Base de dados : MEDLINE
Pesquisa : A10.272 [Categoria DeCS]
Referências encontradas : 66866 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 6687 ir para página                         

  1 / 66866 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:29267667
[Au] Autor:Silva LABD; Sá MAR; Melo RA; Pereira JDS; Silveira ÉJDD; Miguel MCDC
[Ad] Endereço:Universidade Federal do Rio Grande do Norte - UFRN, Postgraduate Program in Oral Pathology, Natal, RN, Brazil.
[Ti] Título:Analysis of CD57+ natural killer cells and CD8+ T lymphocytes in periapical granulomas and radicular cysts.
[So] Source:Braz Oral Res;31:e106, 2017 Dec 18.
[Is] ISSN:1807-3107
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to compare the number of CD57+ natural killer (NK) cells and CD8+ T lymphocytes between periapical granulomas (PGs) and radicular cysts (RCs). Twenty-fives cases of PGs and 25 of RCs were submitted to histological analysis and immunohistochemistry using anti-CD57 and anti-CD8 biomarkers. Positive cells were counted in 10 fields (400× magnification) and the median value was calculated for each case. Statistical tests were used to evaluate differences in the number of CD57+ NK cells and CD8+ T lymphocytes according to type of lesion, intensity of the infiltrate and thickness of the lining epithelium. The number of CD57+ NK cells and CD8+ T lymphocytes was higher in PGs than in RCs (p = 0.129 and p = 0.541, respectively). Comparison of the number of CD57+ NK cells in atrophic and hyperplastic epithelium revealed a larger number of cells in the atrophic epithelium (p = 0.042). A larger number of CD57+ NK cells and CD8+ T lymphocytes were observed in grade III infiltrates compared to grade I/II (p = 0.145 and p = 0.725, respectively). CD8+ T lymphocytes were more prevalent than CD57+ NK cells in most cases when PGs and RCs were analyzed separately or in combination (p < 0.0001). CD57+ NK cells and CD8+ T lymphocytes play a key role in antiviral defense and the presence of these cells supports evidence suggesting the participation of these microorganisms in the pathogenesis of PGs and RCs. The response mediated by CD8+ T lymphocytes was more frequent, indicating greater participation of the adaptive immunity in these chronic lesions.
[Mh] Termos MeSH primário: Antígenos CD57/análise
Linfócitos T CD8-Positivos/patologia
Células Matadoras Naturais/patologia
Granuloma Periapical/patologia
Cisto Radicular/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Biomarcadores/análise
Contagem de Células
Epitélio
Feminino
Seres Humanos
Imuno-Histoquímica
Masculino
Meia-Idade
Granuloma Periapical/imunologia
Cisto Radicular/imunologia
Valores de Referência
Índice de Gravidade de Doença
Estatísticas não Paramétricas
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (CD57 Antigens)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE


  2 / 66866 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29323924
[Au] Autor:Zhang T; Huang J; Yi Y; Zhang X; Loor JJ; Cao Y; Shi H; Luo J
[Ad] Endereço:Shanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi 712100, PR China.
[Ti] Título:Akt Serine/Threonine Kinase 1 Regulates de Novo Fatty Acid Synthesis through the Mammalian Target of Rapamycin/Sterol Regulatory Element Binding Protein 1 Axis in Dairy Goat Mammary Epithelial Cells.
[So] Source:J Agric Food Chem;66(5):1197-1205, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Akt serine/threonine kinase acts as a central mediator in the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway, regulating a series of biological processes. In lipid metabolism, Akt activation regulates a series of gene expressions, including genes related to intracellular fatty acid synthesis. However, the regulatory mechanisms of Akt in dairy goat mammary lipid metabolism have not been elaborated. In this study, the coding sequences of goat Akt1 gene were cloned and analyzed. Gene expression of Akt1 in different lactation stages was also investigated. For in vitro studies, a eukaryotic expression vector of Akt1 was constructed and transfected to goat mammary epithelial cells (GMECs), and specific inhibitors of Akt/mammalian target of rapamycin (mTOR) signaling were applied to GMECs. Results showed that Akt1 protein was highly conserved, and its mRNA was highly expressed in midlactation. In vitro studies indicated that Akt1 phosphorylation activated mTOR and subsequently enhanced sterol regulatory element binding protein 1 (SREBP1), thus increasing intracellular triacylglycerol content. Inhibition of Akt/mTOR signaling down-regulated the gene expression of lipogenic genes. Overall, Akt1 plays an important role in regulating de novo fatty acid synthesis in goat mammary epithelial cells, and this process probably is through the mTOR/SREBP1 axis.
[Mh] Termos MeSH primário: Ácidos Graxos/biossíntese
Cabras
Glândulas Mamárias Animais/metabolismo
Proteínas Proto-Oncogênicas c-akt/fisiologia
Proteína de Ligação a Elemento Regulador de Esterol 1/fisiologia
Serina-Treonina Quinases TOR/fisiologia
[Mh] Termos MeSH secundário: Animais
Epitélio/metabolismo
Regulação da Expressão Gênica/fisiologia
Lipogênese/genética
Proteínas Proto-Oncogênicas c-akt/genética
Transdução de Sinais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Sterol Regulatory Element Binding Protein 1); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05305


  3 / 66866 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29179210
[Au] Autor:Ding Y; Zhao R; Zhao X; Matthay MA; Nie HG; Ji HL
[Ad] Endereço:Institute of Metabolic Disease Research and Drug Development, China Medical University, Shenyang, China.
[Ti] Título:ENaCs as Both Effectors and Regulators of MiRNAs in Lung Epithelial Development and Regeneration.
[So] Source:Cell Physiol Biochem;44(3):1120-1132, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Epithelial sodium channels (ENaC) play an important role in re-absorbing excessive luminal fluid by building up an osmotic Na+ gradient across the tight epithelium in the airway, the lung, the kidney, and the colon. The ENaC is a major pathway for retention of salt in kidney too. MicroRNAs (miRs), a group of non-coding RNAs that regulate gene expression at the post-transcriptional level, have emerged as a novel class of regulators for ENaC. Given the ENaC pathway is crucial for maintaining fluid homeostasis in the lung and the kidney and other cavities, we summarized the cross-talk between ENaC and miRs and recapitulated the underlying regulatory factors, including aldosterone, transforming growth factor-ß1, and vascular endothelial growth factor-A in the lung and other epithelial tissues/organs. We have compared the profiling of miRs between normal and injured mice and human lungs, which showed a significant alteration in numerous miRs in mouse models of LPS and ventilator induced ARDS. In addition, we reiterated the potential regulation of the ENaC by miRs in stem/ progenitor cell-based re-epithelialization, and identified a promising pharmaceutic target of ENaC for removing edema fluid in ARDS by mesenchymal stem cells-released paracrine. In conclusion, it seems that the interactions between miRs and scnn1s/ENaCs are critical for lung development, epithelial cell turnover in adult lungs, and re-epithelialization for repair.
[Mh] Termos MeSH primário: Canais Epiteliais de Sódio/metabolismo
Epitélio/fisiologia
Pulmão/fisiologia
MicroRNAs/metabolismo
[Mh] Termos MeSH secundário: Animais
Epitélio/crescimento & desenvolvimento
Seres Humanos
Regeneração
Células-Tronco/citologia
Células-Tronco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Epithelial Sodium Channels); 0 (MicroRNAs)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180203
[Lr] Data última revisão:
180203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1159/000485417


  4 / 66866 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28743797
[Au] Autor:Castillo-Azofeifa D; Losacco JT; Salcedo E; Golden EJ; Finger TE; Barlow LA
[Ad] Endereço:Department of Cell and Developmental Biology and the Rocky Mountain Taste and Smell Center University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA.
[Ti] Título:Sonic hedgehog from both nerves and epithelium is a key trophic factor for taste bud maintenance.
[So] Source:Development;144(17):3054-3065, 2017 09 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The integrity of taste buds is intimately dependent on an intact gustatory innervation, yet the molecular nature of this dependency is unknown. Here, we show that differentiation of new taste bud cells, but not progenitor proliferation, is interrupted in mice treated with a hedgehog (Hh) pathway inhibitor (HPI), and that gustatory nerves are a source of sonic hedgehog (Shh) for taste bud renewal. Additionally, epithelial taste precursor cells express Shh transiently, and provide a local supply of Hh ligand that supports taste cell renewal. Taste buds are minimally affected when Shh is lost from either tissue source. However, when both the epithelial and neural supply of Shh are removed, taste buds largely disappear. We conclude Shh supplied by taste nerves and local taste epithelium act in concert to support continued taste bud differentiation. However, although neurally derived Shh is in part responsible for the dependence of taste cell renewal on gustatory innervation, neurotrophic support of taste buds likely involves a complex set of factors.
[Mh] Termos MeSH primário: Epitélio/inervação
Epitélio/metabolismo
Proteínas Hedgehog/metabolismo
Papilas Gustativas/metabolismo
[Mh] Termos MeSH secundário: Animais
Proliferação Celular
Tamanho Celular
Feminino
Deleção de Genes
Masculino
Camundongos
Células Receptoras Sensoriais/metabolismo
Transdução de Sinais
Células-Tronco/citologia
Células-Tronco/metabolismo
Paladar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Hedgehog Proteins); 0 (Shh protein, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1242/dev.150342


  5 / 66866 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29176774
[Au] Autor:Viktorinová I; Henry I; Tomancak P
[Ad] Endereço:Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
[Ti] Título:Epithelial rotation is preceded by planar symmetry breaking of actomyosin and protects epithelial tissue from cell deformations.
[So] Source:PLoS Genet;13(11):e1007107, 2017 Nov.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Symmetry breaking is involved in many developmental processes that form bodies and organs. One of them is the epithelial rotation of developing tubular and acinar organs. However, how epithelial cells move, how they break symmetry to define their common direction, and what function rotational epithelial motions have remains elusive. Here, we identify a dynamic actomyosin network that breaks symmetry at the basal surface of the Drosophila follicle epithelium of acinar-like primitive organs, called egg chambers, and may represent a candidate force-generation mechanism that underlies the unidirectional motion of this epithelial tissue. We provide evidence that the atypical cadherin Fat2, a key planar cell polarity regulator in Drosophila oogenesis, directs and orchestrates transmission of the intracellular actomyosin asymmetry cue onto a tissue plane in order to break planar actomyosin symmetry, facilitate epithelial rotation in the opposite direction, and direct the elongation of follicle cells. In contrast, loss of this rotational motion results in anisotropic non-muscle Myosin II pulses that are disorganized in plane and causes cell deformations in the epithelial tissue of Drosophila eggs. Our work demonstrates that atypical cadherins play an important role in the control of symmetry breaking of cellular mechanics in order to facilitate tissue motion and model epithelial tissue. We propose that their functions may be evolutionarily conserved in tubular/acinar vertebrate organs.
[Mh] Termos MeSH primário: Actomiosina/metabolismo
Drosophila melanogaster/metabolismo
Células Epiteliais/metabolismo
Epitélio/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Caderinas/genética
Caderinas/metabolismo
Movimento Celular
Proteínas de Drosophila/genética
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/genética
Feminino
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Microscopia Confocal
Cadeias Leves de Miosina/genética
Cadeias Leves de Miosina/metabolismo
Ovário/citologia
Ovário/metabolismo
Óvulo/metabolismo
Rotação
Imagem com Lapso de Tempo/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cadherins); 0 (Drosophila Proteins); 0 (Fat2 protein, Drosophila); 0 (Myosin Light Chains); 147336-22-9 (Green Fluorescent Proteins); 9013-26-7 (Actomyosin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007107


  6 / 66866 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29187496
[Au] Autor:Shibata H; Ohike N; Norose T; Isobe T; Suzuki R; Imai H; Shiokawa A; Aoki T; Murakami M; Mizukami H; Tanaka JI; Takimoto M
[Ad] Endereço:Department of Pathology, Showa University Hospital, Tokyo, Japan hshibata@med.showa-u.ac.jp.
[Ti] Título:Mucinous Cystic Neoplasms Lined by Abundant Mucinous Epithelium Frequently Involve KRAS Mutations and Malignant Progression.
[So] Source:Anticancer Res;37(12):7063-7068, 2017 12.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Pancreatic and hepatic mucinous cyst neoplasms (MCNs) have a malignant potential, but indolent MCNs are not uncommon. MATERIALS AND METHODS: The pathological and genetic characteristics of resected MCNs (n=15) categorized by the amount of mucin of the lining epithelium were investigated. RESULTS: MCNs were divided into two groups: (i) a rich (r)-MCN group (n=6), in which more than half of the epithelium was lined by abundant mucinous epithelium; and (ii) a poor (p)-MCN group (n=9), which consisted of the remaining cases. Three patients in the r-MCN group showed invasive carcinoma or high-grade dysplasia, whereas all patients in the p-MCN group showed low-grade dysplasia. Mutations of Kirsten rat sarcoma viral oncogene homolog (KRAS) were more frequent in the r-MCN group (83%) (p-MCN; 11%, p<0.05). CONCLUSION: Mucinous MCNs more frequently have KRAS mutations and higher risk of malignant progression.
[Mh] Termos MeSH primário: Epitélio/metabolismo
Neoplasias Hepáticas/genética
Mutação
Neoplasias Císticas, Mucinosas e Serosas/genética
Neoplasias Pancreáticas/genética
Proteínas Proto-Oncogênicas p21(ras)/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Progressão da Doença
Epitélio/patologia
Feminino
Seres Humanos
Neoplasias Hepáticas/metabolismo
Neoplasias Hepáticas/patologia
Masculino
Meia-Idade
Mucinas/metabolismo
Neoplasias Císticas, Mucinosas e Serosas/metabolismo
Neoplasias Císticas, Mucinosas e Serosas/patologia
Neoplasias Pancreáticas/metabolismo
Neoplasias Pancreáticas/patologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (KRAS protein, human); 0 (Mucins); EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE


  7 / 66866 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29180196
[Au] Autor:Marshall JK; Tait N; van der Linden J
[Ad] Endereço:Illawarra Health and Medical Institute, University of Wollongong, Wollongong, Australia. Electronic address: jeankmarshall@gmail.com.
[Ti] Título:Laparotomy causes loss of peritoneal mesothelium prevented by humidified CO insufflation in rats.
[So] Source:J Surg Res;220:300-310, 2017 Dec.
[Is] ISSN:1095-8673
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Avoiding tissue desiccation is a common recommendation to reduce postoperative complications following open abdominal surgery, although difficult to achieve delicately without damaging the peritoneal mesothelium. Insufflation of humidified-warm CO into the abdomen during open abdominal surgery is proposed as an invisible, effortless way to prevent desiccation. We hypothesized that desiccation during open abdominal surgery would cause loss of peritoneal mesothelium that would be prevented by insufflation of humidified-warm CO . METHODS: Nine Wistar rats were assigned to 1 h of anesthesia only, laparotomy only, or laparotomy with insufflation of humidified-warm CO . Twelve hours after treatment, rats were euthanized and tissue samples were excised. Scanning electron microscopy (SEM) and light microscopy (LM) images of visceral and parietal peritoneum were scored by two independent, blinded examiners for loss of mesothelium and other indications of inflammation, including measurement of apoptosis by detection of DNA cleavage. RESULTS: Loss of peritoneal mesothelium was found in peritoneum exposed to laparotomy only (SEM: P = 0.002; LM: P = 0.01), and mesothelial loss was reduced by humidified-warm CO (SEM: P < 0.001; LM P = 0.004). Similarly, DNA cleavage was significantly higher on the peritoneal surface following laparotomy only, compared with anesthesia only (P = 0.0055) and laparotomy with humidified-warm CO insufflation (P = 0.0003). CONCLUSIONS: In a rat model, exposing the peritoneal mesothelial to conditions that replicate minimum recommended air flow within an operating room causes inadvertent loss of mesothelium and signs of inflammation that can be prevented by insufflating humidified-warm CO into the open abdominal cavity.
[Mh] Termos MeSH primário: Dióxido de Carbono/uso terapêutico
Insuflação/métodos
Laparotomia/efeitos adversos
Doenças Peritoneais/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Epitélio/patologia
Feminino
Doenças Peritoneais/etiologia
Doenças Peritoneais/patologia
Ratos Wistar
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


  8 / 66866 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28745321
[Au] Autor:Andò S; Malivindi R; Catalano S; Rizza P; Barone I; Panza S; Rovito D; Emprou C; Bornert JM; Laverny G; Metzger D
[Ad] Endereço:Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, Arcavacata di Rende (CS), Italy.
[Ti] Título:Conditional expression of Ki-Ras in the mammary epithelium of transgenic mice induces estrogen receptor alpha (ERα)-positive adenocarcinoma.
[So] Source:Oncogene;36(46):6420-6431, 2017 Nov 16.
[Is] ISSN:1476-5594
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Appropriate 'in vivo' models are crucial for studying breast cancer biology and evaluating the efficacy of therapeutic agents. Thus we engineered a novel transgenic mouse line expressing the human Ki-Ras bearing an activating mutation (Ki-Ras ) selectively in the mammary epithelium after lactation. These mice develop invasive ductal adenocarcinomas with 100% incidence within 3-9 months after Ki-Ras induction. Immunophenotyping revealed that the mammary tumors express luminal markers, are positive for estrogen and progesterone receptors, negative for HER2 and have a low proliferation index. Moreover, cell lines derived from such tumors are estrogen-responsive and, when transplanted into nude mice, form tumors that respond to the antiestrogen ICI 182780. In conclusion, the mammary tumors of these transgenic mice and the derived cell lines exhibit key features of the major form of human breast cancer, that is, luminal A subtype and thus have a high potential for breast cancer research and treatment.
[Mh] Termos MeSH primário: Adenocarcinoma/genética
Epitélio/metabolismo
Receptor alfa de Estrogênio/genética
Genes ras/genética
Glândulas Mamárias Animais/metabolismo
Neoplasias Mamárias Experimentais/genética
[Mh] Termos MeSH secundário: Adenocarcinoma/metabolismo
Animais
Estradiol/análogos & derivados
Estradiol/farmacologia
Antagonistas do Receptor de Estrogênio/farmacologia
Receptor alfa de Estrogênio/metabolismo
Feminino
Expressão Gênica
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Células MCF-7
Neoplasias Mamárias Experimentais/tratamento farmacológico
Neoplasias Mamárias Experimentais/metabolismo
Camundongos Endogâmicos C57BL
Camundongos Nus
Camundongos Transgênicos
Mutação de Sentido Incorreto
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estrogen Receptor Antagonists); 0 (Estrogen Receptor alpha); 22X328QOC4 (fulvestrant); 4TI98Z838E (Estradiol)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1038/onc.2017.252


  9 / 66866 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28743800
[Au] Autor:Ghosh A; Syed SM; Tanwar PS
[Ad] Endereço:Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, New South Wales, 2308, Australia.
[Ti] Título: genetic cell lineage tracing reveals that oviductal secretory cells self-renew and give rise to ciliated cells.
[So] Source:Development;144(17):3031-3041, 2017 09 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The epithelial lining of the fallopian tube is vital for fertility, providing nutrition to gametes and facilitating their transport. It is composed of two major cell types: secretory cells and ciliated cells. Interestingly, human ovarian cancer precursor lesions primarily consist of secretory cells. It is unclear why secretory cells are the dominant cell type in these lesions. Additionally, the underlying mechanisms governing fallopian tube epithelial homoeostasis are unknown. In the present study, we showed that across the different developmental stages of mouse oviduct, secretory cells are the most frequently dividing cells of the oviductal epithelium. genetic cell lineage tracing showed that secretory cells not only self-renew, but also give rise to ciliated cells. Analysis of a Wnt reporter mouse model and various Wnt target genes showed that the Wnt signaling pathway is involved in oviductal epithelial homoeostasis. By developing two triple-transgenic mouse models, we showed that Wnt/ß-catenin signaling is essential for self-renewal as well as the differentiation of secretory cells. In summary, our results provide mechanistic insight into oviductal epithelial homoeostasis.
[Mh] Termos MeSH primário: Linhagem da Célula
Autorrenovação Celular
Rastreamento de Células
Cílios/metabolismo
Oviductos/citologia
Oviductos/secreção
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Proliferação Celular
Epitélio/patologia
Tubas Uterinas/patologia
Feminino
Predisposição Genética para Doença
Seres Humanos
Camundongos Endogâmicos C57BL
Neoplasias Ovarianas/patologia
Fator de Transcrição PAX8/metabolismo
Fatores de Risco
Via de Sinalização Wnt
beta Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (PAX8 Transcription Factor); 0 (Pax8 protein, mouse); 0 (beta Catenin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1242/dev.149989


  10 / 66866 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29060967
[Au] Autor:Mao YN; Zeng LX; Li YH; Liu YZ; Wu JY; Li L; Wang Q
[Ad] Endereço:Laboratory of Early Prevention and Treatment for Regional High Frequence Tumor Ministry of Education Key Laboratory, Affiliated Tumor Hospital, Guangxi Medical University, Nanning 530021, China.
[Ti] Título:[Significance and expression of PAX8, PAX2, p53 and RAS in ovary and fallopian tubes to origin of ovarian high grade serous carcinoma].
[So] Source:Zhonghua Fu Chan Ke Za Zhi;52(10):687-696, 2017 Oct 25.
[Is] ISSN:0529-567X
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To explore the origin of ovarian high grade serous carcinoma (HGSC) through analysing the expression and significance of PAX8, PAX2, p53 and RAS in the ovary and fallopian tube of different types and grades of serous carcinoma. A total of 44 cases tissue samples of ovarian tumor including 34 malignant ovarian tumor and 10 normal normal tissue (as control group) were collected from the admitted patients in Affiliated Tumor Hospital of Guangxi Medical University from January 2015 to January 2016. Fallopian tube tissues were segmented in accordance with the fimbria, ampulla, isthmus and the corresponding ovarian tissues were by the side. There were 34 cases of patients with ovarian cancer including 29 cases of epithelial ovarian cancer (27 serous carcinoma, 1 mucinous carcinoma,1 endometrioid adenocarcinoma) and 5 non-epithelial ovarian cancer (sex cord-interstitial tumor). Among 27 cases of patients with ovarian serous cancer, there were 23 HGSC and 4 low-grade ovarian serous cancer (LGSC). One hundred fifty-three cases of samples were diagnosed as ovarian serous cancer by Shandong University Affiliated Qilu Hospital from 2005 to 2013 and these samples were made tissue microarray. (1) To analyze the expression and differences of PAX8, PAX2, p53 and RAS in the above tissues and tissue microarray from ovarian and tubal of HGSC and control women by immunohistochemistry methods. (2) To compare the expression levels of PAX8, PAX2, p53 and RAS in ovarian and fallopian tubes of ovarian cancer patients with different pathological types. (3) To analyze the correlations of tubal and ovarian tissue in PAX8, PAX2, p53 and RAS expression of HGSC. (4) To analyze the factors of the prognosis of ovarian serous cancer in tissue microarray by single factor analysis method. (1) PAX8, PAX2, p53 and RAS expression was negative in normal ovarian epithelium of control group, but the expression of PAX8, PAX2, p53 and RAS were strongly positive brown in secrete cells of normal fallopian tube epithelium. (2) p53 and RAS expression of fallopian tube epithelium in the epithelial ovarian cancer group were significantly higher than those in the non-epithelial ovarian cancer groups ( 0.05), but the expression of PAX8 and PAX2 in fallopian tube and the expression of PAX8, PAX2, p53 and RAS in ovarian tissue was not statistically significant in the groups ( 0.05). PAX8, PAX2 and p53 expression of the ovarian in HGSC group were significantly higher than those in LGSC group ( 0.05), while the expression of RAS was lower in the ovarian of the high-grade group ( 0.05), while the expression of PAX8, PAX2, p53 and RAS in fallopian tube was not statistically significant in the groups ( 0.05). (3) There was a significantly positive correlation between fallopian tube and the corresponding ovary of HGSC in PAX8 and PAX2 expression ( 0.422, 0.045; 0.693, 0.000), but not correlation in p53 and RAS expression ( 0.058, 0.793; 0.190, 0.384). (4) Univariate survival analysis showed that the progression free survival time in patients with ovarian serous cancer group was significantly correlated with the protein expression of PAX8, PAX2 and RAS ( 0.05), but there were not correlated with age, surgical staging, cell differentiation, lymph node metastasis and preoperative chemotherapy and p53 protein expression ( 0.05). The total survival time in patients with ovarian serous cancer group was significantly correlated with the protein expression of PAX8 ( 0.05), but there were not correlated with age,surgical staging, cell differentiation, lymph node metastasis and preoperative chemotherapy and the protein expression of PAX2, RAS and p53 ( 0.05). PAX8, PAX2, p53, RAS are of great significance for the study of origin of HGSC. HGSC may be derived from fallopian tube, but further investigation would be necessary to confirm this. PAX8, PAX2, p53, RAS could be expected to be used as predictors of survival prognosis in patients with ovarian serous cancer.
[Mh] Termos MeSH primário: Cistadenocarcinoma Seroso/patologia
Neoplasias das Tubas Uterinas/patologia
Tubas Uterinas/patologia
Proteínas Monoméricas de Ligação ao GTP/metabolismo
Neoplasias Epiteliais e Glandulares/patologia
Neoplasias Ovarianas/patologia
Fator de Transcrição PAX2/metabolismo
Fator de Transcrição PAX8/metabolismo
Proteína Supressora de Tumor p53/metabolismo
[Mh] Termos MeSH secundário: Carcinoma Endometrioide
China
Cistadenocarcinoma Seroso/genética
Cistadenocarcinoma Seroso/metabolismo
Epitélio
Neoplasias das Tubas Uterinas/genética
Neoplasias das Tubas Uterinas/metabolismo
Tubas Uterinas/metabolismo
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Imuno-Histoquímica
Proteínas Monoméricas de Ligação ao GTP/genética
Proteínas de Neoplasias/genética
Proteínas de Neoplasias/metabolismo
Neoplasias Epiteliais e Glandulares/genética
Neoplasias Epiteliais e Glandulares/metabolismo
Neoplasias Ovarianas/genética
Neoplasias Ovarianas/metabolismo
Fator de Transcrição PAX2/genética
Fator de Transcrição PAX8/genética
Proteína Supressora de Tumor p53/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neoplasm Proteins); 0 (PAX2 Transcription Factor); 0 (PAX2 protein, human); 0 (PAX8 Transcription Factor); 0 (PAX8 protein, human); 0 (Tumor Suppressor Protein p53); EC 3.6.5.2 (Monomeric GTP-Binding Proteins); EC 3.6.5.2 (REM2 protein, human)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171025
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-567X.2017.10.008



página 1 de 6687 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde