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  1 / 4889 MEDLINE  
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[PMID]:29370308
[Au] Autor:Zhang M; Zhou Q; Luo Y; Nguyen T; Rosenblatt MI; Guaiquil VH
[Ad] Endereço:Department of Ophthalmology and Visual Sciences, University of Illinois-Chicago, Chicago, Illinois, United States of America.
[Ti] Título:Semaphorin3A induces nerve regeneration in the adult cornea-a switch from its repulsive role in development.
[So] Source:PLoS One;13(1):e0191962, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The peripheral sensory nerves that innervate the cornea can be easily damaged by trauma, surgery, infection or diabetes. Several growth factors and axon guidance molecules, such as Semaphorin3A (Sema3A) are upregulated upon cornea injury. Nerves can regenerate after injury but do not recover their original density and patterning. Sema3A is a well known axon guidance and growth cone repellent protein during development, however its role in adult cornea nerve regeneration remains undetermined. Here we investigated the neuro-regenerative potential of Sema3A on adult peripheral nervous system neurons such as those that innervate the cornea. First, we examined the gene expression profile of the Semaphorin class 3 family members and found that all are expressed in the cornea. However, upon cornea injury there is a fast increase in Sema3A expression. We then corroborated that Sema3A totally abolished the growth promoting effect of nerve growth factor (NGF) on embryonic neurons and observed signs of growth cone collapse and axonal retraction after 30 min of Sema3A addition. However, in adult isolated trigeminal ganglia or dorsal root ganglia neurons, Sema3A did not inhibited the NGF-induced neuronal growth. Furthermore, adult neurons treated with Sema3A alone produced similar neuronal growth to cells treated with NGF and the length of the neurites and branching was comparable between both treatments. These effects were replicated in vivo, where thy1-YFP neurofluorescent mice subjected to cornea epithelium debridement and receiving intrastromal pellet implantation containing Sema3A showed increased corneal nerve regeneration than those receiving pellets with vehicle. In adult PNS neurons, Sema3A is a potent inducer of neuronal growth in vitro and cornea nerve regeneration in vivo. Our data indicates a functional switch for the role of Sema3A in PNS neurons where the well-described repulsive role during development changes to a growth promoting effect during adulthood. The high expression of Sema3A in the normal and injured adult corneas could be related to its role as a growth factor.
[Mh] Termos MeSH primário: Regeneração Nervosa/efeitos dos fármacos
Semaforina-3A/farmacologia
[Mh] Termos MeSH secundário: Animais
Epitélio Anterior/efeitos dos fármacos
Epitélio Anterior/lesões
Epitélio Anterior/metabolismo
Gânglios Espinais/citologia
Gânglios Espinais/efeitos dos fármacos
Cones de Crescimento/efeitos dos fármacos
Camundongos
Nervo Trigêmeo/citologia
Nervo Trigêmeo/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Semaphorin-3A)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191962


  2 / 4889 MEDLINE  
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[PMID]:29301512
[Au] Autor:Qu JH; Li L; Tian L; Zhang XY; Thomas R; Sun XG
[Ad] Endereço:Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, 100730, China.
[Ti] Título:Epithelial changes with corneal punctate epitheliopathy in type 2 diabetes mellitus and their correlation with time to healing.
[So] Source:BMC Ophthalmol;18(1):1, 2018 Jan 04.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: To study basal epithelial cell (BEC), sub-basal nerve plexus (SBN) and Langerhans cell (LC) density in patients with type 2 diabetes mellitus (T2DM) with corneal punctate epitheliopathy (CPE) and to assess their association with time to healing of CPE. METHODS: Retrospective study of in vivo confocal microscopy (IVCM) in 160 eyes from 160 patients with T2DM diagnosed with CPE due to a single cause. Key exclusion criteria included multiple-causes for CPE or treatment with autologous serum. A total of 149 eyes from 149 gender- age- and aetiolgy-matched patients with CPE without T2DM comprised the control group. Electronic records were reviewed for demographic features, history of T2DM and aetiology of CPE. Density of BEC, SBN and LC were compared between the two groups. RESULTS: The healing time in days for CPE with different aetiologies in the T2DM and control groups were as follows: dry eye (21.56 ± 2.41; 7.00 ± 2.19; P = 0.001); meibomian gland dysfunction (26.42 ± 6.04; 9.21 ± 2.55; P = 0.001); cataract extraction (38.00 ± 19.62; 25.83 ± 11.49; P = 0.043); drug induced (53.19 ± 18.83; 41.86 ± 23.87; P = 0.018) and exposure (38.25 ± 14.13; 29.00 ± 13.67; P = 0.026). LC density was 38.70 ± 9.65 cells/mm in the T2DM group comparedwith 25.53 ± 3.54 cells/mm in the controls (P = 0.001). SBN density was 11.76 ± 1.69 mm/mm in the T2DM group compared with 20.92 ± 1.43 mm/mm in the controls (P = 0.001). BEC density in the T2DM group was 4982 ± 1178 cells/mm compared with 5739 ± 394 cells/mm in the control group (P = 0.018). Age and duration of T2DM had no relationship with healing time (multiple linear regression, P = 0.618; P = 0.787). The density of LC in the T2DM group showed a negative correlation with SBN density (r = 0.350; R = 0.1225; P = 0.034). The density of SBN in the T2DM group showed a positive correlation with BEC density (r = 0.427; R = 0.1823; P = 0.008). The density of BEC in the T2DM group showed a negative correlation with healing time (r = 0.931; R = 0.8668; P = 0.001). CONCLUSIONS: Utilising IVCM, we have demonstrated increased LC and decreased SBN in patients with T2DM and CPE. Both may be related to lower BEC density and nuclei enhanced reflection. Furthermore, decreased BEC density may lead to delay in cornea epithelium healing in the T2DM group comparedwith controls. An immune-mediated response may play a role in delayed wound closure in patients with T2DM.
[Mh] Termos MeSH primário: Doenças da Córnea/patologia
Diabetes Mellitus Tipo 2/complicações
Epitélio Anterior/patologia
Microscopia Confocal/métodos
[Mh] Termos MeSH secundário: Contagem de Células
Doenças da Córnea/etiologia
Progressão da Doença
Feminino
Seres Humanos
Masculino
Meia-Idade
Estudos Prospectivos
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-017-0645-6


  3 / 4889 MEDLINE  
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[PMID]:29172876
[Au] Autor:Yin J; Jurkunas U
[Ad] Endereço:a Massachusetts Eye and Ear, Department of Ophthalmology , Harvard Medical School , Boston , MA , USA.
[Ti] Título:Limbal Stem Cell Transplantation and Complications.
[So] Source:Semin Ophthalmol;33(1):134-141, 2018.
[Is] ISSN:1744-5205
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Corneal epithelial stem cells are adult somatic stem cells located at the limbus and represent the ultimate source of transparent corneal epithelium. When these limbal stem cells become dysfunctional or deficient, limbal stem cell deficiency (LSCD) develops. LSCD is a major cause of corneal scarring and is particularly prevalent in chemical and thermal burns of the ocular surface. LSCD leads to conjunctivalization of the corneal surface, neovascularization, recurrent or persistent epithelial defects, ocular surface inflammation, and scarring that, in turn, lead to decreased vision, pain, and impaired quality of life. Several techniques have been reported for limbal stem cell transplantation (LSCT). We introduce the surgical techniques, examine the success rate, and discuss the postoperative complications of conjunctival limbal autograft (CLAU), cultivated limbal stem cell transplantation (CLET), simple limbal epithelial transplantation (SLET), and limbal allograft, including keratolimbal allografts (KLAL) and living-related conjunctival allograft (LR-CLAL).
[Mh] Termos MeSH primário: Túnica Conjuntiva/transplante
Doenças da Córnea/cirurgia
Transplante de Córnea/métodos
Epitélio Anterior/transplante
Complicações Pós-Operatórias
Transplante de Células-Tronco/métodos
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Túnica Conjuntiva/citologia
Seres Humanos
Transplante Autólogo
Acuidade Visual
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1080/08820538.2017.1353834


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[PMID]:28469996
[Au] Autor:Cendra MDM; Christodoulides M; Hossain P
[Ad] Endereço:Division of Clinical and Experimental Sciences, Department of Molecular Microbiology, Faculty of Medicine, University of SouthamptonSouthampton, UK.
[Ti] Título:Signaling Mediated by Toll- Receptor 5 Sensing of Flagellin Influences IL-1ß and IL-18 Production by Primary Fibroblasts Derived from the Human Cornea.
[So] Source:Front Cell Infect Microbiol;7:130, 2017.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:is the principal cause of bacterial keratitis worldwide and overstimulation of the innate immune system by this organism is believed to contribute significantly to sight loss. In the current study, we have used primary human corneal fibroblast (hCF) cells as an model of corneal infection to examine the role of flagellum and type three secretion system (TTSS) in inducing inflammasome-associated molecules that trigger IL-1ß and IL-18 production during the early stages of the infection. Our results show that infection stimulated the non-canonical pathway for IL-1ß and IL-18 expression and pathway stimulation was influenced predominantly by the flagellum. Both IL-1ß and IL-18 cytokines were expressed intracellularly during bacterial infection, but only the former was released and detected in the extracellular environment. We also investigated the signaling pathways in hCFs mediated by Toll- Receptor (TLR)4 and TLR5 sensing of , and our data show that the signal triggered by TLR5-flagellin sensing significantly contributed to IL-1ß and IL-18 cytokine production in our model. Our study suggests that IL-18 expression is wholly dependent on extracellular flagellin sensing by TLR5, whereas IL-1ß expression is also influenced by lipopolysacharide. Additionally, we demonstrate that IL-1ß and IL-18 production by hCFs can be triggered by both MyD88-dependent and -independent pathways. Overall, our study provides a rationale for the development of targeted therapies, by proposing an inhibition of flagellin-PRR-signaling interactions, in order to ameliorate the inflammatory response characteristic of keratitis.
[Mh] Termos MeSH primário: Epitélio Anterior/efeitos dos fármacos
Fibroblastos/metabolismo
Flagelina/farmacologia
Interleucina-18/metabolismo
Interleucina-1beta/metabolismo
Pseudomonas aeruginosa/química
Transdução de Sinais/imunologia
Receptor 5 Toll-Like/metabolismo
[Mh] Termos MeSH secundário: Adesinas Bacterianas
Proteínas de Bactérias/genética
Proteínas de Bactérias/farmacologia
Células Cultivadas
Citocinas/metabolismo
Epitélio Anterior/imunologia
Flagelina/genética
Flagelina/imunologia
Flagelina/isolamento & purificação
Seres Humanos
Inflamassomos/metabolismo
Lipopolissacarídeos/imunologia
Pseudomonas aeruginosa/patogenicidade
Proteínas Recombinantes
Receptor 4 Toll-Like/metabolismo
Sistemas de Secreção Tipo III/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adhesins, Bacterial); 0 (Bacterial Proteins); 0 (Cytokines); 0 (FlgK protein, Bacteria); 0 (Inflammasomes); 0 (Interleukin-18); 0 (Interleukin-1beta); 0 (Lipopolysaccharides); 0 (Recombinant Proteins); 0 (TLR4 protein, human); 0 (TLR5 protein, human); 0 (Toll-Like Receptor 4); 0 (Toll-Like Receptor 5); 0 (Type III Secretion Systems); 12777-81-0 (Flagellin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2017.00130


  5 / 4889 MEDLINE  
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[PMID]:29331377
[Au] Autor:Jeong WY; Yoo HY; Kim CW
[Ad] Endereço:Department of Biotechnology, BK21 Plus Program, College of Life Sciences and Biotechnology, Korea University, 1-5, Anam Dong, Seongbuk-Gu, Seoul 136-701, South Korea.
[Ti] Título:ß-cellulin promotes the proliferation of corneal epithelial stem cells through the phosphorylation of erk1/2.
[So] Source:Biochem Biophys Res Commun;496(2):359-366, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The proliferation of corneal epithelial stem cells (CESCs) is a very important process in the recovery of corneal wounds. Recent studies have shown that ß-cellulin (BC) is effective in the repair of other tissues. However, its mechanism of action in corneal wound healing is not yet clear. The purpose of this study was to investigate how BC accelerates wound healing of the cornea. Here, we confirmed that the proliferation of CESCs was induced at a specific concentration (0.2, 2 and 20 ng/mL) by treatment with BC. Markers associated with proliferation activity (ΔNp63, bmi-1, abcg2) were also upregulated. In vivo experiments showed that the corneal wound healing rate was increased in mice. We found that BC stimulates the phosphorylation of the erk1/2 signaling pathway, which is triggered during the recovery of mouse corneal wounds. However, the inhibition of erk1/2 phosphorylation delayed the recovery of mouse corneal wounds in an organ culture assay. According to these results, BC may be a potential treatment factor for corneal wound healing.
[Mh] Termos MeSH primário: Betacelulina/farmacologia
Células Epiteliais/efeitos dos fármacos
Epitélio Anterior/efeitos dos fármacos
Proteína Quinase 1 Ativada por Mitógeno/genética
Proteína Quinase 3 Ativada por Mitógeno/genética
Células-Tronco/efeitos dos fármacos
[Mh] Termos MeSH secundário: Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Animais
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células Epiteliais/metabolismo
Células Epiteliais/patologia
Epitélio Anterior/lesões
Epitélio Anterior/metabolismo
Regulação da Expressão Gênica
Camundongos
Camundongos Endogâmicos BALB C
Proteína Quinase 1 Ativada por Mitógeno/antagonistas & inibidores
Proteína Quinase 1 Ativada por Mitógeno/metabolismo
Proteína Quinase 3 Ativada por Mitógeno/antagonistas & inibidores
Proteína Quinase 3 Ativada por Mitógeno/metabolismo
Técnicas de Cultura de Órgãos
Fosfoproteínas/genética
Fosfoproteínas/metabolismo
Fosforilação/efeitos dos fármacos
Complexo Repressor Polycomb 1/genética
Complexo Repressor Polycomb 1/metabolismo
Inibidores de Proteínas Quinases/farmacologia
Proteínas Proto-Oncogênicas/genética
Proteínas Proto-Oncogênicas/metabolismo
Células-Tronco/metabolismo
Células-Tronco/patologia
Transativadores/genética
Transativadores/metabolismo
Cicatrização/efeitos dos fármacos
Cicatrização/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP Binding Cassette Transporter, Sub-Family G, Member 2); 0 (Abcg2 protein, mouse); 0 (Betacellulin); 0 (Bmi1 protein, mouse); 0 (Btc protein, mouse); 0 (Phosphoproteins); 0 (Protein Kinase Inhibitors); 0 (Proto-Oncogene Proteins); 0 (Trans-Activators); 0 (Trp63 protein, mouse); EC 2.3.2.27 (Polycomb Repressive Complex 1); EC 2.7.11.24 (Mapk1 protein, mouse); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180115
[St] Status:MEDLINE


  6 / 4889 MEDLINE  
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[PMID]:28460048
[Au] Autor:Kolar SS; Baidouri H; McDermott AM
[Ad] Endereço:The Ocular Surface Institute, University of Houston, College of Optometry, Houston, Texas, United States.
[Ti] Título:Role of Pattern Recognition Receptors in the Modulation of Antimicrobial Peptide Expression in the Corneal Epithelial Innate Response to F. solani.
[So] Source:Invest Ophthalmol Vis Sci;58(5):2463-2472, 2017 05 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: Fusarium solani (F. solani) keratitis is a potentially sight-threatening fungal infection of the cornea. Antimicrobial peptides (AMPs), such as human ß-defensins (hBDs) and cathelicidins, essential components of the immune system, likely have a protective role against F. solani keratitis. We examined the role of pattern recognition receptors (PRRs), Dectin-1, and TLR2 in F. solani-induced modulation of AMP expression in vitro. Methods: Human corneal epithelial cells (HCECs) were exposed to heat-inactivated F. solani or pathogen-associated molecular patterns (PAMPs) of F. solani (Zymosan or Zymosan Depleted) for 6, 12, or 24 hours following which AMP mRNA and protein levels were determined. Involvement of TLR2 and Dectin-1 was confirmed by using siRNA knock-down (TLR2 and Dectin-1) or chemical inhibitor BAY 61-3606 (Dectin-1). The functional significance of AMP upregulation was tested using culture supernatant from F. solani or PAMP-treated HCECs against F. solani in the presence of hBD2 or LL37 neutralizing antibody. Results: We confirm that HCECs express Dectin-1 and TLR2. HCECs demonstrated upregulation of AMPs hBD2 and cathelicidin LL37 following exposure to heat-inactivated F. solani or PAMPs. TLR2 and Dectin-1 knockdown and BAY 61-3606 treatment decreased AMP mRNA upregulation confirming PRR involvement. The culture supernatant from F. solani or PAMP-treated HCECs showed substantial killing of F. solani and hBD2 or LL37 neutralizing antibody significantly decreased this effect implicating involvement of these AMPs. Conclusions: These findings demonstrate that Dectin-1 and TLR2 have an important role in regulating F. solani-induced AMP expression in corneal epithelial cells.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/genética
Epitélio Anterior/metabolismo
Infecções Oculares Fúngicas/metabolismo
Fusariose/metabolismo
Regulação da Expressão Gênica
Ceratite/metabolismo
Receptores de Reconhecimento de Padrão/genética
[Mh] Termos MeSH secundário: Peptídeos Catiônicos Antimicrobianos/biossíntese
Células Cultivadas
Córnea/metabolismo
Córnea/patologia
Epitélio Anterior/microbiologia
Epitélio Anterior/patologia
Infecções Oculares Fúngicas/imunologia
Citometria de Fluxo
Fusariose/diagnóstico
Fusariose/microbiologia
Seres Humanos
Immunoblotting
Ceratite/diagnóstico
Ceratite/microbiologia
RNA/genética
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Receptores de Reconhecimento de Padrão/metabolismo
Transdução de Sinais
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (RNA, Messenger); 0 (Receptors, Pattern Recognition); 63231-63-0 (RNA)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180124
[Lr] Data última revisão:
180124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-20658


  7 / 4889 MEDLINE  
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[PMID]:29260198
[Au] Autor:Kang KB; Lawrence BD; Gao XR; Luo Y; Zhou Q; Liu A; Guaiquil VH; Rosenblatt MI
[Ad] Endereço:Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago, Chicago, Illinois, United States.
[Ti] Título:Micro- and Nanoscale Topographies on Silk Regulate Gene Expression of Human Corneal Epithelial Cells.
[So] Source:Invest Ophthalmol Vis Sci;58(14):6388-6398, 2017 Dec 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: Corneal basement membrane has topographical features that provide biophysical cues to direct cell adherence, migration, and proliferation. In this study, we hypothesize that varying topographic pitch created on silk films can alter epithelial cell morphology, adhesion, and the genetic expression involved in cytoskeletal dynamics-related pathways. Methods: Silicon wafers with parallel ridge widths of 2000, 1000, and 800 nm were produced and used to pattern silk films via soft lithography. Human corneal epithelial cells were cultured onto silk. After 72 hours of incubation, images were taken to study cell morphology and alignment. Cytoskeletal structures were studied by immunofluorescent staining. RNA was collected from cultured cells to perform RNA-Seq transcriptome analysis using the Illumina Hiseq 2500 sequencing system. Differentially expressed genes were identified using DNAstar Qseq then verified using quantitative real-time PCR. These genes were used to perform pathway analyses using Ingenuity Pathways Analysis. Results: Primary human corneal epithelial cell alignment to the surface pattern was the greatest on 1000-nm features. Fluorescent microscopy of f-actin staining showed cell cytoskeleton alignment either in parallel (2000 nm) or perpendicular (1000 and 800 nm) to the long feature axis. Z-stack projection of vinculin staining indicated increased focal adhesion formation localized on the cellular basal surface. RNA-seq analysis revealed differentially expressed genes involved in actin organization, integrin signaling, and focal adhesion kinase signaling (-log (P)>5). Conclusions: Patterned silk film substrates may serve as a scaffold and provide biophysical cues to corneal epithelial cells that change their gene expression, alter cellular adherence, morphology, and may offer a promising customizable material for use in ocular surface repair.
[Mh] Termos MeSH primário: Células Epiteliais/citologia
Epitélio Anterior/citologia
Regulação da Expressão Gênica/efeitos dos fármacos
Seda/farmacologia
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Adesão Celular/efeitos dos fármacos
Células Cultivadas
Citoesqueleto/efeitos dos fármacos
Células Epiteliais/ultraestrutura
Perfilação da Expressão Gênica
Seres Humanos
Tecidos Suporte
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Silk)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22213


  8 / 4889 MEDLINE  
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[PMID]:29217022
[Au] Autor:Termote K; Schendel S; Moloney G; Holland SP; Lange AP
[Ad] Endereço:Cornea Unit, Department of Ophthalmology, Eye Care Center, University of British Columbia, Vancouver, B.C; Cornea, Cataract and Refractive Surgery Unit, Department of Ophthalmology, University Hospital Brussels, Brussels, Belgium.
[Ti] Título:Focal limbal stem cell deficiency associated with soft contact lens wear.
[So] Source:Can J Ophthalmol;52(6):552-558, 2017 Dec.
[Is] ISSN:1715-3360
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The aim of this study is to summarize the clinical characteristics of patients with contact lens-associated focal limbal stem cell deficiency (FLSCD) from a tertiary corneal referral centre. DESIGN: Retrospective, observational case series in a tertiary care centre. METHODS: Patients with contact lens-associated FLCSD were identified in our database. Clinical data were retrieved by chart review. A questionnaire asking for contact lens brand, type, cleaning solution, and duration of contact lens wear was sent to the patients with telephone follow-up. Clinical features and recovery time were identified. RESULTS: Twenty-seven eyes of 17 patients were identified with superior corneal whorl-like patches of opaque epithelium, sometimes accompanied by neovascularization. Of the patients, 17/17 used soft contact lenses, with a mean wearing duration of 11.4 hours per day. Patients had been wearing lenses for an average of 18.1 years. Silicone hydrogel lenses were noted in 12/17 cases. LSCD was superior in all 27 eyes, and all of them improved with contact lens wear cessation, preservative-free topical steroids, and preservative-free artificial tears. Visual acuity improved from 20/28 to 20/22 (p < 0.001). CONCLUSIONS: Contact lens-associated FLSCD typically presents in the superior cornea with whorl-like epithelial opacities advancing from the limbus. Conservative medical treatment is available and shows a high success rate after a slow recovery.
[Mh] Termos MeSH primário: Lentes de Contato Hidrofílicas/efeitos adversos
Doenças da Córnea/etiologia
Epitélio Anterior/patologia
Limbo da Córnea/patologia
Células-Tronco/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Lentes de Contato Hidrofílicas/utilização
Doenças da Córnea/terapia
Feminino
Seres Humanos
Masculino
Meia-Idade
Estudos Retrospectivos
Inquéritos e Questionários
Centros de Atenção Terciária
Fatores de Tempo
Acuidade Visual/fisiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


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[PMID]:29217021
[Au] Autor:Ross M; Deschênes J
[Ad] Endereço:Department of Ophthalmology, McGill University Health Center, McGill University, Montreal, Que.. Electronic address: michael.ross4@mail.mcgill.ca.
[Ti] Título:Practice patterns in the interdisciplinary management of corneal abrasions.
[So] Source:Can J Ophthalmol;52(6):548-551, 2017 Dec.
[Is] ISSN:1715-3360
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To characterize the treatment and follow-up patterns of corneal abrasions at an academic health centre. METHODS: This is a retrospective review of 90 cases of corneal abrasions over a 2-year period at a tertiary care academic hospital, of which 75 were seen by the ophthalmology department. All consultations primarily for corneal abrasion, as determined by the emergency department physician, were included in the study. Information on treatment regimen, corneal findings by emergency and ophthalmology physicians, time between follow-ups, and final outcomes was collected. RESULTS: Seventy-five patients were seen by ophthalmology a median of 1 day after the emergency room visit. Twenty-five of these patients did not arrive for their subsequent follow-up appointment. Twenty-two of the abrasions were healed by the time of the ophthalmology examination, 51 patients had unhealed corneal abrasions, and 2 had corneal ulcers. Management was changed in 29 of the patients by ophthalmology. The most common management changes were hypertonic saline ointment for prophylaxis or treatment of recurrent erosion syndrome, followed by bandage contact lenses for comfort. CONCLUSIONS: Corneal abrasions are a common condition, and practice patterns for follow-up vary widely. Although the vast majority of patients do very well and likely would heal on their own without ophthalmology referral, it seems reasonable that patients with corneal abrasions are assessed once by an ophthalmologist immediately or possibly up to 1-2 days after the initial emergency visit, depending on the individual patient circumstances.
[Mh] Termos MeSH primário: Lesões da Córnea/terapia
Epitélio Anterior/lesões
Oftalmologistas/estatística & dados numéricos
Equipe de Assistência ao Paciente
Padrões de Prática Médica/estatística & dados numéricos
[Mh] Termos MeSH secundário: Centros Médicos Acadêmicos
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Bandagens
Lentes de Contato
Serviço Hospitalar de Emergência
Feminino
Seres Humanos
Masculino
Meia-Idade
Pomadas
Equipe de Assistência ao Paciente/estatística & dados numéricos
Estudos Retrospectivos
Solução Salina Hipertônica/administração & dosagem
Centros de Atenção Terciária
Cicatrização
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Ointments); 0 (Saline Solution, Hypertonic)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


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[PMID]:29204644
[Au] Autor:Zhang X; Lin X; Liu Z; Wu Y; Yang Y; Ouyang W; Li W; Liu Z
[Ad] Endereço:Eye Institute of Xiamen University & Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.
[Ti] Título:Topical Application of Mizoribine Suppresses CD4+ T-cell-Mediated Pathogenesis in Murine Dry Eye.
[So] Source:Invest Ophthalmol Vis Sci;58(14):6056-6064, 2017 Dec 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: We investigate the effect of topical application of mizoribine (MZR) eye drops on CD4+ T-cell-mediated immunity and epithelial damage in ocular surface of dry eye disease (DED). Methods: Topical application of MZR or vehicle eye drops was performed in mice subjected to desiccating stress (DS). The phenol red cotton test was used to measure tear production, and Oregon-green-dextran (OGD) staining was performed to assess corneal epithelial barrier function. PAS staining was used to quantify conjunctival goblet cells. Immunofluorescent staining and quantitative (q) RT-PCR were used to assess the expression of matrix metalloproteinase (MMP)-9 in corneal epithelium. qRT-PCR and ELISA were used to assess the production of TNF-α and IL-1ß in conjunctiva. Apoptosis in ocular surface was assessed by TUNEL and activation of caspase-8. CD4+ T-cell-mediated immunity was evaluated by CD4 immunohistochemistry and production of T helper cytokines, including IFN-γ, IL-13, and IL-17A in conjunctiva. Results: Compared to vehicle control mice, topical MZR-treated mice showed increased tear production, decreased goblet cell loss, and improved corneal barrier function. Topical application of MZR suppressed the expression of MMP-9 in corneal epithelium and apoptosis in ocular surface, while it had no obvious effect on production of TNF-α and IL-1ß in conjunctiva. Topical application of MZR decreased CD4+ T cells infiltration, with decreased production of IFN-γ and IL-17A, and increased production of IL-13 in conjunctiva. Conclusions: Topical application of MZR could alleviate epithelial damage and CD4+ T-cell-mediated immunity in ocular surface of DED.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Síndromes do Olho Seco/tratamento farmacológico
Imunidade Celular/efeitos dos fármacos
Ribonucleosídeos/administração & dosagem
[Mh] Termos MeSH secundário: Administração Oftálmica
Animais
Apoptose
Linfócitos T CD4-Positivos/efeitos dos fármacos
Modelos Animais de Doenças
Síndromes do Olho Seco/imunologia
Síndromes do Olho Seco/patologia
Epitélio Anterior/efeitos dos fármacos
Epitélio Anterior/metabolismo
Epitélio Anterior/patologia
Feminino
Imuno-Histoquímica
Imunossupressores/administração & dosagem
Metaloproteinase 9 da Matriz/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Soluções Oftálmicas/administração & dosagem
Lágrimas/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunosuppressive Agents); 0 (Ophthalmic Solutions); 0 (Ribonucleosides); 4JR41A10VP (mizoribine); EC 3.4.24.35 (Matrix Metalloproteinase 9)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22852



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