Base de dados : MEDLINE
Pesquisa : A08.850.800 [Categoria DeCS]
Referências encontradas : 5596 [refinar]
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[PMID]:28716844
[Au] Autor:Cantaut-Belarif Y; Antri M; Pizzarelli R; Colasse S; Vaccari I; Soares S; Renner M; Dallel R; Triller A; Bessis A
[Ad] Endereço:École Normale Supérieure, Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique, Paris Sciences et Lettres Research University, Paris, France.
[Ti] Título:Microglia control the glycinergic but not the GABAergic synapses via prostaglandin E2 in the spinal cord.
[So] Source:J Cell Biol;216(9):2979-2989, 2017 Sep 04.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microglia control excitatory synapses, but their role in inhibitory neurotransmission has been less well characterized. Herein, we show that microglia control the strength of glycinergic but not GABAergic synapses via modulation of the diffusion dynamics and synaptic trapping of glycine (GlyR) but not GABA receptors. We further demonstrate that microglia regulate the activity-dependent plasticity of glycinergic synapses by tuning the GlyR diffusion trap. This microglia-synapse cross talk requires production of prostaglandin E2 by microglia, leading to the activation of neuronal EP2 receptors and cyclic adenosine monophosphate-dependent protein kinase. Thus, we now provide a link between microglial activation and synaptic dysfunctions, which are common early features of many brain diseases.
[Mh] Termos MeSH primário: Dinoprostona/metabolismo
Sinapses Elétricas/metabolismo
Glicina/metabolismo
Microglia/metabolismo
Inibição Neural
Medula Espinal/metabolismo
Transmissão Sináptica
Ácido gama-Aminobutírico/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Transporte/genética
Proteínas de Transporte/metabolismo
Células Cultivadas
Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
Difusão
Feminino
Masculino
Potenciais da Membrana
Proteínas de Membrana/genética
Proteínas de Membrana/metabolismo
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Transporte Proteico
Receptores de GABA-A/metabolismo
Receptores da Glicina/metabolismo
Receptores de Prostaglandina E Subtipo EP2/metabolismo
Membranas Sinápticas/metabolismo
Fatores de Tempo
Técnicas de Cultura de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Membrane Proteins); 0 (Ptger2 protein, mouse); 0 (Receptors, GABA-A); 0 (Receptors, Glycine); 0 (Receptors, Prostaglandin E, EP2 Subtype); 0 (gephyrin); 56-12-2 (gamma-Aminobutyric Acid); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); K7Q1JQR04M (Dinoprostone); TE7660XO1C (Glycine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201607048


  2 / 5596 MEDLINE  
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[PMID]:28630145
[Au] Autor:Goo MS; Sancho L; Slepak N; Boassa D; Deerinck TJ; Ellisman MH; Bloodgood BL; Patrick GN
[Ad] Endereço:Section of Neurobiology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA.
[Ti] Título:Activity-dependent trafficking of lysosomes in dendrites and dendritic spines.
[So] Source:J Cell Biol;216(8):2499-2513, 2017 Aug 07.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In neurons, lysosomes, which degrade membrane and cytoplasmic components, are thought to primarily reside in somatic and axonal compartments, but there is little understanding of their distribution and function in dendrites. Here, we used conventional and two-photon imaging and electron microscopy to show that lysosomes traffic bidirectionally in dendrites and are present in dendritic spines. We find that lysosome inhibition alters their mobility and also decreases dendritic spine number. Furthermore, perturbing microtubule and actin cytoskeletal dynamics has an inverse relationship on the distribution and motility of lysosomes in dendrites. We also find trafficking of lysosomes is correlated with synaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptors. Strikingly, lysosomes traffic to dendritic spines in an activity-dependent manner and can be recruited to individual spines in response to local activation. These data indicate the position of lysosomes is regulated by synaptic activity and thus plays an instructive role in the turnover of synaptic membrane proteins.
[Mh] Termos MeSH primário: Dendritos/metabolismo
Espinhas Dendríticas/metabolismo
Hipocampo/metabolismo
Lisossomos/metabolismo
Proteínas de Membrana/metabolismo
Proteínas do Tecido Nervoso/metabolismo
Membranas Sinápticas/metabolismo
[Mh] Termos MeSH secundário: Citoesqueleto de Actina/metabolismo
Animais
Animais Recém-Nascidos
Dendritos/ultraestrutura
Espinhas Dendríticas/ultraestrutura
Feminino
Células HEK293
Hipocampo/ultraestrutura
Seres Humanos
Lisossomos/ultraestrutura
Masculino
Microscopia Eletrônica
Microscopia de Fluorescência por Excitação Multifotônica
Microscopia de Vídeo
Microtúbulos/metabolismo
Desnaturação Proteica
Ratos Sprague-Dawley
Receptores de Glutamato/metabolismo
Receptores de N-Metil-D-Aspartato/metabolismo
Fatores de Tempo
Imagem com Lapso de Tempo
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Nm] Nome de substância:
0 (Membrane Proteins); 0 (Nerve Tissue Proteins); 0 (Receptors, Glutamate); 0 (Receptors, N-Methyl-D-Aspartate); 0 (alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid subtype glutamate receptor, human)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201704068


  3 / 5596 MEDLINE  
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[PMID]:28474392
[Au] Autor:Fujii S; Tanaka H; Hirano T
[Ad] Endereço:Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
[Ti] Título:Detection and characterization of individual endocytosis of AMPA-type glutamate receptor around postsynaptic membrane.
[So] Source:Genes Cells;22(6):583-590, 2017 Jun.
[Is] ISSN:1365-2443
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Synaptic plasticity such as long-term depression (LTD) has been regarded as a cellular mechanism of learning and memory. LTD is expressed by the decrease in number of postsynaptic AMPA-type receptor (AMPAR) at glutamatergic synapses. Although endocytosis is known to play an essential role in the decrease in AMPAR on postsynaptic membrane, the difficulty to detect individual endocytic events hampered clarification of AMPAR dynamics around synapses. Previously, we developed a method to induce formation of postsynaptic-like membrane (PSLM) on the glass surface and observed pHluorin-tagged AMPAR around PSLM with total internal reflection fluorescence microscopy. By this method, individual exocytosis of AMPAR-pHluorin was recorded in both PSLM and non-PSLM. In other studies, endocytic vesicles containing pHluorin-tagged receptors were visualized by changing extracellular pH. Here, we have combined PSLM formation method and rapid pH change method, and detected individual endocytic events of AMPAR around PSLM with high spatial and temporal resolutions. Endocytic events of AMPAR were characterized by comparison with those of transferrin receptor. Constitutive endocytosis of AMPAR was not dependent on clathrin and dynamin in contrast to that of transferrin receptor. However, AMPAR endocytosis triggered by LTD-inducing stimulation was clathrin- and dynamin-dependent.
[Mh] Termos MeSH primário: Endocitose
Hipocampo/metabolismo
Receptores de AMPA/metabolismo
Receptores da Transferrina/metabolismo
Sinapses/metabolismo
Membranas Sinápticas/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Clatrina/metabolismo
Dinaminas/metabolismo
Endocitose/efeitos dos fármacos
Agonistas de Aminoácidos Excitatórios/farmacologia
Proteínas de Fluorescência Verde/análise
Hipocampo/efeitos dos fármacos
Hipocampo/embriologia
N-Metilaspartato/farmacologia
Transporte Proteico
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Clathrin); 0 (Excitatory Amino Acid Agonists); 0 (Receptors, AMPA); 0 (Receptors, Transferrin); 0 (glutamate receptor ionotropic, AMPA 1); 147336-22-9 (Green Fluorescent Proteins); 6384-92-5 (N-Methylaspartate); EC 3.6.5.5 (Dynamins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1111/gtc.12493


  4 / 5596 MEDLINE  
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[PMID]:28362857
[Au] Autor:Burch A; Tao-Cheng JH; Dosemeci A
[Ad] Endereço:Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America.
[Ti] Título:A novel synaptic junction preparation for the identification and characterization of cleft proteins.
[So] Source:PLoS One;12(3):e0174895, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Identification of synaptic cleft components has been hampered by the lack of a suitable preparation enriched in synaptic junctions devoid of adjoining peripheral membranes. Prior strategies for the isolation of synaptic junctions, relying on detergents for the removal of peripheral membranes, resulted in substantial loss of membranes lining the cleft. Here, a novel, detergent-free method is described for the preparation of a synaptic junction (SJ) fraction, using phospholipase A2. Limited digestion of synaptic plasma membrane (SPM) fraction with phospholipase A2 followed by centrifugation over a sucrose cushion results in selective removal of membranes peripheral to the cleft while junctional membranes remain relatively intact as observed by electron microscopy. Enrichment in synaptic junctional structures and loss of membranes peripheral to the junctional area are further verified by demonstrating enrichment in PSD-95 and loss in mGluR5, respectively. The SJ fraction is enriched in neuroligins and neurexins, in agreement with immuno-electron microscopy data showing their selective localization to the junctional area. Among additional cell adhesion molecules tested, N-cadherin and specific isoforms of the SynCAM and SALM families also show marked enrichment in the SJ fraction, suggesting preferential localization at the synaptic cleft while others show little enrichment or decrease, suggesting that they are not restricted to or concentrated at the synaptic cleft. Treatment of the SJ fraction with glycosidases results in electrophoretic mobility shifts of all cell adhesion molecules tested, indicating glycosylation at the synaptic cleft. Biochemical and ultrastructural data presented indicate that the novel synaptic junction preparation can be used as a predictive tool for the identification and characterization of the components of the synaptic cleft.
[Mh] Termos MeSH primário: Sinapses/metabolismo
[Mh] Termos MeSH secundário: Animais
Western Blotting
Adesão Celular/fisiologia
Proteína 4 Homóloga a Disks-Large
Peptídeos e Proteínas de Sinalização Intracelular/metabolismo
Proteínas de Membrana/metabolismo
Microscopia Imunoeletrônica
Fosfolipases A2/metabolismo
Ratos
Ratos Sprague-Dawley
Receptor de Glutamato Metabotrópico 5/metabolismo
Sinapses/ultraestrutura
Membranas Sinápticas/metabolismo
Membranas Sinápticas/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disks Large Homolog 4 Protein); 0 (Dlg4 protein, rat); 0 (Intracellular Signaling Peptides and Proteins); 0 (Membrane Proteins); 0 (Receptor, Metabotropic Glutamate 5); EC 3.1.1.4 (Phospholipases A2)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174895


  5 / 5596 MEDLINE  
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[PMID]:28324066
[Au] Autor:Bailey DJ; Makeyeva YV; Paitel ER; Pedersen AL; Hon AT; Gunderson JA; Saldanha CJ
[Ad] Endereço:Biology, St. Norbert College, De Pere, Wisconsin .
[Ti] Título:Hippocampal Aromatization Modulates Spatial Memory and Characteristics of the Synaptic Membrane in the Male Zebra Finch.
[So] Source:Endocrinology;158(4):852-859, 2017 04 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The estrogen-synthesizing enzyme aromatase is abundant at the synapse in the zebra finch hippocampus (HP), and its inhibition impairs spatial memory function. To more fully test the role of local estradiol (E2) synthesis in memory, the HP of adult male zebra finches was exposed to either control pellets or those containing the aromatase inhibitor 1,4,6-androstatriene-3,17-dione (ATD), ATD and E2, ATD and the G protein-coupled estrogen receptor (GPER) agonist G1, or the antagonist G15 alone. Birds were tested for spatial memory acquisition and performance, and HP levels of the postsynaptic protein PSD95 were measured. ATD-treated birds took longer to reach criterion than control birds, whereas acquisition in ATD+E2 and ATD+G1 birds was indistinguishable from control and ATD treatments. Interestingly, all G15 birds failed to acquire the task. Following a retention interval, ATD birds took the longest to reach the (formerly) baited cup and made the most mistakes. ATD+E2 animals displayed the lowest retention latencies and made fewer mistakes than ATD-treated birds, and ATD+G1 birds did not significantly differ from controls in retention latencies. The amount of PSD95 in the HP was lowest in ATD-treated animals compared with birds with silicone-only-implanted craniotomies, ATD+E2, and ATD+G1 birds, who did not differ in this expression. Thus, spatial memory acquisition and performance appear aromatase and E2 dependent, an effect more reliably revealed after consolidation and/or recall compared to acquisition. E2 may exert this effect via GPERs, resulting in an increase in PSD95 levels that may modify receptor activity or intracellular signaling pathways to increase synaptic strength.
[Mh] Termos MeSH primário: Inibidores da Aromatase/farmacologia
Aromatase/metabolismo
Hipocampo/efeitos dos fármacos
Memória Espacial/efeitos dos fármacos
Membranas Sinápticas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Androstatrienos/farmacologia
Animais
Benzodioxóis/farmacologia
Estradiol/farmacologia
Estrogênios/farmacologia
Tentilhões
Hipocampo/metabolismo
Masculino
Quinolinas/farmacologia
Membranas Sinápticas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (4-(6-bromo-1,3-benzodioxol-5-yl)-3a,4,5,9b-3H-cyclopenta(c)quinoline); 0 (Androstatrienes); 0 (Aromatase Inhibitors); 0 (Benzodioxoles); 0 (Estrogens); 0 (Quinolines); 217A6T1V8N (androsta-1,4,6-triene-3,17-dione); 4TI98Z838E (Estradiol); EC 1.14.14.1 (Aromatase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1210/en.2016-1692


  6 / 5596 MEDLINE  
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[PMID]:28212274
[Au] Autor:Shirai Y; Li W; Suzuki T
[Ad] Endereço:Department of Neuroplasticity, Institute of Pathogenesis and Disease Prevention, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan. yoshirai@shinshu-u.ac.jp.
[Ti] Título:Role of Splice Variants of Gtf2i, a Transcription Factor Localizing at Postsynaptic Sites, and Its Relation to Neuropsychiatric Diseases.
[So] Source:Int J Mol Sci;18(2), 2017 Feb 15.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:We previously reported that various mRNAs were associated with postsynaptic density (PSD) purified from rat forebrain. Among the thousands of PSD-associated mRNAs, we highlight the biology of the general transcription factor II-I ( ) mRNA, focusing on the significance of its versatile splicing for targeting its own mRNA into dendrites, regulation of translation, and the effects of expression level as well as its relationship with neuropsychiatric disorders.
[Mh] Termos MeSH primário: Processamento Alternativo
Transtornos Mentais/genética
Transtornos Mentais/metabolismo
Doenças do Sistema Nervoso/genética
Doenças do Sistema Nervoso/metabolismo
Membranas Sinápticas/metabolismo
Fatores de Transcrição TFII/genética
Fatores de Transcrição TFII/metabolismo
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Animais
Variações do Número de Cópias de DNA
Regulação da Expressão Gênica
Estudos de Associação Genética
Predisposição Genética para Doença
Seres Humanos
Biossíntese de Proteínas
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (RNA, Messenger); 0 (Transcription Factors, TFII)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE


  7 / 5596 MEDLINE  
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[PMID]:28132827
[Au] Autor:Sinnen BL; Bowen AB; Forte JS; Hiester BG; Crosby KC; Gibson ES; Dell'Acqua ML; Kennedy MJ
[Ad] Endereço:Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
[Ti] Título:Optogenetic Control of Synaptic Composition and Function.
[So] Source:Neuron;93(3):646-660.e5, 2017 Feb 08.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The molecular composition of the postsynaptic membrane is sculpted by synaptic activity. During synaptic plasticity at excitatory synapses, numerous structural, signaling, and receptor molecules concentrate at the postsynaptic density (PSD) to regulate synaptic strength. We developed an approach that uses light to tune the abundance of specific molecules in the PSD. We used this approach to investigate the relationship between the number of AMPA-type glutamate receptors in the PSD and synaptic strength. Surprisingly, adding more AMPA receptors to excitatory contacts had little effect on synaptic strength. Instead, we observed increased excitatory input through the apparent addition of new functional sites. Our data support a model where adding AMPA receptors is sufficient to activate synapses that had few receptors to begin with, but that additional remodeling events are required to strengthen established synapses. More broadly, this approach introduces the precise spatiotemporal control of optogenetics to the molecular control of synaptic function.
[Mh] Termos MeSH primário: Plasticidade Neuronal/genética
Neurônios/metabolismo
Optogenética/métodos
Densidade Pós-Sináptica/metabolismo
Receptores de AMPA/genética
Sinapses/metabolismo
Membranas Sinápticas/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Arabidopsis/genética
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina/metabolismo
Criptocromos/genética
Hipocampo/citologia
Potenciação de Longa Duração
Técnicas de Patch-Clamp
Ratos
Ratos Sprague-Dawley
Sinapses/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Basic Helix-Loop-Helix Transcription Factors); 0 (CIB1 protein, Arabidopsis); 0 (CRY2 protein, Arabidopsis); 0 (Cryptochromes); 0 (Receptors, AMPA); 0 (glutamate receptor ionotropic, AMPA 1); 0 (glutamate receptor ionotropic, AMPA 2); EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171014
[Lr] Data última revisão:
171014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170131
[St] Status:MEDLINE


  8 / 5596 MEDLINE  
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[PMID]:27941024
[Au] Autor:Papadopoulos T; Rhee HJ; Subramanian D; Paraskevopoulou F; Mueller R; Schultz C; Brose N; Rhee JS; Betz H
[Ad] Endereço:From the Department of Molecular Biology, Center of Biochemistry and Molecular Cell Biology, Universitätsmedizin Göttingen, Humboldtallee 23, 37073 Göttingen, Germany, theofilos.papadopoulos@med.uni-goettingen.de.
[Ti] Título:Endosomal Phosphatidylinositol 3-Phosphate Promotes Gephyrin Clustering and GABAergic Neurotransmission at Inhibitory Postsynapses.
[So] Source:J Biol Chem;292(4):1160-1177, 2017 Jan 27.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The formation of neuronal synapses and the dynamic regulation of their efficacy depend on the proper assembly of the postsynaptic neurotransmitter receptor apparatus. Receptor recruitment to inhibitory GABAergic postsynapses requires the scaffold protein gephyrin and the guanine nucleotide exchange factor collybistin (Cb). In vitro, the pleckstrin homology domain of Cb binds phosphoinositides, specifically phosphatidylinositol 3-phosphate (PI3P). However, whether PI3P is required for inhibitory postsynapse formation is currently unknown. Here, we investigated the role of PI3P at developing GABAergic postsynapses by using a membrane-permeant PI3P derivative, time-lapse confocal imaging, electrophysiology, as well as knockdown and overexpression of PI3P-metabolizing enzymes. Our results provide the first in cellula evidence that PI3P located at early/sorting endosomes regulates the postsynaptic clustering of gephyrin and GABA receptors and the strength of inhibitory, but not excitatory, postsynapses in cultured hippocampal neurons. In human embryonic kidney 293 cells, stimulation of gephyrin cluster formation by PI3P depends on Cb. We therefore conclude that the endosomal pool of PI3P, generated by the class III phosphatidylinositol 3-kinase, is important for the Cb-mediated recruitment of gephyrin and GABA receptors to developing inhibitory postsynapses and thus the formation of postsynaptic membrane specializations.
[Mh] Termos MeSH primário: Proteínas de Transporte/metabolismo
Endossomos/metabolismo
Neurônios GABAérgicos/metabolismo
Proteínas de Membrana/metabolismo
Fosfatos de Fosfatidilinositol/metabolismo
Membranas Sinápticas/metabolismo
Potenciais Sinápticos/fisiologia
[Mh] Termos MeSH secundário: Animais
Neurônios GABAérgicos/citologia
Seres Humanos
Fosfatidilinositol 3-Quinases/metabolismo
Ratos
Receptores de GABA-A/metabolismo
Fatores de Troca de Nucleotídeo Guanina Rho/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arhgef9 protein, rat); 0 (Carrier Proteins); 0 (Membrane Proteins); 0 (Phosphatidylinositol Phosphates); 0 (Receptors, GABA-A); 0 (Rho Guanine Nucleotide Exchange Factors); 0 (gephyrin); 0 (phosphatidylinositol 3-phosphate); EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.771592


  9 / 5596 MEDLINE  
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[PMID]:27852895
[Au] Autor:Szíber Z; Liliom H; Morales CO; Ignácz A; Rátkai AE; Ellwanger K; Link G; Szucs A; Hausser A; Schlett K
[Ad] Endereço:Department of Physiology and Neurobiology, Eötvös Loránd University, H-1117 Budapest, Hungary.
[Ti] Título:Ras and Rab interactor 1 controls neuronal plasticity by coordinating dendritic filopodial motility and AMPA receptor turnover.
[So] Source:Mol Biol Cell;28(2):285-295, 2017 Jan 15.
[Is] ISSN:1939-4586
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ras and Rab interactor 1 (RIN1) is predominantly expressed in the nervous system. RIN1-knockout animals have deficits in latent inhibition and fear extinction in the amygdala, suggesting a critical role for RIN1 in preventing the persistence of unpleasant memories. At the molecular level, RIN1 signals through Rab5 GTPases that control endocytosis of cell-surface receptors and Abl nonreceptor tyrosine kinases that participate in actin cytoskeleton remodeling. Here we report that RIN1 controls the plasticity of cultured mouse hippocampal neurons. Our results show that RIN1 affects the morphology of dendritic protrusions and accelerates dendritic filopodial motility through an Abl kinase-dependent pathway. Lack of RIN1 results in enhanced mEPSC amplitudes, indicating an increase in surface AMPA receptor levels compared with wild-type neurons. We further provide evidence that the Rab5 GEF activity of RIN1 regulates surface GluA1 subunit endocytosis. Consequently loss of RIN1 blocks surface AMPA receptor down-regulation evoked by chemically induced long-term depression. Our findings indicate that RIN1 destabilizes synaptic connections and is a key player in postsynaptic AMPA receptor endocytosis, providing multiple ways of negatively regulating memory stabilization during neuronal plasticity.
[Mh] Termos MeSH primário: Proteínas rab de Ligação ao GTP/metabolismo
Proteínas rab de Ligação ao GTP/fisiologia
[Mh] Termos MeSH secundário: Animais
Movimento Celular/fisiologia
Dendritos/metabolismo
Dendritos/fisiologia
Endocitose/fisiologia
Hipocampo/fisiologia
Peptídeos e Proteínas de Sinalização Intracelular/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Plasticidade Neuronal/fisiologia
Neurônios/metabolismo
Proteínas Proto-Oncogênicas c-abl/metabolismo
Pseudópodes/metabolismo
Pseudópodes/fisiologia
Receptores de AMPA/metabolismo
Receptores de AMPA/fisiologia
Transdução de Sinais/fisiologia
Membranas Sinápticas/fisiologia
Proteínas rab5 de Ligação ao GTP/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Intracellular Signaling Peptides and Proteins); 0 (Receptors, AMPA); 0 (Rin1 protein, mouse); EC 2.7.10.2 (Proto-Oncogene Proteins c-abl); EC 3.6.5.2 (rab GTP-Binding Proteins); EC 3.6.5.2 (rab5 GTP-Binding Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161118
[St] Status:MEDLINE
[do] DOI:10.1091/mbc.E16-07-0526


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[PMID]:27565350
[Au] Autor:Loh KH; Stawski PS; Draycott AS; Udeshi ND; Lehrman EK; Wilton DK; Svinkina T; Deerinck TJ; Ellisman MH; Stevens B; Carr SA; Ting AY
[Ad] Endereço:Department of Chemistry, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.
[Ti] Título:Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts.
[So] Source:Cell;166(5):1295-1307.e21, 2016 Aug 25.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cellular compartments that cannot be biochemically isolated are challenging to characterize. Here we demonstrate the proteomic characterization of the synaptic clefts that exist at both excitatory and inhibitory synapses. Normal brain function relies on the careful balance of these opposing neural connections, and understanding how this balance is achieved relies on knowledge of their protein compositions. Using a spatially restricted enzymatic tagging strategy, we mapped the proteomes of two of the most common excitatory and inhibitory synaptic clefts in living neurons. These proteomes reveal dozens of synaptic candidates and assign numerous known synaptic proteins to a specific cleft type. The molecular differentiation of each cleft allowed us to identify Mdga2 as a potential specificity factor influencing Neuroligin-2's recruitment of presynaptic neurotransmitters at inhibitory synapses.
[Mh] Termos MeSH primário: Moléculas de Adesão Celular Neuronais/metabolismo
Neurônios GABAérgicos/metabolismo
Imunoglobulinas/metabolismo
Glicoproteínas de Membrana/metabolismo
Proteínas do Tecido Nervoso/metabolismo
Proteoma/metabolismo
Membranas Sinápticas/metabolismo
[Mh] Termos MeSH secundário: Animais
Antígenos CD/metabolismo
Ácido Glutâmico/metabolismo
Células HEK293
Seres Humanos
Camundongos
Moléculas de Adesão de Célula Nervosa/metabolismo
Peroxidase/genética
Peroxidase/metabolismo
Proteômica
Ratos
Receptores de GABA/metabolismo
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Tálamo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Cell Adhesion Molecules, Neuronal); 0 (Immunoglobulins); 0 (MDGA1 protein, rat); 0 (MDGA2 protein, rat); 0 (Membrane Glycoproteins); 0 (Nerve Tissue Proteins); 0 (Neural Cell Adhesion Molecules); 0 (Proteome); 0 (Receptors, GABA); 0 (Recombinant Fusion Proteins); 0 (antigens, CD200); 0 (neuroligin 2); 3KX376GY7L (Glutamic Acid); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE



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