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[PMID]:29465543
[Au] Autor:Lin X; Wu Y; Fu Y; Dai Q
[Ad] Endereço:School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University.
[Ti] Título:Spontaneous reattachment of Descemet membrane detachment after deep anterior lamellar keratoplasty: A case report.
[So] Source:Medicine (Baltimore);97(8):e0032, 2018 Feb.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: To report a case of late spontaneous reattachment of Descemet membrane (DM) detachment after deep anterior lamellar keratoplasty (DALK). PATIENT CONCERNS: A 50-year-old woman with stromal corneal scarring underwent a DALK with the big bubble technique. The DM detachment and double anterior chamber were observed on the first day after operation. DIAGNOSES: Descemet membrane detachment after deep anterior lamellar keratoplasty [Oculus Dexter (OD), indicates right eye]. INTERVENTIONS: Sterile air injection was unsuccessful. Conservative observation was performed. OUTCOMES: During the follow-up, the donor cornea kept transparent and no edema was observed. The DM reattached spontaneously at the fifth months post-DALK. LESSONS: The present case suggests that the retained host DM may result in DM detachment after DALK. For the DM detachment without roll rim, the detachment may spontaneously reattach in several months after surgery.
[Mh] Termos MeSH primário: Transplante de Córnea/efeitos adversos
Lâmina Limitante Posterior/fisiopatologia
[Mh] Termos MeSH secundário: Transplante de Córnea/métodos
Lâmina Limitante Posterior/cirurgia
Feminino
Seres Humanos
Meia-Idade
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180222
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000010032


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[PMID]:29338061
[Au] Autor:Okumura N; Matsumoto D; Fukui Y; Teramoto M; Imai H; Kurosawa T; Shimada T; Kruse F; Schlötzer-Schrehardt U; Kinoshita S; Koizumi N
[Ad] Endereço:Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan.
[Ti] Título:Feasibility of cell-based therapy combined with descemetorhexis for treating Fuchs endothelial corneal dystrophy in rabbit model.
[So] Source:PLoS One;13(1):e0191306, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Corneal transparency is maintained by the corneal endothelium through its pump and barrier function. Severe corneal endothelial damage results in dysregulation of water flow and eventually causes corneal haziness and deterioration of visual function. In 2013, we initiated clinical research of cell-based therapy for treating corneal decompensation. In that study, we removed an 8-mm diameter section of damaged corneal endothelium without removing Descemet's membrane (the basement membrane of the corneal endothelium) and then injected cultured human corneal endothelial cells (CECs) into the anterior chamber. However, Descemet's membrane exhibits clinically abnormal structural features [i.e., multiple collagenous excrescences (guttae) and thickening] in patients with Fuchs endothelial corneal dystrophy (FECD) and the advanced cornea guttae adversely affects the quality of vision, even in patients without corneal edema. The turnover time of cornea guttae is also not certain. Therefore, we used a rabbit model to evaluate the feasibility of Descemet's membrane removal in the optical zone only, by performing a small 4-mm diameter descemetorhexis prior to CEC injection. We showed that the corneal endothelium is regenerated both on the corneal stroma (the area of Descemet's membrane removal) and on the intact peripheral Descemet's membrane, based on the expression of function-related markers and the restoration of corneal transparency. Recovery of the corneal transparency and central corneal thickness was delayed in areas of Descemet's membrane removal, but the cell density of the regenerated corneal endothelium and the thickness of the central corneal did not differ between the areas with and without residual Descemet's membrane at 14 days after CEC injection. Here, we demonstrate that removal of a pathological Descemet's membrane by a small descemetorhexis is a feasible procedure for use in combination with cell-based therapy. The current strategy might be beneficial for improving visual quality after CEC injection as a treatment for FECD.
[Mh] Termos MeSH primário: Terapia Baseada em Transplante de Células e Tecidos
Transplante de Córnea
Distrofia Endotelial de Fuchs/terapia
[Mh] Termos MeSH secundário: Idoso
Animais
Terapia Combinada
Lâmina Limitante Posterior/cirurgia
Modelos Animais de Doenças
Epitélio Posterior/patologia
Estudos de Viabilidade
Feminino
Distrofia Endotelial de Fuchs/patologia
Distrofia Endotelial de Fuchs/cirurgia
Seres Humanos
Masculino
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191306


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[PMID]:29252983
[Au] Autor:Li S; Liu L; Wang W; Huang T; Zhong X; Yuan J; Liang L
[Ad] Endereço:State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
[Ti] Título:Efficacy and safety of Descemet's membrane endothelial keratoplasty versus Descemet's stripping endothelial keratoplasty: A systematic review and meta-analysis.
[So] Source:PLoS One;12(12):e0182275, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Based on current evidence, the efficiency and safety of Descemet's membrane endothelial keratoplasty (DMEK) was compared with that of Descemet's stripping endothelial keratoplasty (DSEK). METHODS: Pubmed, Embase, Web of Science, the Cochrane Database and conference abstracts were comprehensively searched for studies that compared the efficacy and safety of DMEK and DSEK. The efficacy outcome was the postoperative best-corrected visual acuity (BCVA). The safety outcomes included the postoperative endothelial cell density (ECD) and complications such as graft detachment, graft rejection, graft failure, postoperative elevated intraocular pressure (IOP), tissue loss, etc. The outcomes were pooled using random-effects models with Stata 13.0 software. Heterogeneity was qualified with Q statistic and I2/H2 statistic. Publication bias was assessed using funnel plot, Begg rank correlation test, and Egger or Horbard linear regression. RESULTS: 19 articles were eligible, and 1124 eyes and 1254 eyes were included in the DMEK and DSEK groups, respectively. The overall pooled estimates showed a significantly better postoperative BCVA, a comparable ECD and an increased graft detachment rate in the DMEK group compared with the DSEK group (BCVA: mean difference (MD) = -0.15, 95% CI = -0.19 to -0.11, P<0.001; ECD: MD = 14.88, 95% CI = -181.50 to 211.27, P = 0.882; graft detachment rate: OR = 4.56, 95% CI = 2.43 to 8.58, P<0.001). Except for the postoperative ECD, which was changed to be higher in the DSEK group than the DMEK group, the learning curve did not have a marked effect on the comparison outcome of the BCVA and graft detachment rate based on the estimates pooled from studies that collected data during the DMEK learning phase (ECD (learning curve): MD = -361.24, 95% CI = -649.42 to -73.07, P = 0.014). CONCLUSION: Although DMEK is a more technically difficult and challenging procedure, it may represent a safe and more efficient alternative to DSEK for the treatment of corneal endothelial diseases, even during its learning curve.
[Mh] Termos MeSH primário: Lâmina Limitante Posterior/cirurgia
Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/efeitos adversos
Células Endoteliais/patologia
[Mh] Termos MeSH secundário: Contagem de Células
Lâmina Limitante Posterior/fisiopatologia
Seguimentos
Rejeição de Enxerto/etiologia
Seres Humanos
Pressão Intraocular
Cuidados Pós-Operatórios
Complicações Pós-Operatórias/etiologia
Viés de Publicação
Resultado do Tratamento
Acuidade Visual
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182275


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[PMID]:27777123
[Au] Autor:Schwarz C; Aldrich BT; Burckart KA; Schmidt GA; Zimmerman MB; Reed CR; Greiner MA; Sander EA
[Ad] Endereço:Department of Biomedical Engineering, University of Iowa, Iowa City, IA, USA.
[Ti] Título:Descemet membrane adhesion strength is greater in diabetics with advanced disease compared to healthy donor corneas.
[So] Source:Exp Eye Res;153:152-158, 2016 Dec.
[Is] ISSN:1096-0007
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Descemet membrane endothelial keratoplasty (DMEK) is an increasingly popular surgical procedure for treating ocular diseases that require a corneal transplant. Previous studies have found that tissue tearing during surgical preparation is more likely elevated in eyes from donors with a history of diabetes mellitus. To quantify these potential differences, we established an experimental technique for quantifying the force required to separate the endothelium-Descemet membrane complex (EDM) from stroma in human donor corneal tissue, and we assessed differences in adhesion strength between diabetic and non-diabetic donor corneas. Transplant suitable corneas were obtained from 23 donors 50-75 years old with an average preservation to assay time of 11.5 days. Corneas were classified from a medical records review as non-diabetic (ND, n = 9), diabetic without evidence of advanced disease (NAD, n = 8), or diabetic with evidence of advanced disease (AD, n = 10). Corneas were sectioned into 3 mm wide strips and the EDM peeled from the stroma. Using the force-extension data obtained from mechanical peel testing, EDM elastic peel tension (T ), elastic stiffness (S ), average delamination tension (T ), and maximum tension (T ) were calculated. Mean T , S , T , and T values for ND corneas were 0.78 ± 0.07 mN/mm, 0.37 ± 0.05 mN/mm/mm, 0.78 ± 0.08 mN/mm, and 0.94 ± 0.17 mN/mm, respectively. NAD values did not differ significantly. However, AD values for T (1.01 ± 0.18 mN/mm), T (1.09 ± 0.21 mN/mm), and T (1.37 ± 0.24 mN/mm) were greater than ND and NAD corneas (P < 0.05). S did not differ significantly between groups. These findings provide proof of the concept that chronic hyperglycemia from diabetes mellitus results in a phenotypically more adhesive interface between Descemet membrane and the posterior stroma in donor corneal tissue. Results of this study provide a foundation for further investigations into the impact of diabetes on the posterior cornea, eye banking, and keratoplasty.
[Mh] Termos MeSH primário: Doenças da Córnea/cirurgia
Lâmina Limitante Posterior/fisiologia
Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos
Diabetes Mellitus
Doadores de Tecidos
[Mh] Termos MeSH secundário: Idoso
Doenças da Córnea/fisiopatologia
Bancos de Olhos
Sobrevivência de Enxerto
Seres Humanos
Meia-Idade
Reprodutibilidade dos Testes
Coleta de Tecidos e Órgãos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


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[PMID]:28936052
[Au] Autor:Khairallah AS
[Ad] Endereço:Department of Surgery (Ophthalmology), College of Medicine, King Khalid University, Abha, Saudi Arabia.
[Ti] Título:Outcome of Descemet Stripping Automated Endothelial Keratoplasty in Failed Penetrating Keratoplasty.
[So] Source:Middle East Afr J Ophthalmol;24(2):87-90, 2017 Apr-Jun.
[Is] ISSN:0975-1599
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The purpose of this study was to report the outcomes of descemet stripping automated endothelial keratoplasty (DSAEK) surgeries in eyes with failed penetrating keratoplasty (PKP). METHODS: This was a retrospective, noncomparative, descriptive case series. Patients who underwent DSAEK following PKP from January 2007 to December 2012 were reviewed. Data were collected on best-corrected visual acuity (BCVA) before and 1 week, 1 month, 3 months, 6 months, 1 year, and 2 years following DSAEK. Intra- and post-operative complications, changes in intraocular pressure (IOP) were also documented. Demographics were associated with the visual outcomes. RESULTS: Fifteen eyes of 15 patients with failed PKP comprised the study group. BCVA improved by 2 or more lines at 6 months after DSAEK in ten (66.6%) eyes. In the five eyes that did not experience an increase in BCVA, four had complications; detached lenticule (2 eyes), rejection (1 eye), and dislocation of the graft (1 eye). One eye had ocular comorbidity causing poor vision. The mean IOP at 12-15 weeks postoperatively was 17.4 ± 6.8 mmHg. CONCLUSION: DSAEK for failed PKP resulted in improved vision in two-third of cases. Selection of cases for this repeat surgery seems to be a key in successful outcomes.
[Mh] Termos MeSH primário: Doenças da Córnea/cirurgia
Lâmina Limitante Posterior/cirurgia
Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos
Ceratoplastia Penetrante/efeitos adversos
Complicações Pós-Operatórias
Acuidade Visual
[Mh] Termos MeSH secundário: Adulto
Idoso
Feminino
Seres Humanos
Masculino
Meia-Idade
Reoperação
Estudos Retrospectivos
Falha de Tratamento
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE
[do] DOI:10.4103/meajo.MEAJO_248_15


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[PMID]:28902856
[Au] Autor:Weber IP; Rana M; Thomas PBM; Dimov IB; Franze K; Rajan MS
[Ad] Endereço:Department of Physiology, Development and Neuroscience, Anatomy Building, University of Cambridge, Cambridge, United Kingdom.
[Ti] Título:Effect of vital dyes on human corneal endothelium and elasticity of Descemet's membrane.
[So] Source:PLoS One;12(9):e0184375, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The purpose of this study was to evaluate the effects of vital dyes on human Descemet's membranes (DMs) and endothelia. DMs of 25 human cadaveric corneas with research consent were treated with dyes routinely used in Descemet membrane endothelial keratoplasty (DMEK), 0.05% Trypan blue (TB) or a combination of 0.15% Trypan blue, 0.025% Brilliant blue and 4% Polyethylene glycol (commercial name Membrane Blue Dual; MB). The effects of these two dyes on (i) endothelial cell viability, (ii) DM mechanical properties as assessed by atomic force microscopy, and iii) qualitative DM dye retention were tested for two varying exposure times (one or four minutes). No significant differences in cell toxicity were observed between treatments with TB and MB at the two different exposure times (P = 0.21). Further, both dyes led to a significant increase in DM stiffness: exposure to TB and MB for one minute increased the apparent elastic modulus of the DM by 11.2% (P = 8*10-3) and 17.7%, respectively (P = 4*10-6). A four-minute exposure led to an increase of 8.6% for TB (P = 0.004) and 13.6% for MB (P = 0.03). Finally, at 25 minutes, the dye retention of the DM was considerably better for MB compared to TB. Taken together, a one-minute exposure to MB was found to improve DM visibility compared to TB, with a significant increase in DM stiffness and without detrimental effects on endothelial cell viability. The use of MB could therefore improve (i) visibility of the DM scroll, and (ii) intraoperative unfolding, enhancing the probability of successful DMEK surgery.
[Mh] Termos MeSH primário: Corantes/farmacologia
Lâmina Limitante Posterior/efeitos dos fármacos
Elasticidade/efeitos dos fármacos
Epitélio Posterior/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adulto
Idoso
Benzenossulfonatos/farmacologia
Cadáver
Sobrevivência Celular/efeitos dos fármacos
Córnea/efeitos dos fármacos
Córnea/patologia
Córnea/cirurgia
Lâmina Limitante Posterior/patologia
Lâmina Limitante Posterior/fisiologia
Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/efeitos adversos
Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos
Módulo de Elasticidade/efeitos dos fármacos
Epitélio Posterior/patologia
Epitélio Posterior/fisiologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Polietilenoglicóis/farmacologia
Resultado do Tratamento
Azul Tripano/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzenesulfonates); 0 (Coloring Agents); 30IQX730WE (Polyethylene Glycols); H3R47K3TBD (brilliant blue); I2ZWO3LS3M (Trypan Blue)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184375


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[PMID]:28850636
[Au] Autor:Bhogal M; Lwin CN; Seah XY; Peh G; Mehta JS
[Ad] Endereço:Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore.
[Ti] Título:Allogeneic Descemet's Membrane Transplantation Enhances Corneal Endothelial Monolayer Formation and Restores Functional Integrity Following Descemet's Stripping.
[So] Source:Invest Ophthalmol Vis Sci;58(10):4249-4260, 2017 Aug 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To characterize the differences in corneal endothelial wound healing in the presence or absence of Descemet's membrane (DM), in vivo. Methods: New-Zealand white rabbits were subjected to 7-mm endothelial wound either by scraping (n = 8; DM intact), peeling (n = 6; DM removed), or a combinatory scrape/peel wound (n = 6). In a second experiment, rabbits underwent peel wound with immediate transplantation of pure decellularized human DM (n = 4). In vivo endothelial migration was assessed via trypan blue staining. Recovery of corneal clarity and thickness was performed by using slit-lamp biomicroscopy and optical coherence tomography. Cell proliferation, phenotype, and morphology were assessed by using immunofluorescence and scanning electron microscopy. Results: In vivo wound closure was faster in the presence of DM; 25.4% ± 1.4%/d versus 5.5% ± 0.6%/d (P < 0.0001). At day 8, complete wound closure was seen in all of the scrape samples but none of the peel group, with wound closure preceding clinical recovery by approximately 6 days in the scrape group. Endothelial cells in the scraped areas reformed functional monolayers capable of restoring corneal thickness and transparency whilst those in the peeled area underwent mesenchymal-like transformation resulting in scar formation. Transplanting decellularized DM in animals receiving a peel wound resulted in clarity and thickness comparable to the scrape group. Endothelial proliferation (Ki67 +ve cells) was higher in scraped versus peeled areas: 54.7% ± 3.5% vs. 8.8% ± 0.7%, (P < 0.01). Conclusions: The presence of DM promoted endothelial wound healing, proliferation, and maintenance of a normal phenotype. DM transplantation recovered the abnormal peel phenotype back to that observed after endothelial scraping.
[Mh] Termos MeSH primário: Lesões da Córnea/cirurgia
Lâmina Limitante Posterior/cirurgia
Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos
Epitélio Posterior/cirurgia
[Mh] Termos MeSH secundário: Animais
Proliferação Celular/fisiologia
Lesões da Córnea/patologia
Lesões da Córnea/fisiopatologia
Modelos Animais de Doenças
Epitélio Posterior/citologia
Coelhos
Recuperação de Função Fisiológica/fisiologia
Transplante Homólogo
Cicatrização/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22106


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[PMID]:28727885
[Au] Autor:Okumura N; Kitahara M; Okuda H; Hashimoto K; Ueda E; Nakahara M; Kinoshita S; Young RD; Quantock AJ; Tourtas T; Schlötzer-Schrehardt U; Kruse F; Koizumi N
[Ad] Endereço:Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan.
[Ti] Título:Sustained Activation of the Unfolded Protein Response Induces Cell Death in Fuchs' Endothelial Corneal Dystrophy.
[So] Source:Invest Ophthalmol Vis Sci;58(9):3697-3707, 2017 Jul 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: The unfolded protein response (UPR) is believed to play a role in the pathogenesis of Fuchs' endothelial corneal dystrophy (FECD). The purpose of this study was to investigate whether unfolded proteins accumulate in the corneal endothelium in FECD and if they are involved in triggering cell death. Methods: Descemet's membranes with corneal endothelial cells (CECs) were obtained during keratoplasty, and expression of aggresomes, type 1 collagen, fibronectin, and agrin was evaluated. Endoplasmic reticulum (ER) stress of immortalized human CECs from non-FECD subjects and from FECD patients (iHCEC and iFECD, respectively) were evaluated. The effect of MG132-mediated aggresome formation on the UPR and intrinsic pathway and the effect of mitochondrial damage on UPR were also examined. The effect of CHOP knockdown on the ER stress-mediated intrinsic pathway was also evaluated. Results: Aggresome formation was higher in iFECD than in iHCEC and was colocalized with type 1 collagen, fibronectin, and agrin. GRP78, phosphorylated IRE1, PERK, and CHOP showed higher activation in iFECD than in iHCEC. MG132-mediated aggresome formation upregulated ER stress sensors, the mitochondrial membrane potential drop, cytochrome c release to the cytoplasm, and activation of caspase-9 and -3. By contrast, staurosporine-mediated mitochondrial damage did not induce ER stress. Knockdown of CHOP attenuated the ER stress-induced cleavage of caspase-9, which is caused by intrinsic pathway activation. Conclusions: Excessive synthesis of extracellular matrix proteins induced unfolded protein accumulation in FECD. Prolonged ER stress-mediated cell death, occurring via the intrinsic apoptotic signaling pathway, therefore might be associated with the pathogenesis of FECD.
[Mh] Termos MeSH primário: Apoptose
Epitélio Posterior/metabolismo
Proteínas da Matriz Extracelular/metabolismo
Distrofia Endotelial de Fuchs/patologia
Agregação Patológica de Proteínas/patologia
Resposta a Proteínas não Dobradas/fisiologia
[Mh] Termos MeSH secundário: Agrina/metabolismo
Células Cultivadas
Colágeno Tipo I/metabolismo
Lâmina Limitante Posterior/metabolismo
Lâmina Limitante Posterior/patologia
Retículo Endoplasmático/metabolismo
Retículo Endoplasmático/patologia
Fibronectinas/metabolismo
Distrofia Endotelial de Fuchs/metabolismo
Proteínas de Choque Térmico/metabolismo
Seres Humanos
Imuno-Histoquímica
Potencial da Membrana Mitocondrial/fisiologia
Meia-Idade
Estresse Oxidativo
Agregação Patológica de Proteínas/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Agrin); 0 (Collagen Type I); 0 (Extracellular Matrix Proteins); 0 (Fibronectins); 0 (Heat-Shock Proteins); 0 (molecular chaperone GRP78)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-21023


  9 / 1724 MEDLINE  
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[PMID]:28724824
[Au] Autor:D'Souza S; Solanki N; Sushma KR; Solanki P
[Ad] Endereço:Department of Cornea and Refractive Surgery, Dr. Solanki Eye Hospital, Malleswaram, Bengaluru, Karnataka, India.
[Ti] Título:Late onset Descemet's membrane detachment 20 years after penetrating keratoplasty.
[So] Source:Indian J Ophthalmol;65(7):621-623, 2017 Jul.
[Is] ISSN:1998-3689
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:This paper reports a case of spontaneous Descemet's Membrane (DM ) detachment 20-years after penetrating keratoplasty (PK). The patient presented with acute onset blurred vision in the right eye associated with mild ocular discomfort. There was no prior history of trauma. The patient had undergone PK in the right eye 20 years back. Detailed ophthalmic examination showed best corrected visual acuity of hand movements close to face in the right eye and 20/80 in the left eye. The right eye showed diffuse graft edema but no congestion, epithelial defect, or loss of continuity of graft-host junction to suggest a traumatic etiology. Anterior segment optical coherence tomography showed DM break with a detachment in the right eye, which was treated with an intracameral C3F8 injection. DM break with detachment post-PK has been very sparsely reported in literature and is an important differential to remember when faced with an edematous graft, in addition to the diagnosis of graft rejection or failure.
[Mh] Termos MeSH primário: Edema da Córnea/etiologia
Lâmina Limitante Posterior/patologia
Ceratoplastia Penetrante/efeitos adversos
Complicações Pós-Operatórias
[Mh] Termos MeSH secundário: Adulto
Edema da Córnea/diagnóstico
Lâmina Limitante Posterior/cirurgia
Seguimentos
Seres Humanos
Masculino
Fatores de Tempo
Tomografia de Coerência Óptica
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.4103/ijo.IJO_828_16


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[PMID]:28662635
[Au] Autor:Weng Y; Ren YP; Zhang L; Huang XD; Shen-Tu XC
[Ad] Endereço:Eye Center, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China.
[Ti] Título:An alternative technique for Descemet's membrane detachment following phacoemulsification: case report and review of literature.
[So] Source:BMC Ophthalmol;17(1):109, 2017 Jun 29.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Descemet's membrane detachment (DMD) is one of the most serious complications of modern cataract surgery. We present an alternative technique for management of DMD with a review of the literature on current strategies for the treatment of DMD. CASE PRESENTATION: A 74-year-old woman developed DMD after phacoemulsification and failed the first descemetopexy with air tamponade. An alternative method was used to drain the pre-descematic fluid and reposition the detached Descemet's membrane in this rare case. This technique involved completely filling the anterior chamber with an intracameral air injection, followed by using a 23-gauge needle to puncture the peripheral cornea to drain the pre-descematic fluid. The Descemet's membrane was completely reattached to the stroma during the follow-up. CONCLUSIONS: Drainage of pre-descematic fluid combined with intracameral air tamponading was used as an alternative surgical option for the management of this severe case of DMD.
[Mh] Termos MeSH primário: Edema da Córnea/cirurgia
Lâmina Limitante Posterior/cirurgia
Facoemulsificação/efeitos adversos
Complicações Pós-Operatórias
[Mh] Termos MeSH secundário: Idoso
Edema da Córnea/diagnóstico
Edema da Córnea/etiologia
Lâmina Limitante Posterior/patologia
Tamponamento Interno
Feminino
Seres Humanos
Tomografia de Coerência Óptica
Acuidade Visual
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-017-0506-3



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