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[PMID]:29194680
[Au] Autor:Jenkins S; Ives J; Avery S; Draper H
[Ti] Título:Who gets the gametes? An argument for a points system for fertility patients.
[So] Source:Bioethics;32(1):16-26, 2018 01.
[Is] ISSN:1467-8519
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This paper argues that the convention of allocating donated gametes on a 'first come, first served' basis should be replaced with an allocation system that takes into account more morally relevant criteria than waiting time. This conclusion was developed using an empirical bioethics methodology, which involved a study of the views of 18 staff members from seven U.K. fertility clinics, and 20 academics, policy-makers, representatives of patient groups, and other relevant professionals, on the allocation of donated sperm and eggs. Against these views, we consider some nuanced ways of including criteria in a points allocation system. We argue that such a system is more ethically robust than 'first come, first served', but we acknowledge that our results suggest that a points system will meet with resistance from those working in the field. We conclude that criteria such as a patient's age, potentially damaging substance use, and parental status should be used to allocate points and determine which patients receive treatment and in what order. These and other factors should be applied according to how they bear on considerations like child welfare, patient welfare, and the effectiveness of the proposed treatment.
[Mh] Termos MeSH primário: Temas Bioéticos
Doação Dirigida de Tecido/ética
Células Germinativas
Acesso aos Serviços de Saúde/ética
Infertilidade
Reprodução/ética
[Mh] Termos MeSH secundário: Adulto
Atitude do Pessoal de Saúde
Bioética
Dissidências e Disputas
Feminino
Fertilidade
Seres Humanos
Masculino
Pais
Discriminação Social
Participação dos Interessados
Reino Unido
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:E; IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1111/bioe.12411


  2 / 9475 MEDLINE  
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[PMID]:28744861
[Au] Autor:Pan ZJ; Zhu CK; Wang H; Zhou FJ; Qiang XG
[Ad] Endereço:School of Life Science, Huaiyin Normal University, Huaian, 223300, China.
[Ti] Título:Gonadal morphogenesis and sex differentiation in cultured Ussuri catfish Tachysurus ussuriensis.
[So] Source:J Fish Biol;91(3):866-879, 2017 Sep.
[Is] ISSN:1095-8649
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to investigate the optimal developmental time to perform sex reversal in Ussuri catfish Tachysurus ussuriensis, to develop monosex breeding in aquaculture. Systematic observations of gonadal sex differentiation of P. ussiriensis were conducted. The genital ridge formed at 9 days post fertilization (dpf) and germ cells begin to proliferate at 17 dpf. The ovarian cavity began forming on 21 dpf and completed by 25 dpf while presumptive testis remained quiescent. The primary oocytes were at the chromatin nucleolus stage by 30 dpf, the peri-nucleolus stage by 44 dpf and the cortical alveoli stage by 64 dpf. The germinal vesicle migrated towards the animal pole (polarization) at 120 dpf. In presumptive testis, germ cells entered into mitosis and blood vessels appeared in the proximal gonad on 30 dpf. The efferent duct anlage appeared on 36 dpf and formation of seminal lobules with spermatogonia and lobules interstitium occurred at 120 dpf. Therefore, gonadal sex differentiation occurred earlier in females than in males, with the histological differentiation preceding cytologic differentiation in T. ussuriensis. This indicates that undifferentiated gonads directly differentiate into ovary or testis between 17 and 21 dpf and artificial induction of sexual reversal by oral steroid administration must be conducted before 17 dpf.
[Mh] Termos MeSH primário: Peixes-Gato/crescimento & desenvolvimento
Diferenciação Sexual/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Aquicultura/métodos
Proliferação Celular
Feminino
Células Germinativas/citologia
Células Germinativas/efeitos dos fármacos
Masculino
Morfogênese
Ovário/citologia
Ovário/efeitos dos fármacos
Ovário/crescimento & desenvolvimento
Testículo/citologia
Testículo/efeitos dos fármacos
Testículo/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1111/jfb.13388


  3 / 9475 MEDLINE  
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[PMID]:28450582
[Au] Autor:McConnell MJ; Moran JV; Abyzov A; Akbarian S; Bae T; Cortes-Ciriano I; Erwin JA; Fasching L; Flasch DA; Freed D; Ganz J; Jaffe AE; Kwan KY; Kwon M; Lodato MA; Mills RE; Paquola ACM; Rodin RE; Rosenbluh C; Sestan N; Sherman MA; Shin JH; Song S; Straub RE; Thorpe J; Weinberger DR; Urban AE; Zhou B; Gage FH; Lehner T; Senthil G; Walsh CA; Chess A; Courchesne E; Gleeson JG; Kidd JM; Park PJ; Pevsner J; Vaccarino FM; Brain Somatic Mosaicism Network
[Ti] Título:Intersection of diverse neuronal genomes and neuropsychiatric disease: The Brain Somatic Mosaicism Network.
[So] Source:Science;356(6336), 2017 Apr 28.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Neuropsychiatric disorders have a complex genetic architecture. Human genetic population-based studies have identified numerous heritable sequence and structural genomic variants associated with susceptibility to neuropsychiatric disease. However, these germline variants do not fully account for disease risk. During brain development, progenitor cells undergo billions of cell divisions to generate the ~80 billion neurons in the brain. The failure to accurately repair DNA damage arising during replication, transcription, and cellular metabolism amid this dramatic cellular expansion can lead to somatic mutations. Somatic mutations that alter subsets of neuronal transcriptomes and proteomes can, in turn, affect cell proliferation and survival and lead to neurodevelopmental disorders. The long life span of individual neurons and the direct relationship between neural circuits and behavior suggest that somatic mutations in small populations of neurons can significantly affect individual neurodevelopment. The Brain Somatic Mosaicism Network has been founded to study somatic mosaicism both in neurotypical human brains and in the context of complex neuropsychiatric disorders.
[Mh] Termos MeSH primário: Encéfalo/anormalidades
Transtornos Mentais/genética
Mosaicismo
Doenças do Sistema Nervoso/genética
Células-Tronco Neurais/fisiologia
Neurônios/fisiologia
[Mh] Termos MeSH secundário: Encéfalo/metabolismo
Divisão Celular/genética
Dano ao DNA
Análise Mutacional de DNA/métodos
Reparo do DNA/genética
Replicação do DNA
Genoma Humano
Células Germinativas/metabolismo
Seres Humanos
Rede Nervosa/crescimento & desenvolvimento
Rede Nervosa/metabolismo
Células-Tronco Neurais/metabolismo
Neurônios/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  4 / 9475 MEDLINE  
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[PMID]:29324788
[Au] Autor:Saeidi S; Shapouri F; de Iongh RU; Casagranda F; Sutherland JM; Western PS; McLaughlin EA; Familari M; Hime GR
[Ad] Endereço:Department of Anatomy and Neuroscience, University of Melbourne, Parkville, Australia.
[Ti] Título:Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis.
[So] Source:PLoS One;13(1):e0190925, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ESRP1 regulates alternative splicing, producing multiple transcripts from its target genes in epithelial tissues. It is upregulated during mesenchymal to epithelial transition associated with reprogramming of fibroblasts to iPS cells and has been linked to pluripotency. Mouse fetal germ cells are the founders of the adult gonadal lineages and we found that Esrp1 mRNA was expressed in both male and female germ cells but not in gonadal somatic cells at various stages of gonadal development (E12.5-E15.5). In the postnatal testis, Esrp1 mRNA was highly expressed in isolated cell preparations enriched for spermatogonia but expressed at lower levels in those enriched for pachytene spermatocytes and round spermatids. Co-labelling experiments with PLZF and c-KIT showed that ESRP1 was localized to nuclei of both Type A and B spermatogonia in a speckled pattern, but was not detected in SOX9+ somatic Sertoli cells. No co-localization with the nuclear speckle marker, SC35, which has been associated with post-transcriptional splicing, was observed, suggesting that ESRP1 may be associated with co-transcriptional splicing or have other functions. RNA interference mediated knockdown of Esrp1 expression in the seminoma-derived Tcam-2 cell line demonstrated that ESRP1 regulates alternative splicing of mRNAs in a non-epithelial cell germ cell tumour cell line.
[Mh] Termos MeSH primário: Células Germinativas/metabolismo
Proteínas de Ligação a RNA/metabolismo
Espermatogênese/fisiologia
Testículo/crescimento & desenvolvimento
Testículo/metabolismo
[Mh] Termos MeSH secundário: Processamento Alternativo
Animais
Linhagem Celular Tumoral
Células Cultivadas
Feminino
Expressão Gênica
Células Germinativas/citologia
Masculino
Camundongos Endogâmicos C57BL
RNA Mensageiro/metabolismo
Testículo/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (ESRP1 protein, mouse); 0 (RNA, Messenger); 0 (RNA-Binding Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190925


  5 / 9475 MEDLINE  
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[PMID]:29320538
[Au] Autor:Patch AM; Nones K; Kazakoff SH; Newell F; Wood S; Leonard C; Holmes O; Xu Q; Addala V; Creaney J; Robinson BW; Fu S; Geng C; Li T; Zhang W; Liang X; Rao J; Wang J; Tian M; Zhao Y; Teng F; Gou H; Yang B; Jiang H; Mu F; Pearson JV; Waddell N
[Ad] Endereço:Department of Genetics and Computational Biology, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
[Ti] Título:Germline and somatic variant identification using BGISEQ-500 and HiSeq X Ten whole genome sequencing.
[So] Source:PLoS One;13(1):e0190264, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Technological innovation and increased affordability have contributed to the widespread adoption of genome sequencing technologies in biomedical research. In particular large cancer research consortia have embraced next generation sequencing, and have used the technology to define the somatic mutation landscape of multiple cancer types. These studies have primarily utilised the Illumina HiSeq platforms. In this study we performed whole genome sequencing of three malignant pleural mesothelioma and matched normal samples using a new platform, the BGISEQ-500, and compared the results obtained with Illumina HiSeq X Ten. Germline and somatic, single nucleotide variants and small insertions or deletions were independently identified from data aligned human genome reference. The BGISEQ-500 and HiSeq X Ten platforms showed high concordance for germline calls with genotypes from SNP arrays (>99%). The germline and somatic single nucleotide variants identified in both sequencing platforms were highly concordant (86% and 72% respectively). These results indicate the potential applicability of the BGISEQ-500 platform for the identification of somatic and germline single nucleotide variants by whole genome sequencing. The BGISEQ-500 datasets described here represent the first publicly-available cancer genome sequencing performed using this platform.
[Mh] Termos MeSH primário: Genoma Humano
Células Germinativas
Sequenciamento de Nucleotídeos em Larga Escala/métodos
[Mh] Termos MeSH secundário: Seres Humanos
Mutação INDEL
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190264


  6 / 9475 MEDLINE  
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[PMID]:28745623
[Au] Autor:Lima AC; Jung M; Rusch J; Usmani A; Lopes AM; Conrad DF
[Ad] Endereço:Department of Genetics, Washington University School of Medicine; Graduate Program in Areas of Basic and Applied Biology (GABBA), Abel Salazar Institute of Biomedical Sciences, University of Porto; Instituto de Investigação e Inovação em Saúde, University of Porto; IPATIMUP - Instituto de Patologia
[Ti] Título:A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry.
[So] Source:J Vis Exp;(125), 2017 Jul 12.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fluorescence-activated cell sorting (FACS) has been one of the methods of choice to isolate enriched populations of mammalian testicular germ cells. Currently, it allows the discrimination of up to 9 murine germ cell populations with high yield and purity. This high-resolution in discrimination and purification is possible due to unique changes in chromatin structure and quantity throughout spermatogenesis. These patterns can be captured by flow cytometry of male germ cells stained with fluorescent DNA-binding dyes such as Hoechst-33342 (Hoechst). Herein is a detailed description of a recently developed protocol to isolate mammalian testicular germ cells. Briefly, single cell suspensions are generated from testicular tissue by mechanical dissociation, double stained with Hoechst and propidium iodide (PI) and processed by flow cytometry. A serial gating strategy, including the selection of live cells (PI negative) with different DNA content (Hoechst intensity), is used during FACS sorting to discriminate up to 5 germ cell types. These include, with corresponding average purities (determined by microscopy evaluation): spermatogonia (66%), primary (71%) and secondary (85%) spermatocytes, and spermatids (90%), further separated into round (93%) and elongating (87%) subpopulations. Execution of the entire workflow is straightforward, allows the isolation of 4 cell types simultaneously with the appropriate FACS machine, and can be performed in less than 2 h. As reduced processing time is crucial to preserve the physiology of ex vivo cells, this method is ideal for downstream high-throughput studies of male germ cell biology. Moreover, a standardized protocol for multispecies purification of mammalian germ cells eliminates methodological sources of variables and allows a single set of reagents to be used for different animal models.
[Mh] Termos MeSH primário: Citometria de Fluxo/métodos
Células Germinativas/metabolismo
Espermatogênese/fisiologia
Testículo/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Germinativas/citologia
Masculino
Camundongos
Espermatogônias/citologia
Testículo/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.3791/55913


  7 / 9475 MEDLINE  
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[PMID]:28455236
[Au] Autor:Bechtsi DP; Waters AP
[Ad] Endereço:Institute of Infection, Immunity and Inflammation, College of Medical Veterinary & Life Sciences, Sir Graham Davies Building, University of Glasgow, 120 University Place, Glasgow, G12 8TA Scotland, UK.
[Ti] Título:Genomics and epigenetics of sexual commitment in Plasmodium.
[So] Source:Int J Parasitol;47(7):425-434, 2017 06.
[Is] ISSN:1879-0135
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Malaria is the disease caused by the apicomplexan parasites belonging to the genus Plasmodium. Expanding our arsenal to include transmission-blocking agents in our fight against malaria is becoming increasingly important. Such an implementation requires detailed understanding of the biology of the Plasmodium life cycle stages that are transmissible. Plasmodium gametocytes are the only parasite stage that can be transmitted to the mosquito vector and are the product of sexual development in a small percentage of parasites that continually proliferate in host blood. The critical decision made by asexual erythrocytic stages to cease further proliferation and differentiate into gametocytes, as well as the first steps they take into maturity, have long remained unknown. Recent studies have contributed to a breakthrough in our understanding of this branch point in development. In this review, we will discuss the findings that have allowed us to make this major leap forward in our knowledge of sexual commitment in Plasmodium. We will further propose a model for the mechanism triggering the switch to sexual development, constructed around the proteins currently known to regulate this process. Further insight into sexual commitment and gametocyte development will help identify targets for the development of transmission-blocking malaria therapies.
[Mh] Termos MeSH primário: Epigênese Genética/fisiologia
Genômica
Células Germinativas/fisiologia
Malária/parasitologia
Malária/transmissão
Plasmodium/fisiologia
[Mh] Termos MeSH secundário: Seres Humanos
Reprodução
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180119
[Lr] Data última revisão:
180119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  8 / 9475 MEDLINE  
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[PMID]:29257950
[Au] Autor:Gross-Thebing T; Yigit S; Pfeiffer J; Reichman-Fried M; Bandemer J; Ruckert C; Rathmer C; Goudarzi M; Stehling M; Tarbashevich K; Seggewiss J; Raz E
[Ad] Endereço:Institute of Cell Biology, ZMBE, Von-Esmarch-Straße 56, 48149 Muenster, Germany.
[Ti] Título:The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation.
[So] Source:Dev Cell;43(6):704-715.e5, 2017 Dec 18.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maintaining cell fate relies on robust mechanisms that prevent the differentiation of specified cells into other cell types. This is especially critical during embryogenesis, when extensive cell proliferation, patterning, and migration events take place. Here we show that vertebrate primordial germ cells (PGCs) are protected from reprogramming into other cell types by the RNA-binding protein Dead end (Dnd). PGCs knocked down for Dnd lose their characteristic morphology and adopt various somatic cell fates. Concomitantly, they gain a gene expression profile reflecting differentiation into cells of different germ layers, in a process that we could direct by expression of specific cell-fate determinants. Importantly, we visualized these events within live zebrafish embryos, which provide temporal information regarding cell reprogramming. Our results shed light on the mechanisms controlling germ cell fate maintenance and are relevant for the formation of teratoma, a tumor class composed of cells from more than one germ layer.
[Mh] Termos MeSH primário: Diferenciação Celular/fisiologia
Proteínas de Ligação a RNA/metabolismo
Proteínas de Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular/genética
Movimento Celular
Técnicas de Reprogramação Celular/métodos
Endoderma/fisiologia
Células Germinativas/metabolismo
Células Germinativas/fisiologia
Hibridização In Situ
Proteínas de Ligação a RNA/genética
Proteínas de Ligação a RNA/fisiologia
Peixe-Zebra/metabolismo
Proteínas de Peixe-Zebra/genética
Proteínas de Peixe-Zebra/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA-Binding Proteins); 0 (Zebrafish Proteins); 0 (dead end protein, zebrafish)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  9 / 9475 MEDLINE  
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[PMID]:29207254
[Au] Autor:Sokac AM
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, BCM 125, Houston, TX 77030, USA. Electronic address: sokac@bcm.edu.
[Ti] Título:Seeing a Coastline Paradox in Membrane Reservoirs.
[So] Source:Dev Cell;43(5):541-542, 2017 Dec 04.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this issue of Developmental Cell, Goudarzi et al. (2017) examine the membrane source that allows bleb-based cell migration in vivo. Their work reminds us of the fractal nature of cell surfaces and highlights how the unfolding of these convoluted surfaces contributes to physiologically relevant cell shape change in intact organisms.
[Mh] Termos MeSH primário: Membrana Celular/metabolismo
Forma Celular/fisiologia
Células Germinativas/citologia
[Mh] Termos MeSH secundário: Animais
Citosol/metabolismo
GTP Fosfo-Hidrolases/metabolismo
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
EC 3.6.1.- (GTP Phosphohydrolases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE


  10 / 9475 MEDLINE  
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[PMID]:29173819
[Au] Autor:Goudarzi M; Tarbashevich K; Mildner K; Begemann I; Garcia J; Paksa A; Reichman-Fried M; Mahabaleshwar H; Blaser H; Hartwig J; Zeuschner D; Galic M; Bagnat M; Betz T; Raz E
[Ad] Endereço:Institute for Cell Biology, ZMBE, Von-Esmarch-Strasse 56, 48149 Münster, Germany.
[Ti] Título:Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations.
[So] Source:Dev Cell;43(5):577-587.e5, 2017 Dec 04.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell migration is essential for morphogenesis, organ formation, and homeostasis, with relevance for clinical conditions. The migration of primordial germ cells (PGCs) is a useful model for studying this process in the context of the developing embryo. Zebrafish PGC migration depends on the formation of cellular protrusions in form of blebs, a type of protrusion found in various cell types. Here we report on the mechanisms allowing the inflation of the membrane during bleb formation. We show that the rapid expansion of the protrusion depends on membrane invaginations that are localized preferentially at the cell front. The formation of these invaginations requires the function of Cdc42, and their unfolding allows bleb inflation and dynamic cell-shape changes performed by migrating cells. Inhibiting the formation and release of the invaginations strongly interfered with bleb formation, cell motility, and the ability of the cells to reach their target.
[Mh] Termos MeSH primário: Membrana Celular/metabolismo
Movimento Celular/fisiologia
Forma Celular/fisiologia
Células Germinativas/citologia
Peixe-Zebra
[Mh] Termos MeSH secundário: Actinas/metabolismo
Animais
Estruturas da Membrana Celular/metabolismo
Extensões da Superfície Celular/metabolismo
Células Germinativas/metabolismo
Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE



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