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[PMID]:29184405
[Au] Autor:Hureaux J; Lacoeuille F; Lagarce F; Rousselet MC; Contini A; Saulnier P; Benoit JP; Urban T
[Ad] Endereço:Unité Micro et Nanomédecines Biomimétiques (MINT), Université d'Angers, INSERM 1066, CNRS 6021, Université Bretagne Loire.
[Ti] Título:Absence of lung fibrosis after a single pulmonary delivery of lipid nanocapsules in rats.
[So] Source:Int J Nanomedicine;12:8159-8170, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Lipid nanocapsules (LNCs) are potential drug carriers for pulmonary delivery since they can be nebulized without any structural or functional changes, and the aerosols produced are highly compatible with pulmonary drug delivery in human beings. The alveolar surface tension, in vitro cytotoxicity, biodistribution and pulmonary toxicity in rats of a single endotracheal spray of LNCs or paclitaxel-loaded LNCs were studied. In vitro cytotoxicity of LNCs after a spray remained unchanged. Biodistribution study showed a homogeneous repartition in the lungs in rats with an improvement in lung retention of the radiolabeled tracer loaded in LNCs compared to the absence of LNCs with a lung half-time of 8.8±0.7 hours. Bronchoalveolar fluid analysis revealed transient 7-day alveolar inflammation, reaching a maximum between days 2 and 4, characterized by a peak of granulocytes at day 1 followed by a peak of lymphocytes at day 3. Alveolar protein levels were increased at days 3 and 7. Acute inflammation was increased with paclitaxel-loaded LNCs in comparison with blank LNCs but dropped out at day 7. No histological pulmonary lesion was observed at day 60. LNCs lowered surface tension to a greater degree than Curosurf in a physicochemical model of the pulmonary alveolus. A single pulmonary delivery of LNCs induces a short-term alveolar inflammation with no residual lesions in rats at day 60. These data permit to start the study of LNCs in surfactant replacement therapy.
[Mh] Termos MeSH primário: Portadores de Fármacos/efeitos adversos
Portadores de Fármacos/farmacocinética
Pulmão/efeitos dos fármacos
Pulmão/patologia
Nanocápsulas/efeitos adversos
[Mh] Termos MeSH secundário: Aerossóis/administração & dosagem
Aerossóis/efeitos adversos
Aerossóis/química
Animais
Produtos Biológicos
Linhagem Celular
Portadores de Fármacos/administração & dosagem
Sistemas de Liberação de Medicamentos/efeitos adversos
Sistemas de Liberação de Medicamentos/métodos
Feminino
Fibrose
Seres Humanos
Lipídeos/química
Nanocápsulas/administração & dosagem
Nanocápsulas/química
Paclitaxel/química
Paclitaxel/farmacocinética
Fosfolipídeos
Alvéolos Pulmonares/efeitos dos fármacos
Alvéolos Pulmonares/fisiopatologia
Ratos Sprague-Dawley
Tensão Superficial/efeitos dos fármacos
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Biological Products); 0 (Drug Carriers); 0 (Lipids); 0 (Nanocapsules); 0 (Phospholipids); KE3U2023NP (poractant alfa); P88XT4IS4D (Paclitaxel)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S146740


  2 / 23324 MEDLINE  
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[PMID]:29267317
[Au] Autor:Hassan T; Thiberville L; Hermant C; Lachkar S; Piton N; Guisier F; Salaun M
[Ad] Endereço:Department of Respiratory Care, Thoracic Oncology, and Respiratory Intensive Care & CIC-CRB U1404, Rouen University Hospital, Rouen, France.
[Ti] Título:Assessing the feasibility of confocal laser endomicroscopy in solitary pulmonary nodules for different part of the lungs, using either 0.6 or 1.4 mm probes.
[So] Source:PLoS One;12(12):e0189846, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Malignant solitary pulmonary nodules (SPN) have become more prevalent, with upper lobes predilection. Probe-based confocal laser endomicroscopy (pCLE) provides in-vivo imaging of SPN. However, the stiffness of the 1mm confocal probe (AlveoFlex) causes difficult accessibility to the upper lobes. A thinner 600µm probe designed for bile duct exploration (CholangioFlex) has the potential to reach the upper lobes. OBJECTIVES: To examine the accessibility of malignant SPNs in all segments of the lungs using either the 0.6mm or 1.4 mm probe and to assess the quality and inter observer interpretation of SPN confocal imaging obtained from either miniprobes. METHODS: Radial(r)-EBUS was used to locate and sample the SPN. In-vivo pCLE analysis of the SPN was performed using either CholangioFlex (apical and posterior segments of the upper lobes) or AlveoFlex (other segments) introduced into the guide sheath before sampling. pCLE features were compared between the two probes. RESULTS: Fourty-eight patients with malignant SPN were included (NCT01931579). The diagnostic accuracy for lung cancer using r-EBUS coupled with pCLE imaging was 79.2%. All the SPNs were successfully explored with either one of the probes (19 and 29 subjects for CholangioFlex and AlveoFlex, respectively). A specific solid pattern in the SPN was found in 30 pCLE explorations. Comparison between the two probes found no differences in the axial fibers thickness, cell size and specific solid pattern in the nodules. Extra-alveolar microvessel size appeared larger using CholangioFlex suggesting less compression effect. The kappa test for interobserver agreement for the identification of solid pattern was 0.74 (p = 0.001). CONCLUSION: This study demonstrates that pCLE imaging of SPNs is achievable in all segments of both lungs using either the 0.6mm or 1.4mm miniprobe.
[Mh] Termos MeSH primário: Microscopia Confocal/métodos
Nódulo Pulmonar Solitário/patologia
[Mh] Termos MeSH secundário: Idoso
Estudos de Viabilidade
Feminino
Seres Humanos
Masculino
Microscopia Confocal/instrumentação
Meia-Idade
Variações Dependentes do Observador
Projetos Piloto
Alvéolos Pulmonares/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189846


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[PMID]:29324866
[Au] Autor:More S; Yang X; Zhu Z; Bamunuarachchi G; Guo Y; Huang C; Bailey K; Metcalf JP; Liu L
[Ad] Endereço:The Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, Oklahoma, United States of America.
[Ti] Título:Regulation of influenza virus replication by Wnt/ß-catenin signaling.
[So] Source:PLoS One;13(1):e0191010, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wnt/ß-catenin signaling is an essential pathway in cell cycle control. Dysregulation of the Wnt/ß-catenin signaling pathway during viral infection has been reported. In this study, we examined the effect of modulating Wnt/ß-catenin signaling during influenza virus infection. The activation of the Wnt/ß-catenin pathway by Wnt3a increased influenza virus mRNA and virus production in in vitro in mouse lung epithelial E10 cells and mRNA expresson of influenza virus genes in vivo in the lungs of mice infected with influenza virus A/Puerto Rico/8/34. However, the inhibition of Wnt/ß-catenin signaling by iCRT14 reduced virus titer and viral gene expression in human lung epithelial A549 cells and viral replication in primary mouse alveolar epithelial cells infected with different influenza virus strains. Knockdown of ß-catenin also reduced viral protein expression and virus production. iCRT14 acts at the early stage of virus replication. Treatment with iCRT14 inhibited the expression of the viral genes (vRNA, cRNA and mRNA) evaluated in this study. The intraperitoneal administration of iCRT14 reduced viral load, improved clinical signs, and partially protected mice from influenza virus infection.
[Mh] Termos MeSH primário: Vírus da Influenza A/fisiologia
Replicação Viral
Via de Sinalização Wnt
[Mh] Termos MeSH secundário: Células A549
Animais
Cães
Seres Humanos
Vírus da Influenza A/genética
Células Madin Darby de Rim Canino
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Alvéolos Pulmonares/virologia
RNA Viral/biossíntese
Proteína Wnt3A/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Wnt3A Protein)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191010


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[PMID]:29382022
[Au] Autor:Wang HX; Miao HH; Gao X; Wei W; Ding GN; Zhang Y; Tian M
[Ad] Endereço:Department of Anesthesiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
[Ti] Título:Optimum end-tidal concentration of sevoflurane to facilitate supraglottic airway device insertion with propofol at induction allowing spontaneous respiration in obese patients: A prospective observational study.
[So] Source:Medicine (Baltimore);96(47):e8902, 2017 Nov.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Obese patients are more likely to encounter with difficult airway management, and supraglottic airway device has been adopted to facilitate tracheal intubation. The optimum anesthetic concentration for obese patients to insert a supraglottic airway device with spontaneous respiration has not been investigated. This study was designed to determine the end-tidal concentration of sevoflurane that would provide acceptable condition for supraglottic airway device insertion with propofol at induction in obese patients without using neuromuscular blockade.Thirty elective obese patients [body mass index (BMI) 30-50 kg/m] scheduled for bariatric surgery were enrolled in this study. Sevoflurane was inhaled at a concentration of 5% after infusion of 1 mg/kg propofol (within 1 minute) according to lean body weight. The target concentration of sevoflurane was initiated at 2.5% with 0.5% as a step size using a modified Dixon up-and-down method. Five minutes after target concentration achieved, the insertion of supraglottic airway device was attempted.The minimum alveolar concentration of sevoflurane for successful insertion of supraglottic airway device calculated using up-and-down method were 2.25 (0.53) % for obese patients. The values of the effective concentration of sevoflurane for successful supraglottic airway device insertion in 50% (EC50) and 95% (EC95) of the obese patients obtained by probit regression analysis were 2.09% (95% confidence interval 1.48-2.68) and 3.31% (95% confidence interval 2.70-8.12), respectively.We conclude that sevoflurane at a minimum alveolar concentration of 2.25% can provide optimal conditions for insertion of supraglottic airway device with spontaneous respiration in obese patients with 1 mg/kg propofol at induction.
[Mh] Termos MeSH primário: Manuseio das Vias Aéreas/métodos
Anestésicos Inalatórios/administração & dosagem
Intubação Intratraqueal/métodos
Éteres Metílicos/administração & dosagem
Obesidade/cirurgia
[Mh] Termos MeSH secundário: Adulto
Anestésicos Inalatórios/análise
Anestésicos Intravenosos/administração & dosagem
Cirurgia Bariátrica
Relação Dose-Resposta a Droga
Feminino
Seres Humanos
Masculino
Éteres Metílicos/análise
Propofol/administração & dosagem
Estudos Prospectivos
Alvéolos Pulmonares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Anesthetics, Inhalation); 0 (Anesthetics, Intravenous); 0 (Methyl Ethers); 38LVP0K73A (sevoflurane); YI7VU623SF (Propofol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008902


  5 / 23324 MEDLINE  
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[PMID]:29287116
[Au] Autor:Dravet-Gounot P; Morin C; Jacques S; Dumont F; Ely-Marius F; Vaiman D; Jarreau PH; Méhats C; Zana-Taïeb E
[Ad] Endereço:Inserm, U1016, Institut Cochin, Paris, France.
[Ti] Título:Lung microRNA deregulation associated with impaired alveolarization in rats after intrauterine growth restriction.
[So] Source:PLoS One;12(12):e0190445, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intrauterine growth restriction (IUGR) was recently described as an independent risk factor of bronchopulmonary dysplasia, the main respiratory sequelae of preterm birth. We previously showed impaired alveolarization in rat pups born with IUGR induced by a low-protein diet (LPD) during gestation. We conducted a genome-wide analysis of gene expression and found the involvement of several pathways such as cell adhesion. Here, we describe our unbiased microRNA (miRNA) profiling by microarray assay and validation by qPCR at postnatal days 10 and 21 (P10 and P21) in lungs of rat pups with LPD-induced lung-alveolarization disorder after IUGR. We identified 13 miRNAs with more than two-fold differential expression between control lungs and LPD-induced IUGR lungs. Validated and predicted target genes of these miRNAs were related to "tissue repair" at P10 and "cellular communication regulation" at P21. We predicted the deregulation of several genes associated with these pathways. Especially, E2F3, a transcription factor involved in cell cycle control, was expressed in developing alveoli, and its mRNA and protein levels were significantly increased at P21 after IUGR. Hence, IUGR affects the expression of selected miRNAs during lung alveolarization. These results provide a basis for deciphering the mechanistic contributions of IUGR to impaired alveolarization.
[Mh] Termos MeSH primário: Retardo do Crescimento Fetal
MicroRNAs/genética
Alvéolos Pulmonares/patologia
[Mh] Termos MeSH secundário: Animais
Feminino
Perfilação da Expressão Gênica
Masculino
Alvéolos Pulmonares/metabolismo
Ratos
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190445


  6 / 23324 MEDLINE  
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[PMID]:28457424
[Au] Autor:Pinto H; Leal R; Rodrigues L; Santos L; Romãozinho C; Macário F; Alves R; Pratas J; Sousa V; Marinho C; Prado E Castro L; Costa F; Campos M; Mota A; Figueiredo A
[Ad] Endereço:Nephrology Department, Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal. Electronic address: helenasofiapinto@gmail.com.
[Ti] Título:What Can We Do When All Collapses? Fatal Outcome of Collapsing Glomerulopathy and Systemic Lupus Erythematosus With Diffuse Alveolar Hemorrhage: Case Report.
[So] Source:Transplant Proc;49(4):913-915, 2017 May.
[Is] ISSN:1873-2623
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Collapsing glomerulopathy (CG) is a rare form of glomerular injury. Although commonly associated with human immunodeficiency virus (HIV) infection, it can occur in association with systemic lupus erythematosus (SLE). CASE REPORT: We present the case of a 50-year-old man, with chronic kidney disease secondary to focal and segmental glomerulosclerosis, who received a cadaveric kidney transplant in 2007. There were no relevant intercurrences until May 2015, when he presented with nephrotic range proteinuria (± 4 g/d). A graft biopsy was performed and it did not show any significant pathological changes. In September, he developed a full nephrotic syndrome (proteinuria 19 g/d) and a graft biopsy was repeated. CG features were evident with a rich immunofluorescence. Antinuclear antibodies (ANA) and anti-double-stranded DNA (anti-dsDNA) antibodies were positive; the remaining immunologic study was normal. Viral markers for HIV, hepatitis C virus (HCV), and hepatitis B virus (HBV) were negative. The patient was treated with corticosteroid pulses and plasmapheresis (seven treatments). A rapid deterioration of kidney function was seen and he became dialysis dependent. He was discharged with a low-dose immunosuppressive treatment. In October, he was hospitalized with diffuse alveolar hemorrhage (DAH). The auto-immune study was repeated, revealing complement consumption and positive titers of ANA and Anti-dsDNA antibodies. Anti-neutrophil cytoplasmic antibodies (ANCAs) and antiglomerular basement membrane antibody (anti-GBM) were negative. Treatment with intravenous corticosteroids, plasmapheresis, and human immunoglobulin was ineffective and the outcome was fatal. CONCLUSION: This case report highlights the possible association of CG and SLE. To our knowledge, it is the first case of SLE presenting with CG and DAH, with the singularity of occurring in a kidney transplant recipient receiving immunosuppression.
[Mh] Termos MeSH primário: Hemorragia/imunologia
Lúpus Eritematoso Sistêmico/complicações
Nefrite Lúpica/imunologia
Complicações Pós-Operatórias/imunologia
Alvéolos Pulmonares
[Mh] Termos MeSH secundário: Anticorpos Antinucleares/sangue
Biópsia
Evolução Fatal
Glomerulosclerose Segmentar e Focal/complicações
Glomerulosclerose Segmentar e Focal/imunologia
Glomerulosclerose Segmentar e Focal/cirurgia
Hemorragia/patologia
Seres Humanos
Imunossupressores/uso terapêutico
Glomérulos Renais/imunologia
Transplante de Rim/efeitos adversos
Lúpus Eritematoso Sistêmico/imunologia
Nefrite Lúpica/patologia
Masculino
Meia-Idade
Síndrome Nefrótica/sangue
Síndrome Nefrótica/etiologia
Síndrome Nefrótica/imunologia
Síndrome Nefrótica/patologia
Complicações Pós-Operatórias/patologia
Proteinúria/imunologia
Proteinúria/patologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Antinuclear); 0 (Immunosuppressive Agents)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  7 / 23324 MEDLINE  
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[PMID]:29206862
[Au] Autor:Bao A; Che KF; Bozinovski S; Ji J; Gregory JA; Kumlien Georén S; Adner M; Cardell LO; Lindén A
[Ad] Endereço:Unit for Lung and Airway Research, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
[Ti] Título:Recombinant human IL-26 facilitates the innate immune response to endotoxin in the bronchoalveolar space of mice in vivo.
[So] Source:PLoS One;12(12):e0188909, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Interleukin (IL)-26 is released in response to bacterial endotoxin (LPS) in the bronchoalveolar space of humans in vivo and it may potentiate neutrophil chemotaxis by enhanced IL-26 receptor stimulation. However, the effects of extracellular IL-26 protein on the innate immune response in the lungs in vivo remain unknown. Here, we characterized these effects of IL-26 on a wide range of aspects of the innate immune response to LPS in different compartments of the lungs in vivo over time. We administrated recombinant human (rh) IL-26 protein in the bronchoalveolar space using intranasal instillation in a mouse in vivo model, with and without prior instillation of LPS. We verified gene expression of the IL-26 receptor complex in mouse lungs and observed that, after instillation of LPS, rhIL-26 increases the phosphorylation of STAT3, a signaling molecule of the IL-26 receptor complex. We also observed that rhIL-26 exerted additional stimulatory and inhibitory actions that are compartment- and time-dependent, resulting in alterations of cytokines, proteinases, tissue inflammation and the accumulation of innate effector cells. Without the prior instillation of LPS, rhIL-26 exerted time-dependent effects on total gelatinase activity but few other effects. Most important, after instillation of LPS, rhIL-26 cleared inflammatory cells from local tissue and increased the accumulation of innate effector cells in the bronchoalveolar space. Tentatively, rhIL-26 may facilitate the innate immune response towards the bronchoalveolar space in vivo and represents a potential target for therapy in lung disorders involving the innate immune response.
[Mh] Termos MeSH primário: Brônquios/efeitos dos fármacos
Imunidade Inata/efeitos dos fármacos
Interleucinas/farmacologia
Lipopolissacarídeos/farmacologia
Alvéolos Pulmonares/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Brônquios/imunologia
Líquido da Lavagem Broncoalveolar
Citocinas/biossíntese
Seres Humanos
Camundongos
Camundongos Endogâmicos BALB C
Peroxidase/metabolismo
Fosforilação
Alvéolos Pulmonares/imunologia
RNA Mensageiro/genética
Proteínas Recombinantes/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (IL26 protein, human); 0 (Interleukins); 0 (Lipopolysaccharides); 0 (RNA, Messenger); 0 (Recombinant Proteins); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188909


  8 / 23324 MEDLINE  
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[PMID]:29176899
[Au] Autor:Smith LJ; Macleod KA; Collier GJ; Horn FC; Sheridan H; Aldag I; Taylor CJ; Cunningham S; Wild JM; Horsley A
[Ad] Endereço:POLARIS, Academic Radiology, University of Sheffield, Sheffield, United Kingdom.
[Ti] Título:Supine posture changes lung volumes and increases ventilation heterogeneity in cystic fibrosis.
[So] Source:PLoS One;12(11):e0188275, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Lung Clearance Index (LCI) is recognised as an early marker of cystic fibrosis (CF) lung disease. The effect of posture on LCI however is important when considering longitudinal measurements from infancy and when comparing LCI to imaging studies. METHODS: 35 children with CF and 28 healthy controls (HC) were assessed. Multiple breath washout (MBW) was performed both sitting and supine in triplicate and analysed for LCI, Scond, Sacin, and lung volumes. These values were also corrected for the Fowler dead-space to create 'alveolar' indices. RESULTS: From sitting to supine there was a significant increase in LCI and a significant decrease in FRC for both CF and HC (p<0.01). LCI, when adjusted to estimate 'alveolar' LCI (LCIalv), increased the magnitude of change with posture for both LCIalv and FRCalv in both groups, with a greater effect of change in lung volume in HC compared with children with CF. The % change in LCIalv for all subjects correlated significantly with lung volume % changes, most notably tidal volume/functional residual capacity (Vtalv/FRCalv (r = 0.54,p<0.001)). CONCLUSION: There is a significant increase in LCI from sitting to supine, which we believe to be in part due to changes in lung volume and also increasing ventilation heterogeneity related to posture. This may have implications in longitudinal measurements from infancy to older childhood and for studies comparing supine imaging methods to LCI.
[Mh] Termos MeSH primário: Fibrose Cística/patologia
Fibrose Cística/fisiopatologia
Pulmão/patologia
Pulmão/fisiopatologia
Ventilação Pulmonar
Decúbito Dorsal
[Mh] Termos MeSH secundário: Estudos de Casos e Controles
Criança
Feminino
Capacidade Residual Funcional
Seres Humanos
Masculino
Tamanho do Órgão
Alvéolos Pulmonares/patologia
Alvéolos Pulmonares/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188275


  9 / 23324 MEDLINE  
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[PMID]:28967890
[Au] Autor:Yamamoto Y; Gotoh S; Korogi Y; Seki M; Konishi S; Ikeo S; Sone N; Nagasaki T; Matsumoto H; Muro S; Ito I; Hirai T; Kohno T; Suzuki Y; Mishima M
[Ad] Endereço:Department of Respiratory Medicine, Kyoto University, Kyoto, Japan.
[Ti] Título:Long-term expansion of alveolar stem cells derived from human iPS cells in organoids.
[So] Source:Nat Methods;14(11):1097-1106, 2017 Nov.
[Is] ISSN:1548-7105
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The stable expansion of tissue-specific stem cells in vitro has contributed to research on several organs. Alveolar epithelial type II (AT2) cells function as tissue stem cells in the lung, but robust models for studying human AT2 cells are lacking. Here we report a method for the efficient generation and long-term expansion of alveolar organoids (AOs) harboring SFTPC alveolar stem cells derived from human induced pluripotent stem cells (hiPSCs). hiPSC-derived SFTPC cells self-renewed, with transcriptomes and morphology consistent with those of AT2 cells, and were able to differentiate into alveolar epithelial type I (AT1)-like cells. Single-cell RNA-seq of SFTPC cells and their progenitors demonstrated that their differentiation process and cellular heterogeneity resembled those of developing AT2 cells in vivo. AOs were applicable to drug toxicology studies recapitulating AT2-cell-specific phenotypes. Our methods can help scientists overcome the limitations of current approaches to the modeling of human alveoli and should be useful for disease modeling and regenerative medicine.
[Mh] Termos MeSH primário: Células-Tronco Pluripotentes Induzidas/química
Organoides/metabolismo
Alvéolos Pulmonares/citologia
[Mh] Termos MeSH secundário: Linhagem Celular
Seres Humanos
Células-Tronco Pluripotentes Induzidas/efeitos dos fármacos
Alvéolos Pulmonares/efeitos dos fármacos
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de RNA
Análise de Célula Única
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE
[do] DOI:10.1038/nmeth.4448


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Amato, Marcelo Britto Passos
Texto completo
[PMID]:28961282
[Au] Autor:Cordioli RL; Costa ELV; Azevedo LCP; Gomes S; Amato MBP; Park M
[Ad] Endereço:Research and Education Institute, Hospital Sírio-Libanês, São Paulo, Brazil.
[Ti] Título:Physiologic effects of alveolar recruitment and inspiratory pauses during moderately-high-frequency ventilation delivered by a conventional ventilator in a severe lung injury model.
[So] Source:PLoS One;12(9):e0185769, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: To investigate whether performing alveolar recruitment or adding inspiratory pauses could promote physiologic benefits (VT) during moderately-high-frequency positive pressure ventilation (MHFPPV) delivered by a conventional ventilator in a porcine model of severe acute respiratory distress syndrome (ARDS). METHODS: Prospective experimental laboratory study with eight pigs. Induction of acute lung injury with sequential pulmonary lavages and injurious ventilation was initially performed. Then, animals were ventilated on a conventional mechanical ventilator with a respiratory rate (RR) = 60 breaths/minute and PEEP titrated according to ARDS Network table. The first two steps consisted of a randomized order of inspiratory pauses of 10 and 30% of inspiratory time. In final step, we removed the inspiratory pause and titrated PEEP, after lung recruitment, with the aid of electrical impedance tomography. At each step, PaCO2 was allowed to stabilize between 57-63 mmHg for 30 minutes. RESULTS: The step with RR of 60 after lung recruitment had the highest PEEP when compared with all other steps (17 [16,19] vs 14 [10, 17]cmH2O), but had lower driving pressures (13 [13,11] vs 16 [14, 17]cmH2O), higher P/F ratios (212 [191,243] vs 141 [105, 184] mmHg), lower shunt (23 [20, 23] vs 32 [27, 49]%), lower dead space ventilation (10 [0, 15] vs 30 [20, 37]%), and a more homogeneous alveolar ventilation distribution. There were no detrimental effects in terms of lung mechanics, hemodynamics, or gas exchange. Neither the addition of inspiratory pauses or the alveolar recruitment maneuver followed by decremental PEEP titration resulted in further reductions in VT. CONCLUSIONS: During MHFPPV set with RR of 60 bpm delivered by a conventional ventilator in severe ARDS swine model, neither the inspiratory pauses or PEEP titration after recruitment maneuver allowed reduction of VT significantly, however the last strategy decreased driving pressures and improved both shunt and dead space.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Ventilação de Alta Frequência
Lesão Pulmonar/fisiopatologia
Alvéolos Pulmonares/fisiologia
[Mh] Termos MeSH secundário: Animais
Masculino
Estudos Prospectivos
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170930
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185769



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