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Pesquisa : A05.360.490.890.820.100 [Categoria DeCS]
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[PMID]:28409819
[Au] Autor:Ghosh P; Bhoumik A; Saha S; Mukherjee S; Azmi S; Ghosh JK; Dungdung SR
[Ad] Endereço:Sperm Biology Laboratory, Cell Biology and Physiology Division, CSIR-Indian Institute of Chemical Biology, Kolkata, West Bengal, India.
[Ti] Título:Spermicidal efficacy of VRP, a synthetic cationic antimicrobial peptide, inducing apoptosis and membrane disruption.
[So] Source:J Cell Physiol;233(2):1041-1050, 2018 Feb.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Presently available contraceptives are mostly hormonal or detergent in nature with numerous side effects like irritation, lesion, inflammation in vagina, alteration of body homeostasis, etc. Antimicrobial peptides with spermicidal activity but without adverse effects may be suitable alternatives. In the present study, spermicidal activity of a cationic antimicrobial peptide VRP on human spermatozoa has been elucidated. Progressive forward motility of human spermatozoa was instantly stopped after 100 µM VRP treatment and at 350 µM, all kinds of sperm motility ceased within 20 s as assessed by the Sander-Cramer assay. The spermicidal effect was confirmed by eosin-nigrosin assay and HOS test. VRP treatment (100 µM) in human spermatozoa induced both the intrinsic and extrinsic pathways of apoptosis. TUNEL assay showed VRP treatment significantly disrupted the DNA integrity and changed the mitochondrial membrane permeability as evident from MPTP assay. AFM and SEM results depicted ultra structural changes including disruption of the acrosomal cap and plasma membrane of the head and midpiece region after treatment with 350 µM VRP. MTT assay showed after treatments with 100 and 350 µM of VRP for 24 hr, a substantial amount of Lactobacillus acidophilus (about 90% and 75%, respectively) remained viable. Hence, VRP being a small synthetic peptide with antimicrobial and spermicidal activity but tolerable to normal vaginal microflora, may be a suitable target for elucidating its contraceptive potentiality.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/farmacologia
Apoptose/efeitos dos fármacos
Membrana Celular/efeitos dos fármacos
Peptídeos/farmacologia
Espermicidas/farmacologia
Espermatozoides/efeitos dos fármacos
[Mh] Termos MeSH secundário: Acrossomo/efeitos dos fármacos
Acrossomo/metabolismo
Acrossomo/ultraestrutura
Membrana Celular/metabolismo
Membrana Celular/ultraestrutura
Relação Dose-Resposta a Droga
Seres Humanos
Lactobacillus/efeitos dos fármacos
Masculino
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Viabilidade Microbiana/efeitos dos fármacos
Proteínas de Transporte da Membrana Mitocondrial/efeitos dos fármacos
Proteínas de Transporte da Membrana Mitocondrial/metabolismo
Membranas Mitocondriais/efeitos dos fármacos
Membranas Mitocondriais/metabolismo
Membranas Mitocondriais/ultraestrutura
Permeabilidade
Peça Intermédia do Espermatozoide/efeitos dos fármacos
Peça Intermédia do Espermatozoide/metabolismo
Peça Intermédia do Espermatozoide/ultraestrutura
Motilidade Espermática/efeitos dos fármacos
Espermatozoides/metabolismo
Espermatozoides/ultraestrutura
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (Mitochondrial Membrane Transport Proteins); 0 (Peptides); 0 (Spermatocidal Agents); 0 (mitochondrial permeability transition pore); 25609-85-2 (polyvaline)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.25958


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[PMID]:28859152
[Au] Autor:Khatun A; Rahman MS; Ryu DY; Kwon WS; Pang MG
[Ad] Endereço:Department of Animal Science and Technology, Chung-Ang University, Anseong, Republic of Korea.
[Ti] Título:Elevated aminopeptidase N affects sperm motility and early embryo development.
[So] Source:PLoS One;12(8):e0184294, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aminopeptidase N (APN) is a naturally occurring ectopeptidase present in mammalian semen. Previous studies have demonstrated that APN adversely affects male fertility through the alteration of sperm motility. This enzyme constitutes 0.5 to 1% of the seminal plasma proteins, which can be transferred from the prostasomes to sperms by a fusion process. In the present study, we investigated the molecular mechanism of action of APN and its role in regulating sperm functions and male fertility. In this in vitro study, epididymal mouse spermatozoa were incubated in a capacitating media (pH 7) containing 20 ng/mL of recombinant mouse APN for 90 min. Our results demonstrated that the supplementation of recombinant APN in sperm culture medium significantly increased APN activity, and subsequently altered motility, hyperactivated motility, rapid and medium swimming speeds, viability, and the acrosome reaction of mouse spermatozoa. These effects were potentially caused by increased toxicity in the spermatozoa. Further, altered APN activity in sperm culture medium affected early embryonic development. Interestingly, the effect of elevated APN activity in sperm culture medium was independent of protein tyrosine phosphorylation and protein kinase A activity. On the basis of these results, we concluded that APN plays a significant role in the regulation of several sperm functions and early embryonic development. In addition, increased APN activity could potentially lead to several adverse consequences related to male fertility.
[Mh] Termos MeSH primário: Antígenos CD13/genética
Desenvolvimento Embrionário/genética
Espermatozoides/enzimologia
[Mh] Termos MeSH secundário: Acrossomo/enzimologia
Animais
Antígenos CD13/química
Antígenos CD13/metabolismo
Infertilidade Masculina/enzimologia
Infertilidade Masculina/genética
Masculino
Camundongos
Sêmen/enzimologia
Motilidade Espermática/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.11.2 (CD13 Antigens)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184294


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[PMID]:28850628
[Au] Autor:Lu Y; Aitken RJ; Lin M
[Ad] Endereço:Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Faculty of Science, University of Newcastle, Callaghan, New South Wales, Australia.
[Ti] Título:Ultrastructural investigation and in vitro recapitulation of spermatid differentiation in a potential bio-indicator species - The marine invertebrate Galeolaria gemineoa (Polychaeta: Serpulidae).
[So] Source:PLoS One;12(8):e0183986, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Galeolaria gemineoa is a sessile broadcast-spawning marine invertebrate, whose spermatozoa have been regarded as a sensitive indicator for water quality monitoring. In this study, 10 steps of spermiogenesis have been identified at the ultrastructural level and this differentiation process has been recapitulated in vitro up to the point of spermiogenesis (step 7-9 spermatids). On completion of the second meiosis, newly formed spermatids were detached from the seminiferous epithelium and released to the lumen of each germinal chamber. These spermatids were present in pairs and interconnected by a cytoplasmic bridge throughout the entire spermiogenic process. On the basis of morphological events such as formation of the acrosome, elongation of the flagellum, and condensation of the nucleus, spermiogenesis has been temporally divided into Golgi phase, acrosomal phase and maturation phase. During the Golgi phase, proacrosomal vesicles appeared at the posterior pole of the spermatids and gradually fused into a proacrosomal vacuole. Simultaneously, the distal centriole docked onto the plasma membrane and gave rise to a formative flagellum. The acrosomal phase was characterised by differentiation of the acrosome, condensation of the chromatin and formation of a mitochondrial sheath surrounding the initial portion of the flagellum. During the maturation phase, the fully differentiated acrosome migrated to the anterior pole and excess cytoplasm was extruded from the spermatids in the form of residual bodies. In addition, we successfully induced step 1-3 spermatids to differentiate into the step 7-9 spermatids in both male germinal fluid and 10% foetal bovine serum in RPMI 1640 medium, but failed to replicate this process in female or boiled male germinal fluids. This finding supports our concept that spermatid differentiation in this species is dependent on intrinsic developmental programming and does not require input from accompanying nurse cells.
[Mh] Termos MeSH primário: Membrana Celular/ultraestrutura
Poliquetos/ultraestrutura
Espermátides/ultraestrutura
Espermatogênese/fisiologia
[Mh] Termos MeSH secundário: Acrossomo/ultraestrutura
Animais
Núcleo Celular/ultraestrutura
Citoplasma/ultraestrutura
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183986


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[PMID]:28700739
[Au] Autor:Zhu Z; Ren Z; Fan X; Pan Y; Lv S; Pan C; Lei A; Zeng W
[Ad] Endereço:College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
[Ti] Título:Cysteine protects rabbit spermatozoa against reactive oxygen species-induced damages.
[So] Source:PLoS One;12(7):e0181110, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The process of cryopreservation results in over-production of reactive oxygen species, which is extremely detrimental to spermatozoa. The aim of this study was to investigate whether addition of cysteine to freezing extender would facilitate the cryosurvival of rabbit spermatozoa, and if so, how cysteine protects spermatozoa from cryodamages. Freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with different concentrations of cysteine. The motility, intact acrosomes, membrane integrity, mitochondrial potentials, 8-hydroxyguanosine level and sperm-zona pellucida binding capacity were examined. Furthermore, glutathione peroxidase (GPx) activity, glutathione content (GSH), and level of reactive oxygen species (ROS) and hydrogen peroxide of spermatozoa were analyzed. The values of motility, intact acrosomes, membrane integrity, mitochondrial potentials and sperm-zona pellucida binding capacity of the frozen-thawed spermatozoa in the treatment of cysteine were significantly higher than those of the control. Addition of cysteine to extenders improved the GPx activity and GSH content of spermatozoa, while lowered the ROS, DNA oxidative alterations and lipid peroxidation level, which makes spermatozoa avoid ROS to attack DNA, the plasma membrane and mitochondria. In conclusion, cysteine protects spermatozoa against ROS-induced damages during cryopreservation and post-thaw incubation. Addition of cysteine is recommended to facilitate the improvement of semen preservation for the rabbit breeding industry.
[Mh] Termos MeSH primário: Cisteína/farmacologia
Espécies Reativas de Oxigênio/metabolismo
Espermatozoides/efeitos dos fármacos
[Mh] Termos MeSH secundário: Acrossomo/efeitos dos fármacos
Acrossomo/metabolismo
Animais
Antioxidantes/metabolismo
Criopreservação
Glutationa/metabolismo
Glutationa Peroxidase/metabolismo
Masculino
Coelhos
Preservação do Sêmen
Motilidade Espermática/efeitos dos fármacos
Espermatozoides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Reactive Oxygen Species); EC 1.11.1.9 (Glutathione Peroxidase); GAN16C9B8O (Glutathione); K848JZ4886 (Cysteine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181110


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[PMID]:28627439
[Au] Autor:Xu YR; Fan YS; Yang WX
[Ad] Endereço:The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
[Ti] Título:Mitochondrial prohibitin and its ubiquitination during spermatogenesis of the swimming crab Charybdis japonica.
[So] Source:Gene;627:137-148, 2017 Sep 05.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:It has been proposed that prohibitin (PHB) involved in multiple cellular functions, including cell proliferation, differentiation, apoptosis, senescence and carcinogenesis. Various cellular compartment location of PHB demonstrates its diverse roles. Based on the full-length sequence of PHB gene, we analyzed the deduced amino acid sequence and the predicted protein structure of this gene in the swimming crab Charybdis japonica. It shows that the structure and function of PHB are conservative. The expression level of PHB mRNA and protein in different tissues were analyzed by sqRT-PCR and western blot respectively, which showed its high expression in testis. We then traced PHB protein by immunofluorescence, and we found its diverse distribution in cytoplasm and mitochondria at different stages. We propose that PHB may participate actively in spermatogenic cell anti-apoptosis, cell nucleus distortion as well as acrosome morphogenesis during the spermatogenesis in Charybdis japonica. Furthermore, PHB was found to be ubiquitinated at different levels. Its signal was weak in spermatocytes and Stage 1 spermatids, stronger in stage 2-4 spermatids, and lowest in mature sperm. Our data shows that PHB may mediate the paternal mitochondrial material degradation by ubiquitination. We conclude that PHB is indispensable in the spermatogenesis of the swimming crab Charybdis japonica through different testis developmental stages.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/metabolismo
Braquiúros/fisiologia
Proteínas Repressoras/metabolismo
[Mh] Termos MeSH secundário: Acrossomo
Animais
Núcleo Celular/metabolismo
Clonagem Molecular
Masculino
Reação em Cadeia da Polimerase
Espermatogênese
Espermatozoides/citologia
Espermatozoides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (Repressor Proteins); 0 (prohibitin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


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[PMID]:28509550
[Au] Autor:Huang H; Rodolis MT; Bhatia SR; Sampson NS
[Ad] Endereço:Department of Chemistry, Stony Brook University , Stony Brook, New York 11794-3400, United States.
[Ti] Título:Sugars Require Rigid Multivalent Displays for Activation of Mouse Sperm Acrosomal Exocytosis.
[So] Source:Biochemistry;56(22):2779-2786, 2017 Jun 06.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As a prerequisite to mammalian fertilization, the sperm acrosomal vesicle fuses with the plasma membrane and the acrosome contents are exocytosed. Induction occurs through engagement of the sperm receptors by multiple sugar residues. Multivalent polymers displaying mannose, fucose, or GlcNAc are effective synthetic inducers of mouse sperm acrosomal exocytosis (AE). Each carbohydrate is proposed to have a distinct binding site on the sperm cell surface. To determine the role of the scaffold structure in the efficiency of AE induction, different polymer backbones were employed to display the different activating sugar residues. These glycopolymers were prepared by ruthenium-catalyzed ring-opening metathesis of 5-substituted norbornene or cyclooctene. The conformations of the glycopolymers were characterized by small-angle X-ray scattering. Polynorbornene displaying mannose, fucose, or GlcNAc forms flexible cylinders in aqueous solution. However, polycyclooctenes displaying any of these same sugars are much more flexible and form random coils. The flexible polycyclooctenes displaying fucose or GlcNAc were less effective inducers of AE than their norbornene counterparts. In contrast, polycyclooctene displaying mannose was the most effective AE inducer and had a more collapsed spherelike structure. Our results suggest that the AE efficacy of fucose, GlcNAc, and mannose polymers relies on a relatively rigid polymer that can stabilize receptor signaling complexes.
[Mh] Termos MeSH primário: Acrossomo
Carboidratos
Exocitose
[Mh] Termos MeSH secundário: Animais
Espectroscopia de Ressonância Magnética Nuclear de Carbono-13
Masculino
Camundongos
Espectroscopia de Prótons por Ressonância Magnética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00166


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[PMID]:28476888
[Au] Autor:Oberheide K; Puchkov D; Jentsch TJ
[Ad] Endereço:From the Leibniz-Forschungsinstitut für Molekulare Pharmakologie, D-13125 Berlin and.
[Ti] Título:Loss of the Na /H exchanger NHE8 causes male infertility in mice by disrupting acrosome formation.
[So] Source:J Biol Chem;292(26):10845-10854, 2017 Jun 30.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mammalian sperm feature a specialized secretory organelle on the anterior part of the sperm nucleus, the acrosome, which is essential for male fertility. It is formed by a fusion of Golgi-derived vesicles. We show here that the predominantly Golgi-resident Na /H exchanger NHE8 localizes to the developing acrosome of spermatids. Similar to wild-type mice, mice generated Golgi-derived vesicles positive for acrosomal markers and attached to nuclei, but these vesicles failed to form large acrosomal granules and the acrosomal cap. Spermatozoa from mice completely lacked acrosomes, were round-headed, exhibited abnormal mitochondrial distribution, and displayed decreased motility, resulting in selective male infertility. Of note, similar features are also found in globozoospermia, one of the causes of male infertility in humans. Germ cell-specific, but not Sertoli cell-specific disruption recapitulated the globozoospermia phenotype, demonstrating that NHE8's role in spermiogenesis is germ cell-intrinsic. Our work has uncovered a crucial role of NHE8 in acrosome biogenesis and suggests that some forms of human globozoospermia might be caused by a loss of function of this Na /H exchanger. It points to as a candidate gene for human globozoospermia and a possible drug target for male contraception.
[Mh] Termos MeSH primário: Acrossomo/metabolismo
Núcleo Celular/metabolismo
Trocadores de Sódio-Hidrogênio/metabolismo
Motilidade Espermática
Espermatogênese
Teratozoospermia/metabolismo
[Mh] Termos MeSH secundário: Acrossomo/patologia
Animais
Núcleo Celular/genética
Núcleo Celular/patologia
Seres Humanos
Masculino
Camundongos
Camundongos Knockout
Células de Sertoli/metabolismo
Células de Sertoli/patologia
Trocadores de Sódio-Hidrogênio/genética
Teratozoospermia/genética
Teratozoospermia/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (SLC9A8 protein, human); 0 (Slc9a8 protein, mouse); 0 (Sodium-Hydrogen Exchangers)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170507
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.784108


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[PMID]:28438518
[Au] Autor:Novais AM; Dias G; Lino-Neto J
[Ad] Endereço:Departamento de Biologia Geral, Universidade Federal de Viçosa, 36570-900, Viçosa, Minas Gerais, Brazil; Instituto Federal de Educação, Ciência e Tecnologia do Mato Grosso, Campus Juína, 78320-000, Juína, Mato Grosso, Brazil.
[Ti] Título:Testicular, spermatogenesis and sperm morphology in Martarega bentoi (Heteroptera: Notonectidae).
[So] Source:Arthropod Struct Dev;46(4):635-643, 2017 Jul.
[Is] ISSN:1873-5495
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The testicular, spermatogenesis and sperm morphology of the backswimmer Martarega bentoi was described using light and transmission electron microscopy. In this species, a pair of testes, two deferent ducts, two different pairs of accessory glands, and an ejaculatory duct form the male reproductive system. Each testis consists of two testicular follicles, which are arranged side by side in snail shape. The follicles are filled with cysts at different stages of spermatogenesis, but in the same cyst the germ cells (up to 64) are in the same stage. At the end of spermatogenesis, the sperm cells are very long, with the flagellum measuring approximately 2500 µm in length, the nucleus only 19 µm, and the acrosome, with two distinct regions, 300 µm. The flagellum is composed of an axoneme, with a 9 + 9 + 2 microtubular pattern, and 2 asymmetric mitochondrial derivatives (MDs). These have the anterior ends inserted into two cavities at the nucleus base, exhibit two paracrystalline inclusions, and have bridges linking them to the axoneme. Few spermatozoa per cyst, asymmetry in size and shape of the MDs, as well as their insertion at the nuclear base are characteristics considered derived, and that differentiate the sperm of M. bentoi from those of the Nepomorpha, Belostomatidae and Nepidae.
[Mh] Termos MeSH primário: Heterópteros/ultraestrutura
Espermatogênese
Testículo/ultraestrutura
[Mh] Termos MeSH secundário: Acrossomo/ultraestrutura
Animais
Heterópteros/anatomia & histologia
Masculino
Microscopia Eletrônica de Transmissão
Espermatozoides/ultraestrutura
Testículo/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE


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[PMID]:28370846
[Au] Autor:Chuawongboon P; Sirisathien S; Pongpeng J; Sakhong D; Nagai T; Vongpralub T
[Ad] Endereço:Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
[Ti] Título:Effects of supplementation of iodixanol to semen extender on quality and fertilization ability of frozen-thawed Thai native bull sperm.
[So] Source:Anim Sci J;88(9):1311-1320, 2017 Sep.
[Is] ISSN:1740-0929
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:This study investigates the effects of iodixanol supplementation in varied concentrations to Tris egg yolk (TEY) extender on the quality and fertilization ability of frozen-thawed sperm of Thai native bulls. Each ejaculate was divided into four different groups, as follows: sperm were treated with TEY extender (control group) and TEY extender supplemented with three different concentrations of iodixanol (1.25%, 2.50% and 5.00%). Semen straws were frozen in liquid nitrogen vapor. After thawing, sperm motility characteristics, viability, plasma membrane integrity and acrosome integrity were determined. Also, frozen-thawed spermatozoa from all groups were used for in vitro fertilization and artificial insemination (AI) in natural estrus Thai native cows. The results showed that the post-thaw quality of the 2.50% iodixanol group was superior to the other iodixanol groups (P < 0.05). However, iodixanol had no beneficial effect on post-thaw sperm in vitro fertilization ability and pregnancy rate after AI (P > 0.05). It can be concluded that the supplementation of 2.50% iodixanol extender significantly improves the progressive motility, viability, plasma membrane integrity and acrosome integrity of cryopreserved semen from Thai native bulls, but it has no beneficial effect on in vitro fertilization ability and pregnancy rate after AI.
[Mh] Termos MeSH primário: Criopreservação
Fertilização/efeitos dos fármacos
Fertilização/fisiologia
Análise do Sêmen
Preservação do Sêmen
Sêmen/fisiologia
Espermatozoides/fisiologia
Ácidos Tri-Iodobenzoicos/farmacologia
[Mh] Termos MeSH secundário: Acrossomo/fisiologia
Animais
Bovinos
Membrana Celular/fisiologia
Sobrevivência Celular
Feminino
Inseminação Artificial
Masculino
Motilidade Espermática
Tailândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Triiodobenzoic Acids); HW8W27HTXX (iodixanol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1111/asj.12798


  10 / 3888 MEDLINE  
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[PMID]:28242104
[Au] Autor:Aire TA; du Plessis L; Deokar MS; Rennie E; Gupta SK
[Ad] Endereço:Department of Anatomy, Physiology & Pharmacology, School of Veterinary Medicine, St. George's University, True Blue, St. George's, Grenada. Electronic address: taire@sgu.edu.
[Ti] Título:Structural features of the spermatozoon of a passeridan bird, the Carib grackle, Quiscalus lugubris.
[So] Source:Tissue Cell;49(2 Pt B):233-238, 2017 Apr.
[Is] ISSN:1532-3072
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:The spermatozoon of the Carib grackle, Quiscalus lugubris, a member of the family Icteridae, is generally similar in organization to the passerine-type of spermatozoon, in being highly elongated and displaying a helical structure of the acrosome, nucleus and principal piece of the tail. There are subtle variations in acrosomal structural features between this organelle in the grackle and that in some of the very few passerine species of birds in which the spermatozoon has been studied. The proximal centriole is present, and, thus, the Carib grackle is the third passeridan bird in which this organelle, hitherto regarded as absent in passerine birds, has been described in the spermatozoon. The spermatozoon of this bird also possesses a granular helix, which feature has been found variably even in the scanty available reports on passerine spermatozoa. It is advocated that the spermatozoon be studied in many more species of this large clade of birds. This report provides a basis for the study of spermiogenesis in the Carib grackle, with the aim of exposing, inter alia, a number of developmental features and processes of certain organelles that have received attention, recently, in the spermatozoa of passerine birds.
[Mh] Termos MeSH primário: Acrossomo/ultraestrutura
Espermatogênese/genética
Espermatozoides/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Centríolos/ultraestrutura
Masculino
Passeriformes/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE



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