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Pesquisa : A08.675.256.500 [Categoria DeCS]
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  1 / 8928 MEDLINE  
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[PMID]:28464514
[Au] Autor:Cintora P; Arikkath J; Kandel M; Popescu G; Best-Popescu C
[Ad] Endereço:Cellular Neuroscience and Imaging Laboratory, Department of Bioengineering, University of Illinois at Urbana-Champaign, 208 North Wright Street, Urbana, Illinois, 61801.
[Ti] Título:Cell density modulates intracellular mass transport in neural networks.
[So] Source:Cytometry A;91(5):503-509, 2017 May.
[Is] ISSN:1552-4930
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In order to fully understand brain connectivity and elucidate the mechanisms involved in central nervous system disease, the field of neuroscience depends on quantitative studies of neuronal structure and function. Cell morphology and neurite (axonal and dendritic) arborization are typically studied by immunohistochemical and fluorescence techniques. However, dry mass content and intracellular mass transport rates have largely been under-investigated given the inherent difficulties in their measurement. Here, spatial light interference microscopy (SLIM) and dispersion-relation phase spectroscopy (DPS) were used to measure pathlength fluctuations that report on the dry mass and transport within cultured primary neurons across low, medium, and high cell density conditions. It was found that cell density (confluence) affects significantly both the growth rate and mass transport. The analysis method is label-free and does not require neuronal tracing, particle tracking, or neuron reconstruction. Since SLIM can upgrade any existing phase contrast microscope and the imaging and analysis are high-throughput, we anticipate that this approach will be embraced by neuroscientists for broad scale studies. © 2017 International Society for Advancement of Cytometry.
[Mh] Termos MeSH primário: Encéfalo/ultraestrutura
Contagem de Células/métodos
Microscopia de Interferência/métodos
Neurônios/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Redes Neurais (Computação)
Neuritos/ultraestrutura
Análise Espectral/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1002/cyto.a.23111


  2 / 8928 MEDLINE  
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[PMID]:29281625
[Au] Autor:Tsuchiya T; Fujii M; Matsuda N; Kunida K; Uda S; Kubota H; Konishi K; Kuroda S
[Ad] Endereço:Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.
[Ti] Título:System identification of signaling dependent gene expression with different time-scale data.
[So] Source:PLoS Comput Biol;13(12):e1005913, 2017 12.
[Is] ISSN:1553-7358
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cells decode information of signaling activation at a scale of tens of minutes by downstream gene expression with a scale of hours to days, leading to cell fate decisions such as cell differentiation. However, no system identification method with such different time scales exists. Here we used compressed sensing technology and developed a system identification method using data of different time scales by recovering signals of missing time points. We measured phosphorylation of ERK and CREB, immediate early gene expression products, and mRNAs of decoder genes for neurite elongation in PC12 cell differentiation and performed system identification, revealing the input-output relationships between signaling and gene expression with sensitivity such as graded or switch-like response and with time delay and gain, representing signal transfer efficiency. We predicted and validated the identified system using pharmacological perturbation. Thus, we provide a versatile method for system identification using data with different time scales.
[Mh] Termos MeSH primário: Expressão Gênica
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular/genética
Diferenciação Celular/fisiologia
Biologia Computacional
Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo
Cinética
Sistema de Sinalização das MAP Quinases
Modelos Biológicos
Neuritos/metabolismo
Células PC12
Ratos
Biologia de Sistemas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Cyclic AMP Response Element-Binding Protein)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pcbi.1005913


  3 / 8928 MEDLINE  
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[PMID]:29216449
[Au] Autor:Liu CC; Zhao N; Fu Y; Wang N; Linares C; Tsai CW; Bu G
[Ad] Endereço:Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA. Electronic address: liu.chiachen@mayo.edu.
[Ti] Título:ApoE4 Accelerates Early Seeding of Amyloid Pathology.
[So] Source:Neuron;96(5):1024-1032.e3, 2017 Dec 06.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Accumulation and aggregation of amyloid-ß (Aß) in the brain is an initiating step in the pathogenesis of Alzheimer's disease (AD). The ε4 allele of apolipoprotein E (apoE) gene is the strongest genetic risk factor for late-onset AD. Although there is strong evidence showing that apoE4 enhances amyloid pathology, it is not clear what the critical stage(s) is during amyloid development in which apoE4 has the strongest impact. Using apoE inducible mouse models, we show that increased expression of astrocytic apoE4, but not apoE3, during the seeding stage of amyloid development enhanced amyloid deposition and neuritic dystrophy in amyloid model mice. ApoE4, but not apoE3, significantly increased brain Aß half-life measured by in vivo microdialysis. Furthermore, apoE4 expression increased whereas apoE3 reduced amyloid-related gliosis in the mouse brains. Together, our results demonstrate that apoE4 has the greatest impact on amyloid during the seeding stage, likely by perturbing Aß clearance and enhancing Aß aggregation.
[Mh] Termos MeSH primário: Amiloidose/patologia
Apolipoproteína E4/farmacologia
[Mh] Termos MeSH secundário: Doença de Alzheimer/patologia
Amiloidose/genética
Animais
Apolipoproteína E3/farmacologia
Astrócitos/efeitos dos fármacos
Astrócitos/metabolismo
Astrócitos/patologia
Encéfalo/patologia
Técnicas de Introdução de Genes
Gliose/patologia
Seres Humanos
Camundongos
Camundongos Transgênicos
Neuritos/efeitos dos fármacos
Neuritos/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apolipoprotein E3); 0 (Apolipoprotein E4)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180121
[Lr] Data última revisão:
180121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE


  4 / 8928 MEDLINE  
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[PMID]:29183014
[Au] Autor:Rosso G; Young P; Shahin V
[Ad] Endereço:Institute of Physiology II, WWU Münster, Münster, Germany.
[Ti] Título:Mechanosensitivity of Embryonic Neurites Promotes Their Directional Extension and Schwann Cells Progenitors Migration.
[So] Source:Cell Physiol Biochem;44(4):1263-1270, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Migration of Schwann cells (SCs) progenitors and neurite outgrowth from embryonic dorsal root ganglions (DRGs) are two central events during the development of the peripheral nervous system (PNS). How these two enthralling events preceding myelination are promoted is of great relevance from basic research and clinical aspects alike. Recent evidence demonstrates that biophysical cues (extracellular matrix stiffness) and biochemical signaling act in concert to regulate PNS myelination. Microenvironment stiffness of SCs progenitors and embryonic neurites dynamically changes during development. METHODS: DRG explants were isolated from day 12.5 to 13.5 mice embryos and plated on laminin-coated substrates with varied stiffness values. After 4 days in culture and immunostaining with specific markers, neurite outgrowth pattern, SCs progenitors migration, and growth cone shape and advance were analyzed with confocal fluorescence microscopy. RESULTS: We found out that growing substrate stiffness promotes directional neurite outgrowth, SCs progenitors migration, growth cone advance and presumably axons fasciculation. CONCLUSIONS: DRG explants are in vitro models for the research of PNS development, myelination and regeneration. Consequently, we conclude the following: Our observations point out the importance of mechanosensitivity for the PNS. At the same time, they prompt the investigation of the important yet unclear links between PNS biomechanics and inherited neuropathies with myelination disorders such as Charcot-Marie-Tooth 1A and hereditary neuropathy with liability to pressure palsies. Finally, they encourage the consideration of mechanosensitivity in bioengineering of scaffolds to aid nerve regeneration after injury.
[Mh] Termos MeSH primário: Neuritos/metabolismo
Crescimento Neuronal/fisiologia
Estresse Mecânico
[Mh] Termos MeSH secundário: Animais
Movimento Celular
Células Cultivadas
Técnicas de Cocultura
Embrião de Mamíferos/citologia
Gânglios Espinais/citologia
Gânglios Espinais/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Microscopia Confocal
Células de Schwann/citologia
Células de Schwann/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1159/000485485


  5 / 8928 MEDLINE  
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[PMID]:28466808
[Au] Autor:Ghorabi MT; Aliaghaei A; Sadeghi Y; Shaerzadeh F; Rad AA; Mohamadi R; J Ebrahimi M
[Ad] Endereço:Anatomy and Cell Biology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
[Ti] Título:Evidence supporting neuroprotective effect of adipose derived stem cells on PC12 cells against oxidative stress induced by H2O2.
[So] Source:Cell Mol Biol (Noisy-le-grand);63(3):1-6, 2017 Mar 31.
[Is] ISSN:1165-158X
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Adipose-derived stem cells (ADSCs) are a population of cells derived from adipose tissue. ADSCs exhibit multilineage development potential and are able to secrete various factors, which influence adjacent cells. The present study examined the protective effect of ADSC's conditioned media (ADSC-CM) on PC12 cells exposed to H2O2, an oxidative injury model. After isolation, ADSCs were cultured and their osteogenic and adipogenic differentiation confirmed. Then, PC12 cells were co-treated with ADSC-CM and H2O2. Next, the effects of ADSC-CM on neurite outgrowth and cell differentiation in the presence of H2O2 were determined. Moreover, cell viability and apoptotic cell death percentage were evaluated using MTT assay, Hoechst staining and flow cytometry. Our results indicated the neuroprotective effects of ADSC-CM on morphological and morphometrical properties of neuron-like PC12 cells. Additionally, the profound decrease in percentage of apoptotic cells confirmed the protective effects of conditioned media from ADSCs that may be related to the release of trophic factors.
[Mh] Termos MeSH primário: Tecido Adiposo/citologia
Peróxido de Hidrogênio/toxicidade
Fármacos Neuroprotetores/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Animais
Morte Celular/efeitos dos fármacos
Forma Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Cromatina/metabolismo
Meios de Cultivo Condicionados/farmacologia
Citoproteção/efeitos dos fármacos
DNA/metabolismo
Masculino
Neuritos/efeitos dos fármacos
Neuritos/metabolismo
Células PC12
Ratos
Células-Tronco/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (Culture Media, Conditioned); 0 (Neuroprotective Agents); 9007-49-2 (DNA); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.14715/cmb/2017.63.3.1


  6 / 8928 MEDLINE  
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[PMID]:28449923
[Au] Autor:Berkovitch Y; Seliktar D
[Ad] Endereço:The Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 32000, Israel; The Interdisciplinary Program for Biotechnology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
[Ti] Título:Semi-synthetic hydrogel composition and stiffness regulate neuronal morphogenesis.
[So] Source:Int J Pharm;523(2):545-555, 2017 May 25.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study describes the use of a set of protein-based biomaterials that allow us to explore the mechanism of cell-mediated 3-D invasion associated with peripheral nerve regeneration. Hydrogels made from poly(ethylene glycol) (PEG) conjugated extracellular matrix proteins, including fibrinogen, gelatin and albumin were compared in their ability to support the neurite extension and glial cell migration from dorsal root ganglion (DRG) as compared to PEG only hydrogel controls. The synthetic polymer in the system provides a cross-linked network with controlled mechanical properties and degradation, whereas the protein components provide the unique extracellular matrix (ECM) for controlling neuronal cell morphogenesis. A range of hydrogel compositions were found to support DRG cell outgrowth, based on the mechanical properties, density and proteolytic degradation of the matrix. The 3-D invasion and morphogenesis of newly grown neurites and glial cells in the different materials were characterized and correlated to the properties of the scaffolds. The DRG cell outgrowth was highly correlated with the density of different hydrogel compositions.
[Mh] Termos MeSH primário: Hidrogéis/química
Neuritos/fisiologia
Neuroglia/citologia
Neurônios/citologia
Engenharia Tecidual
Tecidos Suporte
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Embrião de Galinha
Proteínas da Matriz Extracelular/química
Polietilenoglicóis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Extracellular Matrix Proteins); 0 (Hydrogels); 30IQX730WE (Polyethylene Glycols)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  7 / 8928 MEDLINE  
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[PMID]:29176874
[Au] Autor:Reyes-Corona D; Vázquez-Hernández N; Escobedo L; Orozco-Barrios CE; Ayala-Davila J; Moreno MG; Amaro-Lara ME; Flores-Martinez YM; Espadas-Alvarez AJ; Fernandez-Parrilla MA; Gonzalez-Barrios JA; Gutierrez-Castillo ME; González-Burgos I; Martinez-Fong D
[Ad] Endereço:Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Ciudad de México, México.
[Ti] Título:Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion.
[So] Source:PLoS One;12(11):e0188239, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The structural effect of neurturin (NRTN) on the nigrostriatal dopaminergic system in animals remains unknown, although NRTN has been shown to be effective in Parkinson's disease animal models. Herein, we aimed to demonstrate that NRTN overexpression in dopaminergic neurons stimulates both neurite outgrowths in the nigrostriatal pathway and striatal dendritic spines in aging rats with chronic 6-hydroxydopamine (6-OHDA) lesion. At week 12 after lesion, pTracer-mNRTN-His or pGreenLantern-1 plasmids were intranigrally transfected using the NTS-polyplex nanoparticles system. We showed that the transgenic expression in dopaminergic neurons remained until the end of the study (12 weeks). Only animals expressing NRTN-His showed recovery of tyrosine hydroxylase (TH)+ cells (28 ± 2%), their neurites (32 ± 2%) and the neuron-specific cytoskeletal marker ß-III-tubulin in the substantia nigra; striatal TH(+) fibers were also recovered (52 ± 3%), when compared to the healthy condition. Neurotensin receptor type 1 levels were also significantly recovered in the substantia nigra and striatum. Dopamine recovery was 70 ± 4% in the striatum and complete in the substantia nigra. The number of dendritic spines of striatal medium spiny neurons was also significantly increased, but the recovery was not complete. Drug-activated circling behavior decreased by 73 ± 2% (methamphetamine) and 89 ± 1% (apomorphine). Similar decrease was observed in the spontaneous motor behavior. Our results demonstrate that NRTN causes presynaptic and postsynaptic restoration of the nigrostriatal dopaminergic system after a 6-OHDA-induced chronic lesion. However, those improvements did not reach the healthy condition, suggesting that NRTN exerts lesser neurotrophic effects than other neurotrophic approaches.
[Mh] Termos MeSH primário: Neurônios Dopaminérgicos/metabolismo
Neurturina/metabolismo
Terminações Pré-Sinápticas/metabolismo
[Mh] Termos MeSH secundário: Animais
Corpo Estriado/metabolismo
Corpo Estriado/patologia
Citoesqueleto/metabolismo
Espinhas Dendríticas/metabolismo
Dopamina/metabolismo
Ensaio de Imunoadsorção Enzimática
Membro Anterior/fisiologia
Masculino
Camundongos
Neuritos/metabolismo
Oxidopamina
Ratos Wistar
Receptores de Neurotensina/metabolismo
Substância Negra/metabolismo
Substância Negra/patologia
Transfecção
Vibrissas/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurturin); 0 (Receptors, Neurotensin); 0 (neurotensin type 1 receptor); 8HW4YBZ748 (Oxidopamine); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188239


  8 / 8928 MEDLINE  
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[PMID]:28450116
[Au] Autor:Shida M; Mikami T; Tamura JI; Kitagawa H
[Ad] Endereço:Department of Biochemistry, Kobe Pharmaceutical University, Higashinada-ku, Kobe 658-8558, Japan.
[Ti] Título:A characteristic chondroitin sulfate trisaccharide unit with a sulfated fucose branch exhibits neurite outgrowth-promoting activity: Novel biological roles of fucosylated chondroitin sulfates isolated from the sea cucumber Apostichopus japonicus.
[So] Source:Biochem Biophys Res Commun;487(3):678-683, 2017 06 03.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chondroitin sulfate (CS) is a class of sulfated glycosaminoglycan (GAG) chains that consist of repeating disaccharide unit composed of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc). CS chains are found throughout the pericellular and extracellular spaces and contribute to the formation of functional microenvironments for numerous biological events. However, their structure-function relations remain to be fully characterized. Here, a fucosylated CS (FCS) was isolated from the body wall of the sea cucumber Apostichopus japonicus. Its promotional effects on neurite outgrowth were assessed by using isolated polysaccharides and the chemically synthesized FCS trisaccharide ß-D-GalNAc(4,6-O-disulfate) (1-4)[α-l-fucose (2,4-O-disulfate) (1-3)]-ß-D-GlcA. FCS polysaccharides contained the E-type disaccharide unit GlcA-GalNAc(4,6-O-disulfate) as a CS major backbone structure and carried distinct sulfated fucose branches. Despite their relatively lower abundance of E unit, FCS polysaccharides exhibited neurite outgrowth-promoting activity comparable to squid cartilage-derived CS-E polysaccharides, which are characterized by their predominant E units, suggesting potential roles of the fucose branch in neurite outgrowth. Indeed, the chemically synthesized FCS trisaccharide was as effective as CS-E tetrasaccharide in stimulating neurite elongation in vitro. In conclusion, FCS trisaccharide units with 2,4-O-disulfated fucose branches may provide new insights into understanding the structure-function relations of CS chains.
[Mh] Termos MeSH primário: Sulfatos de Condroitina/administração & dosagem
Neuritos/efeitos dos fármacos
Neuritos/fisiologia
Neurogênese/efeitos dos fármacos
Neurogênese/fisiologia
Pepinos-do-Mar/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Sulfatos de Condroitina/química
Relação Dose-Resposta a Droga
Fucose/química
Camundongos
Neuritos/ultraestrutura
Trissacarídeos/administração & dosagem
Trissacarídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Trisaccharides); 0 (fucosylated chondroitin sulfate); 28RYY2IV3F (Fucose); 9007-28-7 (Chondroitin Sulfates)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  9 / 8928 MEDLINE  
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[PMID]:29065138
[Au] Autor:Krishtal J; Bragina O; Metsla K; Palumaa P; Tõugu V
[Ad] Endereço:Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia.
[Ti] Título:In situ fibrillizing amyloid-beta 1-42 induces neurite degeneration and apoptosis of differentiated SH-SY5Y cells.
[So] Source:PLoS One;12(10):e0186636, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The progression of Alzheimer's disease is causatively linked to the accumulation of amyloid-ß aggregates in the brain, however, it is not clear how the amyloid aggregates initiate the death of neuronal cells. The in vitro toxic effects of amyloid peptides are most commonly examined using the human neuroblastoma derived SH-SY5Y cell line and here we show that differentiated neuron-like SH-SY5Y cells are more sensitive to amyloid peptides than non-differentiated cells, because the latter lack long neurites. Exogenous soluble amyloid-ß 1-42 covered cell bodies and whole neurites in differentiated cells with dense fibrils, causing neurite beading and fragmentation, whereas preformed amyloid-ß 1-42 fibrils had no toxic effects. Importantly, spontaneously fibrillizing amyloid-ß 1-42 peptide exhibited substantially higher cellular toxicity than amyloid-ß 1-40, which did not form fibrils under the experimental conditions. These results support the hypothesis that peptide toxicity is related to the active fibrillization process in the incubation mixture.
[Mh] Termos MeSH primário: Peptídeos beta-Amiloides/metabolismo
Apoptose
Diferenciação Celular
Neuritos
Fragmentos de Peptídeos/metabolismo
[Mh] Termos MeSH secundário: Fator Neurotrófico Derivado do Encéfalo/metabolismo
Linhagem Celular Tumoral
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amyloid beta-Peptides); 0 (Brain-Derived Neurotrophic Factor); 0 (Peptide Fragments); 0 (amyloid beta-protein (1-42))
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171025
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186636


  10 / 8928 MEDLINE  
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[PMID]:29049304
[Au] Autor:Lynch KJ; Skalli O; Sabri F
[Ad] Endereço:Dept. of Physics and Materials Science, University of Memphis, Memphis, Tennessee, United States of America.
[Ti] Título:Investigation of surface topography and stiffness on adhesion and neurites extension of PC12 cells on crosslinked silica aerogel substrates.
[So] Source:PLoS One;12(10):e0185978, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fundamental understanding and characterization of neural response to substrate topography is essential in the development of next generation biomaterials for nerve repair. Aerogels are a new class of materials with great potential as a biomaterial. In this work, we examine the extension of neurites by PC12 cells plated on matrigel-coated and collagen-coated mesoporous aerogel surfaces. We have successfully established the methodology for adhesion and growth of PC12 cells on polyurea crosslinked silica aerogels. Additionally, we have quantified neurite behaviors and compared their response on aerogel substrates with their behavior on tissue culture (TC) plastic, and polydimethylsiloxane (PDMS). We found that, on average, PC12 cells extend longer neurites on crosslinked silica aerogels than on tissue culture plastic, and, that the average number of neurites per cluster is lower on aerogels than on tissue culture plastic. Aerogels are an attractive candidate for future development of smart neural implants and the work presented here creates a platform for future work with this class of materials as a substrate for bioelectronic interfacing.
[Mh] Termos MeSH primário: Géis
Neuritos
Dióxido de Silício
[Mh] Termos MeSH secundário: Animais
Dimetilpolisiloxanos
Seres Humanos
Microscopia Eletrônica de Varredura
Células PC12
Plásticos
Ratos
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dimethylpolysiloxanes); 0 (Gels); 0 (Plastics); 63148-62-9 (baysilon); 7631-86-9 (Silicon Dioxide)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171020
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185978



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