Base de dados : MEDLINE
Pesquisa : A09.371.463 [Categoria DeCS]
Referências encontradas : 6172 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 618 ir para página                         

  1 / 6172 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29506497
[Au] Autor:Park SJ; Noh JH; Park KB; Jang SY; Lee JW
[Ad] Endereço:College of medicine, Soonchunhyang University, 204-ho, 31 Soonchunhyang-6-gil, Dongnam-gu, Cheonan, 31151, Choongcheongnam-do, South Korea.
[Ti] Título:A novel surgical technique for punctal stenosis: placement of three interrupted sutures after rectangular three-snip punctoplasty.
[So] Source:BMC Ophthalmol;18(1):70, 2018 Mar 05.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We developed a novel surgical technique to treat punctal stenosis involving the placement of three interrupted sutures after rectangular three-snip punctoplasty (TSP). METHODS: Retrospective chart review of forty-eight eyes of 44 patients who underwent rectangular TSP with three interrupted sutures was performed. We investigated whether anatomical recurrences (re-stenosis) occurred during the follow-up period. The subjective symptoms of patients were surveyed. RESULTS: The mean patient age was 64.1 years, and the mean follow-up time was 17.4 months. The placement of three interrupted sutures after rectangular TSP afforded satisfactory outcomes. Regarding subjective symptoms, 91.7% of the eyes (44/48) were reported as improved. Among 4 eyes determined as symptomatic failure, anatomical recurrence (re-stenosis of the punctum) was observed in only one eye. The other three (6.25%, 3/48 eyes) showed functional nasolacrimal obstruction, namely epiphora with patent tear duct. CONCLUSIONS: Placement of three interrupted sutures after rectangular TSP to treat punctal stenosis showed promising results. Notably anatomical success rate was about 98%. Further comparisons between the novel surgical technique and conventional techniques are required.
[Mh] Termos MeSH primário: Doenças Palpebrais/cirurgia
Aparelho Lacrimal/cirurgia
Obstrução dos Ductos Lacrimais/terapia
Procedimentos Cirúrgicos Oftalmológicos
Técnicas de Sutura
[Mh] Termos MeSH secundário: Feminino
Seguimentos
Seres Humanos
Masculino
Meia-Idade
Nylons
Estudos Retrospectivos
Suturas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nylons)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180307
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0733-2


  2 / 6172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28460477
[Au] Autor:Park E; Kim D; Lee SM; Jun HS
[Ad] Endereço:Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, Republic of Korea.
[Ti] Título:Inhibition of lysophosphatidic acid receptor ameliorates Sjögren's syndrome in NOD mice.
[So] Source:Oncotarget;8(16):27240-27251, 2017 Apr 18.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lysophosphatidic acid (LPA), a bioactive lysophospholipid, is involved in the pathogenesis of chronic inflammatory and autoimmune diseases. In this study, we investigated the role of LPA/LPA receptor (LPAR) signaling in the pathogenesis of Sjögren's syndrome (SS). We found that autotaxin, an LPA producing enzyme, and LPAR1 and LPAR3 mRNA, and IL-17 mRNA were highly expressed in the exocrine glands of 20-week-old nonobese diabetic (NOD) mice, which show SS symptoms at this age, as compared with non-symptomatic 8-week-old NOD mice. In an adoptive transfer model using NOD lymphocytes, treatment with Ki16425, an LPAR1/3 antagonist, restored tear and saliva secretion and decreased symptoms of SS compared with the vehicle-treated group. IL-17 levels in serum and lacrimal glands were also significantly reduced by Ki16425 in recipient mice. In addition, Ki16425 treatment of 20-week-old NOD mice, which spontaneously developed SS, restored saliva volume. Treatment of NOD splenocytes with LPA induced the expression of IL-17 in a dose-dependent manner, and Ki16425 inhibited this increase. LPA stimulated the activation of ROCK2 and p38 MAPK; and inhibition of ROCK2 or p38 MAPK suppressed LPA-induced IL-17 expression. Our data suggest that LPAR signaling stimulates SS development by induction of IL-17 production via ROCK and p38 MAPK pathways. Thus, LPAR inhibition could be a possible therapeutic strategy for SS.
[Mh] Termos MeSH primário: Receptores de Ácidos Lisofosfatídicos/antagonistas & inibidores
Receptores de Ácidos Lisofosfatídicos/metabolismo
Síndrome de Sjogren/metabolismo
[Mh] Termos MeSH secundário: Animais
Autoanticorpos/sangue
Autoanticorpos/imunologia
Citocinas/genética
Citocinas/metabolismo
Modelos Animais de Doenças
Feminino
Expressão Gênica
Imunoterapia Adotiva
Mediadores da Inflamação/metabolismo
Interleucina-17/sangue
Interleucina-17/metabolismo
Isoxazóis/farmacologia
Aparelho Lacrimal/imunologia
Aparelho Lacrimal/metabolismo
Aparelho Lacrimal/patologia
Masculino
Camundongos
Camundongos Endogâmicos NOD
Diester Fosfórico Hidrolases/genética
Diester Fosfórico Hidrolases/metabolismo
Propionatos/farmacologia
Receptores de Ácidos Lisofosfatídicos/genética
Saliva/metabolismo
Transdução de Sinais/efeitos dos fármacos
Síndrome de Sjogren/genética
Síndrome de Sjogren/imunologia
Síndrome de Sjogren/terapia
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
Quinases Associadas a rho/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3-(4-(4-((1-(2-chlorophenyl)ethoxy)carbonyl amino)-3-methyl-5-isoxazolyl) benzylsulfanyl) propanoic acid); 0 (Autoantibodies); 0 (Cytokines); 0 (Inflammation Mediators); 0 (Interleukin-17); 0 (Isoxazoles); 0 (Propionates); 0 (Receptors, Lysophosphatidic Acid); EC 2.7.11.1 (Rock2 protein, mouse); EC 2.7.11.1 (rho-Associated Kinases); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.1.4.39 (alkylglycerophosphoethanolamine phosphodiesterase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15916


  3 / 6172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29303941
[Au] Autor:Ali MJ; Gupta S; Patel A; Naik MN
[Ti] Título:Lacrimal Drainage Anomalies in Fraser Syndrome.
[So] Source:Ophthal Plast Reconstr Surg;34(1):92-93, 2018 Jan/Feb.
[Is] ISSN:1537-2677
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Dacriocistorinostomia/métodos
Síndrome de Fraser/complicações
Doenças do Aparelho Lacrimal/etiologia
Aparelho Lacrimal/diagnóstico por imagem
[Mh] Termos MeSH secundário: Criança
Síndrome de Fraser/diagnóstico
Seres Humanos
Aparelho Lacrimal/cirurgia
Doenças do Aparelho Lacrimal/diagnóstico
Doenças do Aparelho Lacrimal/cirurgia
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1097/IOP.0000000000001026


  4 / 6172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29309109
[Au] Autor:Bozic K; Glisic B; Radic-Tasic O; Knezevic B
[Ti] Título:Case report of Mikulicz's disease: A modern concept of an old entity.
[So] Source:Vojnosanit Pregl;73(4):393-6, 2016 Apr.
[Is] ISSN:0042-8450
[Cp] País de publicação:Serbia
[La] Idioma:eng
[Ab] Resumo:Introduction: Modern knowlegde defines Mikulicz´s disease as a part of immunoglobulin G4-related disease. The main feature is the presence of lymphoplasmacytic infiltrates, immunoglobulin G4 plasma cells positivity, distinctive storiform fibrosis and moderate eosinophilia. Case Report: A 59-years old male presented with a mild keratoconjuctivitis sicca and enlarged lacrimal and salivary glands during the last two years. Althought clinical presentation of the patient was typical, earlier testing did not pinpoint Mikulicz ´s disease. By typical clinical presentation, elevated serum immunoglobulin G4 level and histopathological finding of lacrimal glands tissue we diagnosed Mikulicz´s disease successfully treated with corticosteroid therapy. Conclusion: We reported the first case of IgG4-related Mikulicz´s disease in Serbia. Our report highlights IgG4-related Mikulicz` s disease as an important differential diagnosis with Sjögren`s syndrome and lymphoproliferative disease in rheumatological practice.
[Mh] Termos MeSH primário: Doença de Mikulicz/diagnóstico
[Mh] Termos MeSH secundário: Diagnóstico Diferencial
Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina G/metabolismo
Imuno-Histoquímica
Aparelho Lacrimal/imunologia
Transtornos Linfoproliferativos/diagnóstico
Masculino
Meia-Idade
Doença de Mikulicz/imunologia
Plasmócitos/imunologia
Sérvia
Síndrome de Sjogren/diagnóstico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin G)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE
[do] DOI:10.2298/VSP150118120B


  5 / 6172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27777501
[Au] Autor:Hawley D; Aluri H; Armaos H; Kim G; Kublin C; Zoukhri D
[Ad] Endereço:Department of Comprehensive Care, Tufts University School of Dental Medicine, Boston, MA.
[Ti] Título:Human postmortem lacrimal and submandibular glands stored in RNA are suitable for molecular, biochemical, and cell biological studies.
[So] Source:Mol Vis;22:1221-1228, 2016.
[Is] ISSN:1090-0535
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Gene expression and protein analysis studies require high-quality human tissue which is a challenge and difficult to obtain through live human biopsies. Human postmortem lacrimal gland (LG) and submandibular gland (SMG) tissues have the potential to provide an invaluable source for studying the mechanisms involved in LG and SMG dysfunction. Therefore, we aimed to test the suitability of post-mortem LG and SMG for molecular, biochemical, and cell biological studies. METHODS: LG and SMG tissue from healthy donors was collected and immediately placed in RNA solution and then shipped overnight at 4 °C. After receipt, each gland was divided into three pieces for RNA, protein, and histological analysis, respectively. Total RNA isolated from each LG and SMG was analyzed for RNA integrity using an Agilent Bioanalyzer and reverse transcription-PCR (RT-PCR). For histology, tissues were embedded in paraffin and stained with hematoxylin and eosin. For protein analysis, lysates were prepared and processed for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting. RESULTS: When the LG and SMG samples were preserved in RNA , the RNA integrity number (RIN) values from the LG and SMG were >7.0 from all three donors, while the RNAs from tissue not preserved in RNA were of poorer quality. The gene and/or protein expression of E-cadherin, aquaporin 5, alpha-smooth muscle actin (α-SMA), ß-actin, and GAPDH was preserved in all samples. In addition, histological analyses showed normal tubuloacinar structures of all glands with serous and mucous producing acini within lobules interspersed with adipose fat. CONCLUSIONS: In this study, we determined that RNA, protein, and histological sections obtained from postmortem human LG and SMG tissue preserved in RNA were of high quality. This would provide a viable source of human LG and SMG tissue suitable for studies of diseases that affect these glands, such as Sjögren's syndrome.
[Mh] Termos MeSH primário: Criopreservação
Proteínas do Olho/isolamento & purificação
Aparelho Lacrimal/química
Soluções para Preservação de Órgãos
Preservação de Órgãos
RNA/isolamento & purificação
Glândula Submandibular/química
[Mh] Termos MeSH secundário: Actinas/metabolismo
Idoso de 80 Anos ou mais
Aquaporina 5/metabolismo
Autopsia
Western Blotting
Caderinas/metabolismo
Eletroforese em Gel de Poliacrilamida
Proteínas do Olho/metabolismo
Feminino
Gliceraldeído 3-Fosfato Desidrogenase (NADP+)/metabolismo
Seres Humanos
Aparelho Lacrimal/metabolismo
Meia-Idade
RNA/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Glândula Submandibular/metabolismo
Doadores de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (ACTA2 protein, human); 0 (AQP5 protein, human); 0 (Actins); 0 (Aquaporin 5); 0 (CDH1 protein, human); 0 (Cadherins); 0 (Eye Proteins); 0 (Organ Preservation Solutions); 63231-63-0 (RNA); EC 1.2.1.9 (Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+))
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  6 / 6172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29217030
[Au] Autor:Abd El Ghafar AS; El-Kannishy AM; Elwan MM; Sabry D; Kishk HM; Elhefney EM
[Ad] Endereço:Ophthalmology Department, Ophthalmic Center, Mansoura University, Mansoura, Egypt.. Electronic address: aiman_eg_123@yahoo.com.
[Ti] Título:Perforated punctal plugs with adjuvant use of mitomycin-C in management of acquired external punctal stenosis grades 0 and 1.
[So] Source:Can J Ophthalmol;52(6):606-610, 2017 Dec.
[Is] ISSN:1715-3360
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To evaluate the use of perforated punctal plugs with adjuvant application of mitomycin-C in the management of acquired external punctal stenosis grades 0 and 1. METHODS: This is a prospective interventional case series including 30 eyes of 30 patients with acquired external punctal stenosis of the lower punctum of grades 0 and 1 presenting with epiphora. They were examined to exclude other causes of lacrimation and epiphora: Slit-lamp assessment of the stenosed punctum and grading was done, probing was done to exclude associated canalicular stenosis, and syringing was done to exclude nasolacrimal duct obstruction. For all cases, dilatation was done, followed by application of mitomycin-C, and perforated punctal plugs were then inserted. Plugs were removed after 6 months, and anatomical and functional success were assessed and followed during and for 6 months after removal of the plugs. RESULTS: The study included 30 patients with acquired external punctal stenosis: 9 males (30%) and 21 females (70%) with a mean age of 62.83 ± 8.3 years. Punctal stenosis grade 0 (no punctum) occurred in 9 cases (30%), and grade 1 (the punctum is covered by a membrane) occurred in 21 cases (70%). Six months after removal of the plugs, epiphora improved in 93.3% of patients, and fluorescein dye disappearance test results improved in 90% of patients; 6.7% of cases had early extrusion of the plug, and 3.3% had granuloma formation. CONCLUSIONS: Application of perforated punctal plugs combined with mitomycin-C is a simple and efficient technique in the management of tight acquired external punctal stenosis.
[Mh] Termos MeSH primário: Alquilantes/administração & dosagem
Pálpebras/patologia
Aparelho Lacrimal/patologia
Obstrução dos Ductos Lacrimais/terapia
Mitomicina/administração & dosagem
Plug Lacrimal
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Terapia Combinada
Desenho de Equipamento
Pálpebras/efeitos dos fármacos
Feminino
Corantes Fluorescentes/administração & dosagem
Seres Humanos
Obstrução dos Ductos Lacrimais/classificação
Obstrução dos Ductos Lacrimais/patologia
Masculino
Meia-Idade
Estudos Prospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkylating Agents); 0 (Fluorescent Dyes); 50SG953SK6 (Mitomycin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


  7 / 6172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29183042
[Au] Autor:Garreis F; Jahn J; Wild K; Abrar DB; Schicht M; Schröder JM; Paulsen F
[Ad] Endereço:Department of Anatomy II, Friedrich Alexander University Erlangen-Nürnberg (FAU), Erlangen, Germany.
[Ti] Título:Expression and Regulation of S100 Fused-Type Protein Hornerin at the Ocular Surface and Lacrimal Apparatus.
[So] Source:Invest Ophthalmol Vis Sci;58(13):5968-5977, 2017 Nov 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: The S100 fused-type proteins hornerin (HRNR) and filaggrin-2 (FLG2) are members of the epidermal differentiation complex, which is involved in terminal differentiation of keratinocytes via cornification as well as maintenance of the epidermal antimicrobial barrier. We investigated the expression and possible regulation of HRNR and FLG2 at the ocular surface and in the lacrimal apparatus. Methods: Tissues of the lacrimal apparatus and ocular surface were analyzed systematically by means of RT-PCR, immunohistochemistry, and immuntransmission electron microscopy (iTEM) for their ability to express and produce HRNR and FLG2. In addition, inducibility and regulation of HRNR were studied in cultivated human corneal (HCE), conjunctival (HCjE), as well as meibomian gland (HMGEC) epithelial cell line by real-time RT-PCR. Results: RT-PCR, immunohistochemistry, and iTEM revealed constitutive expression of HRNR in the epithelium of cornea, conjunctiva, nasolacrimal ducts, and acinus cells of lacrimal and meibomian glands. HRNR also was detected in tears of healthy volunteers. No expression of FLG2 could be detected in tissue samples of the ocular surface and lacrimal apparatus. Real-time RT-PCR revealed a decreased HRNR gene expression after challenge with proinflammatory cytokines and supernatants of Escherichia coli and Pseudomonas aeroginosa in HCE cells, whereas HCjE cells revealed no changes. In HMGECs serum-induced differentiation and application of all-trans retinoic acid significantly increased HRNR gene expression. Conclusions: The data suggest that HRNR, but not FLG2, is a component of the ocular surface and lacrimal apparatus, including meibomian glands. HRNR seems to contribute to the maintenance of the epithelial barrier at the ocular surface and, thus, also may be involved in ocular surface diseases.
[Mh] Termos MeSH primário: Túnica Conjuntiva/metabolismo
Córnea/metabolismo
Aparelho Lacrimal/metabolismo
Glândulas Tarsais/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Cadáver
Proteínas de Ligação ao Cálcio
Feminino
Regulação da Expressão Gênica
Seres Humanos
Imuno-Histoquímica
Proteínas de Filamentos Intermediários
Masculino
Microscopia Eletrônica
Meia-Idade
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Proteínas S100/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium-Binding Proteins); 0 (HRNR protein, human); 0 (Intermediate Filament Proteins); 0 (S100 Proteins); 0 (filaggrin-2 protein, human)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22637


  8 / 6172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29079859
[Au] Autor:Massie I; Spaniol K; Barbian A; Poschmann G; Stühler K; Geerling G; Metzger M; Mertsch S; Schrader S
[Ad] Endereço:Laboratory of Experimental Ophthalmology, University Clinic Düsseldorf, Düsseldorf, Germany.
[Ti] Título:Evaluation of Decellularized Porcine Jejunum as a Matrix for Lacrimal Gland Reconstruction In Vitro for Treatment of Dry Eye Syndrome.
[So] Source:Invest Ophthalmol Vis Sci;58(12):5564-5574, 2017 Oct 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: Dry eye syndrome (DES) can cause blindness in severe cases, but mainly palliative treatments exist. A tissue-engineered lacrimal gland (LG) could provide a curative treatment. We aimed to evaluate decellularized porcine jejunum (SIS-Muc) as a scaffold for porcine LG epithelial cells. Methods: To evaluate SIS-Muc as a potential scaffold, basement membrane proteins in SIS-Muc and native LG were compared (immunohistochemistry [IHC]). Porcine LG epithelial cells cultured on plastic were characterized (immunocytochemistry), and their culture supernatant was compared with porcine tears (proteomics). Epithelial cells were then seeded onto SIS-Muc in either a static (cell crown) or dynamic culture (within a perfusion chamber) and metabolic (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) and secretory capacities (ß-hexosaminidase assay), protein expression (IHC), and ultrastructure transmission electron microscopy (TEM) compared in each. Results: Collagen IV and laminin were found in both native LG and SIS-Muc. When cultured on plastic, LG epithelial cells expressed pan-cytokeratin, Rab3D, HexA, and produced mucins, but lysozyme and lactoferrin expression was nearly absent. Some porcine tear proteins (lipocalin-2 and lactoferrin) were found in LG epithelial cell culture supernatants. When LG cells were cultured on SIS-Muc, metabolic and ß-hexosaminidase activities were greater in dynamic cultures than static cultures (P < 0.05). In both static and dynamic cultures, cells expressed pan-cytokeratin, Rab3D, lysozyme, and lactoferrin and produced mucins, and TEM revealed cell polarization at the apical surface and cell-cell and cell-scaffold contacts. Conclusions: SIS-Muc is a suitable scaffold for LG cell expansion and may be useful toward reconstruction of LG tissue to provide a curative treatment for DES. Dynamic culture enhances cell metabolic and functional activities.
[Mh] Termos MeSH primário: Síndromes do Olho Seco/cirurgia
Jejuno/transplante
Aparelho Lacrimal/cirurgia
Procedimentos Cirúrgicos Oftalmológicos/métodos
Procedimentos Cirúrgicos Reconstrutivos/métodos
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Animais
Proliferação Celular
Células Cultivadas
Modelos Animais de Doenças
Síndromes do Olho Seco/diagnóstico
Ensaio de Imunoadsorção Enzimática
Imuno-Histoquímica
Jejuno/ultraestrutura
Aparelho Lacrimal/ultraestrutura
Microscopia Eletrônica de Transmissão
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171029
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-20759


  9 / 6172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29028795
[Au] Autor:Garg A; Bansal M; Gotoh N; Feng GS; Zhong J; Wang F; Kariminejad A; Brooks S; Zhang X
[Ad] Endereço:Departments of Ophthalmology, Pathology and Cell Biology, Columbia University, New York, NY, United States of America.
[Ti] Título:Alx4 relays sequential FGF signaling to induce lacrimal gland morphogenesis.
[So] Source:PLoS Genet;13(10):e1007047, 2017 Oct.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The sequential use of signaling pathways is essential for the guidance of pluripotent progenitors into diverse cell fates. Here, we show that Shp2 exclusively mediates FGF but not PDGF signaling in the neural crest to control lacrimal gland development. In addition to preventing p53-independent apoptosis and promoting the migration of Sox10-expressing neural crests, Shp2 is also required for expression of the homeodomain transcription factor Alx4, which directly controls Fgf10 expression in the periocular mesenchyme that is necessary for lacrimal gland induction. We show that Alx4 binds an Fgf10 intronic element conserved in terrestrial but not aquatic animals, underlying the evolutionary emergence of the lacrimal gland system in response to an airy environment. Inactivation of ALX4/Alx4 causes lacrimal gland aplasia in both human and mouse. These results reveal a key role of Alx4 in mediating FGF-Shp2-FGF signaling in the neural crest for lacrimal gland development.
[Mh] Termos MeSH primário: Fator 10 de Crescimento de Fibroblastos/genética
Proteínas de Homeodomínio/genética
Aparelho Lacrimal/crescimento & desenvolvimento
Morfogênese/genética
Crista Neural/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Diferenciação Celular/genética
Linhagem da Célula/genética
Regulação da Expressão Gênica no Desenvolvimento
Seres Humanos
Aparelho Lacrimal/metabolismo
Mesoderma/crescimento & desenvolvimento
Camundongos
Células-Tronco Pluripotentes/metabolismo
Ligação Proteica
Fatores de Transcrição SOXE/genética
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alx4 protein, mouse); 0 (Fgf10 protein, mouse); 0 (Fibroblast Growth Factor 10); 0 (Homeodomain Proteins); 0 (SOXE Transcription Factors); 0 (Sox10 protein, mouse)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007047


  10 / 6172 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28994268
[Au] Autor:Hur MC; Jin SW; Roh MS; Jeong WJ; Ryu WY; Kwon YH; Ahn HB
[Ad] Endereço:Department of Ophthalmology, Han Heart Hospital, Changwon, Korea.
[Ti] Título:Classification of Lacrimal Punctal Stenosis and Its Related Histopathological Feature in Patients with Epiphora.
[So] Source:Korean J Ophthalmol;31(5):375-382, 2017 Oct.
[Is] ISSN:2092-9382
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To evaluate the classification of punctal stenosis based on the shape of the external punctum, clinical characteristics and histopathologic features. METHODS: Patients who experienced tearing and were diagnosed with punctal stenosis were evaluated in this study. Punctal stenosis was classified according to the shape of the lower external punctum, which included membranous type, slit type, horseshoe type, and pinpoint type. Tear meniscus height, 2% fluorescein dye disappearance test and lacrimal pathway irrigation were measured or performed. For treatment, a punctal snip operation and silicone tube placement were performed, and the peripunctal histopathological findings were evaluated. RESULTS: Punctal stenosis was classified into four types: membranous type (17 eyes, 21.5%), slit type (11 eyes, 13.9%), horseshoe type (25 eyes, 31.6%), and pinpoint type (26 eyes, 32.9%). The tear meniscus was significantly higher, and the 2% fluorescein dye disappeared significantly more slowly in the punctal stenosis group. However, correlation of the tear meniscus height and 2% fluorescein dye disappearance test with the punctum shape was not statistically significant. A history of previous chemotherapy was significantly associated with the occurrence of punctal stenosis, especially the membranous type (p < 0.05). Histopathologic evaluation of the punctum showed differences between the punctum types. Pinpoint puncta exhibited a high density of muscle fibers, while they were faintly visible in the membranous type. CONCLUSIONS: Acquired punctal stenosis has various shapes, and the major types of stenotic puncta exhibited unique histopathologic features. Punctal stenosis and its pathophysiology may be related to multiple factors, such as age and systemic 5-fluorouracil chemotherapy history.
[Mh] Termos MeSH primário: Aparelho Lacrimal/patologia
Obstrução dos Ductos Lacrimais/classificação
[Mh] Termos MeSH secundário: Adulto
Idoso
Dacriocistorinostomia/métodos
Feminino
Seres Humanos
Obstrução dos Ductos Lacrimais/diagnóstico
Masculino
Meia-Idade
Estudos Prospectivos
Índice de Gravidade de Doença
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE
[do] DOI:10.3341/kjo.2016.0129



página 1 de 618 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde