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Pesquisa : A10.165.265.200 [Categoria DeCS]
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  1 / 2098 MEDLINE  
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[PMID]:28464615
[Au] Autor:Nygren H; Bigdeli N; Ilver L; Malmberg P
[Ad] Endereço:Department of Medical Chemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, POB 440, 40530 Gothenburg, Sweden.
[Ti] Título:Mg-corrosion, hydroxyapatite, and bone healing.
[So] Source:Biointerphases;12(2):02C407, 2017 05 02.
[Is] ISSN:1559-4106
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The different capacities of magnesium in the metallic form (Mg-metal) and magnesium oxide (MgO) to stimulate bone healing are possible clues in the search for products that may promote bone healing. Since both Mg-metal and MgO can be assumed to release comparable amounts of Mg ions during their reactions in the tissue where they have been implanted, it is of some importance to follow this process and analyze the resulting mineral formation in the tissue at the implantation site. Implants of MgO were inserted into rat tibia, and the bone healing was compared with sham-operated controls. Samples were taken after 1 week of healing and analyzed by histology, environmental scanning electron microscopy equipped with an energy dispersive x-ray spectroscopy analyzer, and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Callus bone was seen in sham-operated controls after 1 week of healing. Implantation of MgO impaired the callus bone formation by replacing bone with apparently mineralized areas, lacking osteocytes and were denoted, amorphous bodies. Elemental analysis showed increased levels of Ca (7.1%), P (3.7%), and Mg (0.2%) in the bone marrow of MgO-treated animals versus sham-operated controls Ca (2.4%), P (2.3%), and Mg (0.1%). The Ca content of the cortical bone was also significantly increased (Ca, 29% increase) in MgO-treated animals compared to sham-operated controls. The Ca content of the cortical bone of sham-operated animals was also significantly (p < 0.05) higher than the corresponding value of untreated animals, which means that the surgical trauma induces an altered composition of the bone mineral. The Ca/P ratio was 1.26-1.68, which is compatible with that of mineralized bone with different contents of organic materials. Analysis of bone sections using ToF-SIMS showed the presence of hydroxyapatite (HA) and MgCO in the bone marrow and in cortical bone. Analysis using x-ray photoelectron spectroscopy of Mg, MgO, and MgCO after incubation with cell culture medium (DMEM), in vitro, showed binding of CaPO at the Mg and MgO samples. The Ca/P ratio was 0.8, indicating a higher P content than that expected for HA. Exposure of human embryonic stem cells to Mg species preincubated in DMEM resulted in HA production by the cells. Thus, two sources of CaPO in the bone marrow of MgO-treated bone were defined, catalytic formation on Mg-species and synthesis from activated stem-cells. The presented data suggest that bone healing near Mg implants is congruent with the fracture healing of bone, boosted by high HA levels in the bone marrow. In this context, the different capacities of Mg-metal and MgO to catalyse the formation of HA can be important clues to their different bone promoting effects.
[Mh] Termos MeSH primário: Calo Ósseo/metabolismo
Osso Cortical/lesões
Osso Cortical/metabolismo
Durapatita/farmacologia
Consolidação da Fratura/efeitos dos fármacos
Óxido de Magnésio/farmacologia
[Mh] Termos MeSH secundário: Animais
Calo Ósseo/patologia
Linhagem Celular
Corrosão
Osso Cortical/patologia
Células-Tronco Embrionárias Humanas
Seres Humanos
Magnésio/farmacologia
Masculino
Teste de Materiais
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
3A3U0GI71G (Magnesium Oxide); 91D9GV0Z28 (Durapatite); I38ZP9992A (Magnesium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1116/1.4982601


  2 / 2098 MEDLINE  
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Volpon, José Batista
Texto completo SciELO Brasil
[PMID]:29236797
[Au] Autor:Guimarães APFGM; Butezloff MM; Zamarioli A; Issa JPM; Volpon JB
[Ad] Endereço:Fellow Master degree, Postgraduate Program in Health Sciences Applied to the Locomotor System, School of Medicine, Universidade de São Paulo (USP), Ribeirao Preto-SP, Brazil. Design of the study, technical procedures, acquisition and interpretation of data, manuscript preparation.
[Ti] Título:Nandrolone decanoate appears to increase bone callus formation in young adult rats after a complete femoral fracture.
[So] Source:Acta Cir Bras;32(11):924-934, 2017 Nov.
[Is] ISSN:1678-2674
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To evaluate the influence of nandrolone decanoate on fracture healing and bone quality in normal rats. METHODS: Male rats were assigned to four groups (n=28/group): Control group consisting of animals without any intervention, Nandrolone decanoate (DN) group consisting of animals that received intramuscular injection of nandrolone decanoate, Fracture group consisting of animals with a fracture at the mid-diaphysis of the femur, and Fracture and nandrolone decanoate group consisting of animals with a femur fracture and treatment with nandrolone decanoate. Fractures were created at the mid-diaphysis of the right femur by a blunt trauma and internally fixed using an intramedullary steel wire. The DN was injected intramuscularly twice per week (10 mg/kg of body mass). The femurs were measured and evaluated by densitometry and mechanical resistance after animal euthanasia. The newly formed bone and collagen type I levels were quantified in the callus. RESULTS: The treated animals had longer femurs after 28 days. The quality of the intact bone was not significantly different between groups. The bone callus did show a larger mass in the treated rats. CONCLUSION: The administration of nandrolone decanoate did not affect the quality of the intact bone, but might have enhanced the bone callus formation.
[Mh] Termos MeSH primário: Anabolizantes/farmacologia
Calo Ósseo/fisiologia
Fraturas do Fêmur/tratamento farmacológico
Consolidação da Fratura/efeitos dos fármacos
Nandrolona/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Densidade Óssea/fisiologia
Consolidação da Fratura/fisiologia
Masculino
Nandrolona/farmacologia
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anabolic Agents); 6PG9VR430D (Nandrolone); H45187T098 (nandrolone decanoate)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE


  3 / 2098 MEDLINE  
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[PMID]:29020057
[Au] Autor:Nishitani K; Mietus Z; Beck CA; Ito H; Matsuda S; Awad HA; Ehrhart N; Schwarz EM
[Ad] Endereço:Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY, United States of America.
[Ti] Título:High dose teriparatide (rPTH1-34) therapy increases callus volume and enhances radiographic healing at 8-weeks in a massive canine femoral allograft model.
[So] Source:PLoS One;12(10):e0185446, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Small animal studies have demonstrated significant high-dose recombinant parathyroid hormone1-34 (rPTH1-34) effects on intercalary allograft healing. Towards a human adjuvant therapy to decrease non-unions, we evaluated rPTH1-34 safety and efficacy in a clinically relevant canine femoral allograft model. Adult female mongrel hounds (n = 20) received a 5cm mid-diaphyseal osteotomy reconstructed with a plated allograft, and were randomized to: 1) Placebo (n = 5; daily saline), 2) Continuous rPTH1-34 (n = 7; 5 µg/kg/day s.c. from day 1-55 post-op), or 3) Delayed rPTH1-34 (n = 8; 5 µg/kg/day s.c. from day 14-28 post-op). Safety was assessed by physical behavior and blood calcium monitoring. Cone beam CT (CB-CT) was performed on days 14, 28 and 56 post-op to assess 2D cortical healing, 3D bone volume, and Union Ratio. Biomechanical testing and dynamic histomorphometry were also performed. The high drug dose was poorly tolerated, as most dogs receiving rPTH1-34 had to be given intravenous saline, and one dog died from hypercalcemia. Continuous rPTH1-34 significantly increased 2D healing and callus volumes at 4-weeks versus Placebo, and sustained the significant increase in cortical union at 8-week (p<0.05). These rPTH1-34 effects were confirmed by histomorphometry, revealing significant increases in mineral apposition rates (MAR) on host bone and graft-host junctions (p<0.05). Delayed rPTH1-34 significantly increased callus volume and MAR at 8 weeks (p<0.05). Although no biomechanical differences were observed, as expected for early healing, the results demonstrated that 2D RUST scoring significantly correlated with torsional biomechanics (p<0.01). In conclusion, 8-weeks of intermittent high-dose rPTH1-34 treatment significantly increases callus formation and accelerates bony union of intercalary massive allografts in a clinically relevant canine model, but with serious side-effects from hypercalcemia.
[Mh] Termos MeSH primário: Calo Ósseo/diagnóstico por imagem
Calo Ósseo/patologia
Fêmur/transplante
Teriparatida/administração & dosagem
Teriparatida/uso terapêutico
Cicatrização
[Mh] Termos MeSH secundário: Aloenxertos/efeitos dos fármacos
Animais
Fenômenos Biomecânicos/efeitos dos fármacos
Calo Ósseo/efeitos dos fármacos
Tomografia Computadorizada de Feixe Cônico
Modelos Animais de Doenças
Cães
Relação Dose-Resposta a Droga
Fêmur/fisiopatologia
Fêmur/cirurgia
Fluorescência
Minerais/metabolismo
Cuidados Pós-Operatórios
Teriparatida/farmacologia
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Minerals); 10T9CSU89I (Teriparatide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185446


  4 / 2098 MEDLINE  
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[PMID]:28178186
[Au] Autor:Wang Y; Fang X; Wang C; Ding C; Lin H; Liu A; Wang L; Cao Y
[Ad] Endereço:Department of Orthopaedic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China. milkyway199@hotmail.com.
[Ti] Título:Exogenous PTHrP Repairs the Damaged Fracture Healing of PTHrP+/- Mice and Accelerates Fracture Healing of Wild Mice.
[So] Source:Int J Mol Sci;18(2), 2017 Feb 06.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Bone fracture healing is a complicated physiological regenerative process initiated in response to injury and is similar to bone development. To demonstrate whether an exogenous supply of parathyroid hormone-related protein (PTHrP) helps in bone fracture healing, closed mid-diaphyseal femur fractures were created and stabilized with intramedullary pins in eight-week-old wild-type (WT) PTHrP+/+ and PTHrP+/- mice. After administering PTHrP for two weeks, callus tissue properties were analyzed at one, two, and four weeks post-fracture (PF) by various methods. Bone formation-related genes and protein expression levels were evaluated by real-time reverse transcriptase-polymerase chain reaction and Western blots. At two weeks PF, mineral density of callus, bony callus areas, mRNA levels of alkaline phosphatase (ALP), type I collagen, Runt-related transcription factor 2 (Runx-2), and protein levels of Runx-2 and insulin-like growth factor-1 decreased in PTHrP+/- mice compared with WT mice. At four weeks PF, total collagen-positive bony callus areas, osteoblast number, ALP-positive areas, and type I collagen-positive areas all decreased in PTHrP+/- mice. At both two and four weeks PF, tartrate-resistant acid phosphatase-positive osteoclast number and surface decreased a little in PTHrP+/- mice. The study indicates that exogenous PTHrP provided by subcutaneous injection could redress impaired bone fracture healing, leading to mutation of activated PTHrP by influencing callus areas, endochondral bone formation, osteoblastic bone formation, and bone turnover.
[Mh] Termos MeSH primário: Consolidação da Fratura/efeitos dos fármacos
Consolidação da Fratura/genética
Proteína Relacionada ao Hormônio Paratireóideo/genética
Proteína Relacionada ao Hormônio Paratireóideo/farmacologia
[Mh] Termos MeSH secundário: Animais
Densidade Óssea
Desenvolvimento Ósseo/genética
Reabsorção Óssea/genética
Reabsorção Óssea/metabolismo
Calo Ósseo/efeitos dos fármacos
Calo Ósseo/metabolismo
Cartilagem/citologia
Cartilagem/metabolismo
Diferenciação Celular
Fraturas Ósseas
Expressão Gênica
Camundongos
Camundongos Knockout
Modelos Animais
Osteoblastos/efeitos dos fármacos
Osteoblastos/metabolismo
Osteoclastos/efeitos dos fármacos
Osteoclastos/metabolismo
Osteogênese/efeitos dos fármacos
Osteogênese/genética
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Parathyroid Hormone-Related Protein)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE


  5 / 2098 MEDLINE  
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[PMID]:28096214
[Au] Autor:Hu DP; Ferro F; Yang F; Taylor AJ; Chang W; Miclau T; Marcucio RS; Bahney CS
[Ad] Endereço:University of California, San Francisco (UCSF) & San Francisco General Hospital (SFGH), Department of Orthopaedic Surgery, Orthopaedic Trauma Institute, 2550 23rd Street, Building 9, 3rd Floor, San Francisco, CA 94110, USA.
[Ti] Título:Cartilage to bone transformation during fracture healing is coordinated by the invading vasculature and induction of the core pluripotency genes.
[So] Source:Development;144(2):221-234, 2017 01 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fractures heal predominantly through the process of endochondral ossification. The classic model of endochondral ossification holds that chondrocytes mature to hypertrophy, undergo apoptosis and new bone forms by invading osteoprogenitors. However, recent data demonstrate that chondrocytes transdifferentiate to osteoblasts in the growth plate and during regeneration, yet the mechanism(s) regulating this process remain unknown. Here, we show a spatially-dependent phenotypic overlap between hypertrophic chondrocytes and osteoblasts at the chondro-osseous border in the fracture callus, in a region we define as the transition zone (TZ). Hypertrophic chondrocytes in the TZ activate expression of the pluripotency factors [Sox2, Oct4 (Pou5f1), Nanog], and conditional knock-out of Sox2 during fracture healing results in reduction of the fracture callus and a delay in conversion of cartilage to bone. The signal(s) triggering expression of the pluripotency genes are unknown, but we demonstrate that endothelial cell conditioned medium upregulates these genes in ex vivo fracture cultures, supporting histological evidence that transdifferentiation occurs adjacent to the vasculature. Elucidating the cellular and molecular mechanisms underlying fracture repair is important for understanding why some fractures fail to heal and for developing novel therapeutic interventions.
[Mh] Termos MeSH primário: Transdiferenciação Celular/genética
Condrócitos/fisiologia
Neovascularização Fisiológica/fisiologia
Osteoblastos/fisiologia
Osteogênese/fisiologia
Células-Tronco Pluripotentes/fisiologia
[Mh] Termos MeSH secundário: Animais
Osso e Ossos/citologia
Osso e Ossos/fisiologia
Calo Ósseo/crescimento & desenvolvimento
Calo Ósseo/metabolismo
Cartilagem/citologia
Cartilagem/fisiologia
Células Cultivadas
Condrócitos/citologia
Condrogênese/fisiologia
Consolidação da Fratura/genética
Consolidação da Fratura/fisiologia
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Neovascularização Fisiológica/genética
Osteoblastos/citologia
Regulação para Cima/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1242/dev.130807


  6 / 2098 MEDLINE  
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[PMID]:28085103
[Au] Autor:Moreno-Anzúrez NE; Marquina S; Alvarez L; Zamilpa A; Castillo-España P; Perea-Arango I; Torres PN; Herrera-Ruiz M; Díaz García ER; García JT; Arellano-García J
[Ad] Endereço:Centro Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col, Chamilpa C.P. 62209, Cuernavaca, Morelos, Mexico. norma.moreno@uaem.mx.
[Ti] Título:A Cytotoxic and Anti-inflammatory Campesterol Derivative from Genetically Transformed Hairy Roots of Lopezia racemosa Cav. (Onagraceae).
[So] Source:Molecules;22(1), 2017 Jan 12.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The genetically transformed hairy root line LRT 7.31 obtained by infecting leaf explants of Cav with the strain ATCC15834/pTDT, was evaluated to identify the anti-inflammatory and cytotoxic compounds reported previously for the wild plant. After several subcultures of the LRT 7.31 line, the bio-guided fractionation of the dichloromethane-methanol (1:1) extract obtained from dry biomass afforded a fraction that showed important in vivo anti-inflammatory, and in vitro cytotoxic activities. Chemical separation of the active fraction allowed us to identify the triterpenes ursolic ( ) and oleanolic ( ) acids, and (23 )-2α,3ß,23,28-tetrahydroxy-14,15-dehydrocampesterol ( ) as the anti-inflammatory principles of the active fraction. A new molecule was characterized by spectroscopic analysis of its tetraacetate derivative . This compound was not described in previous reports of callus cultures, in vitro germinated seedlings and wild plant extracts of whole plants. The anti-inflammatory and cytotoxic activities displayed by the fraction are associated to the presence of compounds - . The present study reports the obtaining of the transformed hairy roots, the bioguided isolation of the new molecule , and its structure characterization.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Proliferação Celular/efeitos dos fármacos
Colesterol/análogos & derivados
Germinação/efeitos dos fármacos
Onagraceae/química
Fitosteróis/farmacologia
[Mh] Termos MeSH secundário: Agrobacterium/química
Agrobacterium/genética
Anti-Inflamatórios/química
Calo Ósseo/efeitos dos fármacos
Calo Ósseo/crescimento & desenvolvimento
Colesterol/química
Colesterol/farmacologia
Ácido Oleanólico/química
Ácido Oleanólico/farmacologia
Fitosteróis/química
Raízes de Plantas/química
Plantas Geneticamente Modificadas/efeitos dos fármacos
Plântulas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Phytosterols); 5L5O665639 (campesterol); 6SMK8R7TGJ (Oleanolic Acid); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170114
[St] Status:MEDLINE


  7 / 2098 MEDLINE  
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[PMID]:28054333
[Au] Autor:Orth M; Kruse NJ; Braun BJ; Scheuer C; Holstein JH; Khalil A; Yu X; Murphy WL; Pohlemann T; Laschke MW; Menger MD
[Ad] Endereço:Department of Trauma, Hand and Reconstructive Surgery, Saarland University, Kirrberger Strasse 1, D-66421 Homburg/Saar, Germany.marcel.orth@uks.eu.
[Ti] Título:BMP-2-coated mineral coated microparticles improve bone repair in atrophic non-unions.
[So] Source:Eur Cell Mater;33:1-12, 2017 Jan 02.
[Is] ISSN:1473-2262
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:Atrophic non-unions are a major clinical problem. Mineral coated microparticles (MCM) are electrolyte-coated hydroxyapatite particles that have been shown in vitro to bind growth factors electrostatically and enable a tuneable sustained release. Herein, we studied whether MCM can be used in vivo to apply Bone Morphogenetic Protein-2 (BMP-2) to improve bone repair of atrophic non-unions. For this purpose, atrophic non-unions were induced in femurs of CD-1 mice (n = 48). Animals either received BMP-2-coated MCM (MCM + BMP; n = 16), uncoated MCM (MCM; n = 16) or no MCM (NONE; n = 16). Bone healing was evaluated 2 and 10 weeks postoperatively by micro-computed tomographic (µCT), biomechanical, histomorphometric and immunohistochemical analyses. µCT revealed more bone volume with more highly mineralised bone in MCM + BMP femurs. Femurs of MCM + BMP animals showed a significantly higher bending stiffness compared to other groups. Histomorphometry further demonstrated that the callus of MCM + BMP femurs was larger and contained more bone and less fibrous tissue. After 10 weeks, 7 of 8 MCM + BMP femurs presented with complete osseous bridging, whereas NONE femurs exhibited a non-union rate of 100 %. Of interest, immunohistochemistry could not detect macrophages within the callus, indicating a good biocompatibility of MCM. In conclusion, the local application of BMP-2-coated MCM improved bone healing in a challenging murine non-union model and, thus, should be of clinical interest in the treatment of non-unions.
[Mh] Termos MeSH primário: Proteína Morfogenética Óssea 2/farmacologia
Materiais Revestidos Biocompatíveis/farmacologia
Consolidação da Fratura/efeitos dos fármacos
Fraturas não Consolidadas/patologia
Microesferas
Minerais/farmacologia
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos/efeitos dos fármacos
Líquidos Corporais/química
Proteína Morfogenética Óssea 2/administração & dosagem
Osso e Ossos/efeitos dos fármacos
Osso e Ossos/patologia
Calo Ósseo/efeitos dos fármacos
Calo Ósseo/patologia
Materiais Revestidos Biocompatíveis/administração & dosagem
Preparações de Ação Retardada
Fêmur/diagnóstico por imagem
Fêmur/efeitos dos fármacos
Fêmur/patologia
Fêmur/fisiopatologia
Fraturas não Consolidadas/fisiopatologia
Imuno-Histoquímica
Cinética
Camundongos
Microscopia Eletrônica de Varredura
Osteotomia
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Morphogenetic Protein 2); 0 (Coated Materials, Biocompatible); 0 (Delayed-Action Preparations); 0 (Minerals)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.22203/eCM.v033a01


  8 / 2098 MEDLINE  
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Volpon, José B
Texto completo
[PMID]:27905059
[Au] Autor:Santiago HA; Zamarioli A; Sousa Neto MD; Volpon JB
[Ad] Endereço:Laboratory of Bioengineering, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
[Ti] Título:Exposure to Secondhand Smoke Impairs Fracture Healing in Rats.
[So] Source:Clin Orthop Relat Res;475(3):894-902, 2017 Mar.
[Is] ISSN:1528-1132
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Nonsmokers may be affected by environmental tobacco smoke (secondhand smoke), but the effects of such exposure on fracture healing have not been well studied. QUESTIONS/PURPOSES: To explore the possible effects of passive inhalation of tobacco smoke on the healing of a diaphyseal fracture in femurs of rats. We hypothesized that secondhand exposure to tobacco smoke adversely affects fracture healing. METHODS: A mid-diaphyseal fracture was created in the femur of 41 female Wistar rats and fixed with an intramedullary metallic pin; 14 rats were excluded (nine for inadequate fractures and five for K wire extrusion). Tobacco exposure was provided by a smoking machine on a daily basis of four cigarettes a day. Each cigarette yielded 10 mg tar and 0.8 mg nicotine, and was puffed by alternating injections of fresh air for 30 seconds and smoke air for 15 seconds. The smoke exposure was previously adjusted to provide serum levels of cotinine similar to human secondhand tobacco exposure. Cotinine is a predominant catabolite of nicotine that is used as a biological biomarker for exposure to tobacco smoke. In one group (n = 11), the animals were intermittently exposed to tobacco smoke before sustaining the fracture but not afterward. In another group (n = 7), the exposure occurred before and after the fracture. The control group (n = 9) was sham-exposed before and after the fracture. We evaluated the specimens 28 days after bone fracture. The callus quality was measured by dual-energy x-ray absorptiometry (bone mineral density [BMD], bone mineral content [BMC], and callus area), µCT (callus volume and woven bone fraction), and mechanical bending (maximum force and stiffness). RESULTS: Tobacco exposure resulted in delayed bone callus formation, which is represented by decreased BMD (Control: 0.302 ± 0.008 g/cm vs Preexposed: 0.199 ± 0.008 g/cm and Pre- and Postexposed: 0.146 ± 0.009 g/cm ; mean difference = 0.103 g/cm , 95% CI, 0.094-0.112 g/cm and mean difference = 0.156 g/cm , 95% CI, 0.147-0.167 g/cm ; p < 0.01), BMC (Control: 0.133 ± 0.005 g vs Preexposed: 0.085 ± 0.0034 g and Pre- and Postexposed: 0.048 ± 0.003 g; mean difference = 0.048 g, 95% CI, 0.045-0.052 g and mean difference = 0.085 g, 95% CI, 0.088-0.090 g; p < 0.01), callus volume (Control: 7.656 ± 1.963 mm vs Preexposed: 17.952 ± 1.600 mm and Pre- and Postexposed: 40.410 ± 3.340 mm ; mean difference = -10.30 mm , 95% CI, -14.12 to 6.471 mm and mean difference, -32.75 mm , 95% CI, -36.58 to 28.93 mm ; p < 0.01), woven bone fraction (Control: 42.076 ± 3.877% vs Preexposed: 16.655 ± 3.021% and Pre- and Postexposed: 8.015 ± 1.565%, mean difference = 0.103%, 95% CI, 0.094-0.112% and mean difference = 0.156%, 95% CI, 0.147-0.166%; p < 0.01), maximum force (Control: 427.122 ± 63.952 N.mm vs Preexposed: 149.230 ± 67.189 N.mm and Pre- and Postexposed: 123.130 ± 38.206 N.mm, mean difference = 277.9 N.mm, 95% CI, 201.1-354.7 N.mm and mean difference = 304 N.mm, 95% CI, 213.2-394.8 N.mm; p < 0.01) and stiffness (Control: 491.397 ± 96.444 N.mm/mm vs Preexposed: 73.157 ± 36.511 N.mm/mm and Pre- and Postexposed: 154.049 ± 134.939 N.mm/mm, mean difference = 418.2 N.mm/mm, 95% CI, 306.3-530.1 N.mm/mm and mean difference = 337.3 N.mm/mm, 95% CI, 188.8-485.9 N.mm/mm; p < 0. 01). CONCLUSIONS: Rats exposed to tobacco smoke showed delayed fracture healing and callus that was characterized by decreased maturity, density, and mechanical resistance, which was confirmed by all assessment methods of this study. Such effects were more evident when animals were exposed to tobacco smoke before and after the fracture. Future studies should be done in human passive smokers to confirm or refute our findings on fracture callus formation. CLINICAL RELEVANCE: The potential hazardous effects of secondhand smoke on fracture healing in rodents should stimulate future clinical studies in human passive smokers.
[Mh] Termos MeSH primário: Fraturas do Fêmur/cirurgia
Fêmur/cirurgia
Consolidação da Fratura
Poluição por Fumaça de Tabaco/efeitos adversos
[Mh] Termos MeSH secundário: Absorciometria de Fóton
Animais
Fenômenos Biomecânicos
Densidade Óssea
Pinos Ortopédicos
Calo Ósseo/diagnóstico por imagem
Calo Ósseo/fisiopatologia
Modelos Animais de Doenças
Feminino
Fraturas do Fêmur/diagnóstico por imagem
Fraturas do Fêmur/fisiopatologia
Fêmur/diagnóstico por imagem
Fêmur/fisiopatologia
Fixação Intramedular de Fraturas/instrumentação
Exposição por Inalação/efeitos adversos
Ratos Wistar
Fatores de Tempo
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tobacco Smoke Pollution)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE
[do] DOI:10.1007/s11999-016-5184-6


  9 / 2098 MEDLINE  
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[PMID]:27864378
[Au] Autor:McKenzie JA; Buettmann E; Abraham AC; Gardner MJ; Silva MJ; Killian ML
[Ad] Endereço:Department of Orthopedic Surgery, Washington University School of Medicine, St. Louis, Missouri, USA.
[Ti] Título:Loss of scleraxis in mice leads to geometric and structural changes in cortical bone, as well as asymmetry in fracture healing.
[So] Source:FASEB J;31(3):882-892, 2017 Mar.
[Is] ISSN:1530-6860
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Scleraxis (Scx) is a known regulator of tendon development, and recent work has identified the role of Scx in bone modeling. However, the role of Scx in fracture healing has not yet been explored. This study was conducted to identify the role of Scx in cortical bone development and fracture healing. Scx green fluorescent protein-labeled (ScxGFP) reporter and Scx-knockout (Scx-mutant) mice were used to assess bone morphometry and the effects of fracture healing on Scx localization and gene expression, as well as callus healing response. Botulinum toxin (BTX) was used to investigate muscle unloading effects on callus shape. Scx-mutant long bones had structural and mechanical defects. gene expression was elevated and was decreased at 24 h after fracture. ScxGFP cells were localized throughout the healing callus after fracture. Scx-mutant mice demonstrated disrupted callus healing and asymmetry. Asymmetry of Scx-mutant callus was not due to muscle unloading. Wild-type littermates (age matched) served as controls. This is the first study to explore the role of Scx in cortical bone mechanics and fracture healing. Deletion of Scx during development led to altered long bone properties and callus healing. This study also demonstrated that Scx may play a role in the periosteal response during fracture healing.-McKenzie, J. A., Buettmann, E., Abraham, A. C., Gardner, M. J., Silva, M. J., Killian, M. L. Loss of scleraxis in mice leads to geometric and structural changes in cortical bone, as well as asymmetry in fracture healing.
[Mh] Termos MeSH primário: Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Osso Cortical/metabolismo
Consolidação da Fratura
[Mh] Termos MeSH secundário: Animais
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética
Proteína Morfogenética Óssea 4/genética
Proteína Morfogenética Óssea 4/metabolismo
Calo Ósseo/metabolismo
Osso Cortical/lesões
Osso Cortical/fisiologia
Camundongos
Camundongos Endogâmicos C57BL
Músculo Esquelético/metabolismo
Músculo Esquelético/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Bmp4 protein, mouse); 0 (Bone Morphogenetic Protein 4); 0 (Scx protein, mouse)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE
[do] DOI:10.1096/fj.201600969R


  10 / 2098 MEDLINE  
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[PMID]:27841708
[Au] Autor:Bartnikowski N; Claes LE; Koval L; Glatt V; Bindl R; Steck R; Ignatius A; Schuetz MA; Epari DR
[Ad] Endereço:a Queensland University of Technology (QUT) , Brisbane , Australia.
[Ti] Título:Modulation of fixation stiffness from flexible to stiff in a rat model of bone healing.
[So] Source:Acta Orthop;88(2):217-222, 2017 Apr.
[Is] ISSN:1745-3682
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Background and purpose - Constant fixator stiffness for the duration of healing may not provide suitable mechanical conditions for all stages of bone repair. We therefore investigated the influence of stiffening fixation on callus stiffness and morphology in a rat diaphyseal osteotomy model to determine whether healing time was shortened and callus stiffness increased through modulation of fixation from flexible to stiff. Material and methods - An external unilateral fixator was applied to the osteotomized femur and stiffened by decreasing the offset of the inner fixator bar at 3, 7, 14, and 21 days after operation. After 5 weeks, the rats were killed and healing was evaluated with mechanical, histological, and microcomputed tomography methods. Constant fixation stiffness control groups with either stiff or flexible fixation were included for comparison. Results - The callus stiffness of the stiff group and all 4 experimental groups was greater than in the flexible group. The callus of the flexible group was larger but contained a higher proportion of unmineralized tissue and cartilage. The stiff and modulated groups (3, 7, 14, and 21 days) all showed bony bridging at 5 weeks, as well as signs of callus remodeling. Stiffening fixation at 7 and 14 days after osteotomy produced the highest degree of callus bridging. Bone mineral density in the fracture gap was highest in animals in which the fixation was stiffened after 14 days. Interpretation - The predicted benefit of a large robust callus formed through early flexible fixation could not be shown, but the benefits of stabilizing a flexible construct to achieve timely healing were demonstrated at all time points.
[Mh] Termos MeSH primário: Calo Ósseo/fisiopatologia
Fixadores Externos
Fraturas do Fêmur/cirurgia
Fêmur/fisiopatologia
Fixação de Fratura/métodos
Consolidação da Fratura
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Calo Ósseo/diagnóstico por imagem
Calo Ósseo/patologia
Diáfises
Fêmur/diagnóstico por imagem
Fêmur/patologia
Fêmur/cirurgia
Masculino
Osteotomia/métodos
Distribuição Aleatória
Ratos
Ratos Wistar
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161115
[St] Status:MEDLINE
[do] DOI:10.1080/17453674.2016.1256940



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