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  1 / 42710 MEDLINE  
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[PMID]:29447173
[Au] Autor:Diao YF; Lin T; Li X; Oqani RK; Lee JE; Kim SY; Jin DI
[Ad] Endereço:Institute of Special Animal & Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
[Ti] Título:Dynamic changes of SETD2, a histone H3K36 methyltransferase, in porcine oocytes, IVF and SCNT embryos.
[So] Source:PLoS One;13(2):e0191816, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:SETD2 (SET domain containing protein 2) acts as a histone H3 lysine 36 (H3K36)-specific methyltransferase and may play important roles in active gene transcription in human cells. However, its expression and role in porcine oocytes and preimplantation embryos are not well understood. Here, we used immunofluorescence and laser scanning confocal microscopy to examine SETD2 expression in porcine fetal fibroblasts, oocytes, and preimplantation embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA), and somatic cell nuclear transfer (SCNT). In porcine fetal fibroblasts, SETD2 expression was detected in interphase cells, but not in M (mitotic)-phase cells. The SETD2 signal was observed in non-surrounded nucleolus (NSN)-stage oocytes, but not in surrounded nucleolus (SN)-, metaphase I (MI)-, or metaphase II (MII)-stage oocytes. The SETD2 signal was detectable in sperm, and undetectable immediately after fertilization, detectable at the 2-cell stage, and peaked at the 4-cell stage of IVF embryos in which porcine embryonic genome is activated. Similar to the pattern found in IVF embryos, the SETD2 signal was absent from PA embryos at the 1-cell stage, but it was detected at the 2-cell stage and thereafter maintained to the blastocyst stage. Interestingly, unlike the IVF and PA embryos, the SETD2 signal was detected throughout the development of SCNT embryos, including at the 1-cell stage. These data suggest that SETD2 may be functional for embryonic gene transcription in porcine preimplantation embryos. It is further speculated that the aberrant expression of SETD2 at the 1-cell stage of porcine SCNT embryos may be a factor in the low efficiency of cloning in pig.
[Mh] Termos MeSH primário: Fertilização In Vitro
Histona-Lisina N-Metiltransferase/metabolismo
Técnicas de Transferência Nuclear
Oócitos/enzimologia
[Mh] Termos MeSH secundário: Animais
Blastocisto
Células Cultivadas
Oócitos/citologia
Partenogênese
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191816


  2 / 42710 MEDLINE  
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[PMID]:29360833
[Au] Autor:Schwarz KRL; de Castro FC; Schefer L; Botigelli RC; Paschoal DM; Fernandes H; Leal CLV
[Ad] Endereço:Universidade de São Paulo (USP), Faculdade de Zootecnia e Engenharia de Alimentos (FZEA), Departamento de Medicina Veterinária, Pirassununga, São Paulo, Brasil.
[Ti] Título:The role of cGMP as a mediator of lipolysis in bovine oocytes and its effects on embryo development and cryopreservation.
[So] Source:PLoS One;13(1):e0191023, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study aimed to determine the influence of cyclic guanosine 3'5'-monophosphate (cGMP) and cGMP-dependent kinase (PKG) during in vitro maturation (IVM) on lipolysis-related parameters in bovine cumulus-oocyte complexes (COCs), and on embryo development and cryosurvival. COCs were matured with cGMP/PKG modulators and assessed for metaphase II rates (MII), cGMP levels, lipid content in oocytes (OO), transcript abundance for genes involved in lipolysis (ATGL) and lipid droplets (PLIN2) in cumulus cells (CC) and OO, and presence of phosphorylated (active) hormone sensitive lipase (HSLser563) in OO. Embryo development, lipid contents and survival to vitrification were also assessed. Phosphodiesterase 5 inhibition (PDE5; cGMP-hydrolyzing enzyme) with 10-5M sildenafil (SDF) during 24 h IVM increased cGMP in COCs (56.9 vs 9.5 fMol/COC in untreated controls, p<0.05) and did not affect on maturation rate (84.3±6.4% MII). Fetal calf serum (FCS) in IVM medium decreased cGMP in COCs compared to bovine serum albumin (BSA) + SDF (19.6 vs 66.5 fMol/COC, respectively, p<0.05). FCS increased lipid content in OO (40.1 FI, p<0.05) compared to BSA (34.6 FI), while SDF decreased (29.8 and 29.6 FI, with BSA or FCS, respectively p<0.05). PKG inhibitor (KT5823) reversed this effect (38.9 FI, p<0.05). ATGL and PLIN2 transcripts were detected in CC and OO, but were affected by cGMP and PKG only in CC. HSLser563 was detected in OO matured with or without modulators. Reduced lipid content in embryos were observed only when SDF was added during IVM and IVC (27.6 FI) compared to its use in either or none of the culture periods (34.2 FI, p<0.05). Survival to vitrification was unaffected by SDF. In conclusion, cGMP and PKG are involved in lipolysis in OO and possibly in CC and embryos; serum negatively affects this pathway, contributing to lipid accumulation, and cGMP modulation may reduce lipid contents in oocytes and embryos, but without improving embryo cryotolerance.
[Mh] Termos MeSH primário: Criopreservação
GMP Cíclico/fisiologia
Desenvolvimento Embrionário
Lipólise/fisiologia
Oócitos/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Metabolismo dos Lipídeos/genética
Inibidores da Fosfodiesterase 5/farmacologia
Fosforilação
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Phosphodiesterase 5 Inhibitors); H2D2X058MU (Cyclic GMP)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191023


  3 / 42710 MEDLINE  
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[PMID]:28462392
[Au] Autor:Li WC; Zhu XY; Ritson E
[Ad] Endereço:University of St Andrews, St Andrews, Fife KY16 9JP, Scotland.
[Ti] Título:Mechanosensory Stimulation Evokes Acute Concussion-Like Behavior by Activating GIRKs Coupled to Muscarinic Receptors in a Simple Vertebrate.
[So] Source:eNeuro;4(2), 2017 Mar-Apr.
[Is] ISSN:2373-2822
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Most vertebrates show concussion responses when their heads are hit suddenly by heavy objects. Previous studies have focused on the direct physical injuries to the neural tissue caused by the concussive blow. We study a similar behavior in a simple vertebrate, the tadpole. We find that concussion-like behavior can be reliably induced by the mechanosensory stimulation of the head skin without direct physical impacts on the brain. Head skin stimulation activates a cholinergic pathway which then opens G protein-coupled inward-rectifying potassium channels (GIRKs) via postsynaptic M muscarinic receptors to inhibit brainstem neurons critical for the initiation and maintenance of swimming for up to minutes and can explain many features commonly observed immediately after concussion. We propose that some acute symptoms of concussion in vertebrates can be explained by the opening of GIRKs following mechanosensory stimulation to the head.
[Mh] Termos MeSH primário: Acetilcolina/metabolismo
Canais de Potássio Corretores do Fluxo de Internalização Acoplados a Proteínas G/metabolismo
Neurônios/metabolismo
Receptores Muscarínicos/metabolismo
[Mh] Termos MeSH secundário: Animais
Concussão Encefálica/metabolismo
Tronco Encefálico/metabolismo
Seres Humanos
Oócitos/metabolismo
Vertebrados/metabolismo
Xenopus laevis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (G Protein-Coupled Inwardly-Rectifying Potassium Channels); 0 (Receptors, Muscarinic); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  4 / 42710 MEDLINE  
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[PMID]:29256287
[Au] Autor:Suttirojpattana T; Somfai T; Matoba S; Nagai T; Parnpai R; Geshi M
[Ad] Endereço:1 Embryo Technology and Stem Cell Research Center, Suranaree University of Technology , Nakhon Ratchasima , Thailand.
[Ti] Título:Effect of storage tube material and resveratrol during liquid storage of matured bovine oocytes on subsequent development.
[So] Source:Acta Vet Hung;65(4):546-555, 2017 12.
[Is] ISSN:0236-6290
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:This study determined the optimum storage vessel and the effects of resveratrol for the storage of in vitro matured (IVM) bovine oocytes. After IVM, the oocytes were kept in a Hepes-buffered medium at 25 °C for 20 h in different containers including Eppendorf tubes (ET) made of polypropylene (PP) and polystyrene (PS), and tissue culture tubes (TCT) made of PP, PS, and glass. Then oocytes were subjected to IVF and subsequent in vitro embryo development was compared among the groups and to that of a control group without storage. The percentage of blastocyst development in the control group was significantly higher than in the stored groups (P < 0.05). Among oocytes stored in TCT, the percentage of blastocyst development of oocytes stored in glass TCT was significantly higher than that of oocytes stored in PP and PS TCT (P < 0.05); however, it did not differ from that of oocytes stored in ET. The quality of blastocysts did not differ among the control and stored groups. Embryo development was not affected when 0.1, 1 or 10 µM resveratrol was added to the medium during oocyte storage. In conclusion, glass tubes were optimal for oocyte storage and resveratrol did not improve the development of stored oocytes.
[Mh] Termos MeSH primário: Bovinos
Fertilização In Vitro/veterinária
Técnicas de Maturação in Vitro de Oócitos/veterinária
Oócitos/efeitos dos fármacos
Oócitos/fisiologia
Estilbenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/farmacologia
Blastocisto/fisiologia
Preservação de Tecido
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Stilbenes); Q369O8926L (resveratrol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.053


  5 / 42710 MEDLINE  
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[PMID]:29358748
[Au] Autor:Kucherenko MM; Shcherbata HR
[Ad] Endereço:Max Planck Research Group of Gene Expression and Signaling, Max Planck Institute for Biophysical Chemistry, Am Fassberg, 11, Goettingen, 37077, Germany.
[Ti] Título:Stress-dependent miR-980 regulation of Rbfox1/A2bp1 promotes ribonucleoprotein granule formation and cell survival.
[So] Source:Nat Commun;9(1):312, 2018 01 22.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Upon stress, profound post-transcriptional adjustments of gene expression occur in spatially restricted, subcellular, membraneless compartments, or ribonucleoprotein (RNP) granules, which are formed by liquid phase separation of RNA-binding proteins with low complexity sequence domains (LCDs). Here, we show that Rbfox1 is an LCD-containing protein that aggregates into liquid droplets and amyloid-like fibers and promiscuously joins different nuclear and cytoplasmic RNP granules. Using Drosophila oogenesis as an in vivo system for stress response, we demonstrate a mechanism by which Rbfox1 promotes cell survival. The stress-dependent miRNA miR-980 acts to buffer Rbfox1 levels, since it targets only those Rbfox1 transcripts that contain extended 3'UTRs. Reduced miR-980 expression during stress leads to increased Rbfox1 levels, widespread formation of various RNP granules, and increased cell viability. We show that human RBFOX proteins also contain multiple LCDs and form membraneless compartments, suggesting that the RNP granule-linked control of cellular adaptive responses may contribute to a wide range of RBFOX-associated pathologies in humans.
[Mh] Termos MeSH primário: Proteínas de Drosophila/genética
MicroRNAs/genética
Oócitos/metabolismo
Proteínas de Ligação a RNA/genética
Ribonucleoproteínas/genética
Estresse Fisiológico/genética
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Animais
Sobrevivência Celular
Reprogramação Celular
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/genética
Drosophila melanogaster/crescimento & desenvolvimento
Drosophila melanogaster/metabolismo
Feminino
Fibroblastos/citologia
Fibroblastos/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Seres Humanos
MicroRNAs/metabolismo
Mutação
Neurônios/citologia
Neurônios/metabolismo
Oócitos/citologia
Oogênese/genética
Ovário/citologia
Ovário/metabolismo
Cultura Primária de Células
Domínios Proteicos
Proteínas de Ligação a RNA/metabolismo
Ribonucleoproteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (MicroRNAs); 0 (RNA-Binding Proteins); 0 (Rbfox1 protein, Drosophila); 0 (Ribonucleoproteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02757-w


  6 / 42710 MEDLINE  
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[PMID]:29292088
[Au] Autor:Yang LL; Zhao Y; Luo SM; Ma JY; Ge ZJ; Shen W; Yin S
[Ad] Endereço:College of Life Sciences, Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
[Ti] Título:Toxic effects and possible mechanisms of hydrogen sulfide and/or ammonia on porcine oocyte maturation in vitro.
[So] Source:Toxicol Lett;285:20-26, 2018 Mar 15.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Previous studies suggest that hydrogen sulfide (H S) and ammonia (NH ) are two major air pollutants which can cause damage to porcine health. However, the mechanisms underlying toxic effects of these compounds on porcine oocyte maturation are not clear. To clarify the mechanism, we evaluated the oocyte quality by detecting some events during oocytes maturation. In our study, porcine oocytes were cultured with different concentrations of Na S and/or NH Cl in vitro and the rate of the first polar body extrusion decreased significantly. Also, actin filament was seriously disrupted to damage the cytoskeleton which resulted in reduced rate of oocyte maturation. We explored the reactive oxygen species (ROS) generation and found that the ROS level was increased significantly after Na S treatment but not after NH Cl treatment. Moreover, early stage apoptosis rate was significantly increased and autophagy protein LC3 B expression level was higher in oocytes treated with Na S and/or NH Cl, which might be caused by ROS elevation. Additionally, exposure to Na S and/or NH Cl also caused ROS generation and early apoptosis in cumulus cells, which might further affect oocyte maturation in vitro. In summary, our data suggested that exposure to H S and/or NH decreased porcine oocyte maturation in vitro, which might be caused by actin disruption, ROS generation, early apoptosis and autophagy.
[Mh] Termos MeSH primário: Poluentes Atmosféricos/toxicidade
Amônia/toxicidade
Sulfeto de Hidrogênio/toxicidade
Oócitos/efeitos dos fármacos
Oogênese/efeitos dos fármacos
[Mh] Termos MeSH secundário: Actinas/metabolismo
Amônia/administração & dosagem
Animais
Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Células Cultivadas
Feminino
Sulfeto de Hidrogênio/administração & dosagem
Oócitos/metabolismo
Oócitos/patologia
Corpos Polares/efeitos dos fármacos
Corpos Polares/metabolismo
Corpos Polares/patologia
Espécies Reativas de Oxigênio/metabolismo
Sus scrofa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Air Pollutants); 0 (Reactive Oxygen Species); 7664-41-7 (Ammonia); YY9FVM7NSN (Hydrogen Sulfide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE


  7 / 42710 MEDLINE  
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[PMID]:28745404
[Au] Autor:Kadlec SM; Johnson RD; Mount DR; Olker JH; Borkholder BD; Schoff PK
[Ad] Endereço:Integrated Biosciences Graduate Program, University of Minnesota-Duluth, Minnesota, USA.
[Ti] Título:Testicular oocytes in smallmouth bass in northeastern Minnesota in relation to varying levels of human activity.
[So] Source:Environ Toxicol Chem;36(12):3424-3435, 2017 Dec.
[Is] ISSN:1552-8618
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Testicular oocytes (TOs) have been found in black bass (Micropterus spp.) from many locations in North America. The presence of TOs is often assumed to imply exposure to estrogenic endocrine disrupting compounds (EDCs); however, a definitive causal relationship has yet to be established, and TO prevalence is not consistently low in fish from areas lacking evident EDC sources. This might indicate any of a number of situations: 1) unknown or unidentified EDCs or EDC sources, 2) induction of TOs by other stressors, or 3) testicular oocytes occurring spontaneously during normal development. In the present study, we analyzed TO occurrence in smallmouth bass (Micropterus dolomieu) from 8 populations in northeastern Minnesota watersheds with differing degrees of human development and, hence, presumed likelihood of exposure to anthropogenic chemicals. Three watersheds were categorized as moderately developed, based on the presence of municipal wastewater discharges and higher human population density (4-81 per km ), and 5 watersheds were minimally developed, with very low human population density (0-1 per km ) and minimal built environment. Testicular tissues from mature fish were evaluated using a semiquantitative method that estimated TO density, normalized by cross-sectional area. Testicular oocyte prevalence and density among populations from moderately developed watersheds was higher than in populations from minimally developed watersheds. However, TO prevalence was unexpectedly high and variable (7-43%) in some populations from minimally developed watersheds, and only weak evidence was found for a relationship between TO density and watershed development, suggesting alternative or more complex explanations for TO presence in smallmouth bass from this region. Environ Toxicol Chem 2017;36:3424-3435. © 2017 SETAC.
[Mh] Termos MeSH primário: Disruptores Endócrinos/toxicidade
Oócitos/efeitos dos fármacos
Testículo/efeitos dos fármacos
Águas Residuais/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Bass
Atividades Humanas
Seres Humanos
Masculino
Minnesota
América do Norte
Oócitos/patologia
Densidade Demográfica
Rios/química
Testículo/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Endocrine Disruptors); 0 (Waste Water); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180218
[Lr] Data última revisão:
180218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1002/etc.3928


  8 / 42710 MEDLINE  
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[PMID]:29305262
[Au] Autor:Ågren R; Sahlholm K; Nilsson J; Århem P
[Ad] Endereço:Department of Neuroscience, Retzius väg 8, Karolinska Institutet, SE-171 77, Stockholm, Sweden. Electronic address: richard.agren@stud.ki.se.
[Ti] Título:Point mutation of a conserved aspartate, D69, in the muscarinic M receptor does not modify voltage-sensitive agonist potency.
[So] Source:Biochem Biophys Res Commun;496(1):101-104, 2018 01 29.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The muscarinic M receptor (M R) has been shown to display voltage-sensitive agonist binding, based on G protein-activated inward rectifier potassium channel (GIRK) opening and radioligand binding at different membrane voltages. A conserved aspartate in transmembrane segment (TM) II of M R, D69, has been proposed as the voltage sensor. While a recent paper instead presented evidence of tyrosines in TMs III, VI, and VII acting as voltage sensors, these authors were not able to record GIRK channel activation by a D69N mutant M R. In the present study, we succeeded in recording ACh-induced GIRK channel activation by this mutant at -80 and 0 mV. The acetylcholine EC was about 2.5-fold higher at 0 mV, a potency shift very similar to that observed at wild-type M R, indicating that voltage sensitivity persists at the D69N mutant. Thus, our present observations corroborate the notion that D69 is not responsible for voltage sensitivity of the M R.
[Mh] Termos MeSH primário: Acetilcolina/administração & dosagem
Canais de Potássio Corretores do Fluxo de Internalização Acoplados a Proteínas G/metabolismo
Potenciais da Membrana/efeitos dos fármacos
Potenciais da Membrana/fisiologia
Receptor Muscarínico M2/genética
Receptor Muscarínico M2/metabolismo
[Mh] Termos MeSH secundário: Animais
Ácido Aspártico/genética
Células Cultivadas
Sequência Conservada
Relação Dose-Resposta a Droga
Mutagênese Sítio-Dirigida
Oócitos
Mutação Puntual/genética
Receptor Muscarínico M2/efeitos dos fármacos
Relação Estrutura-Atividade
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (G Protein-Coupled Inwardly-Rectifying Potassium Channels); 0 (Receptor, Muscarinic M2); 30KYC7MIAI (Aspartic Acid); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE


  9 / 42710 MEDLINE  
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[PMID]:29221621
[Au] Autor:Chaput L; Grémeau AS; Vorilhon S; Pons H; Chabrot C; Grèze V; Pouly JL; Brugnon F
[Ad] Endereço:CHU de Clermont-Ferrand, service de biologie et médecine de la reproduction, AMP-CECOS, site Estaing, 1, place Lucie-et-Raymond-Aubrac, 63003 Clermont-Ferrand, France. Electronic address: laurechaput08@gmail.com.
[Ti] Título:[Fertility preservation in oncology].
[Ti] Título:Préservation de la fertilité en cancérologie..
[So] Source:Bull Cancer;105(1):99-110, 2018 Jan.
[Is] ISSN:1769-6917
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:Since the improvement of cancer diagnosis and treatment, survival rates of these patients increase. Gonadal damages are frequent consequences of cancer treatments with different evidence of impaired fertility. In this context, fertility preservation should be proposed to patients exposed to potentially gonadotoxic treatments. Different preservation approaches may be proposed depending on patient age, sex, cancer type and type of treatment. The indications of fertility preservation depend on sexual maturity. In young girls, ovarian cortex cryopreservation is the only technique feasible in order to preserve their reproductive potential. Vitrification of oocytes which needs ovarian stimulation or oocytes in vitro maturation is becoming more commonly performed for pubertal women to preserve their fertility. Ovarian cortex freezing could be offered to emergency fertility preservation of adult female cancer patients. In prepubertal boys, testicular tissue cryopreservation is the only line treatment for fertility preservation. For future use, various approaches are being evaluated such as spermatogonial stem cell injection or in vitro maturation. Cryopreservation of spermatozoa is, today, an established and successful technique for male adults. When there are no spermatozoa in ejaculate, sperm can be retrieved after treatment of testicular biopsy. The French bioethics law clearly indicates that fertility preservation should be proposed to patients exposed to potentially gonadotoxic treatment. Today, many approaches are possible. Fertility preservation indications are based on multidisciplinary consultations within platforms for the fertility preservation in order to optimize the patient care.
[Mh] Termos MeSH primário: Criopreservação/métodos
Preservação da Fertilidade/métodos
Neoplasias/terapia
Oócitos
Ovário
Espermatozoides
Testículo
[Mh] Termos MeSH secundário: Adulto
Fatores Etários
Embrião de Mamíferos
Feminino
Seres Humanos
Masculino
Fatores Sexuais
Sobreviventes
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171210
[St] Status:MEDLINE


  10 / 42710 MEDLINE  
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[PMID]:28456960
[Au] Autor:García-López J; Larriba E; Del Mazo J
[Ad] Endereço:Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, Madrid 28040, Spain.
[Ti] Título:Detection and Characterization of Small Noncoding RNAs in Mouse Gametes and Embryos Prior to Zygotic Genome Activation.
[So] Source:Methods Mol Biol;1605:105-120, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Small noncoding RNAs (ncRNAs) are regulatory elements of gene expression in all cell types and tissues. An ever-increasing number of studies have implicated ncRNAs in differentiation and developmental processes. In mammals, as a consequence of fertilization, the content of ncRNAs in the zygote is mostly the result of the maternal material included on oocytes and the potential sperm-borne paternal contributions. The genetic identity program of any individual is the reprogramming of each parental contribution to the zygotic genome activation. In mouse, this activation occurs at 2-cell stage. In this program of early development the small ncRNAs can play important roles. Here, we describe protocols for collection of oocytes, spermatozoa, and zygotes in mouse, followed by RNA purification to analyze the different types of small ncRNA by next-generation sequencing approaches (NGS). Bioinformatics protocols also describe the methodology able to characterize microRNAs (miRNAs) as the most well-known and widespread regulatory small ncRNA. The comparative analysis allows identifying the changes and background previous to zygotic genome activation.
[Mh] Termos MeSH primário: Oócitos/química
Pequeno RNA não Traduzido/análise
Espermatozoides/química
Zigoto/química
[Mh] Termos MeSH secundário: Animais
Feminino
Regulação da Expressão Gênica no Desenvolvimento
Sequenciamento de Nucleotídeos em Larga Escala
Masculino
Camundongos
Análise de Sequência de RNA
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Small Untranslated)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6988-3_7



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