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  1 / 19283 MEDLINE  
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[PMID]:27776979
[Au] Autor:Salman IM; Hildreth CM; Phillips JK
[Ad] Endereço:Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW, Australia. Electronic address: ibraheem_muhammed@yahoo.com.
[Ti] Título:Chronic kidney disease impairs renal nerve and haemodynamic reflex responses to vagal afferent input through a central mechanism.
[So] Source:Auton Neurosci;204:65-73, 2017 05.
[Is] ISSN:1872-7484
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We investigated age- and sex-related changes in reflex renal sympathetic nerve activity (RSNA) and haemodynamic responses to vagal afferent stimulation in a rodent model of chronic kidney disease (CKD). Using anaesthetised juvenile (7-8weeks) and adult (12-13weeks) Lewis Polycystic Kidney (LPK) and Lewis control rats of either sex (n=63 total), reflex changes in RSNA, heart rate (HR) and mean arterial pressure (MAP) to vagal afferent stimulation (5-s train, 4.0V, 2.0-ms pulses, 1-16Hz) were measured. In all groups, stimulation of the vagal afferents below 16Hz produced frequency-dependent reductions in RSNA, HR and MAP, while a 16Hz stimulus produced an initial sympathoinhibition followed by sympathoexcitation. In juvenile LPK versus age-matched Lewis, sympathoinhibition was reduced when responses were expressed as % baseline (P<0.05), but not as microvolts, while bradycardic responses were greater. Reflex depressor responses were greater (P=0.015) only in juvenile female LPK. In adult LPK, reflex sympathoinhibition (%) was blunted (P<0.05), and an age-related decline apparent (when expressed as microvolts). Reflex reductions in HR and MAP were only diminished (P<0.05) in adult female LPK versus age-matched Lewis. Peak reflex sympathoexcitation at 16Hz did not differ between groups; however, area under the curve values were greater in the LPK versus Lewis (overall, 9±1 versus 19±3µVs, P<0.05) irrespective of age, suggestive of enhanced sympathoexcitatory drive in the LPK. Our data demonstrates a progressive deficit in the central processing of vagal afferent input and a differential sex influence on reflex regulation of autonomic function and blood pressure homeostasis in CKD.
[Mh] Termos MeSH primário: Hemodinâmica/fisiologia
Rim/inervação
Rim/fisiopatologia
Reflexo/fisiologia
Insuficiência Renal Crônica/fisiopatologia
Nervo Vago/fisiopatologia
[Mh] Termos MeSH secundário: Vias Aferentes/fisiopatologia
Animais
Área Sob a Curva
Pressão Sanguínea/fisiologia
Estudos de Coortes
Modelos Animais de Doenças
Feminino
Frequência Cardíaca/fisiologia
Masculino
Ratos Endogâmicos Lew
Especificidade da Espécie
Sistema Nervoso Simpático/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  2 / 19283 MEDLINE  
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[PMID]:29244457
[Au] Autor:Urakova MA; Bryndina IG
[Ti] Título:Water balance of lung and nitrogen oxide in blood at experimental autoimmune encephalomyelitis after capsaicin blockade of vagus nerve.
[So] Source:Patol Fiziol Eksp Ter;60(3):18-22, 2016 Jul-Sep.
[Is] ISSN:0031-2991
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The purpose of the research: To study the water balance of lung and NO level in blood in experimental autoimmune encephalomyelitis combined with capsaicin blockade of vagus nerve. Methods: Experiments were conducted on 47 adult (16-week-old) male rats weighing 220-280 g. To simulate the experimental autoimmune encephalomyelitis (EAE) rats were subcutaneously injected with encephalitogenic mixture in complete Freund's adjuvant (0.2 ml; the content of inactivated Mycobacterium tuberculosis was 5 mg/ml) at the rate of 100 mg of homologous spinal cord homogenate per animal. Сapsaicin blockade was performed by bilateral application of 50 uM capsaicin («Sigma¼) on the neck portions of vagus nerves. The animals were divided into 4 groups: intact rats - control group1; rats with EAE; rats with capsaicin application on vagus nerve + EAE; sham operated rats subjected to vagus nerves allocation without the subsequent capsaicin application + EAE - control group 2. The next parameters were detected: the content of nitric oxide in blood plasma; protein content in broncho-alveolar lavage fluid; lung water balance indices including the amount of total, extra- and intravascular fluid and blood supply of lungs, which were calculated based on wet and dry lung mass and the hemoglobin content in blood and lung tissue determined by hemiglobincyanide method. Results: It was found that EAE is accompanied by an increase of total fluid, extravascular fluid (EVF) and blood supply of lungs on the background of increasing content of nitric oxide in arterial (art) and venous (ven) blood. In EAE and its combination with bilateral capsaicin blockade of vagus nerve a strong negative correlation between the NOart / NOven coefficient and EVF amount was found out. The blockade of capsaicin-sensitive vagal afferents normalized lung water balance impaired in EAE and restored the levels of nitric oxide in blood plasma. Conclusion: The obtained results suggest that capsaicin-sensitive vagal afferents with NO-ergic mechanisms involvment take part in the development of pulmonary hyperhydration during experimental autoimmune encephalomyelitis.
[Mh] Termos MeSH primário: Capsaicina/efeitos adversos
Encefalomielite Autoimune Experimental
Pulmão
Óxidos de Nitrogênio
Nervo Vago
Equilíbrio Hidroeletrolítico
[Mh] Termos MeSH secundário: Animais
Capsaicina/farmacologia
Encefalomielite Autoimune Experimental/sangue
Encefalomielite Autoimune Experimental/imunologia
Pulmão/imunologia
Pulmão/metabolismo
Masculino
Óxidos de Nitrogênio/sangue
Óxidos de Nitrogênio/imunologia
Ratos
Equilíbrio Hidroeletrolítico/efeitos dos fármacos
Equilíbrio Hidroeletrolítico/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrogen Oxides); S07O44R1ZM (Capsaicin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE


  3 / 19283 MEDLINE  
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[PMID]:29245271
[Au] Autor:Tsuchihashi K; Yoshihiro T; Aikawa T; Nio K; Takayoshi K; Yokoyama T; Fukata M; Arita S; Ariyama H; Shimizu Y; Yoshida Y; Torisu T; Esaki M; Odashiro K; Kusaba H; Akashi K; Baba E
[Ad] Endereço:aDepartment of Medicine and Biosystemic Science, Graduate School of Medical SciencesbDepartment of Comprehensive Clinical Oncology, Faculty of Medical SciencescDepartment of Anesthesiology and Critical Care MedicinedDepartment of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
[Ti] Título:Metastatic esophageal cancer presenting as shock by injury of vagus nerve mimicking baroreceptor reflex: A case report.
[So] Source:Medicine (Baltimore);96(49):e8987, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Neurogenic shock is generally typified by spinal injury due to bone metastases in cancer patients, but continuous disturbance of the vagus nerve controlling the aortic arch baroreceptor can cause shock by a reflex response through the medulla oblongata. PATIENT CONCERNS: A 43-year-old woman with dysphagia presented to our hospital. Computed tomography showed a primary tumor adjacent to and surrounding half the circumference of the descending aorta, and multiple cervical lymph node metastases, including a 55 × 35-mm lymph node overlapping the root of the left vagus nerve. Squamous esophageal cancer (T4bN3M1, stage IV) was diagnosed. Whereas shock status initially appeared soon after left cervical pain, suggesting pain-induced neutrally-mediated syncope, sustained bradycardia and hypotension occurred even after alleviation of pain by opioids. DIAGNOSIS: Disturbance of the left vagus nerve associated with the aortic arch baroreceptor by a large left cervical lymph node metastasis was considered as the cause of shock, pathologically mimicking the baroreceptor reflex. INTERVENTIONS: Systemic steroid administration was performed, and radiotherapy for both the primary site and lymph node metastasis was started 2 days after initiating steroid treatment. OUTCOMES: Four days after initiating steroid administration, hypotension and bradycardia were improved and stable. LESSONS: Disturbance of the vagus nerve controlling the aortic arch baroreceptor should be kept in mind as a potential cause of neurogenic shock in cancer patients, through a pathological reflex mimicking the baroreceptor reflex.
[Mh] Termos MeSH primário: Carcinoma de Células Escamosas/diagnóstico
Carcinoma de Células Escamosas/patologia
Neoplasias Esofágicas/diagnóstico
Neoplasias Esofágicas/patologia
Pressorreceptores/fisiopatologia
Nervo Vago/fisiopatologia
[Mh] Termos MeSH secundário: Adulto
Carcinoma de Células Escamosas/terapia
Neoplasias Esofágicas/terapia
Esofagoscopia
Feminino
Seres Humanos
Metástase Linfática
Estadiamento de Neoplasias
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008987


  4 / 19283 MEDLINE  
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[PMID]:28982197
[Au] Autor:Wang J; Kollarik M; Ru F; Sun H; McNeil B; Dong X; Stephens G; Korolevich S; Brohawn P; Kolbeck R; Undem B
[Ad] Endereço:Department of Respiratory, Inflammatory, and Autoimmunity, MedImmune, LLC, Gaithersburg, Maryland, United States of America.
[Ti] Título:Distinct and common expression of receptors for inflammatory mediators in vagal nodose versus jugular capsaicin-sensitive/TRPV1-positive neurons detected by low input RNA sequencing.
[So] Source:PLoS One;12(10):e0185985, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Capsaicin-sensitive sensory C-fibers derived from vagal ganglia innervate the visceral organs, and respond to inflammatory mediators and noxious stimuli. These neurons play an important role in maintenance of visceral homeostasis, and contribute to the symptoms of visceral inflammatory diseases. Vagal sensory neurons are located in two ganglia, the jugular ganglia (derived from the neural crest), and the nodose ganglia (from the epibranchial placodes). The functional difference, especially in response to immune mediators, between jugular and nodose neurons is not fully understood. In this study, we microscopically isolated murine nodose and jugular capsaicin-sensitive / Trpv1-expressing C-fiber neurons and performed transcriptome profiling using ultra-low input RNA sequencing. RNAseq detected genes with significantly differential expression in jugular and nodose neurons, which were mostly involved in neural functions. Transcriptional regulators, including Cited1, Hoxb5 and Prdm12 showed distinct expression patterns in the two C-fiber neuronal populations. Common and specific expression of immune receptor proteins was characterized in each neuronal type. The expression of immune receptors that have received little or no attention from vagal sensory biologists is highlighted including receptors for certain chemokines (CXCLs), interleukins (IL-4) and interferons (IFNα, IFNγ). Stimulation of immune receptors with their cognate ligands led to activation of the C-fibers in isolated functional assays.
[Mh] Termos MeSH primário: Capsaicina/farmacologia
Mediadores da Inflamação/metabolismo
Neurônios/metabolismo
Gânglio Nodoso/metabolismo
Receptores de Superfície Celular/metabolismo
Análise de Sequência de RNA/métodos
Canais de Cátion TRPV/metabolismo
Nervo Vago/metabolismo
[Mh] Termos MeSH secundário: Animais
Cálcio/metabolismo
Camundongos
Neurônios/efeitos dos fármacos
Gânglio Nodoso/citologia
Nervo Vago/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Inflammation Mediators); 0 (Receptors, Cell Surface); 0 (TRPV Cation Channels); 0 (TRPV1 protein, mouse); S07O44R1ZM (Capsaicin); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185985


  5 / 19283 MEDLINE  
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[PMID]:28928093
[Au] Autor:Sawada Y; Izumida Y; Takeuchi Y; Aita Y; Wada N; Li E; Murayama Y; Piao X; Shikama A; Masuda Y; Nishi-Tatsumi M; Kubota M; Sekiya M; Matsuzaka T; Nakagawa Y; Sugano Y; Iwasaki H; Kobayashi K; Yatoh S; Suzuki H; Yagyu H; Kawakami Y; Kadowaki T; Shimano H; Yahagi N
[Ad] Endereço:Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan; Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan.
[Ti] Título:Effect of sodium-glucose cotransporter 2 (SGLT2) inhibition on weight loss is partly mediated by liver-brain-adipose neurocircuitry.
[So] Source:Biochem Biophys Res Commun;493(1):40-45, 2017 Nov 04.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sodium-glucose cotransporter 2 (SGLT2) inhibitors have both anti-diabetic and anti-obesity effects. However, the precise mechanism of the anti-obesity effect remains unclear. We previously demonstrated that the glycogen depletion signal triggers lipolysis in adipose tissue via liver-brain-adipose neurocircuitry. In this study, therefore, we investigated whether the anti-obesity mechanism of SGLT2 inhibitor is mediated by this mechanism. Diet-induced obese mice were subjected to hepatic vagotomy (HVx) or sham operation and loaded with high fat diet containing 0.015% tofogliflozin (TOFO), a highly selective SGLT2 inhibitor, for 3 weeks. TOFO-treated mice showed a decrease in fat mass and the effect of TOFO was attenuated in HVx group. Although both HVx and sham mice showed a similar level of reduction in hepatic glycogen by TOFO treatment, HVx mice exhibited an attenuated response in protein phosphorylation by protein kinase A (PKA) in white adipose tissue compared with the sham group. As PKA pathway is known to act as an effector of the liver-brain-adipose axis and activate triglyceride lipases in adipocytes, these results indicated that SGLT2 inhibition triggered glycogen depletion signal and actuated liver-brain-adipose axis, resulting in PKA activation in adipocytes. Taken together, it was concluded that the effect of SGLT2 inhibition on weight loss is in part mediated via the liver-brain-adipose neurocircuitry.
[Mh] Termos MeSH primário: Tecido Adiposo/fisiologia
Compostos Benzidrílicos/administração & dosagem
Encéfalo/fisiologia
Glucosídeos/administração & dosagem
Fígado/fisiologia
Transportador 2 de Glucose-Sódio/antagonistas & inibidores
Transportador 2 de Glucose-Sódio/metabolismo
Perda de Peso/fisiologia
[Mh] Termos MeSH secundário: Tecido Adiposo/efeitos dos fármacos
Tecido Adiposo/inervação
Animais
Fármacos Antiobesidade/administração & dosagem
Encéfalo/efeitos dos fármacos
Fígado/efeitos dos fármacos
Fígado/inervação
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Vagotomia
Nervo Vago/efeitos dos fármacos
Nervo Vago/fisiologia
Nervo Vago/cirurgia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (6-((4-ethylphenyl)methyl)-3',4',5',6'-tetrahydro-6'-(hydroxymethyl)spiro(isobenzofuran-1(3H),2'-(2H)pyran)-3',4',5'-triol); 0 (Anti-Obesity Agents); 0 (Benzhydryl Compounds); 0 (Glucosides); 0 (Slc5a2 protein, mouse); 0 (Sodium-Glucose Transporter 2)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE


  6 / 19283 MEDLINE  
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[PMID]:28865207
[Au] Autor:Sienkiewicz W; Dudek A; Zacharko-Siembida A; Marszalek M
[Ad] Endereço:.
[Ti] Título:Immunohistochemical characterization of the jugular (superior vagal) ganglion in the pig.
[So] Source:Pol J Vet Sci;20(2):377-385, 2017 Mar 01.
[Is] ISSN:1505-1773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The study was carried out on three 4-month old female pigs. All the animals were deeply anesthetized and transcardially perfused with 4% buffered paraformaldehyde (pH 7.4). Left and right superior vagal ganglia (SVG) were collected and processed for immunofluorescence labeling method. The preparations were examined under a Zeiss LSM 710 confocal microscope equipped with adequate filter block. Neurons forming SVG were round or oval in shape with a round nucleus in the center. The majority of them (52%) were medium (M) (31-50 µm in diameter) while 7% and 41% were small (S) (up to 30µm in diameter) or large (L) (above 50 µm in diameter) in size, respectively. Double-labeling immunofluorescence revealed that SVG neurons stained for CGRP (approx. 57%; among them 37%, 9% and 54% were M, S and L in size, respectively), SP (14.5%; 72.4% M, 3.4% S, 24.2% L), VACHT (26%; 63% M, 24% S and 13% L), GAL (14%; 57% M, 29% S, 14% L), NPY (12%; 53% M, 12% S, 35% L), Met-Enk (5%; 40% M, 6% S and 54% L), PACAP (15%; 52% M, 24% S and 24% L), VIP (6.3%; 67% M, 8% S and 25% L), and NOS-positive (6%; 31% M and 69% L). The most abundant populations of intraganglionic nerve fibers were those which stained for CGRP or GAL, whereas only single SP-, PACAP- or Met-ENK-positive nerve terminals were observed.
[Mh] Termos MeSH primário: Gânglios/citologia
Gânglios/fisiologia
Imuno-Histoquímica/veterinária
Neurônios/fisiologia
Suínos/fisiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Nervo Vago/anatomia & histologia
Nervo Vago/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE


  7 / 19283 MEDLINE  
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[PMID]:28803148
[Au] Autor:Calderón-Garcidueñas L; Reynoso-Robles R; Pérez-Guillé B; Mukherjee PS; Gónzalez-Maciel A
[Ad] Endereço:The University of Montana, Missoula, MT 59812, USA; Universidad del Valle de México, Mexico City 14370, Mexico. Electronic address: lilian.calderon-garciduenas@umontana.edu.
[Ti] Título:Combustion-derived nanoparticles, the neuroenteric system, cervical vagus, hyperphosphorylated alpha synuclein and tau in young Mexico City residents.
[So] Source:Environ Res;159:186-201, 2017 11.
[Is] ISSN:1096-0953
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mexico City (MC) young residents are exposed to high levels of fine particulate matter (PM ), have high frontal concentrations of combustion-derived nanoparticles (CDNPs), accumulation of hyperphosphorylated aggregated α-synuclein (α-Syn) and early Parkinson's disease (PD). Swallowed CDNPs have easy access to epithelium and submucosa, damaging gastrointestinal (GI) barrier integrity and accessing the enteric nervous system (ENS). This study is focused on the ENS, vagus nerves and GI barrier in young MC v clean air controls. Electron microscopy of epithelial, endothelial and neural cells and immunoreactivity of stomach and vagus to phosphorylated É‘-synuclein Ser129 and Hyperphosphorylated-Tau (Htau) were evaluated and CDNPs measured in ENS. CDNPs were abundant in erythrocytes, unmyelinated submucosal, perivascular and intramuscular nerve fibers, ganglionic neurons and vagus nerves and associated with organelle pathology. É‘Syn and Htau were present in 25/27 MC gastric,15/26 vagus and 18/27 gastric and 2/26 vagus samples respectively. We strongly suggest CDNPs are penetrating and damaging the GI barrier and reaching preganglionic parasympathetic fibers and the vagus nerve. This work highlights the potential role of CDNPs in the neuroenteric hyperphosphorylated É‘-Syn and tau pathology as seen in Parkinson and Alzheimer's diseases. Highly oxidative, ubiquitous CDNPs constitute a biologically plausible path into Parkinson's and Alzheimer's pathogenesis.
[Mh] Termos MeSH primário: Poluentes Atmosféricos/toxicidade
Intestino Delgado/efeitos dos fármacos
Nanopartículas/toxicidade
Nervo Vago/efeitos dos fármacos
Emissões de Veículos/toxicidade
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Biomarcadores/análise
Criança
Cidades
Cães
Feminino
Seres Humanos
Mucosa Intestinal/efeitos dos fármacos
Mucosa Intestinal/patologia
Mucosa Intestinal/ultraestrutura
Intestino Delgado/patologia
Intestino Delgado/ultraestrutura
Masculino
México
Microscopia Eletrônica de Transmissão
Fosforilação
RNA Mensageiro/análise
Junções Íntimas/efeitos dos fármacos
Junções Íntimas/patologia
Junções Íntimas/ultraestrutura
Nervo Vago/metabolismo
Nervo Vago/ultraestrutura
Adulto Jovem
alfa-Sinucleína/genética
alfa-Sinucleína/metabolismo
Proteínas tau/genética
Proteínas tau/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Biomarkers); 0 (RNA, Messenger); 0 (SNCA protein, human); 0 (Vehicle Emissions); 0 (alpha-Synuclein); 0 (tau Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170814
[St] Status:MEDLINE


  8 / 19283 MEDLINE  
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[PMID]:28778914
[Au] Autor:Tsai CY; Poon YY; Chen CH; Chan SHH
[Ad] Endereço:Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, Republic of China; and.
[Ti] Título:Anomalous baroreflex functionality inherent in floxed and Cre-Lox mice: an overlooked physiological phenotype.
[So] Source:Am J Physiol Heart Circ Physiol;313(4):H700-H707, 2017 Oct 01.
[Is] ISSN:1522-1539
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The last two decades have seen the emergence of Cre-Lox recombination as one of the most powerful and versatile technologies for cell-specific genetic engineering of mammalian cells. Understandably, the primary concerns in the practice of Cre-Lox recombination are whether the predicted genome has been correctly modified and the targeted phenotypes expressed. Rarely are the physiological conditions of the animals routinely examined because the general assumption is that they are normal. Based on corroborative results from radiotelemetric recording, power spectral analysis, and magnetic resonance imaging/diffusion tensor imaging in brain-derived neurotrophic factor-floxed mice, the present study revealed that this assumption requires amendment. We found that despite comparable blood pressure and heart rate with C57BL/6 or Cre mice under the conscious state, floxed and Cre-Lox mice exhibited diminished baroreflex-mediated sympathetic vasomotor tone and cardiac vagal baroreflex. We further found that the capacity and plasticity of baroreflex of these two strains of mice under isoflurane anesthesia were retarded, as reflected by reduced connectivity between the nucleus tractus solitarii and rostral ventrolateral medulla or nucleus ambiguus. The identification of anomalous baroreflex functionality inherent in floxed and Cre-Lox mice points to the importance of incorporating physiological phenotypes into studies that engage gene manipulations such as Cre-Lox recombination. We established that anomalous baroreflex functionality is inherent in floxed and Cre-Lox mice. These two mouse strains exhibited diminished baroreflex-mediated sympathetic vasomotor tone and cardiac vagal baroreflex under the conscious state, retarded capacity and plasticity of baroreflex under isoflurane anesthesia, and reduced connectivity between key nuclei in the baroreflex neural circuits.
[Mh] Termos MeSH primário: Barorreflexo/genética
Pressão Sanguínea/genética
Frequência Cardíaca/genética
Reflexo Anormal/genética
[Mh] Termos MeSH secundário: Anestésicos Inalatórios/farmacologia
Animais
Animais Geneticamente Modificados
Barorreflexo/efeitos dos fármacos
Barorreflexo/fisiologia
Pressão Sanguínea/fisiologia
Fator Neurotrófico Derivado do Encéfalo/genética
Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina/genética
Estado de Consciência
Imagem de Tensor de Difusão
Frequência Cardíaca/fisiologia
Integrases
Isoflurano/farmacologia
Imagem por Ressonância Magnética
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Vias Neurais
Fenótipo
Reflexo Anormal/efeitos dos fármacos
Reflexo Anormal/fisiologia
Núcleo Solitário/fisiopatologia
Nervo Vago/fisiopatologia
Sistema Vasomotor
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anesthetics, Inhalation); 0 (Brain-Derived Neurotrophic Factor); CYS9AKD70P (Isoflurane); EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2); EC 2.7.11.17 (Camk2a protein, mouse); EC 2.7.7.- (Cre recombinase); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170806
[St] Status:MEDLINE
[do] DOI:10.1152/ajpheart.00346.2017


  9 / 19283 MEDLINE  
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[PMID]:28705805
[Au] Autor:Diepenbroek C; Quinn D; Stephens R; Zollinger B; Anderson S; Pan A; de Lartigue G
[Ad] Endereço:The John B. Pierce Laboratory, New Haven, Connecticut.
[Ti] Título:Validation and characterization of a novel method for selective vagal deafferentation of the gut.
[So] Source:Am J Physiol Gastrointest Liver Physiol;313(4):G342-G352, 2017 Oct 01.
[Is] ISSN:1522-1547
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:There is a lack of tools that selectively target vagal afferent neurons (VAN) innervating the gut. We use saporin (SAP), a potent neurotoxin, conjugated to the gastronintestinal (GI) hormone cholecystokinin (CCK-SAP) injected into the nodose ganglia (NG) of male Wistar rats to specifically ablate GI-VAN. We report that CCK-SAP ablates a subpopulation of VAN in culture. In vivo, CCK-SAP injection into the NG reduces VAN innervating the mucosal and muscular layers of the stomach and small intestine but not the colon, while leaving vagal efferent neurons intact. CCK-SAP abolishes feeding-induced c-Fos in the NTS, as well as satiation by CCK or glucagon like peptide-1 (GLP-1). CCK-SAP in the NG of mice also abolishes CCK-induced satiation. Therefore, we provide multiple lines of evidence that injection of CCK-SAP in NG is a novel selective vagal deafferentation technique of the upper GI tract that works in multiple vertebrate models. This method provides improved tissue specificity and superior separation of afferent and efferent signaling compared with vagotomy, capsaicin, and subdiaphragmatic deafferentation. We develop a new method that allows targeted lesioning of vagal afferent neurons that innervate the upper GI tract while sparing vagal efferent neurons. This reliable approach provides superior tissue specificity and selectivity for vagal afferent over efferent targeting than traditional approaches. It can be used to address questions about the role of gut to brain signaling in physiological and pathophysiological conditions.
[Mh] Termos MeSH primário: Vias Aferentes/efeitos dos fármacos
Denervação Autônoma/métodos
Trato Gastrointestinal/efeitos dos fármacos
Bloqueio Nervoso/métodos
Proteínas Inativadoras de Ribossomos Tipo 1/administração & dosagem
Nervo Vago/efeitos dos fármacos
[Mh] Termos MeSH secundário: Vias Aferentes/fisiologia
Animais
Trato Gastrointestinal/fisiologia
Masculino
Neurotoxinas/administração & dosagem
Neurotoxinas/farmacologia
Ratos
Ratos Wistar
Resultado do Tratamento
Nervo Vago/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Neurotoxins); 0 (Ribosome Inactivating Proteins, Type 1); EC 3.2.2.22 (saporin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE
[do] DOI:10.1152/ajpgi.00095.2017


  10 / 19283 MEDLINE  
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[PMID]:28684460
[Au] Autor:Yuan PQ; Taché Y
[Ad] Endereço:CURE/Digestive Diseases Research Center, Center for Neurobiology of Stress and Resilience, Vatche and Tamar Manoukian Digestive Diseases Division, Department of Medicine and Brain Research Institute, University of California, Los Angeles, California; and pqyuan@mednet.ucla.edu.
[Ti] Título:Abdominal surgery induced gastric ileus and activation of M1-like macrophages in the gastric myenteric plexus: prevention by central vagal activation in rats.
[So] Source:Am J Physiol Gastrointest Liver Physiol;313(4):G320-G329, 2017 Oct 01.
[Is] ISSN:1522-1547
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Inflammation plays a role in abdominal surgery (AS)-induced intestinal ileus that is alleviated by electrical vagal stimulation. Intracisternal injection of RX-77368, the stable thyrotropin-releasing hormone agonist, activates dorsal motor nucleus neurons and gastric vagal efferent discharges. We investigated the gastric inflammation induced by AS and the modulation by intracisternal RX-77368 in rats. RX-77368 (50 ng/rat) or saline was injected followed, 1 h later, by laparotomy and small intestinal/cecal manipulation. The sham group had anesthesia alone. After 6 h, gastric emptying (GE) and the inflammation in gastric corpus were determined. AS inhibited GE by 72% vs. control and doubled the number of M1-like macrophage immunoreactive for major histocompatibility complex class II (MHCII; M1 marker) but not for cluster of differentiation 206 (CD206; M2 marker) (MHCII /CD206 ) while there was no change in M2-like macrophages (MHCII /CD206 ). AS increased mRNA levels of interleukin-1ß (IL-1ß) and tumor necrosis factor α (TNF-α) by 1.7- and 1.5-fold, respectively, in the gastric submucosa plus muscle layers and the infiltration of neutrophils labeled by myeloperoxidase by 9.5-fold in the muscularis externa. RX-77368 inhibited AS-related gastric changes while not altering these parameters in the sham group. There was a significant negative correlation between GE and IL-1ß ( = -0.46), TNF-α ( = -0.44), M1 macrophage ( = -0.82), and neutrophils ( = -0.91). The M2-like macrophages and IL-10 expression were unchanged by AS with intracisternal saline or RX-77368. These data indicate that AS activates gastric M1 macrophages and increases proinflammatory cytokines expression, which are prevented by central vagal activation and may contribute to the correlated dampening of postoperative gastric ileus. MHCII /CD206 (M1) and MHCII /CD206 (M2) constitute two distinct populations of macrophages that are in close apposition to the cholinergic neurons in the rat gastric myenteric plexus (MP). Abdominal surgery (6 h) activates M1 macrophage leading to inflammation in the gastric MP correlated with the delayed gastric emptying, which was abolished by central vagal stimulation via intracisternal injection of RX-77368. Vagal stimulation linked with the cephalic phase may have potential beneficial effects to curtail postoperative gastric ileus.
[Mh] Termos MeSH primário: Enterostomia/efeitos adversos
Motilidade Gastrointestinal/imunologia
Pseudo-Obstrução Intestinal/imunologia
Pseudo-Obstrução Intestinal/prevenção & controle
Ativação de Macrófagos/imunologia
Plexo Mientérico/fisiopatologia
Nervo Vago/fisiopatologia
[Mh] Termos MeSH secundário: Animais
Motilidade Gastrointestinal/efeitos dos fármacos
Pseudo-Obstrução Intestinal/etiologia
Ativação de Macrófagos/efeitos dos fármacos
Masculino
Plexo Mientérico/efeitos dos fármacos
Complexo Mioelétrico Migratório/efeitos dos fármacos
Complexo Mioelétrico Migratório/imunologia
Ácido Pirrolidonocarboxílico/administração & dosagem
Ácido Pirrolidonocarboxílico/análogos & derivados
Ratos
Ratos Sprague-Dawley
Hormônio Liberador de Tireotropina/administração & dosagem
Hormônio Liberador de Tireotropina/análogos & derivados
Resultado do Tratamento
Nervo Vago/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
5Y5F15120W (Thyrotropin-Releasing Hormone); 76820-40-1 (L-pyroglutamyl-L-histidyl-3,3-dimethylprolinamide); SZB83O1W42 (Pyrrolidonecarboxylic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE
[do] DOI:10.1152/ajpgi.00121.2017



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