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[PMID]:28453635
[Au] Autor:Balasuriya CND; Evensen KAI; Mosti MP; Brubakk AM; Jacobsen GW; Indredavik MS; Schei B; Stunes AK; Syversen U
[Ad] Endereço:Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, 7489 Trondheim, Norway.
[Ti] Título:Peak Bone Mass and Bone Microarchitecture in Adults Born With Low Birth Weight Preterm or at Term: A Cohort Study.
[So] Source:J Clin Endocrinol Metab;102(7):2491-2500, 2017 Jul 01.
[Is] ISSN:1945-7197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Context and Objectives: Peak bone mass (PBM) is regarded as the most important determinant of osteoporosis. Growing evidence suggests a role of intrauterine programming in skeletal development. We examined PBM and trabecular bone score (TBS) in adults born preterm with very low birth weight (VLBW) or small for gestational age (SGA) at term compared with term-born controls. Design, Setting, Participants, and Outcomes: This follow-up cohort study included 186 men and women (25 to 28 years); 52 preterm VLBW (≤1500 g), 59 term-born SGA (<10th percentile), and 75 controls (>10th percentile). Main outcome was bone mineral density (BMD) by dual x-ray absorptiometry. Secondary outcomes were bone mineral content (BMC), TBS, and serum bone markers. Results: VLBW adults had lower BMC and BMD vs controls, also when adjusted for height, weight, and potential confounders, with the following BMD Z-score differences: femoral neck, 0.6 standard deviation (SD) (P = 0.003); total hip, 0.4 SD (P = 0.01); whole body, 0.5 SD (P = 0.007); and lumbar spine, 0.3 SD (P = 0.213). The SGA group displayed lower spine BMC and whole-body BMD Z-scores, but not after adjustment. Adjusted odds ratios for osteopenia/osteoporosis were 2.4 and 2.0 in VLBW and SGA adults, respectively. TBS did not differ between groups, but it was lower in men than in women. Serum Dickkopf-1 was higher in VLBW subjects vs controls; however, it was not significant after adjustment for multiple comparisons. Conclusions: Both low-birth-weight groups displayed lower PBM and higher frequency of osteopenia/osteoporosis, implying increased future fracture risk. The most pronounced bone deficit was seen in VLBW adults.
[Mh] Termos MeSH primário: Densidade Óssea/fisiologia
Desenvolvimento Ósseo/fisiologia
Matriz Óssea/fisiologia
Recém-Nascido Pequeno para a Idade Gestacional/crescimento & desenvolvimento
Recém-Nascido de muito Baixo Peso
Nascimento a Termo
[Mh] Termos MeSH secundário: Absorciometria de Fóton/métodos
Adulto
Fatores Etários
Estudos de Coortes
Intervalos de Confiança
Feminino
Fraturas Espontâneas/epidemiologia
Fraturas Espontâneas/fisiopatologia
Seres Humanos
Recém-Nascido
Modelos Logísticos
Masculino
Razão de Chances
Osteoporose/epidemiologia
Osteoporose/fisiopatologia
Fatores Sexuais
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1210/jc.2016-3827


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[PMID]:28467589
[Au] Autor:Turhan E; Akça MK; Bayar A; Songür M; Keser S; Doral MN
[Ad] Endereço:Department of Orthopedics and Traumatology, Hacettepe University Faculty of Medicine, Ankara-Turkey. dregementurhan@yahoo.com.
[Ti] Título:A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats.
[So] Source:Ulus Travma Acil Cerrahi Derg;23(2):91-99, 2017 Mar.
[Is] ISSN:1306-696X
[Cp] País de publicação:Turkey
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Delayed union of fractured bone is one of the main problems of orthopedics and traumatology practice. It was hypothesized that the beneficial effects of allogeneic platelet-rich plasma (PRP) would be valuable in the treatment of segmental bone defects. This study is a comparison of the effects of demineralized bone matrix (DBM) and PRP in a segmental bone defect model. METHODS: Total of 48 Wistar albino rats were separated into 4 groups. Segmental bone defect was created at right radius diaphysis in all specimens using dorsal approach. Four additional rats were used as PRP source. Intracardiac blood was withdrawn before the operation for preparation of allogeneic PRP. Group 1 (n=12) served as control group and defects were left untreated. Group 2 (n=12), was PRP group, and received grafting with PRP. Group 3 (n=12) was PRP+DBM combination group, and was treated with grafting and mixture of DBM and PRP. In Group 4 (n=12), defect area was grafted with DBM only. At the end of 10th week, rats were sacrificed, forearms were dissected, and defect areas were examined with radiological and histopathological parameters. RESULTS: Radiological evaluation revealed that ossification was best in PRP group, followed by DBM group. According to results of histopathological studies, union quality was better than control group in all treatment groups (Groups 2, 3, and 4), and was best in PRP group (p<0.05). Results were also better in PRP group when examined in terms of cortex development and remodeling (p<0.05). When examined in terms of new osteogenesis, results were comparable in Groups 2, 3, and 4, but all were better than control group. CONCLUSION: It was concluded that PRP and DBM have comparable effect on recovery of defective bones, but there is no synergistic effect when used together. We believe that PRP can be a cost-effective, readily available alternative to DBM with minimal morbidity.
[Mh] Termos MeSH primário: Materiais Biocompatíveis
Matriz Óssea
Fraturas Ósseas/tratamento farmacológico
Osteogênese/efeitos dos fármacos
Plasma Rico em Plaquetas
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/farmacologia
Materiais Biocompatíveis/uso terapêutico
Modelos Animais de Doenças
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.5505/tjtes.2016.68249


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[PMID]:28953728
[Au] Autor:Liang F; Yen SL; Imahiyerobo T; Sanborn L; Yen L; Yen D; Nazarian S; Jedrzejewski B; Urata M; Hammoudeh J
[Ad] Endereço:Los Angeles, Calif.; and Providence, R.I. From the Keck School of Medicine and the School of Dentistry, University of Southern California; the Children's Hospital Los Angeles; and the Warren Alpert Medical School, Brown University.
[Ti] Título:Three-Dimensional Cone Beam Computed Tomography Volumetric Outcomes of rhBMP-2/Demineralized Bone Matrix versus Iliac Crest Bone Graft for Alveolar Cleft Reconstruction.
[So] Source:Plast Reconstr Surg;140(4):767-774, 2017 Oct.
[Is] ISSN:1529-4242
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Recent studies indicate that recombinant human bone morphogenetic protein-2 (rhBMP-2) in a demineralized bone matrix scaffold is a comparable alternative to iliac bone autograft in the setting of secondary alveolar cleft repair. Postreconstruction occlusal radiographs demonstrate improved bone stock when rhBMP-2/demineralized bone matrix (DBM) scaffold is used but lack the capacity to evaluate bone growth in three dimensions. This study uses cone beam computed tomography to provide the first clinical evaluation of volumetric and density comparisons between these two treatment modalities. METHODS: A prospective study was conducted with 31 patients and 36 repairs of the alveolar cleft over a 2-year period. Twenty-one repairs used rhBMP-2/DBM scaffold and 14 repairs used iliac bone grafting. Postoperatively, occlusal radiographs were obtained at 3 months to evaluate bone fill; cone beam computed tomographic images were obtained at 6 to 9 months to compare volumetric and density data. RESULTS: At 3 months, postoperative occlusal radiographs demonstrated that 67 percent of patients receiving rhBMP-2/DBM scaffold had complete bone fill of the alveolus, versus 56 percent of patients in the autologous group. In contrast, cone beam computed tomographic data showed 31.6 percent (95 percent CI, 24.2 to 38.5 percent) fill in the rhBMP-2 group compared with 32.5 percent (95 percent CI, 22.1 to 42.9 percent) in the autologous population. Density analysis demonstrated identical average values between the groups (1.38 g/cc). CONCLUSIONS: These data demonstrate comparable bone regrowth and density values following secondary alveolar cleft repair using rhBMP-2/DBM scaffold versus autologous iliac bone graft. Cone beam computed tomography provides a more nuanced understanding of true bone regeneration within the alveolar cleft that may contribute to the information provided by occlusal radiographs alone. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, II.
[Mh] Termos MeSH primário: Enxerto de Osso Alveolar/métodos
Matriz Óssea/transplante
Proteína Morfogenética Óssea 2/metabolismo
Fissura Palatina/cirurgia
Tomografia Computadorizada de Feixe Cônico/métodos
Ílio/transplante
Imagem Tridimensional
Fator de Crescimento Transformador beta/metabolismo
[Mh] Termos MeSH secundário: Matriz Óssea/metabolismo
Fissura Palatina/diagnóstico
Feminino
Seguimentos
Seres Humanos
Masculino
Estudos Prospectivos
Proteínas Recombinantes/metabolismo
Fatores de Tempo
Transplante Autólogo
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Morphogenetic Protein 2); 0 (Recombinant Proteins); 0 (Transforming Growth Factor beta); 0 (recombinant human bone morphogenetic protein-2)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE
[do] DOI:10.1097/PRS.0000000000003686


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[PMID]:28739717
[Au] Autor:Sobkowicz AD; Sanders AJ; Mason MD; Jiang WG
[Ad] Endereço:Cardiff China Medical Research Collaborative (CCMRC), Cardiff University School of Medicine, Cardiff, U.K.
[Ti] Título:Potential Implication of Paxillin in Cancer Establishment Within the Bone Environment.
[So] Source:Anticancer Res;37(8):4255-4268, 2017 08.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bone metastases are a common feature of advanced prostatic malignancies. They are characterised by a unique prevalence of osteoblastic phenotype and a poor prognosis. Paxillin is a 68-kDa signal transduction adaptor and scaffold protein that contains motifs involved in the mediation of protein-protein interactions. The state of paxillin phosphorylation is central to determining a cell's ability to adhere, detach and migrate and hence has been linked to processes such as wound repair and tumour metastasis. The current study explored the impact of paxillin suppression on prostate and breast cancer cell function and their responsiveness to hepatocyte growth factor (HGF) and bone matrix extract (BME) in order to assess its potential to influence bone colonization and homing. MATERIALS AND METHODS: Hammerhead ribozyme transgenes were used to knockdown the expression of paxillin in breast and prostate cancer cell lines. The impact on the cell growth, migration, adhesion and invasion was assessed using in vitro functional assays. In order to explore potential mechanisms, focal adhesion kinase (FAK) inhibitor was also used. RESULTS: Knockdown of paxillin expression was observed in all tested cell lines following transfection with the ribozyme transgene. The knockdown of paxillin increased proliferation and invasiveness of LNCaP cells, with no effect on their attachment abilities. The opposite, however, is true for PC-3 cells where, following knockdown, cellular attachment was significantly reduced, while no significant changes in growth and invasiveness were detected. In the MDA-MB-231 breast cancer knockdown model, cells had little difference in proliferative rates and generally increased attachment and reduced invasive abilities. Treatments with HGF and BME had differential effects on targeted cells when compared to controls. CONCLUSION: These data suggest that paxillin appears to influence major cell functions in a diverse range of prostate and breast cancer models. The responsiveness of cells to environmental factors such as HGF or BME may be influenced by paxillin status, although this seems to be dependent on cell type.
[Mh] Termos MeSH primário: Neoplasias Ósseas/tratamento farmacológico
Extratos Celulares/administração & dosagem
Fator de Crescimento de Hepatócito/administração & dosagem
Paxilina/genética
[Mh] Termos MeSH secundário: Matriz Óssea/química
Neoplasias Ósseas/genética
Neoplasias Ósseas/patologia
Neoplasias Ósseas/secundário
Neoplasias da Mama/tratamento farmacológico
Neoplasias da Mama/genética
Neoplasias da Mama/patologia
Extratos Celulares/química
Linhagem Celular Tumoral
Movimento Celular/genética
Proliferação Celular/efeitos dos fármacos
Feminino
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Técnicas de Silenciamento de Genes
Seres Humanos
Masculino
Invasividade Neoplásica/genética
Paxilina/antagonistas & inibidores
Neoplasias da Próstata/tratamento farmacológico
Neoplasias da Próstata/genética
Neoplasias da Próstata/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Extracts); 0 (HGF protein, human); 0 (Paxillin); 67256-21-7 (Hepatocyte Growth Factor)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


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[PMID]:28266688
[Au] Autor:Tekari A; May RD; Frauchiger DA; Chan SC; Benneker LM; Gantenbein B
[Ti] Título:The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells.
[So] Source:Eur Cell Mater;33:197-210, 2017 Feb 23.
[Is] ISSN:1473-2262
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:Spinal fusion is hampered by the presence of remaining intervertebral disc (IVD) tissue and leads to spinal non-union. While the exact mechanism remains unknown, we hypothesise that factors preventing disc ossification, such as antagonists of the bone morphogenetic proteins (BMP), could be responsible for this process. The objective of this study was to investigate spinal non-union using an in vitro human model with a focus on the BMP signalling components and to identify factors contributing to the incomplete and delayed ossification. Human bone marrow-derived mesenchymal stromal cells (MSC) were cocultured with IVD cells in the presence of L51P, a BMP2 variant with osteoinductive potential. The ossification of MSC was evaluated by quantitative reverse transcription polymerase chain reaction (qPCR), alkaline phosphatase (ALP) activity and alizarin red staining. Endogenous expression of major BMP antagonists, namely Gremlin (GREM1), Noggin (NOG) and Chordin (CHRD) was detected in IVD-derived cells, with abundance in nucleus pulposus cells. Osteogenesis of MSC was hindered by IVD cells as shown by reduced alizarin red staining, ALP activity and qPCR. L51P, added to the cocultures, restored mineralisation, blocking the activity of the BMP antagonists secreted by IVD cells. It is possible that the BMP antagonists secreted by IVD cells are responsible for spinal non-unions. The inhibition of BMP antagonists with L51P may result in an efficient and more physiological osteoinduction rather than delivery of exogenous osteogenic factors. Therefore, L51P might represent an attractive therapeutic candidate for bone healing.
[Mh] Termos MeSH primário: Proteína Morfogenética Óssea 2/farmacologia
Diferenciação Celular
Disco Intervertebral/citologia
Células Mesenquimais Estromais/citologia
Osteogênese
[Mh] Termos MeSH secundário: Adolescente
Adulto
Fosfatase Alcalina/metabolismo
Biomarcadores/metabolismo
Células da Medula Óssea/citologia
Células da Medula Óssea/efeitos dos fármacos
Células da Medula Óssea/metabolismo
Matriz Óssea/efeitos dos fármacos
Matriz Óssea/metabolismo
Calcificação Fisiológica/efeitos dos fármacos
Diferenciação Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Técnicas de Cocultura
Feminino
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Masculino
Células Mesenquimais Estromais/enzimologia
Meia-Idade
Osteogênese/efeitos dos fármacos
Doadores de Tecidos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BMP2 protein, human); 0 (Biomarkers); 0 (Bone Morphogenetic Protein 2); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE
[do] DOI:10.22203/eCM.v033a15


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[PMID]:28203026
[Au] Autor:Zhan YL; Hu WJ; Xu T; Zhen M; Lu RF
[Ad] Endereço:Department of Periodontology, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
[Ti] Título:[Histomorphometric evaluation of ridge preservation after molar tooth extraction].
[So] Source:Beijing Da Xue Xue Bao Yi Xue Ban;49(1):169-75, 2017 02 18.
[Is] ISSN:1671-167X
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To evaluate bone formation in human extraction sockets with absorbed surrounding walls augmented with Bio-Oss and Bio-Gide after a 6-month healing period by histologic and histomorphometric analyses. METHODS: Six fresh molar tooth extraction sockets in 6 patients who required periodontally compromised moral tooth extraction were included in this study. The six fresh extraction sockets were grafted with Bio-Oss particle covered with Bio-Gide. The 2.8 mm×6.0 mm cylindric bone specimens were taken from the graft sites with aid of stent 6 months after the surgery. Histologic and histomorphometric analyses were performed. RESULTS: The histological results showed Bio-Oss particles were easily distinguished from the newly formed bone, small amounts of new bone were formed among the Bio-Oss particles, large amounts of connective tissue were found. Intimate contact between the newly formed bone and the small part of Bio-Oss particles was present. All the biopsy cylinders measurement demonstrated a high inter-individual variability in the percentage of the bone, connective tissues and Bio-Oss particles. The new bone occupied 11.54% (0-28.40%) of the total area; the connective tissues were 53.42% (34.08%-74.59%) and the Bio-Oss particles were 35.04% (13.92%-50.87%). The percentage of the particles, which were in contact with bone tissues, amounted to 20.13% (0-48.50%). CONCLUSION: Sites grafted with Bio-Oss particles covered with Bio-Gide were comprised of connective tissues and small amounts of newly formed bone surrounding the graft particles.
[Mh] Termos MeSH primário: Matriz Óssea/anatomia & histologia
Matriz Óssea/crescimento & desenvolvimento
Colágeno/farmacologia
Colágeno/uso terapêutico
Tecido Conjuntivo/anatomia & histologia
Tecido Conjuntivo/efeitos dos fármacos
Tecido Conjuntivo/crescimento & desenvolvimento
Minerais/farmacologia
Minerais/uso terapêutico
Alvéolo Dental/anatomia & histologia
Alvéolo Dental/efeitos dos fármacos
Alvéolo Dental/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Matriz Óssea/efeitos dos fármacos
Substitutos Ósseos/uso terapêutico
Seres Humanos
Dente Molar
Osteogênese/efeitos dos fármacos
Osteogênese/fisiologia
Extração Dentária
Alvéolo Dental/lesões
Cicatrização/efeitos dos fármacos
Cicatrização/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bio-Gide); 0 (Bio-Oss); 0 (Bone Substitutes); 0 (Minerals); 9007-34-5 (Collagen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE


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[PMID]:28181209
[Au] Autor:Quade M; Knaack S; Weber D; König U; Paul B; Simon P; Rösen-Wolff A; Schwartz-Albiez R; Gelinsky M; Lode A
[Ti] Título:Heparin modification of a biomimetic bone matrix modulates osteogenic and angiogenic cell response in vitro.
[So] Source:Eur Cell Mater;33:105-120, 2017 Feb 09.
[Is] ISSN:1473-2262
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:In this study, the effect of heparin-modified collagen type I/hydroxyapatite (HA) nanocomposites on key processes of bone regeneration - osteogenesis and angiogenesis - was characterised in vitro. Two approaches were applied for heparin modification: it was either integrated during material synthesis (in situ) or added to the porous scaffolds after their fabrication (post). Cultivation of human bone marrow-derived stromal cells (hBMSC), in heparin-modified versus heparin-free scaffolds, revealed a positive effect of the heparin modification on their proliferation and osteogenic differentiation. The amount of heparin rather than the method used for modification influenced the cell response favouring proliferation at smaller amount (30 mg/g collagen) and differentiation at larger amount (150 mg/g collagen). A co-culture of human umbilical vein endothelial cells (HUVEC) and osteogenically induced hBMSC was applied for in vitro angiogenesis studies. Pre-vascular networks have formed in the porous structure of scaffolds which were not modified with heparin or modified with a low amount of heparin (30 mg/g collagen). The modification with higher heparin quantities seemed to inhibit tubule formation. Pre-loading of the scaffolds with VEGF influenced formation and stability of the pre-vascular structures depending on the presence of heparin: In heparin-free scaffolds, induction of tubule formation and sprouting was more pronounced whereas heparin-modified scaffolds seemed to promote stabilisation of the pre-vascular structures. In conclusion, the modification of mineralised collagen with heparin by using both approaches was found to modulate cellular processes essential for bone regeneration; the amount of heparin has been identified to be crucial to direct cell responses.
[Mh] Termos MeSH primário: Materiais Biomiméticos/farmacologia
Matriz Óssea/metabolismo
Heparina/farmacologia
Células Endoteliais da Veia Umbilical Humana/citologia
Neovascularização Fisiológica/efeitos dos fármacos
Osteogênese/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adulto
Fosfatase Alcalina/metabolismo
Animais
Matriz Óssea/efeitos dos fármacos
Bovinos
Diferenciação Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Técnicas de Cocultura
Colágeno/farmacologia
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
Masculino
Teste de Materiais
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/efeitos dos fármacos
Microscopia de Fluorescência
Tecidos Suporte/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9005-49-6 (Heparin); 9007-34-5 (Collagen); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE
[do] DOI:10.22203/eCM.v033a08


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[PMID]:28162888
[Au] Autor:Thangarajah T; Henshaw F; Sanghani-Kerai A; Lambert SM; Blunn GW; Pendegrass CJ
[Ad] Endereço:The John Scales Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, Division of Surgery and Interventional Science, University College London, The Royal National Orthopaedic Hospital Trust, Stanmore, Middlesex, UK. Electronic address: tanujan1@hotmail.com.
[Ti] Título:The effectiveness of demineralized cortical bone matrix in a chronic rotator cuff tear model.
[So] Source:J Shoulder Elbow Surg;26(4):619-626, 2017 Apr.
[Is] ISSN:1532-6500
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The purpose of this study was to assess the effect of demineralized bone matrix (DBM) on rotator cuff tendon-bone healing. The hypothesis was that compared with a commercially available dermal matrix scaffold, DBM would result in a higher bone mineral density and regenerate a morphologically superior enthesis in a rat model of chronic rotator cuff degeneration. METHODS: Eighteen female Wistar rats underwent unilateral detachment of the supraspinatus tendon. Three weeks later, tendon repair was carried out in animals randomized into 3 groups: group 1 animals were repaired with DBM (n = 6); group 2 received augmentation with the dermal scaffold (n = 6); and group 3 (controls) underwent nonaugmented tendon-bone repair (n = 6). Specimens were retrieved at 6 weeks postoperatively for histologic analysis and evaluation of bone mineral density. RESULTS: No failures of tendon-bone healing were noted throughout the study. All groups demonstrated closure of the tendon-bone gap with a fibrocartilaginous interface. Dermal collagen specimens exhibited a disorganized structure with significantly more abnormal collagen fiber arrangement and cellularity than in the DBM-based repairs. Nonaugmented repairs exhibited a significantly higher bone mineral density than in DBM and the dermal collagen specimens and were not significantly different from control limbs that were not operated on. CONCLUSION: The application of DBM to a rat model of chronic rotator cuff degeneration did not improve the composition of the healing enthesis compared with nonaugmented controls and a commercially available scaffold. However, perhaps the most important finding of this study was that the control group demonstrated a similar outcome to augmented repairs.
[Mh] Termos MeSH primário: Matriz Óssea
Lesões do Manguito Rotador/terapia
Tecidos Suporte
Cicatrização
[Mh] Termos MeSH secundário: Derme Acelular
Animais
Materiais Biocompatíveis
Densidade Óssea
Doença Crônica
Osso Cortical
Feminino
Ratos
Ratos Wistar
Lesões do Manguito Rotador/cirurgia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170207
[St] Status:MEDLINE


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[PMID]:28098926
[Au] Autor:Bernhardt A; Koperski K; Schumacher M; Gelinsky M
[Ad] Endereço:Fetscherstr. 42, 01309 Dresden, Germany.anne.bernhardt@tu-dresden.de.
[Ti] Título:Relevance of osteoclast-specific enzyme activities in cell-based in vitro resorption assays.
[So] Source:Eur Cell Mater;33:28-42, 2017 Jan 18.
[Is] ISSN:1473-2262
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:Cell-based in vitro resorption assays are an important tool to simulate the in vivo biodegradation of resorbable bone graft materials and to predict their clinical performance. The present study analyses the activity of osteoclast-specific enzymes as potential surrogate measures for classical pit assay, which is not applicable on irregular structured materials. Osteoclasts derived from human peripheral blood mononuclear cells were cultivated on different surfaces: calcium phosphate bone cements (CPC), dentin discs, osteoblast-derived extracellular matrix (ECM) and tissue culture polystyrene as control. Pit formation on the resorbable materials was investigated and correlated with the activity of tartrate resistant acid phosphatase (TRAP), carbonic anhydrase II (CAII) and cathepsin K (CTSK). Furthermore, the relation between intra- and extracellular enzyme activities was examined for TRAP and CTSK during resorption of the different materials. Resorbed area of CPC correlated with intracellular TRAP activity and intracellular CAII activity. Highest resorption was detected at around pH 7.2. Resorbed area on dentin correlated with the extracellular CTSK activity and extracellular TRAP activity and was maximal at around pH 6.8. Osteoclasts cultivated on cell-derived mineralised ECM showed a good correlation between both extracellular TRAP and CTSK activity and the release of calcium ions. Based on these data a different regulation of TRAP and CTSK secretion is hypothesised for the resorption of inorganic calcium phosphate compared to the resorption of collagenous mineralised matrix.
[Mh] Termos MeSH primário: Bioensaio/métodos
Reabsorção Óssea/enzimologia
Osteoclastos/enzimologia
[Mh] Termos MeSH secundário: Cimentos para Ossos/farmacologia
Matriz Óssea/efeitos dos fármacos
Matriz Óssea/metabolismo
Reabsorção Óssea/patologia
Fosfatos de Cálcio/farmacologia
Diferenciação Celular/efeitos dos fármacos
Dentina/metabolismo
Matriz Extracelular/efeitos dos fármacos
Matriz Extracelular/metabolismo
Seres Humanos
Osteoblastos/efeitos dos fármacos
Osteoblastos/enzimologia
Osteoblastos/patologia
Osteoclastos/efeitos dos fármacos
Osteoclastos/patologia
Osteoclastos/ultraestrutura
Poliestirenos/farmacologia
Coloração e Rotulagem
Fosfatase Ácida Resistente a Tartarato/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Cements); 0 (Calcium Phosphates); 0 (Polystyrenes); 97Z1WI3NDX (calcium phosphate); EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.22203/eCM.v033a03


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[PMID]:27943619
[Au] Autor:Dozza B; Lesci IG; Duchi S; Della Bella E; Martini L; Salamanna F; Falconi M; Cinotti S; Fini M; Lucarelli E; Donati D
[Ad] Endereço:Osteoarticolar Regeneration Laboratory, 3rd Orthopaedic and Traumatologic Clinic Prevalently Oncologic, Rizzoli Orthopaedic Institute, via di Barbiano 1/10, Bologna, 40136, Italy.
[Ti] Título:When size matters: differences in demineralized bone matrix particles affect collagen structure, mesenchymal stem cell behavior, and osteogenic potential.
[So] Source:J Biomed Mater Res A;105(4):1019-1033, 2017 Apr.
[Is] ISSN:1552-4965
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Demineralized bone matrix (DBM) is a natural, collagen-based, osteoinductive biomaterial. Nevertheless, there are conflicting reports on the efficacy of this product. The purpose of this study was to evaluate whether DBM collagen structure is affected by particle size and can influence DBM cytocompatibility and osteoinductivity. Sheep cortical bone was ground and particles were divided in three fractions with different sizes, defined as large (L, 1-2 mm), medium (M, 0.5-1 mm), and small (S, <0.5 mm). After demineralization, the chemical-physical analysis clearly showed a particle size-dependent alteration in collagen structure, with DBM-M being altered but not as much as DBM-S. DBM-M displayed a preferable trend in almost all biological characteristics tested, although all DBM particles revealed an optimal cytocompatibility. Subcutaneous implantation of DBM particles into immunocompromised mice resulted in bone induction only for DBM-M. When sheep MSC were seeded onto particles before implantation, all DBM particles were able to induce new bone formation with the best incidence for DBM-M and DBM-S. In conclusion, the collagen alteration in DBM-M is likely the best condition to promote bone induction in vivo. Furthermore, the choice of 0.5-1 mm particles may enable to obtain more efficient and consistent results among different research groups in bone tissue-engineering applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1019-1033, 2017.
[Mh] Termos MeSH primário: Matriz Óssea/citologia
Colágeno/química
Células Mesenquimais Estromais/metabolismo
Osteogênese
[Mh] Termos MeSH secundário: Animais
Matriz Óssea/transplante
Camundongos
Camundongos SCID
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-34-5 (Collagen)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1002/jbm.a.35975



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