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  1 / 62009 MEDLINE  
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[PMID]:29214788
[Au] Autor:Bae HW; Lee SY; Kim S; Park CK; Lee K; Kim CY; Seong GJ
[Ad] Endereço:Department of Ophthalmology, Severance Hospital, Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea.
[Ti] Título:Asymmetry of Peak Thicknesses between the Superior and Inferior Retinal Nerve Fiber Layers for Early Glaucoma Detection: A Simple Screening Method.
[So] Source:Yonsei Med J;59(1):135-140, 2018 Jan.
[Is] ISSN:1976-2437
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To assess whether the asymmetry in the peripapillary retinal nerve fiber layer (pRNFL) thickness between superior and inferior hemispheres on optical coherence tomography (OCT) is useful for early detection of glaucoma. MATERIALS AND METHODS: The patient population consisted of Training set (a total of 60 subjects with early glaucoma and 59 normal subjects) and Validation set (30 subjects with early glaucoma and 30 normal subjects). Two kinds of ratios were employed to measure the asymmetry between the superior and inferior pRNFL thickness using OCT. One was the ratio of the superior to inferior peak thicknesses (peak pRNFL thickness ratio; PTR), and the other was the ratio of the superior to inferior average thickness (average pRNFL thickness ratio; ATR). The diagnostic abilities of the PTR and ATR were compared to the color code classification in OCT. Using the optimal cut-off values of the PTR and ATR obtained from the Training set, the two ratios were independently validated for diagnostic capability. RESULTS: For the Training set, the sensitivities/specificities of the PTR, ATR, quadrants color code classification, and clock-hour color code classification were 81.7%/93.2%, 71.7%/74.6%, 75.0%/93.2%, and 75.0%/79.7%, respectively. The PTR showed a better diagnostic performance for early glaucoma detection than the ATR and the clock-hour color code classification in terms of areas under the receiver operating characteristic curves (AUCs) (0.898, 0.765, and 0.773, respectively). For the Validation set, the PTR also showed the best sensitivity and AUC. CONCLUSION: The PTR is a simple method with considerable diagnostic ability for early glaucoma detection. It can, therefore, be widely used as a new screening method for early glaucoma.
[Mh] Termos MeSH primário: Diagnóstico Precoce
Glaucoma/diagnóstico
Programas de Rastreamento/métodos
Fibras Nervosas/patologia
Retina/patologia
[Mh] Termos MeSH secundário: Área Sob a Curva
Cor
Feminino
Seres Humanos
Masculino
Meia-Idade
Curva ROC
Reprodutibilidade dos Testes
Células Ganglionares da Retina
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.3349/ymj.2018.59.1.135


  2 / 62009 MEDLINE  
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[PMID]:28466070
[Au] Autor:Sabbah S; Berg D; Papendorp C; Briggman KL; Berson DM
[Ad] Endereço:Department of Neuroscience, Brown University, Providence, RI 02912.
[Ti] Título:A Cre Mouse Line for Probing Irradiance- and Direction-Encoding Retinal Networks.
[So] Source:eNeuro;4(2), 2017 Mar-Apr.
[Is] ISSN:2373-2822
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell type-specific Cre driver lines have revolutionized the analysis of retinal cell types and circuits. We show that the transgenic mouse Rbp4-Cre selectively labels several retinal neuronal types relevant to the encoding of absolute light intensity (irradiance) and visual motion. In the ganglion cell layer (GCL), most marked cells are wide-field spiking polyaxonal amacrine cells (ACs) with sustained irradiance-encoding ON responses that persist during chemical synaptic blockade. Their arbors spread about 1 mm across the retina and are restricted to the inner half of the ON sublamina of the inner plexiform layer (IPL). There, they costratify with dendrites of M2 intrinsically photosensitive retinal ganglion cells (ipRGCs), to which they are tracer coupled. We propose that synaptically driven and intrinsic photocurrents of M2 cells pass through gap junctions to drive AC light responses. Also marked in this mouse are two types of RGCs. R-cells have a bistratified dendritic arbor, weak directional tuning, and irradiance-encoding ON responses. However, they also receive excitatory OFF input, revealed during ON-channel blockade. Serial blockface electron microscopic (SBEM) reconstruction confirms OFF bipolar input, and reveals that some OFF input derives from a novel type of OFF bipolar cell (BC). R-cells innervate specific layers of the dorsal lateral geniculate nucleus (dLGN) and superior colliculus (SC). The other marked RGC type (RDS) is bistratified, transient, and ON-OFF direction selective (DS). It apparently innervates the nucleus of the optic tract (NOT). The Rbp4-Cre mouse will be valuable for targeting these cell types for further study and for selectively manipulating them for circuit analysis.
[Mh] Termos MeSH primário: Rede Nervosa/fisiologia
Retina/fisiologia
Sinapses/fisiologia
Vias Visuais/fisiologia
[Mh] Termos MeSH secundário: Animais
Dendritos/metabolismo
Camundongos Transgênicos
Microscopia Eletrônica
Células Ganglionares da Retina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  3 / 62009 MEDLINE  
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[PMID]:29396367
[Au] Autor:Police A; Shankar VK; Narasimha Murthy S
[Ad] Endereço:Department of Pharmaceutics and Drug Delivery, University of Mississippi, MS 38677, USA.
[Ti] Título:RP-HPLC method for simultaneous estimation of vigabatrin, gamma-aminobutyric acid and taurine in biological samples.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1076:44-53, 2018 Feb 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Vigabatrin is used as first line drug in treatment of infantile spasms for its potential benefit overweighing risk of causing permanent peripheral visual field defects and retinal damage. Chronic administration of vigabatrin in rats has demonstrated these ocular events are result of GABA accumulation and depletion of taurine levels in retinal tissues. In vigabatrin clinical studies taurine plasma level is considered as biomarker for studying structure and function of retina. The analytical method is essential to monitor taurine levels along with vigabatrin and GABA. A RP-HPLC method has been developed and validated for simultaneous estimation of vigabatrin, GABA and taurine using surrogate matrix. Analytes were extracted from human plasma, rat plasma, retina and brain by simple protein precipitation method and derivatized by naphthalene 2, 3­dicarboxaldehyde to produce stable fluorescent active isoindole derivatives. The chromatographic analysis was performed on Zorbax Eclipse AAA column using gradient elution profile and eluent was monitored using fluorescence detector. A linear plot of calibration curve was observed in concentration range of 64.6 to 6458, 51.5 to 5150 and 62.5 to 6258 ng/mL for vigabatrin, GABA and taurine, respectively with r ≥ 0.997 for all analytes. The method was successfully applied for estimating levels of vigabatrin and its modulator effect on GABA and taurine levels in rat plasma, brain and retinal tissue. This RP-HPLC method can be applied in clinical and preclinical studies to explore the effect of taurine deficiency and to investigate novel approaches for alleviating vigabatrin induced ocular toxicity.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Taurina/análise
Vigabatrina/análise
Ácido gama-Aminobutírico/análise
[Mh] Termos MeSH secundário: Animais
Química Encefálica
Cromatografia de Fase Reversa/métodos
Seres Humanos
Modelos Lineares
Masculino
Ratos
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Retina/química
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
1EQV5MLY3D (Taurine); 56-12-2 (gamma-Aminobutyric Acid); GR120KRT6K (Vigabatrin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180204
[St] Status:MEDLINE


  4 / 62009 MEDLINE  
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[PMID]:29486754
[Au] Autor:Fusi-Rubiano W; Mukherjee C; Lane M; Tsaloumas MD; Glover N; Kidess A; Denniston AK; Palmer HE; Manna A; Morjaria R
[Ad] Endereço:Ophthalmology Department, Queen Elizabeth Hospital Birmingham, University Hospitals Birmingham NHSFT, Mindelsohn Way, Birmingham, B15 2TH, United Kingdom.
[Ti] Título:Treating Diabetic Macular Oedema (DMO): real world UK clinical outcomes for the 0.19mg Fluocinolone Acetonide intravitreal implant (Iluvien™) at 2 years.
[So] Source:BMC Ophthalmol;18(1):62, 2018 Feb 27.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: To compare visual function and structural improvements in pseudophakic eyes with diabetic macular oedema (DMO) treated with the 0.19mg Fluocinolone Acetonide (FAc) intravitreal implant (Iluvien ) in a 'real world' setting. METHODS: A single centre retrospective evaluation of patients with DMO unresponsive to conventional treatment treated with the FAc implant according to UK guidelines. Primary efficacy endpoint was best corrected visual acuity (BCVA); secondary endpoints included optical coherence tomography evaluations of the macula (a) central retinal and (b) peak macular thickness collected at annual time points. Primary safety endpoint was new rise in IOP >27mmHg or glaucoma surgery. Patients with <1 year follow-up were excluded. RESULTS: Twenty-nine eyes were included, with mean(SD) follow up of 792(270) days. Improvement in BCVA and reduction in macular oedema was noted at all timepoints. Mean improvement in BCVA from baseline was 6 ETDRS letters at year 1(n=29), 6.5L at year 2(n=22) and 11L at year 3(n=6). Mean central retinal thickness at baseline was 451 microns, 337 microns at year 1, 342 microns at year 2 and 314 microns at year 3. Two eyes required IOP-lowering drops post implant. Supplementary treatment for persistence or recurrence of DMO was necessary in 18 eyes over the total study period of 3 years with mean time to supplementary treatment being 12 months. CONCLUSIONS: Our evaluation of the 0.19mg FAc implant delivered in a real-world setting, provides additional evidence that it is effective and safe in the treatment of patients with DMO, and can provide sustained benefit for patients with previously refractory disease.
[Mh] Termos MeSH primário: Retinopatia Diabética/tratamento farmacológico
Fluocinolona Acetonida/administração & dosagem
Glucocorticoides/administração & dosagem
Edema Macular/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Retinopatia Diabética/patologia
Retinopatia Diabética/fisiopatologia
Implantes de Medicamento
Feminino
Seres Humanos
Injeções Intravítreas
Edema Macular/patologia
Edema Macular/fisiopatologia
Masculino
Meia-Idade
Retina/patologia
Estudos Retrospectivos
Reino Unido
Acuidade Visual/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drug Implants); 0 (Glucocorticoids); 0CD5FD6S2M (Fluocinolone Acetonide)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180301
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0726-1


  5 / 62009 MEDLINE  
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[PMID]:29482501
[Au] Autor:Ishizaki N; Kida T; Fukumoto M; Sato T; Oku H; Ikeda T
[Ad] Endereço:Department of Ophthalmology, Yao Tokushukai General Hospital, 1-17 Wakakusa-cho, Yao, Japan.
[Ti] Título:Development of macular retinoschisis long after the onset of retinal arterial occlusion (RAO): a retrospective study.
[So] Source:BMC Ophthalmol;18(1):59, 2018 Feb 27.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: To describe a retrospective study of macular retinoschisis that developed long after the onset of retinal artery occlusion (RAO) using optical coherence tomography (OCT). METHODS: We describe changes in macular findings and visual acuity (VA) of 29 patients (21 males and 8 females, mean age: 66.1 ± 16.9 years) with RAO (18 branch RAOs [BRAOs] and 11 central RAOs [CRAOs] who visited Osaka Medical College Hospital over an 8-year period based on a medical chart review. RESULTS: The mean VA (logMAR) increased from 1.06 ± 1.08 (CRAO: 2.04 ± 0.99; BRAO: 0.37 ± 0.40) at the first visit to 0.71 ± 0.87 (CRAO: 1.46 ± 0.86; BRAO: 0.18 ± 0.30) at the final visit. Macular OCT revealed swelling or hyper-reflectivity of the inner retina in the early phase of RAO and retinal thinning in the late phase. Among the 29 patients, two patients (a patient with BRAO and a patient with CRAO) developed macular retinoschisis about 1 year after RAO onset. The VA of the patient with BRAO was 20/300 at the first visit, and it improved to 20/25 two days after onset following eye massage and anterior chamber paracentesis. However, his VA worsened, declining from 20/25 to 20/50, and retinoschisis occurred 13 months after RAO onset. The patient with CRAO showed macular changes including small cystoids at the first follow-up visit more than 3 weeks after onset and developed retinoschisis 11 months after the first visit. In addition, two patients with BRAO and one patient with CRAO developed macular changes including small cystoids 3 weeks after onset, with the BRAO complicated by retinal vein occlusion. In the CRAO patient, the cystoid macular edema was resolved 1 month after the first visit. CONCLUSIONS: Macular retinoschisis is unusual, but a possible complication of RAO that can develop long after the onset of the occlusion, potentially resulting in renewed VA deterioration.
[Mh] Termos MeSH primário: Oclusão da Artéria Retiniana/complicações
Retinosquise/etiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Macula Lutea/diagnóstico por imagem
Macula Lutea/patologia
Edema Macular/diagnóstico por imagem
Edema Macular/etiologia
Edema Macular/patologia
Edema Macular/fisiopatologia
Masculino
Meia-Idade
Retina/patologia
Oclusão da Artéria Retiniana/diagnóstico por imagem
Retinosquise/diagnóstico por imagem
Retinosquise/patologia
Retinosquise/fisiopatologia
Estudos Retrospectivos
Tomografia de Coerência Óptica/métodos
Acuidade Visual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180228
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0730-5


  6 / 62009 MEDLINE  
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[PMID]:29482510
[Au] Autor:Zhao MH; Hu J; Li S; Wu Q; Lu P
[Ad] Endereço:Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.
[Ti] Título:P66Shc expression in diabetic rat retina.
[So] Source:BMC Ophthalmol;18(1):58, 2018 Feb 27.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: P66Shc is partially localised within the mitochondrial fraction. It is primarily related to the generation of mitochondrial reactive oxygen species and apoptosis. Based on previous studies, we hypothesize that in the retina, p66Shc may exist and affect the development of diabetic retinopathy. The purpose of this study was to investigate p66Shc expression in retinal in streptozotocin-induced diabetic (SD) rats, which may provide a pathway to study the pathogenesis of diabetic retinopathy. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) and western blot were used to detect retinal p66Shc mRNA and protein expression in SD rats, respectively. Immunohistochemical staining was applied to detect the location of rat retinal p66Shc expression. TUNEL assay was applied to detect the number of apoptotic cells. RESULTS: P66Shc expression was found in the retina of normal and diabetic rats, and the level of mRNA and protein expression increased with the progression of diabetes mellitus (DM). P66Shc expression was mainly located in the retinal ganglion cell layer and inner nuclear layer. Compared with the normal group, retinal cell tissue apoptosis rate in the D12w group was significantly increased. CONCLUSION: Rat retinal p66Shc expression was mainly in the ganglion cell layer and inner nuclear layer. As the degree of DM progressed, p66Shc expression gradually increased, and the number of apoptotic cells also increased.
[Mh] Termos MeSH primário: Diabetes Mellitus Experimental/metabolismo
Retinopatia Diabética/metabolismo
Retina/metabolismo
Proteína 1 de Transformação que Contém Domínio 2 de Homologia de Src/metabolismo
[Mh] Termos MeSH secundário: Animais
Western Blotting
Imuno-Histoquímica
Marcação In Situ das Extremidades Cortadas
Masculino
Ratos
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase em Tempo Real
Células Ganglionares da Retina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Shc1 protein, rat); 0 (Src Homology 2 Domain-Containing, Transforming Protein 1)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180228
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0724-3


  7 / 62009 MEDLINE  
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[PMID]:29384610
[Au] Autor:Dobias JJ; Papathomas TV; Sarwate A
[Ti] Título:Ponzo's Illusion in 3D: Perspective Gradients Dominate Differences in Retinal Size.
[So] Source:Multisens Res;29(4-5):421-38, 2016.
[Is] ISSN:2213-4794
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A common form of the Ponzo illusion involves two test probes of equal size, embedded in a planar linear perspective painting depicting a three-dimensional (3D) scene, where the probe perceived to be farther is judged to be larger than the probe perceived closer to the viewer. In this paper, the same perspective 3D scene was painted on three surfaces: (a) A 2D surface incongruent with the 3D painted scene (flat perspective). (b) A 3D surface with a geometry congruent with the 3D scene (proper perspective). (c) A 3D surface with an opposite depth arrangement to the 3D scene (reverse perspective). This last stimulus was bistable and could be perceived veridically, as it physically existed, or as a depth-inverting illusion. For all experiments, observers relied on perspective gradients to estimate the size of a test probe placed within the scene; objects placed in a 'far' position as defined by perspective cues were perceived to be larger regardless of their physical distance. Further, illusion strength was tied to retinal size; small retinal-size differences (Experiments 1 and 2) did not affect illusion strength, whereas larger retinal-size differences (Experiment 3) did play a minor role.
[Mh] Termos MeSH primário: Imagem Tridimensional
Ilusões Ópticas/fisiologia
Retina/fisiologia
Percepção Visual/fisiologia
[Mh] Termos MeSH secundário: Adolescente
Seres Humanos
Tempo de Reação
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180201
[St] Status:MEDLINE


  8 / 62009 MEDLINE  
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[PMID]:28457994
[Au] Autor:Kady N; Yan Y; Salazar T; Wang Q; Chakravarthy H; Huang C; Beli E; Navitskaya S; Grant M; Busik J
[Ad] Endereço:Department of Physiology, Michigan State University, East Lansing, MI, USA.
[Ti] Título:Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34 circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes.
[So] Source:J Clin Lipidol;11(3):694-703, 2017 May - Jun.
[Is] ISSN:1933-2874
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Diabetic retinopathy is a microvascular disease that results from retinal vascular degeneration and defective repair due to diabetes-induced endothelial progenitor dysfunction. OBJECTIVE: Understanding key molecular factors involved in vascular degeneration and repair is paramount for developing effective diabetic retinopathy treatment strategies. We propose that diabetes-induced activation of acid sphingomyelinase (ASM) plays essential role in retinal endothelial and CD34 circulating angiogenic cell (CAC) dysfunction in diabetes. METHODS: Human retinal endothelial cells (HRECs) isolated from control and diabetic donor tissue and human CD34 CACs from control and diabetic patients were used in this study. ASM messenger RNA and protein expression were assessed by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. To evaluate the effect of diabetes-induced ASM on HRECs and CD34 CACs function, tube formation, CAC incorporation into endothelial tubes, and diurnal release of CD34 CACs in diabetic individuals were determined. RESULTS: ASM expression level was significantly increased in HRECs isolated from diabetic compared with control donor tissue, as well as CD34 CACs and plasma of diabetic patients. A significant decrease in tube area was observed in HRECs from diabetic donors compared with control HRECs. The tube formation deficiency was associated with increased expression of ASM in diabetic HRECs. Moreover, diabetic CD34 CACs with high ASM showed defective incorporation into endothelial tubes. Diurnal release of CD34 CACs was disrupted with the rhythmicity lost in diabetic patients. CONCLUSION: Collectively, these findings support that diabetes-induced ASM upregulation has a marked detrimental effect on both retinal endothelial cells and CACs.
[Mh] Termos MeSH primário: Retinopatia Diabética/enzimologia
Células Endoteliais/metabolismo
Neovascularização Patológica/patologia
Retina/patologia
Vasos Retinianos/fisiopatologia
Esfingomielina Fosfodiesterase/metabolismo
[Mh] Termos MeSH secundário: Idoso
Antígenos CD34/metabolismo
Ritmo Circadiano
Retinopatia Diabética/sangue
Retinopatia Diabética/patologia
Retinopatia Diabética/fisiopatologia
Células Endoteliais/patologia
Feminino
Regulação Enzimológica da Expressão Gênica
Seres Humanos
Masculino
Meia-Idade
Vasos Retinianos/metabolismo
Esfingomielina Fosfodiesterase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD34); EC 3.1.4.- (acid sphingomyelinase-1); EC 3.1.4.12 (Sphingomyelin Phosphodiesterase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  9 / 62009 MEDLINE  
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[PMID]:29232606
[Au] Autor:Kotnala A; Senthilkumari S; Halder N; Kumar A; Velpandian T
[Ad] Endereço:Ocular Pharmacology & Pharmacy Division, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India.
[Ti] Título:Microwave assisted synthesis for A2E and development of LC-ESI-MS method for quantification of ocular bisretinoids in human retina.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1073:10-18, 2018 Jan 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To develop a microwave assisted method for the rapid synthesis of A2E and also to develop a method to quantify N-retinylidene-N-retinylethanolamine(A2E), all-trans retinal dimer (ATRD), A2-glycerophospho ethanolamine (A2GPE), dihydropyridine phosphatidyl ethanolamine (A2DHPE) and monofuran A2E (MFA2E) in age matched retina. METHODS: The development of microwave assisted synthesis of A2E, its purification and characterization for its utility in quantification in human retina. The semi-quantitative method development using LC-ESI-MS, LC-ESI-MS/MS and LC-APCI-MS/MS from pooled macula and peripheral retina for the bisretinoid analysis has been done. RESULTS: Maximum A2E conversion using microwave assisted process took place at 80°C for 45min with a yield of 55.01%. Highly sensitive and specific mass spectrometric method was developed using reverse phase C-18 separation with positive electrospray ionization and positive atmospheric phase chemical ionization of tandom mass spectrometry. A gradient mobile phase separation was achieved using water and methanol with 0.1% TFA. Multiple reaction monitoring acquisition for ESI and APCI was performed at ATRD m/z 551.2/522.2, A2GPE m/z 746.4/729.5, A2DHPEm/z 594.4/576.5, MFA2E m/z 608.2/591.2, A2E m/z 592.4/418.2. Method was validated using LC-ESI-SIM mode to determine selectivity, linearity, sensitivity, precision and accuracy. CONCLUSION: An attempt towards optimization of the synthetic procedure of A2E was made so as to reduce the lengthy reaction time without compromising the yield. Developed method was capable enough for the detection of low level of bisretinids in retina.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Micro-Ondas
Retina/química
Retinoides/análise
Retinoides/síntese química
Espectrometria de Massas por Ionização por Electrospray/métodos
[Mh] Termos MeSH secundário: Adulto
Idoso
Seres Humanos
Masculino
Meia-Idade
Retinoides/química
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (A2-E (N-retinylidene-N-retinylethanolamine)); 0 (Retinoids)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


  10 / 62009 MEDLINE  
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[PMID]:28466676
[Au] Autor:Backlund PS; Urbanski HF; Doll MA; Hein DW; Bozinoski M; Mason CE; Coon SL; Klein DC
[Ad] Endereço:Biomedical Mass Spectrometry Facility, Intramural Research Program.
[Ti] Título:Daily Rhythm in Plasma N-acetyltryptamine.
[So] Source:J Biol Rhythms;32(3):195-211, 2017 Jun.
[Is] ISSN:1552-4531
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Normal physiology undergoes 24-h changes in function that include daily rhythms in circulating hormones, most notably melatonin and cortical steroids. This study focused on N-acetyltryptamine, a little-studied melatonin receptor mixed agonist-antagonist and the likely evolutionary precursor of melatonin. The central issue addressed was whether N-acetyltryptamine is physiologically present in the circulation. N-acetyltryptamine was detected by LC-MS/MS in daytime plasma of 3 different mammals in subnanomolar levels (mean ± SEM: rat, 0.29 ± 0.05 nM, n = 5; rhesus macaque, 0.54 ± 0.24 nM, n = 4; human, 0.03 ± 0.01 nM, n = 32). Analysis of 24-h blood collections from rhesus macaques revealed a nocturnal increase in plasma N-acetyltryptamine (p < 0.001), which varied from 2- to 15-fold over daytime levels among the 4 animals studied. Related RNA sequencing studies indicated that the transcript encoding the tryptamine acetylating enzyme arylalkylamine N-acetyltransferase (AANAT) is expressed at similar levels in the rhesus pineal gland and retina, thereby indicating that either tissue could contribute to circulating N-acetyltryptamine. The evidence that N-acetyltryptamine is a physiological component of mammalian blood and exhibits a daily rhythm, together with known effects as a melatonin receptor mixed agonist-antagonist, shifts the status of N-acetyltryptamine from pharmacological tool to candidate for a physiological role. This provides a new opportunity to extend our understanding of 24-h biology.
[Mh] Termos MeSH primário: Ritmo Circadiano
Fotoperíodo
Triptaminas/sangue
[Mh] Termos MeSH secundário: Animais
Arilalquilamina N-Acetiltransferase/genética
Perfilação da Expressão Gênica
Seres Humanos
Macaca mulatta
Masculino
Melatonina/metabolismo
Glândula Pineal/enzimologia
Ratos
Retina/enzimologia
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tryptamines); 1016-47-3 (N-acetyltryptamine); EC 2.3.1.87 (Arylalkylamine N-Acetyltransferase); JL5DK93RCL (Melatonin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1177/0748730417700458



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