Base de dados : MEDLINE
Pesquisa : A05.360.490.890.880 [Categoria DeCS]
Referências encontradas : 3593 [refinar]
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  1 / 3593 MEDLINE  
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[PMID]:29331479
[Au] Autor:Zhao C; Zhu W; Yin S; Cao Q; Zhang H; Wen X; Zhang G; Xie W; Chen S
[Ad] Endereço:College of Life Sciences, Key Laboratory of Biodiversity and Biotechnology of Jiangsu Province, Nanjing Normal University, Nanjing, Jiangsu 210023, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China.
[Ti] Título:Molecular characterization and expression of Piwil1 and Piwil2 during gonadal development and treatment with HCG and LHRH-A in Odontobutis potamophila.
[So] Source:Gene;647:181-191, 2018 Mar 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Piwi proteins play an important regulatory role in germ cell division during gametogenesis and gonad development. In order to understand the function of Piwi genes in the reproductive process of the dark sleeper, we identified and characterized Piwil1 and Piwil2 from gonad tissue. The tissue distribution demonstrated that Piwils were highly expressed in the gonad of the dark sleeper. During gonad development, higher expression was observed in stage I of both the testes and ovaries than in subsequent stages at mRNA and protein levels. The results of immunohistochemistry demonstrated that Piwils were predominantly distributed in the spermatogonia, spermatocytes, and early oocytes. When treated with the HPG axis hormone (HCG and LHRH-A2), the expression of Piwils was significantly decreased in the testes and ovaries at mRNA and protein levels. All of these results indicated that Piwils play a vital role in gonad development and gametogenesis. Our findings provide valuable evidence to further clarify the underlying modulation mechanism of Piwils in teleosts.
[Mh] Termos MeSH primário: Proteínas Argonauta/genética
Gonadotropina Coriônica/farmacologia
Regulação da Expressão Gênica no Desenvolvimento/genética
Hormônio Liberador de Gonadotropina/farmacologia
Gônadas/efeitos dos fármacos
Perciformes/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Gametogênese/efeitos dos fármacos
Gametogênese/genética
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Masculino
Oócitos/efeitos dos fármacos
Filogenia
RNA Mensageiro/genética
Espermatócitos/efeitos dos fármacos
Espermatogônias/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Argonaute Proteins); 0 (Chorionic Gonadotropin); 0 (RNA, Messenger); 33515-09-2 (Gonadotropin-Releasing Hormone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180115
[St] Status:MEDLINE


  2 / 3593 MEDLINE  
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[PMID]:29324782
[Au] Autor:Shawki HH; Oishi H; Usui T; Kitadate Y; Basha WA; Abdellatif AM; Hasegawa K; Okada R; Mochida K; El-Shemy HA; Muratani M; Ogura A; Yoshida S; Takahashi S
[Ad] Endereço:Department of Anatomy and Embryology, Graduate School of Comprehensive Human Science, University of Tsukuba, Tsukuba, Japan.
[Ti] Título:MAFB is dispensable for the fetal testis morphogenesis and the maintenance of spermatogenesis in adult mice.
[So] Source:PLoS One;13(1):e0190800, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The transcription factor MAFB is an important regulator of the development and differentiation of various organs and tissues. Previous studies have shown that MAFB is expressed in embryonic and adult mouse testes and is expected to act as the downstream target of retinoic acid (RA) to initiate spermatogenesis. However, its exact localization and function remain unclear. Here, we localized MAFB expression in embryonic and adult testes and analyzed its gene function using Mafb-deficient mice. We found that MAFB and c-MAF are the only large MAF transcription factors expressed in testes, while MAFA and NRL are not. MAFB was localized in Leydig and Sertoli cells at embryonic day (E) 18.5 but in Leydig cells, Sertoli cells, and pachytene spermatocytes in adults. Mafb-deficient testes at E18.5 showed fully formed seminiferous tubules with no abnormal structure or differences in testicular somatic cell numbers compared with those of control wild-type mice. Additionally, the expression levels of genes related to development and function of testicular cells were unchanged between genotypes. In adults, the expression of MAFB in Sertoli cells was shown to be stage specific and induced by RA. By generating Mafbfl/fl CAG-CreER™ (Mafb-cKO) mice, in which Cre recombinase was activated upon tamoxifen treatment, we found that the neonatal cKO mice died shortly upon Mafb deletion, but adult cKO mice were alive upon deletion. Adult cKO mice were fertile, and spermatogenesis maintenance was normal, as indicated by histological analysis, hormone levels, and germ cell stage-specific markers. Moreover, there were no differences in the proportion of seminiferous stages between cKO mice and controls. However, RNA-Seq analysis of cKO Sertoli cells revealed that the down-regulated genes were related to immune function and phagocytosis activity but not spermatogenesis. In conclusion, we found that MAFB is dispensable for fetal testis morphogenesis and spermatogenesis maintenance in adult mice, despite the significant gene expression in different cell types, but MAFB might be critical for phagocytosis activity of Sertoli cells.
[Mh] Termos MeSH primário: Fator de Transcrição MafB/metabolismo
Espermatogênese/fisiologia
Testículo/crescimento & desenvolvimento
Testículo/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Feminino
Fertilidade/fisiologia
Células Intersticiais do Testículo/citologia
Células Intersticiais do Testículo/metabolismo
Fator de Transcrição MafB/genética
Masculino
Camundongos Endogâmicos C57BL
Camundongos Knockout
Proteínas Proto-Oncogênicas c-maf/metabolismo
RNA Mensageiro/metabolismo
Células de Sertoli/citologia
Células de Sertoli/metabolismo
Espermatócitos/citologia
Espermatócitos/metabolismo
Testículo/anatomia & histologia
Testosterona/metabolismo
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Maf protein, mouse); 0 (MafB Transcription Factor); 0 (Mafb protein, mouse); 0 (Proto-Oncogene Proteins c-maf); 0 (RNA, Messenger); 3XMK78S47O (Testosterone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190800


  3 / 3593 MEDLINE  
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[PMID]:29372794
[Au] Autor:Safronova LD; Kupriyanova LA
[Ti] Título:[Metaphase and meiotic chromosomes, synaptonemal complexes (SC) of the lizard Zootoca vivipara].
[So] Source:Genetika;52(11):1311-7, 2016 Nov.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Somatic mitotic and meiotic chromosomes at the pachytene and at the metaphase I of the males of the viviparous lizard, Zootoca vivipara (Lichtenstein, 1823), from northwestern Russia, belonging to the Russian form of Z. v. vivipara, are examined. The spreading of synaptonemal complexes (SC) of their chromosomes are obtained and analyzed for the first time. Eighteen SC are observed, including SC of the Z1Z1 (pairs 5 or 6) and the Z2Z2 (pair 13) sex chromosomes. Characteristics of SC are compared with the number and the shape of bivalents and with those of the karyotype structure. In the studied Russian form of Z. v. vivipara, the length ratios of bivalents correlate with that of mitotic chromosomes (2n = 36); however, some specificity in the morphology of SC of the Z1Z1 sex chromosomes is reported in this article.
[Mh] Termos MeSH primário: Lagartos/metabolismo
Metáfase/fisiologia
Espermatócitos/metabolismo
Complexo Sinaptonêmico/metabolismo
[Mh] Termos MeSH secundário: Animais
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


  4 / 3593 MEDLINE  
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[PMID]:29368479
[Au] Autor:Lovinskaya AV; Kolumbayeva SZ; Kolomiets OL; Abilev SK
[Ti] Título:[Genotoxic effects of pesticide fipronil in somatic and generative cells of mice].
[So] Source:Genetika;52(5):561-8, 2016 May.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The pronounced genotoxic effect of fipronil in all used doses (4.75, 9.50, 19.00, and 31.70 mg/kg) at a single exposure in the liver, lungs and spleen was ascertained by the Comet assay. Organ specificity of genotoxic effects of the pesticide was revealed. The liver was the most sensitive to fipronil. Fipronil at a dose of 9.50 mg/kg in a single and repeated exposure (within 10 days) induced aberrations in mouse bone marrow cells with the frequency exceeding the spontaneous mutation rate (p < 0.01 and p < 0.001, respectively). Fipronil also showed genotoxic activity in the germ cells of the experimental animals, causing abnormalities of the structure of synaptonemal complexes in the spermatocytes.
[Mh] Termos MeSH primário: Células da Medula Óssea/metabolismo
Dano ao DNA
Mutação
Praguicidas/toxicidade
Pirazóis/toxicidade
Espermatócitos/metabolismo
Complexo Sinaptonêmico/metabolismo
[Mh] Termos MeSH secundário: Animais
Células da Medula Óssea/patologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Especificidade de Órgãos/efeitos dos fármacos
Espermatócitos/patologia
Complexo Sinaptonêmico/genética
Complexo Sinaptonêmico/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pesticides); 0 (Pyrazoles); QGH063955F (fipronil)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


  5 / 3593 MEDLINE  
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[PMID]:29216642
[Au] Autor:Yang W; Huang J; Xiao B; Liu Y; Zhu Y; Wang F; Sun S
[Ti] Título:Taurine Protects Mouse Spermatocytes from Ionizing Radiation-Induced Damage Through Activation of Nrf2/HO-1 Signaling.
[So] Source:Cell Physiol Biochem;44(4):1629-1639, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: The increasing prevalence of ionizing radiation exposure has inevitably raised public concern over the potential detrimental effects of ionizing radiation on male reproductive system function. The detection of drug candidates to prevent reproductive system from damage caused by ionizing radiation is urgent. We aimed to investigate the protective role of taurine on the injury of mouse spermatocyte-derived cells (GC-2) subjected to ionizing radiation. METHODS: mouse spermatocytes (GC-2 cells) were exposed to ionizing radiation with or without treatment of Taurine. The effect of ionizing radiation and Taurine treatment on GC-2 cells were evaluated by cell viability assay (CCK8), cell cycle and apoptosis. The relative protein abundance change was determined by Western blotting. The siRNA was used to explore whether Nrf2 signaling was involved in the cytoprotection of Taurine. RESULTS: Taurine significantly inhibited the decrease of cell viability, percentage of apoptotic cells and cell cycle arrest induced by ionizing radiation. Western blot analysis showed that taurine significantly limited the ionizing radiation-induced down-regulation of CyclinB1 and CDK1, and suppressed activation of Fas/FasL system pathway. In addition, taurine treatment significantly increased the expression of Nrf2 and HO-1 in GC-2 cells exposed to ionizing radiation, two components in antioxidant pathway. The above cytoprotection of Taurine was blocked by siNrf2. CONCLUSION: Our results demonstrate that taurine has the potential to effectively protect GC-2 cells from ionizing radiation- triggered damage via upregulation of Nrf2/HO-1 signaling.
[Mh] Termos MeSH primário: Heme Oxigenase-1/metabolismo
Fator 2 Relacionado a NF-E2/metabolismo
Substâncias Protetoras/farmacologia
Radiação Ionizante
Transdução de Sinais/efeitos dos fármacos
Taurina/farmacologia
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Apoptose/efeitos da radiação
Proteína Quinase CDC2/metabolismo
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Pontos de Checagem do Ciclo Celular/efeitos da radiação
Linhagem Celular
Ciclina B1/metabolismo
Regulação para Baixo/efeitos dos fármacos
Proteína Ligante Fas/metabolismo
Masculino
Camundongos
Fator 2 Relacionado a NF-E2/antagonistas & inibidores
Fator 2 Relacionado a NF-E2/genética
Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/efeitos da radiação
Interferência de RNA
RNA Interferente Pequeno/metabolismo
Transdução de Sinais/efeitos da radiação
Espermatócitos/citologia
Espermatócitos/efeitos dos fármacos
Espermatócitos/metabolismo
Receptor fas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclin B1); 0 (Fas Ligand Protein); 0 (Fas protein, mouse); 0 (Fasl protein, mouse); 0 (NF-E2-Related Factor 2); 0 (Protective Agents); 0 (RNA, Small Interfering); 0 (fas Receptor); 1EQV5MLY3D (Taurine); EC 1.14.14.18 (Heme Oxygenase-1); EC 2.7.11.22 (CDC2 Protein Kinase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1159/000485762


  6 / 3593 MEDLINE  
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[PMID]:29174386
[Au] Autor:Li T; Lu Z; Luo R; Gao J; Zhao X; Ma Y
[Ad] Endereço:College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; Sheep Breeding Biotechnology Engineering Laboratory of Gansu Province, Minqin 733300, China.
[Ti] Título:Expression and cellular localization of double sex and mab-3 related transcription factor 1 in testes of postnatal Small-Tail Han sheep at different developmental stages.
[So] Source:Gene;642:467-473, 2018 Feb 05.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Double sex and mab-3 related transcription factor 1 (Dmrt1), an evolutionarily conserved gene, is a sex-related gene expressed in male gonads, that is involved in the regulation of sex differentiation, testicular development and reproductive function maintenance. Until now, functional studies on the Dmrt1 gene in sheep (Ovis aries) have been lacking. In this study, testis, heart, liver, spleen, lung, kidney and longissimus dorsi muscle tissues were collected from Small-Tail Han sheep at 0, 2, 5, 12 and 24months after birth (mab). Dmrt1 expression and cellular localization were detected in various testicular tissues by quantitative real time PCR (qRT-PCR), western blot and immunohistochemistry methods. The morphological structures of testicular tissues at different developmental stages were observed by hematoxylin & eosin (HE) staining. The Dmrt1 mRNA expression levels in 12 and 24 mab sheep were significantly higher than those in 0 and 2 mab sheep (P<0.05), and Dmrt1 protein expression showed a similar trend. The qRT-PCR results in various tissues at 12 mab showed that Dmrt1 mRNA was predominantly expressed in testes. Immunohistochemical staining in testes at different developmental stages showed that Dmrt1 protein immunoreactive responses were mainly localized in Sertoli cells and gonocytes at 0, 2 and 5 mab, while they were localized in spermatocytes, sperm cells and some spermatogonia and Sertoli cells at 12 and 24 mab. We speculate that the Dmrt1 gene plays a vital role in postnatal sheep spermatogenesis, perhaps by regulating the maturation and functional maintenance of Sertoli cells, the proliferation and differentiation of gonocytes in prepubertal sheep testes, and the mitosis and meiosis of germ cells in adult sheep, but the specific mechanisms underlying these phenomena must be further studied and verified.
[Mh] Termos MeSH primário: Ovinos/crescimento & desenvolvimento
Testículo/crescimento & desenvolvimento
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Proliferação Celular
Regulação da Expressão Gênica no Desenvolvimento
Masculino
Células de Sertoli/metabolismo
Diferenciação Sexual
Ovinos/genética
Ovinos/metabolismo
Espermatócitos/citologia
Espermatócitos/metabolismo
Espermatogênese
Testículo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Transcription Factors)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  7 / 3593 MEDLINE  
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[PMID]:28444885
[Au] Autor:Yin J; Ni B; Liao WG; Gao YQ
[Ad] Endereço:Department of Pathophysiology and High Altitude Pathology/Key Laboratory of High Altitude Environment Medicine (Third Military Medical University), Ministry of Education/Key Laboratory of High Altitude Medicine, College of High Altitude Military Medicine, Third Military Medical University, Chongqing
[Ti] Título:Hypoxia-induced apoptosis of mouse spermatocytes is mediated by HIF-1α through a death receptor pathway and a mitochondrial pathway.
[So] Source:J Cell Physiol;233(2):1146-1155, 2018 Feb.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hypoxia in vivo induces oligozoospermia, azoospermia, and degeneration of the germinal epithelium, but the underlying molecular mechanism of this induction is not fully clarified. The aim of this study was to investigate the role of the death receptor pathway and the mitochondrial pathway in hypoxia-induced apoptosis of mouse GC-2spd (GC-2) cells and the relationship between HIF-1α and apoptosis of GC-2 cells induced by hypoxia. GC-2 cells were subjected to 1% oxygen for 48 hr. Apoptosis was detected by flow cytometry, TUNEL staining, LDH, caspase-3/8/9 in the absence and presence of HIF-1α siRNA. The protein levels of apoptosis-related markers were determined by Western blot in the presence and absence of HIF-1α siRNA. Mitochondrial transmembrane potential change was observed by in situ JC-1 staining. Cell viability was assessed upon treatment of caspase-8 and 9 inhibitors. The results indicated that hypoxia at 1% oxygen for 48 hr induced apoptosis of GC-2 cells. A prolonged exposure of GC-2 cells to hypoxic conditions caused downregulation of c-FLIP, D R and Bcl-2 and upregulation of DR , TRAIL, Fas, p53, and Bax, with an overproduction of caspase-3/8/9. Moreover, hypoxia at this level had an effect on mitochondrial depolarization. In addition, specific inhibitors of caspase-8/9 partially suppressed hypoxia-induced GC-2 cell apoptosis, and the anti-apoptotic effects of the caspase inhibitors were additive. Of note, HIF-1α knockdown attenuated hypoxia and induced apoptosis of GC-2 cells. In conclusion, our data suggest that the death receptor pathway and mitochondrial pathway, which are likely mediated by HIF-1α, contribute to hypoxia-induced GC-2 cell apoptosis.
[Mh] Termos MeSH primário: Apoptose
Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo
Mitocôndrias/metabolismo
Receptores do Ligante Indutor de Apoptose Relacionado a TNF/metabolismo
Transdução de Sinais
Espermatócitos/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Proteínas Reguladoras de Apoptose/genética
Proteínas Reguladoras de Apoptose/metabolismo
Inibidores de Caspase/farmacologia
Hipóxia Celular
Linhagem Celular
Subunidade alfa do Fator 1 Induzível por Hipóxia/genética
Masculino
Potencial da Membrana Mitocondrial
Camundongos
Mitocôndrias/patologia
Interferência de RNA
Transdução de Sinais/efeitos dos fármacos
Espermatócitos/efeitos dos fármacos
Espermatócitos/patologia
Fatores de Tempo
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (Caspase Inhibitors); 0 (Hif1a protein, mouse); 0 (Hypoxia-Inducible Factor 1, alpha Subunit); 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand); 0 (Tnfrsf10b protein, mouse)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.25974


  8 / 3593 MEDLINE  
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[PMID]:28982183
[Au] Autor:Brieño-Enríquez MA; Moak SL; Holloway JK; Cohen PE
[Ad] Endereço:Department of Biomedical Sciences and Center for Reproductive Genomics, Cornell University, Ithaca, New York, United States of America.
[Ti] Título:NIMA-related kinase 1 (NEK1) regulates meiosis I spindle assembly by altering the balance between α-Adducin and Myosin X.
[So] Source:PLoS One;12(10):e0185780, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:NIMA-related kinase 1 (NEK1) is a serine/threonine and tyrosine kinase that is highly expressed in mammalian germ cells. Mutations in Nek1 induce anemia, polycystic kidney and infertility. In this study we evaluated the role of NEK1 in meiotic spindle formation in both male and female gametes. Our results show that the lack of NEK1 provokes an abnormal organization of the meiosis I spindle characterized by elongated and/or multipolar spindles, and abnormal chromosome congression. The aberrant spindle structure is concomitant with the disruption in localization and protein levels of myosin X (MYO10) and α-adducin (ADD1), both of which are implicated in the regulation of spindle formation during mitosis. Interaction of ADD1 with MYO10 is dependent on phosphorylation, whereby phosphorylation of ADD1 enables its binding to MYO10 on mitotic spindles. Reduction in ADD1 protein in NEK1 mutant mice is associated with hyperphosphorylation of ADD1, thereby preventing the interaction with MYO10 during meiotic spindle formation. Our results reveal a novel regulatory role for NEK1 in the regulation of spindle architecture and function during meiosis.
[Mh] Termos MeSH primário: Proteínas de Ligação a Calmodulina/metabolismo
Meiose/fisiologia
Miosinas/metabolismo
Quinase 1 Relacionada a NIMA/fisiologia
Fuso Acromático/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Mutantes
Oócitos/ultraestrutura
Fosforilação
Espermatócitos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calmodulin-Binding Proteins); 0 (adducin); EC 2.7.11.1 (NIMA-Related Kinase 1); EC 2.7.11.1 (Nek1 protein, mouse); EC 3.6.4.1 (Myosins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185780


  9 / 3593 MEDLINE  
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[PMID]:28942922
[Au] Autor:Zelazowski MJ; Sandoval M; Paniker L; Hamilton HM; Han J; Gribbell MA; Kang R; Cole F
[Ad] Endereço:Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Smithville, TX 78957, USA.
[Ti] Título:Age-Dependent Alterations in Meiotic Recombination Cause Chromosome Segregation Errors in Spermatocytes.
[So] Source:Cell;171(3):601-614.e13, 2017 Oct 19.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Faithful chromosome segregation in meiosis requires crossover (CO) recombination, which is regulated to ensure at least one CO per homolog pair. We investigate the failure to ensure COs in juvenile male mice. By monitoring recombination genome-wide using cytological assays and at hotspots using molecular assays, we show that juvenile mouse spermatocytes have fewer COs relative to adults. Analysis of recombination in the absence of MLH3 provides evidence for greater utilization in juveniles of pathways involving structure-selective nucleases and alternative complexes, which can act upon precursors to generate noncrossovers (NCOs) at the expense of COs. We propose that some designated CO sites fail to mature efficiently in juveniles owing to inappropriate activity of these alternative repair pathways, leading to chromosome mis-segregation. We also find lower MutLγ focus density in juvenile human spermatocytes, suggesting that weaker CO maturation efficiency may explain why younger men have a higher risk of fathering children with Down syndrome.
[Mh] Termos MeSH primário: Envelhecimento
Segregação de Cromossomos
Meiose
Recombinação Genética
Espermatócitos/metabolismo
[Mh] Termos MeSH secundário: Animais
Aberrações Cromossômicas
Reparo do DNA
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos DBA
Espermatócitos/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE


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[PMID]:28848076
[Au] Autor:Sciurano RB; De Luca G; Rahn IM; Solari AJ
[Ad] Endereço:2da. U.A. Biología Celular, Histología, Embriología y Genética, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
[Ti] Título:The XY Body of the Cat (Felis catus): Structural Differentiations and Protein Immunolocalization.
[So] Source:Cytogenet Genome Res;152(3):137-147, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The heteromorphic X and Y chromosomes behave in a special way in mammalian spermatocytes; they form the XY body and synapse only partially. The aim of this article was to study the origin and the role of the special differentiations in the XY pair of the domestic cat during pachytene by analyzing its fine structural characteristics and the immunolocalization of the main meiotic proteins SYCP3, SYCP1, SYCE3, SMC3, γ-H2AX, BRCA1, H3K27me3, and MLH1. The cat XY body shows particularly striking structures: an extreme degree of axial fibrillation in late pachynema and a special location of SYCP3-containing fibrils, bridging different regions of the main X axis, as well as one bridge at the inner end of the pairing region that colocalizes with the single mandatory MLH1 focus. There are sequential changes, first bullous expansions, then subdivision into fibrils, all involving axial thickening. The chromatin of the XY body presents the usual features of meiotic sex chromosome inactivation. An analysis of the XY body of many eutherians and metatherians suggests that axial thickenings are primitive features. The sequential changes in the mass and location of SYCP3-containing fibers vary among the clades because of specific processes of axial assembly/disassembly occurring in different species.
[Mh] Termos MeSH primário: Gatos/genética
Proteínas Nucleares/metabolismo
Estágio Paquíteno/genética
Complexo Sinaptonêmico/metabolismo
Cromossomo X/metabolismo
Cromossomo X/ultraestrutura
Cromossomo Y/metabolismo
Cromossomo Y/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Proteína BRCA1/genética
Proteína BRCA1/metabolismo
Cromatina/metabolismo
Cromatina/ultraestrutura
Histonas/genética
Histonas/metabolismo
Masculino
Microscopia de Fluorescência
Proteína 1 Homóloga a MutL/genética
Proteína 1 Homóloga a MutL/metabolismo
Espermatócitos/metabolismo
Complexo Sinaptonêmico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BRCA1 Protein); 0 (Chromatin); 0 (Histones); 0 (Nuclear Proteins); EC 3.6.1.3 (MutL Protein Homolog 1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1159/000479569



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