Base de dados : MEDLINE
Pesquisa : A08.800.800.120.640 [Categoria DeCS]
Referências encontradas : 1622 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 163 ir para página                         

  1 / 1622 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28146061
[Au] Autor:Barton MJ; John JS; Clarke M; Wright A; Ekberg J
[Ad] Endereço:Menzies Health Institute Queensland, Griffith University, Nathan QLD 4111, Australia. m.barton@griffith.edu.au.
[Ti] Título:The Glia Response after Peripheral Nerve Injury: A Comparison between Schwann Cells and Olfactory Ensheathing Cells and Their Uses for Neural Regenerative Therapies.
[So] Source:Int J Mol Sci;18(2), 2017 Jan 29.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The peripheral nervous system (PNS) exhibits a much larger capacity for regeneration than the central nervous system (CNS). One reason for this difference is the difference in glial cell types between the two systems. PNS glia respond rapidly to nerve injury by clearing debris from the injury site, supplying essential growth factors and providing structural support; all of which enhances neuronal regeneration. Thus, transplantation of glial cells from the PNS is a very promising therapy for injuries to both the PNS and the CNS. There are two key types of PNS glia: olfactory ensheathing cells (OECs), which populate the olfactory nerve, and Schwann cells (SCs), which are present in the rest of the PNS. These two glial types share many similar morphological and functional characteristics but also exhibit key differences. The olfactory nerve is constantly turning over throughout life, which means OECs are continuously stimulating neural regeneration, whilst SCs only promote regeneration after direct injury to the PNS. This review presents a comparison between these two PNS systems in respect to normal physiology, developmental anatomy, glial functions and their responses to injury. A thorough understanding of the mechanisms and differences between the two systems is crucial for the development of future therapies using transplantation of peripheral glia to treat neural injuries and/or disease.
[Mh] Termos MeSH primário: Regeneração Nervosa
Neuroglia/metabolismo
Traumatismos dos Nervos Periféricos/metabolismo
Traumatismos dos Nervos Periféricos/patologia
[Mh] Termos MeSH secundário: Animais
Transplante de Células
Homeostase
Seres Humanos
Imunomodulação
Inflamação/imunologia
Inflamação/metabolismo
Inflamação/patologia
Neuroglia/imunologia
Bulbo Olfatório/citologia
Bulbo Olfatório/embriologia
Bulbo Olfatório/fisiologia
Nervo Olfatório/citologia
Nervo Olfatório/embriologia
Nervo Olfatório/fisiologia
Traumatismos dos Nervos Periféricos/imunologia
Traumatismos dos Nervos Periféricos/terapia
Células de Schwann/fisiologia
Células Receptoras Sensoriais/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170202
[St] Status:MEDLINE


  2 / 1622 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28112682
[Au] Autor:Van de Bittner GC; Riley MM; Cao L; Ehses J; Herrick SP; Ricq EL; Wey HY; O'Neill MJ; Ahmed Z; Murray TK; Smith JE; Wang C; Schroeder FA; Albers MW; Hooker JM
[Ti] Título:Nasal neuron PET imaging quantifies neuron generation and degeneration.
[So] Source:J Clin Invest;127(2):681-694, 2017 Feb 01.
[Is] ISSN:1558-8238
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Olfactory dysfunction is broadly associated with neurodevelopmental and neurodegenerative diseases and predicts increased mortality rates in healthy individuals. Conventional measurements of olfactory health assess odor processing pathways within the brain and provide a limited understanding of primary odor detection. Quantification of the olfactory sensory neurons (OSNs), which detect odors within the nasal cavity, would provide insight into the etiology of olfactory dysfunction associated with disease and mortality. Notably, OSNs are continually replenished by adult neurogenesis in mammals, including humans, so OSN measurements are primed to provide specialized insights into neurological disease. Here, we have evaluated a PET radiotracer, [11C]GV1-57, that specifically binds mature OSNs and quantifies the mature OSN population in vivo. [11C]GV1-57 monitored native OSN population dynamics in rodents, detecting OSN generation during postnatal development and aging-associated neurodegeneration. [11C]GV1-57 additionally measured rates of neuron regeneration after acute injury and early-stage OSN deficits in a rodent tauopathy model of neurodegenerative disease. Preliminary assessment in nonhuman primates suggested maintained uptake and saturable binding of [18F]GV1-57 in primate nasal epithelium, supporting its translational potential. Future applications for GV1-57 include monitoring additional diseases or conditions associated with olfactory dysregulation, including cognitive decline, as well as monitoring effects of neuroregenerative or neuroprotective therapeutics.
[Mh] Termos MeSH primário: Envelhecimento
Transtornos do Olfato/diagnóstico por imagem
Nervo Olfatório/diagnóstico por imagem
Condutos Olfatórios/diagnóstico por imagem
Tomografia por Emissão de Pósitrons/métodos
Tauopatias/diagnóstico por imagem
[Mh] Termos MeSH secundário: Animais
Masculino
Transtornos do Olfato/fisiopatologia
Nervo Olfatório/fisiopatologia
Condutos Olfatórios/fisiopatologia
Traçadores Radioativos
Ratos
Ratos Sprague-Dawley
Tauopatias/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Radioactive Tracers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE


  3 / 1622 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27864931
[Au] Autor:Kiyokage E; Kobayashi K; Toida K
[Ad] Endereço:Department of Anatomy, Kawasaki Medical School, Kurashiki, Okayama, 701-0192, Japan.
[Ti] Título:Spatial distribution of synapses on tyrosine hydroxylase-expressing juxtaglomerular cells in the mouse olfactory glomerulus.
[So] Source:J Comp Neurol;525(5):1059-1074, 2017 Apr 01.
[Is] ISSN:1096-9861
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Olfactory sensory axons converge in specific glomeruli where they form excitatory synapses onto dendrites of mitral/tufted (M/T) and juxtaglomerular (JG) cells, including periglomerular (PG), external tufted (ET), and superficial-short axon cells. JG cells consist of heterogeneous subpopulations with different neurochemical, physiological, and morphological properties. Among JG cells, previous electron microscopic (EM) studies have shown that the majority of synaptic inputs to tyrosine hydroxylase (TH)-immunoreactive neurons were asymmetrical synapses from olfactory nerve (ON) terminals. However, recent physiological results revealed that 70% of dopaminergic/γ-aminobutyric acid (GABA)ergic neurons received polysynaptic inputs via ET cells, whereas the remaining 30% received monosynaptic ON inputs. To understand the discrepancies between EM and physiological data, we used serial EM analysis combined with confocal laser scanning microscope images to examine the spatial distribution of synapses on dendrites using mice expressing enhanced green fluorescent protein under the control of the TH promoter. The majority of synaptic inputs to TH-expressing JG cells were from ON terminals, and they preferentially targeted distal dendrites from the soma. On the other hand, the numbers of non-ON inputs were fewer and targeted proximal dendrites. Furthermore, individual TH-expressing JG cells formed serial synapses, such as M/T→TH→another presumed M/T or ON→TH→presumed M/T, but not reciprocal synapses. Serotonergic fibers also associated with somatic regions of TH neurons, displaying non-ON profiles. Thus, fewer proximal non-ON synapses provide more effective inputs than large numbers of distal ON synapses and may occur on the physiologically characterized population of dopaminergic-GABAergic neurons (70%) that receive their most effective inputs indirectly via an ON→ET→TH circuit. J. Comp. Neurol. 525:1059-1074, 2017. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Neurônios/ultraestrutura
Bulbo Olfatório/ultraestrutura
Sinapses/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Imagem Tridimensional
Imuno-Histoquímica
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Microscopia Confocal
Microscopia Eletrônica de Transmissão
Nervo Olfatório/ultraestrutura
Tirosina 3-Mono-Oxigenase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE
[do] DOI:10.1002/cne.24147


  4 / 1622 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27864926
[Au] Autor:Matsuno T; Kiyokage E; Toida K
[Ad] Endereço:Department of Anatomy, Kawasaki Medical School, Okayama, Japan.
[Ti] Título:Synaptic distribution of individually labeled mitral cells in the external plexiform layer of the mouse olfactory bulb.
[So] Source:J Comp Neurol;525(7):1633-1648, 2017 May 01.
[Is] ISSN:1096-9861
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mitral cells are the major projection neurons of the olfactory bulb. They receive olfactory inputs, regulate information, and project their axons to the olfactory cortex. To understand output regulation of mitral cells better, we established a method to visualize individual projection neurons and quantitatively examined their synaptic distribution. Individual mitral cells were labeled by viral injection, reconstructed three dimensionally with light microscopy, and serial sectioned for electron microscopy. Synaptic distributions were analyzed in electron microscopically reconstructed cell bodies, two regions of secondary dendrites (near the somata and ∼200 µm from the somata), and primary dendrites. The ratio of presynaptic sites (60%) and reciprocal synapses (60% presynaptic and 80% postsynaptic sites) were similar in each region. Characteristically, primary dendrite synapses were distributed mainly within the inner half of the external plexiform layer (EPL). For comparison, tufted cells were also examined, and the synaptic distribution in two secondary dendrite regions, which corresponded with mitral cells, was analyzed. The results showed that the ratio of reciprocal synapses (80% presynaptic and 90% postsynaptic sites) was greater than in mitral cells. The distribution of symmetrical synapses was also analyzed with synaptic and neuronal markers, such as parvalbumin, vesicular gamma-aminobutyric acid transporter, and gephyrin. Parvalbumin-expressing neurons tended to form synapses on secondary dendrites near the somata and were more uniformly distributed on primary dendrites of mitral cells. These results indicate that local mitral cell synaptic circuits are formed in accordance with their functional roles and restricted to the inner half of the EPL. J. Comp. Neurol. 525:1633-1648, 2017. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Bulbo Olfatório/ultraestrutura
Nervo Olfatório/ultraestrutura
Sinapses/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Feminino
Imagem Tridimensional
Imuno-Histoquímica
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Microscopia Confocal
Microscopia Eletrônica de Transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE
[do] DOI:10.1002/cne.24148


  5 / 1622 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27506829
[Au] Autor:Tsutsumi S; Ono H; Yasumoto Y
[Ad] Endereço:Department of Neurological Surgery, Juntendo University Urayasu Hospital, 2-1-1 Tomioka, Urayasu, Chiba, 279-0021, Japan. shotaro@juntendo-urayasu.jp.
[Ti] Título:Visualization of the olfactory nerve using constructive interference in steady state magnetic resonance imaging.
[So] Source:Surg Radiol Anat;39(3):315-321, 2017 Mar.
[Is] ISSN:1279-8517
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The olfactory nerve (OlfN) is a small neural structure with inconsistent visualization on neuroimages. The aim of this study was to delineate the intracranial course of the OlfN using constructive interference in steady state magnetic resonance (MR) imaging. METHODS: A total of 168 patients were enrolled in this study. Following initial examinations with conventional MR sequences, constructive interference in steady-state sequence (CISS) was performed in coronal and axial sections. RESULTS: On coronal sections, the OlfN was entirely visualized in 90 % of patients on the right and 92 % on the left, coursing along the olfactory sulcus. Complete visualization of the OlfN occurred in 100 % of patients on serial axial images. The OlfN was classified into four portions based on the topographical differences and surrounding structures. The olfactory fossa exhibited considerable variability at the midlevel of the olfactory bulb on coronal images. Characteristic appearance of the OlfN with respect to age range or gender was not observed. CONCLUSIONS: The OlfN follows a highly consistent course along the olfactory sulcus. Thin-sliced, CISS sequences are useful for consistent visualization of the OlfN.
[Mh] Termos MeSH primário: Bulbo Olfatório/diagnóstico por imagem
Nervo Olfatório/diagnóstico por imagem
[Mh] Termos MeSH secundário: Adolescente
Adulto
Fatores Etários
Idoso
Idoso de 80 Anos ou mais
Cadáver
Criança
Dissecação
Feminino
Seres Humanos
Imagem por Ressonância Magnética/métodos
Masculino
Meia-Idade
Fatores Sexuais
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160811
[St] Status:MEDLINE
[do] DOI:10.1007/s00276-016-1731-9


  6 / 1622 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27297522
[Au] Autor:Al Salihi MO; Kobayashi M; Tamari K; Miyamura T; Takeuchi K
[Ad] Endereço:Department of Otorhinolaryngology-Head and Neck Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
[Ti] Título:Tumor necrosis factor-α antagonist suppresses local inflammatory reaction and facilitates olfactory nerve recovery following injury.
[So] Source:Auris Nasus Larynx;44(1):70-78, 2017 Feb.
[Is] ISSN:1879-1476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Olfactory dysfunction is a common finding in head trauma due to injury to the olfactory nerve. We previously reported that anti-inflammatory treatment with steroids improves recovery outcome in olfactory nerve injury models. Clinically, however, steroid administration is not recommended in the acute phase of head injury cases because of concerns regarding its side effects. Tumor necrosis factor (TNF-α) is known to play a key role in inflammatory response to injury. The present study examines if the inhibition of TNF-α can facilitate functional recovery in the olfactory system following injury. MATERIALS AND METHODS: Olfactory nerve transection (NTx) was performed in olfactory marker protein (OMP-tau-lacZ) mice to establish injury models. We measured TNF-α gene expression in the olfactory bulb using semi-quantitative and real time polymerase chain reaction (PCR) assays and found that they increase within hours after NTx injury. A TNF-α antagonist (etanercept) was intraperitoneally injected immediately after the NTx and histological assessment of recovery within the olfactory bulb was performed at 5-70 days. X-gal staining labeled OMP in the degenerating and regenerating olfactory nerve fibers, and immunohistochemical staining detected the presence of reactive astrocytes and macrophages/microglia. RESULTS: Etanercept-injected mice showed significantly smaller areas of injury-associated tissue, fewer astrocytes and macrophages/microglia, and an increase in regenerating nerve fibers. Olfactory function assessments using both an olfactory avoidance behavioral test and evoked potential recordings showed improved functional recovery in etanercept-injected animals. CONCLUSION: These findings suggest that inhibition of TNF-α could provide a new therapeutic strategy for the treatment of olfactory dysfunction following head injuries.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/farmacologia
Etanercepte/farmacologia
Inflamação
Bulbo Olfatório/efeitos dos fármacos
Traumatismos do Nervo Olfatório
Nervo Olfatório/efeitos dos fármacos
Recuperação de Função Fisiológica/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Astrócitos/efeitos dos fármacos
Modelos Animais de Doenças
Feminino
Macrófagos/efeitos dos fármacos
Masculino
Camundongos
Camundongos Transgênicos
Microglia/efeitos dos fármacos
Bulbo Olfatório/metabolismo
Proteína de Marcador Olfatório/genética
Nervo Olfatório/imunologia
Nervo Olfatório/patologia
Reação em Cadeia da Polimerase em Tempo Real
Fator de Necrose Tumoral alfa/antagonistas & inibidores
Fator de Necrose Tumoral alfa/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Olfactory Marker Protein); 0 (Omp protein, mouse); 0 (Tumor Necrosis Factor-alpha); OP401G7OJC (Etanercept)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170723
[Lr] Data última revisão:
170723
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160615
[St] Status:MEDLINE


  7 / 1622 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27498858
[Au] Autor:Musashe DT; Purice MD; Speese SD; Doherty J; Logan MA
[Ad] Endereço:Department of Neurology, Jungers Center for Neurosciences Research, Oregon Health and Science University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97239, USA.
[Ti] Título:Insulin-like Signaling Promotes Glial Phagocytic Clearance of Degenerating Axons through Regulation of Draper.
[So] Source:Cell Rep;16(7):1838-50, 2016 08 16.
[Is] ISSN:2211-1247
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Neuronal injury triggers robust responses from glial cells, including altered gene expression and enhanced phagocytic activity to ensure prompt removal of damaged neurons. The molecular underpinnings of glial responses to trauma remain unclear. Here, we find that the evolutionarily conserved insulin-like signaling (ILS) pathway promotes glial phagocytic clearance of degenerating axons in adult Drosophila. We find that the insulin-like receptor (InR) and downstream effector Akt1 are acutely activated in local ensheathing glia after axotomy and are required for proper clearance of axonal debris. InR/Akt1 activity, it is also essential for injury-induced activation of STAT92E and its transcriptional target draper, which encodes a conserved receptor essential for glial engulfment of degenerating axons. Increasing Draper levels in adult glia partially rescues delayed clearance of severed axons in glial InR-inhibited flies. We propose that ILS functions as a key post-injury communication relay to activate glial responses, including phagocytic activity.
[Mh] Termos MeSH primário: Proteínas de Drosophila/genética
Drosophila melanogaster/genética
Insulina/metabolismo
Proteínas de Membrana/genética
Neuroglia/metabolismo
Neurônios/metabolismo
Receptores Proteína Tirosina Quinases/genética
[Mh] Termos MeSH secundário: Animais
Axotomia
Comunicação Celular
Proteínas de Drosophila/deficiência
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/metabolismo
Regulação da Expressão Gênica
Proteínas de Membrana/metabolismo
Neuroglia/citologia
Neurônios/patologia
Nervo Olfatório/cirurgia
Fagocitose
Proteínas Proto-Oncogênicas c-akt/genética
Proteínas Proto-Oncogênicas c-akt/metabolismo
Receptores Proteína Tirosina Quinases/deficiência
Fatores de Transcrição STAT/genética
Fatores de Transcrição STAT/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Draper protein, Drosophila); 0 (Drosophila Proteins); 0 (Insulin); 0 (Membrane Proteins); 0 (STAT Transcription Factors); 0 (Stat92E protein, Drosophila); EC 2.7.10.1 (InR protein, Drosophila); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases); EC 2.7.11.1 (Akt1 protein, Drosophila); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160809
[St] Status:MEDLINE


  8 / 1622 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27463556
[Au] Autor:Jenkins EK; Lee-Fowler TM; Angle TC; Behrend EN; Moore GE
[Ti] Título:Effects of oral administration of metronidazole and doxycycline on olfactory capabilities of explosives detection dogs.
[So] Source:Am J Vet Res;77(8):906-12, 2016 Aug.
[Is] ISSN:1943-5681
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE To determine effects of oral administration of metronidazole or doxycycline on olfactory function in explosives detection (ED) dogs. ANIMALS 18 ED dogs. PROCEDURES Metronidazole was administered (25 mg/kg, PO, q 12 h for 10 days); the day prior to drug administration was designated day 0. Odor detection threshold was measured with a standard scent wheel and 3 explosives (ammonium nitrate, trinitrotoluene, and smokeless powder; weight, 1 to 500 mg) on days 0, 5, and 10. Lowest repeatable weight detected was recorded as the detection threshold. There was a 10-day washout period, and doxycycline was administered (5 mg/kg, PO, q 12 h for 10 days) and the testing protocol repeated. Degradation changes in the detection threshold for dogs were assessed. RESULTS Metronidazole administration resulted in degradation of the detection threshold for 2 of 3 explosives (ammonium nitrate and trinitrotoluene). Nine of 18 dogs had a degradation of performance in response to 1 or more explosives (5 dogs had degradation on day 5 or 10 and 4 dogs had degradation on both days 5 and 10). There was no significant degradation during doxycycline administration. CONCLUSIONS AND CLINICAL RELEVANCE Degradation in the ability to detect odors of explosives during metronidazole administration at 25 mg/kg, PO, every 12 hours, indicated a potential risk for use of this drug in ED dogs. Additional studies will be needed to determine whether lower doses would have the same effect. Doxycycline administered at the tested dose appeared to be safe for use in ED dogs.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Doxiciclina/farmacologia
Metronidazol/farmacologia
Nervo Olfatório/efeitos dos fármacos
[Mh] Termos MeSH secundário: Administração Oral
Animais
Anti-Infecciosos/administração & dosagem
Cães
Doxiciclina/administração & dosagem
Substâncias Explosivas/química
Feminino
Masculino
Metronidazol/administração & dosagem
Odorantes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Explosive Agents); 140QMO216E (Metronidazole); N12000U13O (Doxycycline)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170123
[Lr] Data última revisão:
170123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE
[do] DOI:10.2460/ajvr.77.8.906


  9 / 1622 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27448390
[Au] Autor:Siegers JY; van den Brand JM; Leijten LM; van de Bildt MM; van Run PR; van Amerongen G; Stittelaar KJ; Koopmans MP; Osterhaus AD; Kuiken T; van Riel D
[Ad] Endereço:Department of Viroscience, Erasmus MC.
[Ti] Título:Vaccination Is More Effective Than Prophylactic Oseltamivir in Preventing CNS Invasion by H5N1 Virus via the Olfactory Nerve.
[So] Source:J Infect Dis;214(4):516-24, 2016 Aug 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Influenza A viruses can replicate in the olfactory mucosa and subsequently use the olfactory nerve to enter the central nervous system (CNS). It is currently unknown whether intervention strategies are able to reduce or prevent influenza virus replication within the olfactory mucosa and subsequent spread to the CNS. Therefore, we tested the efficacy of homologous vaccination and prophylactic oseltamivir to prevent H5N1 virus CNS invasion via the olfactory nerve in our ferret model. METHODS: Ferrets were vaccinated intramuscularly or received oseltamivir (5 mg/kg twice daily) prophylactically before intranasal inoculation of highly pathogenic H5N1 virus (A/Indonesia/05/2005) and were examined using virology and pathology. RESULTS: Homologous vaccination reduced H5N1 virus replication in the olfactory mucosa and prevented subsequent virus spread to the CNS. However, prophylactic oseltamivir did not prevent H5N1 virus replication in the olfactory mucosa sufficiently, resulting in CNS invasion via the olfactory nerve causing a severe meningoencephalitis. CONCLUSIONS: Within our ferret model, vaccination is more effective than prophylactic oseltamivir in preventing CNS invasion by H5N1 virus via the olfactory nerve. This study highlights the importance of including the olfactory mucosa, olfactory nerve, and CNS tissues in future vaccine and antiviral studies, especially for viruses with a known neurotropic potential.
[Mh] Termos MeSH primário: Antivirais/administração & dosagem
Vírus da Influenza A Subtipo H5N1/isolamento & purificação
Vacinas contra Influenza/administração & dosagem
Meningoencefalite/prevenção & controle
Infecções por Orthomyxoviridae/complicações
Oseltamivir/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Quimioprevenção/métodos
Modelos Animais de Doenças
Feminino
Furões
Vírus da Influenza A Subtipo H5N1/efeitos dos fármacos
Vírus da Influenza A Subtipo H5N1/imunologia
Injeções Intramusculares
Nervo Olfatório/virologia
Infecções por Orthomyxoviridae/patologia
Infecções por Orthomyxoviridae/virologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Influenza Vaccines); 20O93L6F9H (Oseltamivir)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jiw123


  10 / 1622 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27377344
[Au] Autor:Vaaga CE; Westbrook GL
[Ad] Endereço:Vollum Institute.
[Ti] Título:Parallel processing of afferent olfactory sensory information.
[So] Source:J Physiol;594(22):6715-6732, 2016 Nov 15.
[Is] ISSN:1469-7793
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:KEY POINTS: The functional synaptic connectivity between olfactory receptor neurons and principal cells within the olfactory bulb is not well understood. One view suggests that mitral cells, the primary output neuron of the olfactory bulb, are solely activated by feedforward excitation. Using focal, single glomerular stimulation, we demonstrate that mitral cells receive direct, monosynaptic input from olfactory receptor neurons. Compared to external tufted cells, mitral cells have a prolonged afferent-evoked EPSC, which serves to amplify the synaptic input. The properties of presynaptic glutamate release from olfactory receptor neurons are similar between mitral and external tufted cells. Our data suggest that afferent input enters the olfactory bulb in a parallel fashion. ABSTRACT: Primary olfactory receptor neurons terminate in anatomically and functionally discrete cortical modules known as olfactory bulb glomeruli. The synaptic connectivity and postsynaptic responses of mitral and external tufted cells within the glomerulus may involve both direct and indirect components. For example, it has been suggested that sensory input to mitral cells is indirect through feedforward excitation from external tufted cells. We also observed feedforward excitation of mitral cells with weak stimulation of the olfactory nerve layer; however, focal stimulation of an axon bundle entering an individual glomerulus revealed that mitral cells receive monosynaptic afferent inputs. Although external tufted cells had a 4.1-fold larger peak EPSC amplitude, integration of the evoked currents showed that the synaptic charge was 5-fold larger in mitral cells, reflecting the prolonged response in mitral cells. Presynaptic afferents onto mitral and external tufted cells had similar quantal amplitude and release probability, suggesting that the larger peak EPSC in external tufted cells was the result of more synaptic contacts. The results of the present study indicate that the monosynaptic afferent input to mitral cells depends on the strength of odorant stimulation. The enhanced spiking that we observed in response to brief afferent input provides a mechanism for amplifying sensory information and contrasts with the transient response in external tufted cells. These parallel input paths may have discrete functions in processing olfactory sensory input.
[Mh] Termos MeSH primário: Neurônios Aferentes/fisiologia
Bulbo Olfatório/fisiologia
Neurônios Receptores Olfatórios/fisiologia
[Mh] Termos MeSH secundário: Animais
Estimulação Elétrica/métodos
Potenciais Pós-Sinápticos Excitadores/fisiologia
Feminino
Masculino
Potenciais da Membrana/fisiologia
Camundongos
Camundongos Endogâmicos C57BL
Nervo Olfatório/fisiologia
Olfato/fisiologia
Transmissão Sináptica/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160706
[St] Status:MEDLINE
[do] DOI:10.1113/JP272755



página 1 de 163 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde