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  1 / 16101 MEDLINE  
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[PMID]:28466357
[Au] Autor:Kavuzlu A; Tatar EÇ; Karagöz T; Pinarli FA; Tatar I; Bayir Ö; Korkmaz MH
[Ad] Endereço:Department of Otorhinolaryngology and Head and Neck Surgery, Diskapi Yildirim Beyazit Research and Training Hospital, Ministiry of Health, Ankara, Turkey. akavuzlu@hotmail.com.
[Ti] Título:The effects of the stem cell on ciliary regeneration of injured rabbit sinonasal epithelium.
[So] Source:Eur Arch Otorhinolaryngol;274(8):3057-3064, 2017 Aug.
[Is] ISSN:1434-4726
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Defects in mucosal healing after sinonasal surgery cause infection, scar formation causing obstruction, relapse of the disease within a shorter period and revision surgery. The present study aimed to create a functional ciliated epithelium using a stem cell and stem cell sheet of adipose tissue origin and to show such regeneration ultra-structurally on experimentally injured rabbit nasal epithelium. This was an experimental animal study and basic research. A total of 18 white New Zealand rabbits were divided into three groups. The medial wall of the maxillary sinus of the subjects was peeled off bilaterally. No additional procedure was applied to the subjects in Group 1. In Group 2, adipose tissue-derived mesenchymal stem cell was implanted on the wound edges of the subjects. In Group 3, a stem cell sheet of three layers was laid onto the defect area. All subjects were killed after 3 weeks. The presence of the stem cell stained with bromo-deoxyuridine was assessed with a light microscope, whereas cilia density, ciliated orientation and cilia structure were evaluated with a scanning electron microscope. Ciliary densities in Group 2 and Group 3 were statistically superior compared to the control group (p < 0.001, p = 0.007). Cilia morphology in Group 2 and Group 3 was also better than the control group (p < 0.01, p = 0.048). Ciliary orientation in Group 2 was scored highest (p < 0.01). The ratio of BrDu-stained cells was observed to be 27% in Group 3 and 8% in Group 2. Sub-epithelial recovery was observed to be better in Group 3. Adipose tissue-derived mesenchymal stem cell increased the healing of the injured maxillary sinus mucosa of the rabbits in terms of cilia presence, density and morphology regardless of the implementation technique. Level of evidence NA.
[Mh] Termos MeSH primário: Cílios/fisiologia
Transplante de Células-Tronco Mesenquimais/métodos
Células Mesenquimais Estromais/fisiologia
Mucosa Nasal
Cicatrização/fisiologia
[Mh] Termos MeSH secundário: Tecido Adiposo/citologia
Animais
Masculino
Seio Maxilar/patologia
Seio Maxilar/fisiopatologia
Seio Maxilar/cirurgia
Modelos Animais
Mucosa Nasal/lesões
Mucosa Nasal/patologia
Mucosa Nasal/fisiopatologia
Procedimentos Cirúrgicos Nasais
Coelhos
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s00405-017-4595-7


  2 / 16101 MEDLINE  
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[PMID]:28452704
[Au] Autor:Yan CH; Hahn S; McMahon D; Bonislawski D; Kennedy DW; Adappa ND; Palmer JN; Jiang P; Lee RJ; Cohen NA
[Ad] Endereço:Department of Otorhinolaryngology-Head and Neck Surgery, Division of Rhinology, University of Pennsylvania, Perelman School of Medicine, Philadelphia, Pennsylvania, USA.
[Ti] Título:Nitric oxide production is stimulated by bitter taste receptors ubiquitously expressed in the sinonasal cavity.
[So] Source:Am J Rhinol Allergy;31(2):85-92, 2017 Mar 01.
[Is] ISSN:1945-8932
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bitter taste receptors (T2R) have recently been demonstrated to contribute to sinonasal innate immunity. One T2R, T2R38, regulates mucosal defense against gram-negative organisms through nitric oxide (NO) production, which enhances mucociliary clearance and directly kills bacteria. To determine whether additional T2Rs contribute to this innate defense, we evaluated two other sinonasal T2Rs (T2R4 and T2R16) for regulation of NO production and expression within the human sinonasal cavity. METHODS: Primary human sinonasal cultures were stimulated with ligands specific to T2R4 and T2R16, colchicine and D-salicin, respectively. Cellular NO production was measured by intracellular 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate fluorescence. For T2R expression mapping, sinonasal tissue was obtained from patients who underwent sinus surgery of the middle turbinate, maxillary sinus, ethmoid sinus, or sphenoid sinus. The expression of T2R4, T2R16, and T2R38 was evaluated by using immunofluorescence with validated antibodies. RESULTS: Similar to T2R38, T2R4 and T2R16 trigger NO production in a dose-dependent manner by using the canonical taste signaling pathway in response to stimulation with their respective ligands. All three receptors were expressed in the cilia of human epithelial cells of all regions in the sinonasal cavity. CONCLUSION: These three T2Rs signaled through the same NO-mediated antimicrobial pathway and were ubiquitously expressed in the sinonasal epithelium. Additional T2Rs besides T2R38 may play a role in sinonasal immune defense. Mapping of T2R expression demonstrated the potential widespread role of T2Rs in sinonasal defense, whereas the genetics of these T2Rs may contribute to our understanding of specific endotypes of chronic rhinosinusitis and develop into novel therapeutic targets.
[Mh] Termos MeSH primário: Infecções Bacterianas/imunologia
Mucosa Nasal/imunologia
Seios Paranasais/metabolismo
Receptores Acoplados a Proteínas-G/metabolismo
Rinite/imunologia
Sinusite/imunologia
Paladar
[Mh] Termos MeSH secundário: Bacteriólise
Células Cultivadas
Doença Crônica
Seres Humanos
Imunidade Inata
Depuração Mucociliar
Mucosa Nasal/microbiologia
Óxido Nítrico/metabolismo
Cultura Primária de Células
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, G-Protein-Coupled); 0 (taste receptors, type 2); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.2500/ajra.2017.31.4424


  3 / 16101 MEDLINE  
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[PMID]:29429191
[Au] Autor:Zhang YY; Lou HF; Wang CS; Zhang L
[Ad] Endereço:Deparment of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China; Beijing Key Laboratory of Nasal Diseases, Beijing Institude of Otorhinolaryngology, Beijing 100005, China.
[Ti] Título:[Mechanisms underlying glucocorticoid resistance in chronic rhinosinusitis with nasal polyps].
[So] Source:Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi;53(2):154-160, 2018 Feb 07.
[Is] ISSN:1673-0860
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Chronic rhinosinusitis with nasal polyps (CRSwNP) is a chronic inflammatory disease that occurs in the nasal and sinus mucosa, which is a common disease in otorhinolaryngology. At present, CRSwNP can be effectively treated by glucocorticoids (GC). GC binds to GC receptors in the nasal mucosa, affects the expression of inflammatory genes, inhibits the activation and action of eosinophils, T cell-associated inflammatory responses in nasal polyps, as well as tissue remodeling. However, there are some patients fall reponse to GC, so called GC resistance. The study suggests that the possible mechanism of CRSwNP GC resistance is mainly related to GC receptor abnormal, the role of cytokines and transcription factors, such as Th cells and IL-8. In addition, MAPK-related kinases and histone deacetylase in the GC signaling pathway also play important roles in the GC resistance process. This paper reviews the mechanism of GC treatment of CRSwNP, the mechanism of GC resistance and alternative treatment of GC.
[Mh] Termos MeSH primário: Glucocorticoides/uso terapêutico
Erros Inatos do Metabolismo
Pólipos Nasais/tratamento farmacológico
Receptores de Glucocorticoides/deficiência
Rinite/tratamento farmacológico
Sinusite/tratamento farmacológico
[Mh] Termos MeSH secundário: Doença Crônica
Citocinas/metabolismo
Resistência a Medicamentos
Glucocorticoides/metabolismo
Seres Humanos
Mucosa Nasal/metabolismo
Pólipos Nasais/complicações
Rinite/complicações
Transdução de Sinais
Sinusite/complicações
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cytokines); 0 (Glucocorticoids); 0 (Receptors, Glucocorticoid); 0 (Transcription Factors)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.1673-0860.2018.02.017


  4 / 16101 MEDLINE  
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[PMID]:28470140
[Au] Autor:Carson JL; Zhou L; Brighton L; Mills KH; Zhou H; Jaspers I; Hazucha M
[Ad] Endereço:a The Center for Environmental Medicine, Asthma, and Lung Biology , The University of North Carolina at Chapel Hill , Chapel Hill , NC , USA.
[Ti] Título:Temporal structure/function variation in cultured differentiated human nasal epithelium associated with acute single exposure to tobacco smoke or E-cigarette vapor.
[So] Source:Inhal Toxicol;29(3):137-144, 2017 02.
[Is] ISSN:1091-7691
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Mucociliary clearance sustains a baseline functionality and an "on demand" capability to upregulate clearance upon irritant exposure involving mucus hypersecretion and accelerated ciliary beat frequency (CBF) modulated by nitric oxide (NO). This study characterized these elements as well as cellular and exogenous NO concentrations subsequent to a single exposure to tobacco smoke (TS) or e-cigarette vapor (EV) on cultured human airway epithelium. MATERIALS AND METHODS: Air-liquid interface (ALI) airway epithelial cultures per nonsmoking human subjects were subjected to single TS or EV exposures. Measures of ciliary function and secretion were performed and cellular and exogenous NO concentrations under control and experimental conditions were assessed. RESULTS: Both TS and EV exposures resulted similar patterns of decline in CBF within 1 min of the completion of exposure followed by a gradual return often exceeding baseline within 1 h. Post-exposure examination of exposed cultures suggested morphologic differences in secretory function relative to controls. The relative NO concentrations of TS and EV chamber air were sharply different with EV NO being only slightly elevated relative to cellular NO production. DISCUSSION AND CONCLUSIONS: Epithelial remodeling and mucociliary dysfunction have been clearly associated with TS exposure. However, information contrasting epithelial structure/function following a single acute TS or EV exposure is limited. This study demonstrates a similar pattern of epithelial response to acute TS or EV exposure. Inasmuch as NO may contribute to an inflammatory milieu and generation of toxic metabolites, it is plausible that recurrent exposures over time may be contributory to chronic pathologies.
[Mh] Termos MeSH primário: Sistemas Eletrônicos de Liberação de Nicotina
Mucosa Nasal/efeitos dos fármacos
Fumaça/efeitos adversos
Tabaco
[Mh] Termos MeSH secundário: Diferenciação Celular
Células Cultivadas
Cílios/efeitos dos fármacos
Cílios/fisiologia
Seres Humanos
Microscopia Eletrônica de Varredura
Depuração Mucociliar
Mucosa Nasal/citologia
Mucosa Nasal/metabolismo
Mucosa Nasal/ultraestrutura
Óxido Nítrico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Smoke); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1080/08958378.2017.1318985


  5 / 16101 MEDLINE  
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[PMID]:29370185
[Au] Autor:Kim H; Kimoto T; Sakai S; Takahashi E; Kido H
[Ad] Endereço:Division of Enzyme Chemistry, Institute for Enzyme Research, Tokushima University, Tokushima, Japan.
[Ti] Título:Adjuvanting influenza hemagglutinin vaccine with a human pulmonary surfactant-mimicking synthetic compound SF-10 induces local and systemic cell-mediated immunity in mice.
[So] Source:PLoS One;13(1):e0191133, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We reported previously that intranasal instillation of a synthetic human pulmonary surfactant with a carboxy vinyl polymer as a viscosity improver, named SF-10, shows potent adjuvanticity for humoral immunity in mice and cynomolgus monkeys. SF-10 effectively induces influenza hemagglutinin vaccine (HAv)-specific IgA in nasal and lung washes and IgG in sera with their neutralizing activities. Since CD8+ T cell-mediated protection is an important requirement for adaptive immunity, we investigated in this study the effects of SF-10 with antigen on local and systemic cell-mediated immunity. Nasal instillation of ovalbumin, a model antigen, combined with SF-10 efficiently delivered antigen to mucosal dendritic and epithelial cells and promoted cross-presentation in antigen presenting cells, yielding a high percentage of ovalbumin-specific cytotoxic T lymphocytes in the nasal mucosa, compared with ovalbumin alone. Nasal immunization of HAv-SF-10 also induced HAv-specific cytotoxic T lymphocytes and upregulated granzyme B expression in splenic CD8+ T cells with their high cytotoxicity against target cells pulsed with HA peptide. Furthermore, nasal vaccination of HAv-SF-10 significantly induced higher cytotoxic T lymphocytes-mediated cytotoxicity in the lungs and cervical lymph nodes in the early phase of influenza virus infection compared with HAv alone. Protective immunity induced by HAv-SF-10 against lethal influenza virus infection was partially and predominantly suppressed after depletion of CD8+ and CD4+ T cells (induced by intraperitoneal injection of the corresponding antibodies), respectively, suggesting that CD4+ T cells predominantly and CD8+ T cells partially contribute to the protective immunity in the advanced stage of influenza virus infection. These results suggest that SF-10 promotes effective antigen delivery to antigen presenting cells, activates CD8+ T cells via cross-presentation, and induces cell-mediated immune responses against antigen.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/administração & dosagem
Hemaglutinação por Vírus/imunologia
Imunidade Celular
Vacinas contra Influenza/administração & dosagem
Mimetismo Molecular
Surfactantes Pulmonares/química
[Mh] Termos MeSH secundário: Animais
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD8-Positivos/imunologia
Feminino
Vacinas contra Influenza/imunologia
Depleção Linfocítica
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Mucosa Nasal/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Influenza Vaccines); 0 (Pulmonary Surfactants)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191133


  6 / 16101 MEDLINE  
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[PMID]:28467361
[Au] Autor:Francioso A; Cossi R; Fanelli S; Mastromarino P; Mosca L
[Ad] Endereço:Department of Biochemical Sciences, "Sapienza" University of Rome, Piazzale Aldo Moro, 5, 00185 Roma, Italy. antonio.francioso@uniroma1.it.
[Ti] Título:Studies on Trans-Resveratrol/Carboxymethylated (1,3/1,6)-ß-d-Glucan Association for Aerosol Pharmaceutical Applications.
[So] Source:Int J Mol Sci;18(5), 2017 May 03.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A resveratrol/carboxymethylated glucan (CM-glucan) combination is known to inhibit rhinovirus replication and expression of inflammatory mediators in nasal epithelia. The aim of this study was to develop an aerosol formulation containing an association of the two molecules which could reach the lower respiratory tract. Mass median aerodynamic diameter (MMAD) of a resveratrol/CM-glucan combination was lower than that shown by resveratrol or CM-glucan alone (2.83 versus 3.28 and 2.96 µm, respectively). The resveratrol/CM-glucan association results in the finest and most monodispersed particles in comparison to the two single components. The association also evidenced lower values for all particle size distribution parameters, suggesting that the pharmacological synergy observed in previous studies may be accompanied by a pharmaceutical one. Moreover, we showed that the CM-glucan matrix did not exert an inhibitory effect on resveratrol nebulization, demonstrating the good suitability of these two molecules in association for simultaneous aerosol volatilization.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/química
Anti-Inflamatórios não Esteroides/farmacologia
Antivirais/química
Antivirais/farmacologia
Estilbenos/química
Estilbenos/farmacologia
beta-Glucanas/química
[Mh] Termos MeSH secundário: Aerossóis
Sobrevivência Celular/efeitos dos fármacos
Células HeLa
Seres Humanos
Mucosa Nasal
Nebulizadores e Vaporizadores
Tamanho da Partícula
Rhinovirus/efeitos dos fármacos
Volatilização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Antiviral Agents); 0 (Stilbenes); 0 (beta-Glucans); 9051-97-2 (beta-1,3-glucan); Q369O8926L (resveratrol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  7 / 16101 MEDLINE  
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[PMID]:27779422
[Au] Autor:Kouzaki H; Matsumoto K; Kikuoka H; Kato T; Tojima I; Shimizu S; Kita H; Shimizu T
[Ad] Endereço:1 Department of Otorhinolaryngology, Shiga University of Medical Science, Otsu, Shiga, Japan; and.
[Ti] Título:Endogenous Protease Inhibitors in Airway Epithelial Cells Contribute to Eosinophilic Chronic Rhinosinusitis.
[So] Source:Am J Respir Crit Care Med;195(6):737-747, 2017 Mar 15.
[Is] ISSN:1535-4970
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Cystatin A and SPINK5 are endogenous protease inhibitors (EPIs) that may play key roles in epithelial barrier function. OBJECTIVES: To investigate the roles of EPIs in the pathogenesis of chronic rhinosinusitis (CRS). METHODS: We examined the expression of cystatin A and SPINK5 in the nasal epithelial cells of patients with CRS. Additionally, the in vitro effects of recombinant EPIs on the secretion of the epithelial-derived cytokines IL-25, IL-33, and thymic stromal lymphopoietin in airway epithelial cells, and the in vivo effects of recombinant EPIs in the nasal epithelium of mice exposed to multiple airborne allergens (MAA) were examined. MEASUREMENTS AND MAIN RESULTS: Compared with control subjects and patients with noneosinophilic CRS, patients with eosinophilic CRS showed significantly lower protein and mRNA expression of cystatin A and SPINK5 in the nasal epithelium. Allergen-induced production of IL-25, IL-33, and thymic stromal lymphopoietin in normal human bronchial epithelial cells was inhibited by treatment with recombinant cystatin A or SPINK5. Conversely, the production of these cytokines was increased when cystatin A or SPINK5 were knocked down with small interfering RNA. Chronic MAA exposure induced goblet cell metaplasia and epithelial disruption in mouse nasal epithelium and decreased the tissue expression and nasal lavage levels of cystatin A and SPINK5. Intranasal instillations of recombinant EPIs attenuated this MAA-induced pathology. CONCLUSIONS: Cystatin A and SPINK5 play an important role in protecting the airway epithelium from exogenous proteases. The preservation of EPIs may have a therapeutic benefit in intractable airway inflammation, such as eosinophilic CRS.
[Mh] Termos MeSH primário: Eosinofilia/metabolismo
Células Epiteliais/metabolismo
Inibidores de Proteases/metabolismo
Rinite/metabolismo
Sinusite/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Animais
Doença Crônica
Cistatina A/metabolismo
Eosinofilia/complicações
Feminino
Seres Humanos
Interleucina-17/metabolismo
Interleucina-33/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Meia-Idade
Mucosa Nasal/metabolismo
Proteínas Secretadas Inibidoras de Proteinases/metabolismo
Rinite/complicações
Inibidor de Serinopeptidase do Tipo Kazal 5
Sinusite/complicações
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cystatin A); 0 (Interleukin-17); 0 (Interleukin-33); 0 (Protease Inhibitors); 0 (Proteinase Inhibitory Proteins, Secretory); 0 (SPINK5 protein, human); 0 (Serine Peptidase Inhibitor Kazal-Type 5)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1164/rccm.201603-0529OC


  8 / 16101 MEDLINE  
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[PMID]:29262451
[Au] Autor:Chen XQ; Ma Q; Zhou LY; Ma HA; Wu JY; Zhao JJ; Yan DN
[Ad] Endereço:Department of Otorhinolaryngology, Affiliated Hospital of Nanjing University of TCM, Nanjing 210029, China.
[Ti] Título:[Experimental study on the effect of Yiqi Wenyang Decoction on nasal mucosa infiltration of NK cells in mice with allergic rhinitis].
[So] Source:Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi;52(12):921-926, 2017 Dec 07.
[Is] ISSN:1673-0860
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To observe the effect of Yiqi Wenyang Decoction on the infiltration and activation of NK cells in nasal mucosa of mouse model with allergic rhinitis (AR), and to explore the potential mechanism for effective intervention of AR with Yiqi Wenyang Decoction. Fourty-eight mice were randomly divided into blank group, model group, low, medium and high dose of Yiqi Wenyang Decoction group and Cetirizine group, with 8 rats in each group. After modeling of AR, the model group was filled with 0.9% sodium chloride solution. Yiqi Wenyang Decoction groups of each dose were given different concentrations of Yiqi Wenyang Decoction water extract, while the Cetirizine group was given aqueous solution of Cetirizine. The behavior, morphological changes of nasal mucosa and infiltration of NK cells in nasal mucosa were observed. The levels of IL-4 and INF-γ in nasal lavage fluid were measured. Besides, the drug safety was observed by acute toxicity test. In the respect of behavioral scoring, middle and high dose of Yiqi Wenyang Decoction group were superior to the model group (number of sneezing: value was 7.189, 8.748, respectively; number of scratching nose: value was 12.074, 14.560, respectively; all <0.05). In middle and high dose of Yiqi Wenyang Decoction group, the infiltration of NK cells and nasal lavage fluid IL-4 levels were lower than those in model group (IOD: value was 10.073, 12.322, respectively; IOD/Area: value was 10.954, 14.073, respectively; IL-4: value was 4.705, 6.801, respectively; all <0.05). There was no significant difference in nasal lavage fluid of INF-γ among each group ( =1.166, >0.05). In acute toxicity test, no obvious poisoning symptoms and death occurred in mice. Yiqi Wenyang Decoction can control the nasal symptom, reduce the local NK cell infiltration of nasal mucosa and inhibit the expression of the 2-type cytokines released by NK cells, which may be related with the potential mechanism of effective intervention of AR with Yiqi Wenyang Decoction.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/uso terapêutico
Células Matadoras Naturais/efeitos dos fármacos
Rinite Alérgica/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antialérgicos/efeitos adversos
Antialérgicos/uso terapêutico
Cetirizina/efeitos adversos
Cetirizina/uso terapêutico
Modelos Animais de Doenças
Medicamentos de Ervas Chinesas/efeitos adversos
Interferon gama/análise
Interleucina-4/análise
Camundongos
Líquido da Lavagem Nasal/química
Mucosa Nasal/citologia
Mucosa Nasal/imunologia
Distribuição Aleatória
Rinite Alérgica/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Allergic Agents); 0 (Drugs, Chinese Herbal); 207137-56-2 (Interleukin-4); 82115-62-6 (Interferon-gamma); YO7261ME24 (Cetirizine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.1673-0860.2017.12.009


  9 / 16101 MEDLINE  
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[PMID]:29240797
[Au] Autor:Wang Y; Jiang S; Wang H; Bie H
[Ad] Endereço:Jining No.1 People's Hospital, Jining, Shandong Province, China.
[Ti] Título:A mucoadhesive, thermoreversible in situ nasal gel of geniposide for neurodegenerative diseases.
[So] Source:PLoS One;12(12):e0189478, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Neurodegenerative diseases are becoming prevalent as the population ages. Geniposide could inhibit oxidative stress, reduce apoptosis, protect neuron, and has been used for therapy of the neurodegenerative diseases. The bioavailability of geniposide by nasal route is greater than that by oral administration. However, mucociliary clearance is a rate-limiting factor for nasal route administration. The objective of this study was to develop and evaluate a mucoadhesive, thermoreversible in situ nasal gel of geniposide. The poloxamers (P407, P188) and the hydroxypropyl methylcellulose were used as thermoreversible and mucoadhesive polymers, respectively. Borneol was used as a permeation enhancer. The hydrogel was prepared with the cold method and optimized by the response surface methodology-central composite design. Gelation temperature, pH, clarity, gel strength, mucoadhesive strength, in vitro and ex vivo release kinetics of formulations were evaluated. The optimized amounts of poloxamer407 (P407), poloxamer188 (P188) and hydroxypropyl methylcellulose were determined to be 19.4-20.5%, 1.1-4.0% and 0.3-0.6% respectively. The second-order polynomial equation in terms of actual factors indicated a satisfactory correlation between the independent variables and the response (R2 = 0.9760). An ANOVA of the empirical second-order polynomial model indicated the model was significant (P<0.01). P407, P188, P407×P188, P4072 and P1882 were significant model terms. The effects of P407 on gelation temperature were greater than those of other independent variables. The pH values of all the formulations were found to be within 6.3-6.5 which was in the nasal physiological pH range 4.5-6.5. The drug content, gel strength, mucoadhesive strength of the optimized formulations were 97-101%, 25-50 sec and 4000-6000 dyn/cm2 respectively. The in vitro release kinetics of cumulative release of geniposide was fitted to the zero-order model. The ex vivo cumulative release kinetics of geniposide was fitted to the Weibull model. This study concludes that the release of geniposide is controlled by gel corrosion, and that the permeation of geniposide is time-dependent. The more residence time, mucoadhesive, thermoreversible in situ nasal gel of geniposide for neurodegenerative diseases is of compliance and potential application.
[Mh] Termos MeSH primário: Administração Intranasal
Géis
Iridoides/administração & dosagem
Doenças Neurodegenerativas/tratamento farmacológico
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Seres Humanos
Iridoides/metabolismo
Iridoides/farmacocinética
Iridoides/uso terapêutico
Mucosa Nasal/metabolismo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gels); 0 (Iridoids); 145295QLXY (geniposide)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189478


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[PMID]:29180288
[Au] Autor:Chalansonnet M; Carabin N; Boucard S; Merlen L; Melczer M; Antoine G; Devoy J; Remy A; Gagnaire F
[Ad] Endereço:INRS, Département Toxicologie et Biométrologie, Rue du Morvan, CS 60027, F-54519, VandÅ“uvre-lès-Nancy, France. Electronic address: monique.chalansonnet@inrs.fr.
[Ti] Título:Study of potential transfer of aluminum to the brain via the olfactory pathway.
[So] Source:Toxicol Lett;283:77-85, 2018 Feb.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Many employees in the aluminum industry are exposed to a range of aluminum compounds by inhalation, and the presence of ultrafine particles in the workplace has become a concern to occupational health professionals. Some metal salts and metal oxides have been shown to enter the brain through the olfactory route, bypassing the blood-brain barrier, but few studies have examined whether aluminum compounds also use this pathway. In this context, we sought to determine whether aluminum was found in rat olfactory bulbs and whether its transfer depended on physicochemical characteristics such as solubility and granulometry. Aluminum salts (chloride and fluoride) and various nanometric aluminum oxides (13nm, 20nm and 40-50nm) were administered to rats by intranasal instillation through one nostril (10µg Al/30µL for 10days). Olfactory bulbs (ipsilateral and contralateral relative to instilled nostril) were harvested and the aluminum content was determined by graphite furnace atomic absorption spectrometry after tissue mineralization. Some transfer of aluminum salts to the central nervous system via the olfactory route was observed, with the more soluble aluminum chloride being transferred at higher levels than aluminum fluoride. No cerebral translocation of any of the aluminas studied was detected.
[Mh] Termos MeSH primário: Compostos de Alumínio/metabolismo
Compostos de Alumínio/toxicidade
Encéfalo/metabolismo
Condutos Olfatórios/metabolismo
[Mh] Termos MeSH secundário: Administração Intranasal
Algoritmos
Compostos de Alumínio/farmacocinética
Animais
Cloretos/metabolismo
Cloretos/farmacocinética
Cloretos/toxicidade
Fluoretos/metabolismo
Fluoretos/farmacocinética
Fluoretos/toxicidade
Masculino
Mucosa Nasal/patologia
Bulbo Olfatório/metabolismo
Ratos
Ratos Sprague-Dawley
Espectrofotometria Atômica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aluminum Compounds); 0 (Chlorides); 3CYT62D3GA (aluminum chloride); Q80VPU408O (Fluorides); Z77H3IKW94 (aluminum fluoride)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE



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