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Pesquisa : A10.336.482 [Categoria DeCS]
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  1 / 17724 MEDLINE  
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[PMID]:29325588
[Au] Autor:Nørstebø H; Rachah A; Dalen G; Rønningen O; Whist AC; Reksen O
[Ad] Endereço:Department of Production Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, PO Box 8146 Dep, 0033, Oslo, Norway. havard.norstebo@nmbu.no.
[Ti] Título:Milk-flow data collected routinely in an automatic milking system: an alternative to milking-time testing in the management of teat-end condition?
[So] Source:Acta Vet Scand;60(1):2, 2018 Jan 11.
[Is] ISSN:1751-0147
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Having a poor teat-end condition is associated with increased mastitis risk, hence avoiding milking machine settings that have a negative effect on teat-end condition is important for successful dairy production. Milking-time testing (MTT) can be used in the evaluation of vacuum conditions during milking, but the method is less suited for herds using automatic milking systems (AMS) and relationships with teat end condition is poorly described. This study aimed to increase knowledge on interpretation of MTT in AMS and to assess whether milk-flow data obtained routinely by an AMS can be useful for the management of teat-end health. A cross-sectional study, including 251 teats of 79 Norwegian Red cows milked by AMS was performed in the research herd of the Norwegian University of Life Sciences. The following MTT variables were obtained at teat level: Average vacuum level in the short milk tube during main milking (MTVAC), average vacuum in the mouthpiece chamber during main milking and overmilking, teat compression intensity (COMPR) and overmilking time. Average and peak milk flow rates were obtained at quarter level from the AMS software. Teat-end callosity thickness and roughness was registered, and teat dimensions; length, and width at apex and base, were measured. Interrelationships among variables obtained by MTT, quarter milk flow variables, and teat dimensions were described. Associations between these variables and teat-end callosity thickness and roughness, were investigated. RESULTS: Principal component analysis showed clusters of strongly related variables. There was a strong negative relationship between MTVAC and average milk flow rate. The variables MTVAC, COMPR and average and peak milk flow rate were associated with both thickness and roughness of the callosity ring. CONCLUSIONS: Quarter milk flow rate obtained directly from the AMS software was useful in assessing associations between milking machine function and teat-end condition; low average milk flow rates were associated with a higher likelihood of the teat having a thickened or roughened teat-end callosity ring. Since information on milk flow rate is readily available from the herd management system, this information might be used when evaluating causes for impaired teat-end condition in AMS.
[Mh] Termos MeSH primário: Calosidades/prevenção & controle
Indústria de Laticínios/métodos
Lactação/fisiologia
Glândulas Mamárias Animais/patologia
[Mh] Termos MeSH secundário: Animais
Calosidades/etiologia
Calosidades/patologia
Bovinos
Estudos Transversais
Indústria de Laticínios/instrumentação
Feminino
Análise de Componente Principal
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1186/s13028-018-0356-x


  2 / 17724 MEDLINE  
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[PMID]:29323924
[Au] Autor:Zhang T; Huang J; Yi Y; Zhang X; Loor JJ; Cao Y; Shi H; Luo J
[Ad] Endereço:Shanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi 712100, PR China.
[Ti] Título:Akt Serine/Threonine Kinase 1 Regulates de Novo Fatty Acid Synthesis through the Mammalian Target of Rapamycin/Sterol Regulatory Element Binding Protein 1 Axis in Dairy Goat Mammary Epithelial Cells.
[So] Source:J Agric Food Chem;66(5):1197-1205, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Akt serine/threonine kinase acts as a central mediator in the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway, regulating a series of biological processes. In lipid metabolism, Akt activation regulates a series of gene expressions, including genes related to intracellular fatty acid synthesis. However, the regulatory mechanisms of Akt in dairy goat mammary lipid metabolism have not been elaborated. In this study, the coding sequences of goat Akt1 gene were cloned and analyzed. Gene expression of Akt1 in different lactation stages was also investigated. For in vitro studies, a eukaryotic expression vector of Akt1 was constructed and transfected to goat mammary epithelial cells (GMECs), and specific inhibitors of Akt/mammalian target of rapamycin (mTOR) signaling were applied to GMECs. Results showed that Akt1 protein was highly conserved, and its mRNA was highly expressed in midlactation. In vitro studies indicated that Akt1 phosphorylation activated mTOR and subsequently enhanced sterol regulatory element binding protein 1 (SREBP1), thus increasing intracellular triacylglycerol content. Inhibition of Akt/mTOR signaling down-regulated the gene expression of lipogenic genes. Overall, Akt1 plays an important role in regulating de novo fatty acid synthesis in goat mammary epithelial cells, and this process probably is through the mTOR/SREBP1 axis.
[Mh] Termos MeSH primário: Ácidos Graxos/biossíntese
Cabras
Glândulas Mamárias Animais/metabolismo
Proteínas Proto-Oncogênicas c-akt/fisiologia
Proteína de Ligação a Elemento Regulador de Esterol 1/fisiologia
Serina-Treonina Quinases TOR/fisiologia
[Mh] Termos MeSH secundário: Animais
Epitélio/metabolismo
Regulação da Expressão Gênica/fisiologia
Lipogênese/genética
Proteínas Proto-Oncogênicas c-akt/genética
Transdução de Sinais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Sterol Regulatory Element Binding Protein 1); EC 2.7.1.1 (TOR Serine-Threonine Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05305


  3 / 17724 MEDLINE  
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[PMID]:29324859
[Au] Autor:He D; Mustafi D; Fan X; Fernandez S; Markiewicz E; Zamora M; Mueller J; Sachleben JR; Brady MJ; Conzen SD; Karczmar GS
[Ad] Endereço:Department of Radiology, The University of Chicago, Chicago, Illinois, United States of America.
[Ti] Título:Magnetic resonance spectroscopy detects differential lipid composition in mammary glands on low fat, high animal fat versus high fructose diets.
[So] Source:PLoS One;13(1):e0190929, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effects of consumption of different diets on the fatty acid composition in the mammary glands of SV40 T-antigen (Tag) transgenic mice, a well-established model of human triple-negative breast cancer, were investigated with magnetic resonance spectroscopy and spectroscopic imaging. Female C3(1) SV40 Tag transgenic mice (n = 12) were divided into three groups at 4 weeks of age: low fat diet (LFD), high animal fat diet (HAFD), and high fructose diet (HFruD). MRI scans of mammary glands were acquired with a 9.4 T scanner after 8 weeks on the diet. 1H spectra were acquired using point resolved spectroscopy (PRESS) from two 1 mm3 boxes on each side of inguinal mammary gland with no cancers, lymph nodes, or lymph ducts. High spectral and spatial resolution (HiSS) images were also acquired from nine 1-mm slices. A combination of Gaussian and Lorentzian functions was used to fit the spectra. The percentages of poly-unsaturated fatty acids (PUFA), mono-unsaturated fatty acids (MUFA), and saturated fatty acids (SFA) were calculated from each fitted spectrum. Water and fat peak height images (maps) were generated from HiSS data. The results showed that HAFD mice had significantly lower PUFA than both LFD (p < 0.001) and HFruD (p < 0.01) mice. The mammary lipid quantity calculated from 1H spectra was much larger in HAFD mice than in LFD (p = 0.03) but similar to HFruD mice (p = 0.10). The average fat signal intensity over the mammary glands calculated from HiSS fat maps was ~60% higher in HAFD mice than in LFD (p = 0.04) mice. The mean or median of calculated parameters for the HFruD mice were between those for LFD and HAFD mice. Therefore, PRESS spectroscopy and HiSS MRI demonstrated water and fat composition changes in mammary glands due to a Western diet, which was low in potassium, high in sodium, animal fat, and simple carbohydrates. Measurements of PUFA with MRI could be used to evaluate cancer risk, improve cancer detection and diagnosis, and guide preventative therapy.
[Mh] Termos MeSH primário: Dieta com Restrição de Gorduras
Dieta Hiperlipídica
Açúcares da Dieta
Ácidos Graxos/metabolismo
Glândulas Mamárias Animais/metabolismo
Espectroscopia de Prótons por Ressonância Magnética
[Mh] Termos MeSH secundário: Animais
Feminino
Frutose
Imagem por Ressonância Magnética
Glândulas Mamárias Animais/diagnóstico por imagem
Camundongos Transgênicos
Distribuição Aleatória
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Dietary Sugars); 0 (Fatty Acids); 30237-26-4 (Fructose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190929


  4 / 17724 MEDLINE  
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[PMID]:29278767
[Au] Autor:Chen Z; Shi H; Sun S; Luo J; Zhang W; Hou Y; Loor JJ
[Ad] Endereço:Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yang ling, Shaanxi 712100, PR China.
[Ti] Título:MiR-183 regulates milk fat metabolism via MST1 in goat mammary epithelial cells.
[So] Source:Gene;646:12-19, 2018 Mar 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The nutritional value of goat milk largely depends on its fatty acid content and composition. MicroRNAs (miRNAs) are a class of RNA molecules 18-25nt in length that regulate gene expression and play crucial roles in several biological processes, including fatty acid metabolism. In this study, we analyzed the correlation between differentially expressed miRNAs in goat mammary tissue and the fatty acid composition of goat milk by using Pearson correlations. Results revealed that levels of miR-183 were highly and positively correlated with the fatty acid content in the milk. In addition, we demonstrated that overexpression of miR-183 inhibits milk fat metabolism and inhibition of miR-183 promotes milk fat metabolism. Using Western blot, we demonstrate that MST1, one of the major elements of the Hippo signaling pathway, is a target of miR-183. Immunofluorescence assays revealed that miR-183 targets MST1 in the cytoplasm. In summary, data indicate that miR-183 inhibits the metabolism of milk fat by targeting the MST1 gene in the cytoplasm in goat mammary epithelial cells.
[Mh] Termos MeSH primário: Ácidos Graxos/metabolismo
Cabras/genética
Fator de Crescimento de Hepatócito/genética
Glândulas Mamárias Animais/citologia
MicroRNAs/genética
Leite/química
Proteínas Proto-Oncogênicas/genética
[Mh] Termos MeSH secundário: Animais
Contagem de Células
Linhagem Celular
Células Epiteliais/citologia
Células Epiteliais/metabolismo
Feminino
Perfilação da Expressão Gênica
Regulação da Expressão Gênica
Cabras/metabolismo
Metabolismo dos Lipídeos
Glândulas Mamárias Animais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (MicroRNAs); 0 (Proto-Oncogene Proteins); 0 (macrophage stimulating protein); 67256-21-7 (Hepatocyte Growth Factor)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171227
[St] Status:MEDLINE


  5 / 17724 MEDLINE  
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[PMID]:29281677
[Au] Autor:Mobuchon L; Le Guillou S; Marthey S; Laubier J; Laloë D; Bes S; Le Provost F; Leroux C
[Ad] Endereço:GABI, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
[Ti] Título:Sunflower oil supplementation affects the expression of miR-20a-5p and miR-142-5p in the lactating bovine mammary gland.
[So] Source:PLoS One;12(12):e0185511, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Oil supplementation in dairy cattle diets is used to modulate milk fat composition, as well as the expression of mammary lipogenic genes, whose regulation remains unclear. MiRNAs are small non-coding RNA considered as crucial regulators of gene expression, offering clues to explain the mechanism underlying gene nutriregulation. The present study was designed to identify miRNAs whose expression in the cow mammary gland is modulated by sunflower oil supplementation. MiRNomes were obtained using RNAseq technology from the mammary gland of lactating cows receiving a low forage diet, supplemented or not with 4% sunflower oil. Among the 272 miRNAs characterized, eight were selected for RT-qPCR validations, showing the significant down-regulation of miR-142-5p and miR-20a-5p by sunflower supplementation. These two miRNAs are predicted to target genes whose expression was reported as differentially expressed by sunflower supplementation. Among their putative targets, ELOVL6 gene involved in lipid metabolism has been studied. However, a first analysis did not show its significant down-regulation, in response to the over-expression of miR-142-5p, of miR-20a-5p, or both, in a bovine mammary epithelial cell line. However, a clearer understanding of the miRNA expression by lipid supplementation would help to decipher the regulation of lactating cow mammary gland in response to nutrition.
[Mh] Termos MeSH primário: Lactação
Glândulas Mamárias Animais/metabolismo
MicroRNAs/genética
Óleo de Girassol/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Bovinos
Feminino
Metabolismo dos Lipídeos/genética
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MicroRNAs); 0 (Sunflower Oil)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185511


  6 / 17724 MEDLINE  
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[PMID]:28745626
[Au] Autor:Stanko JP; Fenton SE
[Ad] Endereço:National Toxicology Program Laboratory, Division of the National Toxicology Program, National Institute of Environmental Health Sciences.
[Ti] Título:Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method.
[So] Source:J Vis Exp;(125), 2017 Jul 12.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An increasing number of studies are utilizing the rodent mammary gland as an endpoint for assessing the developmental toxicity of a chemical exposure. The effects these exposures have on mammary gland development are typically evaluated using either basic dimensional measurements or by scoring morphological characteristics. However, the broad range of methods for interpreting developmental changes could lead to inconsistent translations across laboratories. A common method of assessment is needed so that proper interpretations can be formed from data being compared across studies. The present study describes the application of the Sholl analysis method to quantify mammary gland branching characteristics. The Sholl method was originally developed for use in quantifying neuronal dendritic patterns. By using ImageJ, an open-source image analysis software package, and a plugin developed for this analysis, the mammary gland branching density and the complexity of a mammary gland from a peripubertal female rat were determined. The methods described here will enable the use of the Sholl analysis as an effective tool for quantifying an important characteristic of mammary gland development.
[Mh] Termos MeSH primário: Glândulas Mamárias Animais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Feminino
Glândulas Mamárias Animais/patologia
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180119
[Lr] Data última revisão:
180119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.3791/55789


  7 / 17724 MEDLINE  
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[PMID]:29189005
[Au] Autor:Tsugami Y; Matsunaga K; Suzuki T; Nishimura T; Kobayashi K
[Ad] Endereço:Laboratory of Cell and Tissue Biology, Research Faculty of Agriculture, Hokkaido University , North 9, West 9, 060-8589 Sapporo, Japan.
[Ti] Título:Phytoestrogens Weaken the Blood-Milk Barrier in Lactating Mammary Epithelial Cells by Affecting Tight Junctions and Cell Viability.
[So] Source:J Agric Food Chem;65(50):11118-11124, 2017 Dec 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During lactation, mammary epithelial cells (MECs) form the blood-milk barrier by less-permeable tight junctions (TJs) to prevent the leakage of milk components. Phytoestrogens affect the proliferation, differentiation, and apoptosis of MECs. However, it remains unclear whether phytoestrogens are involved in the blood-milk barrier. Therefore, we investigated the influence of phytoestrogens (coumestrol, genistein, and daidzein) by using an in vitro mouse-MEC-culture model. The results showed that coumestrol and genistein changed the expression of TJ proteins (claudins-3 and -4 and occludin), weakened barrier function, and reduced ß-casein production. Daidzein also weakened barrier function without inhibiting ß-casein production. Additionally, coumestrol and genistein induced apoptosis in MECs. These results indicate that phytoestrogens weaken the blood-milk barrier by directly affecting TJs and the cellular viability of lactating MECs in different ways.
[Mh] Termos MeSH primário: Cumestrol/farmacologia
Células Epiteliais/metabolismo
Genisteína/farmacologia
Isoflavonas/farmacologia
Glândulas Mamárias Animais/citologia
Leite/metabolismo
Fitoestrógenos/farmacologia
Junções Íntimas/metabolismo
[Mh] Termos MeSH secundário: Animais
Caseínas/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Células Epiteliais/citologia
Feminino
Seres Humanos
Lactação
Glândulas Mamárias Animais/irrigação sanguínea
Glândulas Mamárias Animais/efeitos dos fármacos
Glândulas Mamárias Animais/metabolismo
Camundongos
Camundongos Endogâmicos ICR
Junções Íntimas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caseins); 0 (Isoflavones); 0 (Phytoestrogens); 6287WC5J2L (daidzein); DH2M523P0H (Genistein); V7NW98OB34 (Coumestrol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04786


  8 / 17724 MEDLINE  
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[PMID]:28464792
[Au] Autor:Hou J; An X; Song Y; Cao B; Yang H; Zhang Z; Shen W; Li Y
[Ad] Endereço:Animal Engineering Branch, Yangling Vocational & Technical College, No. 10 Xinong Road, Yangling, Shaanxi, 712100, People's Republic of China.
[Ti] Título:Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages.
[So] Source:BMC Genet;18(1):38, 2017 05 02.
[Is] ISSN:1471-2156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: MicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods. RESULT: This study identified 131 differentially expressed miRNAs (P < 0.05 and log colostrum normalized expression (NE)/peak lactation NE > 1 or log colostrum NE/peak lactation NE < -1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P < 0.05 and log colostrum NE/peak lactation NE > 1 or log colostrum NE/peak lactation NE < -1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway. CONCLUSIONS: Our finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland.
[Mh] Termos MeSH primário: Colostro/química
Cabras/genética
Lactação/genética
Glândulas Mamárias Animais/crescimento & desenvolvimento
MicroRNAs/análise
Leite/química
[Mh] Termos MeSH secundário: Animais
Feminino
Perfilação da Expressão Gênica
Regulação da Expressão Gênica
Cabras/metabolismo
Sequenciamento de Nucleotídeos em Larga Escala
Glândulas Mamárias Animais/citologia
Glândulas Mamárias Animais/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171221
[Lr] Data última revisão:
171221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s12863-017-0498-2


  9 / 17724 MEDLINE  
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[PMID]:27771439
[Au] Autor:Goddard ET; Hill RC; Barrett A; Betts C; Guo Q; Maller O; Borges VF; Hansen KC; Schedin P
[Ad] Endereço:Department of Cell, Developmental and Cancer Biology, Oregon Health & Science University, Portland, OR, USA.
[Ti] Título:Quantitative extracellular matrix proteomics to study mammary and liver tissue microenvironments.
[So] Source:Int J Biochem Cell Biol;81(Pt A):223-232, 2016 12.
[Is] ISSN:1878-5875
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Normal epithelium exists within a dynamic extracellular matrix (ECM) that is tuned to regulate tissue specific epithelial cell function. As such, ECM contributes to tissue homeostasis, differentiation, and disease, including cancer. Though it is now recognized that the functional unit of normal and transformed epithelium is the epithelial cell and its adjacent ECM, we lack a basic understanding of tissue-specific ECM composition and abundance, as well as how physiologic changes in ECM impact cancer risk and outcomes. While traditional proteomic techniques have advanced to robustly identify ECM proteins within tissues, methods to determine absolute abundance have lagged. Here, with a focus on tissues relevant to breast cancer, we utilize mass spectrometry methods optimized for absolute quantitative ECM analysis. Employing an extensive protein extraction and digestion method, combined with stable isotope labeled Quantitative conCATamer (QconCAT) peptides that serve as internal standards for absolute quantification of protein, we quantify 98 ECM, ECM-associated, and cellular proteins in a single analytical run. In rodent models, we applied this approach to the primary site of breast cancer, the normal mammary gland, as well as a common and particularly deadly site of breast cancer metastasis, the liver. We find that mammary gland and liver have distinct ECM abundance and relative composition. Further, we show mammary gland ECM abundance and relative compositions differ across the reproductive cycle, with the most dramatic changes occurring during the pro-tumorigenic window of weaning-induced involution. Combined, this work suggests ECM candidates for investigation of breast cancer progression and metastasis, particularly in postpartum breast cancers that are characterized by high metastatic rates. Finally, we suggest that with use of absolute quantitative ECM proteomics to characterize tissues of interest, it will be possible to reconstruct more relevant in vitro models to investigate tumor-ECM dynamics at higher resolution.
[Mh] Termos MeSH primário: Microambiente Celular
Matriz Extracelular/metabolismo
Fígado/citologia
Glândulas Mamárias Animais/citologia
Proteômica
[Mh] Termos MeSH secundário: Animais
Feminino
Ratos
Ratos Sprague-Dawley
Reprodução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171217
[Lr] Data última revisão:
171217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161030
[St] Status:MEDLINE


  10 / 17724 MEDLINE  
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[PMID]:27771090
[Au] Autor:Weller MMDCA; Albino RL; Marcondes MI; Silva W; Daniels KM; Campos MM; Duarte MS; Mescouto ML; Silva FF; Guimarães SEF
[Ad] Endereço:Animal Science Department, Universidade Federal de Viçosa, Minas Gerais, Brazil 36570-000.
[Ti] Título:Effects of nutrient intake level on mammary parenchyma growth and gene expression in crossbred (Holstein × Gyr) prepubertal heifers.
[So] Source:J Dairy Sci;99(12):9962-9973, 2016 Dec.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers, such as CD36, ACCA, FASN, and ADIPOR1, was upregulated by nutrient intake level. Significant nutrient intake × time interactions for lipogenic genes during the experimental period indicated variable gene expression depending on the time point of prepubertal mammary gland development. Overall, our data suggest that enhancing nutrient intake increased body fat accumulation and lipogenesis in the mammary gland to the detriment of parenchyma growth. Moreover, increased lipogenesis in the parenchyma of HG heifers may indicate that fat accumulation occurred because of adipocyte hypertrophy and not differences in adipogenesis. The implications of these results for milk yield needs to be elucidated.
[Mh] Termos MeSH primário: Bovinos/fisiologia
Dieta/veterinária
Regulação da Expressão Gênica
Fígado/metabolismo
Glândulas Mamárias Animais/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Bovinos/genética
Bovinos/crescimento & desenvolvimento
Ingestão de Energia
Feminino
Glândulas Mamárias Animais/metabolismo
Tecido Parenquimatoso/crescimento & desenvolvimento
Tecido Parenquimatoso/metabolismo
Distribuição Aleatória
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE



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