
Transformação Celular Neoplásica/metabolismo
Transformação Celular Neoplásica/patologia
Células Cultivadas
Ceruletídeo
Modelos Animais de Doenças
Progressão da Doença
Predisposição Genética para Doença
Glicogênio Sintase Quinase 3 beta/genética
Proteínas de Homeodomínio/genética
Masculino
Metaplasia
Camundongos Knockout
Pâncreas Exócrino/efeitos dos fármacos
Pâncreas Exócrino/patologia
Ductos Pancreáticos/efeitos dos fármacos
Ductos Pancreáticos/patologia
Neoplasias Pancreáticas/enzimologia
Neoplasias Pancreáticas/genética
Neoplasias Pancreáticas/patologia
Pancreatite/induzido quimicamente
Pancreatite/genética
Pancreatite/patologia
Fenótipo
Proteínas Proto-Oncogênicas p21(ras)/genética
Proteínas Quinases S6 Ribossômicas/metabolismo
Transdução de Sinais
Serina-Treonina Quinases TOR/metabolismo
Fatores de Tempo
Transativadores/genética
Fator de Necrose Tumoral alfa/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Homeodomain Proteins); 0 (Trans-Activators); 0 (Tumor Necrosis Factor-alpha); 0 (pancreatic and duodenal homeobox 1 protein); 888Y08971B (Ceruletide); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.1.1 (mTOR protein, mouse); EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta); EC 2.7.11.1 (Gsk3b protein, mouse); EC 2.7.11.1 (Ribosomal Protein S6 Kinases); EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1002/path.4928


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[PMID]:28628651
[Au] Autor:Lundberg M; Lindqvist A; Wierup N; Krogvold L; Dahl-Jørgensen K; Skog O
[Ad] Endereço:Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.
[Ti] Título:The density of parasympathetic axons is reduced in the exocrine pancreas of individuals recently diagnosed with type 1 diabetes.
[So] Source:PLoS One;12(6):e0179911, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To elucidate the etiology of type 1 diabetes, the affected pancreas needs to be thoroughly characterized. Pancreatic innervation has been suggested to be involved in the pathology of the disease and a reduction of sympathetic innervation of the islets was recently reported. In the present study, we hypothesized that parasympathetic innervation would be altered in the type 1 diabetes pancreas. Human pancreatic specimens were obtained from a unique cohort of individuals with recent onset or long standing type 1 diabetes. Density of parasympathetic axons was assessed by immunofluorescence and morphometry. Our main finding was a reduced density of parasympathetic axons in the exocrine, but not endocrine compartment of the pancreas in individuals with recent onset type 1 diabetes. The reduced density of parasympathetic axons in the exocrine compartment could have functional implications, e.g. be related to the exocrine insufficiency reported in type 1 diabetes patients. Further studies are needed to understand whether reduced parasympathetic innervation is a cause or consequence of type 1 diabetes.
[Mh] Termos MeSH primário: Axônios/fisiologia
Diabetes Mellitus Tipo 1/diagnóstico
Pâncreas Exócrino/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Estudos de Casos e Controles
Diabetes Mellitus Tipo 1/patologia
Feminino
Seres Humanos
Ilhotas Pancreáticas/metabolismo
Masculino
Microscopia de Fluorescência
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179911


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[PMID]:28617859
[Au] Autor:Masini M; Marselli L; Himpe E; Martino L; Bugliani M; Suleiman M; Boggi U; Filipponi F; Occhipinti M; Bouwens L; De Tata V; Marchetti P
[Ad] Endereço:Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.
[Ti] Título:Co-localization of acinar markers and insulin in pancreatic cells of subjects with type 2 diabetes.
[So] Source:PLoS One;12(6):e0179398, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To search for clues suggesting that beta cells may generate by transdifferentiation in humans, we assessed the presence of cells double positive for exocrine (amylase, carboxypeptidase A) and endocrine (insulin) markers in the pancreas of non-diabetic individuals (ND) and patients with type 2 diabetes (T2D). Samples from twelve ND and twelve matched T2D multiorgan donors were studied by electron microscopy, including amylase and insulin immunogold labeling; carboxypeptidase A immunofluorescence light microscopy assessment was also performed. In the pancreas from four T2D donors, cells containing both zymogen-like and insulin-like granules were observed, scattered in the exocrine compartment. Nature of granules was confirmed by immunogold labeling for amylase and insulin. Double positive cells ranged from 0.82 to 1.74 per mm2, corresponding to 0.26±0.045% of the counted exocrine cells. Intriguingly, cells of the innate immune systems (mast cells and/or macrophages) were adjacent to 33.3±13.6% of these hybrid cells. No cells showing co-localization of amylase and insulin were found in ND samples by electron microscopy. Similarly, cells containing both carboxypeptidase A and insulin were more frequently observed in the diabetic pancreata. These results demonstrate more abundant presence of cells containing both acinar markers and insulin in the pancreas of T2D subjects, which suggests possible conversion from one cellular type to the other and specific association with the diseased condition.
[Mh] Termos MeSH primário: Diabetes Mellitus Tipo 2/metabolismo
Insulina/metabolismo
Pâncreas Exócrino/metabolismo
[Mh] Termos MeSH secundário: Idoso
Biomarcadores/metabolismo
Diabetes Mellitus Tipo 2/patologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Pâncreas Exócrino/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Insulin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179398


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[PMID]:28410364
[Au] Autor:Pearring JN; San Agustin JT; Lobanova ES; Gabriel CJ; Lieu EC; Monis WJ; Stuck MW; Strittmatter L; Jaber SM; Arshavsky VY; Pazour GJ
[Ad] Endereço:Department of Ophthalmology, Duke University School of Medicine, Durham, North Carolina, United States of America.
[Ti] Título:Loss of Arf4 causes severe degeneration of the exocrine pancreas but not cystic kidney disease or retinal degeneration.
[So] Source:PLoS Genet;13(4):e1006740, 2017 Apr.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arf4 is proposed to be a critical regulator of membrane protein trafficking in early secretory pathway. More recently, Arf4 was also implicated in regulating ciliary trafficking, however, this has not been comprehensively tested in vivo. To directly address Arf4's role in ciliary transport, we deleted Arf4 specifically in either rod photoreceptor cells, kidney, or globally during the early postnatal period. Arf4 deletion in photoreceptors did not cause protein mislocalization or retinal degeneration, as expected if Arf4 played a role in protein transport to the ciliary outer segment. Likewise, Arf4 deletion in kidney did not cause cystic disease, as expected if Arf4 were involved in general ciliary trafficking. In contrast, global Arf4 deletion in the early postnatal period resulted in growth restriction, severe pancreatic degeneration and early death. These findings are consistent with Arf4 playing a critical role in endomembrane trafficking, particularly in the pancreas, but not in ciliary function.
[Mh] Termos MeSH primário: Fatores de Ribosilação do ADP/genética
Doenças Renais Císticas/genética
Pâncreas Exócrino/patologia
Degeneração Retiniana/genética
[Mh] Termos MeSH secundário: Animais
Cílios/genética
Cílios/patologia
Modelos Animais de Doenças
Seres Humanos
Rim/metabolismo
Rim/patologia
Doenças Renais Císticas/patologia
Camundongos
Camundongos Knockout
Motivos de Nucleotídeos/genética
Pâncreas Exócrino/crescimento & desenvolvimento
Células Fotorreceptoras/metabolismo
Células Fotorreceptoras/patologia
Degeneração Retiniana/patologia
Deleção de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.6.5.2 (ADP-Ribosylation Factors); EC 3.6.5.2 (ARF4 protein, mouse)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006740


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[PMID]:28348424
[Au] Autor:Dall'Aglio C; Polisca A; Cappai MG; Mercati F; Troisi A; Pirino C; Scocco P; Maranesi M
[Ad] Endereço:Department of Veterinary Medicine, University of Perugia. cecilia.dallaglio@unipg.it.
[Ti] Título:Immunohistochemistry detected and localized cannabinoid receptor type 2 in bovine fetal pancreas at late gestation.
[So] Source:Eur J Histochem;61(1):2761, 2017 Mar 07.
[Is] ISSN:2038-8306
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:At present, data on the endocannabinoid system expression and distribution in the pancreatic gland appear scarce and controversial as descriptions are limited to humans and laboratory animals. Since the bovine pancreas is very similar to the human in endocrine portion development and control, studies on the fetal gland could prove to be very interesting, as an abnormal maternal condition during late pregnancy may be a predisposing trigger for adult metabolic disorders. The present investigation studied cannabinoid receptor type 2 presence and distribution in the bovine fetal pancreas towards the end of gestation. Histological analyses revealed numerous endocrinal cell clusters or islets which were distributed among exocrine adenomeri in connectival tissue. Immunohistochemistry showed that endocrine-islets contained some CB2-positive cells with a very peculiar localization that is a few primarily localized at the edges of islets and some of them also scattered in the center of the cluster. Characteristically, also the epithelium of the excretory ducts and the smooth muscle layers of the smaller arteries, in the interlobular glandular septa, tested positive for the CB2 endocannabinoid receptor. Conse - quently, the endocannabinoid system, via the cannabinoid receptor type 2, was hypothesized to play a major role in controlling pancreas function from normal fetal development to correct metabolic functioning in adulthood.
[Mh] Termos MeSH primário: Feto/embriologia
Regulação da Expressão Gênica no Desenvolvimento/fisiologia
Ilhotas Pancreáticas/embriologia
Pâncreas Exócrino/embriologia
Receptor CB2 de Canabinoide/biossíntese
[Mh] Termos MeSH secundário: Animais
Bovinos
Feminino
Feto/citologia
Seres Humanos
Ilhotas Pancreáticas/citologia
Pâncreas Exócrino/citologia
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptor, Cannabinoid, CB2)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE
[do] DOI:10.4081/ejh.2017.2761


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[PMID]:28258566
[Au] Autor:Delporte C
[Ad] Endereço:Laboratory of Pathophysiological and Nutritional Biochemistry, Faculty of Medicine, Université Libre de Bruxelles, Brussels, Belgium. cdelport@ulb.ac.be.
[Ti] Título:Aquaporins and Gland Secretion.
[So] Source:Adv Exp Med Biol;969:63-79, 2017.
[Is] ISSN:0065-2598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aquaporins (AQPs ) are expressed in most exocrine and endocrine secretory glands. Consequently, summarizing the expression and functions of AQPs in secretory glands represents a daunting task considering the important number of glands present in the body, as well as the number of mammalian AQPs - thirteen. The roles played by AQPs in secretory processes have been investigated in many secretory glands. However, despite considerable research, additional studies are clearly needed to pursue our understanding of the role played by AQPs in secretory processes. This book chapter will focus on summarizing the current knowledge on AQPs expression and function in the gastrointestinal tract , including salivary glands, gastric glands, Duodenal Brunner's gland, liver and gallbladder, intestinal goblets cells, exocrine and endocrine pancreas, as well as few other secretory glands including airway submucosal glands, lacrimal glands, mammary glands and eccrine sweat glands.
[Mh] Termos MeSH primário: Aquaporinas/metabolismo
Células Eucarióticas/metabolismo
Mucosa Gástrica/secreção
Glândulas Mamárias Humanas/secreção
Glândulas Salivares/secreção
[Mh] Termos MeSH secundário: Animais
Aquaporinas/química
Aquaporinas/genética
Transporte Biológico
Glândulas Duodenais/metabolismo
Glândulas Duodenais/secreção
Células Eucarióticas/citologia
Mucosa Gástrica/metabolismo
Expressão Gênica
Seres Humanos
Ilhotas Pancreáticas/metabolismo
Ilhotas Pancreáticas/secreção
Aparelho Lacrimal/metabolismo
Aparelho Lacrimal/secreção
Glândulas Mamárias Humanas/metabolismo
Pâncreas Exócrino/metabolismo
Pâncreas Exócrino/secreção
Subunidades Proteicas/química
Subunidades Proteicas/genética
Subunidades Proteicas/metabolismo
Glândulas Salivares/metabolismo
Glândulas Sudoríparas/metabolismo
Glândulas Sudoríparas/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Aquaporins); 0 (Protein Subunits)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE
[do] DOI:10.1007/978-94-024-1057-0_4


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[PMID]:28242761
[Au] Autor:Liang T; Dolai S; Xie L; Winter E; Orabi AI; Karimian N; Cosen-Binker LI; Huang YC; Thorn P; Cattral MS; Gaisano HY
[Ad] Endereço:From the Department of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
[Ti] Título: human pancreatic slice preparations offer a valuable model for studying pancreatic exocrine biology.
[So] Source:J Biol Chem;292(14):5957-5969, 2017 Apr 07.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A genuine understanding of human exocrine pancreas biology and pathobiology has been hampered by a lack of suitable preparations and reliance on rodent models employing dispersed acini preparations. We have developed an organotypic slice preparation of the normal portions of human pancreas obtained from cancer resections. The preparation was assessed for physiologic and pathologic responses to the cholinergic agonist carbachol (Cch) and cholecystokinin (CCK-8), including 1) amylase secretion, 2) exocytosis, 3) intracellular Ca responses, 4) cytoplasmic autophagic vacuole formation, and 5) protease activation. Cch and CCK-8 both dose-dependently stimulated secretory responses from human pancreas slices similar to those previously observed in dispersed rodent acini. Confocal microscopy imaging showed that these responses were accounted for by efficient apical exocytosis at physiologic doses of both agonists and by apical blockade and redirection of exocytosis to the basolateral plasma membrane at supramaximal doses. The secretory responses and exocytotic events evoked by CCK-8 were mediated by CCK-A and not CCK-B receptors. Physiologic agonist doses evoked oscillatory Ca increases across the acini. Supraphysiologic doses induced formation of cytoplasmic autophagic vacuoles and activation of proteases (trypsin, chymotrypsin). Maximal atropine pretreatment that completely blocked all the Cch-evoked responses did not affect any of the CCK-8-evoked responses, indicating that rather than acting on the nerves within the pancreas slice, CCK cellular actions directly affected human acinar cells. Human pancreas slices represent excellent preparations to examine pancreatic cell biology and pathobiology and could help screen for potential treatments for human pancreatitis.
[Mh] Termos MeSH primário: Exocitose
Técnicas de Preparação Histocitológica/métodos
Modelos Biológicos
Pâncreas Exócrino/metabolismo
Pancreatite/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Feminino
Seres Humanos
Masculino
Meia-Idade
Pâncreas Exócrino/patologia
Pancreatite/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.777433



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