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[PMID]:29368595
[Au] Autor:Park J; Lee M
[Ad] Endereço:Department of Ophthalmology, College of Medicine, Dankook University, 119, Dandae-ro, Dnognam-gu, Cheonan-si, Chungchungnam-do, Republic of Korea.
[Ti] Título:Short-term effects and safety of an acute increase of intraocular pressure after intravitreal bevacizumab injection on corneal endothelial cells.
[So] Source:BMC Ophthalmol;18(1):17, 2018 Jan 25.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The purpose of this study is to evaluate short-term effects and safety of an acute increase of intraocular pressure (IOP) after single-dose intravitreal bevacizumab injection on corneal endothelial cells and central corneal thickness. METHODS: Forty-two patients who underwent intravitreal injection of 2.5 mg/0.1 ml bevacizumab because of central serous chorioretinopathy or diabetic macular edema were included in this study. The changes of IOP, corneal endothelial cells, and corneal thickness at baseline, 2 min, 5 min, and 30 min after injection were analyzed prospectively with a specular microscope. RESULTS: In all patients, the mean IOPs at baseline, 2 min, 5 min, and 30 min after injection were 11.48 ± 2.22 mmHg, 49.71 ± 10.73 mmHg, 37.64 ± 11.68 mmHg, and 14.88 ± 4.77 mmHg, respectively. These changes were significant (p < 0.01). In only one eye, IOP did not decrease to ≤30 mmHg even at 30 min after injection. According to changes in IOP with time, the coefficient of variation of the corneal endothelium significantly increased (p = 0.03), but cell density, hexagonality of the corneal endothelium, and central corneal thickness did not change (p = 0.79, 0.21, and 0.08, prospectively). One week after injection, there was no sign of inflammation or any other complications in all 42 eyes. CONCLUSIONS: After intravitreal injection, IOP rapidly increases, then decreases to the normal range in most eyes 30 min after injection and it is tolerable to corneal endothelium. TRIAL REGISTRATION: Clinical Research Information Service (CRiS), Republic of Korea, KCT0002645 . Retrospectively registered 9 January 2018.
[Mh] Termos MeSH primário: Bevacizumab/efeitos adversos
Epitélio Posterior/efeitos dos fármacos
Pressão Intraocular/efeitos dos fármacos
Degeneração Macular/tratamento farmacológico
Edema Macular/tratamento farmacológico
Hipertensão Ocular/induzido quimicamente
[Mh] Termos MeSH secundário: Doença Aguda
Inibidores da Angiogênese/administração & dosagem
Inibidores da Angiogênese/efeitos adversos
Bevacizumab/administração & dosagem
Epitélio Posterior/patologia
Feminino
Seguimentos
Seres Humanos
Incidência
Pressão Intraocular/fisiologia
Injeções Intravítreas
Degeneração Macular/diagnóstico
Edema Macular/diagnóstico
Masculino
Meia-Idade
Hipertensão Ocular/epidemiologia
Hipertensão Ocular/fisiopatologia
Estudos Prospectivos
República da Coreia/epidemiologia
Fatores de Tempo
Fator A de Crescimento do Endotélio Vascular/antagonistas & inibidores
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiogenesis Inhibitors); 0 (Vascular Endothelial Growth Factor A); 2S9ZZM9Q9V (Bevacizumab)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0682-9


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[PMID]:29338061
[Au] Autor:Okumura N; Matsumoto D; Fukui Y; Teramoto M; Imai H; Kurosawa T; Shimada T; Kruse F; Schlötzer-Schrehardt U; Kinoshita S; Koizumi N
[Ad] Endereço:Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan.
[Ti] Título:Feasibility of cell-based therapy combined with descemetorhexis for treating Fuchs endothelial corneal dystrophy in rabbit model.
[So] Source:PLoS One;13(1):e0191306, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Corneal transparency is maintained by the corneal endothelium through its pump and barrier function. Severe corneal endothelial damage results in dysregulation of water flow and eventually causes corneal haziness and deterioration of visual function. In 2013, we initiated clinical research of cell-based therapy for treating corneal decompensation. In that study, we removed an 8-mm diameter section of damaged corneal endothelium without removing Descemet's membrane (the basement membrane of the corneal endothelium) and then injected cultured human corneal endothelial cells (CECs) into the anterior chamber. However, Descemet's membrane exhibits clinically abnormal structural features [i.e., multiple collagenous excrescences (guttae) and thickening] in patients with Fuchs endothelial corneal dystrophy (FECD) and the advanced cornea guttae adversely affects the quality of vision, even in patients without corneal edema. The turnover time of cornea guttae is also not certain. Therefore, we used a rabbit model to evaluate the feasibility of Descemet's membrane removal in the optical zone only, by performing a small 4-mm diameter descemetorhexis prior to CEC injection. We showed that the corneal endothelium is regenerated both on the corneal stroma (the area of Descemet's membrane removal) and on the intact peripheral Descemet's membrane, based on the expression of function-related markers and the restoration of corneal transparency. Recovery of the corneal transparency and central corneal thickness was delayed in areas of Descemet's membrane removal, but the cell density of the regenerated corneal endothelium and the thickness of the central corneal did not differ between the areas with and without residual Descemet's membrane at 14 days after CEC injection. Here, we demonstrate that removal of a pathological Descemet's membrane by a small descemetorhexis is a feasible procedure for use in combination with cell-based therapy. The current strategy might be beneficial for improving visual quality after CEC injection as a treatment for FECD.
[Mh] Termos MeSH primário: Terapia Baseada em Transplante de Células e Tecidos
Transplante de Córnea
Distrofia Endotelial de Fuchs/terapia
[Mh] Termos MeSH secundário: Idoso
Animais
Terapia Combinada
Lâmina Limitante Posterior/cirurgia
Modelos Animais de Doenças
Epitélio Posterior/patologia
Estudos de Viabilidade
Feminino
Distrofia Endotelial de Fuchs/patologia
Distrofia Endotelial de Fuchs/cirurgia
Seres Humanos
Masculino
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191306


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[PMID]:29422754
[Au] Autor:Sahu PK; Das GK; Agrawal S; Kumar S; Kumar N
[Ad] Endereço:Department of Ophthalmology, UCMS and GTB Hospital, New Delhi, India.
[Ti] Título:Comparative Evaluation of Corneal Endothelium in Diabetic Patients Undergoing Phacoemulsification.
[So] Source:Middle East Afr J Ophthalmol;24(4):195-201, 2017 Oct-Dec.
[Is] ISSN:0975-1599
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To determine if phacoemulsification with intraocular lens implantation has a greater impact on the corneal endothelium of type 2 diabetic patients as compared to nondiabetics. MATERIALS AND METHODS: This study compared the endothelial status in 60 diabetics with good glycemic control and 60 nondiabetics before and after uneventful phacoemulsification. Central corneal endothelial cell density, percentage hexagonality and percentage coefficient of variation (%CV) were measured using a specular microscope. RESULTS: Data were age and sex matched. Diabetics showed significantly higher loss in endothelial cell count as compared to nondiabetics. At the end of 3 months, there was a decline of 157 endothelial cell (standard deviation [SD] 90) in the diabetic group and 121 (SD 50) in the control group. This was statistically significant ( = 0.008). Furthermore, the diabetics showed a slower recovery trend in the endothelial healing response as evidenced by lower change in the %CV. The change in %CV was 4.5 in the control group and 3.1 in diabetic group which was statistically significant ( = 0.016). Significant correlation was found between energy used and change in endothelial count as well as %CV in nondiabetics only. CONCLUSION: In spite of good glycemic control, diabetics have significantly more endothelial damage in comparison to nondiabetics with similar nuclear grading and phaco energy used. This warrants a more careful use of phaco energy in diabetics.
[Mh] Termos MeSH primário: Perda de Células Endoteliais da Córnea/diagnóstico
Diabetes Mellitus Tipo 2/complicações
Epitélio Posterior/patologia
Implante de Lente Intraocular
Facoemulsificação
Complicações Pós-Operatórias
[Mh] Termos MeSH secundário: Idoso
Glicemia/metabolismo
Contagem de Células
Perda de Células Endoteliais da Córnea/etiologia
Feminino
Hemoglobina A Glicada/metabolismo
Seres Humanos
Masculino
Meia-Idade
Estudos Prospectivos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Glycated Hemoglobin A); 0 (hemoglobin A1c protein, human)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE
[do] DOI:10.4103/meajo.MEAJO_212_16


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[PMID]:29218423
[Au] Autor:Chan JCW; Choy BNK; Chan OCC; Li KKW
[Ad] Endereço:Department of Ophthalmology, United Christian Hospital, 130 Hip Wo Street, Kowloon, Hong Kong.
[Ti] Título:Early intraocular pressure change after peripheral iridotomy with ultralow fluence pattern scanning laser and Nd:YAG laser in primary angle-closure suspect: Kowloon East Pattern Scanning Laser Study Report No. 3.
[So] Source:Graefes Arch Clin Exp Ophthalmol;256(2):363-369, 2018 Feb.
[Is] ISSN:1435-702X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Our purpose was to assess the early intraocular pressure (IOP) changes of ultralow fluence laser iridotomy using pattern scanning laser followed by neodymium:yttrium-aluminum-gamet (Nd:YAG) laser. METHODS: This is a prospective interventional study. Thirty-three eyes of 33 adult Chinese primary angle-closure suspect subjects were recruited for prophylactic laser peripheral iridotomy. Sequential laser peripheral iridotomy was performed using pattern scanning laser followed by Nd:YAG laser. Visual acuity (VA) and IOP were measured before treatment, at 1 h, 1 day, 1 week, 1 month, 3 months and 6 months after laser. Laser energy used and complications were documented. Corneal endothelial cell count was examined at baseline and 6 months. Patency of the iridotomy was assessed at each follow-up visit. RESULTS: All subjects achieved patent iridotomy in a single session. The mean energy used was 0.335+/-0.088 J for the pattern scanning laser, and 4.767+/-5.780 mJ for the Nd:YAG laser. The total mean energy was 0.339+/-0.089 J. None of the eyes developed a clinically significant IOP spike (≥ 8 mmHg) at 1 h and 1 day after laser use. Only four eyes developed higher IOP at 1 h and all were ≤3 mmHg compared to baseline. The mean IOP was 13.8+/-2.5 mmHg at 1 h and 11.5+/-2.2 mmHg at 1 day, both were significantly lower than baseline (15.8+/-2.1 mmHg) (P < 0.001). Mean VA (logMAR) was similar at 1 h post laser compared to baseline (0.23 vs 0.26). There was also no statistically significant difference in mean VA at other follow-up visits compared to baseline. Peripheral iridotomy closure was encountered in two (6.1%) eyes, one at 1 month and another at 6 months follow-up. There were no complications including hyphema, peripheral anterior synechia formation nor prolonged inflammation throughout the follow-up period. There was no significant loss in corneal endothelial cell counts at 6 months (2255+/-490) compared to baseline (2303+/-386) (P = 0.347). CONCLUSIONS: Sequential LPI using an ultralow fluence pattern scanning laser, followed by a Nd:YAG laser, is safe and efficacious, and produces no IOP spike in dark irides of primary angle-closure suspects. Further studies to investigate its role in the treatment of other angle-closure conditions are warranted.
[Mh] Termos MeSH primário: Humor Aquoso/fisiologia
Glaucoma de Ângulo Fechado/cirurgia
Pressão Intraocular/fisiologia
Iris/cirurgia
Terapia a Laser/métodos
Lasers de Estado Sólido/uso terapêutico
Acuidade Visual
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Epitélio Posterior/patologia
Feminino
Seguimentos
Glaucoma de Ângulo Fechado/diagnóstico
Glaucoma de Ângulo Fechado/fisiopatologia
Hong Kong
Seres Humanos
Masculino
Meia-Idade
Período Pós-Operatório
Estudos Prospectivos
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:CLINICAL STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1007/s00417-017-3860-1


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[PMID]:28742617
[Au] Autor:Dimitry MES; Lewis AD; Zacharaki F; Chandran M; Robson D; Menon G; Poole TRG
[Ad] Endereço:The Eye Dept, Frimley Park Hospital, Frimley Health NHS Foundation Trust, Surrey, United Kingdom.
[Ti] Título:Simple Single-Pass Technique for Ultrathin Descemet Stripping Automated Endothelial Keratoplasty: A Pilot Study.
[So] Source:Cornea;36(10):1178-1183, 2017 Oct.
[Is] ISSN:1536-4798
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To assess intraoperative and postoperative graft thickness (GT) after donor deturgescence for ultrathin Descemet stripping automated endothelial keratoplasty and to evaluate visual outcomes, endothelial cell density, and patient satisfaction at 1 year. METHODS: Prospective interventional case series of patients with Fuchs endothelial dystrophy, Fuchs endothelial dystrophy and cataract, and pseudophakic bullous keratopathy (n = 12 grafts). The donor cornea was allowed to thin out by simple evaporation on an artificial anterior chamber, to the required precut thickness, before a single microkeratome pass. GT after microkeratome cut, at 1 week, 1, 3, 6, and 12, months was measured. Early Treatment Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity, Pelli-Robson contrast sensitivity, endothelial cell density, and score on the visual function questionnaire (VFQ-25) were assessed. RESULTS: Mean intraoperative postmicrokeratome cut GT was 78.9 ± 33.3 µm. Mean GT at 1 week, 1, 3, 6, and 12 months was 70.7, 70.9, 62.8, 66.5, and 58.9 µm, respectively. Mean initial donor corneal thickness was 647 ± 67 µm, and mean precut thickness was 526 ± 4.5 µm (mean thinning time: 17 min). Best-corrected visual acuity at 1 week, 1, 3, 6, and 12 months was 68.8, 76.9, 76.3, 76.9, and 78.6 letters with 9-letter gain at 12 months (P = 0.02). Mean endothelial cell loss at 3, 6, and 12 months was 36.8% ± 6.75%, 37.2% ± 8%, and 37.9% ± 9.75% loss, respectively. At 1 year, 83.3% of patients achieved ≥20/40 (6/12) and 66.7% of patients achieved ≥20/32 (6/9.5). VFQ-25 testing showed an improvement in the visual function. CONCLUSIONS: This pilot study demonstrates a simple graft deturgescence technique that reproducibly creates ultrathin grafts without donor wastage.
[Mh] Termos MeSH primário: Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos
Distrofia Endotelial de Fuchs/cirurgia
[Mh] Termos MeSH secundário: Idoso
Sensibilidades de Contraste/fisiologia
Paquimetria Corneana
Epitélio Posterior/patologia
Epitélio Posterior/transplante
Feminino
Seres Humanos
Período Intraoperatório
Masculino
Meia-Idade
Projetos Piloto
Período Pós-Operatório
Estudos Prospectivos
Doadores de Tecidos
Tomografia de Coerência Óptica
Acuidade Visual/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1097/ICO.0000000000001273


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[PMID]:29208829
[Au] Autor:Maggon R; Bhattacharjee R; Shankar S; Kar RC; Sharma V; Roy S
[Ad] Endereço:Department of Ophthalmology, Command Hospital, Kolkata, West Bengal, India.
[Ti] Título:Comparative analysis of endothelial cell loss following phacoemulsification in pupils of different sizes.
[So] Source:Indian J Ophthalmol;65(12):1431-1435, 2017 Dec.
[Is] ISSN:1998-3689
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To compare Endothelial cell(EC) loss following Phacoemulsification (PKE) in pupils of different sizes. METHODS: A prospective double masked observational study in which a total of 150 eyes of 150 patients between 50 & 70 years of age with senile cataract of nuclear sclerosis grade II were enrolled. Patients were allocated into three groups of 50 eyes each in Group A (pupil size <5 mm), Group B (pupil size 5-7 mm) and Group C (pupil size >7 mm). Pupillary size was measured by determining the height of slit on slit-lamp biomicroscope examination. PKE was done by the same expert surgeon using vertical chop technique and a foldable intraocular lens was implanted in the capsular bag. Corneal EC count and pachymetry were performed twice and average of 2 readings was taken for the purpose of this study. Measurements were taken preoperatively and postoperatively on day 1, day 7 and day 30. RESULTS: The mean EC count loss on postoperative day 1 in Group A was 19.45%, Group B 14.89%, Group C 10.19% with statistical significant difference between Group A and Group B, as also Group A and Group C. The difference was not significant between Group B and Group C, though there was a fall in EC count in Group C as well. Increase in corneal thickness on postoperative day 1 in group A was 5.43%, Group B 3.55%, Group C 2.14% with statistical significant difference between Group A and Group B, as also Group A and Group C with no difference in Group B and Group C. CONCLUSION: PKE done in eyes with maximal pupillary dilatation of <5 mm causes a greater EC loss and results in thicker corneas postoperatively as compared to eyes with pupillary dilatation of >5 mm at the end of one month.
[Mh] Termos MeSH primário: Doenças da Córnea/diagnóstico
Epitélio Posterior/patologia
Facoemulsificação/efeitos adversos
Complicações Pós-Operatórias/diagnóstico
Pupila
[Mh] Termos MeSH secundário: Idoso
Contagem de Células
Doenças da Córnea/etiologia
Método Duplo-Cego
Feminino
Seguimentos
Seres Humanos
Masculino
Meia-Idade
Complicações Pós-Operatórias/etiologia
Estudos Prospectivos
Microscopia com Lâmpada de Fenda/métodos
Fatores de Tempo
Acuidade Visual
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.4103/ijo.IJO_730_17


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[PMID]:29260197
[Au] Autor:Wertheimer C; Kassumeh S; Piravej NP; Nilmayer O; Braun C; Priglinger C; Luft N; Wolf A; Mayer WJ; Priglinger SG; Eibl-Lindner KH
[Ad] Endereço:Department of ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
[Ti] Título:The Intraocular Lens as a Drug Delivery Device: In Vitro Screening of Pharmacologic Substances for the Prophylaxis of Posterior Capsule Opacification.
[So] Source:Invest Ophthalmol Vis Sci;58(14):6408-6418, 2017 Dec 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: Numerous pharmacologic substances have been proposed for preventing posterior capsule opacification (PCO). The following trial was to compare those drugs to find more suitable options. IOL should then be modified by the pharmaceuticals as a drug-delivery device. Methods: A systematic literature search was performed to identify published substances. FHL-124 was used to determine cell proliferation and toxicity using a dye reduction test (XTT). Prescreened substances showing a reduction on cell growth without being toxic were soaked into an IOL. Those IOL were tested for their effect on PCO in an anterior-segment model and the human ex vivo capsular bag model. Toxicity on a corneal endothelial cell line (CEC-SV40) was determined. Release kinetics of methotrexate from the IOL was measured. Toxicity testing in both cell lines was done in serum-free conditions. All growth assays were exposed to 10% fetal calf serum (FCS)-supplemented medium. Results: The substances inhibited cell growth at the following EC50: caffeic acid phenethyl ester 1.6 ± 0.9 nM, disulfiram 359 ± 33 nM, methotrexate 98.0 ± 29.7 nM, rapamycin 70.2 ± 14.0 pM, and retinoic acid 1.1 ± 0.12 nM. All but disulfiram showed an effect in the anterior segment model when soaked into an IOL. Long-term inhibitory effects in the human capsular bag model were observed for caffeic acid phenethyl ester and methotrexate IOLs. Only methotrexate and disulfiram did not show any toxicity on endothelial cells. Methotrexate was released constantly from the hydrophilic IOL for 2 weeks. Conclusions: We could identify caffeic acid phenethyl ester and methotrexate in vitro as potential candidates for IOL modification for PCO prophylaxis.
[Mh] Termos MeSH primário: Opacificação da Cápsula/tratamento farmacológico
Sistemas de Liberação de Medicamentos/métodos
Lentes Intraoculares
Medicamentos sob Prescrição/administração & dosagem
[Mh] Termos MeSH secundário: Adulto
Idoso
Segmento Anterior do Olho/efeitos dos fármacos
Cadáver
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Células Endoteliais/efeitos dos fármacos
Epitélio Posterior/citologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Medicamentos sob Prescrição/farmacocinética
Medicamentos sob Prescrição/farmacologia
Medicamentos sob Prescrição/toxicidade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Prescription Drugs)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22555


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[PMID]:28465186
[Au] Autor:Marquez-Curtis LA; McGann LE; Elliott JAW
[Ad] Endereço:Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada. Electronic address: marquezc@ualberta.ca.
[Ti] Título:Expansion and cryopreservation of porcine and human corneal endothelial cells.
[So] Source:Cryobiology;77:1-13, 2017 Aug.
[Is] ISSN:1090-2392
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Impairment of the corneal endothelium causes blindness that afflicts millions worldwide and constitutes the most often cited indication for corneal transplants. The scarcity of donor corneas has prompted the alternative use of tissue-engineered grafts which requires the ex vivo expansion and cryopreservation of corneal endothelial cells. The aims of this study are to culture and identify the conditions that will yield viable and functional corneal endothelial cells after cryopreservation. Previously, using human umbilical vein endothelial cells (HUVECs), we employed a systematic approach to optimize the post-thaw recovery of cells with high membrane integrity and functionality. Here, we investigated whether improved protocols for HUVECs translate to the cryopreservation of corneal endothelial cells, despite the differences in function and embryonic origin of these cell types. First, we isolated endothelial cells from pig corneas and then applied an interrupted slow cooling protocol in the presence of dimethyl sulfoxide (Me SO), with or without hydroxyethyl starch (HES). Next, we isolated and expanded endothelial cells from human corneas and applied the best protocol verified using porcine cells. We found that slow cooling at 1 °C/min in the presence of 5% Me SO and 6% HES, followed by rapid thawing after liquid nitrogen storage, yields membrane-intact cells that could form monolayers expressing the tight junction marker ZO-1 and cytoskeleton F-actin, and could form tubes in reconstituted basement membrane matrix. Thus, we show that a cryopreservation protocol optimized for HUVECs can be applied successfully to corneal endothelial cells, and this could provide a means to address the need for off-the-shelf cryopreserved cells for corneal tissue engineering and regenerative medicine.
[Mh] Termos MeSH primário: Criopreservação/métodos
Células Endoteliais
Epitélio Posterior/citologia
[Mh] Termos MeSH secundário: Actinas/metabolismo
Idoso
Animais
Crioprotetores/farmacologia
Dimetil Sulfóxido/farmacologia
Feminino
Seres Humanos
Derivados de Hidroxietil Amido/farmacologia
Masculino
Meia-Idade
Suínos
Proteína da Zônula de Oclusão-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Cryoprotective Agents); 0 (Hydroxyethyl Starch Derivatives); 0 (TJP1 protein, human); 0 (Zonula Occludens-1 Protein); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  9 / 5525 MEDLINE  
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[PMID]:27860476
[Au] Autor:Studený P; Krízová D; Kuchynka P
[Ti] Título:[Historic Survey of Posterior Lamellar Keratoplasty Techniques - an Overview].
[Ti] Título:Historický prehled technik zadní lamelární keratoplastiky..
[So] Source:Cesk Slov Oftalmol;72(4):112-118, 2016.
[Is] ISSN:1211-9059
[Cp] País de publicação:Czech Republic
[La] Idioma:cze
[Ab] Resumo:Some of the modifications of posterior lamellar keratoplasty is at the present considered by most of the corneal surgeons as method of choice in case of patients corneal endothelium damage. However, this surgical technique undergoes in the last 20 years relatively rapid development. Even in the current time, there exist side by side and are used different types and modifications of this sort of transplantation. Because of this reason, differences among individual methods are sometimes not accurately understood and the terminology may be used not exactly as well. The authors specify all until now described surgical methods, describe differences among single surgical procedures, and point out their advantages and disadvantages. The methods are described in the same order as they were historically introduced into the literature.Key words: posterior lamellar keratoplasty, surgical methods.
[Mh] Termos MeSH primário: Doenças da Córnea/história
Transplante de Córnea/história
Transplante de Córnea/métodos
[Mh] Termos MeSH secundário: Doenças da Córnea/cirurgia
Epitélio Posterior/patologia
Epitélio Posterior/cirurgia
História do Século XX
História do Século XXI
Seres Humanos
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


  10 / 5525 MEDLINE  
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[PMID]:29073200
[Au] Autor:Sorrentino FS; Matteini S; Imburgia A; Bonifazzi C; Sebastiani A; Parmeggiani F
[Ad] Endereço:Department of Surgical Sciences, Unit of Ophthalmology, Ospedale Maggiore, Bologna, Italy.
[Ti] Título:Torsional phacoemulsification: A pilot study to revise the "harm scale" evaluating the endothelial damage and the visual acuity after cataract surgery.
[So] Source:PLoS One;12(10):e0186975, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To study the effect of torsional phacoemulsification energy on corneal endothelium evaluating the relationship between changes of endothelial cells and postoperative visual acuity. METHODS: This prospective clinical observational cohort study included 50 patients with cataract who underwent torsional phacoemulsification. Sequential quantitative and qualitative morphometric endothelial cell analyses of the cornea were performed four weeks preoperatively and six weeks postoperatively using noncontact specular microscopy. RESULTS: This work confirmed the strong relationship, described by a linear model (one-way ANOVA, R2 = 77.9%, P < 0.0001), between the percentage of endothelial cell loss (ECL%) and the 5-score harm scale. According to the Tukey post-hoc pairwise comparison test, distinct values of ECL% are grouped in 3 subsets. The value of ECL = 10% has been identified as cut-off to discriminate patients with excellent postoperative best-corrected visual acuity (BCVA > 85 letters) from those with just a good/satisfied visual outcome (BCVA ≤ 85 letters). Within the 5-score harm scale, there was a significant correlation among phaco energy intraoperatively delivered and the average endothelial cell loss. CONCLUSIONS: This study confirms the validity of the 5-score harm scale first proposed by Sorrentino and colleagues in 2016. This time, the method categorizes cataracts taking into account nucleus hardness and phaco cumulative dissipated energy. Predicting the harm on corneal endothelium, we can discriminate patients with excellent BCVA and with just good/satisfied BCVA. With torsional phacoemulsification with respect to longitudinal, the percentage of patients who can reach excellent BCVA is remarkably increased.
[Mh] Termos MeSH primário: Epitélio Posterior/patologia
Facoemulsificação/efeitos adversos
Acuidade Visual
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Masculino
Meia-Idade
Projetos Piloto
Estudos Prospectivos
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186975



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