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Pesquisa : A05.360.490.690.680.500 [Categoria DeCS]
Referências encontradas : 165 [refinar]
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[PMID]:29292088
[Au] Autor:Yang LL; Zhao Y; Luo SM; Ma JY; Ge ZJ; Shen W; Yin S
[Ad] Endereço:College of Life Sciences, Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
[Ti] Título:Toxic effects and possible mechanisms of hydrogen sulfide and/or ammonia on porcine oocyte maturation in vitro.
[So] Source:Toxicol Lett;285:20-26, 2018 Mar 15.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Previous studies suggest that hydrogen sulfide (H S) and ammonia (NH ) are two major air pollutants which can cause damage to porcine health. However, the mechanisms underlying toxic effects of these compounds on porcine oocyte maturation are not clear. To clarify the mechanism, we evaluated the oocyte quality by detecting some events during oocytes maturation. In our study, porcine oocytes were cultured with different concentrations of Na S and/or NH Cl in vitro and the rate of the first polar body extrusion decreased significantly. Also, actin filament was seriously disrupted to damage the cytoskeleton which resulted in reduced rate of oocyte maturation. We explored the reactive oxygen species (ROS) generation and found that the ROS level was increased significantly after Na S treatment but not after NH Cl treatment. Moreover, early stage apoptosis rate was significantly increased and autophagy protein LC3 B expression level was higher in oocytes treated with Na S and/or NH Cl, which might be caused by ROS elevation. Additionally, exposure to Na S and/or NH Cl also caused ROS generation and early apoptosis in cumulus cells, which might further affect oocyte maturation in vitro. In summary, our data suggested that exposure to H S and/or NH decreased porcine oocyte maturation in vitro, which might be caused by actin disruption, ROS generation, early apoptosis and autophagy.
[Mh] Termos MeSH primário: Poluentes Atmosféricos/toxicidade
Amônia/toxicidade
Sulfeto de Hidrogênio/toxicidade
Oócitos/efeitos dos fármacos
Oogênese/efeitos dos fármacos
[Mh] Termos MeSH secundário: Actinas/metabolismo
Amônia/administração & dosagem
Animais
Apoptose/efeitos dos fármacos
Autofagia/efeitos dos fármacos
Células Cultivadas
Feminino
Sulfeto de Hidrogênio/administração & dosagem
Oócitos/metabolismo
Oócitos/patologia
Corpos Polares/efeitos dos fármacos
Corpos Polares/metabolismo
Corpos Polares/patologia
Espécies Reativas de Oxigênio/metabolismo
Sus scrofa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Air Pollutants); 0 (Reactive Oxygen Species); 7664-41-7 (Ammonia); YY9FVM7NSN (Hydrogen Sulfide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE


  2 / 165 MEDLINE  
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[PMID]:28125646
[Au] Autor:Camlin NJ; McLaughlin EA; Holt JE
[Ad] Endereço:School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
[Ti] Título:Kif4 Is Essential for Mouse Oocyte Meiosis.
[So] Source:PLoS One;12(1):e0170650, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Progression through the meiotic cell cycle must be strictly regulated in oocytes to generate viable embryos and offspring. During mitosis, the kinesin motor protein Kif4 is indispensable for chromosome condensation and separation, midzone formation and cytokinesis. Additionally, the bioactivity of Kif4 is dependent on phosphorylation via Aurora Kinase B and Cdk1, which regulate Kif4 function throughout mitosis. Here, we examine the role of Kif4 in mammalian oocyte meiosis. Kif4 localized in the cytoplasm throughout meiosis I and II, but was also observed to have a dynamic subcellular distribution, associating with both microtubules and kinetochores at different stages of development. Co-localization and proximity ligation assays revealed that the kinetochore proteins, CENP-C and Ndc80, are potential Kif4 interacting proteins. Functional analysis of Kif4 in oocytes via antisense knock-down demonstrated that this protein was not essential for meiosis I completion. However, Kif4 depleted oocytes displayed enlarged polar bodies and abnormal metaphase II spindles, indicating an essential role for this protein for correct asymmetric cell division in meiosis I. Further investigation of the phosphoregulation of meiotic Kif4 revealed that Aurora Kinase and Cdk activity is critical for Kif4 kinetochore localization and interaction with Ndc80 and CENP-C. Finally, Kif4 protein but not gene expression was found to be upregulated with age, suggesting a role for this protein in the decline of oocyte quality with age.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/genética
Proteínas Cromossômicas não Histona/genética
Cinesina/genética
Meiose/genética
Proteínas Associadas aos Microtúbulos/genética
Oócitos/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Aurora Quinase B/genética
Proteína Quinase CDC2/genética
Regulação da Expressão Gênica no Desenvolvimento/genética
Cinetocoros/metabolismo
Camundongos
Microtúbulos/genética
Mitose/genética
Oócitos/metabolismo
Fosforilação
Corpos Polares
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Chromosomal Proteins, Non-Histone); 0 (Hec1 protein, mouse); 0 (Microtubule-Associated Proteins); 0 (centromere protein C); EC 2.7.11.1 (Aurkb protein, mouse); EC 2.7.11.1 (Aurora Kinase B); EC 2.7.11.22 (CDC2 Protein Kinase); EC 3.6.1.- (Kif4 protein, mouse); EC 3.6.4.4 (Kinesin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170650


  3 / 165 MEDLINE  
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[PMID]:27840020
[Au] Autor:Ma H; O'Neil RC; Marti Gutierrez N; Hariharan M; Zhang ZZ; He Y; Cinnioglu C; Kayali R; Kang E; Lee Y; Hayama T; Koski A; Nery J; Castanon R; Tippner-Hedges R; Ahmed R; Van Dyken C; Li Y; Olson S; Battaglia D; Lee DM; Wu DH; Amato P; Wolf DP; Ecker JR; Mitalipov S
[Ad] Endereço:Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR 97239, USA.
[Ti] Título:Functional Human Oocytes Generated by Transfer of Polar Body Genomes.
[So] Source:Cell Stem Cell;20(1):112-119, 2017 Jan 05.
[Is] ISSN:1875-9777
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Oocyte defects lie at the heart of some forms of infertility and could potentially be addressed therapeutically by alternative routes for oocyte formation. Here, we describe the generation of functional human oocytes following nuclear transfer of first polar body (PB1) genomes from metaphase II (MII) oocytes into enucleated donor MII cytoplasm (PBNT). The reconstructed oocytes supported the formation of de novo meiotic spindles and, after fertilization with sperm, meiosis completion and formation of normal diploid zygotes. While PBNT zygotes developed to blastocysts less frequently (42%) than controls (75%), genome-wide genetic, epigenetic, and transcriptional analyses of PBNT and control ESCs indicated comparable numbers of structural variations and markedly similar DNA methylation and transcriptome profiles. We conclude that rescue of PB1 genetic material via introduction into donor cytoplasm may offer a source of oocytes for infertility treatment or mitochondrial replacement therapy for mtDNA disease.
[Mh] Termos MeSH primário: Genoma Humano
Técnicas de Transferência Nuclear
Oócitos/metabolismo
Corpos Polares/metabolismo
[Mh] Termos MeSH secundário: Adulto
Blastocisto/metabolismo
Metilação de DNA/genética
Desenvolvimento Embrionário/genética
Epigênese Genética
Feminino
Fertilização In Vitro
Perfilação da Expressão Gênica
Instabilidade Genômica
Células-Tronco Embrionárias Humanas/metabolismo
Seres Humanos
Masculino
Metáfase
Ploidias
Análise de Sequência de RNA
Espermatozoides/metabolismo
Fuso Acromático/metabolismo
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161115
[St] Status:MEDLINE


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[PMID]:27826797
[Au] Autor:Huang CJ; Yuan YF; Wu D; Khan FA; Jiao XF; Huo LJ
[Ad] Endereço:College of Animal Science and Technology, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry Education, Huazhong Agricultural University, Wuhan, China.
[Ti] Título:The cohesion stabilizer sororin favors DNA repair and chromosome segregation during mouse oocyte meiosis.
[So] Source:In Vitro Cell Dev Biol Anim;53(3):258-264, 2017 Mar.
[Is] ISSN:1543-706X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Maintenance and timely termination of cohesion on chromosomes ensures accurate chromosome segregation to guard against aneuploidy in mammalian oocytes and subsequent chromosomally abnormal pregnancies. Sororin, a cohesion stabilizer whose relevance in antagonizing the anti-cohesive property of Wings-apart like protein (Wapl), has been characterized in mitosis; however, the role of Sororin remains unclear during mammalian oocyte meiosis. Here, we show that Sororin is required for DNA damage repair and cohesion maintenance on chromosomes, and consequently, for mouse oocyte meiotic program. Sororin is constantly expressed throughout meiosis and accumulates on chromatins at germinal vesicle (GV) stage/G2 phase. It localizes onto centromeres from germinal vesicle breakdown (GVBD) to metaphase II stage. Inactivation of Sororin compromises the GVBD and first polar body extrusion (PBE). Furthermore, Sororin inactivation induces DNA damage indicated by positive γH2AX foci in GV oocytes and precocious chromatin segregation in MII oocytes. Finally, our data indicate that PlK1 and MPF dissociate Sororin from chromosome arms without affecting its centromeric localization. Our results define Sororin as a determinant during mouse oocyte meiotic maturation by favoring DNA damage repair and chromosome separation, and thereby, maintaining the genome stability and generating haploid gametes.
[Mh] Termos MeSH primário: Proteínas Adaptadoras de Transdução de Sinal/genética
Proteínas de Ciclo Celular/genética
Centrômero/genética
Meiose/genética
Oócitos/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transdução de Sinal/biossíntese
Animais
Proteínas de Ciclo Celular/biossíntese
Segregação de Cromossomos/genética
Dano ao DNA/genética
Reparo do DNA/genética
Feminino
Proteínas Ligadas por GPI/genética
Regulação da Expressão Gênica no Desenvolvimento
Histonas/genética
Camundongos
Corpos Polares/citologia
Proteínas Serina-Treonina Quinases/genética
Proteínas/genética
Proteínas Proto-Oncogênicas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (Cdca5 protein, mouse); 0 (Cell Cycle Proteins); 0 (GPI-Linked Proteins); 0 (Histones); 0 (Proteins); 0 (Proto-Oncogene Proteins); 0 (WAPL protein, mouse); 0 (gamma-H2AX protein, mouse); 0 (mesothelin); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (polo-like kinase 1)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171105
[Lr] Data última revisão:
171105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE
[do] DOI:10.1007/s11626-016-0107-0


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[PMID]:27416586
[Au] Autor:Wang Z; Feng C; Ang WT; Tan SYM; Latt WT
[Ti] Título:Autofocusing and Polar Body Detection in Automated Cell Manipulation.
[So] Source:IEEE Trans Biomed Eng;64(5):1099-1105, 2017 May.
[Is] ISSN:1558-2531
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Autofocusing and feature detection are two essential processes for performing automated biological cell manipulation tasks. In this paper, we have introduced a technique capable of focusing on a holding pipette and a mammalian cell under a bright-field microscope automatically, and a technique that can detect and track the presence and orientation of the polar body of an oocyte that is rotated at the tip of a micropipette. Both algorithms were evaluated by using mouse oocytes. Experimental results show that both algorithms achieve very high success rates: 100% and 96%. As robust and accurate image processing methods, they can be widely applied to perform various automated biological cell manipulations.
[Mh] Termos MeSH primário: Separação Celular/métodos
Rastreamento de Células/métodos
Interpretação de Imagem Assistida por Computador/métodos
Micromanipulação/métodos
Corpos Polares/citologia
Robótica/métodos
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Aumento da Imagem/métodos
Camundongos
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160715
[St] Status:MEDLINE
[do] DOI:10.1109/TBME.2016.2590995


  6 / 165 MEDLINE  
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[PMID]:27733212
[Au] Autor:Sugano Y; Yazawa M; Takino S; Niimura S; Yamashiro H
[Ad] Endereço:Laboratory of Animal Reproduction,Graduate School of Science and Technology,Niigata University,2-8050 Ikarashi,Nishiku,Niigata 950-2181,Japan.
[Ti] Título:Combination of spindle and first polar body chromosome images for the enhanced prediction of developmental potency of mouse metaphase II oocytes.
[So] Source:Zygote;24(6):900-908, 2016 Dec.
[Is] ISSN:1469-8730
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to classify spindle and first polar body (PB1) chromosome images in ovulated mouse oocytes over time to predict the developmental competence of metaphase II (MII) oocytes. Oocytes were collected at 12, 15, 20, and 25 h after human chorionic gonadotropin (hCG) injection, and stained for spindle tubulin, chromosomes, and PB1 chromosomes. MII spindle morphology was classified as tapered type or barrel type and PB1 chromosomes were categorized as aggregated, separated, dot, or collapsed. To determine whether differences in spindle and PB1 images in MII oocytes are associated with fertilization success, we performed in vitro fertilization (IVF) at various times after hCG injection. Barrel-type spindles and aggregate-type PB1 were dominant at 12 h after hCG injection. Oocyte spindles collected 1 h after injection were tapered, and PB1 chromosomes were separated. At 20 and 25 h after treatment, spindle and PB1 images were classified as collapsed. The rate of development to 2-cell embryos after IVF did not differ between the 12 h and 15 h treatments; however, it was significantly lower for the 25 h treatment than for other treatments. The rates of development to blastocysts at 12, 15, 20, and 25 h after hCG injection were 61, 46, 42, and 9%, respectively. MII oocytes with barrel-type spindles and aggregate-type PB1 had high rates of fertilization and blastocyst development, and spindle and PB1 characteristics were correlated with the outcomes of IVF and embryo culture. These results suggested that images of spindles combined with those of PB1 chromosomes enable the prediction of oocytic and/or embryonic quality.
[Mh] Termos MeSH primário: Cromossomos
Oócitos/fisiologia
Corpos Polares
Fuso Acromático
[Mh] Termos MeSH secundário: Animais
Blastocisto/fisiologia
Feminino
Fertilização In Vitro
Imunofluorescência/métodos
Masculino
Metáfase
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos DBA
Microscopia de Fluorescência
Oócitos/citologia
Ovulação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161014
[St] Status:MEDLINE


  7 / 165 MEDLINE  
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[PMID]:27693251
[Au] Autor:Wang H; Jo YJ; Sun TY; Namgoong S; Cui XS; Oh JS; Kim NH
[Ad] Endereço:Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea.
[Ti] Título:Inhibition of CDK7 bypasses spindle assembly checkpoint via premature cyclin B degradation during oocyte meiosis.
[So] Source:Biochim Biophys Acta;1863(12):2993-3000, 2016 12.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To ensure accurate chromosome segregation, the spindle assembly checkpoint (SAC) delays anaphase onset by preventing the premature activation of anaphase-promoting complex/cyclosome (APC/C) until all kinetochores are attached to the spindle. Although an escape from mitosis in the presence of unsatisfied SAC has been shown in several cancer cells, it has not been reported in oocyte meiosis. Here, we show that CDK7 activity is required to prevent a bypass of SAC during meiosis I in mouse oocytes. Inhibition of CDK7 using THZ1 accelerated the first meiosis, leading to chromosome misalignment, lag of chromosomes during chromosome segregation, and a high incidence of aneuploidy. Notably, this acceleration occurred in the presence of SAC proteins including Mad2 and Bub3 at the kinetochores. However, inhibition of APC/C-mediated cyclin B degradation blocked the THZ1-induced premature polar body extrusion. Moreover, chromosomal defects mediated by THZ1 were rescued when anaphase onset was delayed. Collectively, our results show that CDK7 activity is required to prevent premature anaphase onset by suppressing the bypass of SAC, thus ensuring chromosome alignment and proper segregation. These findings reveal new roles of CDK7 in the regulation of meiosis in mammalian oocytes.
[Mh] Termos MeSH primário: Segregação de Cromossomos/efeitos dos fármacos
Ciclina B/genética
Quinases Ciclina-Dependentes/genética
Meiose/efeitos dos fármacos
Oócitos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Aneuploidia
Animais
Proteínas de Ciclo Celular/genética
Proteínas de Ciclo Celular/metabolismo
Proteínas Cromossômicas não Histona/genética
Proteínas Cromossômicas não Histona/metabolismo
Ciclina B/metabolismo
Quinases Ciclina-Dependentes/antagonistas & inibidores
Quinases Ciclina-Dependentes/metabolismo
Feminino
Regulação da Expressão Gênica
Cinetocoros/metabolismo
Cinetocoros/ultraestrutura
Pontos de Checagem da Fase M do Ciclo Celular/genética
Proteínas Mad2/genética
Proteínas Mad2/metabolismo
Meiose/genética
Camundongos
Camundongos Endogâmicos ICR
Oócitos/citologia
Oócitos/metabolismo
Fenilenodiaminas/farmacologia
Corpos Polares/metabolismo
Corpos Polares/ultraestrutura
Proteínas de Ligação a Poli-ADP-Ribose
Cultura Primária de Células
Proteólise/efeitos dos fármacos
Pirimidinas/farmacologia
Transdução de Sinais
Fuso Acromático/metabolismo
Fuso Acromático/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bub3 protein, mouse); 0 (Cell Cycle Proteins); 0 (Chromosomal Proteins, Non-Histone); 0 (Cyclin B); 0 (Mad2 Proteins); 0 (Mad2l1 protein, mouse); 0 (Phenylenediamines); 0 (Poly-ADP-Ribose Binding Proteins); 0 (Pyrimidines); 0 (THZ1 compound); EC 2.7.11.22 (Cyclin-Dependent Kinases); EC 2.7.11.22 (cyclin-dependent kinase 7, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE


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[PMID]:27650260
[Au] Autor:Mesbah F; Kafi M; Nili H
[Ad] Endereço:Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
[Ti] Título:Cumulus cell expansion and first polar body extrusion during in vitro oocyte maturation in relation to morphological and morphometric characteristics of the dromedary camel ovary.
[So] Source:Reprod Domest Anim;51(6):916-923, 2016 Dec.
[Is] ISSN:1439-0531
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The morphological and morphometric characteristics of the ovary are fundamental properties for in vitro oocyte maturation. Nuclear maturation, including first polar body (1PB) extrusion, cytoplasmic maturation and cumulus cell (CC) expansion are the criteria for in vitro maturation (IVM) of oocyte. This study was designed to determine the effect of morphological and morphometric features of the ovary on CC expansion and 1PB extrusion during IVM of oocyte in the adult female dromedary camel. The weight, volume and three dimensions of ovaries from slaughtered dromedary camels and oocytes inside zona diameter and zona pellucida thickness were measured. The follicles were classified in regard to the size and oocytes according to their ooplasm appearance and CC compactness. Aspirated cumulus oocyte complexes (COCs) were incubated for 48 hr (with a 6-hr interval) in Hams-F10, and CC expansion and 1PB extrusion were assessed. Significant differences were seen in the shape, weight, volume and three dimensions of the ovaries between ≤4-year-old and >4-year-old dromedary camel (p < .5). Approximately, 95.82% of follicles were 2-4 mm in diameter. The mean (±SD) of inside zona diameter of the oocyte and zona pellucida thickness was 132.22 ± 13.8 and 14.64 ± 2.24 µm, respectively, in >4-year-old dromedary camel. The CC expansion and 1PB extrusion were seen in 86% and 21.88% of COCs, respectively. Age and sexual conditions of dromedary camel influence the morphological and morphometric characteristics of the ovary. Most COCs retrieved from 2-6 mm follicles are cultivable. The most slaughterhouse-derived COCs retrieved from 2-6 mm follicles of non-pregnant dromedary camels are excellent and good and yielding a most favourable diameter to achieve the developmental competence for IVM in an optimal time of 24-30 hr; the optimal time for CC expansion is 24-30 hr in this species. However, the CC expansion is a prerequisite process, but not sufficient for IVM.
[Mh] Termos MeSH primário: Células do Cúmulo/fisiologia
Técnicas de Maturação in Vitro de Oócitos/veterinária
Oócitos/fisiologia
Corpos Polares/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160922
[St] Status:MEDLINE
[do] DOI:10.1111/rda.12758


  9 / 165 MEDLINE  
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[PMID]:27582389
[Au] Autor:Li R; Leblanc J; He K; Liu XJ
[Ad] Endereço:Ottawa Hospital Research Institute, Ottawa Hospital-General Campus, Ottawa, ON K1H 8L6, Canada.
[Ti] Título:Spindle function in Xenopus oocytes involves possible nanodomain calcium signaling.
[So] Source:Mol Biol Cell;27(21):3273-3283, 2016 Nov 01.
[Is] ISSN:1939-4586
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intracellular calcium transients are a universal phenomenon at fertilization and are required for egg activation, but the exact role of Ca in second-polar-body emission remains unknown. On the other hand, similar calcium transients have not been demonstrated during oocyte maturation, and yet, manipulating intracellular calcium levels interferes with first-polar-body emission in mice and frogs. To determine the precise role of calcium signaling in polar body formation, we used live-cell imaging coupled with temporally precise intracellular calcium buffering. We found that BAPTA-based calcium chelators cause immediate depolymerization of spindle microtubules in meiosis I and meiosis II. Surprisingly, EGTA at similar or higher intracellular concentrations had no effect on spindle function or polar body emission. Using two calcium probes containing permutated GFP and the calcium sensor calmodulin (Lck-GCaMP3 and GCaMP3), we demonstrated enrichment of the probes at the spindle but failed to detect calcium increase during oocyte maturation at the spindle or elsewhere. Finally, endogenous calmodulin was found to colocalize with spindle microtubules throughout all stages of meiosis. Our results-most important, the different sensitivities of the spindle to BAPTA and EGTA-suggest that meiotic spindle function in frog oocytes requires highly localized, or nanodomain, calcium signaling.
[Mh] Termos MeSH primário: Sinalização do Cálcio/fisiologia
Fuso Acromático/metabolismo
Fuso Acromático/fisiologia
[Mh] Termos MeSH secundário: Animais
Cálcio/metabolismo
Calmodulina/metabolismo
Microambiente Celular/fisiologia
Feminino
Fertilização/fisiologia
Meiose/fisiologia
Microtúbulos/fisiologia
Oócitos/metabolismo
Oócitos/fisiologia
Oogênese/fisiologia
Corpos Polares
Xenopus/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calmodulin); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160902
[St] Status:MEDLINE


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[PMID]:27565256
[Au] Autor:Walls ML; Hart R; Keelan JA; Ryan JP
[Ad] Endereço:Fertility Specialists of Western Australia, Bethesda Hospital, Perth, Western Australia, Australia; School of Women's and Infant's Health, University of Western Australia, King Edward Memorial Hospital, Perth, Western Australia, Australia. Electronic address: melanie@fertilitywa.com.au.
[Ti] Título:Structural and morphologic differences in human oocytes after in vitro maturation compared with standard in vitro fertilization.
[So] Source:Fertil Steril;106(6):1392-1398.e5, 2016 Nov.
[Is] ISSN:1556-5653
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To study whether the size and texture of oocytes/zygotes differ between in vitro maturation (IVM) and traditional IVF and to determine whether these affect the rate of fertilization and blastocyst development. DESIGN: Prospective case-control study. SETTING: Fertility clinic. PATIENT(S): The study involved 83 participants/cycles of IVF with intracytoplasmic sperm injection (ICSI) or IVM treatment. INTERVENTION(S): Participants were allocated to the following groups: patients with and without polycystic ovary syndrome (PCOS) undergoing ICSI (PCOS-ICSI and Control-ICSI), and patients with PCOS undergoing IVM (PCOS-IVM). All oocytes were cultured in an Embryoscope incubator. MAIN OUTCOME MEASURE(S): Oocyte/zygote sizes were recorded and texture parameters of the ooplasm were analyzed using ImageJ and maZda software. Measurements were recorded at five developmental stages: sperm injection, second polar body extrusion, the first pronuclei appearance, pronuclei disappearance, and immediately before cytokinesis. RESULT(S): Normally fertilized PCOS-IVM oocytes were significantly larger at the sperm injection and second polar body extrusion stages, compared with both the PCOS-ICSI and Control-ICSI groups. The PCOS-IVM oocytes were significantly larger at the pronuclei disappearance stage compared with the Control-ICSI group. Oocyte texture parameters were significantly different from both other treatment groups in the early developmental stages, although these were predominantly seen when compared with the Control-ICSI group. There were no significant differences in size or texture by the final stage of immediately before cytokinesis between any of the treatment groups. CONCLUSION(S): This study suggests that oocyte size and texture differ in the early stages of the first cell cycle.
[Mh] Termos MeSH primário: Blastocisto/fisiologia
Técnicas de Maturação in Vitro de Oócitos
Infertilidade Feminina/terapia
Oócitos/fisiologia
Síndrome do Ovário Policístico/terapia
Injeções de Esperma Intracitoplásmicas
Zigoto/fisiologia
[Mh] Termos MeSH secundário: Estudos de Casos e Controles
Citocinese
Técnicas de Cultura Embrionária
Desenvolvimento Embrionário
Feminino
Fertilidade
Fetoscopia
Seres Humanos
Interpretação de Imagem Assistida por Computador
Infertilidade Feminina/diagnóstico
Infertilidade Feminina/etiologia
Infertilidade Feminina/fisiopatologia
Meiose
Recuperação de Oócitos
Indução da Ovulação
Corpos Polares/fisiologia
Síndrome do Ovário Policístico/complicações
Síndrome do Ovário Policístico/diagnóstico
Síndrome do Ovário Policístico/fisiopatologia
Estudos Prospectivos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE



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