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[PMID]: 29458473 [Au] Autor: Lee DG; Trujillo ME; Kang S; Nam JJ; Kim YJ [Ad] Endereço: COSMAX R&I center, Republic of Korea. [Ti] Título: Epidermidibacterium keratini gen. nov., sp. nov., a member of the family Sporichthyaceae, isolated from keratin epidermis. [So] Source: Int J Syst Evol Microbiol;68(3):745-750, 2018 Mar. [Is] ISSN: 1466-5034 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A novel actinobacterial strain, designated EPI-7 , was isolated on R2A agar from human skin (keratinocytes) and subjected to a taxonomic study using a polyphasic approach. Strain EPI-7 showed a Gram-positive reaction, was non-motile, non-spore-forming, and cells had a rod-shape. Colonies were round, convex and pale yellow. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya. The 16S rRNA gene sequence similarities with respect to the type strains of recognized species from the above genera and other phylogenetic neighbours ranged from 92.6 to 93.4 %. The G+C content of the genomic DNA was 68.9 mol%. The only isoprenoid quinone was MK-9(H4), and the major fatty acids detected were C17 : 1ω8c, C16 : 0, iso-C15 : 0 and summed feature 3. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids, phosphatidylglycerol, phosphatidylcholine, two unidentified amino lipids and three unidentified lipids. The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine. Whole-cell sugars present included rhamnose, glucose and galactose. The combination of the genotypic and phenotypic data allowed differentiation of strain EPI-7 from its closest phylogenetic neighbours and provided evidence that strain EPI-7 represents a novel genus and species in the family Sporichthyaceae. The name Epidermidibacterium keratini gen. nov., sp. nov. is proposed with the type strain being EPI-7 (=KCCM 90264 =JCM 31644 ). [Mh] Termos MeSH primário: Actinomycetales/classificação Epiderme/microbiologia Queratinócitos/microbiologia Filogenia [Mh] Termos MeSH secundário: Actinomycetales/genética Actinomycetales/isolamento & purificação Técnicas de Tipagem Bacteriana Composição de Bases DNA Bacteriano/genética Ácido Diaminopimélico/química Ácidos Graxos/química Seres Humanos Peptidoglicano/química Fosfolipídeos/química Pigmentação RNA Ribossômico 16S/genética República da Coreia Análise de Sequência de DNA Vitamina K 2/análogos & derivados Vitamina K 2/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-39-7 (menaquinone 9); 583-93-7 (Diaminopimelic Acid) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180308 [Lr] Data última revisão: 180308 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180221 [St] Status: MEDLINE [do] DOI: 10.1099/ijsem.0.002579

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[PMID]: 29374927 [Au] Autor: You X; Wei ZR [Ad] Endereço: Department of Plastic Surgery, the Affiliated Hospital of Zunyi Medical College, Zunyi, 563000, China. [Ti] Título: [Advances in the research of function of Merkel cells in tactile formation of skin]. [So] Source: Zhonghua Shao Shang Za Zhi;34(1):51-54, 2018 Jan 20. [Is] ISSN: 1009-2587 [Cp] País de publicação: China [La] Idioma: chi [Ab] Resumo: Skin is the largest sense organ of human, with many mechanoreceptor cells under epidermis or dermis of skin and Merkel cell is one of them. It has been confirmed that Merkel cells play an important role in the process of mechanical transmission of mammalian soft tactile stimulation. Researches showed that Merkel cells had close relation to tactile formation and functioned by Merkel cell-neurite complexes and ion channels Piezo2. This article reviews Merkel cells and the function, problem and prospect of Merkel cells in tactile formation. [Mh] Termos MeSH primário: Células de Merkel/fisiologia Tato/fisiologia [Mh] Termos MeSH secundário: Animais Epiderme Seres Humanos Canais Iônicos Mecanorreceptores Pele [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Ion Channels) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180306 [Lr] Data última revisão: 180306 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180129 [St] Status: MEDLINE [do] DOI: 10.3760/cma.j.issn.1009-2587.2018.01.010

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[PMID]: 29184403 [Au] Autor: Zou Y; Celli A; Zhu H; Elmahdy A; Cao Y; Hui X; Maibach H [Ad] Endereço: Skin & Cosmetic Research Department, Shanghai Skin Disease Hospital, Shanghai, People's Republic of China. [Ti] Título: Confocal laser scanning microscopy to estimate nanoparticles' human skin penetration in vitro. [So] Source: Int J Nanomedicine;12:8035-8041, 2017. [Is] ISSN: 1178-2013 [Cp] País de publicação: New Zealand [La] Idioma: eng [Ab] Resumo: Objective: With rapid development of nanotechnology, there is increasing interest in nanoparticle (NP) application and its safety and efficacy on human skin. In this study, we utilized confocal laser scanning microscopy to estimate NP skin penetration. Methods: Three different-sized polystyrene NPs marked with red fluorescence were applied to human skin, and Calcium Green 5N was used as a counterstain. Dimethyl sulfoxide (DMSO) and ethanol were used as alternative vehicles for NPs. Tape stripping was utilized as a barrier-damaged skin model. Skin biopsies dosed with NPs were incubated at 4°C or 37°C for 24 hours and imaged using confocal laser scanning microscopy. Results: NPs were localized in the stratum corneum (SC) and hair follicles without penetrating the epidermis/dermis. Barrier alteration with tape stripping and change in incubation temperature did not induce deeper penetration. DMSO enhanced NP SC penetration but ethanol did not. Conclusion: Except with DMSO vehicle, these hydrolyzed polystyrene NPs did not penetrate intact or barrier-damaged human "viable" epidermis. For further clinical relevance, in vivo human skin studies and more sensitive analytic chemical methodology are suggested. [Mh] Termos MeSH primário: Microscopia Confocal/métodos Nanopartículas Absorção Cutânea/efeitos dos fármacos [Mh] Termos MeSH secundário: Epiderme/efeitos dos fármacos Seres Humanos Nanopartículas/administração & dosagem Nanopartículas/química Compostos Orgânicos/administração & dosagem Compostos Orgânicos/farmacocinética Tamanho da Partícula Pele/efeitos dos fármacos Temperatura Ambiente [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Organic Chemicals); 138067-55-7 (calcium green) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180305 [Lr] Data última revisão: 180305 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171130 [St] Status: MEDLINE [do] DOI: 10.2147/IJN.S139139

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[PMID]: 29382339 [Au] Autor: Lee HJ; Im AR; Kim SM; Kang HS; Lee JD; Chae S [Ad] Endereço: Department of Microbiology, Disivion of Natural Science, Pusan National University, Busan, 609-735, South Korea. [Ti] Título: The flavonoid hesperidin exerts anti-photoaging effect by downregulating matrix metalloproteinase (MMP)-9 expression via mitogen activated protein kinase (MAPK)-dependent signaling pathways. [So] Source: BMC Complement Altern Med;18(1):39, 2018 Jan 30. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Hesperidin is a flavonoid with antioxidant, anti-inflammatory, and immune modulatory activities. Photoaging is a consequence of chronic exposure to the sun and ultraviolet (UV) radiation. This study was designed to evaluate the efficacy of hesperidin against photoaging of dorsal skin in hairless mice. METHODS: Hairless male mice (6-week-old) were divided into three groups (n = 7): control, UVB-treated vehicle, and UVB-treated hesperidin groups. UVB-irradiated mice from hesperidin group were orally administered 0.1 mL of water containing 100 mg/kg body weight per day hesperidin. RESULTS: The mean length and depth of wrinkles in the UVB-treated hesperidin group significantly improved after the oral administration of hesperidin, which significantly inhibited the increase in epidermal thickness and epidermal hypertrophy (P < 0.05). UVB irradiation of mice induced epidermal barrier dysfunction including an increase in the transepidermal water loss (TEWL); however, hesperidin decreased the TEWL. UVB irradiation increased the expression of MMP-9 and pro-inflammatory cytokines whereas UVB-treated hesperidin group showed reduced expression. These results indicate that hesperidin showed anti-photoaging activity in the UVB-irradiated hairless mice. In conclusion, hesperidin inhibited the UVB-induced increase in skin thickness, wrinkle formation, and collagen fiber loss in male hairless mice. CONCLUSIONS: These results suggest that hesperidin shows potent anti-photoaging activity by regulating MMP-9 expression through the suppression of MAPK-dependent signaling pathways. [Mh] Termos MeSH primário: Antioxidantes/farmacologia Hesperidina/farmacologia Sistema de Sinalização das MAP Quinases/efeitos dos fármacos Metaloproteinase 9 da Matriz/metabolismo Envelhecimento da Pele/efeitos dos fármacos [Mh] Termos MeSH secundário: Animais Regulação para Baixo/efeitos dos fármacos Epiderme/efeitos dos fármacos Epiderme/efeitos da radiação Imuno-Histoquímica Masculino Camundongos Camundongos Pelados Envelhecimento da Pele/efeitos da radiação Raios Ultravioleta [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antioxidants); E750O06Y6O (Hesperidin); EC 3.4.24.35 (Matrix Metalloproteinase 9) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180301 [Lr] Data última revisão: 180301 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180201 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-017-2058-8

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[PMID]: 29339067 [Au] Autor: Sun Y; Zhang J; Xiang J [Ad] Endereço: College of Life Sciences, Hebei University, Baoding, Hebei 071002, China; College of Marine Life and Fisheries, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China. [Ti] Título: Molecular characterization and function of ß-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda. [So] Source: Gene;648:12-20, 2018 Mar 30. [Is] ISSN: 1879-0038 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Chitin degradation is catalyzed by a two-component chitinolytic enzyme system, chitinase and ß-N-acetylglucosaminidase (NAGase). In this paper, the full-length cDNA sequence encoding NAGase (EcNAG) was obtained from Exopalaemon carinicauda. The deduced amino acid sequence of EcNAG open reading frame (ORF) contained one Glycohydro_20b2 domain and one Glyco_hydro_20 domain. Based on the cDNA sequence, the genomic structure of EcNAG was characterized and it was composed of six exons and five introns. EcNAG mRNA majorly expressed in the hepatopancreas and epidermis. During the molting stages, EcNAG mRNA expression was well-regulated and its expression reached the highest level at the molting stage E. In addition, EcNAG was recombinant expressed in Pichia pastoris and the partial enzymatic characterization of recombinant EcNAG was confirmed. After being challenged with Vibrio parahaemolyticus and Aeromonas hydrophila, the expression of EcNAG was up-regulated significantly at 6 h and reached the peak at 12 h. And then, the expression began to down-regulated and came to the normal level at 72 h. It is helpful to research the relationship between the molt-related hormones and chitinlytic enzymes. [Mh] Termos MeSH primário: Acetilglucosaminidase/genética Proteínas de Artrópodes/genética Muda/genética Palaemonidae/genética [Mh] Termos MeSH secundário: Acetilglucosaminidase/classificação Acetilglucosaminidase/metabolismo Aeromonas hydrophila/fisiologia Sequência de Aminoácidos Animais Proteínas de Artrópodes/classificação Proteínas de Artrópodes/metabolismo Sequência de Bases Epiderme/crescimento & desenvolvimento Epiderme/metabolismo Epiderme/microbiologia Doenças dos Peixes/genética Doenças dos Peixes/microbiologia Perfilação da Expressão Gênica/métodos Regulação da Expressão Gênica no Desenvolvimento Regulação Enzimológica da Expressão Gênica Hepatopâncreas/crescimento & desenvolvimento Hepatopâncreas/metabolismo Hepatopâncreas/microbiologia Palaemonidae/crescimento & desenvolvimento Palaemonidae/microbiologia Filogenia Homologia de Sequência de Aminoácidos Vibrio parahaemolyticus/fisiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Arthropod Proteins); EC 3.2.1.52 (Acetylglucosaminidase) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180226 [Lr] Data última revisão: 180226 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180118 [St] Status: MEDLINE

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[PMID]: 28745643 [Au] Autor: Li F; Adase CA; Zhang LJ [Ad] Endereço: Department of Dermatology, School of Medicine, UC San Diego. [Ti] Título: Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin. [So] Source: J Vis Exp;(125), 2017 Jul 14. [Is] ISSN: 1940-087X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The keratinocyte (KC) is the predominant cell type in the epidermis, the outermost layer of the skin. Epidermal KCs play a critical role in providing skin defense by forming an intact skin barrier against environmental insults, such as UVB irradiation or pathogens, and also by initiating an inflammatory response upon those insults. Here we describe methods to isolate KCs from neonatal mouse skin and from adult mouse tail skin. We also describe culturing conditions using defined growth supplements (dGS) in comparison to chelexed fetal bovine serum (cFBS). Functionally, we show that both neonatal and adult KCs are highly responsive to high calcium-induced terminal differentiation, tight junction formation and stratification. Additionally, cultured adult KCs are susceptible to UVB-triggered cell death and can release large amounts of TNF upon UVB irradiation. Together, the methods described here will be useful to researchers for the setup of in vitro models to study epidermal biology in the neonatal mouse and/or the adult mouse. [Mh] Termos MeSH primário: Queratinócitos/citologia Pele/citologia [Mh] Termos MeSH secundário: Animais Animais Recém-Nascidos Apoptose/efeitos da radiação Cálcio/farmacologia Diferenciação Celular/efeitos dos fármacos Células Cultivadas Epiderme/citologia Queratinócitos/metabolismo Queratinócitos/efeitos da radiação Camundongos Fator de Necrose Tumoral alfa/metabolismo Raios Ultravioleta Gravação em Vídeo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Tumor Necrosis Factor-alpha); SY7Q814VUP (Calcium) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180227 [Lr] Data última revisão: 180227 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170727 [St] Status: MEDLINE [do] DOI: 10.3791/56027

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[PMID]: 29413990 [Au] Autor: Kelly J; Murphy JE [Ad] Endereço: Mitochondrial Biology & Radiation Research Centre, Dept. of Life Sciences, Institute of Technology Sligo, Ash Lane, Sligo, Ireland. Electronic address: janiskelly@mail.itsligo.ie. [Ti] Título: Mitochondrial gene expression changes in cultured human skin cells following simulated sunlight irradiation. [So] Source: J Photochem Photobiol B;179:167-174, 2018 Feb. [Is] ISSN: 1873-2682 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: BACKGROUND: Exposure of skin to simulated sunlight irradiation (SSI) has being extensively researched and shown to be the main cause for changes in the skin including changes in cellular function and generation of reactive oxygen species (ROS). This oxidative stress can subsequently exert downstream effects and the subcellular compartments most affected by this oxidative stress are mitochondria. The importance of functional mitochondrial morphology is apparent as morphological defects are related to many human diseases including diabetes mellitus, liver disease, neurodegenerative diseases, aging and cancer. OBJECTIVE: The main objective of this study was to evaluate solar radiation-induced changes in mitochondrial gene expression in human skin cells using a Q-Sun solar simulator to deliver a close match to the intensity of summer sunlight. METHODS: Spontaneously immortalised human skin epidermal keratinocytes (HaCaT) and Human Dermal Fibroblasts (HDFn) were divided into two groups. Group A were irradiated once and Group B twice 7days apart; following irradiation, mitochondrial gene expression was evaluated 1, 4 and 7days post primary exposure for group A and 1, 4, 7 and 14days post-secondary exposure for group B. RESULTS: Both the epidermal and dermal cells displayed significant reduced expression of the genes analysed for mitochondrial morphology and function; however, epidermal cells displayed this reduction post SSI earlier then dermal cells at multiple time points. CONCLUSION: The data presented here reinforces the fact that epidermal cells, while displaying a heightened sensitivity to sunlight, are less prone to changes in gene expression, while dermal cells, which appear to be more resilient are possibly more prone to genomic instability and mitochondrial damage. [Mh] Termos MeSH primário: Expressão Gênica/efeitos da radiação Mitocôndrias/genética Luz Solar [Mh] Termos MeSH secundário: ATPases Associadas a Diversas Atividades Celulares/genética ATPases Associadas a Diversas Atividades Celulares/metabolismo Linhagem Celular Derme/citologia Epiderme/citologia GTP Fosfo-Hidrolases/genética GTP Fosfo-Hidrolases/metabolismo Seres Humanos Queratinócitos/citologia Queratinócitos/metabolismo Queratinócitos/efeitos da radiação Metaloendopeptidases/genética Metaloendopeptidases/metabolismo Mitocôndrias/efeitos da radiação Proteínas de Transporte da Membrana Mitocondrial/genética Proteínas de Transporte da Membrana Mitocondrial/metabolismo Proteínas Mitocondriais/genética Proteínas Mitocondriais/metabolismo Espécies Reativas de Oxigênio/metabolismo Reação em Cadeia da Polimerase Via Transcriptase Reversa Superóxido Dismutase/genética Superóxido Dismutase/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Mitochondrial Membrane Transport Proteins); 0 (Mitochondrial Proteins); 0 (Reactive Oxygen Species); EC 1.15.1.1 (Superoxide Dismutase); EC 1.15.1.1 (superoxide dismutase 2); EC 3.4.24.- (Metalloendopeptidases); EC 3.4.24.- (YME1L1 protein, human); EC 3.6.1.- (GTP Phosphohydrolases); EC 3.6.1.- (MFN2 protein, human); EC 3.6.1.- (OPA1 protein, human); EC 3.6.4.- (ATPases Associated with Diverse Cellular Activities); EC 3.6.5.- (Mfn1 protein, human) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180223 [Lr] Data última revisão: 180223 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180208 [St] Status: MEDLINE

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[PMID]: 29406161 [Au] Autor: Voronin VN; Golineva EA; Dudin AS [Ti] Título: [Henneguya wolinensis (Myxosporea: Myxobolidae), a new for Russian fauna parasite from the perch Perca fluviatilis L.]. [So] Source: Parazitologiia;51(2):165-9, 2017 Mar-Apr. [Is] ISSN: 0031-1847 [Cp] País de publicação: Russia (Federation) [La] Idioma: rus [Ab] Resumo: The infection of the perch Perea fluviatilis L. with myxosporean Henneguya wolinensis Romuk-Wodoracki, 1990 has been detected. This is the second finding of this parasite after its original descriptin and the first for Russia. Plasmodium of this species develops in the epidermis under scales throughout the body causing the formation of white cysts up to 1 mm. Spores are fusiform, large, their average length constitutes 25.5 µm without the caudal appendages and 62 µm with them. Slight morphological differences in spore structure comparing to original description have been revealed. [Mh] Termos MeSH primário: Cistos/patologia Epiderme/parasitologia Myxozoa/fisiologia Percas/parasitologia Esporos/fisiologia [Mh] Termos MeSH secundário: Animais Epiderme/patologia Doenças dos Peixes/epidemiologia Doenças dos Peixes/parasitologia Myxozoa/anatomia & histologia Myxozoa/crescimento & desenvolvimento Doenças Parasitárias em Animais/epidemiologia Rios/parasitologia Federação Russa/epidemiologia Esporos/crescimento & desenvolvimento Esporos/ultraestrutura [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180222 [Lr] Data última revisão: 180222 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180207 [St] Status: MEDLINE

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[PMID]: 29394019 [Au] Autor: Wei KS; Stella C; Wehmeyer KR; Christman J; Altemeier A; Spruell R; Wimalasena RL; Fadayel GM; Reilman RA; Motlagh S; Stoffolano PJ; Benzing K; Wickett RR [Ti] Título: Effects of season stratum corneum barrier function and skin biomarkers. [So] Source: J Cosmet Sci;67(3):185-203, 2016 May-Jun. [Is] ISSN: 1525-7886 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The skin on the lower legs of 25 female subjects was evaluated first in the winter, and then again in the summer of the same subjects. Barrier function was determined by measuring transepidermal water loss (TEWL), and skin hydration and dryness were evaluated by electrical measurements (Corneometer ® CM825) and visual grading. Stratum corneum (SC) was sampled using 10 sequential D-Squame sampling discs and analyzed for 2-pyrrolidone-5-carboxylic acid (PCA), keratin-1,10,11, interleukin 1α (IL-1α), interleukin 1 receptor antagonist (IL-1ra), selected ceramides, cholesterol, cholesterol sulfate, and selected free fatty acids. TEWL as well as the visual dryness grades were significantly lower in the summer while hydration was higher. PCA was significantly higher in the summer as were the keratins. The ratio IL-1ra:IL-1α, an indicator of skin inflammation, was significantly lower in the summer. The amount of protein removed by the tape strips was also significantly lower in summer indicating better SC cohesion. Among the SC lipids measured, total ceramides, individual ceramides, total fatty acids, and cholesterol were higher in summer compared to winter. Stearic acid and cholesterol sulfate were not significantly different between winter and summer. [Mh] Termos MeSH primário: Biomarcadores/metabolismo Epiderme/fisiologia Pele/metabolismo [Mh] Termos MeSH secundário: Adolescente Adulto Idoso Feminino Seres Humanos Meia-Idade Estações do Ano Fenômenos Fisiológicos da Pele Perda Insensível de Água Adulto Jovem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180215 [Lr] Data última revisão: 180215 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180203 [St] Status: MEDLINE

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[PMID]: 29288671 [Au] Autor: Zhang Y; Wang Y; Wu G; Zhang W; Wang X; Cai W; Zhang J; Han S; Li Y; Bai X; Shi J; Su L; Hu D [Ad] Endereço: Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, 710032, China. [Ti] Título: Prolonged skin grafts survival time by IFN-γ in allogeneic skin transplantation model during acute rejection through IFN-γ/STAT3/IDO pathway in epidermal layer. [So] Source: Biochem Biophys Res Commun;496(2):436-442, 2018 02 05. [Is] ISSN: 1090-2104 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Allogeneic skin transplantation is the life-saving therapy for multiple diseases, including extensive burn, large-scale trauma and certain post-surgical complications. However, acute rejection impedes clinical application of allogeneic skin transplantation. Although a lot of novel immunosuppressant drugs have been developed, there is still great need for ideal therapy with less complication and more therapeutic effects. Here, we found interferon gamma (IFN-γ) as an immunomodulatory cytokine prolonged the survival time of allografts from (8.50 ±â€¯1.517) days to (14.83 ±â€¯2.714) days at best. Indoleamine-2, 3-dioxygenase (IDO) has been proposed to play key roles in induction of immune tolerance. Using in vitro tissue culture and primary keratinocytes and fibroblasts, we investigated the regulatory effects of IFN-γ on the IDO expression. IFN-γ upregulated IDO expression through STAT3 phosphorylation and this upregulation was reduced by abolition of STAT3 phosphorylation through a STAT3 phosphorylation inhibitor. Interestingly, IFN-γ induced IDO expression predominately in epidermis rather than dermis. In consistent with these results, IFN-γ significantly triggered IDO expression in keratinocytes but not fibroblasts. Taken together, this suggests that IFN-γ might be a potential immunomodulatory drug in acute rejection and keratinocytes in epidermis may play a main role in immune tolerance after allogeneic skin transplantation. [Mh] Termos MeSH primário: Rejeição de Enxerto/prevenção & controle Sobrevivência de Enxerto Fatores Imunológicos/farmacologia Indolamina-Pirrol 2,3,-Dioxigenase/genética Interferon gama/farmacologia Fator de Transcrição STAT3/genética Transplante de Pele [Mh] Termos MeSH secundário: Doença Aguda Animais Derme/citologia Derme/efeitos dos fármacos Derme/imunologia Epiderme/citologia Epiderme/efeitos dos fármacos Epiderme/imunologia Fibroblastos/citologia Fibroblastos/efeitos dos fármacos Fibroblastos/imunologia Regulação da Expressão Gênica Rejeição de Enxerto/imunologia Rejeição de Enxerto/patologia Tolerância Imunológica/efeitos dos fármacos Indolamina-Pirrol 2,3,-Dioxigenase/imunologia Queratinócitos/citologia Queratinócitos/efeitos dos fármacos Queratinócitos/imunologia Masculino Camundongos Camundongos Endogâmicos BALB C Camundongos Endogâmicos C57BL Especificidade de Órgãos Fosforilação Fator de Transcrição STAT3/imunologia Transplante Homólogo [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (IDO1 protein, mouse); 0 (Immunologic Factors); 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase); 0 (STAT3 Transcription Factor); 0 (Stat3 protein, mouse); 82115-62-6 (Interferon-gamma) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180213 [Lr] Data última revisão: 180213 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171231 [St] Status: MEDLINE

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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