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  1 / 5011 MEDLINE  
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[PMID]:29346447
[Au] Autor:Chang HJ; Shin HS; Kim TH; Yoo JY; Teasley HE; Zhao JJ; Ha UH; Jeong JW
[Ad] Endereço:Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State University, Grand Rapids, MI, United States of America.
[Ti] Título:Pik3ca is required for mouse uterine gland development and pregnancy.
[So] Source:PLoS One;13(1):e0191433, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The PI3K/AKT signaling pathway plays a critical role in the maintenance of equilibrium between cell survival and apoptosis. The Pik3ca gene is mutated in a range of human cancers. It has been found to be oncogenic, and mutations lead to constitutive activation of the PI3K/AKT pathway. The expression patterns of PIK3CA proteins in the uterus of mice during early pregnancy indicate that it may play a role in the regulation of glandular epithelial cells, which is required to support uterine receptivity. To further investigate the role of Pik3ca in uterine function, Pik3ca was conditionally ablated only in the PGR-positive cells (Pgrcre/+Pik3caf/f; Pik3cad/d). A defect of uterine gland development and decidualization led to subfertility observed in Pik3cad/d mice. Pik3cad/d mice showed significantly decreased uterine weight compared to Pik3caf/f mice. Interestingly, a significant decrease of gland numbers were detected in Pik3cad/d mice compared to control mice. In addition, we found a decrease of Foxa2 expression, which is a known uterine gland marker in Pik3cad/d mice. Furthermore, the excessive proliferation of endometrial epithelial cells was observed in Pik3cad/d mice. Our studies suggest that Pik3ca has a critical role in uterine gland development and female fertility.
[Mh] Termos MeSH primário: Fosfatidilinositol 3-Quinases/metabolismo
Útero/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Western Blotting
Proliferação Celular
Decídua/citologia
Decídua/crescimento & desenvolvimento
Implantação do Embrião
Feminino
Fertilidade
Camundongos
Camundongos Endogâmicos C57BL
Mutação
Fosfatidilinositol 3-Quinases/genética
Gravidez
Reação em Cadeia da Polimerase em Tempo Real
Útero/citologia
Útero/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.1.137 (Pik3ca protein, mouse)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191433


  2 / 5011 MEDLINE  
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[PMID]:29324807
[Au] Autor:Wheeler KC; Jena MK; Pradhan BS; Nayak N; Das S; Hsu CD; Wheeler DS; Chen K; Nayak NR
[Ad] Endereço:Department of Obstetrics and Gynecology, Wayne State University, Detroit, Michigan, United States of America.
[Ti] Título:VEGF may contribute to macrophage recruitment and M2 polarization in the decidua.
[So] Source:PLoS One;13(1):e0191040, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:It is increasingly evident that cytokines and growth factors produced in the decidua play a pivotal role in the regulation of the local immune microenvironment and the establishment of pregnancy. One of the major growth factors produced in the decidua is vascular endothelial growth factor (VEGF), which acts not only on endothelial cells, but also on multiple other cell types, including macrophages. We sought to determine whether decidua-derived VEGF affects macrophage recruitment and polarization using human endometrial/decidual tissue samples, primary human endometrial stromal cells (ESCs), and the human monocyte cell line THP1. In situ hybridization was used for assessment of local VEGF expression and immunohistochemistry was used for identification and localization of CD68-positive endometrial macrophages. Macrophage migration in culture was assessed using a transwell migration assay, and the various M1/M2 phenotypic markers and VEGF expression were assessed using quantitative real-time PCR (qRT-PCR). We found dramatic increases in both VEGF levels and macrophage numbers in the decidua during early pregnancy compared to the secretory phase endometrium (non-pregnant), with a significant increase in M2 macrophage markers, suggesting that M2 is the predominant macrophage phenotype in the decidua. However, decidual samples from preeclamptic pregnancies showed a significant shift in macrophage phenotype markers, with upregulation of M1 and downregulation of M2 markers. In THP1 cultures, VEGF treatment significantly enhanced macrophage migration and induced M1 macrophages to shift to an M2 phenotype. Moreover, treatment with conditioned media from decidualized ESCs induced changes in macrophage migration and polarization similar to that of VEGF treatment. These effects were abrogated by the addition of a potent VEGF inhibitor. Together these results suggest that decidual VEGF plays a significant role in macrophage recruitment and M2 polarization, and that inhibition of VEGF signaling may contribute to the shift in macrophage polarity observed in different pregnancy disorders, including preeclampsia.
[Mh] Termos MeSH primário: Polaridade Celular
Decídua/citologia
Macrófagos/citologia
Fator A de Crescimento do Endotélio Vascular/fisiologia
[Mh] Termos MeSH secundário: Adulto
Linhagem Celular
Feminino
Seres Humanos
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Vascular Endothelial Growth Factor A)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191040


  3 / 5011 MEDLINE  
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[PMID]:29308859
[Au] Autor:Radovic Janosevic D; Popovic J; Krstic M; Tubic-Pavlovic A; Stefanovic M; Pop-Trajkovic S
[Ti] Título:The structure of immunocompetent decidual cells in recurrent missed abortions.
[So] Source:Vojnosanit Pregl;73(4):306-11, 2016 Apr.
[Is] ISSN:0042-8450
[Cp] País de publicação:Serbia
[La] Idioma:eng
[Ab] Resumo:Background/Aim: Recurrent or habitual missed abortions (RMA) are defined as three or more consecutive abortions. In the first trimester of pregnancy habitual missed abortions occur in about 1% of population. The aim of this immunohistochemical study of decidua in RMA of unknown etiology was to identify subpopulations of decidual lymphocytes in recurrent miscarriages and compare the distribution of immunocompetent cells in artificial abortions and RMA. Methods. The study included 30 women with at least 2 consecutive miscarriages in the first trimester of pregnancy. Curettements of the third missed abortion were immunohistochemically analyzed. The control group consisted of 20 women without loaded reproductive anamnesis, with the abortion for social reasons. Criteria for exclusion from the study were diagnosed uterine anomalies, positive screening for thrombophilia and women who suffered from diabetes mellitus and disorders in the function of the thyroid gland. Immunophenotyping was performed by immuno-alkaline phosphatase (APAAP) using monoclonal antibodies: CD 30, CD 45 RO, CD 56 and CD 57, CD 68. Methods: The number of missed abortions (1,223) was on the average 9.7% of all deliveriies during the test period. Among them RMA were registered in 52 (4.2%) patients and in 30 (57%) the exact etiology of abortions was not determined. RMA was most common in the 25-34 years of age group. The largest number of RMA showed the ultrasound characteristics of missed abortion in 60% of cases and was in nulliparous patients (76.7%). The number of NK CD56 positive cells did not differ significantly between the types of abortion. In the decidual tissue, a number of NK CD57 positive cells was significantly higher in missed abortions compared to artificial interruptions (p < 0.01). In artificial termination of pregnancy there was an absolute predominance of CD45RO lymphocyte subpopulations, whereas in the RMA group there was slightly greater predominance of CD30 positive cells. The completed analysis showed a significantly higher number of CD68 positive macrophages in a decidual tissue of RMA pregnancy (p < 0.01). Results: The number and phenotypic structure of NK cells are significantly different in normal pregnancy decidua and in RMA. The NK cell dominance is present in the RMA group, in favor of CD56+ and CD 57 of subpopulations with increased CD30 of T lymphocyte subpopulations. Macrophages are more numerous in the decidua of pregnancies ended in abortion, so the cause to RMA of unknown etiology in a number of cases could be disregulation of immunocompetent cells.
[Mh] Termos MeSH primário: Aborto Retido/imunologia
Decídua/imunologia
Decídua/metabolismo
Células Matadoras Naturais/imunologia
[Mh] Termos MeSH secundário: Aborto Retido/metabolismo
Adulto
Antígeno CD56
Antígenos CD57
Feminino
Seres Humanos
Imuno-Histoquímica
Imunofenotipagem
Antígeno Ki-1
Células Matadoras Naturais/classificação
Células Matadoras Naturais/metabolismo
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD56 Antigen); 0 (CD57 Antigens); 0 (Ki-1 Antigen); 0 (NCAM1 protein, human)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE
[do] DOI:10.2298/VSP141226018R


  4 / 5011 MEDLINE  
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[PMID]:29262349
[Au] Autor:Fu B; Zhou Y; Ni X; Tong X; Xu X; Dong Z; Sun R; Tian Z; Wei H
[Ad] Endereço:Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Science and Medical Center, University of Science and Technology of China, Hefei, Anhui 230001, China; Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Te
[Ti] Título:Natural Killer Cells Promote Fetal Development through the Secretion of Growth-Promoting Factors.
[So] Source:Immunity;47(6):1100-1113.e6, 2017 Dec 19.
[Is] ISSN:1097-4180
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Natural killer (NK) cells are present in large populations at the maternal-fetal interface during early pregnancy. However, the role of NK cells in fetal growth is unclear. Here, we have identified a CD49a Eomes subset of NK cells that secreted growth-promoting factors (GPFs), including pleiotrophin and osteoglycin, in both humans and mice. The crosstalk between HLA-G and ILT2 served as a stimulus for GPF-secreting function of this NK cell subset. Decreases in this GPF-secreting NK cell subset impaired fetal development, resulting in fetal growth restriction. The transcription factor Nfil3, but not T-bet, affected the function and the number of this decidual NK cell subset. Adoptive transfer of induced CD49a Eomes NK cells reversed impaired fetal growth and rebuilt an appropriate local microenvironment. These findings reveal properties of NK cells in promoting fetal growth. In addition, this research proposes approaches for therapeutic administration of NK cells in order to reverse restricted nourishments within the uterine microenvironment during early pregnancy.
[Mh] Termos MeSH primário: Aborto Habitual/imunologia
Transferência Adotiva
Proteínas de Transporte/secreção
Citocinas/secreção
Desenvolvimento Fetal/imunologia
Retardo do Crescimento Fetal/prevenção & controle
Peptídeos e Proteínas de Sinalização Intercelular/secreção
Células Matadoras Naturais/transplante
[Mh] Termos MeSH secundário: Aborto Habitual/genética
Aborto Habitual/patologia
Adulto
Animais
Antígenos CD/genética
Antígenos CD/imunologia
Fatores de Transcrição de Zíper de Leucina Básica/genética
Fatores de Transcrição de Zíper de Leucina Básica/imunologia
Proteínas de Transporte/genética
Proteínas de Transporte/imunologia
Microambiente Celular
Citocinas/genética
Citocinas/imunologia
Decídua/imunologia
Decídua/patologia
Feminino
Retardo do Crescimento Fetal/genética
Retardo do Crescimento Fetal/imunologia
Retardo do Crescimento Fetal/patologia
Feto
Regulação da Expressão Gênica no Desenvolvimento
Antígenos HLA-G/genética
Antígenos HLA-G/imunologia
Seres Humanos
Integrina alfa1/genética
Integrina alfa1/imunologia
Peptídeos e Proteínas de Sinalização Intercelular/genética
Peptídeos e Proteínas de Sinalização Intercelular/imunologia
Células Matadoras Naturais/citologia
Células Matadoras Naturais/imunologia
Receptor B1 de Leucócitos Semelhante a Imunoglobulina/genética
Receptor B1 de Leucócitos Semelhante a Imunoglobulina/imunologia
Camundongos
Camundongos Endogâmicos C57BL
Gravidez
Transdução de Sinais
Proteínas com Domínio T-Box/genética
Proteínas com Domínio T-Box/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Basic-Leucine Zipper Transcription Factors); 0 (Carrier Proteins); 0 (Cytokines); 0 (EOMES protein, human); 0 (HLA-G Antigens); 0 (Integrin alpha1); 0 (Intercellular Signaling Peptides and Proteins); 0 (LILRB1 protein, human); 0 (Leukocyte Immunoglobulin-like Receptor B1); 0 (NFIL3 protein, human); 0 (OGN protein, human); 0 (T-Box Domain Proteins); 0 (T-box transcription factor TBX21); 134034-50-7 (pleiotrophin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE


  5 / 5011 MEDLINE  
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[PMID]:28657144
[Au] Autor:Yu HF; Tao R; Yang ZQ; Wang K; Yue ZP; Guo B
[Ad] Endereço:College of Veterinary Medicine, Jilin University, Changchun, P.R. China.
[Ti] Título:Ptn functions downstream of C/EBPß to mediate the effects of cAMP on uterine stromal cell differentiation through targeting Hand2 in response to progesterone.
[So] Source:J Cell Physiol;233(2):1612-1626, 2018 Feb.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ptn is a pleiotropic growth factor involving in the regulation of cellular proliferation and differentiation, but its biological function in uterine decidualization remains unknown. Here, we showed that Ptn was highly expressed in the decidual cells, and could induce the proliferation of uterine stromal cells and expression of Prl8a2 and Prl3c1 which were two well-established differentiation markers for decidualization, suggesting an important role of Ptn in decidualization. In the uterine stromal cells, progesterone stimulated the expression of Ptn accompanied with an accumulation of intracellular cAMP level. Silencing of Ptn impeded the induction of progesterone and cAMP on the differentiation of uterine stromal cells. Administration of PKA inhibitor H89 resulted in a blockage of progesterone on Ptn expression. Further analysis evidenced that regulation of progesterone and cAMP on Ptn was mediated by C/EBPß. During in vitro decidualization, knockdown of Ptn could weaken the up-regulation of Prl8a2 and Prl3c1 elicited by C/EBPß overexpression, while constitutive activation of Ptn reversed the repressive effects of C/EBPß siRNA on the expression of Prl8a2 and Prl3c1. Meanwhile, Ptn might mediate the regulation of C/EBPß on Hand2 which was a downstream target of Ptn in the differentiation of uterine stromal cells. Attenuation of Ptn or C/EBPß by specific siRNA blocked the stimulation of Hand2 by progesterone and cAMP. Collectively, Ptn may play a vital role in the progesterone-induced decidualization pathway.
[Mh] Termos MeSH primário: 8-Bromo Monofosfato de Adenosina Cíclica/farmacologia
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Proteína beta Intensificadora de Ligação a CCAAT/metabolismo
Proteínas de Transporte/metabolismo
Diferenciação Celular/efeitos dos fármacos
Citocinas/metabolismo
Decídua/efeitos dos fármacos
Progesterona/farmacologia
Células Estromais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Proteína beta Intensificadora de Ligação a CCAAT/genética
Proteínas de Transporte/genética
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
Citocinas/genética
Decídua/citologia
Decídua/metabolismo
Feminino
Regulação da Expressão Gênica
Camundongos
Gravidez
Prolactina/análogos & derivados
Prolactina/genética
Prolactina/metabolismo
Interferência de RNA
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Transdução de Sinais/efeitos dos fármacos
Células Estromais/metabolismo
Fatores de Tempo
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Basic Helix-Loop-Helix Transcription Factors); 0 (CCAAT-Enhancer-Binding Protein-beta); 0 (Carrier Proteins); 0 (Cebpb protein, mouse); 0 (Cytokines); 0 (Dtprp protein, mouse); 0 (Hand2 protein, mouse); 0 (RNA, Messenger); 134034-50-7 (pleiotrophin); 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate); 4G7DS2Q64Y (Progesterone); 9002-62-4 (Prolactin); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.26067


  6 / 5011 MEDLINE  
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[PMID]:28977591
[Au] Autor:Tamura I; Shirafuta Y; Jozaki K; Kajimura T; Shinagawa M; Maekawa R; Taketani T; Asada H; Sato S; Tamura H; Sugino N
[Ad] Endereço:Department of Obstetrics and Gynecology, Yamaguchi University Graduate School of Medicine, Minamikogushi 1-1-1, Ube 755-8505, Japan.
[Ti] Título:Novel Function of a Transcription Factor WT1 in Regulating Decidualization in Human Endometrial Stromal Cells and Its Molecular Mechanism.
[So] Source:Endocrinology;158(10):3696-3707, 2017 Oct 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Wilms tumor suppressor gene (WT1) encodes an essential transcription factor regulating mammalian urogenital development. However, the function of WT1 in human endometrium is still unclear. The current study examined the involvement of WT1 in the regulation of IGF-binding protein-1 (IGFBP-1) and prolactin (PRL), which are specific markers of decidualization, in human endometrial stromal cells (ESCs) undergoing decidualization. ESCs isolated from proliferative-phase endometrium were incubated with cyclic adenosine monophosphate (cAMP) to induce decidualization. cAMP increased WT1 expression with the induction of IGFBP-1 and PRL. Knockdown of WT1 by small interfering RNA inhibited cAMP-induced expression of IGFBP-1 and PRL. cAMP also induced the recruitment of WT1 to the IGFBP-1 and PRL promoters. To investigate the mechanism by which WT1 is upregulated by cAMP, we focused on C/EBPß, a gene that regulates the expression of many genes during decidualization. Knockdown of C/EBPß decreased cAMP-increased WT1 expression. cAMP increased the recruitment of C/EBPß to the WT1 enhancer that is located approximately 14,000 bp downstream from the transcription start site. To test the endogenous function of the WT1 enhancer region on WT1 expression, the endogenous WT1 enhancer region was deleted by CRISPR/Cas9 system in HEK293 cells. The increase of WT1 expression by cAMP was not observed in the enhancer-deleted clones. Chromatin immunoprecipitation assay revealed that this enhancer region has high levels of H3K27ac and H3K4me1, which are active enhancer marks. These results show the role of WT1 in regulating decidualization in human ESCs. C/EBPß is an upstream gene that regulates WT1 expression by binding to the novel enhancer region.
[Mh] Termos MeSH primário: Proteína beta Intensificadora de Ligação a CCAAT/genética
AMP Cíclico/metabolismo
Decídua/metabolismo
Proteína 1 de Ligação a Fator de Crescimento Semelhante à Insulina/metabolismo
Prolactina/metabolismo
Células Estromais/metabolismo
Proteínas WT1/genética
[Mh] Termos MeSH secundário: Proteína beta Intensificadora de Ligação a CCAAT/metabolismo
Sistemas CRISPR-Cas
Imunoprecipitação da Cromatina
Decídua/citologia
Endométrio/citologia
Endométrio/metabolismo
Elementos Facilitadores Genéticos
Feminino
Técnicas de Silenciamento de Genes
Células HEK293
Histonas/metabolismo
Seres Humanos
Proteínas WT1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CCAAT-Enhancer-Binding Protein-beta); 0 (CEBPB protein, human); 0 (Histones); 0 (IGFBP1 protein, human); 0 (Insulin-Like Growth Factor Binding Protein 1); 0 (WT1 Proteins); 0 (WT1 protein, human); 9002-62-4 (Prolactin); E0399OZS9N (Cyclic AMP)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-00478


  7 / 5011 MEDLINE  
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[PMID]:28911927
[Au] Autor:Salsano S; Quiñonero A; Pérez S; Garrido Gómez T; Simón C; Dominguez F
[Ad] Endereço:Fundación Instituto Valenciano de Infertilidad (FIVI), Instituto Universitario IVI (IUIVI), Valencia, Spain.
[Ti] Título:Dynamic expression of PGRMC1 and SERBP1 in human endometrium: an implication in the human decidualization process.
[So] Source:Fertil Steril;108(5):832-842.e1, 2017 Nov.
[Is] ISSN:1556-5653
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To characterize PGRMC1 and SERBP1 in human endometrium and to investigate the putative role of PGRMC1 in endometrial decidualization. DESIGN: The PGRMC1 and SERBP1 expression in human endometrium was determined throughout the menstrual cycle. We analyzed the colocalization of PGRMC1 and SERBP1. Then, endometrial stromal cells (ESCs) were isolated to investigate the functional effect of PGRMC1 overexpression on decidualization. SETTING: IVI clinic. PATIENT(S): Endometrial biopsies were collected from fertile volunteers (n = 61) attending the clinic as ovum donors. INTERVENTION(S): Endometrial samples of 61 healthy fertile women. MAIN OUTCOME MEASURE(S): In vivo localization of PGRMC1 and SERBP1 was assessed by immunohistochemistry. The PGRMC1/SERBP1 colocalization was investigated in vitro and in vivo. Decidualization effect of PGRMC1 overexpression was evaluated in primary ESC cultures. RESULT(S): The PGRMC1 was detected in the endometrial stroma throughout the menstrual cycle, but decreased in the late secretory phase. The SERBP1 immunostaining was present in stroma and increased in the entire the menstrual cycle. The PGRMC1 and SERBP1 colocalized in the cytoplasmic fractions of nondecidualized and decidualized ESC. The PGRMC1 overexpression significantly inhibited in vitro decidualization. CONCLUSION(S): Our results suggest that classic P receptors (PRs) are not the only kind playing a role in the normal physiology of the endometrium. The human decidualization process could be altered by the overexpression or mislocalization of PGRMC1 in ESC.
[Mh] Termos MeSH primário: Decídua/metabolismo
Endométrio/metabolismo
Proteínas de Membrana/metabolismo
Ciclo Menstrual/metabolismo
Proteínas de Ligação a RNA/metabolismo
Receptores de Progesterona/metabolismo
Células Estromais/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Células Cultivadas
Decídua/citologia
Endométrio/citologia
Feminino
Voluntários Saudáveis
Seres Humanos
Fatores de Tempo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membrane Proteins); 0 (PGRMC1 protein, human); 0 (RNA-Binding Proteins); 0 (Receptors, Progesterone); 0 (SERBP1 protein, human)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE


  8 / 5011 MEDLINE  
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[PMID]:28902929
[Au] Autor:Li N; Wu HM; Hang F; Zhang YS; Li MJ
[Ad] Endereço:Department of Reproductive Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
[Ti] Título:Women with recurrent spontaneous abortion have decreased 25(OH) vitamin D and VDR at the fetal-maternal interface.
[So] Source:Braz J Med Biol Res;50(11):e6527, 2017 Sep 12.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Immunological mechanisms have been proposed to underlie the pathogenesis of recurrent spontaneous abortion (RSA). Vitamin D has a potent immunomodulatory effect, which may affect pregnancy outcome. The objective of this study was to investigate 25-hydroxyvitamin D [25(OH) D] concentration and vitamin D receptor (VDR) expression in the decidual tissues of RSA patients. Thirty women with RSA (RSA group) and thirty women undergoing elective abortion (control group) were recruited during 2016 from gynecology outpatient clinics. We measured 25(OH) D, interleukin (IL)-17, IL-23, transforming growth factor ß (TGF-ß), VDR and 1-α-hydroxylase (CYP27B1) in decidual tissues collected during the abortion procedure. In the RSA group, 25(OH) D and TGF-ß were significantly decreased while IL-17 and IL-23 were significantly increased compared with the control group. VDR expression was significantly decreased in the RSA group compared with the control group. Logistic regression analysis showed a significant negative correlation between 25(OH) D in decidual tissues and RSA. These results indicated that vitamin D concentrations in the decidua are associated with inflammatory cytokine production, suggesting that vitamin D and VDR may play a role in the etiology of RSA.
[Mh] Termos MeSH primário: 25-Hidroxivitamina D3 1-alfa-Hidroxilase/análise
Aborto Habitual/metabolismo
Decídua/química
Receptores de Calcitriol/análise
Vitamina D/análogos & derivados
[Mh] Termos MeSH secundário: 25-Hidroxivitamina D3 1-alfa-Hidroxilase/metabolismo
Aborto Habitual/etiologia
Adulto
Feminino
Seres Humanos
Interleucina-17/análise
Interleucina-23/análise
Modelos Logísticos
Gravidez
Terceiro Trimestre da Gravidez
Receptores de Calcitriol/metabolismo
Fatores de Risco
Estatísticas não Paramétricas
Fator de Crescimento Transformador beta/análise
Vitamina D/análise
Vitamina D/metabolismo
Deficiência de Vitamina D/complicações
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-17); 0 (Interleukin-23); 0 (Receptors, Calcitriol); 0 (Transforming Growth Factor beta); 1406-16-2 (Vitamin D); 64719-49-9 (25-hydroxyvitamin D); EC 1.14.13.13 (25-Hydroxyvitamin D3 1-alpha-Hydroxylase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE


  9 / 5011 MEDLINE  
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[PMID]:28751217
[Au] Autor:Laurent L; Deroy K; St-Pierre J; Côté F; Sanderson JT; Vaillancourt C
[Ad] Endereço:INRS-Institut Armand-Frappier, Laval, QC, Canada; BioMed Research Centre, Laval, QC, Canada; Center for Interdisciplinary Research on Well-Being, Health, Society and Environment, Université du Québec à Montréal, Montréal, QC, Canada.
[Ti] Título:Human placenta expresses both peripheral and neuronal isoform of tryptophan hydroxylase.
[So] Source:Biochimie;140:159-165, 2017 Sep.
[Is] ISSN:1638-6183
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The role of placental serotonin has been an active topic of research notably because of its crucial role in brain development. However, which cell types synthesize serotonin in human placenta remains unknown. Moreover, it is not known if the two tryptophan hydroxylase isoforms (TPH1 and TPH2), the rate-limiting enzymes in serotonin biosynthesis, are expressed in placenta. Human placentas were obtained in first trimester or at term, and trophoblast cells were isolated and purified using a magnetic cell sorter and placed in primary culture. The tissue sublocalization of each TPH was determined by immunohistochemistry. TPH expression in primary villous trophoblasts was determined by PCR and immunoblotting, and serotonin secretion by LC-MS/MS. Villous cytotrophoblasts, syncytiotrophoblast, fetal capillaries, extravillous cytotrophoblasts, and decidual cells co-expressed TPH1 and TPH2. Moreover, mRNA and protein levels of both TPHs were detected in human primary trophoblast as well as in mouse placental tissues. Finally, human trophoblast cells were shown to produce serotonin de novo. This study demonstrates that both TPH1 and TPH2 are expressed in human and mouse placenta throughout pregnancy and helps to better understand the placental serotonin system, which is crucial for healthy pregnancy and fetal development. It is therefore important to further understand regulation of the placental serotonin system and how its disruption during pregnancy may impact the developing fetus and subsequent child programming.
[Mh] Termos MeSH primário: Decídua/enzimologia
Regulação Enzimológica da Expressão Gênica/fisiologia
Proteínas da Gravidez/biossíntese
Trofoblastos/enzimologia
Triptofano Hidroxilase/biossíntese
[Mh] Termos MeSH secundário: Animais
Decídua/citologia
Feminino
Seres Humanos
Isoenzimas/biossíntese
Camundongos
Gravidez
Serotonina/biossíntese
Trofoblastos/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isoenzymes); 0 (Pregnancy Proteins); 333DO1RDJY (Serotonin); EC 1.14.16.4 (TPH1 protein, human); EC 1.14.16.4 (TPH2 protein, human); EC 1.14.16.4 (Tryptophan Hydroxylase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE


  10 / 5011 MEDLINE  
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[PMID]:28588064
[Au] Autor:Aikawa S; Kano K; Inoue A; Wang J; Saigusa D; Nagamatsu T; Hirota Y; Fujii T; Tsuchiya S; Taketomi Y; Sugimoto Y; Murakami M; Arita M; Kurano M; Ikeda H; Yatomi Y; Chun J; Aoki J
[Ad] Endereço:Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, Japan.
[Ti] Título:Autotaxin-lysophosphatidic acid-LPA signaling at the embryo-epithelial boundary controls decidualization pathways.
[So] Source:EMBO J;36(14):2146-2160, 2017 Jul 14.
[Is] ISSN:1460-2075
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:During pregnancy, up-regulation of heparin-binding (HB-) EGF and cyclooxygenase-2 (COX-2) in the uterine epithelium contributes to decidualization, a series of uterine morphological changes required for placental formation and fetal development. Here, we report a key role for the lipid mediator lysophosphatidic acid (LPA) in decidualization, acting through its G-protein-coupled receptor LPA in the uterine epithelium. Knockout of or inhibition of the LPA-producing enzyme autotaxin (ATX) in pregnant mice leads to HB-EGF and COX-2 down-regulation near embryos and attenuates decidual reactions. Conversely, selective pharmacological activation of LPA induces decidualization via up-regulation of HB-EGF and COX-2. ATX and its substrate lysophosphatidylcholine can be detected in the uterine epithelium and in pre-implantation-stage embryos, respectively. Our results indicate that ATX-LPA-LPA signaling at the embryo-epithelial boundary induces decidualization via the canonical HB-EGF and COX-2 pathways.
[Mh] Termos MeSH primário: Decídua/crescimento & desenvolvimento
Embrião de Mamíferos/fisiologia
Lisofosfolipídeos/metabolismo
Diester Fosfórico Hidrolases/metabolismo
Receptores de Ácidos Lisofosfatídicos/metabolismo
Transdução de Sinais
Útero/fisiologia
[Mh] Termos MeSH secundário: Animais
Ciclo-Oxigenase 2/metabolismo
Desenvolvimento Embrionário
Feminino
Técnicas de Inativação de Genes
Fator de Crescimento Semelhante a EGF de Ligação à Heparina/metabolismo
Camundongos
Camundongos Knockout
Receptores de Ácidos Lisofosfatídicos/deficiência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hbegf protein, mouse); 0 (Heparin-binding EGF-like Growth Factor); 0 (Lpar3 protein, mouse); 0 (Lysophospholipids); 0 (Receptors, Lysophosphatidic Acid); EC 1.14.99.- (Ptgs2 protein, mouse); EC 1.14.99.1 (Cyclooxygenase 2); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.1.4.39 (alkylglycerophosphoethanolamine phosphodiesterase); PG6M3969SG (lysophosphatidic acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170727
[Lr] Data última revisão:
170727
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.15252/embj.201696290



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