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  1 / 59119 MEDLINE  
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[PMID]:29385186
[Au] Autor:Eladak S; Moison D; Guerquin MJ; Matilionyte G; Kilcoyne K; N'Tumba-Byn T; Messiaen S; Deceuninck Y; Pozzi-Gaudin S; Benachi A; Livera G; Antignac JP; Mitchell R; Rouiller-Fabre V; Habert R
[Ad] Endereço:Univ. Paris Diderot, Sorbonne Paris Cité, Laboratory of Development of the Gonads, Unit of Genetic Stability, Stem Cells and Radiation, Fontenay-aux-Roses, France.
[Ti] Título:Effects of environmental Bisphenol A exposures on germ cell development and Leydig cell function in the human fetal testis.
[So] Source:PLoS One;13(1):e0191934, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Using an organotypic culture system termed human Fetal Testis Assay (hFeTA) we previously showed that 0.01 µM BPA decreases basal, but not LH-stimulated, testosterone secreted by the first trimester human fetal testis. The present study was conducted to determine the potential for a long-term antiandrogenic effect of BPA using a xenograft model, and also to study the effect of BPA on germ cell development using both the hFETA and xenograft models. METHODS: Using the hFeTA system, first trimester testes were cultured for 3 days with 0.01 to 10 µM BPA. For xenografts, adult castrate male nude mice were injected with hCG and grafted with first trimester testes. Host mice received 10 µM BPA (~ 500 µg/kg/day) in their drinking water for 5 weeks. Plasma levels of total and unconjugated BPA were 0.10 µM and 0.038 µM respectively. Mice grafted with second trimester testes received 0.5 and 50 µg/kg/day BPA by oral gavage for 5 weeks. RESULTS: With first trimester human testes, using the hFeTA model, 10 µM BPA increased germ cell apoptosis. In xenografts, germ cell density was also reduced by BPA exposure. Importantly, BPA exposure significantly decreased the percentage of germ cells expressing the pluripotency marker AP-2γ, whilst the percentage of those expressing the pre-spermatogonial marker MAGE-A4 significantly increased. BPA exposure did not affect hCG-stimulated androgen production in first and second trimester xenografts as evaluated by both plasma testosterone level and seminal vesicle weight in host mice. CONCLUSIONS: Exposure to BPA at environmentally relevant concentrations impairs germ cell development in first trimester human fetal testis, whilst gonadotrophin-stimulated testosterone production was unaffected in both first and second trimester testis. Studies using first trimester human fetal testis demonstrate the complementarity of the FeTA and xenograft models for determining the respective short-term and long term effects of environmental exposures.
[Mh] Termos MeSH primário: Compostos Benzidrílicos/toxicidade
Poluentes Ambientais/toxicidade
Células Intersticiais do Testículo/efeitos dos fármacos
Fenóis/toxicidade
Espermatozoides/efeitos dos fármacos
Testículo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Feminino
Xenoenxertos
Seres Humanos
Masculino
Camundongos
Camundongos Nus
Gravidez
Primeiro Trimestre da Gravidez
Segundo Trimestre da Gravidez
Radioimunoensaio
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Testículo/citologia
Testículo/embriologia
Testosterona/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzhydryl Compounds); 0 (Environmental Pollutants); 0 (Phenols); 3XMK78S47O (Testosterone); MLT3645I99 (bisphenol A)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191934


  2 / 59119 MEDLINE  
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[PMID]:29277915
[Au] Autor:Zhang X; Ping HY; Li JH; Duan SX; Jiang XW
[Ad] Endereço:Department of Pediatric Surgery, The Affiliated Maternal and Child Health Hospital of Shenzhen University Medical College, Shenzhen, China.
[Ti] Título:Diethylstilbestrol regulates mouse gubernaculum testis cell proliferation via PLC-Ca -CREB pathway.
[So] Source:Cell Biochem Funct;36(1):13-17, 2018 Jan.
[Is] ISSN:1099-0844
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recent evidence suggested a positive correlation between environmental estrogens (EEs) and high incidence of abnormalities in male urogenital system, but the mechanism remains unclear. Diethylstilbestrol (DES) is a nonsteroidal synthetic estrogen that disrupts the morphology and proliferation of gubernaculum testis cells, but the underlying mechanism is unclear. In this study, mouse gubernaculum testis cells were pretreated with phospholipase C (PLC) inhibitor U-73122 and then treated with DES. The results demonstrated that U-73122 impaired DES-evoked intracellular Ca2+ mobilization in gubernaculum testis cells and inhibited DES-induced proliferation of gubernaculum testis cells. Mechanistically, we found that U-73122 inhibited DES-induced activation of cAMP-response element binding protein (CREB) in gubernaculum testis cells. In conclusion, these data suggest that the effects of DES on mouse gubernaculum testis cells are mediated by PLC-Ca -CREB pathway. SIGNIFICANCE OF THE STUDY: Environmental estrogens remain a serious threat to male reproductive health, and it is important to understand the mechanism by which EEs affect the male productive system. Here we explore potential mechanisms how the proliferation and contractility of gubernaculum testis cells are regulated by diethylstilbestrol. Our findings provide the first evidence that PLC-Ca -CREB signalling pathway mediates the nongenomic effects of diethylstilbestrol on gubernaculum testis cells. These findings provide new insight into the role of diethylstilbestrol in the aetiology of male reproductive dysfunction and will help develop better approaches for the prevention and therapy of male reproductive malformation.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo
Dietilestilbestrol/farmacologia
Gubernáculo/efeitos dos fármacos
Testículo/efeitos dos fármacos
Fosfolipases Tipo C/metabolismo
[Mh] Termos MeSH secundário: Animais
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Estrenos/farmacologia
Gubernáculo/citologia
Gubernáculo/metabolismo
Masculino
Camundongos
Pirrolidinonas/farmacologia
Testículo/citologia
Testículo/metabolismo
Fosfolipases Tipo C/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclic AMP Response Element-Binding Protein); 0 (Estrenes); 0 (Pyrrolidinones); 112648-68-7 (1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione); 731DCA35BT (Diethylstilbestrol); EC 3.1.4.- (Type C Phospholipases); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171227
[St] Status:MEDLINE
[do] DOI:10.1002/cbf.3312


  3 / 59119 MEDLINE  
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[PMID]:28450650
[Au] Autor:Birowo P; Putra DE; Dewi M; Rasyid N; Taher A
[Ad] Endereço:Department of Urology, Faculty of medicine Universitas Indonesia - Cipto Mangunkusumo Hospital, Jakarta, Indonesia. ponco.birowo@gmail.com.
[Ti] Título:Y-Chromosomal Microdeletion in Idiopathic Azoospermic and Severe Oligozoospermic Indonesian Men.
[So] Source:Acta Med Indones;49(1):17-23, 2017 Jan.
[Is] ISSN:0125-9326
[Cp] País de publicação:Indonesia
[La] Idioma:eng
[Ab] Resumo:AIM: to detect Y-chromosomal microdeletion in Indonesian men with azoospermia or severe oligozoospermia using multiplex PCR. METHODS: we performed 2 multiplex PCR amplifications of the Azoospermia Factor (AZF) region in 71 men. Criteria for including a patient were fulfilled if they presented with azoospermia or severe oligozoospermia, with or without additional abnormalities of sperm motility or of head morphology, raised or normal levels of FSH, normal levels of LH and testosterone, and with no evidence of testicular tumors or other abnormalities. Five men participated as control persons. RESULTS: partial deletion of AZFa was found in 11 men (15.49%), complete deletion of AZFb in 1 man (1.4%), and complete deletion of AZFc in 1 man (1.4%). The unspecific type of deletion was also detected, including the DBY gene in 2 men (2.81%), and partial deletion of both AZFa and AZFb in 2 men (2.81%). No AZF deletion was observed in the control probands. Related to the type of deletion, the AZFa and AZFb deletion showed spermatogenesis arrest in most tubules, while deletion of the DBY gene is associated with the sertoli cell only (SCO) syndrome. CONCLUSION: the frequency of partial deletion of AZFa was found to be relatively high in our center. The type of deletion is associated with the testicular histology.
[Mh] Termos MeSH primário: Azoospermia/genética
Infertilidade Masculina/genética
Oligospermia/genética
Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/genética
Testículo/patologia
[Mh] Termos MeSH secundário: Adulto
Grupo com Ancestrais do Continente Asiático/genética
Deleção Cromossômica
Cromossomos Humanos Y/genética
Seres Humanos
Indonésia
Masculino
Reação em Cadeia da Polimerase Multiplex
Aberrações dos Cromossomos Sexuais
Espermatozoides/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  4 / 59119 MEDLINE  
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[PMID]:29478635
[Au] Autor:Chen J; Meng T; Li Y; Gao K; Qin Z
[Ad] Endereço:State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: 13070171175@163.com.
[Ti] Título:Effects of triclosan on gonadal differentiation and development in the frog Pelophylax nigromaculatus.
[So] Source:J Environ Sci (China);64:157-165, 2018 Feb.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Previous studies have reported that triclosan (TCS) could possess an androgenic activity. We aimed to investigate the effects of TCS on gonadal differentiation and development in the frog Pelophylax nigromaculatus, a sensitive amphibian species to androgenic chemicals. P. nigromaculatus tadpoles at stage 24 were exposed to TCS (3, 30, and 300nmol/L) to stage 46 in a semi-static exposure system. At the end of exposure, gonadal morphology and histology, sex ratio and gonadal expression of sex-biased genes were examined in P. nigromaculatus. In each TCS treatment group, we found several individuals whose gonads exhibited morphological and/or histological abnormalities. Gonadal histological abnormalities were characterized by few oocytes and many somatic cells. Although the percentage of the individuals with abnormal gonads was low (7.8%) among all animals treated with TCS, statistical test revealed the sex ratios in the 3 and 300nmol/L TCS treatment groups were significantly different from the solvent control. In the 30nmol/L TCS treatment group, abnormal gonads were also observed, although the sex ratio was not changed compared with the solvent control, which was possibly due to the smaller sample size in this group. In all the TCS treatment groups, the sex ratios were not obviously male-biased, but the expression levels of some sex-biased genes were significantly altered by TCS. Altogether, our results suggest that TCS, even at environmentally relevant concentrations, could disrupt gonadal differentiation and development in P. nigromaculatus, but we are not sure whether the disrupting effects were associated with masculinization or feminization.
[Mh] Termos MeSH primário: Ranidae/fisiologia
Testículo/efeitos dos fármacos
Triclosan/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Larva/fisiologia
Masculino
Metamorfose Biológica/efeitos dos fármacos
Razão de Masculinidade
Testículo/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 4NM5039Y5X (Triclosan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180227
[St] Status:MEDLINE


  5 / 59119 MEDLINE  
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[PMID]:28471544
[Au] Autor:Sarkar D; Singh SK
[Ad] Endereço:Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, India.
[Ti] Título:Neonatal hypothyroidism affects testicular glucose homeostasis through increased oxidative stress in prepubertal mice: effects on GLUT3, GLUT8 and Cx43.
[So] Source:Andrology;5(4):749-762, 2017 07.
[Is] ISSN:2047-2927
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Thyroid hormones (THs) play an important role in maintaining the link between metabolism and reproduction and the altered THs status is associated with induction of oxidative stress in various organs like brain, heart, liver and testis. Further, reactive oxygen species play a pivotal role in regulation of glucose homeostasis in several organs, and glucose utilization by Leydig cells is essential for testosterone biosynthesis and thus is largely dependent on glucose transporter 8 (GLUT8). Glucose uptake by Sertoli cells is mediated through glucose transporter 3 (GLUT3) under the influence of THs to meet energy requirement of developing germ cells. THs also modulate level of gap junctional protein such as connexin 43 (Cx43), a potential regulator of cell proliferation and apoptosis in the seminiferous epithelium. Although the role of transient neonatal hypothyroidism in adult testis in terms of testosterone production is well documented, the effect of THs deficiency in early developmental period and its role in testicular glucose homeostasis and oxidative stress with reference to Cx43 in immature mice remain unknown. Therefore, the present study was conducted to evaluate the effect of neonatal hypothyroidism on testicular glucose homeostasis and oxidative stress at postnatal days (PND) 21 and 28 in relation to GLUT3, GLUT8 and Cx43. Hypothyroidism induced by 6-propyl-2-thiouracil (PTU) markedly decreased testicular glucose level with considerable reduction in expression level of GLUT3 and GLUT8. Likewise, lactate dehydrogenase (LDH) activity and intratesticular concentration of lactate were also decreased in hypothyroid mice. There was also a rise in germ cell apoptosis with increased expression of caspase-3 in PTU-treated mice. Further, neonatal hypothyroidism affected germ cell proliferation with decreased expression of proliferating cell nuclear antigen (PCNA) and Cx43. In conclusion, our results suggest that neonatal hypothyroidism alters testicular glucose homeostasis via increased oxidative stress in prepubertal mice, thereby affecting germ cell survival and proliferation.
[Mh] Termos MeSH primário: Conexina 43/metabolismo
Proteínas Facilitadoras de Transporte de Glucose/metabolismo
Transportador de Glucose Tipo 3/metabolismo
Glucose/metabolismo
Hipotireoidismo/metabolismo
Estresse Oxidativo
Testículo/metabolismo
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Animais Recém-Nascidos
Antioxidantes/metabolismo
Apoptose
Proliferação Celular
Conexina 43/genética
Modelos Animais de Doenças
Regulação para Baixo
Homeostase
Hipotireoidismo/induzido quimicamente
Hipotireoidismo/genética
Hipotireoidismo/patologia
L-Lactato Desidrogenase/metabolismo
Ácido Láctico/metabolismo
Peroxidação de Lipídeos
Masculino
Camundongos
Antígeno Nuclear de Célula em Proliferação/metabolismo
Propiltiouracila
Testículo/patologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Connexin 43); 0 (GJA1 protein, mouse); 0 (Glucose Transport Proteins, Facilitative); 0 (Glucose Transporter Type 3); 0 (Proliferating Cell Nuclear Antigen); 0 (Slc2a3 protein, mouse); 0 (Slc2a8 protein, mouse); 33X04XA5AT (Lactic Acid); 721M9407IY (Propylthiouracil); EC 1.1.1.27 (L-Lactate Dehydrogenase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1111/andr.12363


  6 / 59119 MEDLINE  
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[PMID]:29385162
[Au] Autor:González CR; Muscarsel Isla ML; Vitullo AD
[Ad] Endereço:Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico- Universidad Maimónides, Buenos Aires, Argentina.
[Ti] Título:The balance between apoptosis and autophagy regulates testis regression and recrudescence in the seasonal-breeding South American plains vizcacha, Lagostomus maximus.
[So] Source:PLoS One;13(1):e0191126, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mammalian testis undergoes deep changes in their architecture and function during photoregression conditions in seasonal breeders. Particularly, the testicular mechanisms that regulate the transition between the active (functional) and inactive (regression) stage vary between species. The aim of the present study was to analyze the incidence of proliferation, apoptosis and autophagy in the testicular seminiferous ephitelium of a seasonal breeder, Lagostomus maximus, during the annual reproductive cycle. We observed that proliferating spermatogonia increased from the active testis until reaching the maximum peak in the activating testis. During the annual reproductive cycle, the quantity of apoptotic-TUNEL positive spermatogonia and meiotic germ cells was constant and this might be regulated by the members of the BCL2 family. Only in the activating testis, apoptosis of germ cells was almost undetectable. The analysis of the autophagic-related proteins BECN1 and LC3 showed their localization in Leydig cells and the germ cells in the active and activating testis. In the inactive testis, BECN1 and LC3 ceased to be immunolocalized within the seminiferous tubules and the mRNA expression of both regulators decreased. Moreover, the expression of BECN1 and LC3 and also the apoptotic index were up regulated in testicular cultures subjected to nutritional stress. These results suggest a possible interaction between apoptosis and autophagy in the active and activating testis (characterized by high metabolic requirement and nutrient), where autophagy could promote survival over cell death. In the inactive testis, the absence of autophagic-related proteins might explain the massive loss of germ cells, suggesting that autophagy plays new and key role in the alterations of the seminiferous epithelium during photoregression.
[Mh] Termos MeSH primário: Apoptose
Autofagia
Cruzamento
Roedores/fisiologia
Estações do Ano
Testículo/citologia
[Mh] Termos MeSH secundário: Animais
Autofagia/genética
Masculino
Estado Nutricional
Reação em Cadeia da Polimerase em Tempo Real
Túbulos Seminíferos/anatomia & histologia
América do Sul
Estresse Fisiológico
Testículo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191126


  7 / 59119 MEDLINE  
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[PMID]:28744861
[Au] Autor:Pan ZJ; Zhu CK; Wang H; Zhou FJ; Qiang XG
[Ad] Endereço:School of Life Science, Huaiyin Normal University, Huaian, 223300, China.
[Ti] Título:Gonadal morphogenesis and sex differentiation in cultured Ussuri catfish Tachysurus ussuriensis.
[So] Source:J Fish Biol;91(3):866-879, 2017 Sep.
[Is] ISSN:1095-8649
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to investigate the optimal developmental time to perform sex reversal in Ussuri catfish Tachysurus ussuriensis, to develop monosex breeding in aquaculture. Systematic observations of gonadal sex differentiation of P. ussiriensis were conducted. The genital ridge formed at 9 days post fertilization (dpf) and germ cells begin to proliferate at 17 dpf. The ovarian cavity began forming on 21 dpf and completed by 25 dpf while presumptive testis remained quiescent. The primary oocytes were at the chromatin nucleolus stage by 30 dpf, the peri-nucleolus stage by 44 dpf and the cortical alveoli stage by 64 dpf. The germinal vesicle migrated towards the animal pole (polarization) at 120 dpf. In presumptive testis, germ cells entered into mitosis and blood vessels appeared in the proximal gonad on 30 dpf. The efferent duct anlage appeared on 36 dpf and formation of seminal lobules with spermatogonia and lobules interstitium occurred at 120 dpf. Therefore, gonadal sex differentiation occurred earlier in females than in males, with the histological differentiation preceding cytologic differentiation in T. ussuriensis. This indicates that undifferentiated gonads directly differentiate into ovary or testis between 17 and 21 dpf and artificial induction of sexual reversal by oral steroid administration must be conducted before 17 dpf.
[Mh] Termos MeSH primário: Peixes-Gato/crescimento & desenvolvimento
Diferenciação Sexual/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Aquicultura/métodos
Proliferação Celular
Feminino
Células Germinativas/citologia
Células Germinativas/efeitos dos fármacos
Masculino
Morfogênese
Ovário/citologia
Ovário/efeitos dos fármacos
Ovário/crescimento & desenvolvimento
Testículo/citologia
Testículo/efeitos dos fármacos
Testículo/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1111/jfb.13388


  8 / 59119 MEDLINE  
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[PMID]:29342173
[Au] Autor:Gautam M; Bhattacharya I; Rai U; Majumdar SS
[Ad] Endereço:Department of Zoology, University of Delhi, Delhi, India.
[Ti] Título:Hormone induced differential transcriptome analysis of Sertoli cells during postnatal maturation of rat testes.
[So] Source:PLoS One;13(1):e0191201, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sertoli cells (Sc) are unique somatic cells of testis that are the target of both FSH and testosterone (T) and regulate spermatogenesis. Although Sc of neonatal rat testes are exposed to high levels of FSH and T, robust differentiation of spermatogonial cells becomes conspicuous only after 11-days of postnatal age. We have demonstrated earlier that a developmental switch in terms of hormonal responsiveness occurs in rat Sc at around 12 days of postnatal age during the rapid transition of spermatogonia A to B. Therefore, such "functional maturation" of Sc, during pubertal development becomes prerequisite for the onset of spermatogenesis. However, a conspicuous difference in robust hormone (both T and FSH) induced gene expression during the different phases of Sc maturation restricts our understanding about molecular events necessary for the spermatogenic onset and maintenance. Here, using microarray technology, we for the first time have compared the differential transcriptional profile of Sc isolated and cultured from immature (5 days old), maturing (12 days old) and mature (60 days old) rat testes. Our data revealed that immature Sc express genes involved in cellular growth, metabolism, chemokines, cell division, MAPK and Wnt pathways, while mature Sc are more specialized expressing genes involved in glucose metabolism, phagocytosis, insulin signaling and cytoskeleton structuring. Taken together, this differential transcriptome data provide an important resource to reveal the molecular network of Sc maturation which is necessary to govern male germ cell differentiation, hence, will improve our current understanding of the etiology of some forms of idiopathic male infertility.
[Mh] Termos MeSH primário: Células de Sertoli/metabolismo
Testículo/crescimento & desenvolvimento
Testículo/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Diferenciação Celular/efeitos dos fármacos
Quimiocinas/genética
Citocinas/genética
Citoesqueleto/genética
Hormônio Foliculoestimulante/metabolismo
Hormônio Foliculoestimulante/farmacologia
Perfilação da Expressão Gênica
Substâncias de Crescimento/genética
Sistema de Sinalização das MAP Quinases/genética
Masculino
Proteínas Monoméricas de Ligação ao GTP/genética
Análise de Sequência com Séries de Oligonucleotídeos
Fagocitose/genética
Ratos
Ratos Wistar
Células de Sertoli/citologia
Células de Sertoli/efeitos dos fármacos
Espermatogênese/efeitos dos fármacos
Espermatogênese/genética
Espermatogênese/fisiologia
Testículo/efeitos dos fármacos
Testosterona/metabolismo
Testosterona/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemokines); 0 (Cytokines); 0 (Growth Substances); 3XMK78S47O (Testosterone); 9002-68-0 (Follicle Stimulating Hormone); EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191201


  9 / 59119 MEDLINE  
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[PMID]:29293683
[Au] Autor:Kawai Y; Oda A; Kanai Y; Goitsuka R
[Ad] Endereço:Division of Development and Aging, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
[Ti] Título:Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.
[So] Source:PLoS One;13(1):e0190702, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PKnox1 (also known as Prep1) belongs to the TALE family of homeodomain transcription factors that are critical for regulating growth and differentiation during embryonic and postnatal development in vertebrates. We demonstrate here that PKnox1 is required for adult spermatogenesis in a germ cell-intrinsic manner. Tamoxifen-mediated PKnox1 loss in the adult testes, as well as its germ cell-specific ablation, causes testis hypotrophy with germ cell apoptosis and, as a consequence, compromised spermatogenesis. In PKnox1-deficient testes, spermatogenesis was arrested at the c-Kit+ spermatogonia stage, with a complete loss of the meiotic spermatocytes, and was accompanied by compromised differentiation of the c-Kit+ spermatogonia. Taken together, these results indicate that PKnox1 is a critical regulator of maintenance and subsequent differentiation of the c-Kit+ stage of spermatogonia in the adult testes.
[Mh] Termos MeSH primário: Proteínas de Homeodomínio/fisiologia
Espermatogênese/fisiologia
Espermatozoides/metabolismo
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Espermatogônias/citologia
Testículo/citologia
Testículo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Homeodomain Proteins); 0 (Pknox1 protein, mouse)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190702


  10 / 59119 MEDLINE  
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[PMID]:29197623
[Au] Autor:Ommati MM; Heidari R; Jamshidzadeh A; Zamiri MJ; Sun Z; Sabouri S; Wang J; Ahmadi F; Javanmard N; Seifi K; Mousapour S; Yeganeh BS
[Ad] Endereço:Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Animal Sciences, School of Agriculture, Shiraz University, Shiraz, Iran; Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi, Agricultural University, Taigu,
[Ti] Título:Dual effects of sulfasalazine on rat sperm characteristics, spermatogenesis, and steroidogenesis in two experimental models.
[So] Source:Toxicol Lett;284:46-55, 2018 Mar 01.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:There are reports of sulfasalazine (Salazosulfapyridine; SASP)-induced reproductive toxicity, but there it is not known whether the SASP molecule or its intestinal metabolites are responsible for this effect. Rats received SASP (150, 300, and 600mg/kg) for 60 consecutive days (in vivo). Additionally, epididymal sperm was isolated and incubated with SASP (10µM-1600µM) (in vitro). Markers of oxidative stress, mitochondrial function, and sperm functionality, along with testis histopathology as well as several steroidogenic genes and proteins, including steroidogenic acute regulatory (StAR) protein, cytochrome P450 side chain cleavage enzyme (P450scc; Cyp11a), 3ß-hydroxysteroid dehydrogenase (3ß-HSD), 17ß-hydroxysteroid dehydrogenase (17ß-HSD) were measured. SASP toxicity was evident as shown by severe testicular histopathological alterations, along with poor sperm parameters and increased markers of oxidative stress. Plasma testosterone level and steroidogenesis-related gene and protein (StAR, 3-beta-HSD, 17-beta-HSD) expressions, as well as mitochondrial membrane potential, were significantly decreased at high doses of SASP (in vivo). Interestingly, in vitro treatment of sperm with SASP not only caused no significant detrimental effect on rat sperm but also increased parameters of sperm functionality and decreased markers of oxidative stress. SASP had paradoxical actions on the rat sperm in these experimental models. The findings might be useful in understanding the mechanism(s) of SASP-induced reproductive toxicity. The present findings have opened a new molecular window into the relationship between disrupted steroidogenesis and mammalian reproduction indices and also are vital regarding clinical administration of SASP and human reproductive health.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/toxicidade
Hormônios Esteroides Gonadais/metabolismo
Espermatogênese/efeitos dos fármacos
Espermatozoides/efeitos dos fármacos
Sulfassalazina/toxicidade
Testículo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Relação Dose-Resposta a Droga
Masculino
Estresse Oxidativo/efeitos dos fármacos
Ratos Sprague-Dawley
Espermatozoides/metabolismo
Espermatozoides/patologia
Testículo/metabolismo
Testículo/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Biomarkers); 0 (Gonadal Steroid Hormones); 3XC8GUZ6CB (Sulfasalazine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171204
[St] Status:MEDLINE



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