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Pesquisa : A05.360.490.890.900 [Categoria DeCS]
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[PMID]:29331483
[Au] Autor:Liu X; Li Z; Wang B; Zhu H; Liu Y; Qi J; Zhang Q
[Ad] Endereço:Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, 266003 Qingdao, Shandong, China.
[Ti] Título:GATA4 is a transcriptional regulator of R-spondin1 in Japanese flounder (Paralichthys olivaceus).
[So] Source:Gene;648:68-75, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:GATA4 is a well-known transcription factor of the GATA family implicated in regulation of sex determination and gonadal development in mammals. In this study, we cloned the full-length cDNA of Paralichthys olivaceus gata4 (Po-gata4). Phylogenetic, gene structure, and synteny analysis showed that Po-GATA4 is homologous to GATA4 of teleost and tetrapod. Po-gata4 transcripts were detected in Sertoli cells, spermatogonia, oogonia and oocytes, with higher transcript levels overall in the testis than the ovary. The promoter region of P. olivaceus R-spondin1was found to contain a GATA4-binding motif. Results of CBA (cleaved amplified polymorphic sequence-based binding assay) indicated that GATA4 could indeed bind to the promoter sequence of R-spondin1. Moreover, human GATA4 recombinant protein could upregulate R-spondin1 in P. olivaceus ovary cells and FBCs (flounder brain cell line). In FBCs, overexpression of Po-gata4 resulted in elevated transcript levels of R-spondin1. Taken together, our results indicate that Po-GATA4 is involved in gonadal development by regulating R-spondin1 expression.
[Mh] Termos MeSH primário: Embrião não Mamífero/metabolismo
Proteínas de Peixes/genética
Linguado/genética
Fator de Transcrição GATA4/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Linhagem Celular
Embrião não Mamífero/citologia
Embrião não Mamífero/embriologia
Feminino
Proteínas de Peixes/classificação
Proteínas de Peixes/metabolismo
Linguado/embriologia
Linguado/metabolismo
Fator de Transcrição GATA4/classificação
Fator de Transcrição GATA4/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Masculino
Oogônios/citologia
Oogônios/metabolismo
Filogenia
Regiões Promotoras Genéticas/genética
Ligação Proteica
Espermatogônias/citologia
Espermatogônias/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (GATA4 Transcription Factor)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180115
[St] Status:MEDLINE


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[PMID]:29293683
[Au] Autor:Kawai Y; Oda A; Kanai Y; Goitsuka R
[Ad] Endereço:Division of Development and Aging, Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba, Japan.
[Ti] Título:Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.
[So] Source:PLoS One;13(1):e0190702, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PKnox1 (also known as Prep1) belongs to the TALE family of homeodomain transcription factors that are critical for regulating growth and differentiation during embryonic and postnatal development in vertebrates. We demonstrate here that PKnox1 is required for adult spermatogenesis in a germ cell-intrinsic manner. Tamoxifen-mediated PKnox1 loss in the adult testes, as well as its germ cell-specific ablation, causes testis hypotrophy with germ cell apoptosis and, as a consequence, compromised spermatogenesis. In PKnox1-deficient testes, spermatogenesis was arrested at the c-Kit+ spermatogonia stage, with a complete loss of the meiotic spermatocytes, and was accompanied by compromised differentiation of the c-Kit+ spermatogonia. Taken together, these results indicate that PKnox1 is a critical regulator of maintenance and subsequent differentiation of the c-Kit+ stage of spermatogonia in the adult testes.
[Mh] Termos MeSH primário: Proteínas de Homeodomínio/fisiologia
Espermatogênese/fisiologia
Espermatozoides/metabolismo
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Espermatogônias/citologia
Testículo/citologia
Testículo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Homeodomain Proteins); 0 (Pknox1 protein, mouse)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190702


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[PMID]:28465413
[Au] Autor:Wu FJ; Lin TY; Sung LY; Chang WF; Wu PC; Luo CW
[Ad] Endereço:Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei 112, Taiwan.
[Ti] Título:BMP8A sustains spermatogenesis by activating both SMAD1/5/8 and SMAD2/3 in spermatogonia.
[So] Source:Sci Signal;10(477), 2017 May 02.
[Is] ISSN:1937-9145
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mutation in either of the genes encoding bone morphogenetic protein (BMP) 8A or 8B ( or ) causes postnatal depletion of spermatogonia in mice. We found that , but not , was expressed predominantly in the neonatal mouse spermatogonia. Although most BMPs induce activation of SMADs 1, 5, and 8 (SMAD1/5/8), but not SMADs 2 and 3 (SMAD2/3), we found that BMP8A induced signaling through both sets of transcription factors. In undifferentiated mouse spermatogonia, BMP8A activated SMAD1/5/8 through receptor complexes formed by ALK3 and either ACVR2A or BMPR2 and activated SMAD2/3 through receptor complexes formed by ALK5 and ACVR2A, ACVR2B, or TGFBR2. Signaling through SMAD2/3 promoted the proliferation of germ cells, whereas that through SMAD1/5/8 directed the subsequent differentiation of spermatogonia. BMP8A promoted spermatogenesis in cultured mouse testis explants, and the resulting spermatids were functionally competent for fertilization. These results suggest that the dual role of BMP8A in promoting proliferation and differentiation of spermatogonia may be exploited clinically to treat male infertility.
[Mh] Termos MeSH primário: Proteínas Morfogenéticas Ósseas/metabolismo
Diferenciação Celular
Proteínas Smad Reguladas por Receptor/metabolismo
Espermatogênese/fisiologia
Espermatogônias/citologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos ICR
Transdução de Sinais
Espermatogônias/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BMP8A protein, human); 0 (Bmp8a protein, mouse); 0 (Bone Morphogenetic Proteins); 0 (Smad Proteins, Receptor-Regulated)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:29331479
[Au] Autor:Zhao C; Zhu W; Yin S; Cao Q; Zhang H; Wen X; Zhang G; Xie W; Chen S
[Ad] Endereço:College of Life Sciences, Key Laboratory of Biodiversity and Biotechnology of Jiangsu Province, Nanjing Normal University, Nanjing, Jiangsu 210023, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China.
[Ti] Título:Molecular characterization and expression of Piwil1 and Piwil2 during gonadal development and treatment with HCG and LHRH-A in Odontobutis potamophila.
[So] Source:Gene;647:181-191, 2018 Mar 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Piwi proteins play an important regulatory role in germ cell division during gametogenesis and gonad development. In order to understand the function of Piwi genes in the reproductive process of the dark sleeper, we identified and characterized Piwil1 and Piwil2 from gonad tissue. The tissue distribution demonstrated that Piwils were highly expressed in the gonad of the dark sleeper. During gonad development, higher expression was observed in stage I of both the testes and ovaries than in subsequent stages at mRNA and protein levels. The results of immunohistochemistry demonstrated that Piwils were predominantly distributed in the spermatogonia, spermatocytes, and early oocytes. When treated with the HPG axis hormone (HCG and LHRH-A2), the expression of Piwils was significantly decreased in the testes and ovaries at mRNA and protein levels. All of these results indicated that Piwils play a vital role in gonad development and gametogenesis. Our findings provide valuable evidence to further clarify the underlying modulation mechanism of Piwils in teleosts.
[Mh] Termos MeSH primário: Proteínas Argonauta/genética
Gonadotropina Coriônica/farmacologia
Regulação da Expressão Gênica no Desenvolvimento/genética
Hormônio Liberador de Gonadotropina/farmacologia
Gônadas/efeitos dos fármacos
Perciformes/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Gametogênese/efeitos dos fármacos
Gametogênese/genética
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Masculino
Oócitos/efeitos dos fármacos
Filogenia
RNA Mensageiro/genética
Espermatócitos/efeitos dos fármacos
Espermatogônias/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Argonaute Proteins); 0 (Chorionic Gonadotropin); 0 (RNA, Messenger); 33515-09-2 (Gonadotropin-Releasing Hormone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180115
[St] Status:MEDLINE


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[PMID]:28745623
[Au] Autor:Lima AC; Jung M; Rusch J; Usmani A; Lopes AM; Conrad DF
[Ad] Endereço:Department of Genetics, Washington University School of Medicine; Graduate Program in Areas of Basic and Applied Biology (GABBA), Abel Salazar Institute of Biomedical Sciences, University of Porto; Instituto de Investigação e Inovação em Saúde, University of Porto; IPATIMUP - Instituto de Patologia
[Ti] Título:A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry.
[So] Source:J Vis Exp;(125), 2017 Jul 12.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fluorescence-activated cell sorting (FACS) has been one of the methods of choice to isolate enriched populations of mammalian testicular germ cells. Currently, it allows the discrimination of up to 9 murine germ cell populations with high yield and purity. This high-resolution in discrimination and purification is possible due to unique changes in chromatin structure and quantity throughout spermatogenesis. These patterns can be captured by flow cytometry of male germ cells stained with fluorescent DNA-binding dyes such as Hoechst-33342 (Hoechst). Herein is a detailed description of a recently developed protocol to isolate mammalian testicular germ cells. Briefly, single cell suspensions are generated from testicular tissue by mechanical dissociation, double stained with Hoechst and propidium iodide (PI) and processed by flow cytometry. A serial gating strategy, including the selection of live cells (PI negative) with different DNA content (Hoechst intensity), is used during FACS sorting to discriminate up to 5 germ cell types. These include, with corresponding average purities (determined by microscopy evaluation): spermatogonia (66%), primary (71%) and secondary (85%) spermatocytes, and spermatids (90%), further separated into round (93%) and elongating (87%) subpopulations. Execution of the entire workflow is straightforward, allows the isolation of 4 cell types simultaneously with the appropriate FACS machine, and can be performed in less than 2 h. As reduced processing time is crucial to preserve the physiology of ex vivo cells, this method is ideal for downstream high-throughput studies of male germ cell biology. Moreover, a standardized protocol for multispecies purification of mammalian germ cells eliminates methodological sources of variables and allows a single set of reagents to be used for different animal models.
[Mh] Termos MeSH primário: Citometria de Fluxo/métodos
Células Germinativas/metabolismo
Espermatogênese/fisiologia
Testículo/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Germinativas/citologia
Masculino
Camundongos
Espermatogônias/citologia
Testículo/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.3791/55913


  6 / 3540 MEDLINE  
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[PMID]:29288727
[Au] Autor:Li Q; Yang H; He L; Wang Q
[Ad] Endereço:College of Ecological Engineering, Guizhou University of Engineering Science, College Road, Bijie City, Guizhou Province 551700, China.
[Ti] Título:Characterization of the Es-DDX52 involved in the spermatogonial mitosis and spermatid differentiation in Chinese mitten crab (Eriocheir sinensis).
[So] Source:Gene;646:106-119, 2018 Mar 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Spermatogenesis involves a series of process including exiting from the mitotic cell cycle, entry into meiosis, completion of complex differentiation programs, and producing spermatozoa. Expression of various genes in an ordered manner, and interactions between various genes and their protein products, primarily controlled at the post-transcriptional level with DEAD-box RNA helicases playing a crucial role in germ cell development, are required for production of fertile sperm. Many members of this family have been deeply studied in spermatogenesis, such as DDX3X, DDX25 and DDX4, but few data are available on DDX52. In this study, we analyzed the expression patterns of Es-DDX52, Es-DDX6, Es-Vasa and Es-XRN1 both at mRNA and protein levels in different tissues and during gonadal development. It showed that Es-vasa, Es-DDX6 and Es-Xrn1, components of cytoplasmic foci P-bodies, have the similar transcriptional expression pattern, while Es-DDX52 has the reverse tendency. Furthermore, Es-DDX6 and Es-XRN1 proteins have the same localization in testicular tissues. Es-DDX52 mainly distributed in the cytoplasm of spermatogonia, only localized in the nucleus of early and middle spermatid and shifted to pre-acrosome vesicle (later developed into apical cap and acrosome tube) at both mRNA and protein levels. These results indicated that Es-DDX52 may participate in regulation of P-bodies and microtubules by Es-XRN1, and involved in the mitosis of spermatonia and spermatid differentiation.
[Mh] Termos MeSH primário: Braquiúros/metabolismo
RNA Helicases DEAD-box/genética
RNA Helicases DEAD-box/metabolismo
Espermátides/citologia
Espermatogônias/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Artrópodes/metabolismo
Diferenciação Celular
Clonagem Molecular
RNA Helicases DEAD-box/química
Regulação da Expressão Gênica
Masculino
Mitose
Modelos Moleculares
Filogenia
Espermátides/metabolismo
Espermatogênese
Espermatogônias/metabolismo
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


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[PMID]:28461394
[Au] Autor:Kataruka S; Akhade VS; Kayyar B; Rao MRS
[Ad] Endereço:Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India.
[Ti] Título:Mrhl Long Noncoding RNA Mediates Meiotic Commitment of Mouse Spermatogonial Cells by Regulating Sox8 Expression.
[So] Source:Mol Cell Biol;37(14), 2017 Jul 15.
[Is] ISSN:1098-5549
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Long noncoding RNAs (lncRNAs) are important regulators of various biological processes, including spermatogenesis. Our previous studies have revealed the regulatory loop of RNA and Wnt signaling, where RNA negatively regulates Wnt signaling and gets downregulated upon Wnt signaling activation. This downregulation of RNA is important for the meiotic progression of spermatogonial cells. In our present study, we identified the transcription factor Sox8 as the regulatory link between RNA expression, Wnt signaling activation, and meiotic progression. In contrast to reports from other groups, we report the expression of Sox8 in germ cells and describe the molecular mechanism of regulation by RNA during differentiation of spermatogonial cells. Binding of RNA to the promoter is accompanied by the assembly of other regulatory factors involving Myc-Max-Mad transcription factors, corepressor Sin3a, and coactivator Pcaf. In the context of Wnt signaling, Sox8 directly regulates the expression of premeiotic and meiotic markers. Prolonged Wnt signaling activation in spermatogonial cells leads to changes in global chromatin architecture and a decrease in levels of stem cell markers.
[Mh] Termos MeSH primário: Diferenciação Celular/fisiologia
RNA Longo não Codificante/genética
Fatores de Transcrição SOXE/metabolismo
Espermatogênese/fisiologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Cromatina/metabolismo
Masculino
Camundongos
Regiões Promotoras Genéticas/genética
Espermatogônias/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (RNA, Long Noncoding); 0 (SOXE Transcription Factors); 0 (Sox8 protein, mouse)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:28461596
[Au] Autor:Carrieri C; Comazzetto S; Grover A; Morgan M; Buness A; Nerlov C; O'Carroll D
[Ad] Endereço:MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH16 4UU, Scotland, UK.
[Ti] Título:A transit-amplifying population underpins the efficient regenerative capacity of the testis.
[So] Source:J Exp Med;214(6):1631-1641, 2017 Jun 05.
[Is] ISSN:1540-9538
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The spermatogonial stem cell (SSC) that supports spermatogenesis throughout adult life resides within the GFRα1-expressing A type undifferentiated spermatogonia. The decision to commit to spermatogenic differentiation coincides with the loss of GFRα1 and reciprocal gain of Ngn3 (Neurog3) expression. Through the analysis of the piRNA factor ( ), we identify a novel population of Ngn3-expressing spermatogonia that are essential for efficient testicular regeneration after injury. Depletion of -expressing cells results in a transient impact on testicular homeostasis, with this population behaving strictly as transit amplifying cells under homeostatic conditions. However, upon injury, -expressing cells are essential for the efficient regenerative capacity of the testis, and also display facultative stem activity in transplantation assays. In summary, the mouse testis has adopted a regenerative strategy to expand stem cell activity by incorporating a transit-amplifying population to the effective stem cell pool, thus ensuring rapid and efficient tissue repair.
[Mh] Termos MeSH primário: Regeneração/fisiologia
Testículo/fisiologia
[Mh] Termos MeSH secundário: Animais
Proteínas Argonauta/metabolismo
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Diferenciação Celular
Homeostase
Masculino
Camundongos
Proteínas do Tecido Nervoso/metabolismo
Proteínas Proto-Oncogênicas c-kit/metabolismo
Espermatogênese
Espermatogônias/citologia
Espermatogônias/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Argonaute Proteins); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Nerve Tissue Proteins); 0 (Neurog3 protein, mouse); 0 (PIWIL4 protein, mouse); EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171205
[Lr] Data última revisão:
171205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1084/jem.20161371


  9 / 3540 MEDLINE  
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[PMID]:29016690
[Au] Autor:Wu GC; Li HW; Tey WG; Lin CJ; Chang CF
[Ad] Endereço:Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
[Ti] Título:Expression profile of amh/Amh during bi-directional sex change in the protogynous orange-spotted grouper Epinephelus coioides.
[So] Source:PLoS One;12(10):e0185864, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gonadal differentiation is tightly regulated by the initial sex determining gene and the downstream sex-related genes in vertebrates. However, sex change in fish can alter the sexual fate from one sex to the other. Chemical-induced maleness in the protogynous orange-spotted grouper is transient, and a reversible sex change occurs after the chemical treatment is withdrawn. We used these characteristics to study Amh signaling during bi-directional sex change in the grouper. We successfully induced the female-to-male sex change by chemical (aromatase inhibitor, AI, or methyltestosterone, MT) treatment. A dormant gonad (a low proliferation rate of early germ cells and no characteristics of both sexes) was found during the transient phase of reversible male-to-female sex change after the withdrawal of chemical administration. Our results showed that amh (anti-mullerian hormone) and its receptor amhr2 (anti-mullerian hormone receptor type 2) were significantly increased in the gonads during the process of female-to-male sex change. Amh is expressed in the Sertoli cells surrounding the type A spermatogonia in the female-to-male grouper. Male-related gene (dmrt1 and sox9) expression was immediately decreased in MT-terminated males during the reversible male-to-female sex change. However, Amh expression was found in the surrounding cells of type A spermatogonia-like cells during the transient phase of reversible male-to-female sex change. This phenomenon is correlated with the dormancy of type A spermatogonia-like cells. Thus, Amh signaling is suggested to play roles in regulating male differentiation during the female-to-male sex change and in inhibiting type-A spermatogonia-like cell proliferation/differentiation during the reversible male-to-female sex change. We suggest that Amh signaling might play dual roles during bi-directional sex change in grouper.
[Mh] Termos MeSH primário: Hormônio Antimülleriano/genética
Inibidores da Aromatase/farmacologia
Regulação da Expressão Gênica no Desenvolvimento
Metiltestosterona/farmacologia
Receptores de Peptídeos/genética
Receptores de Fatores de Crescimento Transformadores beta/genética
Diferenciação Sexual/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Hormônio Antimülleriano/metabolismo
Feminino
Masculino
Ovário/citologia
Ovário/efeitos dos fármacos
Ovário/metabolismo
Perciformes
Receptores de Peptídeos/metabolismo
Receptores de Fatores de Crescimento Transformadores beta/metabolismo
Fatores de Transcrição SOX9/genética
Fatores de Transcrição SOX9/metabolismo
Células de Sertoli/citologia
Células de Sertoli/efeitos dos fármacos
Células de Sertoli/metabolismo
Processos de Determinação Sexual
Diferenciação Sexual/genética
Transdução de Sinais
Espermatogônias/citologia
Espermatogônias/efeitos dos fármacos
Espermatogônias/metabolismo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aromatase Inhibitors); 0 (DMRT1 protein); 0 (Receptors, Peptide); 0 (Receptors, Transforming Growth Factor beta); 0 (SOX9 Transcription Factor); 0 (Transcription Factors); 0 (anti-Mullerian hormone receptor); 80497-65-0 (Anti-Mullerian Hormone); V9EFU16ZIF (Methyltestosterone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185864


  10 / 3540 MEDLINE  
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[PMID]:28935708
[Au] Autor:Jan SZ; Vormer TL; Jongejan A; Röling MD; Silber SJ; de Rooij DG; Hamer G; Repping S; van Pelt AMM
[Ad] Endereço:Center for Reproductive Medicine, Amsterdam Research Institute Reproduction and Development, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
[Ti] Título:Unraveling transcriptome dynamics in human spermatogenesis.
[So] Source:Development;144(20):3659-3673, 2017 10 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spermatogenesis is a dynamic developmental process that includes stem cell proliferation and differentiation, meiotic cell divisions and extreme chromatin condensation. Although studied in mice, the molecular control of human spermatogenesis is largely unknown. Here, we developed a protocol that enables next-generation sequencing of RNA obtained from pools of 500 individually laser-capture microdissected cells of specific germ cell subtypes from fixed human testis samples. Transcriptomic analyses of these successive germ cell subtypes reveals dynamic transcription of over 4000 genes during human spermatogenesis. At the same time, many of the genes encoding for well-established meiotic and post-meiotic proteins are already present in the pre-meiotic phase. Furthermore, we found significant cell type-specific expression of post-transcriptional regulators, including expression of 110 RNA-binding proteins and 137 long non-coding RNAs, most of them previously not linked to spermatogenesis. Together, these data suggest that the transcriptome of precursor cells already contains the genes necessary for cellular differentiation and that timely translation controlled by post-transcriptional regulators is crucial for normal development. These established transcriptomes provide a reference catalog for further detailed studies on human spermatogenesis and spermatogenic failure.
[Mh] Termos MeSH primário: Espermatogênese
Espermatozoides/citologia
Transcriptoma
[Mh] Termos MeSH secundário: Adulto
Animais
Biópsia
Diferenciação Celular
Cromatina/química
Regulação da Expressão Gênica no Desenvolvimento
Seres Humanos
Microdissecção e Captura a Laser
Masculino
Meiose
Camundongos
Meia-Idade
Família Multigênica
RNA Mensageiro/metabolismo
Proteínas de Ligação a RNA/metabolismo
Espermatogônias/citologia
Testículo/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chromatin); 0 (RNA, Messenger); 0 (RNA-Binding Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE
[do] DOI:10.1242/dev.152413



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